Amebiasis is an infection caused by Entamoeba histolytica. However, differentiation between E. histolytica and Entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. The purpose of the present study was to evaluate a Multiplex -PCR for detection and differentiation of E. histolytica from E. dispar from fresh stool samples in comparison with the coproantigen commercial ELISA. Microscopic examination of stools using the Coprotest method, detection of stool antigen by enzyme-linked immunosorbent assay kit and a home made Multiplex-PCR, were used for the diagnosis of amoebiasis infection. Analysis of the 127 stools samples by microscopy examination demonstrated that only 27 (21%) samples were positive for E. histolytica/E. dispar complex. Among these stool samples, 11 were positive by Multiplex-PCR, with nine presenting the diagnostic fragment characteristic of E. dispar (96 bp) and two presenting diagnostic fragment of E. histolytica (132 bp). Among negative samples detected by microscopic examination, three positive samples for E. dispar and one positive for E. histolytica by Multiplex-PCR was observed. This denotes a low sensibility of microscopic examination when a single stool sample is analyzed. Assay for detection of E. histolytica antigen was concordant with multiplex-PCR in relation to E. histolytica. Statistical analysis comparing the sensibility tests was not done because of the low number of E. histolytica cases. The results demonstrate the importance of the specific techniques use for the differentiation between E. histolytica and E. dispar.

São descritos dois casos de fasciolíase em áreas rurais do Rio de Janeiro, endêmicas para esquistossomose, sendo ambos surpreendidos durante inquéritos coprológicos. O paciente de Paracambi queixava-se de tonteira. A paciente de Sumidouro queixava-se de tonteira, cansaço e tosse, tendo sido tratada com praziquantel; seus exames de controle foram negativos.

We describe two cases of fascioliasis from rural areas of Rio de Janeiro that are endemic for schistosomiasis, both of which were found during a coprological survey. The patient from Paracambi complained of dizziness. The patient from Sumidouro complained of dizziness, tiredness and cough. She was treated with praziquantel and her stool parasitological examination became negative.

Este texto apresenta o caminho pelo qual temos evoluído em direção a uma solução para os problemas de Guapirimirim (Rio de Janeiro, Brasil) na área de saúde e ambiente. Nosso objetivo vem sendo atingido a partir de um estudo interdisciplinar e de uma parceria que vem assimilando, gradativamente, conceitos e práticas propostos por teóricos contemporâneos para lidar com complexidade, incerteza e avaliação. Os conceitos de ciência "pós-normal", "comunidade ampliada de pares" e "transdisciplinaridade" servem de base para o entendimento do empreendimento científico em curso naquele município, e para a formulação de uma proposta para sua continuidade.

This article reports the way in which we are evolving towards a solution for health and environmental problems in Guapimirim (Rio de Janeiro, Brasil). Our goal is being achieved trough an interdisciplinary study and through a partnership, which is gradually getting concepts and praxis proposed by contemporary theorists to deal with complexity, uncertainty and evaluation. The concepts of "post-normal science", "extended peer community" and "transdisciplinarity" were basic to our understanding of the scientific experience in course at the Municipality and to the proposals for its continuity.

Este texto apresenta o caminho pelo qual temos evoluído em direção a uma solução para os problemas de Guapirimirim (Rio de Janeiro, Brasil) na área de saúde e ambiente. Nosso objetivo vem sendo atingido a partir de um estudo interdisciplinar e de uma parceria que vem assimilando, gradativamente, conceitos e práticas propostos por teóricos contemporâneos para lidar com complexidade, incerteza e avaliação. Os conceitos de ciência "pós-normal", "comunidade ampliada de pares" e "transdisciplinaridade" servem de base para o entendimento do empreendimento científico em curso naquele município, e para a formulação de uma proposta para sua continuidade.

This article reports the way in which we are evolving towards a solution for health and environmental problems in Guapimirim (Rio de Janeiro, Brasil). Our goal is being achieved trough an interdisciplinary study and through a partnership, which is gradually getting concepts and praxis proposed by contemporary theorists to deal with complexity, uncertainty and evaluation. The concepts of "post-normal science", "extended peer community" and "transdisciplinarity" were basic to our understanding of the scientific experience in course at the Municipality and to the proposals for its continuity.