Abstract NCED1 (9-cis-epoxy carotenoid dioxygenase) plays important roles in controlling ABA levels and drought stress tolerance. The relationships between sequence polymorphisms of the TaNCED1 gene and drought resistance in wheat were analysed. Four allelic variations of the TaNCED1-5B generated to drought resistance were identified. Four groups of KASP (kompetitive allele-specific PCR) markers were developed based on the four alleles and were verified in a natural population of 311 wheat cultivars. The distribution of the four alleles of TaNCED1-5B in the natural population was clarified. Among the allelic variations, 84.62% of the wheat varieties with TT/-/C/-,TT/C/C/-,TT/-/C/G or TT/C/C/G haplotypes were dryland cultivars. The common feature of the above four haplotypes is that they all contain the TT (98) and C (343) alleles at the TaNCED1-5B-TT/CG (98) and TaNCED1-5B-C/T (343) loci, which indicated that these two locis had highly positive correlation with drought resistance in wheat. NCED 9cisepoxy cisepoxy 9 cis epoxy dioxygenase tolerance TaNCED analysed TaNCED15B TaNCEDB 5B B identified kompetitive allelespecific allele specific PCR 31 cultivars clarified 8462 84 62 84.62 TT//C/,TT/C/C/,TT//C/G TTCTTCCTTCG TT/ /C/ ,TT/C/C/ ,TT/ /C/G G TTCCG 98 (98 343 (343 TaNCED15BTT/CG TaNCED15BTTCG TaNCEDBTTCG TT/CG CG TaNCED15BC/T TaNCED15BCT TaNCEDBCT C/T T loci 3 846 8 6 84.6 /C TTCC ,TT/C/C ,TT (9 34 (34 TaNCED15BTT BTT BTTCG TTCG TaNCED15BC BC BCT CT 84. ( (3 TaNCEDBTT TaNCEDBC

RESUMEN Introducción: Uno de los focos de la investigación actual de los profesores de educación física de primera línea ha sido siempre la enseñanza optativa de los estudiantes universitarios que no practican deportes. La falta de tiempo de entrenamiento y la escasa formación de los alumnos hacen que muchos métodos profesionales de enseñanza del bádminton resulten imposibles para los cursos optativos. Objetivo: Estudiar el impacto del entrenamiento funcional en el rendimiento en bádminton de estudiantes universitarios. Métodos: Se seleccionaron aleatoriamente 44 estudiantes universitarios sedentarios y se dividieron equitativamente en grupo experimental y grupo de control. En el grupo experimental se utilizó la estrategia de entrenamiento funcional, mientras que el grupo de control utilizó el método tradicional de entrenamiento de bádminton. Tanto el grupo experimental como el de control entrenaron dos veces por semana, durante 60 minutos y durante 12 semanas. Los datos obtenidos se comparan, analizan y discuten estadísticamente. Resultados: En términos de la prueba FMS, la puntuación total del grupo experimental se optimizó en 5,61, y la puntuación total del grupo de control se optimizó en 3,20. En cuanto al rendimiento en bádminton, el grupo experimental mostró un aumento de 23,12 puntos, mientras que el grupo de control aumentó 16,75 puntos (P<0,01), lo que indica que hubo una diferencia muy significativa en los resultados. Conclusión: Este trabajo evidenció la importancia de promover el entrenamiento funcional, comparativamente superior a los métodos de enseñanza deportiva practicados actualmente. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción deportes optativos Objetivo Métodos 4 semana 6 1 semanas comparan estadísticamente Resultados FMS 561 5 61 5,61 320 3 20 3,20 2312 23 23,1 1675 16 75 16,7 P<0,01, P001 P P<0,01 , 0 01 (P<0,01) Conclusión actualmente II tratamiento 56 5,6 32 2 3,2 231 23, 167 7 16, P00 P<0,0 (P<0,01 5, 3, P0 P<0, (P<0,0 P<0 (P<0, P< (P<0 (P< (P

RESUMO Introdução: Um dos focos das pesquisas atuais dos professores de educação física na linha de frente tem sido sempre o ensino eletivo de estudantes universitários que não praticam esportes. A falta de tempo de treinamento e a deficiente formação dos estudantes tornam muitos métodos profissionais de ensino de badminton impossíveis de serem realizados em cursos optativos. Objetivo: Estudar o impacto do treinamento funcional sobre o desempenho de badminton dos estudantes universitários. Métodos: Foram selecionados aleatoriamente 44 universitários sedentários, divididos igualmente em grupo experimental e controle. No grupo experimental foi utilizada a estratégia de treinamento funcional, enquanto o grupo de controle usou o método tradicional de treinamento de badminton. Tanto o grupo experimental quanto o grupo controle treinaram duas vezes por semana, por 60 minutos e durante 12 semanas. Os dados obtidos comparados, analisados e discutidos estatisticamente. Resultados: Em termos de teste FMS, a pontuação total do grupo experimental foi otimizada em 5,61, e a pontuação total do grupo de controle foi otimizada em 3,20. Com relação ao desempenho do badminton, o grupo experimental apresentou um aumento de 23,12 pontos, enquanto que o grupo controle aumentou 16.75 pontos (P<0.01), indicando que houve uma diferença muito significativa dos resultados. Conclusão: Este trabalho evidenciou a importância da promoção do treinamento funcional, comparativamente superior aos métodos de ensino esportivo praticados atualmente. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução esportes optativos Objetivo Métodos 4 sedentários semana 6 1 semanas comparados estatisticamente Resultados FMS 561 5 61 5,61 320 3 20 3,20 2312 23 23,1 1675 16 75 16.7 P<0.01, P001 P P<0.01 , 0 01 (P<0.01) Conclusão atualmente II tratamento 56 5,6 32 2 3,2 231 23, 167 7 16. P00 P<0.0 (P<0.01 5, 3, P0 P<0. (P<0.0 P<0 (P<0. P< (P<0 (P< (P

ABSTRACT Introduction: One focus of current research by frontline physical education teachers has always been the elective teaching of college students who do not play sports. Lack of training time and poor student training make many professional badminton teaching methods impossible for elective courses. Objective: Study the impact of functional training on the badminton performance of college students. Methods: Forty-four sedentary college students were randomly selected and equally divided into the experimental and control group. The functional training strategy was used in the experimental group, while the control group used the traditional badminton training method. The experimental and control groups were trained twice weekly for 60 minutes and 12 weeks. The data obtained were compared, analyzed, and discussed statistically. Results: In terms of the FMS test, the total score of the experimental group was optimized by 5.61, and the total score of the control group was optimized by 3.20. Regarding badminton performance, the experimental group showed an increase of 23.12 points, while the control group increased 16.75 points (P<0.01), indicating a very significant difference in the results. Conclusion: This work evidenced the importance of promoting functional training, which is superior to the current sports teaching methods. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction courses Objective Methods Fortyfour Forty four method 6 1 weeks compared analyzed statistically Results test 561 5 61 5.61 320 3 20 3.20 2312 23 23.1 1675 16 75 16.7 P<0.01, P001 P P<0.01 , 0 01 (P<0.01) results Conclusion II outcomes 56 5.6 32 2 3.2 231 23. 167 7 16. P00 P<0.0 (P<0.01 5. 3. P0 P<0. (P<0.0 P<0 (P<0. P< (P<0 (P< (P

Pilot trials have suggested that repetitive transcranial magnetic stimulation (rTMS) may reduce limb spasticity in multiple sclerosis (MS). We carried out the current meta-analysis to synthesize currently available evidence regarding such correlation. Up to November 2022, five international electronic databases (Cochrane CENTRAL, PubMed, Embase, Web of Science, and CINAHL) and four Chinese electronic databases (CBM, CNKI, WanFang Data, and VIP) were systematically searched to identify randomized trials comparing active rTMS and sham stimulation in patients with MS-related spasticity. Two reviewers independently selected studies and extracted data on study design, quality, clinical outcomes, and time points measured. The primary outcome was clinical spasticity relief after intervention. Secondary outcomes included spasticity at the follow-up visit 2 weeks later and post-treatment fatigue. Of 831 titles found, we included 8 studies (181 participants) in the quantitative analysis. Pooled analyses showed that rTMS therapy was associated with significant spasticity relief in the early post-intervention period [standardized mean differences (SMD): -0.67; 95%CI: -1.12 to -0.21], but there was insufficient evidence for rTMS in reducing spasticity at the follow-up visit 2 weeks later (SMD: -0.17; 95%CI: -0.52 to 0.17) and fatigue (SMD: -0.26; 95%CI: -0.84 to 0.31). This evidence supports the recommendations to treat MS-related spasticity with rTMS, but underlines the need for further large randomized trials. (rTMS MS. MS . (MS) metaanalysis meta analysis correlation 2022 Cochrane CENTRAL PubMed Embase Science CINAHL CBM, CBM (CBM CNKI Data VIP MSrelated related design quality measured intervention followup follow up posttreatment post treatment 83 found 181 (18 participants postintervention standardized SMD (SMD) 0.67 067 0 67 -0.67 95%CI 95CI CI 95 1.12 112 1 12 -1.1 0.21, 021 0.21 , 21 -0.21] (SMD 0.17 017 17 -0.17 0.52 052 52 -0.5 0.26 026 26 -0.26 0.84 084 84 -0.8 0.31. 031 0.31 31 0.31) (MS 202 18 (1 0.6 06 6 -0.6 9 1.1 11 -1. 02 0.2 -0.21 0.1 01 -0.1 0.5 05 5 -0. -0.2 0.8 08 03 0.3 3 20 ( 0. 1. -1 -0 -

RESUMEN Introducción: El ejercicio físico de calidad de los jóvenes es un requisito básico fundamental para el desarrollo de la aptitud física en la enseñanza del baloncesto universitario. Objetivo: Reforzar el entrenamiento del ejercicio físico en jóvenes jugadores de baloncesto. Métodos: Se realizó una selección aleatoria de 50 estudiantes voluntarios para la investigación. Se analizó la fuerza y la calidad de los jugadores de baloncesto a diferentes edades. El grupo experimental fue sometido a un entrenamiento de fuerza específico, mientras que el grupo de control fue sometido a ejercicios físicos rutinarios. El periodo del experimento fue de 12 semanas y se recogieron los indicadores técnicos específicos de los atletas antes y después de finalizar el experimento. Los datos recogidos fueron tratados estadísticamente y discutidos a la luz de la literatura científica. Resultados: No se encontraron diferencias significativas entre los dos grupos en la puntuación de la prueba inicial (P>0,05). El rendimiento del grupo experimental en el ejercicio fue mejor que el del grupo de control después de 12 semanas (P<0,01). El grupo experimental mostró un mejor rendimiento que el grupo de control en el salto de pie, el reingreso de 15 metros y el salto frontal, corroborando los datos significativamente diferentes entre los dos grupos (P<0,01). Conclusión: Este estudio tenía como objetivo estudiar el papel único del entrenamiento de la fuerza en la preparación física de los jóvenes jugadores de baloncesto. El entrenamiento de fuerza especial afecta positivamente al entrenamiento muscular de las extremidades superiores en jóvenes jugadores de baloncesto. Las extremidades inferiores de los jugadores de baloncesto mostraron ganancias funcionales expresivas con el entrenamiento de fuerza especial. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento. Introducción universitario Objetivo Métodos 5 edades específico rutinarios 1 científica Resultados P>0,05. P005 P P>0,05 . 0 05 (P>0,05) P<0,01. P001 P<0,01 01 (P<0,01) pie frontal Conclusión II tratamiento P00 P>0,0 (P>0,05 P<0,0 (P<0,01 P0 P>0, (P>0,0 P<0, (P<0,0 P>0 (P>0, P<0 (P<0, P> (P>0 P< (P<0 (P> (P< (P

RESUMO Introdução: O exercício físico de qualidade dos jovens é requisito básico fundamental para o desenvolvimento da aptidão física no ensino do basquetebol universitário. Objetivo: Fortalecer o treinamento do exercício físico em jovens jogadores de basquetebol. Métodos: Efetuou-se a seleção aleatória de 50 estudantes voluntários para a pesquisa. Foi analisada a força e a qualidade dos jogadores de basquetebol em diferentes idades. O grupo experimental foi submetido a um treinamento de força específica, enquanto o grupo de controle foi submetido a exercícios físicos de rotina. O período do experimento foi de 12 semanas e os indicadores técnicos específicos dos atletas foram coletados antes e após a finalização do experimento. Os dados coletados foram tratados estatisticamente e discutidos à luz da literatura científica. Resultados: Não foi encontrada diferença significativa entre os dois grupos na pontuação inicial do teste (P>0,05). O desempenho do grupo experimental no exercício foi melhor que o do grupo de controle após as 12 semanas (P<0,01). O grupo experimental demonstrou um desempenho melhor que o do grupo controle no salto em pé, reentrada de 15 metros, e salto de frente, corroborando com os dados significativamente distintos entre os dois grupos (P<0,01). Conclusão: Este estudo teve como objetivo estudar o papel único do treinamento de força no treinamento físico dos jovens jogadores de basquetebol. O treinamento especial de força afeta positivamente o treinamento muscular dos membros superiores nos jovens jogadores de basquetebol. As extremidades inferiores dos jogadores de basquetebol evidenciaram ganhos funcionais expressivos com o treinamento especial de força. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento. Introdução universitário Objetivo Métodos Efetuouse Efetuou se 5 pesquisa idades específica rotina 1 científica Resultados P>0,05. P005 P P>0,05 . 0 05 (P>0,05) P<0,01. P001 P<0,01 01 (P<0,01) pé metros frente Conclusão II tratamento P00 P>0,0 (P>0,05 P<0,0 (P<0,01 P0 P>0, (P>0,0 P<0, (P<0,0 P>0 (P>0, P<0 (P<0, P> (P>0 P< (P<0 (P> (P< (P

ABSTRACT Introduction: Quality exercise training of young people is a fundamental basic requirement for developing physical fitness in college basketball teaching. Objective: Enhance exercise training in young basketball players. Methods: A random selection of 50 student volunteers was made for this research. The strength and quality of basketball players of different ages were analyzed. The experimental group was subjected to specific strength training, while the control group was subjected to routine physical exercises. The experiment period was 12 weeks, and the specific technical indicators of the athletes were collected before and after the end of the experiment. The collected data were statistically treated and discussed in light of the scientific literature. Results: No significant difference was found between the two groups in the initial test score (P>0.05). The experimental group’s performance in exercise was better than the control group after 12 weeks (P<0.01). The experimental group demonstrated better performance than the control group in the standing jump, 15-meter reentry, and forward jump, corroborating the significantly different data between the two groups (P<0.01). Conclusion: This study aimed to study the unique role of strength training in the physical training of young basketball players. Special strength training positively affects upper limb muscle training in young basketball players. The lower extremities of basketball players showed expressive functional gains with special strength training. Level of evidence II; Therapeutic studies - investigation of treatment outcomes. Introduction teaching Objective Methods 5 research analyzed exercises 1 literature Results P>0.05. P005 P P>0.05 . 0 05 (P>0.05) s P<0.01. P001 P<0.01 01 (P<0.01) jump 15meter meter 15 reentry Conclusion II outcomes P00 P>0.0 (P>0.05 P<0.0 (P<0.01 P0 P>0. (P>0.0 P<0. (P<0.0 P>0 (P>0. P<0 (P<0. P> (P>0 P< (P<0 (P> (P< (P

Abstract Purpose: Myocardial ischemia/reperfusion (Ml/R) injury is a leading cause of damage in cardiac tissues, with high rates of mortality and disability. Biochanin A (BCA) is a main constituent of Trifolium pratense L. This study was intended to explore the effect of BCA on Ml/R injury and explore the potential mechanism. Methods: In vivo MI/R injury was established by transient coronary ligation in Sprague-Dawley rats. Triphenyltetrazolium chloride staining (TTC) was used to measure myocardial infarct size. ELISA assay was employed to evaluate the levels of myocardial enzyme and inflammatory cytokines. Western blot assay was conducted to detect related protein levels in myocardial tissues. Results: BCA significantly ameliorated myocardial infarction area, reduced the release of myocardial enzyme levels including aspartate transaminase (AST), creatine kinase (CK-MB) and lactic dehydrogenase (LDH). It also decreased the production of inflammatory cytokines (IL-1β, IL-18, IL-6 and TNF-α) in serum of Ml/R rats. Further mechanism studies demonstrated that BCA inhibited inflammatory reaction through blocking TLR4/NF-kB/NLRP3 signaling pathway. Conclusion: The present study is the first evidence demonstrating that BCA attenuated Ml/R injury through suppressing TLR4/NF-kB/NLRP3 signaling pathway-mediated anti-inflammation pathway.

Abstract Ultrasonic-assisted extraction technique was used to extract phenolic compounds from Phyllanthus urinaria and response surface methodology was used to optimize the extraction parameters including ultrasonic time (X1), ethanol concentration (X2) and solid-liquid ratio (X3). A mathematical model of the tree parameters for the high extraction yield (Y) was established. Antioxidant activity of the phenolic compounds was investigated by DPPH free radicals, ABTS free radicals, hydroxyl free radicals and reducing power assay with vitamin C as a control. The results showed that the independent variables (X1, X2 and X3) and the interaction terms (X1X3 and X2X3) had a significant effect on the extraction yield. The effect of the variables on the extraction yield was in the order of X3, X1 and X2. The optimal extraction process was X1 of 34 min, X2 of 46.5% and X3 of 1:63 g/mL, and the extraction yield was 9.59%, which was well matched with the predicted value. The antioxidant activity assay indicated that the phenolic compounds from Phyllanthus urinaria have a good antioxidant activity.

Abstract Recently, the rs1042713 G > A and rs1042714 C > G polymorphisms in the beta-2 adrenergic receptor (ADRB2) gene were shown to be related to atherosclerosis diseases. Therefore, we performed a systemic meta-analysis to determine whether the two functional polymorphisms are related to the risk of myocardial infarction (MI) and coronary artery disease (CAD). We identified published studies that are relevant to our topic of interest. Seven case-control studies, with a total of 6,843 subjects, were incorporated into the current meta-analysis. Our analysis showed a higher frequency of rs1042713 G > A variant in patients with MI or CAD compared to healthy controls. A similar result was also obtained with the rs1042714 C > G variant under both the allele and dominant models. Ethnicity-stratified subgroup analysis suggested that the rs1042714 C > G variant correlated with an increased risk of the two diseases in both Asians and Caucasians, while rs1042713 G > A only contributes to the risk of two diseases in Asians. In the disease type-stratified subgroups, the frequencies of both the rs1042713 G > A and rs1042714 C > G variants were higher in the cases than in the controls in both the MI and CAD subgroups. Collectively, our data contribute towards understanding the correlation between the rs1042713 G > A and rs1042714 C > G polymorphisms in ADRB2 and the susceptibility to MI and CAD.

Investigating the endophytic bacterial community in special moss species is fundamental to understanding the microbial-plant interactions and discovering the bacteria with stresses tolerance. Thus, the community structure of endophytic bacteria in the xerophilous moss Grimmia montana were estimated using a 16S rDNA library and traditional cultivation methods. In total, 212 sequences derived from the 16S rDNA library were used to assess the bacterial diversity. Sequence alignment showed that the endophytes were assigned to 54 genera in 4 phyla (Proteobacteria, Firmicutes, Actinobacteria and Cytophaga/Flexibacter/Bacteroids). Of them, the dominant phyla were Proteobacteria (45.9%) and Firmicutes (27.6%), the most abundant genera included Acinetobacter, Aeromonas, Enterobacter, Leclercia, Microvirga, Pseudomonas, Rhizobium, Planococcus, Paenisporosarcina and Planomicrobium. In addition, a total of 14 species belonging to 8 genera in 3 phyla (Proteobacteria, Firmicutes, Actinobacteria) were isolated, Curtobacterium, Massilia, Pseudomonas and Sphingomonas were the dominant genera. Although some of the genera isolated were inconsistent with those detected by molecular method, both of two methods proved that many different endophytic bacteria coexist in G. montana. According to the potential functional analyses of these bacteria, some species are known to have possible beneficial effects on hosts, but whether this is the case in G. montana needs to be confirmed.

Cigarette smoke is known to be a serious health risk factor and considered reproductively toxic. In the current study, we investigated whether constituents of cigarette smoke, pyrazine, 2-ethylpyridine, and 3-ethylpyridine, adversely affect reproductive functioning such as oocyte maturation and sperm capacitation. Our findings indicated that three smoke components were involved in retardation of oocyte maturation in a dose-dependent manner and the lowest-observed-adverse-effect level (LOAEL) was determined to be 10-10M. However, individual smoke components administrated at the LOAEL did not attenuate oocyte maturation, demonstrating that all three toxicants were equally required for the observed growth impairment. When exposed to all three components at 10-10M during in vitro capacitation, murine sperm lost forward progression and were unable to show adequate hyperactivation, which is indicative of the incompletion of the capacitation process. Only sperm administrated with 3-ethylpyridine alone showed significant reduction in capacitation status, suggesting the chemical is the one responsible for disrupting sperm capacitation. Taken together, this is the first report that documents the effect of cigarette smoke components on oocyte maturation and sperm capacitation. The present findings demonstrate the adverse effects of smoke constituents of mammalian reproduction and the differences in sensitivity to smoke components between male and female gametes. Since both processes take place in the female reproductive system, our data provide new insights into deleterious consequences of maternal exposure to cigarette smoke.

The MYF5 gene is first inducibly expressed in muscle cell during embryonic muscle development and plays an important role in regulating the differentiation of skeletal muscle precursors. In this study we used PCR-RFLP to investigate two pig (Sus scrofa) populations (n = 302) for two MYF5 gene polymorphisms, a previously unreported novel Met-Leu shift single nucleotide polymorphism (SNP) MYF5/Hsp92II located on exon 1 and the previously identified intron 1 MYF5/HinfI SNP. Haplotype and association analysis showed that haplotypes of the two SNPs were significantly associated with drip loss rate (DLR, p < 0.05), water holding capacity (WHC, p < 0.05), biceps femoris meat color value (MCV2, p < 0.05), biceps femoris marbling score (MM2, p < 0.01), longissimus dorsi intramuscular fat percentage (IMF, p < 0.01) and longissimus dorsi Water moisture content (WM, p < 0.01) in the population 2. However, further studies are needed to confirm these preliminary results.