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Abstract Present study analysed the therapeutic potential of traditionally acclaimed medicinal herb Nanorrhinum ramosissimum, using plant parts extracted with different solvents (10 mg/mL). Shoot extracts exhibited comparatively better antimicrobial properties, in comparison to root extracts. Total phenolic content was estimated, to ascertain its dependency on antioxidant properties of plant extracts. Antioxidant assay revealed promising results in comparison to IC50 value of standard ascorbic acid (52.2±0.07 µg/mL), for methanolic extracts of shoot (61.07±0.53 µg/mL and 64.33±0.33 µg/mL) and root (76.705±0.12 µg/mL and 89.73±0.28 µg/ mL) for in vivo and in vitro regenerants respectively. Correlation coefficient R2 values ranged between 0.90-0.95, indicating a positive correlation between phenolic contents and antioxidant activity. Plant extracts were also able to inhibit DNA oxidative damage again indicating their antioxidative potential. Antidiabetic potential was confirmed by alpha amylase inhibition assay where shoot methanolic extracts (invivo, in vitro) exhibited the best IC50 values (54.42±0.16 µg/mL, 66.09±0.12 µg/mL) in comparison to standard metformin (41.92±0.08 µg/mL). Ethanolic extracts of roots (in vitro, invivo) exhibited the relative IC50 values (88.97±0.32µg/mL,96.63±0.44 µg/mL) indicating that shoot parts had a better alpha amylase inhibition property; thus proving the herb’s bioactive potential and its prospective therapeutic source for curing various ailments. ramosissimum 10 (1 mg/mL. mgmL mg/mL . mg mL mg/mL) estimated IC IC5 52.2±0.07 522007 52 2 0 07 (52.2±0.0 µgmL , µg 61.07±0.53 6107053 61 53 (61.07±0.5 6433033 64 33 64.33±0.3 76.705±0.12 76705012 76 705 12 (76.705±0.1 8973028 89 73 28 89.73±0.2 respectively R 0.900.95, 090095 0.90 0.95, 90 95 0.90-0.95 activity invivo, invivo (invivo 54.42±0.16 5442016 54 42 16 (54.42±0.1 6609012 66 09 66.09±0.1 41.92±0.08 4192008 41 92 08 (41.92±0.0 µg/mL. 88.97±0.32µg/mL,96.63±0.44 8897032µgmL9663044 88 97 32µg 96 63 44 (88.97±0.32µg/mL,96.63±0.4 property herbs s ailments 1 ( 52.2±0.0 52200 5 (52.2±0. 61.07±0.5 610705 6 (61.07±0. 643303 3 64.33±0. 76.705±0.1 7670501 7 70 (76.705±0. 897302 8 89.73±0. 900 0.900.95 09009 090 0.9 095 0.95 9 0.90-0.9 54.42±0.1 544201 4 (54.42±0. 660901 66.09±0. 41.92±0.0 419200 (41.92±0. 88.97±0.32µg/mL,96.63±0.4 8897032µgmL966304 (88.97±0.32µg/mL,96.63±0. 52.2±0. 5220 (52.2±0 61.07±0. 61070 (61.07±0 64330 64.33±0 76.705±0. 767050 (76.705±0 89730 89.73±0 0.900.9 0900 0. 0.90-0. 54.42±0. 54420 (54.42±0 66090 66.09±0 41.92±0. 41920 (41.92±0 88.97±0.32µg/mL,96.63±0. 8897032µgmL96630 (88.97±0.32µg/mL,96.63±0 52.2±0 522 (52.2± 61.07±0 6107 (61.07± 6433 64.33± 76.705±0 76705 (76.705± 8973 89.73± 0.900. 0.90-0 54.42±0 5442 (54.42± 6609 66.09± 41.92±0 4192 (41.92± 88.97±0.32µg/mL,96.63±0 8897032µgmL9663 (88.97±0.32µg/mL,96.63± 52.2± (52.2 61.07± 610 (61.07 643 64.33 76.705± 7670 (76.705 897 89.73 0.900 0.90- 54.42± 544 (54.42 660 66.09 41.92± 419 (41.92 88.97±0.32µg/mL,96.63± 8897032µgmL966 (88.97±0.32µg/mL,96.63 52.2 (52. 61.07 (61.0 64.3 76.705 767 (76.70 89.7 54.42 (54.4 66.0 41.92 (41.9 88.97±0.32µg/mL,96.63 8897032µgmL96 (88.97±0.32µg/mL,96.6 52. (52 61.0 (61. 64. 76.70 (76.7 89. 54.4 (54. 66. 41.9 (41. 88.97±0.32µg/mL,96.6 8897032µgmL9 (88.97±0.32µg/mL,96. (5 61. (61 76.7 (76. 54. (54 41. (41 88.97±0.32µg/mL,96. 8897032µgmL (88.97±0.32µg/mL,96 (6 76. (76 (4 88.97±0.32µg/mL,96 (88.97±0.32µg/mL,9 (7 88.97±0.32µg/mL,9 (88.97±0.32µg/mL, 88.97±0.32µg/mL, (88.97±0.32µg/mL 88.97±0.32µg/mL