Com o objetivo de aprimorar as propriedades eletroquímicas da liga do tipo AB3 La0,78Mg0,22Ni2,67Mn0,11Al0,11Co0,52 como eletrodo negativo de bateria Ni-MH, seus compósitos relacionados La0,78Mg0,22Ni2,67Mn0,11Al0,11Co0,52-x wt.% M1Ni3,5Co0,6Mn0,4Al0,5 (x = 0, 10, 20, 30) foram preparados. A análise por difratometria de raios X (XRD) revelou que os compósitos consistem principalmente das fases LaNi5 e La2Ni7. Apesar da pequena diminuição na capacidade de descarga máxima, o rendimento do ciclo foi significativamente melhorado. Experimentos de polarização linear (LP), polarização anódica (AP) e descarga de potencial degrau revelaram um aumento inicial da cinética eletroquímica que na sequência diminui com o aumento de x.

For improving the electrochemical properties of nonstoichiometric AB3-type La0.78Mg0.22Ni2.67Mn0.11Al0.11Co0.52 alloy as negative electrode of Ni-MH battery, its related composites La0.78Mg0.22Ni2.67Mn0.11Al0.11Co0.52-x wt.% M1Ni3.5Co0.6Mn0.4Al0.5 (x = 0, 10, 20, 30) were prepared. Analysis by X-ray diffractometry (XRD) revealed that the composites consist mainly of LaNi5 and La2Ni7 phases. Despite the small decrease in the maximum discharge capacity, the cycle performance was significantly enhanced. Linear polarization (LP), anodic polarization (AP) and potential step discharge experiments revealed that the electrochemical kinetics increases first and then decreases with increasing x.

BmPLV-Z is the abbreviation for Bombyx mori parvo-like virus (China isolate). This is a novel virus with two single-stranded linear DNA molecules, viz., VD1 (6543 bp) and VD2 (6022 bp), which are encapsidated respectively into separate virions. Analysis of the deduced amino acid sequence of VD1-ORF4 indicated the existence of a putative DNA-polymerase with exonuclease activity, possibly involved in the replication of BmPLV-Z. In the present study, a recombinant baculovirus was constructed to express the full length of the protein encoded by the VD1-ORF4 gene (3318 bp). In addition, a 2163-bp fragment amplified from the very same gene was cloned into prokaryotic expression vector pET-30a and expressed in E.coli Rosetta 2 (DE3) pLysS. The expressed fusion protein was employed to immunize New Zealand white rabbits for the production of an antiserum, afterwards used for examining the expression of the protein encoded by VD1-ORF4 gene in Sf-9 cells infected with recombinant baculovirus. Western blot analysis of extracts from thus cells infected revealed a specific band of about 120 kDa, thereby indicating that the full length protein encoded by the VD1-ORF4 gene had been successfully and stably expressed in Sf-9 cells.