Abstract Ellagic acid (EA) is a phenolic biomolecule. For its biosynthesis, a source of ellagitannins is required, such as strawberries and yeasts, as precursors of the tannase and β-glucosidase enzymes responsible for hydrolysis of ellagitannins. Two experimental mixture designs were applied., varying the yeast concentration and the number of ellagitannins in the culture medium, evaluating the enzymatic activity and ellagic acid biosynthesis. Aiming to find the optimal compositions of the non-conventional yeasts assessed in the research to biosynthesize ellagic acid feasibly and efficiently using a response surface performing the statistical analysis in the StatGraphics® program for obtaining a higher yield and optimizing the ellagic acid synthesis process, the results indicate that the strains Candida parapsilosis ITM LB33 and Debaryomyces hansenii ISA 1510 have a positive effect on the synthesis of ellagic acid, since as its concentration increases in the mixture the concentration of ellagic acid in the medium also increases; on the other hand, the addition of Candida utilis ITM LB02 causes a negative effect, resulting in the compositions of 0.516876, 0.483124 and 2.58687E-9 respectively, for a treatment under the same conditions, an optimal value of ellagic acid production would be obtained. With an approximate value of 7.33036 mg/mL. EA (EA biomolecule biosynthesis required βglucosidase β glucosidase applied applied. nonconventional non conventional StatGraphics process LB LB3 151 hand LB0 0516876 0 516876 0.516876 0483124 483124 0.48312 2.58687E9 258687E9 E 2.58687E 9 2 58687E 2.58687E- respectively conditions obtained 733036 7 33036 7.3303 mgmL mg mL mg/mL 15 051687 51687 0.51687 048312 48312 0.4831 58687E9 258687E 73303 3303 7.330 1 05168 5168 0.5168 04831 4831 0.483 7330 330 7.33 0516 516 0.516 0483 483 0.48 733 33 7.3 051 51 0.51 048 48 0.4 73 3 7. 05 5 0.5 04 4 0.

Abstract Pomegranate (Punica granatum) contains secondary metabolites with antioxidant and bactericide activity; however, the study of the peel in the endemic varieties of Mexico has not been deepened. The polyphenols extraction of peel pomegranate endemic to the state of Michoacan, Mexico could be used in the formlulation of healthy food due contains antioxidant compounds or could be used like drugs due contains antibactericide compunds.In this work 3 varieties of pomegranate were analyzed; Wonderful, Apaseo and Tecozautla harvested in 2017 and 2018, carrying out a physicochemical characterization to establish the ripening, application of an experimental design of response surface for drying the peel and extracting polyphenols using two solvents (acetone and ethanol) by the Soxhlet method. As a result, the pomegranates were in the correct ripening, in the drying an optimal point of operation was found without affecting the metabolites (36 h at 55 °C) and in the extraction, the bactericide and antioxidant activity was evaluated observing that in the ketone extracts the best results were obtained in the Apaseo variety being; ABTS•+ technique of 150.78 ET mM/g, DPPH• 109.8 ET mM/g and 11.82 EAG mg/g in dry extract. For the bactericide activity measured by inhibition halos in S. aureus and E. coli it was had; 20.03 mm and 14.05 mm respectively for the Apaseo variety, which is why it is convenient to extract polyphenols under this method in peel of Mexican pomegranate varieties. Punica granatum however deepened Michoacan compundsIn compunds In analyzed Wonderful 201 2018 ripening acetone ethanol result 36 (3 5 °C C being ABTS ABTS• 15078 150 78 150.7 mMg mM g DPPH 1098 109 8 109. 1182 11 82 11.8 mgg mg S E had 2003 20 03 20.0 1405 14 05 14.0 ( 1507 15 7 150. 10 118 1 11. 200 2 0 20. 140 14.

Abstract The objective of this study was to evaluate the β-glucosidase activity in the non-conventional yeasts under cellulose, glucose and sucrose substrates. The participation of the enzyme β-glucosidase and its contribution to the enzymatic degradation of tannins is known. Within the classification of tannins are ellagitannins, molecules of gallic acid and ellagic acid, which are considered as nutraceutical compounds due to the properties that they present and that they can be used in the design of food and new drugs, synthesis of materials with antimicrobial capacity. The extracellular β-glucosidase activity was mainly presented in the Candida and Pichia strains, being the glucose and sucrose media the most capable for inducing the activity that showed maximum values with P. pastoris in glucose (0.1682±0.00 µmol/min mg protein), and C. utilis in cellulose (0.1129±0.1349 µmol/min mg of protein), and sucrose (0.0657±0.0214 µmol/min mg protein). Additionally, I. terricola and P. kluyvery stood out in a qualitative cellulose degradation approach measured by Congo red method (9.60±0.04 mm and 9.20±0.05 mm respectively). These indicate that P. pastoris and C. utilis have potential as β-glucosidase producers, especially when growing under complex carbon sources for biomass conversion, new biofuels production and polyphenol degradation with more manageable bioreactor process. βglucosidase β glucosidase nonconventional non conventional substrates known ellagitannins drugs capacity strains P 0.1682±0.00 01682000 0 1682 00 (0.1682±0.0 µmolmin µmol min protein, protein , protein) C 0.1129±0.1349 0112901349 1129 1349 (0.1129±0.134 0.0657±0.0214 0065700214 0657 0214 (0.0657±0.021 protein. . Additionally I 9.60±0.04 960004 9 60 04 (9.60±0.0 920005 20 05 9.20±0.0 respectively. respectively respectively) producers conversion process 0.1682±0.0 0168200 168 (0.1682±0. 0.1129±0.134 011290134 112 134 (0.1129±0.13 0.0657±0.021 006570021 065 021 (0.0657±0.02 9.60±0.0 96000 6 (9.60±0. 92000 2 9.20±0. 0.1682±0. 016820 16 (0.1682±0 0.1129±0.13 01129013 11 13 (0.1129±0.1 0.0657±0.02 00657002 06 02 (0.0657±0.0 9.60±0. 9600 (9.60±0 9200 9.20±0 0.1682±0 01682 1 (0.1682± 0.1129±0.1 0112901 (0.1129±0. 0.0657±0.0 0065700 (0.0657±0. 9.60±0 960 (9.60± 920 9.20± 0.1682± 0168 (0.1682 0.1129±0. 011290 (0.1129±0 0.0657±0. 006570 (0.0657±0 9.60± 96 (9.60 92 9.20 0.1682 016 (0.168 0.1129±0 01129 (0.1129± 0.0657±0 00657 (0.0657± 9.60 (9.6 9.2 0.168 01 (0.16 0.1129± 0112 (0.1129 0.0657± 0065 (0.0657 9.6 (9. 9. 0.16 (0.1 0.1129 011 (0.112 0.0657 006 (0.065 (9 0.1 (0. 0.112 (0.11 0.065 (0.06 ( 0. (0 0.11 0.06 (0.0 0.0

Abstract Mezcal is a traditional alcoholic beverage in Mexico obtained from fermentation of juices, coming from mature agave pineapples, and their subsequent distillation, Agave cupreata juice as natural sugar source is the commonly used. This study aims to investigate mezcal production from Agave cupreata juice by optimizing fermentation at flask level temperature; initial content sugar; pH and initial cell concentration, using single strains, factorial fractional design was applied and for fermentation at bioreactor level 2.5 Liters a Simplex centroid design was developed to determine the most appropriate yeast consortium for producing mezcal. The fermentation process was carried out for 52 h with varied input variables, and all the models showed significant p-values for interaction of variance (< 0.05). It was produced at an optimized temperature of 32.5 °C, initial quantity sugar of 14 °Brix, pH of 5.5 and stirring agitation of 150 rpm was found that it is possible to reach ethanol concentration levels of about 5.9, 5.2, 7.3 and 4.9% v/v for S. cerevisiae, C. lusitaniae, K. marxianus and Z. bailii, respectively. Therefore K. marxianus and Z. bailii strains reached the highest production of ethanol. Fermentation broths from the mixture design were distilled and rectified determining alcohol concentration; mixtures of K. marxianus and Z. bailii reached ethanol content in their distillates, about 42.82% v/v. The developed models could predict the quality of mezcal developed from Agave cupreata using a yeast consortium.

Abstract The pomegranate (Punica granatum) belong the Punicaceas family and is a plant of Iran that is characterized by the fruit that it give because is a big globular berry that inside have a lot of seeds wrapped in a juicy edible pulp covered for a gross peel with green, yellow and red tonalities depending of the ripening state and the varieties. The harvest and consumption of the fruit has gone increasing in Mexico due of the actual findings of phytochemical compounds whit antioxidant, anti-inflammatory and antiviral activity with application in products of food industry and pharmaceutical and it has even been observed microbicide activity versus fungus responsible of crop rot and pathogenic bacteria, nevertheless, in the treatment of the fruit the peel isn´t used and is discarded, so, in this project we want to describe on general way the fruit and the alternatives that has been worked actually in the exploration and application of phytochemical compounds like the polyphenols in whole the plant.

Abstract Chlorella vulgaris is a microalgae belonging to the order of the Chlorococcales, of the Oocytaceae family, of the genus Chlorella, which has a green colour due to the chloroplasts it contains. Its shape is spherical with a size that varies from 1 to 10 microns. These microalgae contain, in addition to chlorophyll, a significant amount of intracellular proteins, carbohydrates, lipids, vitamin C, β-carotenes and B vitamins (B1, B2, B6 and B12), which is why it is commonly used for the preparation of food supplements, as well as for the production of cosmetics, clinical treatments and even for the detoxification of heavy metals in wastewater. For this reason, the following review speaks from the morphology of the microalgae C. vulgaris to recent investigations regarding the primary and secondary metabolites. This research also provides an overview of the areas of opportunity for the development of new products and process improvements in order to increase the existing yields so far to optimize responses based on the desired products, the formulation of various growth media or the design of new photobioreactors which allow greater control of growth conditions and easy scaling for high productions at the industrial level that cover the current global needs.

Abstract The pomegranate (Punica granatum) is a bush with abundant foliage originating from Iran, it was brought to Mexico during the time of the conquest and whose main characteristic is the fruit it generates, which is a big, globose berry with thick, shiny skin of red, green or yellow colour depending on the specie of pomegranate and the ripening stage in which itis founding. Recent research has shown that pomegranate contains chemical compounds with antioxidant and bactericidal activities resulting from its secondary metabolism as what are mainly polyphenols, terpenes and alkaloids, which affect the specific concentration of the state of maturity of the product and where most of them, polyphenolic compounds, are found in large quantities at this stage, presenting antioxidant activity and bactericide. The present article has the objective to analyse the ripening stage of three varieties of mexican pomegranate (Wonderful, Apaseo and Tecozautla) through a physicochemical characterization for which the morphology (sizes, colours and hardness) was analyzed as the amount of total suspended solids, pH and titratable acidity in the juice of the product to finish with the determination of the concentration of polyphenolic compounds with possible antioxidant and bactericide activity.

Abstract Non-conventional yeasts represent an alternative for microorganisms that are capable to produce the enzyme Tannase (EC 3.1.1.20), which has many applications is the food and pharmaceutical industry as it stands out for its ability to produce ellagic acid out of the enzymatic hydrolysis of ellagitannins. Ellagic acid has been studied in the medical area for its action against various diseases, as is the case of cancer or heart problems. The investigation was conducted to evaluate seven non-conventional yeasts - Debaryomyces hansenii PYC ISA 1510, Debaryomyces hansenii PYC 2968, Candida parapsilosis, Candida utilis, Pichia pastoris, Pichia kluyveri, Issatchenkia terricola - as producers of the enzyme tannase. This was done in mediums of fermentation with tannic acid (1-3%) and YPD without tannic acid, to determine the activity of the enzyme by two spectrophotometric methods, the first being with rhodamine and the second with methyl gallate, to detect the presence of gallic acid as a product of the hydrolysis of tannic acid. The main results showed that the evaluated non-conventional yeasts all presented activity with the Tannase enzyme, obtaining better detection results with the rhodamine technique for the stability of the formed complex, which gives more precision of the obtained data.

Abstract Characterize the content of phytochemical compounds present in samples of extracts of strawberry (Fragaria sp.) and blackberry (Rubus sp.). The extraction of said compounds was carried out with solutions of polar solvents, such as acetone, ethanol and methanol, using an experimental design with three factors: state of maturity, resting time and temperature. The characterization of these fruits is the beginning of the utilization mainly of ellagitannins to produce ellagic acid. Total phytochemical content was determined by spectrophotometric techniques, for phenolic content it was used Folin-Ciocalteu, for flavonoids it was determined with aluminium chloride, the antioxidant activity was evaluated by the methods of DPPH and ABTS+, the bactericidal effect of the extracts was shown for strains of E. coli and S. aureus. The determination of ellagitannins was performed by spectrometry. The influence of the state of maturity on the response variable was evaluated; it was observed that concentration of phytochemicals increases proportionally to maturity stage. The antioxidant activity was evaluated, determining that extracts obtained exhibit 94% - 95% inhibition. The determination of ellagitannins, finding that the highest concentration of ellagitannins was found in a state of green maturity, for strawberries as well as blackberries.

Abstract Avocado represents one of the most nutritious and consumed foods in Mexico, and its export generates significant profits; because of its fatty acids content, the avocado oil was used as an inducer for the synthesis of extracellular lipases from the non-conventional yeast Kluyveromyces marxianus L-2029. Lipases are able to catalyze acyl glycerides, hydrolyzing them into fatty acids and glycerol; they have several applications in various industries. In order to determine the ideal initial avocado oil concentration in the culture medium for the yeast, the induction was carried out at different concentrations in incubation for 24 h. The yeast presented the greater extracellular lipase productivity at a 3.5% v/v avocado oil concentration, with a 3.47 μmol PNF/mg prot * min (U/mL) maximum activity. The enzymatic extract obtained at this condition had an optimum lipase activity temperature of 36 °C and pH 6. The pYJR10W lipase was detected in the enzymatic extract from avocado oil induction, and is therefore responsible for the extracellular lipase activity of K. marxianus.

En la industria alimenticia, los agentes microbicidas son usados para preservar la calidad y seguridad de los alimentos procesados. Los flavonoides encontrados en extractos cítricos han mostrado capacidad de inhibición del crecimiento celular de un gran grupo de microorganismos infecciosos, por lo tanto, éstos compuestos pueden ser útiles como agentes antivirales, antifúngicos y antibacteriales. Los flavonoides que se pueden encontrar principalmente en varias de las especies cítricas son hesperetina, hesperidina, luteolina, naringenina, naringina, narirutina, neohesperidina, nobiletina y tangeretina. A continuación se resumen los procesos utilizados recientemente para extraer, purificar y analizar los flavonoides principales en frutas cítricas. Ya optimizado el medio de cultivo se procedió a realizar una cinética confirmatoria con base a las condiciones encontradas pero evaluando el crecimiento celular por conteo en cámara de Neubauer y la producción de etanol mediante método enzimático (Procedimiento de ensayo K-ETOH, Megazyme) para confirmar los valores.

In the food industry, antimicrobial agents are used for preserving the quality and safety of processed food. Flavonoids found in citrus extracts inhibit cell growth of a large group of infectious microorganisms, therefore, these compounds may be useful as antiviral, antifungal and antibacterial agents. Hesperetin, hesperidin, luteolin, naringenin, naringin, narirutin, neohesperidin, nobiletin and tangeretin are some of the main flavonoids found in various citrus fruits. The processes used in recent years to extract, purify and analyze typical flavonoids from citrus species are reviewed.

En el presente trabajo, se efectuó una compilación de investigaciones realizadas sobre las diferentes etapas del proceso productivo del mezcal a partir de agave en algunas regiones productoras de México; con el propósito de explicar las problemáticas que enfrenta la cadena productiva agave-mezcal y las estrategias científicas que se han desarrollado para solucionarlas. Con ello se pretende lograr su resistencia y persistencia productiva en el mercado, elaborando un producto que cumpla con las características químicas establecidas por las normas oficiales mexicanas de producción y certificación de calidad, además de ofrecer posibles medidas de control en los procesos para estandarizar el producto y finalmente lograr etapas más eficientes en el proceso de producción.

In this paper, a compilation of investigations of the different stages in the production process of mezcal from agave in some producers regions of Mexico is carried out; to explain some of the problems faced by the productive chain agave-mezcal and scientific strategies that are being developed in the country to solve them. This is intended to achieve its resistance and productive persistence in the market, producing a product that meets the chemical characteristics established by the Mexican official standards of production and quality certification, in addition to establishing more measures of control processes to standardize the product and finally to achieve more efficient steps in the production process.

Introduction: nosocomial infections constitute an important problem related to the quality and efficiency of the health services. Objective: to characterize nosocomial infections at “Pepe Portilla” Provincial Children Hospital during 2010-2011. Material and method: an observational, descriptive, cross-sectional and applied research was conducted, which target group was comprised of 22652 patients discharged from the hospital and the sample included 516 patients. To collect the information, the records of the statistics department and the clinical histories were reviewed; where absolute frequencies and relative percentages plus chi square test up to 95% of certainty were used to complete its analysis. Results: respiratory tract was the most affected site, where negative hemocultures prevailed. Staphylococcus epidermidis, Pseudomonas sp and Acinetobacter germs were the most isolated, among others. Conclusions: nosocomial infections constitute a health problem in paediatrics, which requires the strengthening of prevention and control activities, emphasizing on its systemic surveillance.

Introducción: las infecciones nosocomiales constituyen un importante problema de salud relacionado con la calidad de la atención y la eficacia del trabajo. Objetivo: caracterizar las infecciones nosocomiales en el Hospital Pediátrico Provincial Docente "Pepe Portilla" durante el período 2010-2011. Material y método: se realizó una investigación observacional, descriptiva, transversal y aplicada. El universo estuvo conformado por los 22652 egresados y la muestra por los 516 casos. Para la obtención de la información se revisaron los registros del departamento de estadística y las historias clínicas, utilizándose para su análisis las frecuencias absolutas, relativas porcentuales y la prueba de X² al 95 % de certeza. Resultados: el sistema respiratorio es el sitio de mayor localización, donde predominaron los hemocultivos negativos. Los gérmenes más aislados fueron: el Staphylococcus epidermidis, la Pseudomonas sp y el Acinetobacter, entre otros. Conclusiones: las infecciones nosocomiales en pediatría constituyen una problema de salud que requiere el fortalecimiento de las actividades de prevención y control, con especial énfasis en su vigilancia sistemática.

Biotechnological processes with lignocellulose materials are promising technologies for the production of chemicals and energy involving the same main production stages: hydrolysis of the hemicellulose and the cellulose to monomeric sugars, fermentation, recovery of the product, and concentration. We studied the effect of different treatments on the release of reducing sugars (RS) from seeds. Three factors were tested: temperature from 86 to 130.2 °C; acid concentration, 0.32 to 3.68% (v/v); and exposure time, from 13.2 to 40 min. Two acids were tested. Temperature and time were the factors that had more effect on hydrolysis, whereas no interactions between factors had a significant effect on the release of RS. The best conditions for RS release were for both acids (2% v/v), 130.2 °C, and 30 min, with a concentration of about 110 g/L. Results suggest tamarind seed is a feasible option for xylitol production.

Los procesos biotecnológicos con materiales lignocelulósicos son tecnologías prometedoras para la producción de químicos y energía involucran las mismas etapas de producción: Hidrólisis de la hemicelulosa y celulosa a azúcares monoméricos, fermentación, recuperación de productos y concentración. Se estudió el efecto de diferentes tratamientos en la liberación de azúcares reductores (RS) de las semillas. Se probaron tres factores: temperatura (86-130 °C), concentración de ácido (0.32-3.68%) y tiempo de exposición (13.2-40 min). Se probaron dos ácidos. La temperatura y el tiempo fueron los factores que tuvieron más efecto significativo en la liberación de RS. Las mejores condiciones para la liberación de los RS fueron 130.2 °C, 2% de ácido v/v, and 30 min, alcanzando un valor de aproximadamente 110 g/L. Los resultados sugieren que las semillas de tamarindo son una opción viable para la producción de xilitol.

OBJECTIVE: To estimate the prevalence of antibodies against Trypanosoma cruzi in blood donors from Hospital General Regional (HGRO) of the Mexican Institute of Social Security (IMSS per its abbreviation in Spanish). MATERIAL AND METHODS: Between October 2001 and January 2002, blood samples were collected from voluntary donors at the blood bank of the Hospital General Regional of IMSS in Orizaba; Veracruz, Mexico. The samples were assayed for anti-T. cruzi by ELISA, Western blot and IFI, using a recombinant protein (MBP::Hsp70), and crude extract from epimastigotes. RESULTS: A total of 420 blood donors were studied; two of them were seropositive for ELISA, Western blot and IFI, with a seroprevalence of 0.48%. CONCLUSIONS: Some blood donors at the HGRO hospital were seropositive for T. cruzi, showing the risk of contamination by blood transfusion. Routine serologic screening with highly sensitive and specific immunological techniques are needed.

OBJETIVO: Determinar la prevalencia de anticuerpos contra Trypanosoma cruzi en donadores del Hospital General Regional del Instituto Mexicano del Seguro Social (IMSS) en la ciudad de Orizaba, Veracruz. MATERIAL Y MÉTODOS: Se examinaron muestras de donadores del banco de sangre del Hospital General Regional (HGRO) del IMSS para la búsqueda de antiT. cruzi por ELISA, Western blot e IFI, utilizando una proteína recombinante (MBP::Hsp70) y un extracto crudo de epimastigotes. Las muestras fueron obtenidas entre los meses de octubre de 2001 a enero de 2002. RESULTADOS: Los 420 donadores de sangre analizados fueron seronegativos para HBV, HCV, BrA, VDRL y HIV. Después del tamizaje de los 420 donadores, se identificaron dos individuos seropositivos por las pruebas de ELISA, Western blot e IFI, con una seroprevalencia de 0.48%. CONCLUSIONES: En este estudio se muestran evidencias de seropositividad para T. cruzi en donadores de sangre del HGRO, lo que sugiere la existencia de riesgo de contaminación por transfusión sanguínea. Por tal motivo, es necesario aplicar programas para el tamizaje serológico a través de técnicas inmunológicas con alta sensibilidad y especificidad.