Abstract Objective To assess the efficacy of Phyllanthus emblica extract in alleviating halitosis and reducing the inflammatory response to halitosis-related bacteria. Methodology This investigation, using Phyllanthus emblica fruit extract (PE), involved four aspects. First, we evaluated the effect on growth and aggregation of halitosis-related bacteria, including Fusobacterium nucleatum, Porphyromonas gingivalis, and Solobacterium moorei, using a microdilution assay and scanning electron microscopy. Second, volatile sulfur compound (VSC) levels were measured on individuals with halitosis in randomized short-term (26 participants) and double-blind randomized long-term trials (18 participants in each group) after rinsing with PE for 3, 6, and 12 h, and 28 days. Third, we analyzed pro-inflammatory cytokine expression in TR146 cells using quantitative real-time PCR and enzyme-linked immunosorbent assays. Lastly, we assessed pro-inflammatory cytokine secretion and Toll-like receptor (TLR) 2 mRNA expression via the same experimental methods in a three-dimensional oral mucosal epithelial model (3D OMEM). Results PE extract dose-dependently inhibited the growth of F. nucleatum (50% inhibition concentration [IC50]=0.079%), P. gingivalis (IC50=0.65%), and S. moorei (IC50=0.07%) and effectively prevented bacterial aggregation. Furthermore, VSC contents decreased significantly at 3, 6, and 12 h after rinsing with 5% PE compared with those in the control. Long-term use of mouthwash containing 5% PE for 28 days led to a significant decrease in VSC contents. PE attenuated the F. nucleatum- or P. gingivalis-stimulated mRNA expression and protein release of interleukin (IL)-6 and IL-8 in TR146 cells. It also suppressed IL-8 and prostaglandin E2 secretion and TLR2 mRNA expression in F. nucleatum-induced OMEMs. Conclusion Our findings support the use of PE in oral care products to alleviate halitosis and it may reduce inflammation. halitosisrelated related bacteria investigation PE, , (PE) aspects First microscopy Second (VSC shortterm short term 26 (2 doubleblind double blind longterm long 18 (1 group 3 6 1 Third proinflammatory pro TR TR14 realtime real time enzymelinked enzyme linked assays Lastly Tolllike Toll like TLR (TLR threedimensional three dimensional 3D D OMEM. OMEM . OMEM) dosedependently dose dependently F 50% 50 (50 IC50=0.079%, IC500079 IC IC50 =0.079% 0 079 [IC50]=0.079%) P IC50=0.65%, IC50065 IC50=0.65% 65 (IC50=0.65%) S IC50=0.07% IC50007 07 (IC50=0.07% Furthermore 5 control Longterm Long gingivalisstimulated stimulated IL6 IL (IL)- IL8 8 IL- E nucleatuminduced induced OMEMs inflammation (PE ( TR1 (5 IC50=0.079% IC5 0079 =0.079 [IC50]=0.079% IC5006 IC50=0.65 (IC50=0.65% IC50=0.07 IC5000 (IC50=0.07 (IL) IC50=0.079 007 =0.07 [IC50]=0.079 IC500 IC50=0.6 (IC50=0.65 IC50=0.0 (IC50=0.0 (IL 00 =0.0 [IC50]=0.07 IC50=0. (IC50=0.6 (IC50=0. =0. [IC50]=0.0 IC50=0 (IC50=0 =0 [IC50]=0. IC50= (IC50= = [IC50]=0 (IC50 [IC50]= (IC5 [IC50] (IC [IC50 [IC5 [IC

Resumo: Este estudo emprega metodologia de teoria fundamentada, utilizando técnicas de codificação aberta, codificação axial e codificação seletiva, para conduzir uma análise sistemática da “Coleção de Padrões Decorativos Chineses”, de Pang Xunqin. Uma estrutura teórica é construída para explorar a fusão de padrões tradicionais chineses com o design de arte decorativa moderna. Como pioneira nesse domínio, a obra de Pang Xunqin não apenas preenche lacunas históricas nas artes decorativas chinesas, mas também oferece insights inovadores para a progressão da arte e do design chineses contemporâneos. A análise de codificação do estudo revela a integração hábil de padrões tradicionais em práticas de design modernas, por Pang Xunqin, aprimorando, assim, a identidade cultural e o vigor artístico. Além disso, um modelo dinâmico é introduzido, traçando caminhos inovadores para a evolução moderna de padrões tradicionais e promovendo sua disseminação cultural global e inovação. Esse modelo enriquece nossa compreensão da fusão de tradição e modernidade e apresenta uma nova perspectiva para a observação e a aplicação da cultura tradicional em cenários contemporâneos. Resumo fundamentada aberta seletiva Coleção Chineses, Chineses , Chineses” domínio chinesas contemporâneos modernas aprimorando assim artístico disso introduzido inovação

Abstract: This study employs grounded theory methodology, utilizing open coding, axial coding, and selective coding techniques, to conduct a systematic analysis of Pang Xunqin’s “Chinese Decorative Pattern Collection.” A theoretical framework is constructed to explore the fusion of traditional Chinese patterns with modern decorative art design. As a pioneer in this domain, Pang Xunqin’s oeuvre not only bridges historical gaps in Chinese decorative arts, but also offers innovative insights for the progression of contemporary Chinese art and design. The coding analysis of the study reveals the adept integration of traditional patterns into modern design practices by Pang Xunqin, thereby enhancing cultural identity and artistic vigor. Additionally, a dynamic model is introduced, charting innovative pathways for the modern evolution of traditional patterns and fostering their global cultural dissemination and innovation. This model enriches our comprehension of the amalgamation of tradition and modernity and presents a novel perspective for the observation and application of traditional culture in contemporary settings. Abstract methodology techniques Xunqins Xunqin s Collection. Collection domain arts vigor Additionally introduced innovation settings

The genetic diversity and genetic structure of three Chinese silkworm species Bombyx mori L., Antheraea pernyi Guérin-Meneville and Samia cynthia ricini Donovan were comparatively assessed based on RAPD markers. At the species level, A. pernyi and B. mori showed high levels of genetic diversity, whereas S. cynthia ricini showed low level of genetic diversity. However, at the strain level, A. pernyi had relatively highest genetic diversity and B. mori had lowest genetic diversity. Analysis of molecular variance (AMOVA) suggested that 60% and 72% of genetic variation resided within strains in A. pernyi and S. cynthia ricini, respectively, whereas only 16% of genetic variation occurred within strains in B. mori. In UPGMA dendrogram, individuals of A. pernyi and B. mori formed the strain-specific genetic clades, whereas those of S. cynthia ricini were distributed in a mixed way. The implications of these results for the conservation and utilization in breeding programs of three silkworm species are discussed.

Bombyx mori and Bombyx mandarina are morphologically and physiologically similar. In this study, we compared the nucleotide variations in the complete mitochondrial (mt) genomes between the domesticated silkmoth, B. mori, and its wild ancestors, Chinese B. mandarina (ChBm) and Japanese B. mandarina (JaBm). The sequence divergence and transition mutation ratio between B. mori and ChBm are significantly smaller than those observed between B. mori and JaBm. The preference of transition by DNA strands between B. mori and ChBm is consistent with that between B. mori and JaBm, however, the regional variation in nucleotide substitution rate shows a different feature. These results suggest that the ChBm mt genome is not undergoing the same evolutionary process as JaBm, providing evidence for selection on mtDNA. Moreover, investigation of the nucleotide sequence divergence in the A+T-rich region of Bombyx mt genomes also provides evidence for the assumption that the A+T-rich region might not be the fastest evolving region of the mtDNA of insects.