Brazil is home to a rich biodiversity of native edible fruits renowned for their health-promoting compounds and flavors. Among these, Eugenia uniflora L. (Brazilian pitanga) is particularly notable. The fruit varies in color from yellow to purple and is prized for its intense aroma and versatile taste profile. However, information about the compounds responsible for their nutritional and sensory potential is still limited. This study analyzed volatile compounds and metabolites in yellow, red and purple pitanga fruits (Eugenia uniflora) at various ripening stages, exploiting a metabolomics approach. Yellow pitanga had 30 volatile compounds, with esters (40%), terpenes (26.67%) and aldehydes (16.67%) constituting 83.34% of identified volatile compounds. Red pitanga featured 26 volatile compounds, primarily terpenes and esters (each 30.77%) and aldehydes (15.38%), making up 76.92%. Purple pitanga contained 39 volatile compounds, with terpenes and esters (each 35.90%) and aldehydes (17.95%), totaling 89.75% of identified volatile compounds. Across all pitanga types, 124 metabolites were identified, including saturated fatty acids (17.74%), terpenes (17.74%) and sugars (16.93%), constituting 52.41% of identified metabolites. Future studies should provide nutritional information, consumption methods and industrial applications for pitanga fruits, focusing on sensory analysis, health benefits, preservation and breeding to enhance bioactive compounds and flavor. healthpromoting promoting flavors these L Brazilian notable profile However limited stages approach 3 40%, 40 40% , (40%) 26.67% 2667 67 (26.67% 16.67% 1667 16 (16.67% 8334 83 34 83.34 2 each 30.77% 3077 77 15.38%, 1538 15.38% 15 38 (15.38%) 7692 76 92 76.92% 35.90% 3590 35 90 17.95%, 1795 17.95% 17 95 (17.95%) 8975 89 75 89.75 types 12 17.74%, 1774 17.74% 74 (17.74% 16.93%, 1693 16.93% 93 (16.93%) 5241 52 41 52.41 analysis benefits flavor 4 (40% 26.67 266 6 (26.67 16.67 166 1 (16.67 833 8 83.3 30.77 307 7 153 15.38 (15.38% 769 9 76.92 35.90 359 179 17.95 (17.95% 897 89.7 177 17.74 (17.74 169 16.93 (16.93% 524 5 52.4 (40 26.6 (26.6 16.6 (16.6 83. 30.7 15.3 (15.38 76.9 35.9 17.9 (17.95 89. 17.7 (17.7 16.9 (16.93 52. (4 26. (26. 16. (16. 30. 15. (15.3 76. 35. 17. (17.9 (17. (16.9 ( (26 (16 (15. (17 (2 (1 (15

Abstract ‘BRS Sarau’ is a highly productive peach cultivar widely adapted to the Southern and Southeast region of Brazil. It produces fruits of excellent flavor and shape, with attractive skin and flesh color, and its harvest starts near the Christmas holidays, when the demand for peaches is high. BRS Sarau Brazil shape color holidays high

Resumo Em 1963, começou na Estação Experimental de Pelotas, hoje Embrapa Clima Temperado, um programa de melhoramento de pessegueiro cujo objetivo fundamental era desenvolver cultivares adaptadas a condições de inverno ameno. Inicialmente, a prioridade era a obtenção de cultivares produtoras de frutos para enlatamento, mas, ao longo dos anos, cultivares para o mercado fresco adquiriram a mesma importância. Este artigo analisa dados de 84 cultivares, tipo mesa, desenvolvidos em Pelotas, de 1964 a 2017, focando nos seguintes parâmetros: época de maturação, período de desenvolvimento do fruto, massa média dos frutos, número de frutos por planta, produção por planta e conteúdo de sólidos solúveis das frutas. Inicialmente, os dados foram tabulados, divididos em dois períodos (1964-1984 e 1985-2017), sendo então realizada a análise estatística descritiva, seguida por uma análise de modelos mistos e estimativa do progresso genético via meta-análise. Os resultados mostraram uma redução no período de desenvolvimento dos frutos das cultivares tipo mesa, pertencentes a esse programa. Houve ainda uma ampliação do período de colheita (do fim de setembro ou início de outubro até janeiro), com uma leve tendência à maior precocidade. Verificou-se um ganho genético para produção por planta, correspondendo a 1.17 e 2.25% ao ano, para os períodos de 1964- 1984 e 1985-2017, respectivamente. 1963 Pelotas Temperado ameno Inicialmente enlatamento mas anos importância 8 mesa 196 2017 parâmetros maturação fruto frutas tabulados 19641984 (1964-198 19852017, 19852017 1985 , 1985-2017) descritiva metaanálise. metaanálise meta análise. meta-análise janeiro, janeiro janeiro) precocidade Verificouse Verificou se 117 1 17 1.1 225 2 25 2.25 ano 198 1985-2017 respectivamente 19 201 1964198 (1964-19 1985201 11 1. 22 2.2 1985-201 20 196419 (1964-1 198520 2. 1985-20 19641 (1964- 19852 1985-2 (1964 1985- (196 (19 (1 (

Abstract A peach breeding program started in 1963, at the Experiment Station of Pelotas, nowadays Embrapa Clima Temperado, whose primary aim was developing cultivars adapted to mild winter conditions. Its first priority was to obtain cultivars of canning type fruits, and over the years, the fresh market cultivars acquired equal importance. This article analyzes the data of 84 fresh market cultivars, obtained in Pelotas, from 1964 to 2017, focusing on the following parameters: time of ripening, fruit development period, average fruit mass, number of fruits per tree, productivity per plant and total soluble solids. First, the data were tabulated, divided into two periods (1964-1984 and 1985-2017), and the descriptive statistical analysis was performed, followed by an analysis via mixed models and estimates of genetic progress via meta-analysis. The main results revealed a reduced fruit development period of the fresh market peaches belonging to that program. It was also observed a significant spread of the time of ripening (end of September or beginning of October until January), with a slight tendency for earliness. Genetic gain was observed for yield corresponding to 1.17 and 2.25% per year, for 1964-1984 and 1985-2017, respectively. 1963 Pelotas Temperado conditions years importance 8 196 2017 parameters mass tree solids First tabulated 19641984 1984 (1964-198 19852017, 19852017 1985 , 1985-2017) performed metaanalysis. metaanalysis meta analysis. meta-analysis end January, January January) earliness 117 1 17 1.1 225 2 25 2.25 year 1964-198 1985-2017 respectively 19 201 1964198 198 (1964-19 1985201 11 1. 22 2.2 1964-19 1985-201 20 196419 (1964-1 198520 2. 1964-1 1985-20 19641 (1964- 19852 1964- 1985-2 (1964 1985- (196 (19 (1 (

Abstract Brown rot and blossom blight caused by fungi of the genus Monilinia are the most important peach diseases. The increased concern with the environment and the health of workers and consumers, as well as the emergence of fungus isolates resistant to the main fungicide molecules favor control strategies such as genetic resistance. The objective of this study was to adjust a phenotyping protocol for evaluation of resistance/susceptibility to blossom blight in peach, as well as to quantify the antioxidant compounds present in the petals of these flowers and their correlation with the disease incidence and severity. The experiment was arranged in a randomized complete block split-split plot design, the plot being four concentrations of Monilinia fructicola conidia; the subplot two phenological flower stage; and the sub-subplot four peach genotypes. The quantification of antioxidant compounds and their correlation with susceptibility to blossom blight was performed in the four genotypes analyzed. Phenotyping was more efficient when concentrations between 400 and 4,000 conidia mL-1 were used, regardless of phenological flower stage. The phenolic compounds, anthocyanins and antioxidant activity are positively correlated among them, and negatively correlated with the blossom blight incidence and severity. In order to estimate the blossom blight susceptibility, it is recommended to use flowers at the pink or bloom stage, inoculum equivalent to 20-200 conidia per flower, and perform the evaluation at 96 hours after inoculation. This study suggests that more intense pink flowers have a higher content of antioxidant compounds and less blossom blight susceptibility. diseases consumers resistance resistancesusceptibility severity splitsplit split design stage subsubplot sub analyzed 40 4000 4 000 4,00 mL1 mL 1 mL- used them 20200 20 200 20-20 9 inoculation 00 4,0 2020 2 20-2 0 4, 202 20-

ABSTRACT The adaptation of temperate fruit crops is a challenge being increased by the global warming. Chilling requirement is a key factor for adaptation. The objective of this study was to estimate the chilling requirement of peach cultivars BRS Bonão, Esmeralda, Granada and Eragil, using the Tabuenca test. Chilling accumulation was computed using four different chilling hour (≤ 7.2 ºC and ≤ 11 ºC) models; and chill units using the Low Chill model and the Taiwan model. The fresh bud weight and bud water contents were also evaluated. The Tabuenca test (based on differences in bud´s dry weight) showed a fairly good efficiency for estimating the end of dormancy in peach. However, under mild winter conditions, it is better to use fresh bud weights. Either one of three chilling accumulation computation models (temperature ≤ 7.2 °C, ≤ 11°C, or Taiwan model) is suitable to classify comparatively different cultivars, but none is accurate enough to conclude on the adaptation of a given cultivar to a specific site. Using hours of temperatures ≤ 11 ºC: ‘BRS Bonão’ needed around 180 hours for dormancy release; ‘Esmeralda’ around 250 hours; ‘Granada’ between 300 and 400 hours, and ‘Eragil’ more than 500 hours.

ABSTRACT: Strawberry guava (Psidium cattleyanumm Sabine) is a species native to southern Brazil which exhibits significant potential for economic exploitation. The objective of this study was to evaluate the growth response of strawberry guava subjected to liming and phosphate fertilizer in pre-planting. The treatments consisted of four lime levels: 0; 33.3; 66.6 and 100 % of the dose needed to increase the pH in water to 6.0, and four phosphorus (P) doses: 0; 83.3; 166.6 and 250 kg ha–1 of P2O5. Plant growth parameters were evaluated for three years. In the years 2018 and 2019, the mineral composition of leaves, photosynthethic pigment indices and mycorrhizal colonization on strawberry guava roots were measured. Liming and phosphate fertilizer altered neither plant growth nor the photosynthetic pigment indices and there was no interaction between the factors studied and the remaining variables evaluated. However, liming promoted increases in the Ca and Mg shoot content, and the phosphate fertilizer increased the levels of shoot P in the third year of growth. Mycorrhizal colonization was stronger when the P soil levels were low, which could explain the absence of response to phosphate fertilization on plant growth for the first three years after planting.

Abstract The Embrapa peach breeding program provides a processing peach cultivar, 'BRS Jaspe'. Over the years, the yield of this cultivar was stable and its fruits can be harvested by mid-November and have a good size and taste. It may represent an alternative to cultivar Granada, with a similar maturation period.

Abstract One of the reasons for the low market demand of blackberries in Brazil is the acid taste of the fresh fruit. The fruits of the newly released cultivar BRS Cainguá taste better and have a very attractive shape.

Resumo O pessegueiro foi introduzido no Brasil no século XVI; mas, apesar disso, o primeiro programa de melhoramento só teve início no País, no fim da década de 1940. Mais tarde, outros programas foram iniciados, mas os maiores e que mais contribuições deram à cultura do pessegueiro foram o do Instituto Agronômico de Campinas e o da Embrapa Clima Temperado. Atualmente, o pessegueiro é cultivado não apenas nas áreas mais frias do Sul do Brasil, mas também nas áreas subtropicais e tropicais de altitude, graças às dezenas de cultivares lançadas por estes programas de melhoramento genético. A produção de pêssego brasileira destina-se ao mercado interno, e no maior centro consumidor do País, São Paulo, a preferência é por pêssegos com polpa branca, doces, com baixa acidez e aparência atrativa. A cultivar BRS Serenata está sendo lançada com o objetivo de preencher a lacuna de pêssegos de polpa branca, entre a estação de ‘BRS Kampai’ e ‘BRS Fascínio’.

Abstract Even though the peach tree was introduced in Brazil during the XVI century, it was only on the 1940’s that the first peach breeding program started in the country. Later on, other programs started being, until now, the largest ones, and the programs that gave more significant contributions to the peach crop development those from the Instituto Agronômico de Campinas and from Embrapa Temperate Agriculture. Presently, peach is cultivated not only in the colder areas of the South but also in the subtropics and high altitude areas of the tropics, due to dozens of cultivars released by these breeding programs. Peaches are sold entirely in the domestic market and in the largest consumers market, State of São Paulo, the preference is for white sweet flesh and low acid peaches with attractive appearance. The ‘BRS Serenata’ cultivar was released to fulfill a gap of white flesh peach between the seasons of ‘BRS Kampai’ and ‘BRS Fascínio’.

ABSTRACT. Because of climatic changes, the cultivation of temperate climate plants such as peach in subtropical climates has become a challenge. In these areas, temperatures exceeding 25°C often occur during the pre-flowering and flowering phases. The high temperature causes damages by acting during the early stages of pollen-pistil interaction processes. The objective of this work was to evaluate the stigmatic receptivity of peach flowers at 18°C and 30°C. The pollen adherence was evaluated as well as the germination and presence of pollen tubes in the transmitting tissue of the style. The genotypes responded differently to temperature. ‘Granada,’ ‘Diamante’, and ‘Sensação’ had a stigmatic receptivity that was less affected when flowers were exposed to the higher temperature. Most genotypes showed a reduction in the number of pistils with pollen tubes growing in the style, particularly when pollination was delayed.

Abstract ‘BRS Citrino’ is a highly productive canning peach that has a round to conic shape. The flesh has a sweet acid flavor with a slight bitterness, giving a good quality product when canned. It is adapted to southern Rio Grande do Sul, where the production of processed peaches is concentrated.

Resumo O Brasil apresenta grande variedade de frutas nativas que têm despertado interesse comercial, a exemplo do araçá. Nesse sentido, muitas pesquisas têm buscado alternativas que permitam a disponibilidade do fruto pós-colheita, considerando que a perecibilidade é um fator limitante para sua comercialização. O processo de congelamento é uma alternativa importante no sentido de disponibilizar esses frutos por mais tempo no mercado. O uso de ácido L-ascórbico é um potente agente protetivo na estabilidade de compostos bioativos em polpas de frutas. O estudo teve como objetivo avaliar compostos potencialmente bioativos, presentes em polpas de araçás-vermelho e amarelo adicionadas de ácido L-ascórbico (1 % m/m) e verificar sua ação na retenção destes compostos em sua atividade antioxidante durante o armazenamento congelado (-20 ± 2ºC). Nas polpas, foram analisados: atividade antioxidante pela captura dos radicais DPPH° e ABTS°, fenóis, antocianinas e carotenoides totais. O ácido L-ascórbico influencia positivamente na retenção dos compostos bioativos (fenóis totais, antocianinas totais e carotenoides totais) em polpas de araçás-vermelho e amarelo, durante 120 dias de armazenamento congelado (-20 ± 2ºC).

Abstract Brazil presents a great variety of native fruits, which have aroused commercial interest, such as the strawberry guava. In this sense, many researches have looked for alternatives that allow postharvest availability of fruits, considering that perishability is a limiting factor for its commercialization. The freezing process is an important alternative in the sense of making these fruits available for longer on the market. The use of L-ascorbic acid is a potent protective agent in the stability of bioactive compounds in fruit pulps. The objective of this study was to evaluate potentially bioactive compounds present in red and yellow strawberry guava pulps added with L-ascorbic acid (1% m / m) and verify its effect on retention of these compounds and in its antioxidant activity during the frozen storage (-20 ± 2ºC). In the pulps were analyzed: antioxidant activity by the capture of the DPPH° and ABTS° radicals, phenols, anthocyanins and total carotenoids. L-ascorbic acid positively influences the retention of bioactive compounds (total phenols, total anthocyanins and total carotenoids) in red and yellow strawberry guava pulps during 120 days of frozen storage (-20 ± 2 ºC).

RESUMO Objetivou-se elaborar geleias de araçá vermelho do tipo convencional (com adição de sacarose) e sem adição de açúcares e avaliar a composição físico-química e teores de compostos bioativos presentes nas mesmas. Foram elaboradas quatro formulações de geleias: convencional com adição de sacarose (F1); com adição de aspartame (F2); com adição de sacarina e ciclamato (F3); com adição de acessulfame e sucralose (F4). Foram realizadas análises físico-químicas de pH, acidez total titulável, sólidos solúveis totais, cinzas, proteínas, lipídios, umidade, carboidratos, valor calórico, luminosidade, tonalidade de cor, fenóis totais, antocianinas totais, carotenoides totais, ácido ascórbico e atividade antioxidante pela captura dos radicais DPPH e ABTS. As geleias convencional e sem adição de açúcares não diferiram para fenóis totais, antocianinas totais e ácido ascórbico. Contudo, o processamento exerceu influência significativa (p=0,05) sobre carotenóides totais e atividade antioxidante. É viável a utilização de araçá vermelho para elaboração de geleias convencional e sem adição de açúcar. Os produtos, contudo, apresentam diferença significativa quanto aos teores de carotenóides, sendo os maiores teores destes e maior atividade antioxidante nas geleias processadas sem adição de açúcares.

ABSTRACT This study aimed to prepare jellies of conventional type of red strawberry guava (with added sucrose) and no added sugar and evaluate the physical and chemical composition and content of bioactive compounds in them. Four jellies formulations were prepared: conventional with addition of sucrose (F1), aspartame (F2), saccharin and cyclamate (F3), acesulfame and sucralose (F4). Physicochemical analysis of pH were carried out, as well as analysis of titratable acidity, total soluble solids, ashes, proteins, lipids, moisture, carbohydrates, calories, lightness, color tone, total phenols, anthocyanins, carotenoids, ascorbic acid and antioxidant activity, by the capture of DPPH and ABTS radicals. Conventional and no added sugars jellies did not differ for total phenols, total anthocyanins and ascorbic acid. However, processing exerted significant influence (p=0.05) on total carotenoids and antioxidant activity. It is feasible to use red strawberry guava for the preparation of conventional and no added sugar jellies. The products, however, show a significant difference in carotenoids content, with the highest content of these and higher antioxidant activity in processed jellies without sugars addition.

RESUMO Pêssegos tipo platicarpa tem alcançado ótimos preços devido à boa aceitação no mercado. Entretanto, a quase totalidade das cultivares lançadas são bastante exigentes em frio hibernal e não se adaptam às condições brasileiras, exceção representada por pequenas áreas de microclimas, com elevada altitude. A cultivar BRS Mandinho é a primeira cultivar da variedade botânica platicarpa lançada no Brasil e adapta-se a condições subtropicais. Suas plantas são vigorosas e a floração, no Rio Grande do Sul, ocorre na segunda quinzena de Julho. As frutas são doces e amadurecem em novembro e apresentam massa média de 55,3 g.

ABSTRACT In recent years, platycarpa peaches have reached high prices due to their good acceptance in the international market. However, the great majority of the released varieties are high chill and do not adapt to Brazilian conditions except on a few microclimes of high altitudes. The cultivar BRS Mandinho is the first peach cultivar of the botanical variety platicarpa, released in Brazil and adapted to subtropical conditions. The plants are vigorous and, in the state of Rio Grande do Sul, the blooming occurs on the second half of July. The fruits are sweet and ripe in November with an average weight of 55.3 g.

Este trabalho teve como objetivo avaliar o uso da enxertia de garfagem em fenda cheia na propagação vegetativa de diferentes genótipos de pitangueira. Foram utilizados sete genótipos do Banco Ativo de Germoplasma de fruteiras nativas do Sul do Brasil, mantidos na Embrapa Clima Temperado, em Pelotas-RS, dos quais foram coletados garfos (estacas semilenhosas), com aproximadamente 5 cm de comprimento. Utilizou-se como porta-enxertos de plantas de pitangueira oriundas de sementes. O diâmetro médio no ponto de enxertia foi de 2,5 mm. A enxertia foi realizada em setembro de 2006. O delineamento experimental foi o inteiramente casualizado, com quatro repetições, sendo a unidade experimental constituída por 10 plantas. Foram avaliadas as percentagens de brotação e de pegamento dos enxertos. Os percentuais de estacas brotadas variaram entre 45,0% e 95,0%, enquanto o pegamento dos enxertos variou entre 40,0% e 87,5%. Percentuais acima de 65,0% foram obtidos para as seleções "Pit 75", "Pit 61" e "Pit 137", para ambos os parâmetros avaliados, brotação e pegamento dos enxertos. Existe diferença entre genótipos desta espécie quanto à capacidade de pegamento na enxertia por garfagem no topo em fenda cheia. Este tipo de enxertia é apropriado para a propagação vegetativa da pitangueira.

This work had the objective of testing cleft grafting as a way to asexually propagate different genotypes of Surinam cherry. Bud wood of around 5 cm length were collected from seven genotypes of the Germplasm Bank of Embrapa Clima Temperado, Pelotas, RS. Seedling plants of Surinam cherry seedlings were used as rootstocks. The average diameter at the grafting point was 2.5 mm. Grafting was done on September, 2006. The experiment was carried out as a completely randomized design with four replications and 10 plants per plot. Evaluations were based on percentage of obtained plants in relation to the total number of grafts and percentage of grafts with leaf burst. Percentages of grafts with leaf break were between 45.0% and 95% whereas percentages of grafting success in actually obtained the desirable clones were between 40.0% to 87.5%. Percentages above 65.0% for evaluated parameters, leafing and grafting success were obtained in selections "Pit 75", "Pit 61" and "Pit 137". There were differences among genotypes for percentage of cleft grafting success in Surinam cherry propagation. This type of grafting is recommended for Surinam cherry vegetative propagation.