Resumen Este estudio tuvo como objetivo evaluar el efecto de la inoculación con L. buchneri liofilizado y/o activado sobre las poblaciones de bacterias de ácido láctico en ensilajes de maíz. Los tratamientos experimentales consistieron en ensilaje de maíz sin aditivo y ensilaje con los inoculantes L. buchneri 1 × 10 5 ufc/g, aplicados según las recomendaciones del fabricante (1 g/tonelada de forraje) en las formas de inoculante liofilizado e inoculante preactivado. Se identificaron aislados purificados de ensilajes de maíz con y sin inocu lación, y el 93% de los aislados correspondieron a bacterias de ácido láctico de la especie L. plantarum. Entre los aislados no se detectaron bacterias de la especie L. buchneri. La aplicación de L. buchneri liofilizado o activado en la masa ensilada promueve un aumento considerable de la población de bacterias de ácido lácticas en los ensilajes de maíz durante todo el período de fermentación.

Abstract This study aimed to evaluate the effect of inoculation with lyophilised and/or activated Lactobacillus buchneri on lactic acid bacteria populations in corn silage. Experimental treatments consisted of corn silage without additives or silage with the inoculants of L. buchneri (1 x 10 5 cfu/g) applied according to the manufacturer’s recommendations (1 g/tonne fodder) in the forms of the lyophilised inoculant and pre-activated inoculant. Purified isolates from corn silage with and without the inoculant were identified, and 93% of the isolates corresponded to the lactic acid bacteria of the species Lactobacillus plantarum. Among the isolates, no bacteria of the species L. buchneri were detected. The application of lyophilised or activated L. buchneri improved the microbiological profile and reduced ethanol production in corn silage, even without being identified among the isolates captured 70 days after ensilage.

The objective of this study was to evaluate the morphogenetic and structural characteristics of Piatã grass under rotational stocking and nitrogen fertilization during the seasons. A randomized complete block design in a split-plot arrangement with three replications was used. The main plots were the applications of 0, 150, 300 and 450 kg per ha of N in the form of urea, and the subplots were seasons of the year: late summer/fall, winter, spring and summer. No interaction was detected between nitrogen fertilization and season for leaf appearance rate, leaf lifespan, number of live leaves and final length leaves. However, an interaction (P<0.05) of nitrogen fertilization and season influenced leaf elongation rate, phyllochron, leaf senescence rate and stem elongation rate. The leaf elongation rate and leaf appearance rate were linearly affected (P<0.05) by nitrogen fertilization. The seasons affected (P<0.05) the leaf lifespan and number of life leaves. The leaf lifespan decreased by 0.06 days for each kg of N applied. On the other hand, the number of live leaves increased by 0.0026 leaves/tiller for each kg of N. Fertilization with nitrogen positively affects morphogenetic and structural characteristics of Piatã grass under rotational stocking. This effect can be optimized during rainy periods in spring and late summer/autumn.

El objetivo de este estudio fue evaluar las características morfogenéticas y estructurales del pasto Piatã bajo pastoreo rotacional y fertilización nitrogenada durante las diferentes estaciones del año. Se utilizó un diseño de bloques completos al azar con un arreglo de parcelas divididas con tres repeticiones. Las parcelas principales fueron las aplicaciones de 0, 150, 300 y 450 kg/ha de N en forma de urea, y las subparcelas fueron las estaciones del año: finales de verano/otoño, invierno, primavera y verano. No se detectó interacción entre la fertilización nitrogenada y la estación para la tasa de aparición de hojas, la esperanza de vida de la hoja, el número de hojas vivas y longitud final de las hojas. Sin embargo, una interacción (P<0,05) de la fertilización nitrogenada y la temporada influyeron en la tasa de elongación de la hoja, filocrono, tasa de senescencia foliar y tasa de elongación del tallo. La tasa de elongación de la hoja y la tasa de aparición de la hoja se vieron afectadas linealmente (P<0,05) por la fertilización nitrogenada. Se observó un efecto (P<0,05) de las estaciones sobre la vida media foliar y el número de hojas vivas. La vida útil de la hoja disminuyó en 0,06 días por cada kg de N aplicado. Por otro lado, el número de hojas vivas aumentó en 0,0026 hoja/macollo por cada kg de N. La fertilización con nitrógeno influye positivamente en las características morfogenéticas y estructurales del pasto Piatã bajo pastoreo rotativo. Este efecto se puede optimizar durante los períodos de lluvia, en la primavera y al final del verano/otoño.

Abstract This study aimed to measure the carbohydrate and protein fractioning of silage of Brachiaria brizantha cv. Marandu (BBS). The experiment was a completely randomized design with five treatments and five repetitions to evaluate the inclusion of dehydrated brewery residue (DBR) to Marandu grass silage at levels of 0, 10, 20, 30 and 40% fresh matter basis. The DBR inclusion resulted in a linear reduction (P<0.05) of total carbohydrate content, which decreased 0.35% per unit of residue added. The content of fraction B2 decreased linearly as DBR was added (P<0.05). Nitrogen content increased linearly when residue was added to silage of Marandu grass (P<005). Fraction A, obtained from Marandu grass, presented a linear increasing behavior according to the inclusion of DBR. This inclusion promoted a linear decrease (P<0.05) of fraction B1+B2, which resulted in a 0.37% reduction for each 1% of byproduct added. Dehydrated brewery residue is a good source of additives for ensiling Marandu grass, by considerably improving fractions of proteins and carbohydrates. The level of addition of 40% is recommended for providing better nutritional value to silage.

Resumen Este estudio tuvo como objetivo medir el fraccionamiento de carbohidratos y proteínas del ensilaje de Brachiaria brizantha cv. Marandu (BBS). El experimento fue un diseño completamente al azar con cinco tratamientos y cinco repeticiones para evaluar la inclusión de residuos de cervezas deshidratadas (DBR) en ensilaje de hierba Marandu en niveles de 0, 10, 20, 30 y 40% de materia fresca. La inclusión de DBR resultó en una reducción lineal (P <0.05) del contenido total de carbohidratos, que disminuyó 0.35% por unidad de residuo agregado. El contenido de la fracción B2 disminuyó linealmente a medida que se agregaba DBR (P<0.05). El contenido de nitrógeno aumentó linealmente cuando se añadió residuo al ensilaje del pasto Marandu (P <005). La fracción A, obtenida del pasto Marandu, presentó un comportamiento de aumento lineal de acuerdo con la inclusión de DBR. Esta inclusión promovió una disminución lineal (P<0.05) de la fracción B1 + B2, que resultó en una reducción de 0.37% por cada 1% de subproducto agregado. Los residuos de cervecería deshidratada son una buena fuente de aditivos para ensilar el pasto Marandu, mejorando considerablemente las fracciones de proteínas y carbohidratos. Se recomienda un nivel de adición del 40% para proporcionar un mejor valor nutritivo al ensilaje.

RESUMO Objetivou-se avaliar os efeitos do farelo de arroz sobre as perdas por gases e efluente, o perfil fermentativo e a composição química das silagens de Brachiaria decumbens cv. Basilisk ensilado em silos de laboratório. Utilizou-se delineamento inteiramente casualizado, com cinco tratamentos: 0; 10; 20, 30 e 40g/kg de farelo de arroz, com base na matéria natural da gramínea. Os valores das perdas por gases e efluentes foram reduzidos linearmente em 0,11 e 0,39g/kg MS, enquanto a recuperação da matéria seca aumentou linearmente em 0,32g/kg MS. Houve comportamento quadrático nos valores de pH e, redução linear para os valores de N-amoniacal e poder tampão, estimados em 1,1 e 0,5g/kg MS. As concentrações de ácido acético, butírico e propiônico foram reduzidas linearmente em 0,1; 0,008 e 0,006g/kg MS, enquanto a de ácido láctico aumentou linearmente em 0,5g/kg MS. Cada unidade porcentual de farelo de arroz promoveu aumento linear de 4,3; 1,8; 7,1 e 7,0g/kg nos teores de matéria seca, proteína bruta, extrato etéreo e nutrientes digestíveis totais, e redução linear de 6,9; 4,3 e 2,6g/kg MS nos teores de fibra em detergente neutro, fibra em detergente ácido e hemicelulose. A inclusão de 10g/kg de farelo de arroz já assegura diminuição das perdas por gases e efluentes, aumento na recuperação de matéria seca, proporcionando melhorias no perfil fermentativo e na composição química das silagens.

SUMMARY The objective was to assess the effects of rice meal over losses by gas and effluent, the fermentative profile and the chemical composition of Brachiaria decumbens cv. Basilisk silage in laboratory bags. Entirely random lining was used, with five treatments: 0; 10; 20; 30 and 40g/kg rice meal, based on natural grass matter. The values of losses by gas and effluents were reduced linearly in 0.11 and 0.39g/kg DM, while recovery of dry matter increased linearly in 0.32g/kg DM. There was quadratic behavior for pH values and linear decrease for the values of N-ammoniacal and buffering power estimated in 1.1 and 5g/kg DM. Concentrations of acetic, butyric and propionic acids were reduced linearly in 0.1; 0.008 and 0.006g/kg DM, while lactic acid increased linearly in 0.5g/kg DM. Each 1g/kg rice meal promoted linear increase of 4.3; 1.8; 7.1 and 7.0g/kg dry matter levels, crude protein, ethereal extract and total digestible nutrients and linear decrease of 6.9; 4.3 and 2.6g/kg DM of fiber in neutral detergent, fiber in acidic detergent and hemicellulose levels. Inclusion of 10g/kg rice meal already ensures lowering losses by gas and effluents, increase in dry matter recovery, allowing improvements on the fermentative profile and the chemical composition of silage.

Resumo: O objetivo deste trabalho foi avaliar o efeito da alimentação suplementar de resíduos de feijão no desempenho produtivo e nos parâmetros sanguíneos de vacas leiteiras em pastejo. Foram utilizadas oito vacas mestiças, com peso médio de 500 kg, distribuídas em duplo quadrado latino 4×4, alimentadas com alimentação suplementar de 134, 240, 348 e 449 g kg-1 de resíduos de feijão na ração concentrada. Os animais receberam a suplementação após a ordenha no período da manhã e da tarde. A inclusão de resíduos de feijão não alterou os pesos corporais médios e os escores corporais; no entanto, reduziu a produção de leite. Os teores de gordura, proteína bruta, lactose e extrato seco total não foram alterados pela inclusão dos resíduos de feijão. A concentração sanguínea de glicose e colesterol não foi alterada pela adição do resíduo antes do fornecimento de ração concentrada e nem quatro e seis horas após essa alimentação. A inclusão dos resíduos de feijão na ração concentrada de vacas leiteiras em pastejo, com produção média diária de 13 kg, reduz a produção de leite. Todavia, a utilização desses resíduos pouco influencia a composição do leite e os parâmetros sanguíneos.

Abstract: The objective of this work was to evaluate the effect of dietary supplement bean residue on growth performance and blood parameters of dairy cows grazing. Eight crossbred cows weighing 500 kg were analyzed, distributed in 4×4 double Latin square, and fed with 134, 240, 348, and 449 g kg-1 of a supplementary diet of bean waste in the concentrated feed. The animals received the supplementation after milking in the morning and in the afternoon. The inclusion of bean residues did not change average body weights and body scores; however, it reduced the milk production. Fat, protein, lactose, and total solid contents (g kg-1) were not changed by bean waste inclusion. Blood glycose and cholesterol did not change before the offering of the concentrated feed diet, nor after four to six hours of feeding this diet. The inclusion of bean residues in the concentrated feed of dairy cows subjected to grazing, with a daily average production of 13 kg, reduces milk production. However, the use of these residues has little effect on milk composition and blood parameters.

The objective of this experiment was to evaluate the losses of nutrient through gases and effluents, the nutrient recovery, fermentation parameters and the chemical parameters of silages of Brachiaria decumbens with the addition of soybean hulls, testing five levels of inclusion: 0, 10, 20, 30 and 40% (as is), with five replications per treatment. After the standardization cut of the forage, performed at 5 cm from the soil level, the soil was fertilized with nitrogen and potassium in the form of ammonium sulfate and potassium chloride, respectively, at 60 kg/ha. After 60 days, the grass was cut at 10 cm from the soil and ensiled. Experimental silos with capacity of 10 liters with Bunsen valve were used. Silos had 3 kg of sand conditioned at the bottom for retention of the effluents. The results show that the contents of dry matter, crude protein, acid detergent insoluble fiber and total digestible nutrients presented positive linear behavior with the inclusion of soybean hulls. In the grass silages with addition of soybean hulls, there was greater recovery of dry matter in relation to the control silage, which is a reflection of the negative linear behavior for the values of losses of gases and effluent. There was quadratic behavior in the pH values and negative linear behavior for the ammonia N values and buffering capacity with the inclusion of the additive in the grass silage. Inclusion of soybean hulls is sufficient to improve the fermentation pattern and minimize the losses through gases and effluents, contributing to the nutrient recovery, in addition to promoting overall improvement of the nutritional value of Brachiaria grass silages.

This experiment was conducted to evaluate the protein and carbohydrate fractions, and to estimate the in situ rumen degradation kinetic parameters of Brachiaria decumbens grass silage with inclusion of rice bran. Five rice bran inclusion levels were tested: 0, 10, 20, 30 and 40% of the natural matter, distributed into a completely randomized design with five replications. The brachiaria grass was obtained at 60 days of regrowth by cutting the forage at 10 cm from the soil and ensiling it in 10-L experimental silo bags, which were opened after 40 days. The degradability profiles for DM, CP, NDF and of the feeds for each animal utilized made it possible to obtain the estimates of the parameters analyzed. The contents of total carbohydrates (TCH), fibrous carbohydrates and fraction A+B1 of the TCH decreased linearly as the rice bran levels were elevated. Fraction A of the protein was increased linearly by 0.64% for every 1% of rice bran added to the silage. For nitrogenous fractions B3 and C, there was a decrease of 0.11 and 0.40% for each 1% of rice bran added to the silage, respectively. For the rumen degradability parameters of the dry matter, fractions A and I had estimated increases of 0.54 and 0.04% for every 1% inclusion of rice bran. For the degradation rate of fraction B (c), treatments had no effect. Fractions A and I of the crude protein degradability parameters increased by 0.22 and 0.72%, respectively, with inclusion of 1% rice bran. Inclusion levels of rice bran had no effect on fraction D, on the degradation rate of fraction B (c) or lag time (L) estimated for neutral detergent fiber. Inclusion of 10 and 20% rice bran in the brachiaria grass silage improves protein and carbohydrate fractions and rumen degradation kinetic parameters of dry matter, crude protein and neutral detergent fiber.

Foi conduzido um experimento para avaliar o comportamento ingestivo de eqüinos sob pastejo em pastagens de Paspalum notatum e Brachiaria decumbens. O delineamento experimental foi o inteiramente casualizado, com dois pastos representando os tratamentos, cada um com cinco repetições. O período experimental foi de 40 dias, sendo 30 para adaptação dos animais e 10 para avaliações, que consistiram de três, com duração de 24 horas cada, em intervalos de cinco dias. Os cavalos passaram mais tempo pastejando no período diurno no pasto de Brachiaria decumbens (10,58 horas) em relação ao tempo passado no pasto de Paspalum notatum (7,69 horas). Com relação ao tempo de pastejo noturno, a situação se inverteu: os animais pastejaram maior tempo durante o dia e diminuíram o ritmo durante a noite (2,91 horas de pastejo) no pasto de Brachiaria decumbens. O tempo de ócio noturno foi superior no Paspalum notatum (4,15 horas). Os animais apresentaram maior número de bocados (22.720) no pastejo do capim Paspalum notatum comparado ao Brachiaria decumbens, no qual diminuiu-se a quantidade de bocados (17166).

It was carried out an experiment to evaluate the ingestive behavior of horses grazing Paspalum notatum and Brachiaria decumbens pastures. It had been used a completely randomized experimental design, with two treatments (two pastures) and five replicates per treatment. The experiment lasted 40 days, consisting of 30 days for adaptation of animals on the pastures and 10 days to evaluate grazing behaviour, with three periods of 24 hours and intervals of five days. The horses spent more time grazing during the day on the Brachiaria decumbens pasture (10.58 hours) than did on the Paspalum notatum pasture (7.69 hours). At night, the situation was reversed, horses grazed less (2.91 hours) on the Brachiaria decumbens pasture. Overnight leisure time was larger on the Paspalum notatum pasture (4.15 hours) than Brachiaria decumbens pasture (1.42 hours). The higher total daily bites had been observed (22720) in the animals on Paspalum notatum when compared with Brachiaria decumbens where they decreased the quantity of bites (17166).

Foi conduzido um experimento com o objetivo de avaliar o comportamento de bezerros em pastagens de Brachiaria brizantha e Brachiaria decumbens, no sistema de lotação contínua com taxa de lotação variável. O delineamento experimental foi o inteiramente casualizado, com dois pastos representando os tratamentos, cada um com cinco repetições. O período experimental foi de 30 dias, sendo 20 para adaptação dos animais e 10 para avaliações, que consistiram de três, com duração de 24h cada, em intervalos de cinco dias. Os bezerros pastejaram menos tempo no pasto de Brachiaria brizantha, com tempo de 9,75h, enquanto que o pastejo na Brachiaria decumbens foi de 11,3h. Comportamento diferente foi observado para o tempo de ruminação: 6,8 e 6,4 para os pastos de Brachiaria brizantha e Brachiaria decumbens, respectivamente. O número de bocados por minuto foi menor para a Brachiaria decumbens, 31,15, enquanto que, para a Brachiaria brizantha, os bezerros pastejaram com uma taxa de bocados de 34,91 bocados por minuto. Os bezerros tiveram um ganho de peso médio diário superior no pasto de Brachiaria brizantha (390g dia-1), enquanto que, para a Brachiaria decumbens, o ganho diário foi de 340g dia-1.

An experiment was carried out to evaluate the ingestive behavior of calves on Brachiaria brizantha and Brachiaria decumbens pastures, under continuous stocking with variable stocking rate. The experimental design was entirely randomized, with two treatments (two pastures), and five replicates per treatment. The experimental period lasted 30 days, consisting of days 20 for adaptation of animals and 10 for behavioral evaluations that consisted of three periods of 24:00h each, in intervals of five days. The calves spent less time grazing Brachiaria brizantha pasture (9,8h) than they did on the Brachiaria decumbens (11,3h). Different rumination times had been observed, 6,8 and 6,4 h for the animals grazing Brachiaria brizantha and Brachiaria decumbens, respectively. We suggested that the selective behavior of the calves on the Brachiaria decumbens made them to eat good quality grass, thus they spent a similar rumination time to that observed for Brachiaria brizantha. The number of bites per minute was inferior for the Brachiaria decumbens, 31,2h, when compared with Brachiaria brizantha (the mean bite rate was 34,9 bites per minute). The calves had a larger daily gain to Brachiaria brizantha (390g day-1) than they had to Brachiaria decumbens (340g day-1).