ABSTRACT Piedras are small nodules found on the hair shafts. White piedra was initially described as caused by Trichosporon beigelii, which was later reclassified in several species. We describe the first case of white piedra caused by Cutaneotrichosporon debeurmannianum. Affected hairs were examined in natura with scanning electron microscopy, after gold metallization. The typical whitish cerebriform creamy colony was obtained in Sabouraud medium. Fungal genomic DNA extracted from cultures and locus was amplified with the NL1/NL4 primer pair from the D1/D2 region of the large ribosomal subunit (LSU) of 28S rRNA. With scanning electron microscopy, nodules are easily identified surrounding the hair shaft; with high magnifications, rounded structures adhered to each other were identified. Comparison of the nucleotide sequences of IMT-1703 Cutaneotrichosporon debeurmannianum revealed 99.6% similarity with the 28S large ribosomal unit rDNA sequence. This case of white piedra was caused by Cutaneotrichosporon debeurmannianum. shafts beigelii species microscopy metallization medium NL1NL4 NLNL NL1 NL4 NL NL1/NL D1D2 DD D1 D2 D D1/D LSU (LSU S rRNA shaft magnifications IMT1703 IMT 1703 IMT-170 996 99 6 99.6 sequence NL1NL D1D IMT170 170 IMT-17 9 99. IMT17 17 IMT-1 IMT1 1 IMT-

Abstract Background: Little is known about the ultrastructure of pili annulati. Objectives: To examine with transmission electron microscopy affected hairs of a family, whose diagnosis had been confirmed in five individuals with scanning electron microscopy, which showed surface undulations with ‘‘curtain-like’’ folding of the hair cuticula and to compare the findings with normal control. Methods: Hairs of two affected patients and one control were embedded in resin and cut lengthwise to produce ultra-thin sections. Results: The normal hair showed a parallel arrangement of dark lines associated with less electron-dense wide bands. Small cavities could be observed, mostly in the dark lines, affected hairs had a large number of cavities, associated or not with the insertion of melanosomes and loss of parallelism of the dark lines. Higher magnification showed a significant loss of this parallelism, resembling ‘‘wood grooves’’. Widened dark lines were observed in some areas. Study limitations: Only a few hairs were examined. Conclusions: The present results suggest that the microcanaliculi of the hair surface, easily found with scanning electron microscopy, may be secondary not only to the cavities seen in the sections but also to the disorder of proteins that form this region, demonstrated by the changes of the cortex dark lines.

Abstract Panitumumab is a monoclonal antibody against the epidermal growth factor receptor used in metastatic colorectal cancer; in addition to tumor cells, it acts on epidermal keratinocytes and on the outer root sheath and presents skin toxicity in up to 90% of cases. A scanning electron microscope was used to examine the eyelashes and hairs of a 65-year-old patient with eyelash trichomegaly, curly hair, and paronychia undergoing treatment with panitumumab. Grooving in the hair shafts were identified, which were more evident in the eyelashes. Similar to oral epidermal growth factor inhibitors (erlotinib and gefitinib), panitumumab can cause acquired pili canaliculi.

Abstract McCune - Albright syndrome is a genetic disease with cutaneous mosaicism caused by post-zygotic activating mutations in GNAS locus, it has a triad of fibrous bone dysplasia, café-au-lait macules and precocious puberty. We examined a 22-year-old female patient with café au lait spot in right side of the abdomen, with a chessboard - like distribution, extending to right thigh with geographical contours, she has also an ovarian cyst, scoliosis and truncal obesity. Biopsies were taken from the hyperpigmented area and processed for light microscopy and for transmission electron microscopy. Light microscopy showed increased melanin pigment with HE staining. Immunohistochemistry with melanocytic markers (HMB-45 and Melan-A) revealed a normal number of melanocytes. Transmission electron microscopy demonstrated normal epidermal structures, such as desmosomes, cytokeratin filaments and hemidesmosomes. With high magnifications an irregular melanossomal contour was seen, with some indentations in their outline.

Abstract A 73-year-old male patient developed a poorly differentiated squamous cell carcinoma in the anal canal nine months ago. He was treated with two cycles of 5-fluorouracil and cisplatin and concomitant radiotherapy (6 MeV linear photon accelerator, total dose of 54 Gy), with complete remission. Since forty-five days he presentes a painful perianal and intergluteal erosion with circinate pustular borders. Light microscopy showed pseudoepitheliomatous hyperplasia of the epidermis with microabscesses of inflammatory cells (neutrophils and eosinophils) and acantholytic keratinocytes . Indirect immunofluorescence was positive for IgG, with an intercellular pattern, 1:80 titer. The diagnosis of radiotherapy-induced pemphigus vegetans was established and there was significant regression with oral prednisone (40 mg) and topical betamethasone.

Abstract Lipoid proteinosis is a rare autosomal recessive disease, characterized by hyaline deposits of PAS-positive material in tissues due to mutations in the ECM1 gene. This study evaluated the ultrastructure of the skin of a 6-year-old child affected by this condition. The light microscopy identified PAS-positive hyaline deposits, which were more intense in the papillary dermis. Scanning electron microscopy of the dermis showed a compact papillary dermis and fibrillar deposits in the middle dermis. Transmission electron microscopy clearly showed the deposition of fibrillar material in the dermis, forming clusters adherent to elastic fibers, between the collagen bundles and the collagen fibers, and also filling up the cytoplasm of dermal fibroblasts.