ABSTRACT. The experiment was carried out to evaluate the quality of anhydrous milk fat (AMF) of cows and buffaloes supplemented with flaxseed oil (FO), soybean oil (SO), or their mixture (FSO). Lactating crossbred cows and buffaloes were fed with control diet or with one of three supplements: 2% FO, 2% SO, and 2% FSO according to a double 4 x 4 Latin Square Design. The diets with FO, SO, or FSO reduced saturated FA, mainly C4:0, C14:0 and C16:0, while increased the unsaturated FA C18:1 and C18:2 in milk from cows and buffaloes. Cholesterol content decreased in cow's AMF while increased in buffalo's AMF when a diet supplemented with FO, SO, or FSO. The diet with SO or FSO increased the content of vitamin E in AMF obtained from cows (25.06 and 17.89 mg 100 g-1) and buffaloes (28.48 and 30.32 mg 100 g-1) compared with the control diet (11.02 and 15.68 mg 100 g-1), respectively, which correlated positively with scavenging activity for DPPH• (r2 = 0.66) and ABTS• (r2 = 0.67) radicals. Solid fat content (SFC) was high for cow’s AMF, with 58.12-60.37% at 5°C compared to that of buffalo's AMF, with 52.37-56.98%, but was low for cow's AMF at >15°C. Finally, supplementing a diet with vegetable oils, particularly SO, improves the quality of AMF; increases USFA/SFA ratio, vitamin E content, and antioxidant activities. ABSTRACT (AMF FO , (FO) (SO) . (FSO) supplements 2 Design C40, C40 C C4 0, 0 C4:0 C140 C14 C14: C160, C160 C16 C16:0 C181 C18 1 C18: C182 cow s buffalos buffalo 25.06 2506 25 06 (25.0 1789 17 89 17.8 10 g1 g g-1 28.48 2848 28 48 (28.4 3032 30 32 30.3 11.02 1102 11 02 (11.0 1568 15 68 15.6 g1, respectively DPPH r2 r (r 0.66 066 66 ABTS 0.67 067 67 radicals SFC (SFC 58.1260.37% 58126037 58.12 60.37% 58 12 60 37 58.12-60.37 5C 5 52.3756.98%, 52375698 52.37 56.98%, 52 56 98 52.37-56.98% 15C >15°C Finally oils USFASFA USFA SFA ratio activities (FO (SO (FSO C4: C1 C16: 25.0 250 (25. 178 8 17. g- 28.4 284 (28. 303 3 30. 11.0 110 (11. 156 6 15. 0.6 1260 58.1260.37 5812603 5812 58.1 6037 60.37 58.12-60.3 3756 52.3756.98% 5237569 5237 52.3 5698 56.98% 9 52.37-56.98 25. (25 28. (28 11. (11 0. 126 58.1260.3 581260 581 58. 603 60.3 58.12-60. 375 52.3756.98 523756 523 52. 569 56.98 52.37-56.9 (2 (1 58.1260. 58126 60. 58.12-60 52.3756.9 52375 56.9 52.37-56. ( 58.1260 58.12-6 52.3756. 56. 52.37-56 58.126 58.12- 52.3756 52.37-5 52.375 52.37-

ABSTRACT. Produce and compare soft cheese with potential benefits of human health from Egyptian buffalo's and cow's milk was studied. Eight Egyptian lactating buffalos and cows were fed a total mixed ration supplemented with either 0% oil (CD), 2% flaxseed oil (DFO), 2% soybean oil (DSO), or 2% of their mixture (1:1, DFSO) according to a double 4 x 4 Latin Square design. Milk yield was similar between buffalo's diets but was higher in cows fed a DFO, DSO or DFSO resulting in 11.15, 8.21% or 8.97% increases compared with the control diet, respectively. Milk composition was not significantly affected in both buffalos and cows fed diets. The DFO, DSO or DFSO displayed decreased short-chain fatty acids, especially DSO and DFSO (3.73 and 3.33%, respectively) when compared to CD for buffalo milk (6.32%). The DSO and DFSO were more effective for increasing unsaturated fatty acids followed by the DFSO in buffalo's milk fat (42.31 and 41.90 %), whereas DFO and DFSO were more effective in cow's milk fat (39.67 and 39.84%), respectively. DFO, DSO or DFSO had no significant effect on the yield, composition and sensory properties of resultant soft cheese compared to the CD for both lactating cows and buffalos. During storage, a diet rich in unsaturated fatty acids enhances protein proteolysis and antioxidant activity of soft cheese during storage compared to the CD especially for soft cheese produced from buffalo's milk.