Resumo O objetivo deste estudo foi avaliar o efeito de soluções de imersão ou de um método in vivo na alteração volumétrica dos materiais biocerâmicos reparadores Bio-C Repair (BCR, Angelus, Londrina, PR, Brasil) e Biodentine (BIO, Septodont, Saint-Maur-des-Fossés, França) em comparação com IRM (Dentsply Sirona, York, Pensilvânia, EUA), usando a avaliação por microtomografia computadorizada (µCT). Tubos de cloreto de polivinila (PVC, 4 mm de comprimento x 1,3 mm de diâmetro interno, n = 7) foram preenchidos com os materiais para análise volumétrica no µCT. As amostras foram escaneadas após a presa dos materiais e após a imersão em água destilada, PBS ou no tecido subcutâneo de ratos por 7 dias. O IRM apresentou uma alteração volumétrica maior do que o BCR e BIO em todas as soluções de imersão (P <0,05). BIO e BCR apresentaram uma alteração volumétrica semelhante quando imersos em PBS e água destilada (P> 0,05). Quando o método in vivo foi usado, BIO e BCR apresentaram uma menor alteração volumétrica (P <0,05), incluindo um aumento no volume para BCR. As soluções de imersão influenciaram a avaliação da alteração volumétrica dos materiais biocerâmicos reparadores. Os materiais biocerâmicos mostram maior estabilidade volumétrica quando avaliados pelo método in vivo. O método in vivo no tecido subcutâneo de ratos pode ser uma alternativa para analisar as propriedades de cimentos biocerâmicos, mostrando semelhança com a aplicação clínica. BioC Bio C BCR, (BCR Angelus Londrina PR Brasil BIO, (BIO Septodont SaintMaurdesFossés, SaintMaurdesFossés Saint Maur des Fossés, Fossés Saint-Maur-des-Fossés França Dentsply Sirona York Pensilvânia EUA, EUA , EUA) µCT . (µCT) PVC, PVC (PVC 13 1 3 1, interno dias P <0,05. 005 <0,05 0 05 <0,05) P> 0,05. 0,05 0,05) usado <0,05, clínica (µCT 00 <0,0 0,0 <0, 0, <0 <

Abstract This study aimed to evaluate the effect of in vitro immersion solutions or an in vivo method on volumetric change of bioceramic root repair materials: Bio-C Repair (BCR, Angelus, Londrina, PR, Brazil) and Biodentine (BIO, Septodont, Saint-Maur-des-Fossés, France) compared to IRM (Dentsply Sirona, York, Pennsylvania, USA) by using microcomputed tomography (µCT) assessment. Tubes of polyvinyl chloride (PVC, 4 mm of length x 1.3 mm of inside diameter, n = 7) were filled with the materials for volumetric analysis in µCT. Samples were scanned after materials setting and after immersion in distilled water, PBS, or in vivo tissue fluid of subcutaneous tissue of rats for 7 days. IRM showed higher volumetric change than BCR and BIO in all immersion solutions (P<0.05). BIO and BCR presented similar volumetric changes when immersed in PBS and distilled water (P>0.05). When the in vivo method was used, BIO and BCR showed lower volumetric change (P<0.05), including an increase in volume for BCR. The immersion solutions influenced the evaluation of the volumetric change of bioceramic repair materials. Bioceramic materials show greater volumetric stability when evaluated by the in vivo method. The in vivo method in the subcutaneous tissue of rats can be an alternative for analyzing the properties of bioceramic cement, showing similarity with the clinical application. BioC Bio C BCR, (BCR Angelus Londrina PR Brazil BIO, (BIO Septodont SaintMaurdesFossés, SaintMaurdesFossés Saint Maur des Fossés, Fossés Saint-Maur-des-Fossés France Dentsply Sirona York Pennsylvania USA µCT (µCT assessment PVC, PVC (PVC 13 1 3 1. diameter days P<0.05. P005 P P<0.05 . 0 05 (P<0.05) P>0.05. P>0.05 (P>0.05) used P<0.05, , cement application P00 P<0.0 (P<0.05 P>0.0 (P>0.05 P0 P<0. (P<0.0 P>0. (P>0.0 P<0 (P<0. P>0 (P>0. P< (P<0 P> (P>0 (P< (P> (P

Abstract This systematic review aims to provide preclinical evidence of the antimicrobial efficacy of natural endodontic solutions (NES) compared to sodium hypochlorite (NaOCl) and chlorhexidine (CHX) against Enterococcus faecalis. The study followed the PRISMA guidelines and had a registered protocol (PROSPERO – CRD42021224022). The inclusion criteria comprised ex vivo studies simulating root canal irrigation to assess the standardized mean difference of colony-forming units (CFUs). Records were systematically identified in PubMed, Scopus, EMBASE, SciELO, Web of Science, LILACS, DANS Easy, and BDTD databases in January 2024. The RoBDEMAT tool helped determine the risk of bias. As for the network meta-analysis, CFU data were collected pre- and post-irrigation protocols as an effect measure. Thirty-five studies met the eligibility criteria and were included in the qualitative analysis, and seven studies were eligible for the network meta-analysis. The total sample consisted of 1,504 root canals. Among the tested solutions, apple cider vinegar combined with 2.5% NaOCl showed the highest probability of reducing CFUs after root canal irrigation (95%CI: -0.76–0.20), followed by 2% CHX and NaOCl (considering the different concentrations [95%CI: -0.32–0.17]). The bias assessment revealed significant omissions in reporting, particularly regarding sample size calculation, sample randomization, and operator blinding. The obtained evidence demonstrated that, even though NES did not overcome NaOCl, they have antimicrobial potential against Enterococcus faecalis. (NES (NaOCl (CHX faecalis PROSPERO CRD42021224022. CRD42021224022 CRD . CRD42021224022) colonyforming colony forming CFUs. (CFUs) PubMed Scopus EMBASE SciELO Science LILACS Easy 2024 metaanalysis, metaanalysis meta analysis meta-analysis pre postirrigation post measure Thirtyfive Thirty five metaanalysis. analysis. 1504 1 504 1,50 canals 25 2 5 2.5 95%CI 95CI CI 95 (95%CI 0.76–0.20, 076020 0.76–0.20 , 0 76 20 -0.76–0.20) considering [95%CI 0.32–0.17. 032017 0.32–0.17 32 17 -0.32–0.17]) reporting calculation randomization blinding that CRD4202122402 (CFUs 202 150 50 1,5 2. 9 07602 0.76–0.2 7 -0.76–0.20 03201 0.32–0.1 3 -0.32–0.17] CRD420212240 15 1, 0760 0.76–0. -0.76–0.2 0320 0.32–0. -0.32–0.17 CRD42021224 076 0.76–0 -0.76–0. 032 0.32–0 -0.32–0.1 CRD4202122 07 0.76– -0.76–0 03 0.32– -0.32–0. CRD420212 0.76 -0.76– 0.32 -0.32–0 CRD42021 0.7 -0.76 0.3 -0.32– CRD4202 0. -0.7 -0.32 CRD420 -0. -0.3 CRD42 -0 CRD4 -

Abstract: Solubility, pH, ion release, cytotoxicity, and osteoclastogenesis inhibition in bone marrow-derived monocyte macrophages (BMMs) were evaluated in EndoSequence BC Sealer (END), Bio-C Sealer (BC), and Sealer Plus BC (SPBC). pH was determined after immersion of the sealers in deionized water (DW) and Minimum Essential Medium Alpha (α-MEM). Solubility was obtained by mass loss. Ion release was measured by using X-ray fluorescence spectroscopy (XRF). Cytotoxicity was evaluated by MTT assay. Inhibition of osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP). Data were analyzed using the t-test, ANOVA and Tukey/Dunnett's post-hoc tests (α = 0.05). END had the highest pH in DW (p < 0.05), and BC, in α-MEM (p < 0.05). Solubility in DW was the lowest for SPBC (p < 0.005). The highest calcium release was observed for BC in DW at 12 h (p < 0.05), and in α-MEM at 12 and 24 h (p < 0.05). The lowest toxicity was detected for END (p < 0.05). BC had the highest inhibitory effect on osteoclasts (p < 0.05). Overall, the highest solubility and pH values were found in DW. However, the calcium silicate-based sealer showed higher solubility than the ISO standards. Calcium release was the highest for BC. END showed the highest cell viability, and BC, the highest osteoclast inhibition.

Abstract: The aim of this study was to investigate the effects of different post space irrigation protocols for removing residual filling material from dentin walls, by using microcomputed tomography (micro-CT), and the influence of these protocols on dentin microhardness. Bovine incisors (n = 35) were filled with the single-cone technique and MTA Fillapex (Angelus, Londrina, PR, Brazil). Post space preparation (PSP) was performed 7 days after filling, using the Odous Touch electrical system (Odous De Deus Ind. e Com., Belo Horizonte, MG, Brazil), followed by post space irrigation using manual irrigation, passive ultrasonic irrigation, or Easy Clean, together with 2.5% sodium hypochlorite (NaOCl), or with 2.5% NaOCl and 17% EDTA (NaOCl/EDTA). Micro-CT scans were performed at three time points. The residual filling material was evaluated at three levels: cervical, middle and apical. The Knoop test was measured with four indentations around the canal lumen at three dentin depths: X (100 μm), Y (200 μm) and Z (400 μm). Statistical analysis was performed using ANOVA (p < 0.05). The effects of the activation method (p < 0.001), and the root level (p = 0.013), as well as the interaction between the irrigant and the activation method (p = 0.041), led to different percentages of residual filling material. Lower amounts of residual filling material were observed at the cervical versus the middle and apical levels (p < 0.05). No significant differences were observed in dentin microhardness (p > 0.05). The best removal of the residual filling material was performed using the Easy Clean tip and NaOCl/EDTA, regardless of the activation methods.

Resumo O objetivo deste estudo foi avaliar a presença de resíduos após o alívio do canal (AC) e verificar se o deslocamento apical da guta-percha foi afetado pelos momentos e métodos de AC em dentes obturados pela técnica do cone único. Os canais radiculares de 20 dentes incisivos bovinos foram instrumentados com Reciproc e obturados com cone único e AH Plus. As amostras foram divididas em 4 grupos de acordo com o momento e método de AC (n=5): broca imediato, termocompactador imediato, broca tardio e termocompactador tardio. Escaneamentos em micro-CT foram realizados antes e após o AC para análise dos resíduos da obturação (1) e do deslocamento apical (2). Os dados foram analisados empregando ANOVA em 2 fatores com medida repetida (1), ANOVA em dois fatores (2), seguidas do teste de Tukey (α=0,05). A porcentagem de resíduos remanescentes foi influenciada pelos métodos (p=0,044), momentos (p=0,006), pelo fator de repetição terços do canal (p<0,001), e pelas interações entre métodos e terços (p<0,001), e momentos e terços (p=0,044). Já para o deslocamento apical da obturação do canal radicular foi detectada influência significativa para os métodos (p=0,008), no entanto, nenhuma diferença foi encontrada entre os momentos (p=0,617). Em geral, AC usando brocas resultou em preparo mais homogêneo dos canais radiculares, principalmente quando realizado imediatamente após a obturação. Para todas as outras combinações entre métodos e momentos de AC, o terço médio e apical apresentaram significativamente maiores resíduos remanescentes. O método termo realizado nos dois momentos e o método broca de preparo realizado imediatamente resultaram em deslocamento na direção apical, representando a extrusão do material de obturação no canal radicular.

Abstract The purpose of this study was to evaluate the presence of residues after post space preparation (PSP) and establish whether the apical displacement of the gutta-percha was affected by the moments and methods of PSP in teeth filled by the single-cone technique. The root canals of 20 bovine incisor teeth were instrumented with Reciproc and filled with single-cone and AH Plus. The specimens were divided into 4 groups according to the moment and method of PSP (n=5): immediate with drill, immediate with thermoplasticizer, delayed with drill and delayed with thermoplasticizer. Micro-CT scans were performed before and after the PSP for residues of the root canal filling (1) and analysis of apical displacement (2). Data were analyzed by using 2-way repeated measurement ANOVA (1) and 2-way ANOVA (2) followed by the Tukey’s test (a=0.05). Significance effect on the residues percentage remaining for methods (p=0.044), for moments (p=0.006), for thirds repetition (p<0.001), and for interaction between methods and thirds (p<0.001), and moments and thirds (p=0.044). Significance effect on the apical displacement of root canal filing was detected for methods (p=0.008), however no difference was found between moments (p=0.617). In general, PSP using drill resulted in more homogeneous root canal preparation, mainly when made immediately. For all other combinations between methods and moments for PSP, the middle and apical thirds presented significant higher residues remaining. Thermo method performed in both moments and the drill method performed immediately had displacement in the apical direction, representing extrusion of the root filling material.