Abstract Introduction: Urban development and expansion have led to the loss and transformation of Andean ecosystems. However, remnant forests that maintain biodiversity still exist around Colombia’s major cities, but these forests are threatened by the road infrastructure. Road ecology in the Neotropics has been focused specifically in documenting the effects of roadkill from a taxonomic point of view, mainly in rural areas. Objective: To evaluate ecologically and spatially, the collision of fauna on roads located in areas of urban expansion in the Northern Colombian Andes. Methods: We assessed vertebrate mortality rate and identified the critical areas of roadkill by taxonomic and functional groups. This was achieved through spatial ecology analysis, specifically Kernel density analysis and hotspot spatial statistical analysis. Additionally, we identified landscape features associated with road mortality and assessed the role of roads as resistance and permeability factors. Results: A total of 279 roadkilled animals belonging to 64 species were found, accounting for 28 % of the 273 vertebrate species recorded in the reserve near the studied roads. The average mortality rate for vertebrates was estimated from 42 to 170 individuals annually. Differential patterns were detected in roadkill density across taxonomic and functional groups, where nocturnal species and the trophic guilds are the most vulnerable. Likewise, it was found that the sections of the roads close to the forest have a greater roadkill species richness with greater ecological requirements. These sections constitute an important resistance to species movement in the area. Conclusions: Road mortality in the study area exhibits heterogeneous spatial patterns not only at a taxonomic but also at a functional level, suggesting the deterioration of protected ecosystems in adjacent peri-urban areas.

Resumen Introducción: El desarrollo y expansión urbana han generado la pérdida y transformación de los ecosistemas andinos. Sin embargo, alrededor de las grandes ciudades colombianas aún se encuentran relictos boscosos que mantienen gran riqueza de biodiversidad, la cual es amenazada por la infraestructura vial. El énfasis de los trabajos de Ecología de Carreteras en el Neotrópico ha estado centrado en el efecto del atropellamiento de fauna desde el punto de vista taxonómico. Objetivo: Evaluar, ecológica y espacialmente, el atropellamiento de la fauna en carreteras ubicadas en zonas de interés para la expansión urbana del norte de los Andes colombianos. Métodos: Se evaluaron la tasa de mortalidad de vertebrados, las zonas críticas de atropellamientos por grupo taxonómico y grupos funcionales. Esto se realizó por medio de análisis de ecología espacial, específicamente análisis de densidad de Kernel y análisis de estadística espacial de puntos calientes. Adicionalmente, se identificó las características del paisaje asociadas al atropellamiento y se evaluó el papel de las vías como factor de resistencia y permeabilidad. Resultados: Se encontraron 279 animales atropellados de 64 especies que representan el 28 % de las 273 especies vertebradas presentes en las áreas protegidas en inmediaciones de las carreteras estudiadas. Se estimó una tasa promedio entre 42 y 170 individuos atropellados por año. Se detectaron patrones diferenciales en la densidad de atropellamiento por grupos taxonómicos y funcionales, donde las especies nocturnas y el tipo de gremio trófico son los más vulnerables. Asimismo, se encontró que los tramos de las vías en cercanía a coberturas boscosas presentan mayor riqueza de especies atropelladas y con mayores requerimientos ecológicos. Estos tramos representan una resistencia importante al movimiento de las especies de la zona. Conclusiones: El atropellamiento presenta patrones espaciales heterogéneos, no solo a nivel taxonómico, sino también a nivel funcional, lo cual representa un deterioro para los ecosistemas protegidos en zonas periurbanas.

ABSTRACT Penaeus vannamei, or white shrimp, is an economically significant species that dominates shrimp production and represents a rapidly growing industry. However, the culture faces challenges due to high mortality rates due to bacterial and viral diseases. Unlike vertebrates, shrimp rely solely on their innate immunity for defense against pathogens. Introducing probiotics into shrimp culture can enhance growth, survival rates, and immune responses against disease outbreaks caused by pathogenic bacteria or viruses. Common diseases affecting shrimp farms include vibriosis, white spot disease, acute hepatopancreatic necrosis and black gill disease. These diseases can cause severe infections, which lead to high mortality rates. The application of probiotics, such as Bacillus cereus, B. licheniformis, B. subtilis, Lactobacillus sp., Paenibacillus, Pseudomonas sp., Pseudoaltermonas, and yeast, either through diet or direct administration, has demonstrated positive impacts on shrimp health, including enhanced growth, increased immunostimulation, improved gut microbiota, and better water quality parameters. In recent decades, aquafeed has explored effective solutions, such as microdiets, co-feeding (combining commercial feed and probiotics), and various microdiets types, to address aquaculture disease problems. Combining microdiets containing essential nutrients with probiotics has proven to be safer than antibiotics, which can adversely affect animals and the environment. However, further studies are needed to optimize the efficiency of feeding techniques by incorporating probiotics and commercial feed for disease resistance in penaeid shrimp culture. This review discusses commercial feeds and microdiets incorporated with probiotics for P. vannamei culture for growth and disease resistance to enhancement.

The number of people on Earth and human activities are currently taking on frightening proportions with far-reaching consequences for our planet, its biodiversity and ultimately the human race itself. Ten thousand years ago, humans represented 1% of the total biomass of all land vertebrates. The rest was made up by wild animals. Currently, humans, livestock and pets make up 99% of terrestrial vertebrate biomass, while wild animals represent only 1%. It took millennia for the human population to reach the 1 billion mark and that only by 1890. By 1960, however, the world population had grown to 3 billion. Today there are 8 billion people living on our planet. According to Population Matters, the world population will be approximately 10,4 billion by the end of this century, despite the gradual decline in the growth rate in some countries. There is only a 23% chance that the total population growth rate will tail off before 2100. The population of the 47 least developed countries is likely to triple between 2017 and 2100 to around 3 billion. There are currently 62 million people in South Africa. About 67,9% of our population (in 2021) live in urban areas. The South African population grew by 0,87% in 2023. The fertility rate is 2,30 (2023). It is estimated that by 2050 75,5 million people will be living in South Africa, with 8 out of every 10 people living in urban areas, which would significantly increase the pressure on infrastructure. The Global Footprint Network states that humans currently use the resources of 1,75 Earths. If the situation does not improve, by 2050 we would need the equivalent of three Earths to survive. More than 4 billion people will have little or no water by 2050, and South Africa even now has been classified as a "water-stressed" country. Medical science is continually contributing to more babies surviving their entry into the world and to our living increasingly longer. Life expectancy has risen from 46 years in 1950 to 73,2 years in 2023. By 2050 it will be 77 years. What can be done? Empowerment of women is important. Population growth can be curbed to a reasonable extent by giving more girls access to education. This certainly does not mean that girls or women alone should be held responsible for the current population growth, but much more will have to be done about sex education, women's rights will have to be actively promoted and there will have to be better provision and use of contraceptives. Obviously, these issues should be approached and handled with due caution. In this regard, countries such as Thailand and South Korea have, in the recent past, proved that good education may result in lower birth rates. In Africa, women with no education have an average of 5,4 children; women who completed high school an average of 2,7 children and women with tertiary education an average of 2.2. In Nigeria, every year of school attendance by girls cuts the birth rate by a tenth. In addition, intensified efforts to reform economies and societies should be considered. For the sake of the survival of the human species and the sustainability of the Earth, ecological and economic justice and the empowerment ofwomen have to be encouraged. The Millennium Project - a global participatory think tank that was founded in 1996 under the auspices of the American Council for the United Nations University, but which became independent in 2009 and has since grown into a global network of institutions and individuals - aims to improve humankind's prospects for a better future. It recommends, among other things, the following: • Support a policy aimed at improving the survival of children, responsible family planning and educational opportunities for (especially) girls. • Improve methods that will ensure skills and employment opportunities for young people, as well as care and services for the elderly. • Implement the UN's urban agenda. • Create enough smart cities where citizens with urban intelligence can help in eco-smart ways with the necessary improvements in the city. • Increase training in resilience, and improve disaster prediction and management. • Provide guidance for people about urban ecology. • Intensify research and development in saltwater agriculture (halophytes or salt plants) on coastlines to produce food for humans and animals, biofuel and pulp for the paper industry, and to absorb carbon dioxide (CO2). This will ease the pressure on freshwater agriculture. • Improve agricultural and irrigation management. • Invest in precision agriculture and aquaculture. • Reduce food losses from the "farm to the mouth" (one third or 1,3 billion tons of agricultural production is wasted in this way every year). • Establish seaweed to restore wild fish populations along coastlines. The World Bank estimates that 90% of marine life is completely depleted. • Expand the production of insects for animal feed and human diets (insects have a low environmental impact, and 2 billion people already supplement their diet with insects today). • Encourage vegetarianism. Although there are more ways to address these important global challenges, responsible family planning remains one of the most important. This is an extremely sensitive issue for many people (especially in Africa), but if it is not promoted in a humane, informed and responsible way, the sustainability of life on our planet will increasingly come under tremendous pressure in various respects.

Die aantal mense op aarde en hulle/ons aktiwiteite neem tans skrikwekkende afmetings aan met wydlopende gevolge vir ons planeet, biodiversiteit en uiteindelik onsself. Die moderne mens het sy teenwoordigheid op die aarde en die benutting van hulpbronne baie lank as sy onaantasbare reg gesien. Die huidige uitwerking van byvoorbeeld bevolkingsgroei en besoedeling versteur die aarde se natuurlike ritme in so 'n mate dat ons nou nie meer op 'n kitsoplossing kan hoop nie. Die mens se getalle neem teen so 'n tempo toe dat dit nie net nadelig vir die planeet is nie, maar ook vir die mensdom self. Wat kan gedoen word? Bemagtiging van vroue speel 'n belangrike rol. Dit beteken beslis nie dat meisies en vroue alleen vir die huidige bevolkingsgroei verantwoordelik gehou moet word nie. Die bevolkingsgroei kan egter in 'n redelike mate vertraag word deur veral meer meisies toegang tot onderwys en opleiding te gee - asook hul lewensomstandighede te verbeter. Ook sal daar heelwat meer aan seksopvoeding gedoen moet word, vroueregte sal daadwerklik bevorder moet word en daar sal beter voorsiening en gebruik van voorbehoedmiddels moet wees. Uiteraard moet hierdie kwessies met die nodige omsigtigheid benader en hanteer word. In hierdie verband kan onder andere Thailand en Suid-Korea genoem word wat in die onlangse verlede bewys het dat goeie onderwys geboortesyfers laat daal. In Afrika het vroue wat geen onderwys ontvang nie, gemiddeld 5,4 kinders; vroue wat hoërskool voltooi het gemiddeld 2,7 kinders en vroue wat tersiêre studie deurloop het, gemiddeld 2,2 kinders. In Nigerië sny elke jaar wat meisies skool bywoon, die geboortesyfer met 'n tiende. Hiermee saam sal daar ook verskerpte pogings moet wees om ekonomieë en samelewings te hervorm. Ter wille van die mens se voortbestaan en die volhoubaarheid van lewe op aarde moet ekologiese en ekonomiese geregtigheid, en die bemagtiging van vroue aangemoedig word.

Abstract Introduction: Even though only a few species are considered to be dangerous, pests or vectors, the majority of invertebrates produce a feeling of aversion in humans. This has contributed to the delay in the development of ethical considerations as regards this group in contrast with vertebrates, with the exception of cephalopods. Objective: In the present study, we provide an overview of the current situation on animal ethics and welfare in order to contribute to the development of a framework for ensuring invertebrate welfare. Methods: Today, animal welfare is multidisciplinary in nature to a very high degree as it includes ethology, physiology, pathology, biochemistry, genetics, immunology, nutrition, cognitive-neural, veterinary medicine, and ethics. Animal welfare is a complex concept, difficult to achieve successfully from one perspective. Results: As a consequence, we propose to include the five domains (nutrition, environment, health, behaviour and mental state) along with the three conceptions (basic health and functioning, affective state and natural living), as well as the 5R Principle (Replace, Reduction, Refinement, Respect and Responsibility) in seeking to achieve a comprehensive welfare state. Conclusions: We consider that in both research and animal production, the individual and collective ethical concerns coexist and, in fact, the main moral concern to account for is the collective one and that, within that collective view, the individual moral concern should be applied with responsibility and respect for the individual. Finally, we propose a practical example of invertebrate welfare production in sea urchin aquaculture with the aim of including animal production of invertebrates in this important discussion.

Resumen Introducción: Aunque sólo unas pocas especies son consideradas peligrosas, plagas o vectores, la mayoría de los invertebrados producen un sentimiento de aversión en el ser humano. Esto ha contribuido al retraso en el desarrollo de consideraciones éticas respecto a este grupo en comparación con los vertebrados, a excepción de los cefalópodos. Objetivo: En el presente trabajo, proporcionamos una visión general de la situación actual en materia de ética y bienestar animal con el fin de contribuir al desarrollo de un marco para garantizar el bienestar de los invertebrados. Métodos: Hoy en día, el bienestar animal es de naturaleza multidisciplinaria en un grado muy alto, ya que incluye etología, fisiología, patología, bioquímica, genética, inmunología, nutrición, cognitivo-neural, medicina veterinaria y ética. El bienestar animal es un concepto complejo, difícil de lograr con éxito desde una sola perspectiva. Resultados: Como consecuencia, proponemos incluir los cinco dominios (nutrición, ambiente, salud, comportamiento y estado mental) junto con las tres concepciones (Salud básica y funcionamiento, estado afectivo y vida natural), así como el Principio 5R (Reemplazar, Reducir, Refinar, Respetar y Responsabilidad) en la búsqueda de alcanzar un estado de bienestar integral. Conclusiones: Consideramos que tanto en la investigación como en la producción animal coexisten las preocupaciones éticas individuales y colectivas y, de hecho, la principal preocupación moral a dar cuenta es la colectiva y que, dentro de esa visión colectiva, se debe aplicar la preocupación moral individual. con responsabilidad y respeto por la persona. Finalmente, proponemos un ejemplo práctico de producción de bienestar de invertebrados en la acuicultura de erizos de mar con el objetivo de incluir la producción animal de invertebrados en esta importante discusión.

Abstract Sustainable forest management requires the integration of better practices for the conservation of biological diversity. Therefore, it is necessary to know the composition and richness of wild communities in sites where different forest management practices are applied. To this end, the fauna of managed forests in the Chignahuapan region of Puebla State was sampled for four years. A total of 112 species were recorded: 13 medium and large mammals, 83 birds, four amphibians and 12 reptiles. The abundances of these observed species were used to calculate the expected richness with the non-parametric Chao1 estimator. The management method with the highest expected richness was the Silvicultural Development Method (MDS) with 113, followed by Successive Cutting (CS) with 97, and the lowest was observed in the Mexican Method for the Management of Irregular Forests (MMOBI) with 71. Sampling completeness ranged from 92 % in amphibians to 99 % in mammals. Sixteen species are in some risk category in the official Mexican standard NOM-059-SEMARNAT-2010, and 25 are endemic to Mexico. Taxonomic lists contain information on conservation status, endemism and relative abundance. Intensive forest management methods, such as MDS, have a richer mammal and bird community than MMOBI and CS but are similar to protected areas. The richness and abundance of amphibians and reptiles, most of them in some risk category, are low, especially in MMOBI and CS, and almost absent in protected areas.

Resumen El manejo forestal sustentable requiere de la integración de mejores prácticas de conservación de la diversidad biológica. Para esto, es necesario conocer la composición y riqueza de las comunidades silvestres en los sitios donde se aplican diferentes prácticas silvícolas. Con esta finalidad, se muestreó durante cuatro años la fauna de bosques productivos de la región de Chignahuapan, Puebla. Se registraron 112 especies: 13 de mamíferos medianos y grandes, 83 de aves, cuatro de anfibios y 12 de reptiles. A partir de la abundancia de estas especies observadas, se determinó la riqueza esperada con el estimador no paramétrico Chao1. El método de manejo con mayor riqueza esperada fue el Método de Desarrollo Silvícola (MDS) con 113, seguido por Cortas Sucesivas (CS) con 97, y la menor se observó en el Método Mexicano de Ordenación de Bosques Irregulares (MMOBI) con 71. La completitud de los muestreos varió del 92 % en anfibios al 99 % en mamíferos. Dieciséis especies se encuentran en alguna categoría de riesgo en la norma oficial mexicana NOM-059-SEMARNAT-2010, y 25 son endémicas de México. Se presentan listas taxonómicas con información sobre la situación de conservación, endemismo y abundancia relativa. Los métodos intensivos de manejo forestal, como MDS, tienen una comunidad de mamíferos y aves más rica que el MMOBI y CS, pero similar a las de áreas bajo protección. La riqueza y abundancia de anfibios y reptiles, la mayoría en alguna categoría de riesgo, son bajas, especialmente en el MMOBI y CS, y casi ausentes en zonas de protección.

Abstract Oestrus ovis larvae are obligate parasites of vertebrates and cause cavitary myiasis (oestrosis) in sheep and goats. It is also reported as a zoonosis causing ophthalmomyiasis and nasopharyngeal myiasis. Despite being relatively common in Brazil, epidemiological studies on O. ovis are scarce. Considering that the infestation is influenced by the climate and biomes of the studied region, we aimed to investigate the seasonal prevalence of O. ovis among slaughtered sheep in the northern region of the state of Mato Grosso, Brazil. The heads of sheep (n=697) slaughtered at a slaughterhouse in the municipality of Terra Nova do Norte (November 2011 to November 2013) were collected to count, catalog, and identify the larvae found in the upper respiratory tract. Overall, 45.77% (319/697) of the animals were infested with 2,412 recovered larvae, 96.89% (2,337/2,412) of which were identified at the species level as O. ovis. Seasonal variations in prevalence ranged from 41% (spring) to 56% (summer); however, no correlation was observed between prevalence and season, mean humidity, or temperature. In conclusion, parasitism by O. ovis in sheep in the studied area, occurs year-round, considering the occurrence of larvae (L1, L2, and L3) throughout the year, probably because of the area's environmental conditions. oestrosis (oestrosis goats Brazil O scarce Grosso n=697 n697 n 697 (n=697 201 2013 count catalog tract Overall 4577 45 77 45.77 319/697 319697 319 (319/697 2412 2 412 2,41 9689 96 89 96.89 2,337/2,412 23372412 337 (2,337/2,412 41 spring (spring 56 summer (summer) however season humidity temperature conclusion area yearround, yearround year round, round year-round L1, L1 L (L1 L2 L3 areas s conditions n=69 n69 69 (n=69 20 457 4 7 45.7 319/69 31969 31 (319/69 241 2,4 968 9 8 96.8 2,337/2,41 2337241 33 (2,337/2,41 5 (summer (L n=6 n6 6 (n=6 45. 319/6 3196 3 (319/6 24 2, 96. 2,337/2,4 233724 (2,337/2,4 n= (n= 319/ (319/ 2,337/2, 23372 (2,337/2, (n (319 2,337/2 2337 (2,337/2 (31 2,337/ 233 (2,337/ (3 2,337 23 (2,337 ( 2,33 (2,33 2,3 (2,3 (2, (2

Resumo As larvas de Oestrus ovis são parasitas obrigatórios de vertebrados e causam miíase cavitária (estrose) em ovinos e caprinos. Também é relatada como uma zoonose que causa oftalmomíase e miíase nasofaríngea. Apesar de relativamente comum no Brasil, os estudos epidemiológicos sobre O. ovis são escassos. Considerando que a infestação é influenciada pelo clima e biomas da região estudada, objetivou-se investigar a prevalência sazonal de O. ovis entre ovinos abatidos na região norte do estado de Mato Grosso, Brasil. Foram coletadas cabeças de ovinos (n=697) abatidos em frigorífico do município de Terra Nova do Norte (novembro de 2011 a novembro de 2013) para contagem, catalogação e identificação das larvas encontradas no trato respiratório superior. No geral, 45,77% (319/697) dos animais estavam infestados com 2.412 larvas recuperadas, das quais 96,89% (2.337/2.412) foram identificadas em nível de espécie como O. ovis. As variações sazonais na prevalência variaram de 41% (primavera) a 56% (verão); no entanto, não foi observada correlação entre prevalência e estação do ano, umidade média ou temperatura. Conclui-se que o parasitismo por O. ovis em ovinos na área estudada ocorre durante todo o ano, considerando a ocorrência de larvas (L1, L2 e L3) ao longo do ano, provavelmente devido às condições ambientais da área. estrose (estrose caprinos nasofaríngea Brasil O escassos objetivouse objetivou se Grosso n=697 n697 n 697 (n=697 201 2013 contagem superior geral 4577 45 77 45,77 319/697 319697 319 (319/697 2412 2 412 2.41 recuperadas 9689 96 89 96,89 2.337/2.412 23372412 337 (2.337/2.412 41 primavera (primavera 56 verão (verão) entanto ano temperatura Concluise Conclui L1, L1 L (L1 L3 n=69 n69 69 (n=69 20 457 4 7 45,7 319/69 31969 31 (319/69 241 2.4 968 9 8 96,8 2.337/2.41 2337241 33 (2.337/2.41 5 (verão (L n=6 n6 6 (n=6 45, 319/6 3196 3 (319/6 24 2. 96, 2.337/2.4 233724 (2.337/2.4 n= (n= 319/ (319/ 2.337/2. 23372 (2.337/2. (n (319 2.337/2 2337 (2.337/2 (31 2.337/ 233 (2.337/ (3 2.337 23 (2.337 ( 2.33 (2.33 2.3 (2.3 (2. (2

Abstract High infestations of epibiont ciliates on vertebrates or invertebrates are normally related to aquaculture tanks or similar environments, and the importance of this relationship in natural habitats is often disregarded. Here, we describe the first record of high infestation of ciliates on Aegla serrana in South America and conduct a brief morphological and phylogenetic characterization of these ciliates. Our findings confirm that cases of high infestation of ciliates on metazoans can indeed occur in natural environments. environments disregarded Here

Abstract The progressive fragmentation and loss of habitats represent the main threats for endangered species, causing genetic consequences that may have potential implications for a population’s long-term persistence. Large mammals are the most affected species among vertebrates. The giant armadillo Priodontes maximus is a large South American mammal threatened species, showing nocturnal, solitary and fossorial behavior, occurring at low population densities, and its population dynamics are still poorly known. In this study, we carried out the first assessment of genetic variability and population genetic structure of the species, using a panel of 15 polymorphic microsatellites developed by high-throughput genome sequencing. The spatial Bayesian clustering, Fst and Dest results indicated the presence of two genetic clusters (K = 2) in the study area. These results suggest a reduction in gene flow between individuals inhabiting the Brazilian savanna (Cerrado) and the Pantanal wetlands, with the increased human-driven habitat modifications possibly contributing for this scenario. A bottleneck signal was detected in both populations, and a subpopulation structuring in the Cerrado may also be reflecting consequences of the extensive habitat modifications. Findings from this study provide important and useful information for the future maintenance of genetic diversity and long-term conservation of this flagship species. populations s longterm long term persistence vertebrates nocturnal behavior densities known 1 highthroughput high throughput sequencing clustering K 2 area (Cerrado wetlands humandriven human driven scenario

Abstract Many species of Bromeliaceae store water among their leaves creating microhabitats for several biological groups. Using bromeliads in enrichment of reforestation offers an opportunity to understand the occupation of tank habitat, and the impacts of these plants in increasing biodiversity. We translocated 20 rescued individuals of tank-bromeliad Hohenbergia ramageana to enrich a reforested area in Atlantic Forest. Then, we recorded the visiting dynamics of animals in the tank habitat of H. ramageana. Ten orders of vertebrates and invertebrates visited nine terrestrial and eight epiphyte tank-habitats just one month after the translocation. Richness and composition were similar between epiphyte and terrestrial translocations. We found that precipitation explained a significant proportion of variation of the number of visited plants and richness of animal orders. Our results confirm the importance of using tank-bromeliads in reforested ecosystems and bring novelty that reintroduced bromeliads in alternative habitats also play the same role for animal groups as in their original habitat. This knowledge will orientate future plans of conservation of bromeliads and of ecosystem restoration. biodiversity 2 tankbromeliad bromeliad Forest Then H tankhabitats translocation translocations tankbromeliads restoration

Resumo Muitas espécies de Bromeliaceae armazenam água entre suas folhas criando microhabitats para diversos grupos biológicos. O uso de bromélias no enriquecimento de reflorestamentos oferece uma oportunidade para entender a ocupação do habitat tanque e os impactos dessas plantas no aumento da biodiversidade. Foram translocados 20 indivíduos resgatados da bromélia-tanque Hohenbergia ramageana para enriquecimento de uma área reflorestada de Mata Atlântica. Em seguida, foi gravada a dinâmica de visitação de animais no habitat tanque de H. ramageana. Dez ordens de vertebrados e invertebrados visitaram nove habitats tanques terrícolas e oito epífitos apenas um mês após a translocação. A riqueza e a composição foram semelhantes entre as translocações epífitas e terrícolas. Descobrimos que a precipitação explicou uma proporção significativa de variação do número de plantas visitadas e da riqueza de ordens de animais. Nossos resultados confirmam a importância do uso de bromélias-tanque em ecossistemas reflorestados e trazem novas evidências de que as bromélias reintroduzidas em habitats alternativos também desempenham o mesmo papel para grupos de animais que em seu habitat original. Esse conhecimento orientará planos futuros de conservação de bromélias e de restauração de ecossistemas. biológicos biodiversidade 2 broméliatanque bromélia Atlântica seguida H translocação broméliastanque original

Abstract Neotropical fishes emerge as an extremely diverse group of vertebrates where genomic strategies to evaluate structural and functional features are still beginning. Here, we present a second draft genome of Apareiodon sp. (2n=54, ZZ/ZW), adding PacBio technology whole genome sequencing, and assembling by combining two technologies (long and short reads). Using a detailed strategy for genome assembly with fish genomes of Pygocentrus nattereri, Carassius auratus, and Astyanax mexicanus as references, the final assembly of the Apareiodon sp. genome generated 93 scaffolds, an N50 of 37,200,078 bases, and a size estimate considering 28 scaffolds (26 autosomes+ZW) of ~945 Mb. In Apareiodon sp., this second genome draft confirmed that ~36% of the genome is composed of repetitive DNA. Furthermore, the new draft genome has improved genomic quality assessments, allowing the annotation of 36,290 genes and 15,683 proteins, which presented similarities to reference genomes. The second draft genome of Apareiodon sp. will be useful for research on integrative cytogenetic and genomic data. It will open perspectives for analyzing sex-determining genes in Neotropical fish with a ZZ/ZW sex chromosome system. beginning Here sp 2n=54, 2n54 n 2n 54 (2n=54 ZZ/ZW, ZZZW , ZZ ZW ZZ/ZW) sequencing long reads. reads . reads) nattereri auratus references 9 N N5 37200078 37 200 078 37,200,07 bases 2 26 (2 autosomes+ZW autosomesZW autosomes 945 ~94 Mb 36 ~36 DNA Furthermore assessments 36290 290 36,29 15683 15 683 15,68 proteins data sexdetermining determining system 2n=54 2n5 5 (2n=5 3720007 3 20 07 37,200,0 ( 94 ~9 ~3 3629 29 36,2 1568 1 68 15,6 2n=5 (2n= 372000 0 37,200, ~ 362 36, 156 6 15, 2n= (2n 37200 37,200 3720 37,20 372 37,2 37,

Abstract: Hunting is part of the biocultural heritage of the Maya people of the Yucatán Peninsula because it has been part of their strategy of multiple use of resources and their cosmovision since pre-Hispanic times. The study of knowledge and practices on the uses of natural resources can help to improve the resilience of biological and human communities to disturbances. The aim of this study was to characterize the biocultural knowledge associated with traditional hunting in the Mayan community of Xul, Yucatán. From September to December 2020, 42 mixed questionnaires and seven semi-structured interviews were applied to local hunters. It was found that 23 species of vertebrates (35 mammals, 6 birds, and 1 reptile) are hunted in Xul and are used mainly for food. The species with higher cultural importance (IIC) were the red brocket deer (Mazama temama), collared peccary (Pecari tajacu), and ocellated turkey (Meleagris ocellata). The dry season is their favorite season for hunting (71.4 %) and hunters prefer the milpa (92.9 %) and forest (83.3 %) as hunting sites. The most practiced techniques are the “batida” (flushing by hunting teams, p’uuj; 92.9 %), “caminar por el monte” (walking through the woods, xíimbal ts’on; 73.8 %), and “lampareo” (hunting with flashlights, ts’on; 69 %). In Xul there are beliefs such as virtues, the owners of animals and the winds (Ik’). These results show the continuity of biocultural knowledge related to hunting and its importance in the management and conservation of wild fauna in rural communities.

Resumen: La cacería es una actividad que forma parte del patrimonio biocultural de los mayas de la Península de Yucatán, ya que desde la época prehispánica ha sido parte de su estrategia de uso múltiple de recursos y su cosmovisión. El estudio de los conocimientos y prácticas sobre el empleo de los recursos naturales puede contribuir a aumentar la resiliencia de las comunidades biológicas y humanas ante las perturbaciones. El objetivo de este estudio fue caracterizar el conocimiento biocultural asociado a la cacería tradicional en la comunidad maya de Xul, Yucatán. Se aplicaron 42 cuestionarios mixtos y siete entrevistas semiestructuradas a cazadores locales entre septiembre y diciembre de 2020. Se reportaron 23 especies de vertebrados que se cazan en Xul (16 mamíferos, 6 aves y 1 reptil) y sus usos. Las especies de mayor importancia cultural (IIC) son el venado cabrito (Mazama temama), el jabalí (Pecari tajacu) y el pavo de monte (Meleagris ocellata). La temporada de sequía es la preferida para cazar (71.4 %) y los sitios son la milpa (92.9 %) y el monte alto (83.3 %). Las técnicas que más se practican son la batida (p’uuj; 92.9 %), caminar por el monte (xíimbal ts’on; 73.8 %) y el lampareo (ts’on; 69 %). En Xul existen creencias en elementos y entidades como las virtudes, los Dueños de los animales y los vientos (Ik’). Estos resultados manifiestan la continuidad e importancia de considerar el conocimiento biocultural sobre la cacería con relación al manejo y conservación de la fauna silvestre en comunidades rurales.

Abstract Major histocompatibility complex (MHC) allelic polymorphism is critically important for mediating antigen presentation in vertebrates. Presently, there are insufficient studies of MHC genetic diversity in domestic Anseriform birds. In this study, we analyzed the expression profile of MHC I genes and screened for MHC I exon 2 polymorphism in one domestic goose population from China using Illumina MiSeq sequencing. The results showed that four MHC I alleles (Ancy-IE2*09/*11/*13/*21) in one goose were identified based on cDNA cloning and sequencing using four primer combinations, and the varying number of cDNA clones implied that these four classical sequences showed differential expression patterns. Through next-generation sequencing, 27 alleles were obtained from 68 geese with 3-10 putative alleles per individual, indicating at least the existence of 5 MHC I loci in the goose. The marked excess of the non-synonymous over the synonymous substitution in the peptide-binding region (PBR) along 27 alleles and five positively selected sites (PSSs) detected around the PBR indicated that balancing selection might be the major force in shaping high MHC variation in the goose. Additionally, IA alleles displaying lower polymorphism were subject to less positive selection pressure than non-IA alleles with a higher level of polymorphism. (MHC vertebrates Presently birds study AncyIE2*09/*11/*13/*21 AncyIE209111321 AncyIE Ancy IE2*09/*11/*13/*21 IE2 09 11 13 21 IE (Ancy-IE2*09/*11/*13/*21 combinations patterns nextgeneration next generation 6 310 3 10 3-1 individual nonsynonymous non peptidebinding peptide binding (PBR PSSs (PSSs Additionally nonIA AncyIE2 AncyIE2*09/*11/*13/*2 AncyIE20911132 IE209111321 IE2*09/*11/*13/*2 0 1 (Ancy-IE2*09/*11/*13/*2 31 3- AncyIE2*09/*11/*13/* AncyIE2091113 IE20911132 IE2*09/*11/*13/* (Ancy-IE2*09/*11/*13/* AncyIE2*09/*11/*13/ AncyIE209111 IE2091113 IE2*09/*11/*13/ (Ancy-IE2*09/*11/*13/ AncyIE2*09/*11/*13 AncyIE20911 IE209111 IE2*09/*11/*13 (Ancy-IE2*09/*11/*13 AncyIE2*09/*11/*1 AncyIE2091 IE20911 IE2*09/*11/*1 (Ancy-IE2*09/*11/*1 AncyIE2*09/*11/* AncyIE209 IE2091 IE2*09/*11/* (Ancy-IE2*09/*11/* AncyIE2*09/*11/ AncyIE20 IE209 IE2*09/*11/ (Ancy-IE2*09/*11/ AncyIE2*09/*11 IE20 IE2*09/*11 (Ancy-IE2*09/*11 AncyIE2*09/*1 IE2*09/*1 (Ancy-IE2*09/*1 AncyIE2*09/* IE2*09/* (Ancy-IE2*09/* AncyIE2*09/ IE2*09/ (Ancy-IE2*09/ AncyIE2*09 IE2*09 (Ancy-IE2*09 AncyIE2*0 IE2*0 (Ancy-IE2*0 AncyIE2* IE2* (Ancy-IE2* (Ancy-IE2 (Ancy-IE

Abstract Chromosomal polymorphisms have been reported in numerous vertebrates, including fishes. Such variation is usually related to karyoevolutionary trends and speciation but might also be a result of environmental stress. Herein, we provide the first karyotype characterization in populations of a widespread small characin (Hemigrammus marginatus) from an impacted river basin in Northeastern Brazil to infer their chromosome organization and putative genotoxic effects. Therefore, we reported intra and interindividual structural polymorphism and multiple active nucleolar organizer regions in samples from Contas River, a highly polluted environment when compared to less impacted areas. Furthermore, this report represents the first description of chromosomal mosaicism in the family Characidae so far. Besides revealing peculiar karyotypic patterns in relation to populations from other basins, our results suggest that the chromosomal instability in specimens H. marginatus might be associated with the presence of xenobiotics in polluted environments. vertebrates fishes stress Herein Hemigrammus effects Therefore River areas Furthermore far basins H environments

Abstract The technological development of tools that enable the spawning of different native species is paramount to enable ex situ conservation initiatives, as well as providing means for commercial hatchery of threatened fish which, in turn, relieve fisheries pressure over wild stocks. Neotropical migratory freshwater fish depend on hormonal induction for spawning in hatcheries, through expensive methods of limited efficiency. Salminus brasiliensis is one of the largest Neotropical freshwater fish, a piscivorous top-predator, prized in angling, highly valued in the market, and appreciated in gastronomy. Teleost fish have either, two or three GnRH paralogous genes: GnRH1, GnRH2 and the GnRH3. The expression products of these paralogous isoforms consist of a larger prepro-GnRH polypeptide, which undergoes post-translational proteolytic processing to yield the active decapeptide hormone. There is increasing interest in characterizing and understanding these neuropeptides, because of its practical application in hatchery spawning. We present the characterization of GnRH1’s coding sequence for the prepro-GnRH1 polypeptide of S. brasiliensis. An annotation from a genomic assembly was used for searching for GnRH paralogues, based on data from anonymous predicted transcripts. The sequence retrieved for GnRH1 was then used as a query for searching the uncharacterized GnRH paralogues from full genomes of Characiformes deposited at NCBI. The S. brasiliensis GnRH1 gene sequence retrieved was targeted for PCR and submitted to Sanger sequencing, allowing for its confirmation. It spans 423 bp (exon 1: 128 bp; intron: 161 bp; and exon 2: 1134 bp), with open reading frames coding for 264 and 88 amino acids, respectively. The different variants retrieved for the prepro-GnRH (1, 2 and 3) from Characiformes genomes and deposited sequences from NCBI grouped in three distinct clades in a neighbor joining tree, each forming a monophyletic branch and with the S. brasiliensis sequences nested within the expected groups. Here we observed a variation at a proteolytic site (GKR→GRR), reported as highly conserved in vertebrates up to now, that can potentially alter the cleavage site and modify the peptide topology. This work has characterized, for the first time, the sequence of the GnRH1 coding for its prepro-GnRH peptide, for a member of the Charaficormes order. This will help to promote research and development of tools for broodstock spawning and environmental management of S. brasiliensis and related migratory fish. initiatives turn stocks hatcheries efficiency toppredator, toppredator top predator, predator top-predator angling market gastronomy either genes GnRH3 preproGnRH prepro posttranslational post translational hormone neuropeptides GnRH1s GnRHs s preproGnRH1 S transcripts sequencing confirmation 42 1 12 intron 16 113 bp, , bp) 26 8 acids respectively 1, (1 3 tree groups GKR→GRR, GKRGRR GKR→GRR GKR GRR (GKR→GRR) now topology characterized time order 4 11 ( (GKR→GRR

Resumo O desenvolvimento tecnológico de ferramentas que possibilitem a reprodução de diferentes espécies nativas é fundamental para viabilizar iniciativas de conservação ex situ, bem como fornecer meios para produção comercial de peixes ameaçados que, por sua vez, aliviam a pressão pesqueira sobre os estoques selvagens. Peixes migratórios de água doce Neotropicais dependem de indução hormonal para desova em pisciculturas, com métodos caros e de eficiência limitada. Salminus brasiliensis é um dos maiores peixes de água doce Neotropicais, predador de topo piscívoro, admirado na pesca esportiva, muito valorizado no mercado e apreciado na gastronomia. O hormônio liberador de gonadotrofina (GnRH) é uma molécula-chave na reprodução em todos os vertebrados. Os peixes teleósteos possuem dois ou três genes parálogos de GnRH: GnRH1, GnRH2 e GnRH3. Os produtos de expressão gênica dessas isoformas parálogas consistem em um polipeptídeo maior, pré-pró-GnRH, que sofre processamento proteolítico pós-traducional para produzir o hormônio decapeptídeo ativo. Há um interesse crescente na caracterização e compreensão desses neuropeptídeos, devido à sua aplicação prática na reprodução em pisciculturas. Aqui apresentamos a caracterização da sequência codificadora de GnRH1 para o polipeptídeo pré-pŕo-GnRH1 de S. brasiliensis. Uma anotação de montagem genômica foi usada para procurar parálogos de GnRH, com base em dados de transcritos anônimos preditos em um arquivo de anotação gênica. A sequência recuperada para GnRH1 foi então usada como argumento de busca para procura de parálogos não caracterizados de GnRH, a partir de genomas completos de Characiformes depositados no NCBI. A sequência recuperada de S. brasiliensis do gene GnRH1 foi alvo de PCR e submetida ao sequenciamento Sanger, permitindo a sua confirmação. Esta abrange 423 pb (éxon 1: 128 pb; íntron: 161 pb; éxon 2: 134 pb), com janelas abertas de leitura que codificam para 264 e 88 aminoácidos, respectivamente. As diferentes variantes do pré-pró-GnRH (1, 2 e 3) recuperadas dos genomas completos e sequências depositadas de Characiformes no NCBI agruparam-se em três clados distintos, numa árvore de neighbour joining, cada um formando um ramo monofilético e com as sequências de S. brasiliensis aninhadas dentro dos grupos esperados. Aqui observamos uma variação em um sítio proteolítico (GKR→GRR), até então relatado como altamente conservado em vertebrados, que pode potencialmente alterar o sítio de clivagem e modificar a topologia do peptídeo. Este trabalho caracteriza, pela primeira vez, a sequência do gene GnRH1 que codifica para o peptídeo pré-pró-GnRH1, para um membro da ordem Characiformes. Isto ajudará a promover a pesquisa e o desenvolvimento de ferramentas para a reprodução em piscicultura e a gestão ambiental de S. brasiliensis e outros peixes migratórios relacionados. situ vez selvagens pisciculturas limitada piscívoro esportiva gastronomia GnRH (GnRH moléculachave molécula chave vertebrados GnRH3 maior prépróGnRH, prépróGnRH pré pró póstraducional pós traducional ativo neuropeptídeos prépŕoGnRH1 prépŕoGnRH pŕo pré-pŕo-GnRH S Sanger confirmação 42 1 12 íntron 16 13 pb, , pb) 26 8 aminoácidos respectivamente 1, (1 3 agruparamse agruparam se distintos joining esperados GKR→GRR, GKRGRR GKR→GRR GKR GRR (GKR→GRR) caracteriza prépróGnRH1, prépróGnRH1 pré-pró-GnRH1 relacionados 4 ( (GKR→GRR

Resumen: Ante el cambio climático, las especies pueden perder representación de su nicho en el biotopo, viéndose afectado el riesgo de extinción. Esta breve revisión de la literatura pretende establecer las bases del estado del conocimiento sobre riesgo de extinción en función al nicho climático. Esto permitirá formular futuros abordajes tales como predicciones de riesgo en base al nicho climático. Según la literatura, la amplitud del nicho y la posición relativa de sus componentes determinan el riesgo de extinción. Esta información puede complementar el conocimiento sobre el riesgo de extinción relacionado a la distribución geográfica. También se puede utilizar para planificar estrategias de conservación específicas para cada situación. Las características de los nichos climáticos pueden interactuar con factores externos (amenazas, competencia) e intrínsecos (área ocupada, dispersión), afectando el riesgo de extinción. Aún persisten vacíos de información sobre los nichos climáticos y limitaciones en sus métodos de estudio. La literatura existente se ha enfocado en vertebrados paleárticos y nichos realizados. Por último, se presenta un caso de estudio que ilustra diferencias entre los nichos climáticos realizados de especies de aves según su riesgo de extinción, mediante la comparación de estadísticas descriptivas. Estas confirman en parte lo que menciona la literatura, pero requieren mejoras para garantizar que proporcionen información veraz. En el futuro, sería interesante explorar estas estadísticas como predictores climáticos para conocer el estado de especies poco conocidas y entender mejor el riesgo de extinción, agregando la dimensión climática a las evaluaciones de riesgo.

Abstract: Species could lose part of their niche representation in the biotope due to climate change, potentially affecting extinction risk. This brief review of the literature aims to establish the basic aspects of our current knowledge concerning extinction risk related to climatic niches. This will enable the formulation of future approaches such as predictions of extinction risk based on the climatic niche. The literature indicates that niche width and relative position of niche components determine extinction risk. This information could complement our knowledge of extinction risk based on geographic distributions. It could also be used to plan specific conservation strategies for each situation. Climatic niche characteristics may interact with external factors (threats and competition) and intrinsic factors (occupied area and dispersal), affecting extinction risk. Information gaps about climatic niches persist as well as limitations of their study methods. Most studies have focused on paleartic vertebrates and realized niches. Finally, a case study is presented in which differences among bird realized climatic niches according to their extinction risk are illustrated by comparing descriptive statistics. These partially confirm what is mentioned in the literature but should be improved to guarantee that they provide accurate information. In the future, it would be interesting to explore these descriptive statistics as climatic predictors of conservation status for data deficient species and to gain a better understanding of extinction risk by adding the climatic dimension to risk evaluations.