ABSTRACT Objective: The tyrosine-protein kinase inhibitor, genistein, can inhibit cell malignant transformation and has an antitumor effect on various types of cancer. It has been shown that both genistein and KNCK9 can inhibit colon cancer. This research aimed to investigate the suppressive effects of genistein on colon cancer cells and the association between the application of genistein and KCNK9 expression level. Methods: The Cancer Genome Atlas (TCGA) database was used to study the correlation between the KCNK9 expression level and the prognosis of colon cancer patients. HT29 and SW480 colon cancer cell lines were cultured to examine the inhibitory effects of KCNK9 and genistein on colon cancer in vitro, and a mouse model of colon cancer with liver metastasis was established to verify the inhibitory effect of genistein in vivo. Results: KCNK9 was overexpressed in colon cancer cells and was associated with a shorter Overall Survival (OS), a shorter Disease-Specific Survival (DFS), and a shorter Progression-Free Interval (PFI) of colon cancer patients. In vitro experiments showed that downregulation of KCNK9 or genistein application could suppress cell proliferation, migration, and invasion abilities, induce cell cycle quiescence, promote cell apoptosis, and reduce epithelialmesenchymal transition of the colon cancer cell line. In vivo experiments revealed that silencing of KCNK9 or application of genistein could inhibit hepatic metastasis from colon cancer. Additionally, genistein could inhibit KCNK9 expression, thereby attenuating Wnt/β-catenin signaling pathway. Conclusion: Genistein inhibited the occurrence and progression of colon cancer through Wnt/β-catenin signaling pathway that could be mediated by KCNK9. Objective tyrosineprotein tyrosine protein inhibitor KNCK KCNK Methods TCGA (TCGA patients HT HT2 SW SW48 Results OS, OS , (OS) DiseaseSpecific Disease Specific DFS, DFS (DFS) ProgressionFree Progression Free PFI (PFI proliferation migration abilities quiescence apoptosis line Additionally Wnt/βcatenin Wntβcatenin Wnt/β catenin Wnt β Conclusion SW4 (OS (DFS βcatenin Wntβ

A compact CPW-fed ultra-wideband antenna with dual band-notch characteristic is presented. Two notched frequency bands are obtained by embedding two U-shaped slots in the radiation patch and a rectangle slot in the ground plane. The two notched bands can be controlled by adjusting the length of the responding slots. The proposed antenna is successfully simulated, fabricated and tested. Experimental and numerical antenna shows that the proposed antenna with compact size of 21×28mm2, has an impedance bandwidth range from 3.1GHz to more than 11.0GHz for voltage standing-wave ratio less than 2, expect two notch band frequency 5GHz-6GHz for WLAN and 7.7GHz-8.5GHz for X-band for satellite communications in China.