Abstract This study presents geophysical data from two passive seismic measurements conducted at two different sites in Antarctica. We analyzed the signals mainly in the frequency domain through the multitaper method to extract some spectral characteristics of the signals that would have been out of reach through the usual FFT approach. The power spectral density of the signals carries information about the processes that generated them, allowing its correlation with their source origin and type, either natural or anthropogenic. We deal with three different source types: calving, wind, and anthropogenic origins. The former is closely related to glacier dynamics, being modulated by the prevailing atmospheric processes. At both locations the wind noise is prevalent, complicating the analysis of other events like calving. We have used data classification, estimation of the source azimuth, and seismic apparent velocity to demonstrate the viability of using geophysical methods to study glacier elastic parameters and dynamics. Moreover, the calving rate can yield a wider and more independent understanding of glacier hydrodynamics and may help to estimate the future response of the polar areas to a changing environment. Antarctica approach them type types origins dynamics prevalent classification azimuth Moreover environment

Abstract Biomphalaria straminea is one of the three snails that act as the intermediate hosts of Schistosoma mansoni and is responsible for maintaining high rates of schistosomiasis in some areas of northeastern Brazil. The principal morphological characteristic of B. straminea is the marked vaginal wrinkling, although it is also present in congeneric species, a group known as the B. straminea complex. Based on the morphological and molecular analyses, we investigated the intraspecific variation of B. straminea. The specimens were obtained from 10 sites in Brazil, and the shells were analyzed, as well as 16 morphological structures of the reproductive system. The COI and ITS2 sequences were used for phylogenetic analysis, genetic divergence, and haplotype network (COI). We observed a large intraspecific variation in the morphological structures examined. The genetic divergence also demonstrated significant intraspecific variability in B. straminea: 0–3% in ITS2 sequences, and 0–6% in COI sequences. Analysis of the distribution of COI haplotypes recovered 16 haplotypes and haplotype diversity of 0.9088. These results indicate phenotypic variability that is not constrained to a locality or strictly controlled genetically by B. straminea, which may have driven a misidentified of these species. Brazil B wrinkling species complex analyses 1 analyzed system ITS analysis COI. . (COI) examined 03 0 3 0–3 06 6 0–6 09088 9088 0.9088 (COI 0– 0908 908 0.908 090 90 0.90 09 9 0.9 0.

Abstract The demand for less hazardous and environmentally friendly surface disinfectants is growing and essential oils may be efficient alternatives. Vapor phase assay is a promising technique which explore the volatility of essential oils for determining the antimicrobial activity. In this study, we modified the conditions of the inverted Petri dish technique to evaluate the minimum inhibitory concentrations (MIC). As a proof of concept, the antimicrobial activity of Hesperozygis myrtoides essential oil (HM) was studied for the first time in the vapor phase. According to the established protocol the oil volatilization dynamics was used to determine the major compounds in the vapor phase. After 270 min, the relative percentage was like that observed in the crude oil analysis. The HM was also evaluated for safety (IC50=921.2 µg ml-1), being considered safe in case of acute oral exposure. The oil vapors completely inhibited the growth of Staphylococcus aureus (MIC=0.39 mg l-1), Candida albicans (MIC=0.83 mg l-1), Aspergillus brasiliensis (MIC=378 mg l-1) and Bacillus subtilis (MIC=704.8 mg l-1). This protocol offers an economical and efficient alternative for antimicrobial activity and safety determination of essential oils, can be used for the evaluation of several applications of essential oils, as disinfectant agent in inhabited environments. alternatives study MIC. MIC . (MIC) concept (HM 27 min analysis IC50=921.2 IC509212 IC IC50 921 2 (IC50=921. ml1, ml1 ml 1 , ml-1) exposure MIC=0.39 MIC039 0 39 (MIC=0.3 l1, l1 l MIC=0.83 MIC083 83 (MIC=0.8 MIC=378 MIC378 378 (MIC=37 l-1 MIC=704.8 MIC7048 704 8 (MIC=704. l1. environments (MIC IC50=921. IC50921 IC5 92 (IC50=921 ml-1 MIC=0.3 MIC03 3 (MIC=0. MIC=0.8 MIC08 MIC=37 MIC37 37 (MIC=3 l- MIC=704. MIC704 70 (MIC=704 IC50=921 IC5092 9 (IC50=92 ml- MIC=0. MIC0 (MIC=0 MIC=3 MIC3 (MIC= MIC=704 MIC70 7 (MIC=70 IC50=92 IC509 (IC50=9 MIC=0 MIC= MIC=70 MIC7 (MIC=7 IC50=9 (IC50= MIC=7 IC50= (IC50 (IC5 (IC

Abstract The use of marine microorganisms in the treatment of dyes and textile effluents is promising in view of their tolerance to salinity, a characteristic found in this kind of effluent. In this study, different culture conditions were applied to evaluate the decolorization, degradation, and detoxification of Sulphur Indigo Blue (SIB) by the marine-derived basidiomycete Paramarasmius palmivorus CBMAI 1062. Low salt concentration (SLS) and high salt concentration (SMASHS) media were used. P. palmivorus decolorized 100 % and 91.38 % of SIB after 120 h of growth in the SLS medium and after 168 h of growth in the SMASHS medium, respectively. Laccase activity was detected only in the SLS bioassay. UV-Vis, FT-IR, and GC-MS analyses indicated the occurrence of dye biosorption and biotransformation. In the SLS medium metabolites associated with SIB biotransformation (e.g. aldehyde, alkanes, and phenols) were detected. The toxicity measured by Cucumis sativus decreased from 45.41 % to 24.11 % in the SLS bioassay, while in SMASHS medium there was no change in toxicity. The efficiency for decolorization and detoxification of SIB indicates that microorganisms from the marine environment can be a source for biotechnological application in bioremediation processes carried out under saline conditions, adding value to blue biotechnology. salinity effluent study degradation (SIB marinederived derived 1062 (SLS (SMASHS used P 10 9138 91 38 91.3 12 16 respectively bioassay UVVis, UVVis UV Vis, Vis UV-Vis FTIR, FTIR FT IR, IR FT-IR GCMS GC MS e.g. eg e g (e.g aldehyde alkanes phenols 4541 45 41 45.4 2411 24 11 24.1 biotechnology 106 1 913 9 3 91. e.g 454 4 45. 241 2 24.

Abstract This study explored the association of cardioprotective role of thyroid hormones (TH) and benefit effects of grape juice (GJ) on vascular function against the apoptosis and inflammation associated with monocrotaline (MCT)-induced pulmonary hypertension (PH). Wistar rats were distributed into five groups: control; PH (MCT 60mg/kg i.p.); PH+GJ (GJ-7 µL/g / day, gavage for 14 days); PH+TH (T3, 2μg/100g/day, and T4, 8μg/100g/day, by gavage for 14 days); and PH+TH+GJ. Echocardiographic, morphometric measurements and expression of proteins associated with apoptosis and inflammation were evaluated. The reduced cardiac output (35%) in PH was attenuated in the PH+GJ, PH+TH, and PH+TH+GJ groups (P<0.05). Both GJ and TH had no effect on the PH-induced hypertrophy index. The PH + TH group showed reduced BAX (~90%) and increased Bcl2 levels (~80%) as compared to PH group (P<0.05). The PH-induced increase in caspase 3 was diminished (~92%) in the PH+TH (P<0.05). Both GJ and TH, isolated and combined, reduced caspase 9 levels (~70%). PH-provoked elevated NLRP3 levels was decreased (~90%) in PH+TH (P<0.05). The therapeutic approach showed cardioprotective effects in the PH model which may be mediated by mitigation of apoptosis and inflammation. (TH (GJ MCTinduced MCT induced PH. . (PH) control 60mgkg mgkg 60mg kg mg i.p. ip i p i.p.) PHGJ GJ7 7 (GJ- µLg µL g day 1 days days) PHTH T3, T3 T (T3 2μg100gday μggday 2μg 100g μg 2μg/100g/day T4 8μg100gday 8μg 8μg/100g/day PHTHGJ Echocardiographic evaluated 35% 35 (35% P<0.05. P005 P P<0.05 0 05 (P<0.05) PHinduced index ~90% 90 (~90% Bcl ~80% 80 (~80% ~92% 92 (~92% combined ~70%. 70 ~70% (~70%) PHprovoked provoked NLRP (PH i.p (T gday (35 P00 P<0.0 (P<0.05 ~90 (~90 ~80 8 (~80 ~92 (~92 ~70 (~70% (3 P0 P<0. (P<0.0 ~9 (~9 ~8 (~8 ~7 (~70 ( P<0 (P<0. ~ (~ (~7 P< (P<0 (P< (P

Abstract Effective freight transportation planning through different modes drives countries economic growth by ensuring the delivery of goods safely and sustainably. This article provides a systematic literature review focused on intermodal transport tactical planning, addressing logistical technical factors for efficient transportation modes management in cargo delivery. The methodology is based on the research questions definition, search process, and article selection criteria, exploring the network structures evolution to verify technical factors influencing network structures, including mode combinations, geographical distribution, and mathematical models’ decision variables and constraints. According to the research question proposed, the most relevant technical factors for intermodal planning network structures are flow cargo, transport modal activation, flow conservation, delivery time, and vehicle management, generally found in countries with extensive road infrastructure and a high modal integration capacity, as China, Europe, and USA. Considering the decision variables and constraints, flow conservation is a common element observed in intermodal transport mathematical models. Thus, a balance between transportation costs and transportation modes utilization was verified, and insights into the relation between technical logistics factors application and network structures for cargo delivery efficiency were revealed. Finally, the systematic literature review contributes to understanding the main intermodal transportation planning evolving landscape, offering valuable insights for future research and practical implementations. sustainably definition process criteria combinations distribution models constraints proposed activation time capacity China Europe USA Thus verified revealed Finally landscape implementations

Abstract To understand the ecoepidemiology of fleas and their interactions with domestic and wild animals, it is necessary to decode the cycles of flea abundance in relation to host species and environmental factors. The province of Misiones, known for its biodiversity and land use changes, provides an excellent study location. We assessed the diversity, prevalence, abundance, and parasitic intensity of fleas collected from 1153 rodents and opossums in various environments in the Upper Paraná Atlantic Forest of Argentina, considering phytogeography and land use. Our findings were consistent with previous studies conducted in humid regions of Argentina and the Atlantic Forest in Brazil. Hosts with high population density exhibited greater flea prevalence and abundance, and widely distributed hosts showed higher flea species richness and diversity. Significant similarities in flea similarity indices among phytogeographic areas, especially in forest. Environments with an anthropogenic gradient exhibited marked dissimilarities, with higher species richness and diversity in less disturbed sites. These parameters may be influenced by the presence of polyxenous Ctenocephalides, Polygenis, and Adoratopsylla fleas co-infesting opossum in urban areas. These findings provide valuable insights into the ecoepidemiology of fleas, their relationships with host species, and the impact of environmental factors in a strategic Atlantic forest ecoregion. animals Misiones changes location 115 Brazil areas dissimilarities sites Ctenocephalides Polygenis coinfesting co infesting ecoregion 11 1

Abstract Fewer donations are being made in Brazil to meet the growing organ demand. Organ donation in Brazil reached an average of 53% consent. However, hospitals in Paraná have reached a level of 94.2%. What reasons could be given for these levels? Accordingly, this study aimed to understand the causes involved in decision-making to donate organs. The methodology used was qualitative based on a case study. Data was collected at a hospital in Toledo, a city in Paraná, through documentary research and semi-structured interviews with two distinct groups: professionals responsible for the family approach to donation and five families consenting to donation. The search for data was restricted to the period between 2015 and 2023. Data analysis used Bardin’s content analysis. The results were organized into four categories in the first group, and two categories in the second group, suggesting that aspects linked to bioethical references present in the interview, such as beneficence and autonomy, contribute to the emergence of high rates of family consent for organ donation in the hospital studied. It is recommended for future research to test successful interview models to reverse the current organ donation rates in Brazil. demand 53 However 942 94 2 94.2% levels Accordingly decisionmaking decision making organs Toledo semistructured semi structured groups 201 2023 Bardins Bardin s group autonomy studied 5 9 94.2 20 202 94.

Abstract Floodwater mosquitoes provoke nuisance due to their mass emergence. Citizen complaints about outstanding events of mosquitoes in urban areas are usually reflected in local media and could be potentially used as data. Our objective was to build a temperature dependent function to characterize the immature development time of the floodwater mosquito Ae. albifasciatus in Tandil (Argentina), and validate it using citizen complaints in local media and field collected mosquitoes. The development time obtained ranges from 5-47 days at 30-7°C, respectively. During 2000-2021, nine mosquito abundance peaks were identified through local journalistic records. For these events, the estimated hatching date was successfully associated with a previous precipitation, with a maximum difference of two days. The precipitation identified as the trigger for egg hatching ranged from 15 to 121 mm. Then, to associate mosquito news with the target species, female mosquitoes were captured by a battery-powered aspirator in green areas of the city during September 2019-June 2021. Six mosquito abundance peaks were detected in which Ae. albifasciatus accounted for 97.3% of the captures. Among them, journalistic records were able to detect the largest two; i.e. in our study above 15 mosquitoes in a five minute collection. Citizen complaints related to nuisance or vector species could be valuable retrospective data for researchers of different fields. emergence Ae Argentina, Argentina , (Argentina) 547 5 47 5-4 307°C, 307C C 30 7°C, 7 30-7°C respectively 20002021, 20002021 2000 2021, 2021 2000-2021 1 12 mm Then batterypowered battery powered 2019June June 2019 973 97 3 97.3 captures them ie i e i.e collection fields (Argentina 54 4 5- 307 307°C 7C 7°C 2000202 200 202 2000-202 201 9 97. 200020 20 2000-20 20002 2 2000-2 2000-

Abstract We examined pesticide contamination in honey from Africanized honey bees in two different seasons, and evaluated the concentration and incidence of these compounds in georeferenced apiaries to provide relevant information for food safety and environmental quality. The land use and occupation of the region were evaluated by selecting 15 apiaries and georeferencing three randomly selected colonies of Africanized honey bees within each apiary. Honey samples were collected during two seasons, and three organochlorine pesticides (p.p`-DDE, Mitotane and Bicyclo[2.2.1]) and four organophosphate pesticides (Ethoprophos, Methyl Parathion, Chlorpyrifos and Fenchlorphos) were analyzed in the honey using gas chromatography-mass spectrometry. 80% of the apiaries showed a predominance of agricultural land use in the foraging areas of the bees. There was a higher concentration of pesticides in samples collected during Season I compared to Season II. There were differences in the proportion of samples contaminated with pesticides between seasons. Some honey samples showed concentrations of the chlorpyrifos pesticide above the levels recommended by Brazilian legislation. Pesticide contamination of honey is not limited to specific application season, indicating the persistent presence and easy availability of pesticides, including banned pesticides not sold or prescribed by agronomists, potentially smuggled from neighboring countries. seasons quality 1 apiary p.p`DDE, ppDDE p.p` DDE, p DDE (p.p`-DDE Bicyclo2.2.1 Bicyclo221 Bicyclo 2.2.1 2 Bicyclo[2.2.1] Ethoprophos, Ethoprophos (Ethoprophos Parathion Fenchlorphos chromatographymass chromatography mass spectrometry 80 II legislation season agronomists countries p.p`DDE pp p.p Bicyclo2 Bicyclo2.2. Bicyclo22 221 2.2. Bicyclo[2.2.1 8 Bicyclo2.2 22 2.2 Bicyclo[2.2. Bicyclo2. 2. Bicyclo[2.2 Bicyclo[2. Bicyclo[2 Bicyclo[

Abstract The fruit of Spondias mombin (cajazeira) is widely consumed in the northeast region of Brazil. In this work, three different extraction methods, namely ultrasound-assisted extraction (UAE), turbo-extraction (TE) and pressurized hot water extraction (PHWE), were evaluated in order to investigate the potential of cajazeira leaves as an alternative source of bioactive compounds. The extraction methods were compared in terms of yield, chemical composition and total phenolic content (TPC) of the extracts. The highest yields and TPC values were obtained by TE with a mixture ethanol: water (70:30%, v/v) as the solvent. PHWE has not yet been applied for the extraction of cajazeira leaves. Thus, it was evaluated as one alternative for the recovery of phenolic compounds under conditions of 80, 100 and 120 °C and 100 and 120 bar. A modeling study of PHWE kinetics was investigated at 80 °C and 200 bar. Furthermore, a biorefinery approach considering integrated processes to recover bioactive compounds was investigated and the results showed that combining processes may enhance the valorization of agricultural waste. (cajazeira Brazil work ultrasoundassisted ultrasound assisted UAE, UAE , (UAE) turboextraction turbo (TE PHWE, (PHWE) yield (TPC extracts ethanol 7030%, 7030 70 30%, 30 (70:30% v/v vv v solvent Thus 10 12 C bar 8 20 Furthermore waste (UAE (PHWE 7030% 703 7 30% 3 (70:30 1 2 (70:3 (70: (70 (7 (

Abstract The Sarcophagidae family (Diptera) encompasses a group of flies of significant ecological importance. Additionally, numerous species within this family hold substantial value in the realms of medicine, veterinary and forensic science. The objective of this study was to conduct a comprehensive survey of Sarcophagidae species within transitional zones between the Caatinga and Cerrado biomes in the Northeastern region of Brazil. To achieve this, a total of 15 field expeditions were conducted across three distinct phytophysiognomies—riparian, vereda, and a segment of shrubby Caatinga—from 2019 to 2021. A total of 16 genera and 40 species were collected. Among them, a new species for science (Titanogrypa (Airypel) sp. nov.), Emblemasoma emblemasoma Dodge is newly recorded from South America/Brazil and the Caatinga biome, and three species constitute new records for the Northeast region (Dexosarcophaga patiuorum Santos, Pape & Mello-Patiu, Lepidodexia (Notochaeta) fumipennis (Lopes), and Oxysarcodexia meridionalis (Engel)). The most abundant species were Oxysarcodexia thornax (Walker), Oxysarcodexia avuncula (Lopes), and Argoravinia (Argoravinia) catiae Carvalho Filho & Esposito, collectively constituting 56.11% of the entire collected sample. The distribution of species across different vegetations was noted, with 19 species exclusive to riparian vegetation, three to vereda vegetation, and seven species unique to the Caatinga. Diptera (Diptera importance Additionally medicine Brazil 1 phytophysiognomiesriparian, phytophysiognomiesriparian phytophysiognomies riparian, phytophysiognomies—riparian Caatingafrom 201 2021 4 them Titanogrypa Airypel (Airypel sp nov., nov nov. , nov.) AmericaBrazil America biome Dexosarcophaga Santos MelloPatiu, MelloPatiu Mello Patiu, Patiu Mello-Patiu Notochaeta (Notochaeta Lopes, Lopes (Lopes) Engel. Engel . (Engel)) Walker, Walker (Walker) (Argoravinia Esposito 5611 56 11 56.11 sample noted vegetation 20 202 (Lopes (Engel) (Walker 561 5 56.1 2 (Engel 56.

Abstract Hepatic fibrosis is characterized by excessive deposition of collagen in the hepatic parenchyma, which disturbs the normal architecture and function. We have shown that human amniotic membrane (AM) can be used as a patch on the whole liver surface, resulting in an extremely significant reduction in collagen deposition. The aim of this study was to investigate the effects of AM on the matrix metalloproteinase 9 (MMP9) and matrix metalloproteinase 12 (MMP12) genes and proteins expression by real time quantitative PCR and immunohistochemistry, respectively, as well as image analysis on biliary fibrosis induced in rats by the bile duct ligation (BDL).Two weeks after the BDL, an AM fragment was applied onto the liver, and four weeks later, the liver samples were collected. MMP9 and MMP12 genes were significantly over expressed in group treated with AM. The immunoexpression of MMP9 and MMP12 was observed in all groups. However, the quantitative image analysis demonstrated an increase of the area occupied only by MMP12 in the livers of AM-treated rats with respect to BDL rats. These findings suggest that the AM exerts its beneficial effects on biliary fibrosis by increasing the MMP12, which in turn reduces the excessive collagen deposition on liver tissue. parenchyma function (AM surface MMP (MMP9 1 (MMP12 immunohistochemistry respectively BDL.Two BDLTwo .Two Two later collected MMP1 groups However AMtreated tissue (MMP (MMP1

Abstract Accurate wind power prediction can effectively alleviate the pressure of the power system peak frequency regulation, and is more conducive to the economic dispatch of the power system. To enhance wind power forecasting accuracy, a hybrid approach for wind power interval prediction is proposes in this study. Firstly, an Improved Complete Ensemble Empirical Mode Decomposition with Adaptive Noise (ICEEMDAN) is applied to decompose the initial wind power sequence into multiple modes, and Variational Mode Decomposition is used to further decompose the high-frequency non-stationary components. Next, Fuzzy Entropy (FE) is utilized to assess the complexity of the post-decomposed Intrinsic Mode Functions (IMFs), and different forecasting methods are employed accordingly, the point predictions were obtained by linearly summing the component predictions.Additionally, an improved sparrow search algorithm (ISSA) is used to seek the optimal hyperparameters of the prediction algorithm. Finally, the prediction intervals are constructed using the point prediction results based on kernel density estimation (KDE). The root mean square errors (RMSE) of deterministic predictions are 2.8458 MW and 1.8605 MW, with uncertainty coverage rates of 95.83% and 97.92% at a 95% confidence level. regulation accuracy study Firstly ICEEMDAN (ICEEMDAN modes highfrequency high nonstationary non stationary components Next FE (FE postdecomposed post decomposed IMFs, IMFs , (IMFs) accordingly predictionsAdditionally Additionally predictions.Additionally ISSA (ISSA Finally KDE. KDE . (KDE) RMSE (RMSE 28458 2 8458 2.845 18605 1 8605 1.860 9583 95 83 95.83 9792 97 92 97.92 level (IMFs (KDE 2845 845 2.84 1860 860 1.86 958 9 8 95.8 979 97.9 284 84 2.8 186 86 1.8 95. 97. 28 2. 18 1.

Abstract Currently, there is a demand for protein sources that do not use soil management or animal breeding. Among these sources we highlight the microorganisms, such cyanobacteria and microalgae, which have a simple growth using light, CO2, water and some mineral salts to generate high protein production. The extraction of these proteins depends on the method used. The most used methods for extracting bio-functional proteins are mechanical, chemical and enzymatic. The aim of this work is to analyze the protein extraction methods in microalgae using Scielo, ScienceDirect and NCBI (PubMed) electronic databases that made it possible to select original studies published in the last five years (2018-2023). A total of 2707 articles, 25 of which were selected for further analysis and subjected to risk of bias assessment. The genera Chlorella, Scenedesmus and Nannochloropsis were the most studied due to their high protein content. Mechanical methods and chemical hydrolysis are the most used methods, achieving an extraction yield of 46.0 % and 64.0 %, respectively. The best extraction results are obtained with a combination of methods, reaching up to 80.0 % yield. However, some aspects need to be observed to choose an ideal protein extraction method. Currently breeding microorganisms light CO2 CO production biofunctional bio functional mechanical enzymatic Scielo PubMed (PubMed 20182023. 20182023 2018 2023 . (2018-2023) 270 articles 2 assessment Chlorella content 460 46 0 46. 640 64 64. respectively 800 80 80. However 2018202 201 202 (2018-2023 27 4 6 8 201820 20 (2018-202 20182 (2018-20 (2018-2 (2018- (2018 (201 (20 (2 (