Abstract This study aimed to evaluate volumetric polymerization shrinkage, degree of conversion and Vickers hardness of four bulk-fill resin composites light-activated with their dedicated light curing units (LCUs). Four groups were evaluated, according to the type of composite and curing mode: Tetric EvoCeram Bulk-fill (TEBO) and Tetric EvoFlow Bulk-fill (TEBF) were light-activated with Bluephase Style 20i (20s, in high-mode), while Tetric Powerfill (TEPO) and Tetric Powerflow (TEPF) were light-activated with Bluephase PowerCure (3s). Volumetric polymerization shrinkage test (n = 6) was performed in standardized box-shaped class-I cavities of extracted third molars (4 x 4 x 4 mm). Teeth were scanned before and after resin composite application by micro-computed tomography, and acquired data were evaluated with Amira software. Degree of conversion (n = 5) was evaluated at the top and bottom surfaces of composite cylindric samples (4 mm diameter, 4 mm thickness) using an FT-IR spectrometer (spectra between 1,500 and 1,800 cm-1, 40 scans at a resolution of 4 cm-1). Three Vickers indentations (50 g / 15 s), spaced 500 μm apart, were performed on the top and bottom composite surfaces and averaged. One-way ANOVA was used for data evaluation. TEPF showed the lowest volumetric polymerization shrinkage (p < 0.05), while the other composites were not significantly different within each other (p > 0.05). All materials presented a significant decrease in degree of conversion and Vickers hardness when compared top to bottom surfaces (p < 0.05). Bottom to top surface ratios for degree of conversion ranged from 0.8 (TEBO and TEPO) to 0.9 (TEBF and TEPF), and from 0.4 (TEPO) to 0.7 (TEBF and TEPF) for hardness. In conclusion, resinous materials present a decrease in hardness and degree of conversion from top to bottom even when a higher power is used, while the flowable material TEPF showed the lowest volumetric shrinkage values compared to the other materials. bulkfill bulk fill lightactivated activated LCUs. LCUs . (LCUs) mode Bulkfill Bulk TEBO TEBF i 20s, 20s s (20s highmode, highmode high , high-mode) TEPO (TEPO (TEPF 3s. 3s (3s) n 6 boxshaped box shaped classI class I ( mm. mm) microcomputed micro computed tomography software 5 diameter thickness FTIR FT IR spectra 1500 1 1,50 1800 800 1,80 cm1, cm1 cm 1, cm-1 cm1. cm-1) 50 (5 s, s) apart averaged Oneway One way evaluation p 0.05, 005 0.05 0 05 0.05) 0.05. 08 8 0. 09 9 TEPF, 04 07 7 conclusion (LCUs high-mode (3s 150 1,5 180 80 1,8 cm- 00 0.0 18

Resumo Estudos vêm demonstrando uma diminuição na resistência adesiva em dentina tratada com hipoclorito de sódio (NaOCl). O objetivo desse estudo foi avaliar o efeito do plasma de argônio não-térmico na resistência de união de um sistema adesivo autocondicionante à dentina exposta ao NaOCl. Trinta e duas superfícies dentinárias lisas de incisivos bovinos foram imersas em NaOCl a 2,5% por 30 min para simular o passo de irrigação durante o tratamento endodôntico. Os espécimes foram divididos em 4 grupos (n=8), de acordo com o tratamento de superfície: Controle (sem tratamento de plasma), AR15 (plasma de argônio por 15 s), AR30 (plasma de argônio por 30 s) e AR45 (plasma de argônio por 45 s). Para teste de resistência de união por microtração, cinco espécimes foram utilizadas por grupo. Em cada grupo, os espécimes foram hibridizados com um sistema adesivo autocondicionante (Clearfil SE Bond, Kuraray) e blocos de resina composta foram construídos. Após 48 h de armazenamento em água, os espécimes foram seccionados em palitos (5 por dente - 25 por grupo) e submetidos ao teste de resistência de união por microtração (μTBS) até a fratura, avaliando o padrão de fratura. Três amostras por grupo foram analisadas sob espectroscopia por FTIR para verificar as modificações químicas produzidas pelos tratamentos na dentina. Os dados de microtração foram avaliados estatisticamente utilizando os testes de ANOVA e Tamhane (p<0,05). AR30 apresentou o maior μTBS (20,86±9,0). AR15 (13,81±6,4) e AR45 (11,51±6,8) foram estatisticamente semelhantes ao controle (13,67±8,1). A espectroscopia por FTIR mostrou que o tratamento de plasma produziu modificações químicas na dentina. Como conclusão, o tratamento de plasma de argônio não-térmico por 30 s produziu alterações químicas na dentina e melhorou o μTBS do Clearfil SE Bond à dentina tratada com NaOCl.

Abstract Studies have been showing a decrease of bond strength in dentin treated with sodium hypochlorite (NaOCl). The aim of this study was to evaluate the effect of non-thermal argon plasma on the bond strength of a self-etch adhesive system to dentin exposed to NaOCl. Thirty-two flat dentin surfaces of bovine incisors were immersed in 2.5% NaOCl for 30 min to simulate the irrigation step during endodontic treatment. The specimens were divided into four groups (n=8), according to the surface treatment: Control (without plasma treatment), AR15 (argon plasma for 15 s), AR30 (argon plasma for 30 s) and AR45 (argon plasma for 45 s). For microtensile bond strength test, 5 specimens were used per group. In each group, the specimens were hybridized with a self-etch adhesive system (Clearfil SE Bond) and resin composite buildups were constructed. After 48 h of water storage, specimens were sectioned into sticks (5 per tooth, 25 per group) and subjected to microtensile bond strength test (μTBS) until failure, evaluating failure mode. Three specimens per group were analyzed under FTIR spectroscopy to verify the chemical modifications produced in dentin. μTBS data were analyzed using ANOVA and Tamhane tests (p<0.05). AR30 showed the highest μTBS (20.86±9.0). AR15 (13.81±6.4) and AR45 (11.51±6.8) were statistically similar to control (13.67±8.1). FTIR spectroscopy showed that argon plasma treatment produced chemical modifications in dentin. In conclusion, non-thermal argon plasma treatment for 30 s produced chemical changes in dentin and improved the μTBs of Clearfil SE Bond to NaOCl-treated dentin.