Abstract Many biological activities are described for the Clusiaceae family. Clusia fluminensis, a species from Brazilian flora, is mainly employed for ornamental purposes. This review aimed to depict the current knowledge of C. fluminensis from a bioprospecting standpoint. “Clusia fluminensis” search term was applied in Scopus, Web of Science, PubMed and Bireme databases according to PRISMA-ScR statement. Selected papers on Phytochemistry or Bioactivity followed hand searching procedures. Bioactivity preclinical studies considered in vitro or in vivo biological systems, treated with plant extracts or isolated compounds. The outcomes were compared with standard or no treatment control groups. Critical appraisal of individual trials considered completeness in the research fields. Our results showed that 81% of the selected papers presented high level of completeness, 69% revealed phytochemical parameters and 31% biological applications of plant extracts and isolated compounds. Polyisoprenylated benzophenones, terpenoids, sterols and phenolic compounds were identified. Antiviral, insecticidal and snake antivenom activities were reported. In conclusion, the phytochemical data reinforce the reported activities. Potential applications in personal care, nutritional supplementation and pharmaceutical, food, chemical or textile industries were also identified. Toxicological and phytochemical complementary studies may be required. family flora purposes C standpoint Scopus Science PRISMAScR PRISMA ScR statement procedures systems groups fields 81 69 31 benzophenones terpenoids identified Antiviral conclusion care pharmaceutical food required 8 6 3

ABSTRACT Plants represent a huge source of substances, with pharmacological potential. Brazil has a diversity of agricultural insect pests and an urgent need for safer methods of insect control. Dysdercus peruvianus (Guerin-Meneville, 1831), Pyrrhocoridae, is an economically important species of the Order Hemiptera and a pest of cotton (Gossypium hirsutum L., Malvaceae). Secondary metabolites in stems of Clusia hilariana Schltdl., Clusiaceae, such as terpenes and benzophenones, have been reported to be insecticidal. The present study investigated the effects of semi-purified fractions of hexane crude extracts from male C. hilariana stems on development of D. peruvianus. Biological parameters at different stages of development including body malformations, range of molting period and toxicity were evaluated. Most insects died and failed to develop due to attachment of their exuviae to the abdomen. Deformations of wings and defective tarsi also occurred. The secondary metabolites from semi-purified fractions of C. hilariana caused mortality, interference in molting and metamorphosis, and body deformations, probably by interacting with the neuroendocrine system. The results demonstrate the potential of C. hilariana extracts as an alternative for the control of the phytophagous insect D. peruvianus and for the development of environmentally safe and biodegradable bio insecticides.

ABSTRACT The effects of the hexanic extracts of the fruits and flowers of Clusia fluminensis Planch. & Triana, Clusiaceae, as well as their main constituents, the triterpene lanosterol and the benzophenone clusianone, were evaluated on hemipterans Dysdercus peruvianus and Oncopeltus fasciatus. The topical treatments of insects with the hexanic extracts significantly affected the survival of O. fasciatus, but not that of D. peruvianus. Concomitantly, extracts delayed the development of both hemipterans. Moreover, isolated lanosterol significantly reduced both the survival and development of O. fasciatus and D. peruvianus, while clusianone only reduce the survival of D. peruvianus and marginally inhibited the development of both insects. The results show the specific activity of lanosterol and clusianone against the two evaluated insect species and indicate the potential of compounds derived from C. fluminensis for the development of specific biopesticides for the control of agricultural pests. Subsequent work will examine the mode of action of lanosterol and clusianone isolates from C. fluminensis.

Studies evaluated the effects of hexanic extracts from the fruits and flowers of Clusia fluminensis and the main component of the flower extract, a purified benzophenone (clusianone), against Aedes aegypti. The treatment of larvae with the crude fruit or flower extracts from C. fluminensis did not affect the survival of Ae. aegypti (50 mg/L), however, the flower extracts significantly delayed development of Ae. aegypti. In contrast, the clusianone (50 mg/L) isolate from the flower extract, representing 54.85% of this sample composition, showed a highly significant inhibition of survival, killing 93.3% of the larvae and completely blocking development of Ae. aegypti. The results showed, for the first time, high activity of clusianone against Ae. aegypti that both killed and inhibited mosquito development. Therefore, clusianone has potential for development as a biopesticide for controlling insect vectors of tropical diseases. Future work will elucidate the mode of action of clusianone isolated from C. fluminensis.