Abstract The present study aimed to investigate the socioeconomic and obstetric characteristics of adolescent mothers and the complications they cause to maternal and neonatal health. This baseline data analysis of the MINA-Brazil birth cohort was conducted in the municipality of Cruzeiro do Sul, state of Acre, Brazil. The chi-square test was used to compare characteristics of adolescent and adult postpartum women, and multiple Poisson regression models with robust variance were used to assess associated factors. Among the postpartum women, 26.2% (95%CI: 24.0-28.4) were adolescents. Factors associated with childbirth in adolescence included: nine years or less of schooling (adjPR:1.36; 95%CI: 1.14-1.61), belongs to the lowest quartiles of the wealth index (1st quartile: adjPR:1.40; 95%CI: 1.08-1.80) (2nd quartile: adjPR:1.37; 95%CI: 1.08-1.74), primigravidae (adjPR:3.69; 95%CI: 2.98-4.57), low pre-pregnancy BMI (adjPR:1.28; CI95%: 1.04-1.57), urinary tract infection during pregnancy (adjPR:1.25; CI95%: 1.07-1.46) and less than six prenatal consultations (adjPR:1.42; 95%CI: 1.21-1.66). Poverty, little schooling, primigravidae, low pre-pregnancy BMI, urinary tract infection during pregnancy and few prenatal consultations were associated with childbirth during adolescence in a municipality in the Northern region of Brazil.

Resumo O objetivo do estudo foi investigar as características socioeconômicas e obstétricas de parturientes adolescentes e suas complicações sobre a saúde materna e neonatal. Trata-se de uma análise de dados da linha de base da coorte de nascimentos MINA-Brasil conduzida no município de Cruzeiro do Sul, estado do Acre. Utilizou-se teste qui-quadrado para comparar características das puérperas adolescentes com as adultas e modelos múltiplos de regressão de Poisson com variância robusta para avaliar fatores associados. Entre as puérperas estudadas, 26,2% (IC95%: 24,0-28,4) eram adolescentes. Os fatores associados ao parto na adolescência foram ter nove anos ou menos de estudo (RPaj:1,36; IC95%: 1,14-1,61), pertencer aos menores quartis do índice de riqueza (1° quartil: RPaj:1,40; IC95%: 1,08-1,80) (2° quartil: RPaj:1,37; IC95%: 1,08-1,74), ser primigesta (RPaj:3,69; IC95%: 2,98-4,57), baixo IMC pré-gestacional (RPaj:1,28; IC95%: 1,04-1,57), infecção urinária na gravidez (RPaj:1,25; IC95%: 1,07-1,46) e menos de seis consultas de pré-natal (RPaj:1,42; IC95%: 1,21-1,66). Pobreza, baixa escolaridade, primigestação, baixo IMC pré-gestacional, infecção urinária na gestação e menor número de consultas de pré-natal foram associados ao parto na adolescência em município da região Norte do Brasil.

Abstract The present study aimed to investigate the socioeconomic and obstetric characteristics of adolescent mothers and the complications they cause to maternal and neonatal health. This baseline data analysis of the MINA-Brazil birth cohort was conducted in the municipality of Cruzeiro do Sul, state of Acre, Brazil. The chi-square test was used to compare characteristics of adolescent and adult postpartum women, and multiple Poisson regression models with robust variance were used to assess associated factors. Among the postpartum women, 26.2% (95%CI: 24.0-28.4) were adolescents. Factors associated with childbirth in adolescence included: nine years or less of schooling (adjPR:1.36; 95%CI: 1.14-1.61), belongs to the lowest quartiles of the wealth index (1st quartile: adjPR:1.40; 95%CI: 1.08-1.80) (2nd quartile: adjPR:1.37; 95%CI: 1.08-1.74), primigravidae (adjPR:3.69; 95%CI: 2.98-4.57), low pre-pregnancy BMI (adjPR:1.28; CI95%: 1.04-1.57), urinary tract infection during pregnancy (adjPR:1.25; CI95%: 1.07-1.46) and less than six prenatal consultations (adjPR:1.42; 95%CI: 1.21-1.66). Poverty, little schooling, primigravidae, low pre-pregnancy BMI, urinary tract infection during pregnancy and few prenatal consultations were associated with childbirth during adolescence in a municipality in the Northern region of Brazil. health MINABrazil MINA Brazil Sul Acre chisquare chi square women factors 262 26 2 26.2 95%CI 95CI CI 95 (95%CI 24.028.4 240284 24.0 28.4 24 0 28 4 24.0-28.4 adolescents included adjPR1.36 adjPR136 adjPR 1.36 1 36 (adjPR:1.36 1.141.61, 114161 1.14 1.61 , 14 61 1.14-1.61) 1st st quartile adjPR1.40 adjPR140 1.40 40 adjPR:1.40 1.081.80 108180 1.08 1.80 08 80 1.08-1.80 2nd nd adjPR1.37 adjPR137 1.37 37 adjPR:1.37 1.081.74, 108174 1.74 74 1.08-1.74) adjPR3.69 adjPR369 3.69 3 69 (adjPR:3.69 2.984.57, 298457 2.98 4.57 98 57 2.98-4.57) prepregnancy pre adjPR1.28 adjPR128 1.28 (adjPR:1.28 CI95% CI95 1.041.57, 104157 1.04 1.57 04 1.04-1.57) adjPR1.25 adjPR125 1.25 25 (adjPR:1.25 1.071.46 107146 1.07 1.46 07 46 1.07-1.46 adjPR1.42 adjPR142 1.42 42 (adjPR:1.42 1.211.66. 121166 1.21 1.66 . 21 66 1.21-1.66) Poverty 26. 9 028 24.028. 24028 240 24. 284 28. 24.0-28. adjPR1 adjPR1.3 adjPR13 136 1.3 (adjPR:1.3 141 1.141.61 11416 114 1.1 161 1.6 6 1.14-1.61 adjPR1.4 adjPR14 140 1.4 adjPR:1.4 081 1.081.8 10818 108 1.0 180 1.8 8 1.08-1.8 137 adjPR:1.3 1.081.74 10817 174 1.7 7 1.08-1.74 adjPR3 adjPR3.6 adjPR36 369 3.6 (adjPR:3.6 984 2.984.57 29845 298 2.9 457 4.5 5 2.98-4.57 adjPR1.2 adjPR12 128 1.2 (adjPR:1.2 CI9 041 1.041.57 10415 104 157 1.5 1.04-1.57 125 071 1.071.4 10714 107 146 1.07-1.4 142 (adjPR:1.4 211 1.211.66 12116 121 166 1.21-1.66 02 24.028 2402 24.0-28 adjPR1. 13 1. (adjPR:1. 1.141.6 1141 11 16 1.14-1.6 adjPR:1. 1.081. 1081 10 18 1.08-1. 1.081.7 17 1.08-1.7 adjPR3. 3. (adjPR:3. 2.984.5 2984 29 2. 45 4. 2.98-4.5 12 1.041.5 1041 15 1.04-1.5 1.071. 1071 1.07-1. 1.211.6 1211 1.21-1.6 24.02 24.0-2 (adjPR:1 1.141. 1.14-1. adjPR:1 1.081 1.08-1 (adjPR:3 2.984. 2.98-4. 1.041. 1.04-1. 1.071 1.07-1 1.211. 1.21-1. 24.0- (adjPR: 1.141 1.14-1 adjPR: 1.08- 2.984 2.98-4 1.041 1.04-1 1.07- 1.211 1.21-1 (adjPR 1.14- 2.98- 1.04- 1.21-

Resumo O objetivo do estudo foi investigar as características socioeconômicas e obstétricas de parturientes adolescentes e suas complicações sobre a saúde materna e neonatal. Trata-se de uma análise de dados da linha de base da coorte de nascimentos MINA-Brasil conduzida no município de Cruzeiro do Sul, estado do Acre. Utilizou-se teste qui-quadrado para comparar características das puérperas adolescentes com as adultas e modelos múltiplos de regressão de Poisson com variância robusta para avaliar fatores associados. Entre as puérperas estudadas, 26,2% (IC95%: 24,0-28,4) eram adolescentes. Os fatores associados ao parto na adolescência foram ter nove anos ou menos de estudo (RPaj:1,36; IC95%: 1,14-1,61), pertencer aos menores quartis do índice de riqueza (1° quartil: RPaj:1,40; IC95%: 1,08-1,80) (2° quartil: RPaj:1,37; IC95%: 1,08-1,74), ser primigesta (RPaj:3,69; IC95%: 2,98-4,57), baixo IMC pré-gestacional (RPaj:1,28; IC95%: 1,04-1,57), infecção urinária na gravidez (RPaj:1,25; IC95%: 1,07-1,46) e menos de seis consultas de pré-natal (RPaj:1,42; IC95%: 1,21-1,66). Pobreza, baixa escolaridade, primigestação, baixo IMC pré-gestacional, infecção urinária na gestação e menor número de consultas de pré-natal foram associados ao parto na adolescência em município da região Norte do Brasil. neonatal Tratase Trata se MINABrasil MINA Brasil Sul Acre Utilizouse Utilizou quiquadrado qui quadrado estudadas 262 26 2 26,2 IC95% IC95 IC (IC95% 24,028,4 240284 24,0 28,4 24 0 28 4 24,0-28,4 RPaj1,36 RPaj136 RPaj 1,36 1 36 (RPaj:1,36 1,141,61, 114161 1,14 1,61 , 14 61 1,14-1,61) 1° (1 quartil RPaj1,40 RPaj140 1,40 40 RPaj:1,40 1,081,80 108180 1,08 1,80 08 80 1,08-1,80 2° (2 RPaj1,37 RPaj137 1,37 37 RPaj:1,37 1,081,74, 108174 1,74 74 1,08-1,74) RPaj3,69 RPaj369 3,69 3 69 (RPaj:3,69 2,984,57, 298457 2,98 4,57 98 57 2,98-4,57) prégestacional pré gestacional RPaj1,28 RPaj128 1,28 (RPaj:1,28 1,041,57, 104157 1,04 1,57 04 1,04-1,57) RPaj1,25 RPaj125 1,25 25 (RPaj:1,25 1,071,46 107146 1,07 1,46 07 46 1,07-1,46 prénatal natal RPaj1,42 RPaj142 1,42 42 (RPaj:1,42 1,211,66. 121166 1,21 1,66 . 21 66 1,21-1,66) Pobreza escolaridade primigestação prégestacional, gestacional, 26, IC9 (IC95 028 24,028, 24028 240 24, 284 28, 24,0-28, RPaj1 RPaj1,3 RPaj13 136 1,3 (RPaj:1,3 141 1,141,61 11416 114 1,1 161 1,6 6 1,14-1,61 ( RPaj1,4 RPaj14 140 1,4 RPaj:1,4 081 1,081,8 10818 108 1,0 180 1,8 8 1,08-1,8 137 RPaj:1,3 1,081,74 10817 174 1,7 7 1,08-1,74 RPaj3 RPaj3,6 RPaj36 369 3,6 (RPaj:3,6 984 2,984,57 29845 298 2,9 457 4,5 9 5 2,98-4,57 RPaj1,2 RPaj12 128 1,2 (RPaj:1,2 041 1,041,57 10415 104 157 1,5 1,04-1,57 125 071 1,071,4 10714 107 146 1,07-1,4 142 (RPaj:1,4 211 1,211,66 12116 121 166 1,21-1,66 (IC9 02 24,028 2402 24,0-28 RPaj1, 13 1, (RPaj:1, 1,141,6 1141 11 16 1,14-1,6 RPaj:1, 1,081, 1081 10 18 1,08-1, 1,081,7 17 1,08-1,7 RPaj3, 3, (RPaj:3, 2,984,5 2984 29 2, 45 4, 2,98-4,5 12 1,041,5 1041 15 1,04-1,5 1,071, 1071 1,07-1, 1,211,6 1211 1,21-1,6 (IC 24,02 24,0-2 (RPaj:1 1,141, 1,14-1, RPaj:1 1,081 1,08-1 (RPaj:3 2,984, 2,98-4, 1,041, 1,04-1, 1,071 1,07-1 1,211, 1,21-1, 24,0- (RPaj: 1,141 1,14-1 RPaj: 1,08- 2,984 2,98-4 1,041 1,04-1 1,07- 1,211 1,21-1 (RPaj 1,14- 2,98- 1,04- 1,21-

ABSTRACT Using a panel study design, we aimed to estimate the seroconversion and seroreversion rates of anti-Strongyloides IgG antibodies from surveys carried out 11 months apart in a rural community in the Amazon Basin in Brazil. We used enzyme immunoassays to measure anti-Strongyloides IgG antibodies in 325 baseline plasma samples and 224 others that were collected 11 months later from residents in the agricultural settlement of Granada, Acre State. We observed anti-Strongyloides IgG antibodies in 21.8% of the baseline samples (which showed that 3.4% of participants had larvae in their stool) and in 23.7% of the follow-up samples. The seroconversion rate estimated at 9.7 episodes/100 person-years at risk agrees with ongoing transmission. Specific antibodies were relatively short-lived and nine (25.0%) of 36 seropositive participants at baseline were seronegative when retested 11 months later. Fecal surveys can severely underestimate the prevalence of S. stercoralis infection in rural Amazonians. Serology provides a field-deployable diagnostic tool to find high-prevalence populations, identify associated risk factors, and monitor intervention programs. design antiStrongyloides anti Strongyloides 1 Brazil 32 22 Granada State 218 21 8 21.8 which 34 3 4 3.4 stool 237 23 7 23.7 followup follow up 97 9 9. episodes100 episodes 100 episodes/10 personyears person years transmission shortlived short lived 25.0% 250 25 0 (25.0% S Amazonians fielddeployable field deployable highprevalence high populations factors programs 2 21. 3. 23. episodes10 10 episodes/1 25.0 (25.0 episodes1 episodes/ 25. (25. (25 (2 (

BACKGROUND Malaria remains common among native Amazonians, challenging Brazil′s elimination efforts. OBJECTIVES We examined the epidemiology of malaria in riverine populations of the country′s main hotspot - the upper Juruá Valley in Acre state, close to the Brazil-Peru border, where Plasmodium vivax accounts for > 80% of cases. METHODS Participants (n = 262) from 10 villages along the Azul River were screened for malaria parasites by microscopy and genus-specific, cytochrome b (cytb) gene-based polymerase chain reaction. Positive samples were further tested with quantitative TaqMan assays targeting P. vivax- and P. falciparum-specific cytb domains. We used multiple logistic regression analysis to identify independent correlates of P. vivax infection. FINDINGS Microscopy detected only one P. vivax and two P. falciparum infections. TaqMan assays detected 33 P. vivax infections (prevalence, 11.1%), 78.1% of which asymptomatic, with a median parasitaemia of 34/mL. Increasing age, male sex and use of insecticide-treated bed nets were significant predictors of elevated P. vivax malaria risk. Children and adults were similarly likely to remain asymptomatic once infected. MAIN CONCLUSIONS Our findings are at odds with the hypothesis of age-related clinical immunity in native Amazonians. The low virulence of local parasites is suggested as an alternative explanation for subclinical infections in isolated populations.

Abstract INTRODUCTION: The Amazon tropical rainforest has the most dense and diverse ecosystem worldwide. A few studies have addressed rodent-borne diseases as potential hazards to humans in this region. METHODS: A retrospective survey was conducted using enzyme-linked immunosorbent assay for detecting mammarenavirus and orthohantavirus antibodies in 206 samples collected from rural settlers of the Brazilian Western Amazonian region. RESULTS: Six (2.91%) individuals in the age group of 16 to 36 years were found to possess antibodies against mammarenavirus. CONCLUSION: Evidence of previous exposure to mammarenavirus in the rural population points to its silent circulation in this region.

The global emergence of Plasmodium vivax strains resistant to chloroquine (CQ) since the late 1980s is complicating the current international efforts for malaria control and elimination. Furthermore, CQ-resistant vivax malaria has already reached an alarming prevalence in Indonesia, East Timor and Papua New Guinea. More recently, in vivo studies have documented CQ-resistant P. vivax infections in Guyana, Peru and Brazil. Here, we summarise the available data on CQ resistance across P. vivax-endemic areas of Latin America by combining published in vivo and in vitro studies. We also review the current knowledge regarding the molecular mechanisms of CQ resistance in P. vivax and the prospects for developing and standardising reliable molecular markers of drug resistance. Finally, we discuss how the Worldwide Antimalarial Resistance Network, an international collaborative effort involving malaria experts from all continents, might contribute to the current regional efforts to map CQ-resistant vivax malaria in South America.

Enhanced understanding of the transmission dynamics and population genetics for Plasmodium vivax is crucial in predicting the emergence and spread of novel parasite phenotypes with major public health implications, such as new relapsing patterns, drug resistance and increased virulence. Suitable molecular markers are required for these population genetic studies. Here, we focus on two groups of molecular markers that are commonly used to analyse natural populations of P. vivax. We use markers under selective pressure, for instance, antigen-coding polymorphic genes, and markers that are not under strong natural selection, such as most minisatellite and microsatellite loci. First, we review data obtained using genes encoding for P. vivax antigens: circumsporozoite protein, merozoite surface proteins 1 and 3α, apical membrane antigen 1 and Duffy binding antigen. We next address neutral or nearly neutral molecular markers, especially microsatellite loci, providing a complete list of markers that have already been used in P. vivax populations studies. We also analyse the microsatellite loci identified in the P. vivax genome project. Finally, we discuss some practical uses for P. vivax genotyping, for example, detecting multiple-clone infections and tracking the geographic origin of isolates.

To infer recent patterns of malaria transmission, we measured naturally acquired IgG antibodies to the conserved 19-kDa C-terminal region of the merozoite surface protein (MSP)-1 of both Plasmodium vivax (PvMSP-1(19)) and Plasmodium falciparum (PfMSP-1(19)) in remote malaria-exposed populations of the Amazon Basin. Community-based cross-sectional surveys were carried out between 2002 and 2003 in subjects of all age groups living along the margins of the Unini and Jaú rivers, Northwestern Brazil. We found high prevalence rates of IgG antibodies to PvMSP-1(19) (64.0 - 69.6%) and PfMSP-1(19) (51.6 - 52.0%), with significant differences in the proportion of subjects with antibodies to PvMSP-1(19) according to age, place of residence and habitual involvement in high-risk activities, defining some groups of highly exposed people who might be preferential targets of malaria control measures. In contrast, no risk factor other than age was significantly associated with seropositivity to PfMSP-1(19). Only 14.1% and 19.3% of the subjects tested for antibodies to PvMSP-1(19) and PfMSP-1(19) in consecutive surveys (142 - 203 days apart) seroconverted or had a three fold or higher increase in the levels of antibodies to these antigens. We discuss the extent to which serological data correlated with the classical malariometric indices and morbidity indicators measured in the studied population at the time of the seroprevalence surveys and highlight some limitations of serological data for epidemiological inference.

As prevalências de desnutrição, parasitoses intestinais, anemia e deficiência de ferro foram avaliadas, por meio de inquéritos transversais de base populacional, em pré-escolares na área urbana de dois municípios das Amazônia Ocidental Brasileira, Assis Brasil (n = 200) e Acrelândia (n = 477). Os índices antropométricos peso/estatura (P/E), peso/idade (P/I) e estatura/idade (E/I) foram calculados como escores z, com base na população de referência do National Center for Health Statistics de 1977. Diagnosticaram-se déficits nutricionais segundo índices P/E, P/I e E/I em 3,7%, 8,7% e 7,5% das crianças, respectivamente. Encontraram-se parasitas intestinais em 32,5% das 554 amostras examinadas, sendo raras as infecções por helmintos. Com base nos níveis de hemoglobina sangüínea e níveis plasmáticos de ferritina e receptor solúvel de transferrina, diagnosticaram-se anemia e deficiência de ferro em 30,6% e 43,5% das crianças, respectivamente. Somente 47,6% das crianças com deficiência de ferro tinham anemia, indicando que a medida isolada de hemoglobina sangüínea subestima a magnitude da deficiência de ferro nesta população. Nos dois municípios, a anemia e a desnutrição foram significantemente mais prevalentes entre as crianças de famílias dos estratos sócio-econômicos mais baixos.

The article presents prevalence rates for malnutrition, intestinal parasitic infections, anemia, and iron deficiency in under-five children in a population-based cross-sectional survey performed in the urban area of two counties in the Western Brazilian Amazon, Assis Brasil (n = 200) and Acrelandia (n = 477). Available data included: (a) weight and height measurements, standardized as z-scores using the 1977 NCHS reference population, (b) diagnosis of current intestinal parasitic infection, (c) blood hemoglobin levels, and (d) plasma ferritin and soluble transferrin receptor levels. Overall prevalence rates of low weight-for-height, low weight-for-age, and low height-for-age were 3.7%, 8.7%, and 7.5%, respectively, with similar figures in the two towns. Intestinal parasites were detected in 32.5% children; helminths were uncommon. Anemia and iron deficiency were diagnosed in 30.6% and 43.5% of the children, respectively. Evidence of anemia was found in only 47.6% of the children with depleted iron reserves, indicating that hemoglobin measurement alone would severely underestimate the magnitude of iron deficiency in this population. In both towns, anemia and malnutrition were significantly more prevalent among children in the lowest socioeconomic stratum.

As prevalências de desnutrição, parasitoses intestinais, anemia e deficiência de ferro foram avaliadas, por meio de inquéritos transversais de base populacional, em pré-escolares na área urbana de dois municípios das Amazônia Ocidental Brasileira, Assis Brasil (n = 200) e Acrelândia (n = 477). Os índices antropométricos peso/estatura (P/E), peso/idade (P/I) e estatura/idade (E/I) foram calculados como escores z, com base na população de referência do National Center for Health Statistics de 1977. Diagnosticaram-se déficits nutricionais segundo índices P/E, P/I e E/I em 3,7%, 8,7% e 7,5% das crianças, respectivamente. Encontraram-se parasitas intestinais em 32,5% das 554 amostras examinadas, sendo raras as infecções por helmintos. Com base nos níveis de hemoglobina sangüínea e níveis plasmáticos de ferritina e receptor solúvel de transferrina, diagnosticaram-se anemia e deficiência de ferro em 30,6% e 43,5% das crianças, respectivamente. Somente 47,6% das crianças com deficiência de ferro tinham anemia, indicando que a medida isolada de hemoglobina sangüínea subestima a magnitude da deficiência de ferro nesta população. Nos dois municípios, a anemia e a desnutrição foram significantemente mais prevalentes entre as crianças de famílias dos estratos sócio-econômicos mais baixos.

The article presents prevalence rates for malnutrition, intestinal parasitic infections, anemia, and iron deficiency in under-five children in a population-based cross-sectional survey performed in the urban area of two counties in the Western Brazilian Amazon, Assis Brasil (n = 200) and Acrelandia (n = 477). Available data included: (a) weight and height measurements, standardized as z-scores using the 1977 NCHS reference population, (b) diagnosis of current intestinal parasitic infection, (c) blood hemoglobin levels, and (d) plasma ferritin and soluble transferrin receptor levels. Overall prevalence rates of low weight-for-height, low weight-for-age, and low height-for-age were 3.7%, 8.7%, and 7.5%, respectively, with similar figures in the two towns. Intestinal parasites were detected in 32.5% children; helminths were uncommon. Anemia and iron deficiency were diagnosed in 30.6% and 43.5% of the children, respectively. Evidence of anemia was found in only 47.6% of the children with depleted iron reserves, indicating that hemoglobin measurement alone would severely underestimate the magnitude of iron deficiency in this population. In both towns, anemia and malnutrition were significantly more prevalent among children in the lowest socioeconomic stratum.

Estudo de base populacional sobre a prevalência e distribuição de parasitoses intestinais foi realizado em assentamento agrícola na Amazônia Brasileira (Granada, Acre). Mais da metade (53,4%) das 429 amostras analisadas de indivíduos de todas as idades, moradores de 113 domicílios, continha cistos, ovos ou larvas de parasitas intestinais. Os parasitas intestinais de maior prevalência foram Giardia duodenalis (19,6%) e os geo-helmintos (12,7%); 105 (24,5%) indivíduos apresentavam co-infecção por mais de uma espécie de parasita. Houve diferença significativa em relação à idade na prevalência somente de G. duodenalis (crianças < 1 ano e adultos > 30 anos foram menos afetados). Seis domicílios (5,3%), localizados num raio de 690m, concentraram 48,1% de todos os indivíduos infectados por geo-helmintos na área de estudo. Domicílios incluídos nesse agregado (cluster) eram mais pobres e com maior número de habitantes do que os domicílios localizados fora do agregado. A distribuição espacial dos agregados de casos de infecção por geo-helmintos fornece informações valiosas para intervenções sanitárias na comunidade da área de estudo.

A population-based survey of the prevalence and spatial distribution of intestinal parasitism was carried out in an agricultural settlement in the Amazon Basin of Brazil (Granada, Acre State). More than half (53.4%) of the 429 stool specimens from subjects in all age groups, living in 113 households, had cysts, ova, or larvae of intestinal parasites. The most prevalent parasites were Giardia duodenalis (19.6%) and soil-transmitted helminths (12.7%); 105 (24.5%) subjects were infected with more than one species of parasite. Significant age-related differences in prevalence were only found for G. duodenalis (children < 1 year and adults > 30 years were less affected). Six households (5.3%), situated within a radius of 690m, comprised 48.1% of all subjects harboring soil-transmitted helminths in our study area. Households within this cluster were poorer and more crowded than those outside the cluster. The observed spatial clustering of infections with soil-transmitted helminths provides valuable information for the spatial targeting of sanitary interventions in this area.

Estudo de base populacional sobre a prevalência e distribuição de parasitoses intestinais foi realizado em assentamento agrícola na Amazônia Brasileira (Granada, Acre). Mais da metade (53,4%) das 429 amostras analisadas de indivíduos de todas as idades, moradores de 113 domicílios, continha cistos, ovos ou larvas de parasitas intestinais. Os parasitas intestinais de maior prevalência foram Giardia duodenalis (19,6%) e os geo-helmintos (12,7%); 105 (24,5%) indivíduos apresentavam co-infecção por mais de uma espécie de parasita. Houve diferença significativa em relação à idade na prevalência somente de G. duodenalis (crianças < 1 ano e adultos > 30 anos foram menos afetados). Seis domicílios (5,3%), localizados num raio de 690m, concentraram 48,1% de todos os indivíduos infectados por geo-helmintos na área de estudo. Domicílios incluídos nesse agregado (cluster) eram mais pobres e com maior número de habitantes do que os domicílios localizados fora do agregado. A distribuição espacial dos agregados de casos de infecção por geo-helmintos fornece informações valiosas para intervenções sanitárias na comunidade da área de estudo.

A population-based survey of the prevalence and spatial distribution of intestinal parasitism was carried out in an agricultural settlement in the Amazon Basin of Brazil (Granada, Acre State). More than half (53.4%) of the 429 stool specimens from subjects in all age groups, living in 113 households, had cysts, ova, or larvae of intestinal parasites. The most prevalent parasites were Giardia duodenalis (19.6%) and soil-transmitted helminths (12.7%); 105 (24.5%) subjects were infected with more than one species of parasite. Significant age-related differences in prevalence were only found for G. duodenalis (children < 1 year and adults > 30 years were less affected). Six households (5.3%), situated within a radius of 690m, comprised 48.1% of all subjects harboring soil-transmitted helminths in our study area. Households within this cluster were poorer and more crowded than those outside the cluster. The observed spatial clustering of infections with soil-transmitted helminths provides valuable information for the spatial targeting of sanitary interventions in this area.

Descrevem-se aqui dados de prevalência de malária e soroprevalência de arboviroses na linha de base de um estudo de coorte, envolvendo 467 indivíduos, atualmente em curso na Amazônia rural. A maioria (72,2%) dos indivíduos relatou um ou mais episódios maláricos prévios, tendo 15,6% deles tido pelo menos uma internação hospitalar motivada pela malária, mas somente 3,6% dos indivíduos com idade igual ou superior a cinco anos tinham plasmódios (Plasmodium vivax em dez e P. falciparum em quatro) diagnosticados por microscopia. Detectaram-se anticorpos contra Alphavirus, Orthobunyavirus e Flavivirus, pelo teste de inibição de hemaglutinação, em 42,6% dos indivíduos com idade igual ou superior a cinco anos, com uma taxa de soropositividade maior entre indivíduos do sexo masculino (49,2%) do que os do feminino (36,2%). Como 98,9% dos indivíduos entrevistados tinham sido previamente vacinados contra a febre amarela, a presença de reações cruzadas com o vírus do dengue e outros Flavivirus não pode ser descartada, mas a exposição recente ao vírus do dengue foi evidenciada pela detecção de anticorpos IgM por ELISA em 12 indivíduos (3,3%).

The authors describe the baseline malaria prevalence and arbovirus seroprevalence among 467 subjects in an ongoing cohort study in rural Amazonia. Most subjects (72.2%) reported one or more previous episodes of malaria, and 15.6% had been hospitalized for malaria, but only 3.6% of individuals five years or older had malaria parasites detected by microscopy (10 with Plasmodium vivax and 4 with P. falciparum). Antibodies to Alphavirus, Orthobunyavirus, and/or Flavivirus were detected by hemagglutination inhibition (HI) in 42.6% of subjects aged five years or older, with a higher seropositivity rate among males (49.2%) than females (36.2%). Since 98.9% of subjects had been immunized for yellow fever, the presence of cross-reactive antibodies to dengue and other Flaviviruses cannot be ruled out, but at least 12 subjects (3.3%) with IgM antibodies to dengue virus detected by ELISA had a putative recent exposure to this virus.