ABSTRACT Two experiments were conducted to evaluate the digestibility and energy of distillers dried grains with solubles (DDGS) from corn and sorghum with or without xylanase enzyme in diets for pigs. The values of dry matter (DM), organic matter (OM), crude protein (CP), mineral matter (MM), ether extract (EE), neutral detergent fiber (NDF), gross (GE), digestible (DE), metabolizable energy (ME), and digestibility coefficients (DC) were determined. In the experiment 1, we used eight barrows of 26.15±3.45 kg with repeated measures in three periods of five days of collection. The treatments consisted of a reference diet (RD); RD with 200 g kg−1 corn DDGS or sorghum DDGS; and RD with 400 g kg−1 corn DDGS or sorghum DDGS. Corn DDGS showed higher values of DE, ME, and DC of EE, GE, and CP. Inclusions of 400 g kg−1 of the test ingredients resulted in higher values of DE and ME and trend for higher DC of DM, OM, and CP. In experiment 2, nine barrows weighing 34.91±1.46 kg were fed RD, 200 g kg−1 corn DDGS or 200 g kg−1 corn DDGS plus xylanase enzyme. The inclusion of xylanase did not influence the digestibility and energy values in diets containing corn DDGS. Values of DE and ME of corn DDGS were 3,477 and 3,277 kcal kg−1, respectively, for the substitution of 200 g kg−1 and 3,761 and 3,068 kcal kg−1 for the substitution of 400 g kg−1 RD. For sorghum DDGS, DE and ME values were 3,030 and 2,863 kcal kg−1, respectively, for replacement of 200 g kg−1 and 3,398 and 3,296 kcal kg−1 for substitution of 400 g kg−1 RD. Levels of up to 400 g kg−1 do not influence the DE and ME of the diets but impair the digestibility coefficient of DM, OM, CP, EE, MM, and NDF. The use of xylanase enzyme in diets containing 200 g kg−1 of corn DDGS does not affect the digestibility of the diets.

Resumo: O objetivo deste trabalho foi avaliar os efeitos da suplementação dietética de glutamina e ácido glutâmico no turnover do carbono (δ13C) em músculos de leitões, no desempenho dos animais e na viabilidade econômica das rações. As dietas consistiram em: controle (C), 1% de glutamina (G) e 1% de ácido glutâmico (AG). No experimento I, 111 leitões foram usados para avaliar o turnover do carbono nos músculos Masseter, Psoas major e Abdominal internal oblique, quanto à composição isotópica e à substituição do 13C no tempo. No experimento II, 72 leitões foram alocados em blocos ao acaso (oito repetições por tratamento e três animais por unidade experimental), para avaliar o desempenho dos animais e a viabilidade econômica das rações. A dieta AG promoveu a melhor aceleração de 13C nos músculos estudados. Não se constatou efeito das dietas nas variáveis de desempenho. As dietas G e AG apresentaram maiores custos que a dieta C. Embora a suplementação com 1% de glutamina ou ácido glutâmico acelere o turnover do carbono nos músculos estudados, o que sugere rápida recuperação no pós-desmame e comprova o efeito anabólico desses aditivos, seu uso não é economicamente viável para leitões recém-desmamados.

Abstract: The objective of this work was to evaluate the effects of dietary glutamine and glutamic acid supplementation on carbon turnover (δ13C) in the muscles of piglets, on animal performance, and on feed economic feasibility. The diets consisted of: control (C), 1% glutamine (G), and 1% glutamic acid (GA). In experiment I, 111 piglets were used to assess carbon turnover in the Masseter, Psoas major, and Abdominal internal oblique muscles in terms of their isotopic composition and 13C substitution over time. In experiment II, 72 piglets were assigned randomly to blocks (eight replicates per treatment and three animals per experimental unit), in order to evaluate animal performance and feed economic feasibility. The GA diet promoted the best 13C acceleration in the studied muscles. No diet effects were observed on performance variables. The G and GA diets presented higher costs than the C diet. Although supplementation with 1% glutamine or glutamic acid accelerates carbon turnover in the studied muscles, which suggests a faster recovery during post-weaning and proves the anabolic effect of these additives, its use is not economically viable for weanling piglets.

ABSTRACT The objective of this study was to determine the digestibility coefficient (DC) of nutrients and the digestible energy (DE), and metabolizable energy (ME) values of distillers dried grains with solubles (DDGS) produced in Brazil by different methods. Eight barrows with 23.3±4.1 kg were housed in metabolic cages in a randomized block design and fed diets containing 0, 200, 400, and 600 g kg–1 of corn DDGS. We determined the digestibility of dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), mineral matter (MM), neutral detergent fiber (NDF), and gross energy (GE) by the total collection (TC) and chromium oxide marker (Cr) methods. Distillers dried grains with solubles provided the respective DM, OM, CP, EE, MM, NDF, and GE values of 910, 863, 286, 66.9, 46.8, 500 g kg–1, and 4,780 kcal kg–1. The DE and ME values of DDGS were 3,739 and 3,401 kcal kg–1 and 3,691 and 3,213 kcal kg–1 as determined by TC and Cr methods, respectively. The growing inclusion of DDGS did not affect the DE or ME values. The digestibility coefficients of DM, OM, CP, EE, MM, and NDF were 767, 765, 827, 691, 883, and 821 g kg–1, respectively, by the total collection method. The Cr underestimated the values of all variables compared with TC method. Levels up to 600 g kg–1 of the test ingredient do not influence the DE and ME of DDGS, but compromises the digestibility coefficients of DM, OM, and NDF.

ABSTRACT This research aimed to evaluate enzyme supplementation in diets with different nutritional levels for pigs in their feces excretion and anaerobic digestion. Fifty four gilts were placed on diets formulated to achieve 100 or 95 or 90 % of a pig's nutrient requirements, with the addition of an enzyme complex containing amylase, β-glucanase, cellulase, pectinase, xylanase, protease and phytase (diets 100E, 95E and 90E) or without the enzymes (diets 100, 95 and 90). The trial was divided into three periods (1, 1-2 and 1-3) based on the animal weight. The enzyme complex reduced (p < 0.05) excretions per period and per unit of weight gain (residue coefficients) of organic matter (OM) in Period 1, of N, Ca, Zn and Na in Period 1-2, and of Na excretions and residue coefficients in Period 1-3. Animals fed diet 95E had lower excretions (p < 0.05) of dry (DM), organic and mineral matters, N, Ca, Fe, Mn and Cu, and lower residue coefficients (p < 0.05) of DM, OM, P, Fe, Mn and Cu compared with the animals fed diet 95, in Period 1-3. Numerically, the enzyme complex improved biogas and methane production potentials in anaerobic digestion of the feces. The use of an enzyme complex in a diet formulated to provide 95 % of the gilts' nutrient requirements reduced fecal excretion and positively affected the anaerobic digestion of feces. These data show that pig nutrition and feeding are important tools for reducing the potential environmental impact of pig production.

ABSTRACT The objective of this study was to evaluate performance and carcass and meat characteristics of finishing pigs fed diets with different levels of glycerin, while maintaining the electrolyte balance. Forty barrows with an initial weight of 97.76±13.44 kg were distributed, in a randomized block design, into four treatments with five replicates. Treatments consisted of diets containing 0, 5, 10, and 15% glycerin. Inclusions of salt and sodium bicarbonate were adjusted to balance the sodium content and maintain the same electrolyte balance of the diets as a function of glycerin inclusions. In vivo backfat thickness, daily feed intake, daily weight gain, and feed conversion were measured. After the animals were slaughtered, pH and temperature were measured 45 min and 24 h postmortem, and backfat thickness, loin-eye area, drip loss, and colorimetry were determined. Glycerin inclusion levels did not influence the performance of barrows. Hot carcass weight, dressing percentage, and lightness had higher values when pigs were fed diets containing 2.32, 2.43, and 2.61% glycerin, respectively. The other carcass and meat characteristics were not influenced. Glycerin can be used in finishing pig diets without compromising results of performance or carcass and meat characteristics up to the inclusion level of 15%.

Dietary fiber is an important component, which has a direct effect on intake, digestion, and absorption of nutrients; and also alters intestinal microbiota and morphology according to solubility. One digestibility trial and one performance experiment were performed to evaluate the effects of sources of fiber in diets for 21 day weaned piglets. The experimental diets used in both trials consisted of a control diet and diets with purified cellulose, soybean hulls or citrus pulp as a main source of dietary fiber. To evaluate the digestibility of nutrients (Assay 1), the total feces and urine collection method was used. The fiber sources did not affect nutrient digestibility, except for soluble fiber, which increased with the inclusion of citrus (Citrus sinensisL.) pulp. To evaluate performance, morphophysiology and microbiology of the digestive tract of weaned piglets, a total of 32 castrated male piglets was used. Slaughter of animals was implemented at 35 and 50 days of age. The use of soybean (Glycine max L.) hulls and citrus pulp in diets increased the number of goblet cells and the density of villi in the jejunum. The viscosities of stomach and cecum contents increased due to the addition of citrus pulp. Soybean hulls and the citrus pulp included in diets reduced the occurrence of E. coli in the small intestines of piglets slaughtered at 35 days of age. Among the fiber sources, purified cellulose in piglet diets promotes better performance of animals, due to the modulation of the small intestine microbiota, with lower E. coli occurrence resulting in higher villus density.

Objetivou-se avaliar os efeitos de níveis de polpa cítrica (PC), com ou sem adição de um complexo enzimático nas rações, sobre o desempenho dos suínos e as características de carcaça. Utilizou-se 72 suínos, com 25,71 ± 7,51 e 98,81 ± 10,64kg de pesos inicial e final, respectivamente. As dietas foram formuladas para as fases: 1 - 70 aos 101; 2 - 102 aos 130; 3 - 131 aos 143 dias de idade. Como não houve redistribuição dos animais nos blocos no início das fases 2 e 3, os dados foram avaliados nos períodos: 1 - 70 aos 101; 2 - 70 aos 130 e 3 - 70 aos 143 dias de idade. A inclusão de até 15% de PC, com ou sem a adição do complexo enzimático nas dietas, diminuiu o consumo diário de ração nos Períodos 1 e 2, e melhorou a conversão alimentar no Período 2, porém, os animais apresentaram respostas adaptativas no Período 3. O rendimento de carcaça e a relação gordura/carne diminuíram linearmente, na medida em que aumentaram os níveis de PC, na ausência do complexo enzimático. Na presença do produto, a relação gordura/carne apresentou comportamento quadrático, e o nível de 7,78% de PC determinou a menor relação. A inclusão de até 15% de PC com ou sem a adição do complexo enzimático nas dietas para suínos, não proporciona diferenças no desempenho e pode ser uma alternativa alimentar, sem afetar as características de carcaça.

The purpose is to evaluate the effects of levels of citrus pulp (CP), over the aperformance and the carcass characteristics, with or without the addition of an enzyme complex in the feed. Seventy-two pigs were used, at 25.71 ± 7.51kg initial body weight (BW) and 98.81 ± 10.64kg final BW. The diets were formulated for the stages: 1 - 70 to 101; 2 - 102 to 130; 3 - 131 to 143 days of age. Because there was no redistribution of the animals in blocks at the beginning of stages 2 and 3, the data were evaluated at the periods: 1 - 70 to 101; 2 - 70 to 130; and 3 - 70 to 143 days of age. The inclusion of up to 15% of CP, with or without the addition of an enzyme complex in the diets, decreased the daily feed intake in Periods 1 and 2, and improved feed:gain in Period 2; however, the animals showed adaptive responses in Period 3. In the absence of the enzyme complex, the carcass yield and the fat/meat ratio decreased linearly as the levels of CP increased. In the presence of the enzyme complex, the fat/meat ratio had a quadratic behavior, and the level of 7.78% of CP determined the lowest ratio. The inclusion of up to 15% of CP with or without the addition of the enzyme complex in diets no differences in performance; therewith, it can be a feed alternative, without effect on the carcass characteristics.

Objetivou-se avaliar a produção de fezes+urina por animal e teores de matéria seca, matéria mineral, matéria orgânica, nitrogênio, fósforo, potássio e enxofre nas fezes de suínos alimentados com dietas contendo concentrações crescentes (0, 5, 10 e 15 mg kg-1 de dieta) de ractopamina. Foram utilizadas 468 fêmeas suínas, alojadas em 36 baias de piso compacto. A cada semana foram realizadas duas colheitas diárias de fezes e urina, de quatro baias por tratamento e duas colheitas semanais, de nove baias por tratamento, para determinação da composição fecal. Utilizou-se o delineamento em blocos, em esquema de parcelas subdivididas, sendo as parcelas as concentrações de ractopamina e as semanas como subparcelas. Não houve redução na quantidade de nitrogênio excretado. Observou-se interação entre concentração de ractopamina e semana, para a produção de fezes+urina por animal e para os teores de fósforo, potássio e enxofre nas fezes. A ractopamina na dieta reduz a produção de fezes+urina e a excreção de nitrogênio e fósforo nas duas semanas iniciais. Foram encontrados maiores valores estimados para o teor de potássio nas fezes de animais alimentados com dietas contendo 10 e 15 mg de ractopamina kg-1 entre a segunda e terceira semanas. O aumento na concentração de ractopamina de 5 até 15 mg kg-1 promove maior excreção de enxofre em função das semanas.

A study was conducted to evaluate the feces+urine produced per animal (FUPA), dry matter, mineral matter, organic matter, nitrogen, phosphorus, potassium and sulfur in feces of gilts fed diets with increasing levels of ractopamine (0, 5, 10 and 15 mg kg-1 of diet). A total of 468 finishing gilts were allotted into 36 pens. In two days of each week, feces and urine were daily sampled in four pens per treatment, quantifying the feces+urine. To determine the characterization of feces, two samples per week were taken daily, in nine pens per treatment. It was used a split plot design, considering the ractopamine level as the plot and the weeks as the subplots. There was no reduction in nitrogen amount in feces. An interaction was detected between ractopamine concentrations and weeks for FUPA and phosphorus, potassium and sulfur in feces. Ractopamine addition in diets for gilts has reduced the feces+urine production and nitrogen and phosphorus excretion. Higher values estimated for potassium content in feces of animals fed diets with 10 and 15 mg of ractopamine kg-1 were found between the second and third week. Increasing levels of ractopamine from 5 to 15 mg kg-1 promoted higher excretion of sulfur over the weeks of supply.

The objective of this study was to evaluate the effect of diets supplemented with plasma and/or yeast extract on performance (daily weight gain [DWG], daily feed intake [DFI] and feed conversion [FC]) and intestinal morphology of piglets from 7 to 63 days of age. A total of 288 piglets aged 7 days and weighing 2.57±0.05 kg were studied. A randomized block design consisting of four experimental diets, six repetitions and 12 piglets per experimental unit was adopted. The pre-starter I (7 to 21 days), pre-starter II (22 to 35 days) and starter I (36 to 49 days) diets were supplemented as follows: control diet (CD): no plasma or yeast extract; plasma (PL) diet: addition of 6%, 4% and 2% plasma; yeast extract (YE) diet: addition of 6%, 4% and 2% yeast extract; plasma + yeast extract (PL+YE) diet: addition of 3%, 2% and 1% plasma and yeast extract each. From 50 to 63 days of age all piglets received the same diet. No difference in performance was observed from 7 to 21 days and from 7 to 28 days of age, whereas DWG was higher from 7 to 35 days in piglets receiving the PL+YE diet (268, 278, 271 and 288 g/day for CD, PL, YE and PL+YE, respectively). From 7 to 49 days and from 7 to 63 days, DWG (330 and 519 g/day, respectively) and DFI (307 and 647 g/day) were higher in animals receiving the PL-YE diet when compared with those consuming CD (DWG: 295 and 486 g/day; DFI: 266 and 594 g/day). No significant differences in intestinal morphology were observed between piglets receiving the different diets. The combination of plasma and yeast extract elevates DWG, but does not affect the intestinal morphology of piglets from 7 to 63 days of age.

Os objetivos foram avaliar os efeitos da adição de diferentes concentrações de probiótico (0; 200 e 300mg/kg de ração) em rações de leitões recém desmamados e em diferentes idades de abate, sobre as características do sistema digestório e desempenho zootécnico. Foram utilizados 35 leitões desmamados, distribuídos em delineamento em blocos casualizados em esquema fatorial 3x2+1 (três concentrações de probiótico x duas idades de abate + grupo abatido ao desmame). Foram abatidos cinco leitões no dia da desmama e os demais ao 31º e 41º dia de idade. Foram amostrados segmentos do duodeno e jejuno para a contagem de vilosidades, pesados o pâncreas e fígado e calculado o rendimento de carcaça. Para incidência de diarréia e desempenho, foram utilizados 30 animais e as avaliações foram realizadas nos seguintes períodos: P1- 0 a 10, P2- 11 a 20 e Período Total- 0 a 20 dias pós desmame. Não houve efeito das concentrações do probiótico sobre as características do sistema digestório e a incidência de diarréia, no entanto o ganho de peso e a conversão alimentar foram afetados. Concluiu-se que o ganho de peso e a conversão alimentar de leitões suplementados com 200mg de probiótico/kg de ração foi melhor do que o grupo suplementado com 300mg de probiótico/kg de ração. As diferentes concentrações de probiótico adicionadas na ração de leitões não alteraram as características do sistema digestório, mostrando-se alterações apenas em relação à idade do abate.

The aims were to evaluate the effects of the addition of different probiotic concentrations (0; 200 and 300mg/kg of ration) in rations of pigs recently weaned and in different ages of slaughter, on the characteristics of the digesting system and performance. 35 weaned pigs were used, distributed in blocks randomized design in 3x2+1 (three probiotic concentrations x two ages of slaughter + group slaughter to the weaning). Five pigs were slaughter in the weaning day and the others to the 31 and 41 days of age. Were sampled segments of the duodenum and jejunum for counting villous, weighs the pancreas and liver and calculated the yield carcass. For diarrhea incidence and performance, 30 animals were used and the evaluations were accomplished in the following periods: P1 - 0 to 10, P2 - 11 to 20 and Total Period - 0 to 20 days after wean. There was not effect of the probiotic concentrations on the characteristics of the digesting system and the diarrhea incidence, however the weight gain and the feed:gain ratio were affected. The conclusion are: The gain weight and feed:gain ratio of piglets supplemented with 200mg of probiotic/kg of ration was better than the group supplemented with 300mg of probiotic/kg of ration. The different probiotic concentrations added in the piglets rations did not alter the characteristics of the digesting system, being showed alterations just in relation to the slaughter age.

This experiment was carried out to evaluate the effects of purified cellulose, soybean hulls and citrus pulp in the diet of weaned piglets. A total of 72 piglets (36 castrated males and 36 females) weaned at 21 days of age (BW 6.45±0.66 kg) was distributed according to a randomized block design, for evaluation of performance, transit time, diarrhea incidence and blood parameters of piglets which received diets containing different sources of fiber. The experimental diets were: control diet - diet composed of corn, soybean meal and a source of lactose; control + 1.5% purified cellulose; control + 3% soybean hull and control + 9% citrus pulp. The inclusion of purified cellulose, soybean hulls and citrus pulp in diets of weaned piglets did not affect the performance or transit time of diets in the gastrointestinal tract. However, the use of purified cellulose reduced incidence of diarrhea. The use of purified cellulose, soybean hull and citrus pulp in the diet of weaned piglets does not affect performance or gastrointestinal transit time. The addition of purified cellulose promotes a beneficial effect to control the diarrhea in weaned piglets.