This study details the preparation, antileishmanial assay, and in silico analysis of twenty 2-hydroxy-3-(1,2,3-triazolyl)propyl vanillin derivatives. These compounds were synthesized in three steps and evaluated against Leishmania infantum, Leishmania amazonensis, and Leishmania braziliensis promastigotes. Compounds 3s and 3t were the most effective, showing good activity against all Leishmania species tested. Molecular docking indicated that all compounds bind favorably to the sterol 14α-demethylase (CYP51) enzyme from L. infantum. ADMET (absorption, distribution, metabolism, excretion and toxicity) analysis indicated good oral bioavailability, non blood-brain barrier penetration, and high gastrointestinal absorption. Posaconazole and compounds 3e, 3s, and 3t remained stable in the CYP51 binding region during 100 ns molecular dynamics (MD) simulations. Root mean square deviation (RMSD) and root mean square fluctuations (RMSF) analyses from the MD trajectory revealed significant conformational fluctuations of the CYP51 N-terminal, suggesting occasional expulsion of 3e, potentially explaining its higher IC50 (half-maximal inhibitory concentration) values. Pairwise decomposition analyses from molecular mechanics Poisson-Boltzmann surface area (MM/PBSA) calculations highlighted the importance of hydrophobic residues in interacting with the synthesized derivatives. preparation assay 2hydroxy31,2,3triazolylpropyl 2hydroxy3123triazolylpropyl hydroxytriazolylpropyl 2 hydroxy 3 1,2,3 triazolyl propyl 1 derivatives infantum amazonensis promastigotes s t effective tested 14αdemethylase αdemethylase 14α demethylase α CYP (CYP51 L absorption, absorption (absorption distribution metabolism toxicity bioavailability bloodbrain blood brain penetration 3e e CYP5 10 (MD simulations RMSD (RMSD RMSF (RMSF Nterminal, Nterminal N terminal, terminal N-terminal IC IC5 halfmaximal half maximal concentration values PoissonBoltzmann Poisson Boltzmann MM/PBSA MMPBSA MM PBSA (MM/PBSA 2hydroxy31 3triazolylpropyl triazolylpropyl 123 1,2, (CYP5 2hydroxy3 12 1,2 (CYP 2hydroxy 1,

Abstract The flotation mechanism of galena with xanthate as the collector is generally explained based on the mixed potential of the pulp, in which an anodic electron transfer reaction from the collector to the mineral is combined with an O2 cathodic reduction. Sodium sulphide, often used as a mineral activator, strongly influences the pulp's mixed potential, and depending on the dosage applied, it can become a galena depressor reagent. In this way, the current study assesses galena flotability in the absence of collector, as well as in the presence of the collector potassium amyl xanthate (PAX) and sodium sulphide. The effect of an extremely cathodic potential was also studied through the addition of hydrogen peroxide. In addition, the effect of different stages in the oxidation of the galena surface on the flotability was investigated. Moreover, the zeta potential was measured and contact angle (wettability) tests were carried out. The contact angle measurements were taken for a distilled water, PAX solution and for a mixture of PAX and sodium sulphide solution on the oxidized and non-oxidized surface of galena. All the results showed that galena could be floated in all the PAX concentrations tested. It was observed that a moderate oxidation in the galena surface may contribute to an improvement of its flotation with PAX. However, extremely anodic and cathodic potentials cause flotability close to zero. The zeta potential results showed that sodium sulphide affected the interaction between PAX and galena. The measurements of the contact angle corroborated the results obtained in the microflotation tests. pulp O reduction activator pulps s applied reagent way (PAX peroxide investigated Moreover wettability (wettability out water nonoxidized non tested However zero

Abstract Aim This study aims to analyze the scientific literature on phytoplankton in assessing lake water quality, based on bibliometric and network techniques. Methods PRISMA criteria were adopted to produce reliable results. The Scopus and Web of Science databases were consulted to retrieve the documents to be studied. The number of publications, citations and bibliographic coupling were techniques used to identify relevant journals, countries, authors, and articles. The conceptual evolution was analyzed by keywords co-occurrence and thematic mapping. Results Based on 2429 documents selected from the 1973-2023 annual period, the main results indicated 519 journals, 6450 authors, 54907 references, and 4844 keyword authors, among others. The annual growth index was 10.27%, reflecting the upward trend at the time. Erick Jeppesen resulted as the top influential author, China leaded in publications and collaborations with The United States of America. Hydrobiologia was the top journal. Top influential articles content theme related to cyanobacterial blooms. According to the results of the analysis of the conceptual framework, phytoplankton, water quality, eutrophication, and cyanobacteria were the most relevant themes. Furthermore, the trending topics were mainly climate change and degradation. Conclusions This comprehensive analysis allowed us to interpret the development of research related to the subject of assessing lake water quality. quality studied journals countries authors cooccurrence co occurrence mapping 242 19732023 1973 2023 1973-202 period 51 645 5490 references 484 others 1027 10 27 10.27% time author America journal blooms framework eutrophication themes Furthermore degradation 24 1973202 197 202 1973-20 5 64 549 48 102 1 2 10.27 197320 19 20 1973-2 6 54 4 10.2 19732 1973- 10.

Resumo Objetivo Este estudo teve como objetivo a análise da literatura científica sobre o fitoplâncton na avaliação da qualidade da água de lagos, baseada em técnicas bibliométricas e de rede. Métodos Critérios PRISMA foram adotados para produzir resultados confiáveis. As bases de dados Scopus e Web of Science foram consultadas para recuperação dos documentos a serem estudados. O número de publicações, citações e acoplamento bibliográfico foram técnicas utilizadas para identificar periódicos, países, autores e artigos relevantes. A evolução conceitual foi analisada por meio de coocorrência de palavras-chave e mapeamento temático. Resultados Com base em 2429 documentos selecionados do período anual 1973-2023, os principais resultados indicaram 519 periódicos, 6450 autores, 54907 referências e 4844 autores de palavras-chave, entre outros. O índice de crescimento anual foi de 10.27%, refletindo a tendência ascendente da época. Erick Jeppesen foi apontado como o autor mais influente, a China liderou em publicações e colaborações com os Estados Unidos. Hydrobiologia foi o principal periódico. O tema de conteúdo dos artigos mais influentes foi relacionado à proliferação de cianobactérias. De acordo com os resultados da análise da estrutura conceitual, o fitoplâncton, a qualidade da água, a eutrofização e as cianobactérias foram os temas mais relevantes. Além disso, os tópicos em alta foram principalmente as alterações climáticas e a degradação. Conclusões Esta análise abrangente permitiu interpretar o desenvolvimento de pesquisas relacionadas ao tema do estudo. lagos rede confiáveis estudados periódicos países relevantes palavraschave palavras chave temático 242 19732023, 19732023 1973 2023, 2023 1973-2023 51 645 5490 484 palavraschave, chave, outros 1027 10 27 10.27% época influente Unidos periódico disso degradação 24 1973202 197 202 1973-202 5 64 549 48 102 1 2 10.27 197320 19 20 1973-20 6 54 4 10.2 19732 1973-2 10. 1973-

Abstract Access to safe drinking water is a fundamental human right and a critical component of sustainable development. This study is conducted in the context of the Taquari-Antas Hydrographic Basin, Rio Grande do Sul, Brazil, and assesses the quality of drinking water in 101 rural properties, focusing on identifying problems of potability, especially regarding excessive fluoride concentration, which is a known problem for the water in this region. The study involves the collection of water samples from rural properties, a survey to understand water use and quality perception by users, and water quality analysis using established analytical methodologies. Results show that 58.4% of the investigated rural properties have potability issues, with excessive fluoride being the most prevalent concern, affecting 39.6% of properties. High fluoride levels have significant health implications, including dental and skeletal fluorosis, with global relevance. To address this issue, activated bone charcoal filtration systems were installed in ten properties, effectively reducing fluoride concentrations to within acceptable limits for human consumption. These defluoridation systems are characterized as sustainable, cost-effective, and scalable, offering a practical solution for regions facing similar challenges. development TaquariAntas Taquari Antas Basin Sul Brazil 10 concentration region users methodologies 584 58 4 58.4 issues concern 396 39 6 39.6 implications fluorosis relevance issue consumption costeffective, costeffective cost effective, effective cost-effective scalable challenges 1 5 58. 3 39.

Resumo O acesso à água potável segura é um direito humano fundamental e um componente crítico do desenvolvimento sustentável. Este estudo foi realizado no contexto da Bacia Hidrográfica Taquari-Antas, no Rio Grande do Sul, Brasil, e avalia a qualidade da água para consumo em 101 propriedades rurais, com foco na identificação de problemas em sua potabilidade, especialmente em relação à concentração excessiva de flúor, que é um problema conhecido na água desta região. O estudo envolve a coleta de amostras de água em propriedades rurais, uma pesquisa para compreender o uso da água e a percepção da qualidade pelos usuários, e análises da qualidade da água utilizando metodologias analíticas estabelecidas. Os resultados mostram que 58,4% das propriedades rurais investigadas apresentam problemas de potabilidade, sendo o excesso de flúor a preocupação mais prevalente, afetando 39,6% das propriedades. Altos níveis de flúor têm implicações significativas para a saúde, incluindo fluorose dentária e esquelética, com relevância global. Para enfrentar esse problema, sistemas de filtração com carvão ativado de osso foram instalados em dez propriedades, reduzindo eficazmente as concentrações de flúor para níveis aceitáveis para consumo humano. Esses sistemas de defluoretação são caracterizados como sustentáveis, economicamente viáveis e escaláveis, oferecendo uma solução prática para regiões que enfrentam desafios semelhantes. sustentável TaquariAntas, TaquariAntas Taquari Antas, Antas Taquari-Antas Sul Brasil 10 potabilidade região usuários estabelecidas 584 58 4 58,4 prevalente 396 39 6 39,6 saúde esquelética global sustentáveis escaláveis semelhantes 1 5 58, 3 39,

This investigation describes the synthesis of eugenol analogs presenting 1,2,3-triazole fragments and evaluation of their antileishmanial activity. The alkylation of guaiacol (1) with allyl bromide afforded 1-(allyloxy)-2-methoxybenzene (2) (93% yield). The Claisen rearrangement conducted with 1 gave ortho eugenol (3) (82% yield). Alkylation procedures performed with 3 produced 1-allyl-3-methoxy-2-(prop-2-yn-1-yloxy)benzene (4) (73% yield) and 1-allyl-3-methoxy-2-(pent-4-yn-1-yloxy)benzene (6) (53% yield). The copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) reactions involving alkynes 4 and 6 with different benzylic azides afforded twenty-two eugenol analogs with 1,2,3-triazole functionalities (48-93% yield). We screened the compounds at 10 μmol L 1 against Leishmania braziliensis intracellular amastigotes during macrophage infection. The action of these compounds was compared with the known leishmanicidal drug amphotericin B. None of the analogs were toxic to macrophages at 10 μmol L-1. The cytotoxic concentration at 50% (CC50), effective concentration at 50% (EC50), and selectivity index (SI) were determined to the best compounds 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-chlorobenzyl)-1 H-1,2,3-triazole (8c) and 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-trifluoromethoxybenzyl)-1 H-1,2,3-triazole (8h). They showed a significant leishmanicidal effect, with EC50 of 28.09 µmol L-1 (8c) and 52.03 µmol L-1 (8h). The SIs were 9.7 for 8c and > 5.7 for 8h. These compounds have the potential as new leishmanicidal agents against L. braziliensis and may represent a starting point for the development of alternative treatments for cutaneous leishmaniasis. 1,2,3triazole 123triazole triazole 1,2,3 2 activity (1 1allyloxy2methoxybenzene allyloxymethoxybenzene allyloxy methoxybenzene (2 93% 93 (93 yield. yield . (3 82% 82 (82 1allyl3methoxy2prop2yn1yloxybenzene allylmethoxypropynyloxybenzene methoxy prop yn yloxy benzene (4 73% 73 (73 1allyl3methoxy2pent4yn1yloxybenzene allylmethoxypentynyloxybenzene pent (6 53% 53 (53 copperIcatalyzed copper I catalyzed alkyneazide alkyne azide CuAAC (CuAAC twentytwo twenty two 4893% 4893 48 (48-93 infection B L1. L1 1. 50 CC50, CC50 CC , (CC50) EC50, EC (EC50) SI (SI 42allyl6methoxyphenoxymethyl14chlorobenzyl1 allylmethoxyphenoxymethylchlorobenzyl phenoxymethyl chlorobenzyl 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-chlorobenzyl)- H1,2,3triazole H123triazole Htriazole H c (8c 42allyl6methoxyphenoxymethyl14trifluoromethoxybenzyl1 allylmethoxyphenoxymethyltrifluoromethoxybenzyl trifluoromethoxybenzyl 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-trifluoromethoxybenzyl)- 8h h (8h) effect EC5 2809 28 09 28.0 L- 5203 52 03 52.0 97 9 7 9. 57 5 5. leishmaniasis 3triazole 123 1,2, ( (9 8 (8 yloxybenzene (7 (5 489 (48-9 CC5 (CC50 (EC50 methoxyphenoxymethyl 42allyl6methoxyphenoxymethyl14chlorobenzyl 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-chlorobenzyl) H1 42allyl6methoxyphenoxymethyl14trifluoromethoxybenzyl 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-trifluoromethoxybenzyl) (8h 280 0 28. 520 52. 12 1,2 (48- (CC5 (EC5 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-chlorobenzyl 4-((2-allyl-6-methoxy)phenoxymethyl)-1-(4-trifluoromethoxybenzyl 1, (48 (CC (EC

Abstract The cold roller die process increases the tensile strength and decreases the ductility of steel wire. Annealing heat treatment is applied to restore mechanical properties, but this is a costly process. This research shows that it is possible to control the mechanical properties of ultra-low carbon steel wire inducing the Bauschinger effect and relieving residual steel stresses. The present study uses several pulleys to promote and control the Bauschinger effect magnitude through alternated cyclic bending. A Completely Randomized Design, a regression study, and the Akaike Information Criterion were used to understand the relationship between the quantity and diameter of pulleys and the influence on the magnitude of the Bauschinger effect. Statistical models showed that it is possible to have a maximum increase of 103% in uniform elongation and a maximum decrease of 14% in yield strength. An interaction between the factors studied in controlling the Bauschinger effect magnitude was confirmed. ultralow ultra low stresses bending Design 103 14 confirmed 10 1

Abstract The control of weeds in agriculture is mainly conducted with the use of synthetic herbicides. However, environmental and human health concerns and increased resistance of weeds to existing herbicides have increased the pressure on researchers to find new active ingredients for weed control which present low toxicity to non-target organisms, are environmentally safe, and can be applied at low concentrations. It is herein described the synthesis of glycerol-fluorinated triazole derivatives and evaluation of their phytotoxic and cytogenotoxic activities. Starting from glycerol, ten fluorinated triazole derivatives were prepared in four steps. The assessment of them on Lactuca sativa revealed that they present effects on phytotoxic and cytogenotoxic parameters with different degrees of efficiency. The compounds 4a, 4b, 4d, 4e, 4i, and 4j have pre-emergent inhibition behavior, while all the investigated compounds showed post emergent effect. Mechanism of action as clastogenic, aneugenic, and epigenetic were observed in the lettuce root meristematic cells, with alterations as stick chromosome, bridge, delay, c-metaphase, and loss. It is believed that glycerol-fluorinated triazole derivatives possess a scaffold that can be explored towards the development of new chemicals for the control of weed species. However nontarget non target organisms safe concentrations glycerolfluorinated glycerol activities steps efficiency 4a 4b b 4d d 4e e 4i i j preemergent pre behavior effect clastogenic aneugenic cells chromosome bridge delay cmetaphase, cmetaphase c metaphase, metaphase c-metaphase loss species

Eugenol (C10H12O2, 4-allyl-2-methoxyphenol) is a phenolic natural product that has several biological activities and possibilities of applications. It is herein described the synthesis of eugenol-fluorinated triazole derivatives and evaluation of their fungicidal activity. The reaction of eugenol with epichlorohydrin resulted in the preparation of (±)-2-((4-allyl-2-methoxyphenoxy)methyl)oxirane (1) in 88% yield. The azidolysis of 1 with sodium azide gave the azido-alcohol (±)-1-(4-allyl-2-methoxyphenoxy)-3-azidopropan-2-ol (2) in 94% yield. The CuAAc reaction between compound 2 and different terminal alkynes afforded a series of eleven derivatives (3a 3k) within 48-80% yield. All the synthesized compounds were characterized by infrared (IR) and nuclear magnetic resonance (1H and 13C) spectroscopies and high-resolution mass spectrometry. The in vitro inhibitory activity of the compounds on the mycelial growth of a strain of Colletotrichum sp., that causes anthracnose disease on papaya fruits, was evaluated. The best result was observed for compound 1-(4-allyl-2-methoxyphenoxy)-3-(4-(2-fluorophenyl)-1H-1,2,3-triazol-1-yl)propan-2-ol (3d) that showed a mean growth-inhibition zone of 5.10 mm in a well-diffusion assay, and may serve as a template for additional structural modifications, aiming for more potent fungicidal activity.

The control of fungal species in agriculture is mainly conducted with the use of contact or systemic fungicides. However, environmental and human health concerns and increased resistance of fungal species to existing fungicides have increased the pressure on researchers to find new active ingredients for fungal control which present low toxicity to non-target organisms, are environmentally safe, and can be applied at very low concentrations. It is herein described the synthesis of eleven glycerol triazole containing compounds (ten of them fluorinated derivatives) and evaluation of their fungicidal activity. Eight out of eleven synthesized compounds are novel and all of the glycerol derivatives were characterized using infrared (IR), nuclear magnetic ressonance (NMR), and mass spectrometry (MS) techniques. Theoretical calculations were also carried out and the results are discussed. Starting from glycerol, the triazole derivatives were prepared in four steps. Evaluation of them against Colletrotricum gloesporioides showed that compound 1-((2,2-dimethyl-1,3 dioxolan-4-yl)methyl)-4-(2-fluorophenyl)-1H-1,2,3-triazole (4d) (ED50 = 59.14 µg mL-1) was slightly more active than commercial fungicide tebuconazole (61.35 µg mL-1). Compound 4d presented attractive physicochemical features for agrochemical purposes as revealed by the calculated physicochemical parameters. It is believed that glycerol-fluorinated triazole derivatives can be explored towards the development of new chemicals for the control of fungal species.

A novel free PhotoMetrix UVC is proposed for both the operation of a universal serial bus video camera (UVC) and multivariate image analysis, allowing a full solution for point-of-use analysis. A UVC was placed in an open-source 3D-printed chamber illuminated by a white light-emitting diode (LED) with controlled intensity of light. The digital images captured were converted into red, green, and blue (RGB) histograms, and regression models were used within the app. As a proof-of-concept, four adulterants in raw milk samples were determined. The coefficient of determination (R2Cal) for all models was higher than 0.99, and no significant differences (p < 0.05) between the measured and predicted values were identified. The root mean square error of calibration (RMSEC) and root mean square error of cross validation (RMSECV) were satisfactory, with values less than 0.1 and 0.7 g L-1, respectively. The recoveries ranged from 90 to 120% in spiked milk samples, and partial least square (PLS) models showed root mean square error of prediction (RMSEP) of 0.28, 0.33, 0.48 and 0.39 g L-1 for chloride, hypochlorite, hydrogen peroxide and starch, respectively. The PhotoMetrix UVC app was feasible for the colorimetric chemical analysis using a smartphone improving the applicability, mobility, and usability.

Resumo Esta análise aplica a revisão sistemática como metodologia para identificar, analisar e interpretar dados sobre o uso de índices de qualidade da água para consumo humano. Os artigos científicos foram pesquisados nas bases de dados “PubMed”, “Scielo”, “Science Direct” e “Web of Science”, usando as palavras-chave “água potável” e “índice de qualidade da água”, com um intervalo personalizado entre 2000 e 2020. Os resultados indicaram 82573 artigos publicados, sendo 16 deles selecionados após um processo de filtragem. Verificou-se a ocorrência de 11 índices de qualidade da água, incluindo 47 parâmetros de qualidade da água utilizados para avaliar a qualidade da água potável, destacando que os parâmetros pH, nitrato, turbidez, cloreto e sulfato foram os mais utilizados, citados em 10 dos 16 artigos selecionados (62,5%). Sugerimos que pesquisas futuras procurem propor indicadores regionalizados de qualidade da água para consumo, a fim de considerar aspectos locais no processo de avaliação e determinar prioridades de intervenção por órgãos de vigilância em saúde.

Abstract This analysis applied systematic review as a methodology for identifying, analyzing and interpreting data on the use of water quality indices for human consumption. Scientific articles were searched in the “PubMed”, “Scielo”, “ScienceDirect” and “Web of Science" databases, using the keywords “drinking water” and “water quality index”, with a custom interval between 2000 and 2020. The results indicated 82,573 published articles, with 16 of them being selected after a filtering process. The occurrence of 11 water quality indices was verified, including 47 water quality parameters used to assess the quality of drinking water, highlighting that the parameters pH, Nitrate, Turbidity, Chloride and Sulfate were the most used, cited in 10 of the 16 articles selected (62,5%). We suggest that future studies seek to propose regionalized water quality indicators for consumption, in order to consider local aspects in the evaluation process and to determine intervention priorities by health surveillance agencies.

Resumo Esta pesquisa teve como objetivo desenvolver um índice de qualidade da água para abastecimento público na Região do Vale do Rio Pardo, RS, Brasil, IQA-VRP, utilizando o banco de dados da 13ª Coordenadoria Regional de Saúde, totalizando 1175 amostras. Um processo de filtragem resultou em 285 amostras com as variáveis Cloro residual livre, turbidez, cor aparente, pH, fluoretos, coliformes totais e Escherichia coli. Para fins de potabilidade, foi utilizado o Índice de Qualidade da Água desenvolvido pela Companhia de Saneamento de Goiás, Brasil. Realizamos uma calibração multivariada dos pesos de importância dos parâmetros incluídos no índice original, resultando no IQA-VRP. Comparando as medianas dos dois índices, verificou-se que houve diferenças significativas (p<0,05), qualificando o IQA-VRP como mais restritivo. Os resultados indicaram que 89,8% das amostras foram classificadas como produtos não conformes pelo IQA-VRP, enquanto 10,2% foram classificadas como produtos conformes, e das 285 amostras analisadas, 72 amostras de cloro, 12 de coliformes totais, 5 de E. coli, 2 de cor, 200 de flúor e 26 de pH, estavam abaixo ou acima dos valores mínimos ou máximos exigidos pela legislação brasileira. O pH e o flúor correspondem às variáveis críticas da região de estudo, pois foram os parâmetros que apresentaram os maiores aumentos nos pesos relativos comparando o índice original com o calibrado. Esses resultados confirmam o uso do IQA-VRP como ferramenta eficiente para avaliar a qualidade da água potável em sistemas lóticos subtropicais e temperados brasileiros.

Abstract This research developed a Water Quality Index (WQI) for public supply in the Vale do Rio Pardo Region, RS, Brazil (WQI-VRP), from the database of the 13th Regional Health Coordination, totaling 1175 samples. A filtration process resulted in 285 samples with the variables free residual chlorine, turbidity, apparent color, pH, fluoride, total coliforms and Escherichia coli. For water consumption purposes, the Water Quality Index developed by the Sanitation Company of Goiás, Brazil, was used. A multivariate calibration of the weights of importance of the parameters included in the original index was performed, resulting in the WQI-VRP. Comparing the medians of the two indexes, it was found that there were significant differences (p<0.05), qualifying the WQI-VRP as more restrictive. The results indicated that 89.8% of the samples were classified as non-compliant samples by WQI-VRP, while 10.2% were classified as compliant samples. Of the 285 samples analyzed, 72 samples of chlorine, 12 of total coliform, 5 of E. coli, 2 of color, 200 of fluoride and 26 of pH, were below or above the minimum or maximum values required by Brazilian legislation. The pH and fluoride correspond to the critical variables of the study region, as they were the parameters that showed the greatest increases in relative weights when comparing the original index with the calibrated one. These results confirm the use of the WQI-VRP as an efficient tool to assess the quality of drinking water in Brazilian subtropical and temperate lotic systems.

It is herein described the synthesis of a series of thirty novel 1,2,3-triazole-1,4 disubstituted compounds inspired on the known SRPKs inhibitor N-(2-(piperidin-1-yl)-5-(trifluoromethyl)phenyl)isonicotinamide (SRPIN340) and biological evaluation of them against human glioblastoma multiforme cell line U87MG. Starting with 1-fluoro-2 nitro-4-(trifluoromethyl)benzene (1), the substances were prepared via a five-step synthetic route. The crucial step corresponded to the copper-catalyzed cycloaddition reaction between trifluoromethyl phenyl azides and different alkynes. In general, the compounds were obtained with good yields and they were characterized utilizing spectroscopic (IR and NMR) and spectrometric (HRMS) techniques. The evaluation of the synthesized compounds at three different treatment time (24 h, 48 h, and 72 h) and concentrations (50, 100, and 150 µmol L-1) revealed that five derivatives were capable of reducing cell viability by 50% after 72 h of treatment at the highest concentration. On the contrary, three derivatives significantly increased cell viability being this effect more pronounced after 48 h of treatment. In this regard, it stands out the compound 2-((1-(2-morpholino-5-(trifluoromethyl)phenyl)-1H-1,2,3-triazol-4-yl)methyl)isoindoline-1,3-dione (7) which increased cell viability in approximately 300% after 48 h of treatment at 100 µmol L-1. The substances that increased cell viaiblity present as common structural features the presence of a saturated nitrogen-containing six-membered ring and carbonylated fragments.