Abstract The structure of urban secondary forests and their potential for meliponiculture in the eastern Amazon was studied in the municipality of Belterra, Pará. The sampling was carried out in 25 plots of 40 m2 in two areas (0.1 ha) of secondary forests (SF), in which three classes of DBH values were considered (C1:1 cm ≤ DBH ≤ 5 cm; C2: 5 cm < DBH ≤ 10 cm and C3: DBH ≥ 10 cm). a total of 230 individuals were registered in SF I, comprising 66 species and 27 botanical families and 185 individuals in SF II, comprising 43 species distributed in 24 botanical families. The highest richness and abundance were verified for C1, followed by C2 in both areas. In SF I, the basal area values (G=1.509) were higher than in SF II, while the total volume (V=20.21) was higher than SF I, the mean height for C3 was higher in SF II, and C1 and C2 were higher in SF I. The Shannon-Wiener diversity index was higher in SF I (H = 3.722) than in SF II (H=3.197). It was observed that 75% species have bee pollination systems (mellitophilous flora) and are thus considered sources of honey resources. Belterra Pará 2 4 m 0.1 01 0 1 (0. ha SF, , (SF) C11 C (C1: cm. . cm) 23 6 18 G=1.509 G1509 G 509 (G=1.509 V=20.21 V2021 V 20 21 (V=20.21 ShannonWiener Shannon Wiener H 3.722 3722 3 722 H=3.197. H3197 H=3.197 197 (H=3.197) 75 mellitophilous flora resources 0. (0 (SF (C1 G=1.50 G150 50 (G=1.50 V=20.2 V202 (V=20.2 3.72 372 72 H319 H=3.19 19 (H=3.197 7 ( (C G=1.5 G15 (G=1.5 V=20. V20 (V=20. 3.7 37 H31 H=3.1 (H=3.19 G=1. G1 (G=1. V=20 V2 (V=20 3. H3 H=3. (H=3.1 G=1 (G=1 V=2 (V=2 H=3 (H=3. G= (G= V= (V= H= (H=3 (G (V (H=

Resumo A andiroba (Carapa) representa uma importante fonte de recursos naturais. O objetivo deste trabalho foi estudar a morfologia polínica das três espécies de Carapa (Meliaceae) registradas na Amazônia brasileira. Os grãos de pólen foram obtidos de botões florais, flores pistiladas e estaminadas de Carapa guianensis, C. surinamensis e C. vasquezii. A fim de explorar as implicações taxonômicas do estudo de pólen para estas espécies de Carapa, o material foi preparado de acordo com o método de acetólise láctico, permitindo a utilização de estudos de microscopia de luz. Além disso, o material não-acetolisado foi utilizado no estudo da microscopia eletrônica de varredura. O estudo mostra que as três espécies de Carapa apresentam pequenas variações na morfologia polínica. Comparada com as outras espécies deste estudo, Carapa vasquezii têm valores mais elevados para o comprimento e largura da endoabertura, entretanto, tem valores inferiores para diâmetro polar, equatorial, diâmetro equatorial em vista polar e lado do apocolpo.

Abstract The crabwood (Carapa) represents an important source of natural resources. The objective of this work was to study the pollen morphology of the three species of Carapa (Meliaceae) registered in the Brazilian Amazon. The pollen grains were obtained from floral buds, pistillate and staminate flowers of Carapa guianensis, C. surinamensis and C. vasquezii. In order to explore the taxonomic implications of the pollen study for these species of Carapa, lactic acetolysis method was used for light microscopy study. Furthermore, the non-acetolyzed material was used in the study of the scanning electron microscopy. It was found that the three Carapa species present little variations in pollen morphology. Compared to the other species this study, Carapa vasquezii has higher values for the length and width of endoaperture; however, it has lower values for polar and equatorial diameter, equatorial diameter in polar view and apocolpium side.

We applied an "adapted" protocol for collecting and processing pollen grains in the pollen analysis of the post-emergence residue of Melipona (Melikerria) interrupta Latreille. The study was conducted at the Sant'Ana honey farm, located on the banks of the Solimões River, in the municipality of Manacapuru, in the state of Amazonas, Brazil, where a colony was monitored in October and November 2010. From that colony, 10 samples of post-emergence residue were collected. Unlike in the acetolysis method, there was no need to expose pollen grains to an acidic medium, because pollen loses its content during the larval digestive process. We identified 32 pollen types, from 19 botanical families, plus three undetermined pollen types. The most representative family was Fabaceae (Mimosoideae), with eight pollen types, Mimosa guilandinae being the most common species. Only the pollen of Miconia (Melastomataceae), with 74.10%, was classified as a common pollen. We also found that the pollen of Mimosa pudica (Fabaceae: Mimosoideae) retained its content, indicating that not all resources furnished by workers are utilized by the larvae. The protocol applied here, despite omitting the acetolysis process, was efficient, providing full details of pollen contained in post-emergence residue.

Foi realizado um estudo da morfologia polínica de espécies Gentianaceae Juss. ocorrentes na Reserva Florestal Adolpho Ducke, Manaus, Brasil. Os grãos de pólen das espécies estudadas variaram morfologicamente quanto à unidade polínica (tétrades ou mônades), ao tamanho (pequenos, médios e grandes), à forma (oblata, suboblata, oblata-esferoidal, esférica, prolata-esferoidal e prolata, à polaridade (heteropolar, isopolar ou subisopolar), à simetria (bilateral ou radial) e ao número e tipo de aberturas (3-colporados e 1-3 porados). Quanto à ornamentação, foram reconhecidos grãos de pólen com sexina psilada nas espécies de Voyria, microrreticulada em Voyriella parviflora e reticulada heterobrocada em Tachia grandiflora e Irlbachia alata. A exina em I. alata mostrou grande variação na forma e tamanho dos lumens além da formação de clavas de tamanhos variados em áreas de mesocolpo e apocolpo. Em Tachia grandiflora, além de colpos longos, a exina heterobrocada apresentou um aumento do lúmen, em regiões do mesocolpo, diminuindo, consideravelmente, nas áreas do apocolpo. Nas espécies de Voyria, os grãos de pólen mostraram diferença quanto à forma, polaridade e ao número de aberturas, ocorrendo esferoidais, isopolares e 3-porados em Voyria spruceana; prolata/oval, heteropolares e 1-porados em Voyria tenella e oblato/elíptico (reniforme), heteropolares e 2-(3) porados em Voyria aphylla e Voyria caerulea. A variação na morfologia polínica e características exclusivas do gênero têm sugerido que Voyria seja tratada como um táxon único dentro de Gentianaceae.

A pollen morphology study of the Gentianaceae Juss. of Reserva Florestal Adolpho Ducke (Manaus, Brazil) was performed. The pollen grains of the studied species varied morphologically in their pollen units (tetrads or monads), size (small, medium and large), shape (oblate, suboblate, oblate spheroidal, spheroidal, prolate spheroidal and prolate), polarity (heteropolar, isopolar or subisopolar), symmetry (bilateral or radial) and number and type of aperture (3-colporate and 1-3porate). In relation to ornamentation, pollen grains had a sexine that was psilate in the Voyria species, microreticulate in Voyriella parviflora and reticulate heterobrochate in Tachia grandiflora and Irlbachia alata. The exine in I. alata showed great variability in the size and shape of the lumina, in addition to clavae of various sizes in the mesocolpium and apocolpium areas. In Tachia grandiflora, besides to long colpi, the heterobrochate exine demonstrated an increase in the lumina in the mesocolpium areas that decreased considerably in the apocolpium areas. In the species of Voyria, the pollen grains showed differences in shape, polarity and the number of apertures, which were spherical, isopolar and 3-porate in Voyria spruceana; prolate/oval, heteropolar and 1-porate in Voyria tenella; and oblate/elliptical (reniform), heteropolar and 2(3)-porate in Voyria aphylla and Voyria caerulea. The variations in pollen morphology and the peculiar characteristics of the genus suggest that Voyria should be treated as a unique taxon within Gentianaceae.