ABSTRACT. This study aimed to evaluate the nutrient intake, milk production, and production costs of dairy goats fed diets containing the rations with cactus cladodes (Opuntia stricta or Nopalea cochenillifera) associated with silage (Sorghum bicolor) or Tifton hay (Cynodon spp). Twelve pluriparous Saanen and Anglo Nubiana goats (42.25 ± 5.48 kg) at eight weeks of lactation were used. Treatments lasted 60 days, consisting of four periods of 15 days, distributed in three Latin square (4×4) with four diets. The treatments represented by diets with different associated feed: SSOP (Sorghum Silage + Opuntia); THOP (Tifton hay + Opuntia); SSNO (Sorghum Silage + Nopalea); and THNO (Tifton hay + Nopalea), in addition to concentrate in all diets. There were differences (p < 0.05) for the intakes of DM, OM, with higher values observed for goats fed the diets THNO, THOP, and SSNO, and for CP, NDF and ADF were higher for THNO and SSNO diets. Similar behavior occurred for voluntary and total water intake. The milk production and feed efficiency did not differ (p > 0.05). Costs with food, milk revenue, and gross revenue were not influenced by diets (p > 0.05). The Opuntia or Nopalea cactus cladodes genotypes associated with sorghum silage or tifton hay can be used to feed dairy goats in the semiarid region. ABSTRACT intake cochenillifera Sorghum bicolor Cynodon spp. spp . spp) 42.25 4225 42 25 (42.2 548 5 48 5.4 kg 6 days 1 4×4 44 4 (4×4 Opuntia) Nopalea) Nopalea, , p 0.05 005 0 05 DM OM CP 0.05. food region 42.2 422 2 (42. 54 5. 4× (4× 0.0 00 42. (42 (4 0. (

ABSTRACT. Twenty-four lambs between four and five months of age were divided into sex classes: uncastrated males, castrated males and females, eight for each treatment, with an average body weight of 27.00 ± 3.13 kg. dry matter and water intakes were higher for uncastrated males (p < 0.001). final body weight, body weight at slaughter, average daily gain and cold carcass weight were greater for uncastrated, intermediate for castrated and lower for females (p < 0.05). hot and cold carcass yields were higher for castrated males and females, respectively. (p < 0.05). based on principal component analysis, twenty-five variables were selected out of thirty-two, in some of the seven principal components generated, summarizing 21.87% data dimension. Discriminant analysis identified greater discrimination power for body weight at slaughter, thorax width, thorax depth, fatness, dry matter intake and hot carcass weight. All lambs were classified into their respective sex classes. Therefore, uncastrated males have performed better than castrated ones and both showed higher performance than females. Females and castrated males showed higher carcass yield. Discriminant analysis indicated heterogeneity between sex classes. ABSTRACT Twentyfour Twenty classes treatment 2700 27 00 27.0 313 3 13 3.1 kg p 0.001. 0001 0.001 . 0 001 0.001) slaughter 0.05. 005 0.05 05 0.05) respectively twentyfive twenty thirtytwo, thirtytwo thirty two, two thirty-two generated 2187 21 87 21.87 dimension width depth fatness Therefore yield 270 2 27. 31 1 3. 000 0.00 0.0 218 8 21.8 0. 21.

The purpose of the study was to calculate the effects of urban untreated wastewater irrigation on the heavy metals accumulation in vegetable samples consisting turnip (Brassica rapa), radish (Raphanus sativus), spinach (Spinacia olerácea), pumpkin (Cucúrbita moschata), cabbage (Brassica oleracea capitata), onion (Allium cepa), smooth luffa (Luffa aegyptiaca), coriander (Coriandrum sativum), vegetable gourd (Lagenaria siceraria) and carrot (Daucus carota subsp. sativus). This was accomplished by monitoring waste as well as fresh water-irrigated agricultural fields of Lakki Marwat, Pakistan. Flame atomic absorption spectrometer (FAAS) was used to assess the concentrations of these heavy metals. The concentration of a total of six metals including iron (Fe), zinc (Zn), copper (Cu), nickel (Ni), lead (Pb), and cadmium (Cd) were detected and quantified in vegetable samples. Concentration of Pb was very low, while Fe concentration was higher in all the samples under investigation in the waste water-irrigated vegetables. The highest concentrations of Zn were observed in onion (441.3±2.7 mg.kg-1), and smooth luffa (293.1±0.5 mg.kg-1). Onion (361.95±2.3 mg.kg-1), turnip (292.15±5.6 mg.kg-1) and Spinach (280.25±4.8 mg.kg-1) showed exceedingly higher concentrations of Fe. Daily intakes of metals (DIM) were calculated for both adults as well as children. The DIM values for Ni and Cd exceeded the recommended value. The concentration of Fe and Zn was higher in wastewater-irrigated area samples as compared to fresh water-irrigated vegetables. The data obtained displayed a trend of heavy metal Fe>Zn>Cu>Ni>Cd>Pb. Our findings have demonstrated the accumulation of heavy metals in the vegetables irrigated by the wastewater as confirmed by flame atomic absorption spectroscopy.

ABSTRACT Background: The increasing evidence of risk to the environment and human health by cyanotoxin exposure during cyanobacterial blooms has been reported worldwide. Despite the knowledge of cyanotoxin presence in Latin America, cyanotoxin bioaccumulation from freshwater environments have not been reviewed for the region. Goals: To review the current knowledge of cyanotoxin accumulation in tissues of freshwater organisms in field studies in Latin America. Methods: An extensive literature search was conducted to construct a database including information on accumulation of cyanotoxins in organisms inhabiting freshwater environments in Latin America (i.e., México to Argentina). Results: We found twenty-one studies from 2001 to 2020, including twenty-seven mostly eutrophic water bodies, the majority from Brazil. Microcystis was the most reported genus responsible for cyanotoxin production. Fish comprised most of the species accumulating cyanotoxins (20 species). Nile tilapia (Oreochromis niloticus) was the most studied species, and 80% of the fish species included have commercial importance, which highlights a potential route of exposure to humans by consumption of contaminated food. Some studies showed the reduction of cyanotoxins in tissues after an experimental depuration time. Also, calculations of the potential human intakes of microcystins by fish consumption exceeded the recommendations of tolerable intakes in most of the cases. Conclusions: In Latin America, the geographic extent of studies is narrow, however the summarized information indicates a risk for environment and human health by cyanotoxins bioaccumulation. There is a need for more efforts to generate scientific research on cyanotoxins bioaccumulation, but also for improvement of local level management policies to reduce eutrophication.

RESUMEN Antecedentes: La creciente evidencia de riesgo para el medio ambiente y la salud humana por la exposición a cianotoxinas durante las floraciones de cianobacterias se ha reportado en todo el mundo. A pesar del conocimiento de la presencia de cianotoxinas en América Latina, la bioacumulación de cianotoxinas en ambientes de agua dulce no ha sido revisada para la región. Objetivos: Revisar el conocimiento actual sobre la acumulación de cianotoxinas en tejidos de organismos de agua dulce en estudios de campo en América Latina. Métodos: Se realizó una extensa búsqueda bibliográfica para construir una base de datos que incluyera información sobre la acumulación de cianotoxinas en organismos que habitan ambientes de agua dulce en América Latina (de México a Argentina). Resultados: Encontramos veintiún estudios de 2001 a 2020, incluidos veintisiete cuerpos de agua en su mayoría eutróficos, la mayoría de Brasil. Microcystis fue el género productor de cianotoxinas más reportado. Los peces comprendieron la mayoría de las especies que acumulaban cianotoxinas (20 especies). Oreochromis niloticus fue la especie más estudiada, y el 80% de las especies de peces incluidas tienen importancia comercial, lo que destaca una vía potencial de exposición a los humanos. Algunos estudios demostraron la reducción de cianotoxinas en los tejidos después de un tiempo de depuración experimental. Además, los cálculos de las ingestas humanas potenciales de microcistinas por consumo de pescado excedieron las recomendaciones de ingestas tolerables en la mayoría de los casos. Conclusiones: En América Latina, los estudios se han realizado en pocos países, sin embargo, estos trabajos indican un riesgo para el medio ambiente y la salud humana por la bioacumulación de cianotoxinas. Se necesita más investigación científica sobre la bioacumulación de cianotoxinas, pero también esfuerzos para mejorar la gestión a nivel local con la finalidad de reducir la eutrofización.

ABSTRACT In a randomized controlled trial, we evaluated the effects of five oral electrolyte solutions (OESs) with different compositions of water, electrolyte, and acid-base balances of diarrheal neonatal calves. Osmotic diarrhea and dehydration were induced with sucrose in milk, spironolactone, and hydrochlorothiazide for 48 h in thirty 10-day old Holstein healthy calves with 43.5 ± 3.80 kg BW who were fed with natural milk. They were allocated to five treatment groups (n=6) based on the administered OES (commercial: OES A, B, C, D; and non-commercial: OES UEL). On the day of treatment, the calves received 6L of OES in two doses apart from milk intakes. Venous blood samples were collected at -48h (start of induction), -24h, 0h (start of the treatment day), 8h, 16h, 24h, 48h, and 72h. TPP, glucose, D-lactate, L-lactate, pH, pCO2, HCO3 -, BE, Na+, K+, Cl-, SID3, SIG, AG, Atot and percentage change in plasma volume (%PV) were measured or calculated and analyzed by two-way repeated measures ANOVA. All the calves developed osmotic diarrhea, mild to moderate dehydration, hyponatremia, relative hyperchloremia, and moderate to severe metabolic acidosis. The tested OESs were well accepted by the calves and effective in reversing dehydration, electrolyte imbalances, and metabolic acidosis. OES D did not completely correct hyponatremia, and SEO B caused transient hyperglycemia. It has been concluded that all the tested OESs are safe and effective for the treatment of diarrhea in calves with moderate degrees of dehydration and metabolic acidosis, which indicates that they have proper compositions for this purpose. trial (OESs water acidbase acid base spironolactone 4 10day 10 435 43 5 43. 380 3 80 3.8 n=6 n6 n 6 (n=6 commercial (commercial A C noncommercial non non-commercial UEL. UEL . UEL) L intakes 48h start induction, induction , induction) 24h -24h day, day) 8h 16h 72h TPP glucose Dlactate, Dlactate lactate, lactate D-lactate Llactate, Llactate L-lactate pH pCO2 pCO HCO - BE Na Na+ K K+ Cl, Cl Cl- SID3 SID SIG AG %PV PV (%PV twoway way ANOVA hyponatremia hyperchloremia acidosis imbalances hyperglycemia purpose 1 38 8 3. n= (n= (n

RESUMO Em um ensaio clínico controlado e aleatório, foram comparados os efeitos de cinco soluções eletrolíticas orais (SEOs) sobre os equilíbrios hídrico, eletrolítico e ácido-base de bezerros neonatos diarreicos. Diarreia osmótica e desidratação foram induzidas com sacarose no leite, espironolactona e hidroclorotiazida, por 48h, em 30 bezerros Holandeses com 10 dias de idade, 43,5 ± 3,8kg de peso, e alimentados com leite natural. Eles foram distribuídos em cinco grupos de tratamento (n=6) de acordo com a SEO administrada (comercial: SEO A, B, C, D; e não comercial: SEO UEL). No dia do tratamento, os bezerros receberam 6L de SEO em duas doses além da ingestão de leite. Amostras de sangue venoso foram coletadas em -48h (início da indução), -24h, 0h (início do dia de tratamento), 8h, 16h, 24h, 48h e 72h. PPT, glicose, lactatos L e D, pH, pCO2, HCO3 -, BE, Na+, K+, Cl-, SID3, SIG, AG, Atot e variação percentual no volume plasmático (%VP) foram mensurados ou calculados, e analisados por meio de ANOVA de duas vias de medidas repetidas. Todos os bezerros desenvolveram diarreia osmótica, desidratação leve a moderada, hiponatremia, hipercloremia relativa e acidose metabólica moderada a grave. As SEOs testadas foram bem aceitas pelos bezerros e eficazes para a reversão da desidratação, dos desequilíbrios eletrolíticos e da acidose metabólica. A SEO D não corrigiu completamente a hiponatremia, e a SEO B causou hiperglicemia transitória. Conclui-se que todas as SEOs são eficazes para o tratamento da diarreia em bezerros com graus moderados de desidratação e de acidose metabólica, o que indica que possuem composições adequadas para esse fim. aleatório (SEOs hídrico ácidobase ácido base diarreicos hidroclorotiazida h 3 1 idade 435 43 5 43, 38kg kg 8kg peso natural n=6 n6 n 6 (n=6 comercial (comercial C UEL. UEL . UEL) início indução, indução , indução) 24h -24h tratamento) 8h 16h 72h PPT glicose pH pCO2 pCO HCO - BE Na Na+ K K+ Cl, Cl Cl- SID3 SID SIG AG %VP VP (%VP calculados repetidas hiponatremia grave transitória Concluise Conclui se fim 4 n= (n= (n

Resumen La identificación y evaluación del riesgo de explosividad e incendio inicia con la información de la infraestructura y áreas del Campamento del GAD Provincia de Pichincha, realizando la identificación del factor del riesgo y de los procesos en la estación de servicio del campamento, con ello se procede a una evaluación mediante la aplicación de 2 metodologías: NFPA y DOW. La evaluación de riesgo mediante NFPA determinó que la estación de servicio representa un nivel de repercusión alto no tolerable para el Campamento; la evaluación de riesgo mediante DOW determinó la existencia de un índice de riesgo de explosividad e incendio de categoría importante, toda vez que el área 1 es un área mixta en el que se localizan 2 surtidores de abastecimiento de combustible y 2 bocatomas para el trasvase de combustible a los tanques de abastecimiento. Adicionalmente dentro del área de influencia de la E/S se evidencia un importante escenario socio-ambiental, por la presencia del río Blanco, cobertura vegetal y propiedad privada colindante, en caso de generarse un accidente mayor, dado por el índice de riesgo de incendio-explosión que produciría derrame de combustible, con el conocimiento del radio y área de exposición, se generaría un efecto sinérgico en el entorno por lo que se procedió a realizar una evaluación basada en metodología Leopold del cual se obtuvo como resultado un impacto ambiental de nivel crítico para los recursos suelo y agua (tramo del río Blanco) así como para la fauna y el paisajismo, de nivel severo para el recurso flora y propiedad colindante norte y finalmente de nivel moderado para la calidad del aire y la ornitofauna.

Abstract The identification and evaluation of the risk of explosiveness and fire starts with the information of the infrastructure and areas of the Camp of the GAD Province of Pichincha, identifying the risk factor and the processes at the camp service station, with this, an evaluation is carried out through the application of 2 methodologies: NFPA and DOW. The risk assessment determined by NFPA that the service station represents a high level of repercussion not tolerable for the Camp; the risk assessment by DOW determined the existence of an explosiveness and fire risk index of category important, since area 1 is a mixed area in which 2 fuel supply pumps are located and 2 intakes for the transfer of fuel to the tanks of catering. Additionally within the area of influence of the E/S an important socio-environmental scenario is evidenced, by the presence of the Blanco River, vegetation cover and property adjoining private, in the event of a major accident, given by the fire-explosion risk index that would produce fuel spill, with knowledge of the radius and area of exposure, a synergistic effect would be generated in the environment Therefore, an evaluation was carried out based on Leopold methodology which resulted in a critical level environmental impact for soil and water resources (section of the Blanco River) as well as for fauna and landscaping, of severe level for the flora resource and adjoining property north and finally moderate level for air quality and ornithofauna.

Abstract Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight. It is unknown if these consumption patterns are related to body composition. This research aimed to determine the relationship between pattern of food consumption and body composition in university students. Method: an observational, descriptive, cross-sectional study with a sample of 189 students. Sociodemographic variables obtained by a survey, body composition variables obtained by bioimpedance, and food groups and their categories obtained via a 24-hour reminder method were included. A multinomial logistic regression model was performed to explain body composition according to consumption of food groups. Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013). The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55). Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14). The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60). Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass. The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water. Studies to establish associations between body composition and food consumption should be carried out taking into account specific intakes in order to establish a more accurate association.

Resumen Introducción: los jóvenes universitarios enfrentan diversas situaciones sociales que generan cambios en los estilos de vida. Estos están asociados a desequilibrios en la ingesta dietética, caracterizada por una gran densidad calórica, lo que produce sobrepeso. Al mismo tiempo, se conoce la relación entre los patrones de consumo y la composición corporal, pero existe un vacío en la población universitaria colombiana. Así pues, el objetivo de esta investigación fue determinar la relación del patrón de consumo de alimentos con la composición corporal en universitarios. Método: estudio observacional, descriptivo, de corte transversal con una muestra de 189 estudiantes. Se incluyeron variables sociodemográficas obtenidas por encuesta, variables de composición corporal derivadas de la bioimpedancia, y los grupos de alimentos y sus categorías por el método del recordatorio de 24 horas. Se realizó un modelo de regresión logística multinomial para explicar la composición corporal de acuerdo con el consumo de los grupos de alimentos. Resultados: se obtuvieron asociaciones estadísticamente significativas entre el consumo de alimentos y la composición corporal para la grasa visceral y el consumo de huevos, carnes, embutidos y granos (p = 0,024), y para el consumo de azúcares con respecto a la masa ósea (p = 0,029) y la masa muscular (p = 0,013). El modelo de regresión múltiple mostró que el consumo de azúcar incrementa la probabilidad de tener grasa visceral (OR = 2,71) y la probabilidad de clasificar a una persona como obesa (OR = 2,55). El consumo de huevos, carnes, embutidos y granos favorece la clasificación de la grasa visceral como aceptable (OR = 0,14). El consumo de frutas y verduras incrementa la probabilidad de presentar un alto porcentaje de agua (OR = 5,41) y la clasificación del porcentaje de grasa como bajo (OR = 3,60). Conclusiones: el consumo de frutas y verduras se corresponde con un porcentaje bajo de grasa; no obstante, su ingesta se vincula proporcionalmente con altos niveles de agua corporal. Se deben realizar estudios que establezcan asociaciones entre la composición corporal y el consumo de alimentos, teniendo en cuenta la ingesta específica que pueda establecer una correlación con mayor precisión.

Abstract Background: icons or shapes represent the development and approval of dietary guidelines including recommendations of food consumption and nutrient intakes according to the behavioral models of countries worldwide. The Food and Agriculture Organization (FAO) collected and recognized them. Methods: food-based dietary guidelines from 94 countries included in the FAO website. Websites of the different Spanish scientific societies that have elaborated and developed them, including the Mediterranean Diet Foundation. Results: in all, 94 countries are collected in the FAO food guidelines website, with predominant models of 33 diet plates and 51 food pyramids, emerging 11 as new graphics systems, that reflect, in addition to food: 52, physical activity; 63, water or hydration; 20, social and emotional values; and 13, reduction or elimination of alcoholic beverages. Conclusions: the models with icons or shapes so far are consistent with the modes of distribution of food at meals and adapting to new criteria that include behavioral guidelines. New designs are incorporated that include food safety guidelines in generic graphic structures that do not represent any of the classic models: food plates or food pyramids.

Resumen Fundamentos: el desarrollo y la aprobación de guías alimentarias, con recomendación de consumo de alimentos y de ingestas de nutrientes según los distintos modelos comportamentales de los países a nivel mundial, culmina con la creación de iconos o grafismos que las representan. Estos, además, son recogidos y reconocidos por la Organización para la Alimentación y la Agricultura (FAO). Métodos: guías alimentarias de 94 países incluidas en la web de la FAO y de las diferentes sociedades científicas españolas que las tienen elaboradas y desarrolladas, así como la de la Fundación Dieta Mediterránea. Resultados: en la web de la FAO se recogen 94 países con modelos predominantes de 33 platos y 51 pirámides, surgiendo 11 casos de sistema nuevo que plasman, además de alimentos: 52, actividad física; 63, agua o hidratación; 20, aspectos sociales y emocionales, y 13, reducción o eliminación de bebidas alcohólicas. Conclusiones: los modelos de iconos o grafismos hasta ahora son parejos con los modos de distribución de alimentos en las comidas, adaptándose a los nuevos criterios que incluyen pautas comportamentales. Se incorporan nuevos diseños que incluyen pautas de seguridad alimentaria en estructuras gráficas genéricas que no representan ninguno de los modelos clásicos (platos o pirámides alimentarios).

This study was designed to evaluate the nutrient intake, digestibility, degradability, and ruminal characteristics of Sindhi heifers fed diets that contained a combined total of 75% oldman saltbush hay (hereafter saltbush hay) and prickly pear cactus. Eight 12-month old intact male red Sindhi calves (four fistulated and four non-fistulated) with an initial mean weight of 170 ± 5 kg were assigned to 4 * 4 Latin squares, where factors consisted of four diets, namely 15% hay and 60% cactus; 30% hay and 45% cactus; 45% hay and 30% cactus; and 60% hay and 15% cactus, and four times at which rumen fluid was collected. Neutral detergent fibre (NDF) and acid detergent fibre (ADF) intakes in kg/day and NDF in percentage live weight (LW), water intake, salinity, and conductivity increased with hay level. Intake and digestibility of non-fibrous carbohydrates were curvilinear with higher values in diets containing 30% saltbush hay. The apparent digestibility of dry matter (DM) and organic matter decreased linearly as the concentration of saltbush hay increased in the diet. The pH of the rumen fluid was within the acceptable range for favourable microbial growth. The low temperature and high salinity and conductivity indices in the diets should be viewed with caution at higher concentrations of saltbush hay, because of a possible decrease in nutrient absorption and the development of health problems in the animals. Apparent degradability coefficients of DM and NDF were affected significantly by inclusion of prickly pear and saltbush hay in the diets.

RESUMO Objetivo Determinar o efeito de hábitos nutricionais na formação e recorrência de cálculos renais. Métodos Este estudo foi realizado em 44 indivíduos saudáveis e 44 pacientes com diagnóstico de nefrolitíase e envelhecimento entre 20 e 65 anos. Os participantes compartilharam seus hábitos de consumo de sal, quantidades diárias de consumo de líquidos e informações gerais sobre si mesmos em um questionário. Além disso, as frequências de consumo de alimentos e bebidas dos participantes foram registradas por meio de um questionário de frequência alimentar. Resultados As frequências de consumo de sal dos pacientes são maiores que as de indivíduos saudáveis em ambos os sexos (p<0,05). Verificou-se que os indivíduos do sexo masculino no grupo de pacientes salgam os pratos sem degustar com maior frequência (p<0,05). O consumo diário total de água de ambos os sexos no grupo de pacientes é menor que o dos indivíduos saudáveis (p<0,05). O consumo de carne de pacientes do sexo masculino (51,6±31,35g/dia) foi maior que o do grupo saudável (34,1±22,58g/dia) (p<0,05). Além disso, indivíduos do grupo de pacientes consomem menos urtiga, milho, ameixa, nêspera, suco de laranja e limonada do que indivíduos saudáveis (p<0,05). Conclusão Os resultados do estudo mostraram que a ingestão total de líquidos, os hábitos de consumo de sal e o consumo de vegetais, frutas e bebidas podem estar correlacionados com o risco de formação de pedra e os hábitos de nutrição podem afetar a recorrência da pedra.

ABSTRACT Objective To determine the effect of nutritional habits on kidney stone formation and recurrence. Methods This study was conducted on 44 healthy individuals and 44 patients diagnosed with nephrolithiasis and aging between 20 and 65 years. Participants shared their salt consumption habits, daily fluid consumption amounts and general information about themselves in a questionnaire form. In addition, food and beverage consumption frequencies of participants were recorded through a food frequency questionnaire. Results Salt consumption frequencies of patients are higher than that of healthy individuals in both genders (p<0.05). It was found out that male individuals in the patient group salt dishes without tasting more frequently (p<0.05). Daily total water consumption of both genders in patient group is lower than that of healthy individuals (p<0.05). Meat consumption of male patients (51.6±31.35g/day) was found to be higher than that of healthy group (34.1±22.58g/day) (p<0.05). Additionally, individuals in the patient group consume less stinging nettle, corn, plum, loquat, orange juice and lemonade than healthy individuals (p<0.05). Conclusion Results of the study showed that total fluid intake, salt consumption habits, and vegetable, fruit and beverage consumption may be correlated with stone formation risk and nutrition habits may affect stone recurrence.

Nutrition contributes approximately 75% of the total input costs to an intensive ostrich production unit. An increase in the price of traditional protein sources thus necessitates finding cheaper alternatives. Sweet lupins were identified as a possible alternative; therefore, the effect of feeding various sweet lupin inclusion levels was evaluated throughout the different feeding phases. One hundred and forty ostrich chicks were randomly divided into five dietary treatments with three replications each. The chicks all received a standard commercial pre-starter ostrich diet. Five iso-nutritional diets were formulated for each feeding phase according to specifications set out for each of the different feeding phases using Mixit2+ software. For each feeding phase the diets contained either soybean oilcake meal (control diet, 0LD) or sweet lupins (alternative protein source). The 100 lupin diet (LD) was formulated to include the maximum amount of sweet lupins according to the specifications for the specific species and the feeding phase, and therefore differs between the three feeding phases. Soybean oilcake meal was thus gradually replaced by sweet lupins in the following ratios: 100:0 (0LD), 75:25 (25LD), 50:50 (50LD), 25:75 (75LD), and 0:100 (100LD). Feed and water were supplied ad libitum. Feed intakes and live weights were measured every fortnight until slaughter at ca. 11 months of age. No differences were found between the treatment diets for the live weight, dry matter intake (DMI), average daily gain (ADG) or feed conversion ratio (FCR) at the end of each feeding phase. However, the birds on the 75LD tended to have the highest end weight and ADG, and those on the 100LD the lowest at the end of the starter phase. No differences were found for slaughter weight, dressing percentage and big drum muscle weight of the birds. Birds on the 50LD and 75LD tended to have the heaviest cold carcasses; this differed from the 100LD birds which had the lightest. Birds fed the 50LD had heavier thigh weights than those on the other diets. The results of this study indicate that soybean oilcake meal can be replaced in the diets of slaughter ostriches with sweet lupins up to 15% (75LD) in starter diets and 30% (100LD) in grower and finisher diets without any significant effect on any of the production and slaughter traits.

Objectives of the experiment were to determine effects of dietary hay concentration in a dry total mixed ration and its carryover effects on intake, growth performance, faecal score, and feed efficiency of weaned dairy calves. Eighteen Friesian χ Jersey weaned calves (n = 6 calves/treatment) were randomly assigned to three rhodes grass hay treatments (RG13, RG26, and RG39). The experimental diets were rhodes grass hay-based total mixed rations containing 13%, 26%, and 39% chopped hay on a DM basis. The experiment had two phases of four weeks each. In phase 1 (weeks 1-4), weaned calves were fed RG13, RG26, or RG39. Then, in phase 2 (weeks 5-8), all calves were shifted to a maize silage-based diet. All the diets were iso-nitrogenous and were fed ad libitum. Calves were housed in individual pens and had free access to water and feed. Average daily gain and daily dry matter intake were analysed as repeated measures, whereas bodyweight and feed efficiency were analysed using one-way ANOVA. In phases 1 and 2 dry matter intakes were similar. Growth rate decreased linearly with increasing concentration of hay in phase 1. Overall, daily dry matter intake, average daily gain, change in body condition score and structural measurements were not affected by dietary treatments. However, overall feed efficiency was improved for calves fed RG26 compared with RG13 and RG39. Thus, feeding a moderate level of hay had positive impacts on the transition to a silage-based TMR.

Abstract Introduction: the dietary water and the intake of beverages play a relevant role in the dietary and hydration patterns of children and few studies provide an overview of them. Objetives: to assess the dietary intake of water, its adaptation to the recommendations and its distribution throughout the day, and the average consumption of beverages by a group of Spanish schoolchildren. Material and methods: a sample of 262 schoolchildren (139 boys and 123 girls) between seven and 12 years old was studied. A three-day dietary record (one day in weekend) was kept to assess beverage consumption. Dietary water intake obtained was compared to EFSA's adequate intake (AI) and other recommendations, its distribution throughout the day was studied, and personal and anthropometric date were collected too. The data were processed using DIAL and SPSS. Results: the beverages consumed in greatest quantity were water as a beverage, milk and commercial juices and nectars. The average dietary water intake was 1,401 ± 432 ml/day (42% from food and 58% from beverages). Only 20.2% of schoolchildren were above the AIs. Dietary water intake was significantly higher at all intakes in the day in those schoolchildren who were above AIs. Conclusions: based on our results, 79.8% of children do not cover the AIs. For this reason it is important improve their hydration patterns. In this aspect, water as a beverage plays a key role to cover the recommendations.

Resumen Introducción: la ingesta dietética de agua y el consumo de bebidas juegan un papel relevante en los hábitos de hidratación y alimentación de la población infantil y escasos estudios nos ofrecen una visión global de los mismos. Objetivos: evaluar la ingesta dietética de agua y el consumo de bebidas, su adecuación a las recomendaciones y su distribución a lo largo del día en un colectivo de escolares españoles. Material y métodos: se estudió una muestra de 262 escolares (123 niñas) entre siete y 12 años. Se realizó un registro dietético de tres días (uno de ellos festivo) para evaluar el consumo de bebidas. Se comparó la ingesta dietética de agua obtenida con las ingestas adecuadas (IA) marcadas por la EFSA y por otros organismos y se estudió su distribución a lo largo del día. También se procedió a recoger datos personales y antropométricos. Los datos fueron procesados mediante DIAL y SPSS. Resultados: las bebidas más consumidas fueron agua de bebida, leche y zumos comerciales y néctares. La ingesta dietética media de agua fue de 1.401 ± 432 ml/día (42% aportada por alimentos y 58% por bebidas). Solo el 20,2% de los escolares superaron las IA. La ingesta dietética de agua fue significativamente superior en todos los momentos del día en aquellos escolares que superaron las IA establecidas. Conclusión: el 79,8% de los escolares no alcanza las IA, por lo que es importante mejorar sus pautas de hidratación, jugando el agua de bebida un papel clave a la hora de alcanzar las recomendaciones.

Abstract Objective To estimate fluoride intake through consumption of water from the municipal network in pregnant women and their children from the INMA-Gipuzkoa cohort and to compare these intakes with recommended levels. In Euskadi (Spain), fluoridation of drinking water is compulsory in water supplies for more than 30,000 inhabitants. Method 575 pregnant women (recruitment, 2006-2008) and 424 4-year-old children (follow-up, 2010-2012) have been included. Fluoride levels in drinking water were obtained from the water consumption information system of the Basque Country (EKUIS). Water consumption habits and socioeconomic variables were obtained by questionnaire. Results 74.9% and 87.7% of women and children consumed water from the municipal network. Average fluoride levels in fluoridated water were 0.805 (SD: 0.194) mg/L during baseline recruitment and 0.843 (SD: 0.080) mg/L during follow up, at 4 years old of the children. Average and 95th percentile of fluoride intake were 0.015 and 0.026mg/kg per day in women and 0.033 and 0.059mg/kg per day in children. Considering only fluoride provided by drinking water, 8.71% of children living in fluoridated areas exceeded intake level recommended by the European Food Safety Authority, consisting in 0.05mg/kg per day. Conclusion The results show that ingested levels of fluoride through consumption of municipal water can exceed the recommended levels in children and encourages further studies that will help in fluoridation policies of drinking water in the future.

Resumen Objetivo Estimar la ingesta de flúor en mujeres embarazadas y sus hijos/as de la cohorte INMA-Gipuzkoa a través del consumo de agua de la red municipal y comparar estas ingestas con los valores recomendados. En Euskadi, la fluoración del agua de consumo es obligatoria en abastecimientos de más de 30.000 habitantes. Método Se han incluido 575 mujeres embarazadas (reclutamiento en 2006-2008) y 424 niños/as de 4 años (seguimiento en 2010-2012). Las concentraciones de fluoruros en el agua se obtuvieron del sistema de información de aguas de consumo de Euskadi (EKUIS). Los hábitos de consumo de agua y las variables socioeconómicas se obtuvieron mediante cuestionario. Resultados El 74,9% de las mujeres y el 87,7% de los/las niños/as consumían agua de red municipal. En agua fluorada, el valor medio de fluoruro fue de 0,805mg/l (desviación estándar [DE]: 0,194) durante el periodo de reclutamiento, y de 0,843mg/l (DE: 0,080) durante el seguimiento de los/las niños/as. La ingesta media de flúor y el percentil 95 en las zonas fluoradas fueron de 0,015 y 0,026mg/kg al día en las mujeres y de 0,033 y 0,059mg/kg al día en los/las niños/as. Considerando solo el flúor aportado por el agua, el 8,71% de los/las niños/as residentes en zonas con fluoración superaban la ingesta de 0,05mg/kg al día recomendada por la European Food Safety Authority. Conclusión Los resultados obtenidos muestran que las ingestas de flúor a través del agua de consumo pueden superar lo recomendado en población infantil, y propician futuros estudios que aporten evidencias que puedan ayudar en las políticas de fluoración de las aguas de consumo público.

Abstract Objective To estimate fluoride intake through consumption of water from the municipal network in pregnant women and their children from the INMA-Gipuzkoa cohort and to compare these intakes with recommended levels. In Euskadi (Spain), fluoridation of drinking water is compulsory in water supplies for more than 30,000 inhabitants. Method 575 pregnant women (recruitment, 2006-2008) and 424 4-year-old children (follow-up, 2010-2012) have been included. Fluoride levels in drinking water were obtained from the water consumption information system of the Basque Country (EKUIS). Water consumption habits and socioeconomic variables were obtained by questionnaire. Results 74.9% and 87.7% of women and children consumed water from the municipal network. Average fluoride levels in fluoridated water were 0.805 (SD: 0.194) mg/L during baseline recruitment and 0.843 (SD: 0.080) mg/L during follow up, at 4 years old of the children. Average and 95th percentile of fluoride intake were 0.015 and 0.026mg/kg per day in women and 0.033 and 0.059mg/kg per day in children. Considering only fluoride provided by drinking water, 8.71% of children living in fluoridated areas exceeded intake level recommended by the European Food Safety Authority, consisting in 0.05mg/kg per day. Conclusion The results show that ingested levels of fluoride through consumption of municipal water can exceed the recommended levels in children and encourages further studies that will help in fluoridation policies of drinking water in the future.

Resumen Objetivo Estimar la ingesta de flúor en mujeres embarazadas y sus hijos/as de la cohorte INMA-Gipuzkoa a través del consumo de agua de la red municipal y comparar estas ingestas con los valores recomendados. En Euskadi, la fluoración del agua de consumo es obligatoria en abastecimientos de más de 30.000 habitantes. Método Se han incluido 575 mujeres embarazadas (reclutamiento en 2006-2008) y 424 niños/as de 4 años (seguimiento en 2010-2012). Las concentraciones de fluoruros en el agua se obtuvieron del sistema de información de aguas de consumo de Euskadi (EKUIS). Los hábitos de consumo de agua y las variables socioeconómicas se obtuvieron mediante cuestionario. Resultados El 74,9% de las mujeres y el 87,7% de los/las niños/as consumían agua de red municipal. En agua fluorada, el valor medio de fluoruro fue de 0,805mg/l (desviación estándar [DE]: 0,194) durante el periodo de reclutamiento, y de 0,843mg/l (DE: 0,080) durante el seguimiento de los/las niños/as. La ingesta media de flúor y el percentil 95 en las zonas fluoradas fueron de 0,015 y 0,026mg/kg al día en las mujeres y de 0,033 y 0,059mg/kg al día en los/las niños/as. Considerando solo el flúor aportado por el agua, el 8,71% de los/las niños/as residentes en zonas con fluoración superaban la ingesta de 0,05mg/kg al día recomendada por la European Food Safety Authority. Conclusión Los resultados obtenidos muestran que las ingestas de flúor a través del agua de consumo pueden superar lo recomendado en población infantil, y propician futuros estudios que aporten evidencias que puedan ayudar en las políticas de fluoración de las aguas de consumo público.