Abstract Objectives The pathological mechanisms of patients with Renal Cell Carcinoma (RCC) remain defined. This study aimed to evaluate relationships between the landscape of gene mutations and their clinical significance in RCC patients. Methods Tissue and peripheral blood samples of 42 patients with RCC were collected and performed for the Next Generation Sequencing (NGS) with Geneseeq PrimeTM 425-gene panel probes. Their landscapes of gene mutation were analyzed. We also carried out an evaluation of Tumor-Node-Metastasis (TNM) staging, RENAL nephelometry score, surgery, and targeted drug treatment of patients. Then we compared the correlations of landscape in gene mutations and the prognosis. Results The most common gene alternations, including BAP1, PBRM1, SETD2, CSF1R, NPM1, EGFR, POLE, RB1, and VHL genes, were identified in tissue and blood samples of 75% of patients. EGFR, POLE, and RB1 gene mutations frequently occurred in relapsed and metastatic patients. BAP1, CCND2, KRAS, PTPN11, ERBB2/3, JAK2, and POLE were presented in the patients with > 9 RENAL nephelometry score. Univariable analysis indicated that SETD2, BAP1, and PBRM1 genes were key factors for Disease-Free Survival (DFS). Multivariable analysis confirmed that mutated SETD1, NPM1, and CSF1R were critical factors for the Progression Free Survival (PFS) of RCC patients with target therapy. Conclusions Wild-type PBRM1 and mutated BAP1 in patients with RCC were strongly associated with the outcomes of the patient. The PFS of the patients with SETD2, NPM1, and CSF1R mutations were significantly shorter than those patients without variants. (RCC defined 4 NGS (NGS 425gene 425 probes analyzed TumorNodeMetastasis Tumor Node Metastasis TNM (TNM staging score surgery prognosis alternations BAP PBRM SETD2 SETD CSFR CSF R NPM1 NPM EGFR RB 75 CCND2 CCND KRAS PTPN11 PTPN ERBB23 ERBB ERBB2 3 ERBB2/3 JAK2 JAK DiseaseFree Disease DFS. DFS . (DFS) SETD1 (PFS therapy Wildtype Wild type patient variants 7 PTPN1 ERBB2/ (DFS

RESUMEN El presente estudio se basa en varias pruebas de laboratorio montadas en una máquina de experimentos recientemente construida y modificada para este objetivo. Se realizaron las pruebas monofase y multifase de cizallamiento para determinar el punto de corte y el modelo de cizallamiento no saturado. De esta manera se obtuvieron los parámetros de cizallamiento de suelos no saturados bajo diferentes condiciones y los resultados coincidieron con las teorías evaluadas en esta materia. También se determinó la curva de corte para la succión matricial no lineal. Algunas ecuaciones de fuerza de cizallamiento también encajan en los resultados experimentales.

ABSTRACT The present study focuses on some experimental laboratory tests using a newly-constructed modified direct shear test apparatus. The single-stage and multistage direct shear tests were performed to determine the shear rate and test scheme of the unsaturated shear test. Shear strength parameters of unsaturated soil in different conditions are obtained, and the criteria indicate good agreement with standard theories of unsaturated soil. The nonlinear matric suction failure envelope is determined. Some shear strength equations are also fitted through the experimental results.

Abstract Background: Familial progressive hyper- and hypopigmentation (FPHH) is a rare genodermatosis that is characterized by diffuse hyper- and hypopigmented spots on the skin and mucous membranes. It is caused by a pathogenic mutation of the KITLG gene. Objectives: To investigate the clinical features and mutation of the KITLG gene in a Chinese family with FPHH. Methods: Histopathological and immunohistochemical analysis of lesions from the proband was performed. The KITLG gene was screened for the presence of mutations. Results: A Chinese family containing 14 individuals with FPHH was described, and the proband was a 5-year-old girl showing diffuse hyper- and hypopigmented lesions on her extremities and trunk. Histopathological and immunohistochemical staining for S100 and HMB45 of skin biopsy specimens from the hyperpigmented areas showed a striking increase in melanin throughout the epidermis, especially in the basal cell layer, and staining of hypopigmented area specimens displayed lower levels of melanin in the epidermis. Mutation analysis of the KITLG gene was performed, but no mutation was found. Study limitations: The new pathogenic gene was not found. Conclusion: A family with FPHH was described. Analysis revealed that its members did not have any mutations of the KITLG gene, which provided evidence for genetic heterogeneity of this genodermatosis.