Abstract Objectives: Lung cancer was one of the most common malignancies around the world. It has great significance in to search for the mechanism of occurrence and development of lung cancer. LIM Domain Binding protein 2 (LDB2) belongs to the LIM-domain binding family, it can be used as a binding protein that combined with other transcription factors to form the transcription complex for regulating the expression of target genes. The expression of microRNA-96-5p (miR-96-5p) has been investigated in various tumors. The aim of this study is to investigate the potential role of LDB2 and miR-96-5p in lung cancer. Methods: Real-time quantitative PCR was applied to detect the expression of LDB2 and miR-96-5p. The proliferation, invasion, and metastasis of H1299 cells were analyzed by CCK8, transwell, and wound healing assay after LDB2 or miR-96-5p transfection. Luciferase activities assay and western blot were used to reveal the targeted regulation between LDB2 and miR-96-5p. Results: Here the authors found LDB2 was down-regulated in lung cancer tissues and negatively correlated with miR-96-5p expression, it could promote or inhibit the proliferation, invasion and metastasis of H1299 cells after LDB2 knockdown or overexpression and regulate the expression of cyclinD1, MMP9, Bcl-2, and Bax via ERK1/2 signaling pathway. Furthermore, miR-96-5p exerted its function by directly binding to 3′-UTR of LDB2 and regulating expression of LDB2. miR-96-5p could promote the proliferation, invasion, and metastasis of H1299 cells. Conclusion: These findings demonstrate that LDB2 can act as a new regulator to inhibit cell proliferation, invasion, and metastasis via the ERK1/2 signaling pathway, and miR-96-5p may be a potential promising molecular by targeting LDB2 in lung cancer. Objectives world LDB (LDB2 LIMdomain domain family genes microRNA965p microRNAp microRNA 96 5p p miR965p miRp miR (miR-96-5p tumors Methods Realtime Real time miR965p. 5p. proliferation H H129 CCK8 CCK transwell transfection Results downregulated down regulated cyclinD1 cyclinD MMP9 MMP Bcl2, Bcl2 Bcl 2, Bcl-2 ERK12 ERK ERK1 ERK1/ pathway Furthermore 3′UTR 3UTR UTR 3′ 3 Conclusion (LDB 9 H12 Bcl- H1

RESUMEN Objetivo: Investigar la eficacia de la medicina tradicional china (MTC) en el tratamiento de la incontinencia urinaria de esfuerzo (IUE) femenina. Método: Se llevaron a cabo búsquedas de artículos publicados hasta septiembre de 2022 en las bases de datos PubMed, Cochrane, Web of Science, Embase, CNKI, Wanfang y VIP. Las variables se analizaron mediante la diferencia de medias ponderada (DMP), la diferencia de medias estandarizada (DME), la razón de momios (RM) y el intervalo de confianza (IC) del 95%. Resultados: Este estudio incluyó ocho investigaciones con la participación de 744 pacientes. Los resultados indican que la terapia de medicina tradicional china (MTC) presentó mejoras significativas en los resultados clínicos de pacientes con incontinencia urinaria de esfuerzo (IUE) (RM = 2.90, IC del 95%: 1.92–4.37, P = 0.000), al reducir la puntuación del Cuestionario Internacional sobre la Incontinencia y su impacto en la calidad de vida (ICIQ-SF) (DMP = –2.41, IC del 95%: –2.83– –1.98, P = 0.000), así como la disminución del volumen de orina a la 1 hora de fuga de la almohadilla urinaria (DMP = –1.86, IC del 95%: –2.23– –1.49, P = 0.000) y el aumento de la presión de cierre uretral máxima (PCUM) (DME = 0.86, IC del 95%: 0.61–1.11, P = 0.000). Conclusión: La terapia de medicina tradicional china (MTC) resulta efectiva en la mejora de los síntomas de la incontinencia urinaria, la urodinámica y la calidad de vida en pacientes con incontinencia urinaria de esfuerzo (IUE). Este artículo ofrece una referencia para la aplicación de la terapia de MTC en mujeres con incontinencia urinaria. Objetivo (MTC IUE (IUE femenina Método 202 PubMed Cochrane Science Embase CNKI VIP DMP, DMP , (DMP) DME, DME (DME) RM (IC 95 95% Resultados 74 290 2 90 2.90 192437 92 4 37 1.92–4.37 0.000, 0000 0.000 0 000 ICIQSF ICIQ SF (ICIQ-SF 241 41 –2.41 283 83 –2.83 198 98 –1.98 186 86 –1.86 223 23 –2.23 149 49 –1.49 PCUM (PCUM 086 0.86 061111 61 11 0.61–1.11 0.000. . Conclusión IUE. 20 9 7 29 2.9 19243 3 1.92–4.3 0.00 00 24 –2.4 28 8 –2.8 19 –1.9 18 –1.8 22 –2.2 14 –1.4 08 0.8 06111 6 0.61–1.1 2. 1924 1.92–4. 0.0 –2. –1. 0. 0611 0.61–1. 192 1.92–4 –2 –1 061 0.61–1 1.92– – 06 0.61– 1.92 0.61 1.9 0.6 1.

RESUMO Objetivo: Investigar a eficácia da medicina tradicional chinesa (MTC) no tratamento da incontinência urinária de esforço (IUE) feminina. Método: Foram realizadas pesquisas de artigos publicados até setembro de 2022 nas bases de dados PubMed, Cochrane, Web of Science, Embase, CNKI, Wanfang e VIP. As variáveis foram analisadas por meio da diferença de média ponderada (DMP), da diferença média padronizada (DMP), da razão de chances (RC) e do intervalo de confiança (IC) de 95%. Resultados: Esta pesquisa envolveu oito estudos, contando com a participação de 744 pacientes. Os resultados indicam que a terapia da medicina tradicional chinesa (MTC) apresentou melhorias significativas nos resultados clínicos de pacientes com incontinência urinária de esforço (IUE) (RM = 2.90, IC de 95%: 1.92–4.37, P = 0.000), ao reduzir a pontuação do Questionário Internacional sobre a Incontinência Urinária – Versão Breve (ICIQ-SF) (Diferença de Média Ponderada = –2.41, IC de 95%: –2.83– –1.98, P = 0.000), assim como na diminuição do volume de urina na 1 hora de vazamento do absorvente urinário (Diferença de Média Ponderada = –1.86, IC de 95%: –2.23– –1.49, P = 0.000) e no aumento da pressão máxima de encerramento uretral (PMCU) (Diferença Média Padronizada = 0.86, IC de 95%: 0.61–1.11, P = 0.000). Conclusão: A terapia da medicina tradicional chinesa (MTC) é eficaz na melhoria dos sintomas da incontinência urinária, na urodinâmica e na qualidade de vida em pacientes com incontinência urinária de esforço (IUE). Este artigo fornece uma referência para a aplicação da terapia da MTC em mulheres com incontinência urinária. Objetivo (MTC IUE (IUE feminina Método 202 PubMed Cochrane Science Embase CNKI VIP DMP, DMP , (DMP) RC (RC (IC 95 95% Resultados estudos 74 RM 290 2 90 2.90 192437 92 4 37 1.92–4.37 0.000, 0000 0.000 0 000 ICIQSF ICIQ SF (ICIQ-SF Diferença 241 41 –2.41 283 83 –2.83 198 98 –1.98 186 86 –1.86 223 23 –2.23 149 49 –1.49 PMCU (PMCU 086 0.86 061111 61 11 0.61–1.11 0.000. . Conclusão IUE. 20 (DMP 9 7 29 2.9 19243 3 1.92–4.3 0.00 00 24 –2.4 28 8 –2.8 19 –1.9 18 –1.8 22 –2.2 14 –1.4 08 0.8 06111 6 0.61–1.1 2. 1924 1.92–4. 0.0 –2. –1. 0. 0611 0.61–1. 192 1.92–4 –2 –1 061 0.61–1 1.92– 06 0.61– 1.92 0.61 1.9 0.6 1.

ABSTRACT Objectives: To investigate the efficacy of traditional Chinese medicine (TCM) in the treatment of female stress urinary incontinence (SUI). Method: PubMed, Cochrane, Web of Science, Embase, CNKI, Wanfang, and VIP databases were searched for articles published up to September 2022. Variables were analyzed using weighted mean difference (WMD), standardized mean difference (SMD), odds ratios (OR), and 95% confidence interval (CI). Results: Eight studies containing 744 patients were included in this study. The results demonstrate that TCM therapy had more advantages in improving the clinical outcome of SUI patients (OR = 2.90, 95%CI:1.92–4.37, P = 0.000), reducing the International Consultation on Incontinence Questionnaire Short-Form (ICIQ-SF) score (WMD = –2.41, 95%CI:–2.83– –1.98, P = 0.000), reducing 1-h urinary pad leakage urine volume (WMD = –1.86, 95%CI:–2.23– –1.49, P = 0.000) and increasing Maximum urethral closure pressure (MUCP) (SMD = 0.86, 95%CI: 0.61–1.11, P = 0.000). Conclusion: TCM therapy is effective in improving urinary incontinence symptoms, urodynamics, and quality of life in patients with SUI. This article provides a reference for the application of TCM therapy in women with urinary incontinence. Objectives (TCM . (SUI) Method PubMed Cochrane Science Embase CNKI Wanfang 2022 WMD, WMD , (WMD) SMD, SMD (SMD) OR, OR (OR) 95 CI. CI (CI) Results 74 study 290 2 90 2.90 95%CI1.92–4.37, 95CI192437 95%CI 1.92–4.37, 1 92 4 37 95%CI:1.92–4.37 0.000, 0000 0.000 0 000 ShortForm Short Form ICIQSF ICIQ SF (ICIQ-SF 241 41 –2.41 95%CI–2.83– 95CI283 –2.83– 83 95%CI:–2.83 198 98 –1.98 1h h 186 86 –1.86 95%CI–2.23– 95CI223 –2.23– 23 95%CI:–2.23 149 49 –1.49 MUCP (MUCP 086 0.86 95CI 061111 61 11 0.61–1.11 0.000. Conclusion symptoms urodynamics (SUI 202 9 (CI 7 29 2.9 CI1 95%CI1.92–4.37 95CI19243 192437 1.92–4.37 3 95%CI:1.92–4.3 0.00 00 24 –2.4 95%CI–2.83 95CI28 283 –2.83 8 95%CI:–2.8 19 –1.9 18 –1.8 95%CI–2.23 95CI22 223 –2.23 95%CI:–2.2 14 –1.4 08 0.8 06111 6 0.61–1.1 20 2. 95%CI1.92–4.3 95CI1924 19243 1.92–4.3 95%CI:1.92–4. 0.0 –2. 95%CI–2.8 95CI2 28 –2.8 95%CI:–2. –1. 95%CI–2.2 22 –2.2 0. 0611 0.61–1. 95%CI1.92–4. 95CI192 1924 1.92–4. 95%CI:1.92–4 –2 95%CI–2. 95%CI:–2 –1 061 0.61–1 95%CI1.92–4 95CI19 192 1.92–4 95%CI:1.92– – 95%CI–2 95%CI:– 06 0.61– 95%CI1.92– 95CI1 1.92– 95%CI:1.92 95%CI– 0.61 95%CI1.92 1.92 95%CI:1.9 0.6 95%CI1.9 1.9 95%CI:1. 95%CI1. 1. 95%CI:1 95%CI1

Resumen El macizo de Chirripó en la Cordillera de Talamanca, Costa Rica, es uno de los pocos sitios en América Central que conserva evidencia de glaciación en el pasado. Mapeamos formas de relieve glaciales en esta área usando imágenes de alta resolución de Google Earth y un modelo de elevación digital (DEM) de la Misión Topográfica de Radar Shuttle de 30 m. Observaciones previamentes de campo, notas de campo, fotografías y mapas de formas de relieve se usaron para verificar y refinar los límites del relieve delineados. El mapa compuesto incluye morrenas laterales y terminales, laderas, valles glaciares, lagos glaciales, nunataks, arêtes, y cuernos. Las extensiones glaciales durante tres etapas propuestas se mapearon en el Valle de las Morrenas, un importante valle orientado hacia el norte en el macizo, basado en reconstrucciones previas. Las características glaciales mapeadas cubren aproximadamente 22,1 km2, que representan la extensión mínima de la glaciación máxima durante el período del último glacial máximo (LGM). Este mapa refinado proporciona un importante conjunto de datos para ayudar a reconstruir el cambio climático del Cuaternario en este altiplano tropical.

Abstract The Chirripó massif in the Cordillera de Talamanca, Costa Rica, is one of a few sites in Central America that preserve evidence of past glaciation. We mapped glacial landforms in this area using Google Earth high-resolution imagery and a 30 m Shuttle Radar Topography Mission (SRTM) digital elevation model (DEM). Prior field observations, field notes, photographs, and landform maps were used to check and refine the delineated landform boundaries. The composited map includes lateral and terminal moraines, tills, glacial valleys, glacial lakes, nunataks, arêtes, and horns. Glacial extents during three proposed stages were mapped in the Valle de las Morrenas, a major north-facing valley on the massif, based on previous reconstructions. The mapped glacial features cover roughly 22.1 km2, representing the minimum extent of the rnaximum glaciation during the Last Glacial Maximum (LGM). This refined map provides an important dataset to help reconstruct Quaternary climate change in this tropical highland.