Resumo Objetivos: analisar a tendência temporal e a distribuição espacial da mortalidade infantil em Pernambuco no período de 2001 a 2019. Métodos: estudo ecológico envolvendo todos os óbitos em crianças menores de um ano residentes no estado. Os dados foram coletados no Sistema de Informações sobre Mortalidade e no Sistema de Informação sobre Nascidos Vivos. Foram calculados quatro coeficientes de mortalidade e a análise temporal foi realizada aplicando o modelo de regressão por pontos de inflexão dos coeficientes em diferentes níveis espaciais. Resultados: foram registrados 47.949 óbitos, dos quais 51,0% (n=24.447) ocorreram nos seis primeiros dias de vida. Foi observada tendência de declínio estatisticamente significativo em todos os coeficientes analisados (-4,5%/ano na mortalidade geral, -3,6%/ano na neonatal precoce, -1,9%/ano no componente neonatal tardio e de -6,3%/ano no componente pós-neonatal). Adicionalmente, 64,3% dos municípios (n=119) apresentaram tendência de declínio no coeficiente de mortalidade infantil geral. Menos de 50% dos municípios apresentaram tendência de redução na mortalidade neonatal precoce e neonatal tardia (41,08%; n=76 e 43,2%; n=80, respectivamente). Na mortalidade pós-neonatal, 57,3% (n=106) apresentaram tendências decrescentes. Conclusões: houve tendência temporal de declínio da mortalidade infantil geral, embora haja importante parcela de municípios com tendência estacionária, justificando a necessidade de reduzir desigualdades sociais e assimetrias geográficas. Objetivos 200 2019 Métodos estado Vivos espaciais Resultados 47949 47 949 47.94 510 51 0 51,0 n=24.447 n24447 n 24 447 (n=24.447 vida 4,5%/ano 45ano 4 5 geral 3,6%/ano 36ano 3 6 1,9%/ano 19ano 1 9 6,3%/ano 63ano pósneonatal. pósneonatal pós . pós-neonatal) Adicionalmente 643 64 64,3 n=119 n119 119 (n=119 50 41,08% 4108 41 08 (41,08% n76 76 n=7 43,2% 432 43 2 n80 80 n=80 respectivamente. respectivamente respectivamente) pósneonatal, neonatal, pós-neonatal 573 57 57,3 n=106 n106 106 (n=106 decrescentes Conclusões estacionária geográficas 20 201 4794 94 47.9 51, n=24.44 n2444 44 (n=24.44 64, n=11 n11 11 (n=11 41,08 410 (41,08 n7 7 n= 43,2 n8 8 n=8 57, n=10 n10 10 (n=10 479 47. n=24.4 n244 (n=24.4 n=1 n1 (n=1 41,0 (41,0 43, n=24. n24 (n=24. (n= 41, (41, n=24 n2 (n=24 (n (41 n=2 (n=2 (4 (

Abstract Objectives: to analyze the temporal trend and spatial distribution of infant mortality in Pernambuco from 2001 to 2019. Methods: an ecological study involving all deaths in children under one year of age living in the state. Data were collected from the Mortality Information System and the Live Birth Information System. Four mortality rates were calculated and the temporal analysis was performed by applying the regression model by inflection points of the rates at different spatial levels. Results: 47,949 deaths were recorded, of which 51.0% (n=24,447) occurred in the first six days of life. A statistically significant downward trend was observed in all the rates analyzed (-4.5%/year in overall mortality, -3.6%/year in early neonatal mortality, -1.9%/year in the late neonatal component, and -6.3%/year in the post-neonatal component). Additionally, 64.3% of the municipalities (n=119) showed a declining trend in the overall infant mortality rate. Less than 50% of the municipalities showed a downward trend in early neonatal and late neonatal mortality (41.08%; n=76 and 43.2%; n=80, respectively). In post-neonatal mortality, 57.3% (n=106) showed decreasing trends. Conclusions: there was a temporal trend of decline in overall infant mortality, although there is an important portion of municipalities with a stationary trend, justifying the need to reduce social inequalities and geographical asymmetries. Objectives 200 2019 Methods state levels Results 47949 47 949 47,94 recorded 510 51 0 51.0 n=24,447 n24447 n 24 447 (n=24,447 life 4.5%/year 45year 4 5 3.6%/year 36year 3 6 1.9%/year 19year 1 9 component 6.3%/year 63year postneonatal post component. . component) Additionally 643 64 64.3 n=119 n119 119 (n=119 rate 50 41.08% 4108 41 08 (41.08% n76 76 n=7 43.2% 432 43 2 n80 80 n=80 respectively. respectively respectively) 573 57 57.3 n=106 n106 106 (n=106 trends Conclusions asymmetries 20 201 4794 94 47,9 51. n=24,44 n2444 44 (n=24,44 64. n=11 n11 11 (n=11 41.08 410 (41.08 n7 7 n= 43.2 n8 8 n=8 57. n=10 n10 10 (n=10 479 47, n=24,4 n244 (n=24,4 n=1 n1 (n=1 41.0 (41.0 43. n=24, n24 (n=24, (n= 41. (41. n=24 n2 (n=24 (n (41 n=2 (n=2 (4 (

Resumo Objetivos: avaliar o uso do suplemento de ferro e fatores associados em crianças de seis a 59 meses atendidas em unidades Estratégia Saúde da Família em município de Minas Gerais (MG). Métodos: estudo transversal, analítico-exploratório, realizado com 252 crianças na faixa etária seis a 59 meses, atendidas em dez unidades, entre 2014 e 2016. Utilizou-se questionário estruturado para coleta de dados sobre condições socioeconômicas, saúde materna, saúde da criança e conhecimentos dos responsáveis acerca da anemia e suplementação com ferro, além de análise do prontuário / caderneta da criança para coleta de resultados de exames. Realizou-se análise estatística descritiva, análise de associação bivariada e regressão logística para análise multivariada. Resultados: apenas 22,6% das crianças com idade entre seis e 24 meses estavam recebendo o suplemento de ferro no momento da entrevista. Considerando as crianças de seis a 59 meses, 13,1% eram suplementadas e o tempo de amamentação (p=0,006) e a participação na puericultura (p=0,042) apresentaram associação com o uso de suplemento. Conclusão: a maioria das crianças de seis a 24 meses não recebia a suplementação, demonstrando a necessidade de implementação dos programas de prevenção, como o Programa Nacional de Suplementação de Ferro e capacitação dos profissionais sobre a importância da educação em saúde para prevenção da anemia infantil. Objetivos 5 MG. MG . (MG) Métodos transversal analíticoexploratório, analíticoexploratório analítico exploratório, exploratório analítico-exploratório 25 201 2016 Utilizouse Utilizou se socioeconômicas materna exames Realizouse Realizou descritiva multivariada Resultados 226 22 6 22,6 2 entrevista 131 13 1 13,1 p=0,006 p0006 p 0 006 (p=0,006 p=0,042 p0042 042 (p=0,042 Conclusão infantil (MG 20 22, 13, p=0,00 p000 00 (p=0,00 p=0,04 p004 04 (p=0,04 p=0,0 p00 (p=0,0 p=0, p0 (p=0, p=0 (p=0 p= (p= (p

Abstract Objectives: to evaluate the use of iron supplementation and associated factors in children aged six to 59 months attended at the Family Health Strategy units in a city in Minas Gerais (MG). Methods: a cross-sectional, analytical-exploratory study, carried out with 252 children aged six to 59 months, attended at ten units, between 2014 and 2016. A structured questionnaire was used to collect data on socioeconomic conditions, maternal health, child’s health and the knowledge of those responsible regarding anemia and iron supplementation, in addition to analysis of the child’s medical records /booklet to collect test results. Descriptive statistics, bivariate association analysis and logistic regression for multivariate analysis were performed. Results: only 22.6% of the children aged between six and 24 months were receiving iron supplement at the time of the interview. Considering children aged six to 59 months, 13.1% were supplemented and breastfeeding time (p=0.006) and the participation in childcare (p=0.042), were positively associated with the use of supplementation. Conclusion: most children aged six to 24 months were not receiving supplementation as recommended by the Ministry of Health, demonstrating the need to implement prevention programs, such as the National Iron Supplementation Program and training of professionals on the importance of health education in preventing childhood anemia. Objectives 5 MG. MG . (MG) Methods crosssectional, crosssectional cross sectional, sectional cross-sectional analyticalexploratory analytical exploratory study 25 201 2016 conditions childs child s booklet results statistics performed Results 226 22 6 22.6 2 interview 131 13 1 13.1 p=0.006 p0006 p 0 006 (p=0.006 p=0.042, p0042 p=0.042 , 042 (p=0.042) Conclusion programs (MG 20 22. 13. p=0.00 p000 00 (p=0.00 p004 p=0.04 04 (p=0.042 p=0.0 p00 (p=0.0 (p=0.04 p=0. p0 (p=0. p=0 (p=0 p= (p= (p

Abstract The aim of this study was to evaluate the microbiological quality of Minas fresh cheese commercialized in Federal District, Brazil. For this, 20 samples of industrialized cheeses were collected in supermarkets and 20 samples of artisanal cheeses were collected in public food markets. The analyzes performed were total count of bacteria, determination of coliforms, count of S. aureus and identification of Salmonella spp. (InvA gene) and S. aureus (SeC gene). The results showed that 26 samples (65%) were unfit for consumption according to Brazilian legislation. For industrialized cheeses, 6 samples (15%) of were unfit for consumption (3 samples for excess of thermotolerant coliforms and 3 samples for excess of S. aureus). For artisanal cheeses, all 20 samples (50%) were unfit for consumption due to the high S. aureus counts, including 6 samples that had excess thermotolerant coliforms and 1 sample that was contaminated with Salmonella. Comparing the results, industrialized cheeses showed better microbiological quality than artisanal cheeses. Thus, artisanal Minas fresh cheeses commercialized in the public food markets of the Federal District showed the presence of bacterial hazards, and there is an evident need for good hygiene practices in the entire production chain to ensure consumer food safety.

Resumo Este artigo trabalha o hibridismo entre a comunicação política partidária digital e o jornalismo audiovisual. A proposta é relacionar a atividade comunicativa na rede social Instagram de seis partidos portugueses com representação parlamentar (PS, PSD, BE, PCP, CDS e Chega), e dos respetivos líderes, com a cobertura jornalística da sua atividade realizada pelos três principais noticiários televisivos, respetivamente o Jornal da Noite (SIC), o Telejornal (RTP1), o Jornal das 8 (TVI) e o CM Jornal (CMTV). Analisamos a comunicação política e partidária na rede social e os enquadramentos noticiosos no jornalismo televisivo, identificando padrões na relação entre as duas plataformas. Exploramos a contaminação entre os discursos textuais e visuais dos vários partidos e os noticiários televisivos, relevando as principais estratégias retóricas ativadas no Instagram e as narrativas televisivas. O artigo pretende contribuir para uma melhor compreensão das lógicas comunicativas dos partidos em ambiente digital e explicitar as transformações na cobertura da política pelos noticiários televisivos, em sistemas mediáticos híbridos.

Abstract This article focuses on the hybridism between political parties digital communication and audiovisual journalism. It proposes to relate the communicative activity in the Instagram social network of six Portuguese parties with parliamentary representation (PS, PSD, BE, PCP, CDS and Chega), and their respective leaders, with the journalistic coverage of their activity carried out by the three main TV news, respectively Jornal da Noite (SIC), Telejornal (RTP1), and Jornal das 8 (TVI). We analyzed the political and partisan communication in the social network and the news frameworks in television journalism, identifying patterns in the relationship between the two platforms. We explored the cross contamination between the textual and visual discourses of the various parties and the television news, highlighting the main rhetorical strategies activated in Instagram and the television narratives. The article aims to contribute to a better understanding of the communicative logics of the parties in a digital environment and to explicit the transformations in the coverage of politics by television newspapers, in hybrid media systems.

RESUMO A utilização de alimentos alternativos para coelhos visa reduzir os custos de produção, devido sua habilidade em aproveitar os nutrientes de alimentos não-convencionais. Desse modo, a casca do maracujá (CM) surge como potencial de utilização na alimentação de coelhos. Objetivou-se determinar a composição química e energética da CM e avaliar os efeitos da inclusão para coelhos em crescimento. No ensaio de digestibilidade foram utilizados 24 coelhos distribuídos entre dois tratamentos (ração referência e ração teste, composta por 70% de ração referência e 30% de CM) com 12 repetições de um animal por parcela. No ensaio de desempenho foram utilizados 120 coelhos, distribuídos em delineamento de blocos ao acaso, em arranjo fatorial 5x2, sendo 5 níveis de inclusão de CM (0, 8, 16, 24, 32%) e 2 sexos (machos e fêmeas) correspondendo ao total de 10 tratamentos com 12 repetições cada. A CM apresentou 10,99% PB, 6,25% EE, 47,40% FDA, 54,66% FDN e 4.156,50 kcal de ED/kg de matéria seca. A inclusão da CM não influenciou o peso final e o ganho de peso dos coelhos, no entanto observou-se que os coelhos alimentados ao nível de 32% de inclusão consumiram menor quantidade de ração, sem efeito negativo sobre o desempenho. Observou-se diminuição dos níveis de colesterol e triglicerídeos em relação ao controle e um aumento da creatinina no sangue dos coelhos. Como houve melhora nos parâmetros econômicos sem prejudicar o desempenho e características de carcaça, recomenda-se a inclusão deste ingrediente até o nível de 32% em rações para coelhos em crescimento.

ABSTRACT The use of alternative foods for rabbits aims to reduce production costs, due to their ability to extracting nutrients from non-conventional feeds. Thus, passion fruit peel (PFP) appears as a potential use in feeding rabbits. This study aimed to determine the chemical and energetic composition of PFP and evaluate the effects of inclusion for growing rabbits. For the digestibility trial, 24 rabbits were distributed between two treatments (reference ration and test ration, composed of 70% reference ration and 30% PFP) with 12 replications of one animal per plot. For the performance trial, 120 rabbits were distributed within a randomized block design, in a 5x2 factorial arrangement, with 5 levels of inclusion of PFP (0, 8, 16, 24, 32%) and 2 sexes (male and female) corresponding to a total of 10 treatments with 12 replications each. PFP had 10.99% CP, 6.25% EE, 47.40% ADF, 54.66% NDF and 4,156.50 kcal DE/kg, on a dry matter. The inclusion of PFP did not influence the final weight and weight gain of rabbits, however it was observed that rabbits fed at the level of 32% inclusion of PFP consumed less amount of feed, with no negative effect on performance. Decreased cholesterol and triglyceride levels were observed in relation to control and an increase of creatinine in rabbit's blood. As there was an improvement in the economic parameters without affecting the performance and carcass characteristics, it’s recommended to include this ingredient up to level of 32% in the feed for growing rabbits.

RESUMO: Com o objetivo de padronizar avaliações da severidade da antracnose em frutos de feijoa elaborou-se e validou-se uma escala diagramática com os seguintes níveis de severidade: 2, 10, 20, 40, 70 e 100% de área superficial do fruto com lesão. A escala considerou os limites de severidade mínima e máxima da doença observados em campo e os níveis intermediários seguiram incrementos logarítmicos, obedecendo-se a “Lei do estímulo de Weber-Fechner”. A severidade foi estimada, por oito avaliadores sem experiência, em 50 frutos sem e com o auxílio da escala proposta. Em conclusão, a escala melhorou a avaliação da antracnose na feijoa. Oito genótipos de diferentes cruzamentos foram tolerantes à antracnose.

ABSTRACT: A diagrammatic scale of anthracnose in feijoa fruit was elaborated and validated in order to standardize disease severity assessments. The proposed scale showed six disease severity levels: 2, 10, 20, 40, 70 and 100% of the injured fruit surface. The scale took into account the minimum and maximum limits of disease severity observed in the field and the intermediate values followed logarithmic increments according to the Weber-Fechner stimulus-response law. Eight inexperienced raters validated the scale by quantifying the disease severity (using/not using the scale) of 50 feijoas with anthracnose symptoms. In conclusion, the scale improved the assessment of anthracnose in feijoa. Eight genotypes from different crosses were tolerant to anthracnose.

ABSTRACT Background: Oral nutritional supplements aim at offsetting dietary deficits and helping to meet energy and protein targets. Due to the absence of data about their role in food intake, it is necessary to evaluate the contribution of these products to the estimated needs of hospitalized patients. Methods: This is a prospective longitudinal study with hospitalized patients over 18 years of age, admitted to a public university hospital, who were given oral nutritional supplements, according to clinical guidelines. Food intake was quantified throughout the period such products were prescribed. Results: 805-day food intake follow-up and record of 128 patients, who took supplements for 6.4 days on average. The use of nutritional supplements demonstrated a significant contribution to the input of both energy (1576 kcal/day versus 1263 kcal/day, p<0.001) and protein (68.7 g/day versus 50.3 g/day, p<0.001). Conclusions: Nutritional supplements help increase food intake and achieve nutritional goals when prescribed in a hospital setting.

RESUMEN Antecedentes: los suplementos nutricionales orales tienen como objetivo compensar los déficits dietéticos y ayudar a cumplir los objetivos de energía y proteínas. Debido a la ausencia de datos sobre su papel en la ingesta de alimentos, es necesario evaluar la contribución de estos productos a las necesidades estimadas de los pacientes hospitalizados. Métodos: Este es un estudio longitudinal prospectivo con pacientes hospitalizados mayores de 18 años, ingresados en un hospital universitario público, que recibieron suplementos nutricionales orales, según la derivación clínica. La ingesta de alimentos se cuantificó durante todo el período en que se prescribieron dichos productos. Resultados: seguimiento de la ingesta alimentaria de 805 días y registro de 128 pacientes, que tomaron suplementos durante 6,4 días en promedio. El uso de suplementos nutricionales demostró una contribución significativa al aporte de energía (1576 kcal/día versus 1263 kcal/día, p< 0,001) y proteína (68,7 g/día versus 50,3 g/día, p<0,001). Conclusiones: los suplementos nutricionales ayudan a aumentar la ingesta de alimentos y alcanzar objetivos nutricionales cuando se prescriben en un entorno hospitalario.

Resumo Dois ensaios foram realizados: (1) para determinar a composição química, digestibilidade dos nutrientes e energia do farelo de castanha de caju (FCC) e avaliar os efeitos do aumento dos níveis (0, 5, 10, 15, 20 e 25%) em dietas para coelhos em crescimento sobre desempenho, características de carcaça e avaliação econômica. O ensaio de digestibilidade utilizou 24 coelhos (55 dias de idade), distribuídos em delineamento inteiramente casualizado, com dois tratamentos e 12 repetições, sendo uma ração referência e outra ração teste composta por 70% de dieta basal e 30% de FCC. A digestibilidade da matéria seca, proteína bruta e energia bruta do FCC foram, respectivamente, 76,61; 61,71 e 56,53%. A matéria seca digestível, a proteína digestível e a energia digestível foram, respectivamente, 74,28; 16,97 e 3,549 kcal / kg. O ensaio de desempenho utilizou 120 coelhos, com média de 45 dias de idade e peso de 1090 ± 151g, distribuídos em delineamento inteiramente casualizado, com seis tratamentos e 10 repetições com dois coelhos do mesmo sexo por gaiola. A inclusão de FCC acima de 5% promoveu uma redução linear no consumo de ração e melhor conversão alimentar, sem afetar as características de ganho de peso e carcaça para o nível de inclusão de 20%. Também foi observada redução linear no custo de alimentação por quilograma de ganho de peso e melhora linear nas taxas de eficiência econômica e índice de custos até 25% de inclusão do FCC. Com base nesses resultados - e para não prejudicar o desempenho - é recomendável incluir até 20% de FCC na dieta de coelhos.

Abstract Two trials were conducted: (1) to determine the chemical composition, digestibility of nutrients and energy of cashew nut meal (CNM); and (2) to evaluate the effects of increasing its levels (0, 5, 10, 15, 20 and 25%) in diets for growing rabbits on performance, carcass characteristics and economic evaluation. The digestibility assay utilized 24 rabbits (55 days of age), distributed in a completely randomized design with two treatments and 12 repetitions, with one reference and another test feed composed of 70% basal diet and 30% CNM. The digestibility of dry matter, crude protein and gross energy from CNM were, respectively, 76.61, 61.71 and 56.53%. The digestible dry matter, digestible protein and digestible energy were, respectively, 74.28, 16.97 and 3,549 kcal/kg. The performance assay utilized 120 rabbits, with an average of 45 days of age and weight 1090 ± 151g, distributed in a completely randomized design with six treatments and 10 repetitions with two rabbits of the same sex per cage. The inclusion of CNM above 5% promoted a linear reduction in feed intake and improved feed conversion, without affecting weight gain and carcass characteristics to the 20% level of inclusion. There was also a linear reduction in feed cost per kilogram of weight gain and linear improvement in rates of economic efficiency and cost index up to 25% CNM inclusion. Based on these findings - and to not hurt performance - it is recommended to include up to 20% CNM in rabbits' diet.

Equal channel angular pressing (ECAP) is one of the severe plastic deformation processes that can also be used for metallic powder consolidation. Consolidation of blended elemental powders of iron, chromium, nickel, and manganese (Fe-25Cr-20Ni-2Mn wt. %) was performed at room temperature in a Φ = 120° die by 1 and 2 passes. SEM micrographs indicated that single pass ECAP consolidated sample presented close to full densification. Additional pass of ECAP led to hardness increase and to an apparent better mixing between the different particles. SEM/EDX analysis made before and after heat treatment of the samples showed that effective diffusion only took place after heat treatment and especially in the sample subjected to 2 ECAP passes. Results indicate that alloying by ECAP consolidation and posterior heat treatment is feasible, especially for systems that cannot be processed by conventional means, as well as mechanical alloying.

Abstract Rubber blends are important materials in automotive industry, as well as in other sectors. However, there are implications when suitable use of a polymer in an artifact is not made. In the automotive area, for example, the use of an elastomer without the fuel resistance requirement would result in component degradation, potential fuel leakage, and danger of fire. The use of polymer blends may be the solution to this problem. Fourier transform infrared spectroscopy (FT-IR) can be used for the knowledge of the polymer content of these blends. Then, FT-IR quantitative methodologies for determining acrylonitrile-butadiene copolymer (NBR) copolymer and butadiene-styrene copolymer (SBR) contents were developed by the transflectance accessory, NIRA, and the transmission mode, being the sample analyzed by transmission and universal attenuated total reflection (UATR) in the medium infrared (MIR). UATR and NIRA methodologies showed better accuracy. However, the MIR analysis showed a detection limit between 10-20% of NBR.

Abstract When using Fourier transform infrared spectroscopy (FT-IR) under the qualitative aspect for characterization of polymer blends, often a simple identification of each one of the existing polymers in the blend does not justify the material failure since different amounts of each component may result in different mechanical properties, which should be a possible cause for of material failure when applied to an automotive part. Thus, seeking for a better justification in the understanding of material failure analysis, a new quantitative FT-IR methodology was developed in the mid-infrared region (MIR), using the transmission techniques and universal attenuated reflectance for the determination of Acrylonitrile Butadiene Styrene (ABS) and polycarbonate (PC). Transmission mode was more suitable. The relative band (A831 /A2237) was chosen for the preparation of the calibration curve that showed a 0.99% error methodology, which is within the FT-IR spectrometer accuracy limit (≤ 2%); therefore, it is accurate for the analysis of the system.

RESUMO: Apesar da reconhecida importância de Clostridium perfringens como enteropatógeno de pequenos ruminantes, pouco se sabe sobre a frequência dos genótipos ou do papel de fatores de virulência adicionais de C. perfringens em cabras saudáveis, dificultando o diagnóstico laboratorial da infecção causada por esse micro-organismo. Dessa forma, o presente estudo teve como objetivo caracterizar C. perfringens de amostras de fezes de cabras adultas saudáveis. Amostras de fezes de 250 cabras saudáveis de 17 rebanhos diferentes em Minas Gerais, Brasil, foram submetidas ao isolamento e genotipagem de C. perfringens. C. perfringens tipo A foi isolado de 189 (75,6%) cabras, enquanto C. perfringens tipos C e D foram detectados em um animal (0.4%) cada. Todos os isolados foram negativos para os genes codificadores das toxinas NetB, NetE, NetF e enterotoxina. Os resultados apresentados confirmam C. perfringens tipo A como parte da microbiota de cabras saudáveis e sugere que C. perfringens tipos C e D são raramente encontrados em caprinos saudáveis.

ABSTRACT: Despite the known importance of Clostridium perfringens as an enteropathogen in small ruminants, little is known about the role of its additional virulence factors or the frequency of the various C. perfringens genotypes in healthy goats; this complicates the laboratory diagnosis of the infections caused by this microorganism. In light of this, the aim of the present study was to isolate and genotype C. perfringens from stool samples from healthy goats in Brazil. Stool samples from 250 apparently healthy adult goats from 17 different herds in Minas Gerais, Brazil were collected, and isolation and genotyping of C. perfringens was performed. C. perfringens type A was isolated from 189 (75.6%) goats, whereas C. perfringens types C and D were each detected in one goat (0.4%). All isolates were negative for enterotoxin-, NetB-, NetE-, and NetF-encoding genes. These results confirmed C. perfringens type A as part of the microbiota in these animals, and they suggested that C. perfringens type C and D are rarely isolated from healthy goats.