ABSTRACT: This study evaluated the stability of anaerobic digestion and biogas production from the manure of laying hens fed diets containing different mineral sources and rosemary oil concentrations. Manure was obtained from 288 semi-heavy laying hens, 30 weeks old, and collected for 112 days. A completely randomized design was used in a 2×3 factorial scheme with repeated measures, considering two mineral sources (inorganic and organic) and three concentrations of rosemary oil (0, 100, and 200 mg kg-1). Twelve continuous bench digesters (7.5 kg) were operated with a hydraulic retention time of 30 days and daily loads of 5% total solids, for 90 days. A weekly analysis of influents and effluents was performed by measuring the values of total solids (TS), volatile solids (VS), pH, ammonia nitrogen (ammonia N), intermediary alkalinity (IA), partial alkalinity (PA), and the relationship IA:PA; biweekly analysis of total nitrogen (N) and total phosphorus (P) concentrations; and daily measurement of biogas production data to calculate the biogas production potential of the manure. Manure from laying hens fed with organic minerals has greater potential for biogas production and greater TS and VS reduction than manure from hens supplemented with inorganic minerals. Laying hen diets containing up to 200 mg kg-1 of rosemary oil do not present the risk of anaerobic digestion failure. ABSTRACT 28 semiheavy semi heavy 3 old 11 23 2 2× measures 0, 0 (0 100 20 kg1. kg1 kg 1 . kg-1) 7.5 75 7 5 (7. 9 TS, , (TS) VS, (VS) pH N, N N) IA, IA (IA) PA, PA (PA) IAPA IA:PA (N P (P kg- failure ( 10 7. (7 (TS (VS (IA (PA

RESUMO: O objetivo deste estudo foi avaliar a estabilidade no processo de digestão anaeróbia e a produção de biogás dos dejetos de poedeiras alimentadas com dietas contendo diferentes fontes de minerais e níveis de óleo de alecrim. Os dejetos foram provenientes de 288 poedeiras semipesadas, com 30 semanas de idade, colhidos por 112 dias. Utilizou-se o delineamento inteiramente casualizado com esquema fatorial 2x3, com medidas repetidas no tempo, sendo as dietas com duas fontes minerais (inorgânica e orgânica) e três níveis de óleo de alecrim (0, 100 e 200 mg kg-1). Foram utilizados 12 biodigestores contínuos (7,5 kg), operados com tempo de retenção hidráulica de 30 dias, com cargas diárias com 5% de sólidos totais, por 90 dias. Foram realizadas análises semanais do afluente e efluente mensurando-se os valores de sólidos totais (ST), sólidos voláteis (SV), pH, nitrogênio amoniacal (N amoniacal), alcalinidade parcial (AP), alcalinidade intermediária (AI) e a relação AP:AI; quinzenalmente análises das concentrações de nitrogênio total (N) e de fósforo total (P) e diariamente mensuradas as produções de biogás para calcular o potencial de produção de biogás do dejeto. Dejetos de poedeiras alimentadas com minerais orgânicos apresentam maior potencial de produção de biogás, maiores reduções de ST e SV em relação àquelas alimentadas com minerais inorgânicos. A adição de óleo de alecrim na dieta de poedeiras não oferece riscos de falência para o processo de digestão anaeróbia. RESUMO 28 semipesadas 3 idade 11 dias Utilizouse Utilizou se 2x3 x inorgânica orgânica 0, 0 (0 10 20 kg1. kg1 kg 1 . kg-1) 7,5 75 7 5 (7, kg, , kg) 9 mensurandose mensurando ST, (ST) SV, (SV) pH N amoniacal, amoniacal) AP, AP (AP) AI (AI APAI AP:AI P (P dejeto inorgânicos 2 2x ( kg-1 7, (7 (ST (SV (AP kg-

Abstract This study aimed to evaluate the effects of mineral sources and rosemary oil levels in the diet of commercial layers on the qualitative and quantitative characteristics of the waste generated. Manure was obtained from 288 commercial laying hens of the HyLine Brown lineage (semi-heavy layers) at 30 weeks of age over a period of 112 d (four cycles of 28 d). A completely randomized design was used with a 2×3 factorial scheme with repeated measures over time (16 repetitions), with two mineral sources (inorganic and organic) and three levels of rosemary oil (0, 100, and 200 mg kg-1). The waste was collected weekly, weighed, and the calculations of waste production, residue coefficient (RC) were performed. Also analyses of total solids (TS), volatile solids (VS), pH, macro-, and micro-minerals were performed. Mineral sources affect the quality characteristics of the manure. The amount of rosemary oil affected the quantitative and qualitative characteristics of the waste generated. It was concluded that diets containing organic minerals resulted in waste with less polluting power, as it had a lower TS, pH, total N, and total P values. Rosemary oil increased manure production in natural matter (NM) and increased the TS and VS values in the manure layer, causing a greater environmental impact. generated semiheavy semi heavy 3 11 four 2 d. . d) 23 2× 16 (1 repetitions, repetitions , repetitions) inorganic 0, 0 (0 100 20 kg1. kg1 kg 1 kg-1) weekly weighed RC (RC performed (TS) VS, (VS) pH macro, macro macro- microminerals micro power N NM (NM layer impact ( 10 kg-1 (TS (VS kg-

Resumo Realizou-se este estudo com o objetivo de avaliar fontes de minerais e de níveis do óleo de alecrim na dieta de poedeiras comerciais sobre as características qualitativas e quantitativas dos dejetos gerados. Os dejetos foram provenientes de 288 poedeiras comerciais da linhagem HyLine Brown (poedeiras semipesadas) com 30 semanas de idade, durante o período de 112 dias (4 ciclos de 28 dias). Foi utilizado delineamento inteiramente casualizado com esquema fatorial 2x3 com medidas repetidas no tempo (16 repetições), sendo duas fontes minerais (inorgânica e orgânica) e três níveis de óleo de alecrim (0, 100 e 200 mg kg-1). Semanalmente os dejetos foram coletados, pesados e realizados os cálculos de produção de dejetos, coeficiente de resíduo (Cr) e as análises de sólidos totais (ST), sólidos voláteis (SV), pH, macro e microminerais. As fontes de minerais afetaram as características qualitativas dos dejetos. Os níveis de óleo de alecrim afetaram as características quantitativas e qualitativas do dejeto gerado. Concluiu-se que dietas contendo mineral orgânico resultam em dejetos com menor poder poluente por apresentar menores valores de ST, pH, N total e P total. O óleo de alecrim aumenta a produção de dejetos na MN e aumenta os valores de ST e SV nos dejetos das poedeiras causando maior impacto ambiental. Realizouse Realizou se gerados semipesadas 3 idade 11 4 ( 2 dias. . dias) x 2x 16 (1 repetições, repetições , repetições) inorgânica orgânica 0, 0 (0 10 20 kg1. kg1 kg 1 kg-1) coletados Cr (Cr (ST) SV, (SV) pH microminerais gerado Concluiuse Concluiu ambiental kg-1 (ST (SV kg-

RESUMO: Realizou-se este estudo com o objetivo de avaliar planos sequenciais de energia metabolizável (EM), mantendo a relação lisina: caloria, para leitões dos 7 ao 30 kg. Foram utilizados 48 leitões, fêmeas, com peso inicial de 6,9 ± 1,2 kg e final de 30,5 ± 3,8 kg, distribuídos em delineamento de blocos ao acaso em três planos nutricionais, sendo: 1- 3.300-3.250-3.200-3.150 Kcal de EM kg-1 de ração; 2- 3.400-3.350-3.300-3.250 Kcal de EM kg-1 de ração; e 3- 3.500-3.450-3.400-3.350 Kcal de EM kg-1 de ração, dos 7 aos 10 kg, dos 10 aos 15 kg, dos 15 aos 20 kg e dos 20 aos 30 kg, respectivamente, com oito repetições e dois animais por unidade experimental. Dos 7 aos 10 kg, não houve efeito (P>0,05) dos planos nutricionais nas variáveis avaliadas. Observou-se, dos 7 aos 15 kg, maiores (P<0,05) consumos de energia e ganho de peso e melhor (P < 0,05) conversão alimentar nos animais alimentados com os planos nutricionais 2 e 3. Os leitões alimentados com os planos nutricionais 2 e 3 apresentaram maior (P<0,05) peso final, consumos de ração, de energia, de lisina digestível, ganho de peso e melhor conversão alimentar nos períodos dos 7 aos 20 kg, dos 7 aos 25 kg e dos 7 aos 30 kg. Recomenda-se o plano sequencial contendo 3.400-3.350-3.300-3.250 Kcal de EM kg-1 de ração dos 7 aos 10 kg, dos 10 aos 15 kg, 15 aos 20 kg e dos 20 aos 30 kg, respectivamente, equivalentes aos níveis de 2.546-2.513-2.501-2.475 Kcal de energia líquida kg-1 de ração.

ABSTRACT: The objective of this study was to evaluate sequential metabolizable energy (ME) plans, maintaining the lysine: calorie ratio for piglets from 7 to 30 kg. Forty eight female piglets with initial weight of 6.9 ± 1.2 kg and final weight of 30.5 ± 3.8 kg were randomly allocated in a randomized complete blocks design to three nutritional plans with eight replicates and two animals per experimental unit. Sequential plans provided four ME levels: plan 1: 3,300-3,250-3,200-3,150 kcal ME kg-1, plan 2: 3,400-3,350-3,300-3,250 kcal ME kg-1, and plan 3: 3,500-3,450-3,400-3,350 kcal ME kg-1 of feed, from 7 to 10 kg, 10 to 15 kg, 15 to 20 kg and 20 to 30 kg, respectively. From 7 to 10 kg, there was no effect (P>0.05) of the nutritional plans in the evaluated variables. From 7 to 15 kg, a higher (P<0.05) energy intake and weight gain and a better (P < 0.05) feed conversion was observed in animals fed with nutritional plans 2 and 3. Nutritional plans 2 and 3 resulted in higher (P<0.05) final weight, feed intake, energy intake, digestible lysine intake, weight gain, and better feed conversion in the 7 to 20 kg, 7 to 25 kg and 7 to 30 kg. It is recommended the sequential plan containing 3,400-3,350-3,300-3,250 kcal ME kg-1 of feed from 7 to 10 kg, from 10 to 15 kg, 15 to 20 kg, and from 20 to 30 kg; respectively, equivalent to levels of 2,546-2,513-2,501-2,475 kcal net energy (NE) kg-1 of feed for piglets.

ABSTRACT The objective of this study was to evaluate sequential digestible phosphorus levels for gilts from 30 to 100 kg. Sixty gilts were used, with an initial weight of 29.98±3.37 kg and final weight of 100.66±8.82 kg, distributed in a randomized block design, with five nutritional digestible phosphorus levels: 2.19-1.88-1.68, 2.69-2.38-2.18, 3.19-2.88-2.68, 3.69-3.38-3.18, and 4.19-3.88-3.68 g kg−1, respectively, for the growth phases 30 to 50 kg, 50 to 70 kg, and 70 to 100 kg, with six replicates and two animals per pen. The nutritional plans did not influence the final weight, days to reach 100 kg, total and daily weight gain, total and daily feed intake, crude protein intake, digestible lysine intake, or metabolizable energy intake. An increase in digestible phosphorus and calcium intake was observed according to the increase in the digestible phosphorus level in the diet. There was a significant effect on feed conversion. No effect of nutritional plans was observed for backfat thickness, muscle depth, loin eye area, lean meat percentage, or carcass bonus index. There was no difference in the production of dry and natural matter or the residue coefficient. The effect of the nutritional digestible phosphorus levels in the contents of total solids and volatile solids was verified. There was a difference in the concentration of total nitrogen and total phosphorus in the manure. The nutritional plan with 3.19-2.88-2.68 and 3.69-3.38-3.18 g kg−1 of digestible phosphorus results in better feed conversion than the basal diet. For a lower excretion of phosphorus in the manure, the recommended nutritional plan is 2.19-1.88-1.68 g kg−1 of digestible phosphorus.

RESUMO: Esta pesquisa foi realizada com o objetivo de investigar a utilização de extrato etanóico de própolis como alternativa aos antibióticos melhoradores de desempenho para leitões. Foram avaliados 70 leitões desmamados aos 21 dias de idade, com pesos iniciais de 7,3±0,4kg e final de 24,9±1,2kg. Estes foram distribuídos em delineamento de blocos ao acaso, com cinco dietas: controle positivo - dieta com 200ppm de antibiótico; controle negativo - dieta sem promotor de crescimento; dietas com 100, 500 e 1.000ppm de extrato etanóico de própolis marrom, com sete repetições de dois leitões cada. A duração do período experimental foi de 35 dias. As dietas não influenciaram (P>0,05) no consumo de ração diária, ganho de peso diário, peso final e a conversão alimentar dos animais. O perfil bacteriológico da swab nasal apresentou predomínio de bactérias Gram positivas comumente associadas às membranas das mucosas em todas as coletas realizadas. As swabs retais não apresentaram isolamento atípico. A utilização do extrato etanóico de própolis nas dietas não altera o desempenho de leitões desmamados, bem como não afeta o perfil bacteriológico, escore fecal e a ocorrência de diarreia dos leitões.

ABSTRACT: This study was conducted to assess the use of propolis ethanolic extract as an alternative to performance-enhancing antibiotics for piglets. Seventy piglets weaned at 21 days of age, with initial weights of 7.3±0.4kg and final weights of 24.9±1.2kg, were randomly assigned to five diets: positive control (diet with 200ppm antibiotic); negative control (diet without growth promoter); and diets with 100, 500, and 1,000ppm of brown propolis ethanolic extract, with seven replicates of two piglets each. The duration of the experimental period was 35 days. The diets did not influence (P>0.05) daily feed intake, daily weight gain, final weight, and feed conversion of animals. The bacteriological profile of the nasal swab presented a predominance of gram-positive bacteria commonly associated with the mucous membranes in all samples. Rectal swabs did not present atypical bacterial isolates. The use of ethanolic propolis extract in diets did not alter the performance of weaned piglets, nor did it affect the bacteriological profile, fecal score, or the occurrence of diarrhea in piglets.

ABSTRACT This study was carried out to evaluate the levels of digestible phosphorus in diets for barrows with a high potential for lean meat deposition from 50 to 80 kg. Eighty barrows, with an initial weight of 47.93±3.43 kg, were distributed in completely randomized blocks, with each group given five levels of digestible phosphorus (1.86, 2.23, 2.61, 2.99, and 3.36 g kg−1). There were eight replicates, and two animals per experimental unit. Phosphorus levels did not significantly influence feed intake, weight gain, or feed conversion ratio. Daily digestible phosphorus intake increased linearly as levels of phosphorus in the diet were increased. Phosphorus levels did not significantly influence muscle depth, loin eye area, backfat thickness, or the percentage and quantity of lean meat in the carcass. A linear increase was observed for feeding cost as the levels of digestible phosphorus in the diet were increased, and the level of 1.86 g kg−1 cost 29.4% less when compared with the level of 2.61 g kg−1. The dry matter, natural matter, the coefficient of the residue, and volatile solids of the waste were not significantly influenced by phosphorus levels. Conversely, it was possible to observe an increasing linear effect for total solids, total phosphorus, and total nitrogen in the waste of animals receiving diets with increased levels of digestible phosphorus. The level of 1.86 g kg−1, which corresponded to a daily intake of 4.77 g−1 of digestible phosphorus, meets the requirements of barrows weighing 50 to 80 kg.