High-density polyethylene (HDPE) geomembranes are commonly used as an environmental protection liner due to their good chemical and mechanical resistances and low cost. Ultraviolet (UV) radiation is an essential issue in durability studies for pond applications. This study evaluated a 1.5-mm thick HDPE geomembrane exposed to ultraviolet fluorescent radiation for 8760 h in a laboratory and thermoanalytical and physical analyses were conducted towards the understanding of its performance after exposure. According to the results, although the geomembrane maintained the ductile behavior, it showed a 52.48% final decrease in stress crack resistance (SCR) compared to virgin SCR. Moreover, a considerable antioxidant depletion occurred after 8760 h exposure shown by the Std. OIT (standard oxidative-induction time) results, demonstrating a Std. OIT value decrease of 89.19% compared to the virgin Std. OIT. Such a behavior contributed to the susceptibility of thermal effects in the DSC (differential scanning calorimetry) curves and the losses observed in the SCR values, attesting the geomembrane’s oxidative degradation mechanism occurred and changed the polymer’s structure. Highdensity High density (HDPE cost UV (UV applications 1.5mm 15mm mm 1.5 1 5 876 results 5248 52 48 52.48 (SCR Moreover Std standard oxidativeinduction induction time 8919 89 19 89.19 differential calorimetry values s polymers polymer structure 5mm 15 1. 87 524 4 52.4 891 8 89.1 52. 89.

Selenium-containing compounds exhibit diverse biological activities, such as antioxidative, anti-inflammatory, and cancer preventive effects. Using Caenorhabditis elegans as a model organism, we assessed the toxicity, neuroprotective, antioxidant properties, and impact on longevity of 11 selenoacetylenes. Their toxicity and bioactivities varied based on molecular structure. Selenoacetylenes with butyl substituents were toxic to Galleria mellonella larvae. The presence of but-3-in-2-ol radical increased antiprotozoal activity against Tetrahymena pyriformis. Compared to the positive control (Nimitz® EC), selenoacetylenes were less toxic to nematode worms and eggs. Selenoacetylenes significantly reduced amyloid beta (Aβ) paralysis in C. elegans CL4176 worms, increased longevity by 18 to 22%, along with improving survival after oxidative or thermal stress. Galantamine, showed inferior results. These findings enhance our understanding of selenoacetylenes on neuroprotection, antioxidant activity, and longevity in C. elegans. Future mammalian studies will further elucidate mechanisms and explore the potential therapeutic use of selenoacetylenes in treating Alzheimer’s disease and longevity. Seleniumcontaining Selenium containing activities antioxidative antiinflammatory, antiinflammatory anti inflammatory, inflammatory anti-inflammatory effects organism neuroprotective properties 1 structure larvae but3in2ol butinol but 3 2 ol pyriformis Nimitz® Nimitz (Nimitz EC, EC , EC) eggs Aβ (Aβ C CL CL417 22 22% stress Galantamine results neuroprotection Alzheimers Alzheimer s CL41 CL4

Resumen (Objetivo) Tryapanosoma cruzi, un agente causal de la enfermedad de Chagas, es un parásito que presenta una alternancia en su ciclo de vida entre un hospedador invertebrado (triatomino) y uno vertebrado (mamífero). Varios estudios han demostrado que en T. cruzi, HSP83 (homólogo de HSP90) es esencial para la división celular y el control de la respuesta al estrés térmico. Esta investigación se concentró en el estudio de la clonación, la caracterización bioinformática y la expresión del gen de la proteína de choque térmico HSP83 de T. cruzi para estudios posteriores sobre señalización. (Metodología) El ARN fue extraído de epimagostigotes T. cruci (clon EPm6/MOHM/VE/2007/ 6c), utilizando un kit comercial. El ADNc que codifica HSP83 se obtuvo utilizando RT-PCR, a partir del ARNm extraído, para lo cual se diseñaron los cebadores con base en la secuencia HSP83 de la cepa T. cruzi CL Brener. La clonación se realizó usando pGEM®T-Easy y se subclonó en el vector de expresión pQE30. Se efectuó la caracterización bioinformática y de secuencias. El gen se expresó y la proteína recombinante se purificó por cromatografía de afinidad, igual que se identificó por inmunotransferencia. (Resultados) El análisis de secuencia mostró similitud con el gen HSP83 de Trypanosoma cruzi y se observaron dominios HSP, así como epítopos B en la secuencia. Después de 3 horas de inducción con IPTG, se obtuvo una proteína recombinante con un peso aproximado de 83 kDa. La reacción de inmunotransferencia con suero hiperinmune anti-epimastigote de T. cruzi permitió la detección de una sola banda con un peso molecular de aproximadamente 83 kDa. (Conclusiones) Todos los resultados indican que se logró la clonación y caracterización de HSP83 de Trypanosoma cruzi.

Resumo (Objetivo) O Tryapanosoma cruzi, agente causador da doença de Chagas, é um parasita que apresenta uma alternância em seu ciclo de vida entre um hospedeiro invertebrado triatomíneo) e um vertebrado(mamífero). Vários estudos demonstraram que, no T. cruzi, a HSP83 (homóloga da HSP90) é essencial para a divisão celular e o controle da resposta ao estresse térmico. Esta pesquisa concentrou-se no estudo da clonagem, caracterização bioinformática e expressão do gene da proteína de choque térmico HSP83 do T. cruzi para estudos adicionais de sinalização. (Metodologia) O RNA foi extraído de epimastigotas de T. cruzi (clone EPm6/MOHM/VE/2007/ 6c) usando um kit comercial. O cDNA que codifica a HSP83 foi obtido por meio de RT-PCR a partir do mRNA extraído, para o qual os primers foram projetados com base na sequência da HSP83 da cepa T. cruzi CL Brener. A clonagem foi realizada com o pGEM®T-Easy e subclonada no vetor de expressão pQE30. Foi realizada a caracterização bioinformática e de sequência. O gene foi expresso e a proteína recombinante foi purificada por cromatografia de afinidade e identificada por imunotransferência. (Resultados) A análise da sequência mostrou similaridade com o gene HSP83 do Trypanosoma cruzi e os domínios HSP, bem como os epítopos B, foram observados na sequência. Após 3 horas de indução com IPTG, foi obtida uma proteína recombinante com peso aproximado de 83 kDa. A imunotransferência com soro hiperimune antiepimastigota de T. cruzi permitiu a detecção de uma única banda com peso molecular de aproximadamente 83 kDa. (Conclusões) Todos os resultados indicam que a clonagem e a caracterização da HSP83 do Trypanosoma cruzi foram alcançadas.

Abstract (Objective) Trypanosoma cruzi, a causal agent of Chagas’ disease, is a parasite whose life cycle alternates between an invertebrate (triatomine) and a vertebrate (mammal) host. Various studies have shown that in T. cruzi, HSP83 (a homolog of HSP90) is essential for cell division and control of the response to thermal stress. This investigation focused on studying the cloning, bioinformatic characterization, and expression of the heat shock protein HSP83 T. cruzi gene for further cell signaling studies. (Methodology) RNA was extracted from T. cruzi epimastigotes (EPm6 clone, MHOM/VE/2007/ 6c) using a commercial kit. The cDNA encoding HSP83 was determined using RT-PCR on extracted mRNA, for which the primers were designed based on the HSP83 sequence of T. cruzi strain CL Brener. Cloning was performed using pGEM®T-Easy and subcloned into the expression vector pQE30. Sequence and bioinformatic characterization were performed. The gene was expressed, and the recombinant protein was purified using affinity chromatography and identified through immunoblotting. (Results) Sequence analysis showed similarity to the gene encoding HSP83 from Trypanosoma cruzi, and HSP domains and B epitopes in the sequence were also observed. After 3 hours of induction with IPTG, a recombinant protein with an approximate weight of 83 kDa was obtained. The immunoblotting reaction with hyperimmune anti-T. cruzi epimastigote serum helped detect a single band with a molecular weight of nearly 83 kDa. (Conclusions) All results indicate that the cloning and characterization of HSP83 from Trypanosoma cruzi was achieved.

ABSTRACT The production process of non-centrifuged cane sugar (NCS) involves a significant release of steam and heat due to the evaporation of cane juices in saucepans over a solid fuel oven. This results in a warm and moisture-saturated environment, which can be suffocating for workers. This study aimed to evaluate the bioclimatic behavior of an NCS processing facility, based on the wet bulb globe temperature (WBGT) index. In addition, solutions are suggested to mitigate possible adverse effects of heat stress. This evaluation utilized computational simulations to determine the thermal comfort perceived by the workers. The accuracy of simulations was verified against temperature and relative air humidity data collected from the facility. Bioclimatic simulations encompassed twelve treatments, involving modifications to the enclosure on the walls and lantern window, incorporating three types of roof material. The WBGT index was determined by considering the effects of the radiant heat generated by the oven and the natural ventilation area on the facility’s temperature and relative air humidity. This helps to assess the comfort experienced by the workers. The thermal zone of the oven presented heat stress conditions; therefore, rest periods and mechanical ventilation were suggested when dissipating heat and steam through natural ventilation is not possible. For workers exposed to high temperatures and thermal radiation, the use of an aluminized apron and infrared goggles for eye protection was recommended. noncentrifuged non centrifuged (NCS moisturesaturated moisture saturated environment facility (WBGT addition treatments window material facilitys s conditions therefore radiation recommended

RESUMO O processo de produção de rapadura gera alta concentração de vapor e calor devido à evaporação do caldo da cana em panelas sobre forno a combustível sólido. Isso gera um ambiente quente e saturado de umidade que pode ser sufocante para os trabalhadores. Este estudo teve como objetivo avaliar as condições ambientais de uma instalação de processamento de rapadura, com base no Índice de Bulbo Úmido e Temperatura de Globo (IBUTG) e sugerir soluções para mitigar possíveis efeitos adversos do estresse térmico. Esta avaliação utilizou simulação computacional para determinar o conforto térmico dos trabalhadores. A simulação foi validada com dados de temperatura e umidade relativa do ar da instalação. As simulações bioclimáticas foram realizadas em doze tratamentos, modificando o fechamento nas paredes e lanternim, com três tipos de materiais de cobertura. Foi determinado o Índice IBUTG, considerando os efeitos do calor radiante gerado pelo forno, e pela área de ventilação natural sobre a temperatura e umidade relativa do ar no interior da instalação, e seu efeito no conforto dos trabalhadores. A zona térmica do forno apresentou condições de estresse térmico, portanto, foram sugeridos períodos de descanso e ventilação mecânica quando não for possível a evacuação do calor e vapor através da ventilação natural. O uso de avental aluminizado e óculos infravermelhos para proteção ocular foi recomendado para os trabalhadores expostos a altas temperaturas e radiação térmica. sólido IBUTG (IBUTG tratamentos lanternim cobertura portanto

RESUMEN Antecedentes: El nivel de carga calórica acumulada del ITH (CCA) durante los 6 meses previos al destete sobre el peso al destete (PD), fue evaluado en animales Charoláis como expresión de stress térmico (ST), así como su posible componente genético. Métodos: Se analizaron 19 466 registros de PD de la Empresa Manuel Fajardo, Jiguaní, Granma, nacidos entre 1988 y 2017 en 16 rebaños. Se utilizó un modelo de Norma de Reacción considerando efecto fijo cuatrimestres-años-rebaños (859 clases), sexo del ternero (2) y número de partos de la madre (11), una covariable fija mediante un polinomio de Legendre (L) de orden 3 para representar la curva de respuesta del PD en la trayectoria de CCA. Como aleatorios: residuo y la desviación del animal que produce el registro que se modeló por un L de orden 1. Con los valores genéticos estandarizados (VG) se hizo un análisis de componentes principales y con los coeficientes de los eigenvector se construyó un índice de los resultados. Resultados: El análisis de regresión arrojó que el PD se deprime en b=-0,807±0,04 kg por unidad de CCA (R2=90,2%). Se diferenciaron tres zonas: fría CCA<=439; neutra CCA>=440' a <=450 y caliente CCA >=451, con PD= 149,3; 140,5 y 134,1 kg respectivamente. La variación en VG es mayor en la zona fría, pero las correlaciones fueron muy bajas (0,33) evidenciando interacción genotipo ambiente (Ige). Conclusiones: Se demostró existencia de una base genética para tolerancia a ST con manifestaciones de Ige, y cambia el orden de mérito en condiciones extremas.

ABSTRACT Background: The level of accumulated caloric load of the ITH (CCA) during the 6 months prior to weaning on the weaning weight (PD) was evaluated in Charolais animals as an expression of thermal stress (ST), as well as its possible genetic component. Methods: 19,466 PD records from the Manuel Fajardo Enterprise, Jiguaní, Granma, born between 1988 and 2017 in 16 herds, were analyzed. A Reaction Norm model was used considering a fixed effect of quarters-years-herds (859 classes), sex of the calf (2) and number of calving of the mother (11), a fixed covariate using a Legendre polynomial (L) of order 3 to represent the PD response curve in the CCA trajectory. As random: residual and the deviation of the animal that produces the record that was modeled by an L of order 1. With the standardized genetic values (VG) a principal components analysis was done and with the eigenvector coefficients an index of the results. Results: The regression analysis showed that PD is depressed by b=-0,807±0,04 kg per unit of CCA (R2=90,2%). 3 zones were differentiated: cold CCA<=439; neutral CCA>=440 to <=450 and hot CCA>=451, with PD= 149,3; 140,5 and 134,1 kg respectively. The variation in VG is greater in the cold zone, but the correlations were very low (0,33), evidencing genotype-environment interaction (Ige). Conclusions: The existence of a genetic basis for tolerance to ST with Ige manifestations was demonstrated, and the order of merit changes in extreme conditions.

An article in the July/August 2022 issue (Havenga et al., S Afr J Sci. 2022;118(7/8), Art. #13118) argued that changing the date of the Comrades Marathon from May to August would result in increased heat stress for participants. Heat stress was estimated using the Universal Thermal Climate Index (UTCI), which is designed to represent a person walking, not running. In this Commentary, I argue that using the UTCI may lead to an underestimation of heat stress for the Comrades Marathon, and that the conclusion that August has worse heat stress than May depends on the assumptions in the estimation of heat stress

We value comments on our research paper in a Commentary in this issue (Simpson, S Afr J Sci. 2024;120(1/2), Art. #16445). Acknowledging the Universal Thermal Comfort Index (UTCI)'s limitations in capturing individual physiological responses remains important; however, we argue for its appropriateness based on recent thermophysiology and heat exchange advancements during its development and broader alignment with standardised indexing efforts. Our original research paper set out with these considerations in mind, and our conclusions remain valid. We further argue for refinement of the UTCI for specific activities instead of using the RET. Finally, future efforts should focus on monitoring data in real-world scenarios to validate and improve thermal indices

Abstract In view of the growing scarcity of water for agriculture, the increase in food demand and future drought scenarios posed by climate change, it is essential to design new technologies that contribute to lower water consumption. In this research, high-resolution images have been used to estimate evapotranspiration in rice fields by applying the METRICTM (Mapping Evapotranspiration at High Resolution using Internalized Calibration) energy balance model. For this purpose, 5900 m2 of crop were monitored under continuous flood irrigation (CF) and 2600 m2 under alternate wetting and drying irrigation (AWD), in addition to some plots with lateral filtration. Ten flights were conducted between tillering and flowering phases, five flights with a Matrice 210 UAV equipped with a Parrot Sequoia multispectral camera, and five flights with a Matrice 300 RTK equipped with a H20T thermal camera. Field data were collected from vegetation indices (NDVI and LAI), and readings from a radiometer, to adjust information from multispectral and thermal images, respectively, and to obtain the components of the surface energy balance. Mean values for crop evapotranspiration (ETc) of 6.34 ± 1.49 and 5.84 ± 0.41 mm d-1 were obtained for IC irrigation and AWD irrigation, respectively, obtaining a water saving of 42% with a yield reduction of 14%, providing a guide for proper irrigation management, however, it is suggested to use the model to optimize yield by obtaining critical thresholds for optimal application of AWD in the face of water resource scarcity.

Resumen Ante la creciente escasez del agua para la agricultura, el incremento de la demanda de alimentos y los futuros escenarios de sequía que nos plantea el cambio climático es indispensable diseñar nuevas tecnologías que contribuyan a un menor consumo de agua. En esta investigación se han empleado imágenes de alta resolución para estimar la evapotranspiración en arrozales aplicando el modelo de balance de energía METRICTM (Mapping Evapotranspiration at High Resolution using Internalized Calibration). Para ello, se monitorizaron 5900 m2 de cultivo bajo riego por inundación continua (IC) y 2600 m2 bajo la técnica de riego de alternancia humedecimiento y secado (AWD, por sus siglas en inglés), además de algunas parcelas con filtración lateral. Se realizaron 10 vuelos entre las etapas de macollamiento y floración, cinco vuelos con un VANT Matrice 210 con una cámara multiespectral Parrot Sequoia, y cinco vuelos con un Matrice 300 RTK equipado con una cámara térmica H20T. Se colectó información de campo de los índices de vegetación (NDVI e IAF), y lecturas de un radiómetro, para ajustar información de las imágenes multiespectrales y térmicas, respectivamente; y obtener los componentes del balance de energía en superficie. Se obtuvo valores medios para evapotranspiración del cultivo (ETc) de 6,34 ±1,49 y 5,84 ± 0,41 mm d-1 para riego IC y riego AWD, respectivamente, obteniéndose un ahorro de agua del 42% con una reducción del rendimiento en 14%, proporcionando una guía para la gestión adecuada del riego, sin embargo, se sugiere utilizar el modelo para optimizar el rendimiento obteniendo umbrales críticos para la aplicación óptima de AWD frente a la escasez del recurso hídrico.

ABSTRACT Climate change continues to influence global ecosystems, raising concerns for livestock. This study assesses the impacts of climate change on broiler chickens in northern Tunisia, focusing on well-being and mortality rates during summer. Historical data from the NRMCM5.1 and MPIESM1.2 models, were utilized, covering 1970 to 1997. Projections for 2041-2070 under the RCP4.5 and RCP8.5 emissions scenarios were examined, providing insight into future challenges. The Temperature-Humidity Index (THI) and Temperature-Humidity-Velocity Index (THVI) served as thermal comfort indicators. The research utilized temperature and relative air humidity data from two models and scenarios (RCP4.5 and RCP8.5) as inputs for the DCP system, thus evaluating comfort parameters (THI and THVI). The analysis involved calculating annual temperature and humidity averages at the system’s output for each grid and region. Historical and projected data were employed to assess mortality levels by identifying heatwave periods, which had an average duration of 2.7 consecutive days with THI exceeding 30.6°C. The analysis showed significant increases in THI and THVI in the RCP8.5 pessimistic scenario, indicating a risk of heat stress. Mortality rates were used as a measure of the vulnerability of the poultry industry to climate change, and the projections showed substantial average increases of 2.2°C for THI and 1.5°C for THVI.. The RCP4.5 and RCP8.5 scenarios predicted an increase in mortality for the period 2041-2070, with averages increasing from 0.8 to 1.3 for RCP8.5 and from 0.6 to 1.1 for RCP4.5, highlighting the need for adaptation strategies to ensure sustainability in poultry farming. ecosystems livestock Tunisia wellbeing well being summer NRMCM51 NRMCM NRMCM5 1 NRMCM5. MPIESM12 MPIESM MPIESM1 2 MPIESM1. 197 1997 20412070 2041 2070 2041-207 RCP45 RCP RCP4 5 RCP4. RCP85 RCP8 RCP8. examined challenges TemperatureHumidity Temperature Humidity TemperatureHumidityVelocity Velocity (THVI indicators (RCP4. system THVI. . THVI) systems s region periods 27 7 2. 306C C 30 6 30.6°C scenario stress 22C 15C 20412070, 2070, 08 0 8 0. 13 3 1. 06 11 farming 19 199 2041207 204 207 2041-20 (RCP4 204120 20 2041-2 (RCP 20412 2041-

Abstract This study aimed to calculate the temperature and humidity indexes (THIs) derived from extremely high air temperatures during the months of December 2021, January, and February 2022 in Rio Grande do Sul. The main goal was to characterize and regionalize potential impacts on dairy production. We used hourly measurements of temperature and relative humidity from 28 meteorological stations of the INMET/SIMAGRO/SEAPI network. These data cover ten ecoclimatic regions of the state. THI and Estimated Milk Loss (EML) were calculated, with the daily number of hours classified under each THI category during the trimester by municipality and region. The effects of region and month on THI and EML were evaluated through analysis of variance at a 5% significance level. Differences between means were compared using the Tukey HSD test (P<0.05). The probability of each daily hour having a THI in thermal discomfort (THI>70) was determined through analysis of variance for binomial variables by region, with the observed effect of time (P<0.05), and means were compared using the non-parametric Bonferroni test at 5%. To group these data, we applied the ScottKnott test. The Baixo Vale do Uruguai stood out in the trimester with the highest THI values, indicating thermal discomfort. Conversely, no heat stress was indicated in the Serra do Nordeste. In all regions, the most frequent daily classification of thermal discomfort ranged from attention to alert, with January being particularly significant. The highest EML estimates for most of the eight production levels were recorded in January. High estimates of productivity loss occurred in cows with higher milk production potential. THIs (THIs 2021 202 Sul 2 INMETSIMAGROSEAPI INMET SIMAGRO SEAPI network state (EML calculated 5 level P<0.05. P005 P P<0.05 . 0 05 (P<0.05) THI>70 THI70 70 (THI>70 P<0.05, , nonparametric non parametric values Conversely Nordeste alert significant 20 P00 P<0.0 (P<0.05 THI>7 THI7 7 (THI>7 P0 P<0. (P<0.0 THI> (THI> P<0 (P<0. (THI P< (P<0 (P< (P

Resumo O objetivo do estudo foi calcular o índice de temperatura e umidade (ITU), devido às temperaturas do ar extremamente elevadas durante os meses de dezembro 2021, janeiro e fevereiro 2022 no Rio Grande do Sul, para fins de caracterização e regionalização dos possíveis impactos na produção leiteira. Empregaram-se dados horários de temperatura e umidade relativa do ar de 28 estações meteorológicas da rede INMET/SIMAGRO/SEAPI, de dez regiões ecoclimáticas do Estado, calculando-se ITU e Perda Estimada de Leite (DPL), contabilizando-se número diário de horas em cada classificação do ITU, durante trimestre por município e região. Avaliou-se efeito da região e mês no ITU e DPL, pela análise de variância a 5% de significância. Detectadas diferenças entre médias, compararam-se pelo teste Tukey HSD (P<0,05). Verificou-se probabilidade de cada hora diária apresentar ITU em desconforto térmico (ITU>70), através da análise de variância para variáveis binomiais, por região; observado efeito de horário (P<0,05), médias foram comparadas pelo teste não paramétrico Bonferroni a 5%. Para agrupamento destas, usou-se o teste Scott-Knott. O Baixo Vale do Uruguai se destacou no trimestre com maiores valores do ITU, desconforto térmico, enquanto na Serra do Nordeste, não indicaram estresse calórico. Em todas as regiões, a classificação de desconforto térmico mais frequente, diariamente, foi de atenção até alerta, destacando-se o mês de janeiro. Maiores estimativas de DPL, para grande parte dos oito níveis de produção, registraram-se em janeiro. Elevadas estimativas de perda de produtividade, ocorreram em vacas com maior potencial de produção de leite. , (ITU) 2021 202 Sul leiteira Empregaramse Empregaram 2 INMETSIMAGROSEAPI INMET SIMAGRO SEAPI INMET/SIMAGRO/SEAPI Estado calculandose calculando DPL (DPL) contabilizandose contabilizando Avaliouse Avaliou 5 significância compararamse compararam P<0,05. P005 P P<0,05 . 0 05 (P<0,05) Verificouse Verificou ITU>70, ITU70 ITU>70 70 (ITU>70) binomiais P<0,05, destas usouse usou ScottKnott. ScottKnott Scott Knott. Knott Scott-Knott Nordeste calórico frequente diariamente alerta destacandose destacando registraramse registraram produtividade leite (ITU 20 (DPL P00 P<0,0 (P<0,05 ITU7 ITU>7 7 (ITU>70 P0 P<0, (P<0,0 ITU> (ITU>7 P<0 (P<0, (ITU> P< (P<0 (P< (P

Abstract Human activities are altering the existing patterns of Land Use Land Cover (LULC) and Land Surface Temperature (LST) on a global scale. However, long-term trends of LULC and LST are largely unknown in many remote mountain areas such as the Karakorum. . The objective of our study therefore was to evaluate the historical changes in land use and land cover (LULC) in an alpine environment located in Islamabad Capital Territory, Pakistan. We used Landsat satellite pictures (namely Landsat 5 TM and Landsat 8 OLI) from the years 1988, 2002, and 2016 and applied the Maximum Likelihood Classification (MLC) approach to categorize land use classes. Land Surface Temperatures (LST) were calculated using the thermal bands (6, 10, and 11) of Landsat series data. The correlation between the Human Modification Index (HMI) and LULC as well as LST was evaluated by utilizing data from Google Earth Engine (GEE). Over the study period, the urbanized area increased by 9.94%, whilst the agricultural and bare soil areas decreased by 3.81% and 3.94%, respectively. The findings revealed a significant change in the LULC with a decrease of 1.99% in vegetation. The highest LST class exhibited a progressive trend, with an increase from 12.27% to 48.48%. Based on the LST analysis, the built-up area shows the highest temperature, followed by the barren, agricultural, and vegetation categories. Similarly, the HMI for different LST categories indicates that higher LST categories have higher levels of human alteration compared to lower LST categories, with a strong correlation (R-value = 0.61) between HMI and LST. The findings can be utilized to promote sustainable urban management and for biodiversity conservation efforts. The work also has the potential of utilizing it to protect delicate ecosystems from human interference and to formulate strategies and regulations for sustainable urban growth, including aspects of land utilization and zoning, reduction of urban heat stress, and urban infrastructure. (LULC (LST scale However longterm long term Karakorum Territory Pakistan namely OLI 1988 2002 201 MLC (MLC classes 6, 6 (6 10 11 (HMI GEE. GEE (GEE) period 994 9 94 9.94% 381 3 81 3.81 394 3.94% respectively 199 1 99 1.99 trend 1227 12 27 12.27 4848 48 48.48% analysis builtup built up temperature barren Similarly Rvalue R value 0.61 061 0 61 efforts growth zoning stress infrastructure 198 200 20 ( (GEE 9.94 38 3.8 39 3.94 19 1.9 122 2 12.2 484 4 48.48 0.6 06 9.9 3. 3.9 1. 12. 48.4 0. 9. 48.

Resumo As atividades humanas estão alterando os padrões existentes de uso e cobertura do solo (LULC) e temperatura da superfície terrestre (TST) em escala global. No entanto, as tendências a longo prazo de LULC e LST são, em grande parte, desconhecidas em muitas áreas montanhosas remotas, como em Karakorum. O objetivo do nosso estudo foi, portanto, avaliar as mudanças históricas no uso e cobertura da terra (LULC) em um ambiente alpino localizado no Território da Capital Islamabad, Paquistão. Utilizamos imagens de satélite Landsat (nomeadamente Landsat 5 TM e Landsat 8 OLI) dos anos de 1988, 2002 e 2016 e aplicamos a abordagem de Classificação de Máxima Verossimilhança (MLC) para categorizar as classes de uso da terra. As temperaturas da superfície terrestre (TST) foram calculadas usando as bandas térmicas (6, 10 e 11) dos dados da série Landsat. A correlação entre o Índice de Modificação Humana (HMI) e o LULC, bem como o LST, foi avaliada utilizando dados do Google Earth Engine (GEE). Durante o período de estudo, a área urbanizada aumentou 9,94%, enquanto as áreas agrícolas e de solo descoberto diminuíram 3,81% e 3,94%, respectivamente. Os resultados revelaram uma mudança significativa no LULC, com uma diminuição de 1,99% na vegetação. A classe LST mais alta apresentou tendência progressiva, com aumento de 12,27% para 48,48%. Com base na análise do LST, a área construída apresentou a temperatura mais elevada, seguida pelas categorias árida, agrícola e vegetação. Da mesma forma, o HMI para diferentes categorias de LST indicou que as categorias mais altas de LST apresentaram níveis mais elevados de alteração humana em comparação com as categorias mais baixas de LST, com uma forte correlação (valor R = 0,61) entre HMI e LST. As conclusões podem ser tomadas como base para promover a gestão urbana sustentável e para esforços de conservação da biodiversidade. O trabalho também tem o potencial de ser utilizado para proteger ecossistemas delicados da interferência humana e para formular estratégias e regulamentos para o crescimento urbano sustentável, incluindo aspectos de utilização e zoneamento da terra, redução do estresse térmico urbano e infraestrutura urbana. (LULC TST (TST global entanto são parte remotas Karakorum portanto Islamabad Paquistão nomeadamente OLI 1988 200 201 MLC (MLC 6, 6 (6 1 11 (HMI GEE. GEE . (GEE) 994 9 94 9,94% 381 3 81 3,81 394 3,94% respectivamente 199 99 1,99 vegetação progressiva 1227 12 27 12,27 4848 48 48,48% elevada árida forma valor 0,61 061 0 61 biodiversidade 198 20 ( (GEE 9,94 38 3,8 39 3,94 19 1,9 122 2 12,2 484 4 48,48 0,6 06 9,9 3, 3,9 1, 12, 48,4 0, 9, 48,

Abstract Omeprazole (OM), a temperature, pH, and moisture-sensitive drug, poses formulation challenges. This study delves into the complex development of OM pellets, focusing on the impact of buffering excipients on stability and release. Addressing the challenges of OM pellet stability requires a comprehensive compatibility and stress study involving key excipients. Binary mixtures of OM with dibasic sodium phosphate dihydrate (DSPD), mannitol, hypromellose (Hyp), and polysorbate underwent scrutiny for possible incompatibilities, subjected to 40°C stress and 75% relative humidity. DSC, TGA, and ATR-FTIR analyses were conducted, with quantitative monitoring of OM by HPLC. Formulations with varied proportions of DSPD and Hyp were also stress-tested. While all excipients exhibited compatibility with OM, thermal analysis suggested a potential incompatibility between OM and mannitol, disproven by HPLC. Stress tests on diverse formulations confirmed their adequacy, maintaining OM content and impurities within acceptable limits. Increased Hyp reduced impurities, and its combination with DSPD further enhanced stability. The study concludes that augmenting DSPD with Hyp offers effective protection for OM pellets, ensuring their stability. , (OM) temperature pH moisturesensitive moisture sensitive drug pellets release DSPD, (DSPD) mannitol Hyp, (Hyp) incompatibilities 40C C 40 75 humidity DSC TGA ATRFTIR ATR FTIR conducted HPLC stresstested. stresstested tested. tested stress-tested adequacy limits (OM (DSPD (Hyp 4 7

This study assessed the morphology and thermal, mechanical, and chemical properties of electrospun membranes made of acrylonitrile-methacrylic acid copolymers (PANCMAA). Copolymers were synthesized using the aqueous suspension method with varying comonomer contents (homopolymer,PAN, lower acid, PANCMAA1, and higher acid, PANCMAA2) and characterized by nuclear magnetic resonance (NMR) spectroscopy and gel permeation chromatography (GPC). Electrospun fibers were produced by adjusting the polymer concentration in the DMF solution, and the optimal conditions yielded nanofibrous membranes. PAN and PANCMAA membrane properties were evaluated by X-ray diffraction (XRD), differential scanning calorimetry (DSC), Dynamic Mechanical Thermal Analysis (DMTA), stress-strain tests, and swelling behavior in a phosphate buffer solution. The comonomer content influenced the crystallinity of the electrospun nanofibers, reducing the 16.8° XRD peak intensity from PAN to PANCMAA2. DSC indicated an increased Tg (from 113°C in PAN to 127°C in PANCMAA2) and a decreased exothermic cyclization enthalpy (311 J.mg-1 in PAN to 271 J.mg-1 in PANCMAA2). PANCMAA1 exhibited higher stiffness than PANCMAA2 and PAN, with a storage modulus of 0.16 GPa, a Young’s modulus of 135.7 MPa, and a lower elongation at break (3.98%). The presence of the acid comonomer significantly enhanced the swelling behavior of the electrospun membranes in phosphate buffer solution. thermal mechanical acrylonitrilemethacrylic acrylonitrile methacrylic PANCMAA. . (PANCMAA) homopolymer,PAN, homopolymerPAN homopolymer (homopolymer,PAN NMR (NMR GPC. GPC (GPC) solution Xray X ray XRD, , (XRD) DSC, (DSC) DMTA, DMTA (DMTA) stressstrain stress strain tests nanofibers 168 16 8 16.8 113C C 113 127C 127 311 (31 J.mg1 Jmg1 Jmg J.mg 1 J mg J.mg- 27 016 0 0.1 GPa Youngs Young s 1357 135 7 135. MPa 3.98%. 398 3.98% 3 98 (3.98%) (PANCMAA homopolymer,PAN (GPC (XRD (DSC (DMTA 16. 11 12 31 (3 mg1 2 01 0. 13 39 3.98 9 (3.98% ( 3.9 (3.98 3. (3.9 (3.

ABSTRACT In recent years the concern of environmentalists has been growing due to the large use of products that have non-renewable fossil sources as raw material. An alternative adopted by the researchers is the production of thermoplastic matrix composites with lignocellulosic waste fibers. The oticica or oiti is a fruit rich in oil and is widely used for soap production. The co-product generated from this process is rich in fiber, oil and impurities and is currently widely used for animal feed and soil fertilizer. The objective of the present work is the production and evaluation of the properties of HDPE composites with Oiticica waste. As matrix was used a green HDPE supplied by Braskem and the dispersed phase was the pie de oiticica supplied by a soap factory. Compositions with 5, 10 and 20% of waste de oiticica were processed by double screw extrusion and the specimens made by the injection process. The composites were characterized by uniaxial tensile test to evaluate mechanical properties (deformation to rupture, modulus, and stress at maximum force). The interaction between pie and matrix was analyzed through the fracture surface using scanning electron microscopy and the analysis. Thermal analysis was assessed by DSC. The results showed decrease in the deformation for 10 and 20% waste compositions. There was a gradual decrease at the stress at the maximum strength of the composites when compared to HDPE. The micrographs showed little interaction between fiber and matrix and the presence of voids. Thermal analysis showed a decrease in the degree of crystallinity, concluding that the presence of the oiticica may have made it difficult to organize the chains, generating a greater number of amorphous regions. nonrenewable non renewable material fibers coproduct co product fertilizer factory 5 1 20 rupture modulus force. force . force) DSC compositions voids crystallinity chains regions 2

RESUMO Nos últimos anos a preocupação de ambientalistas vem crescendo devido ao grande uso de produtos que tem como matéria-prima fontes fósseis não renováveis. Uma alternativa adotada entre os pesquisadores é a produção de compósitos de matriz termoplástica com fibras de resíduos de lignocelulósicas. A oiticica ou oiti é um fruto rico em óleo e é muito utilizado para a produção de sabão. O co-produto gerado desse processo é rico em fibras, óleo e impurezas e atualmente é muito utilizado para ração animal e adubo para solo. O objetivo do presente trabalho é a produção e avaliação das propriedades de compósitos de PEAD com Torta de Oiticica. Como matriz foi utilizado um PEAD verde fornecido pela Braskem e a fase dispersa foi a Torta de oiticica fornecida por uma fábrica de sabão. As composições com 5, 10 e 20% de Torta de oiticica foram processadas por extrusão dupla rosca e os corpos de provas confeccionados pelo processo de injeção. Os compósitos foram caracterizados através do ensaio de tração uniaxial para avaliação de propriedades mecânicas (deformação até a ruptura, módulo e tensão na força máxima). A interação entre torta e matriz foi analisada através da superfície de fratura utilizando microscopia eletrônica de varredura e a análise térmica foi avaliada através de DSC. Os resultados mostraram diminuição na deformação para composições com 10 e 20% de torta. Houve uma queda gradativa na tensão na força máxima nos compósitos quando comparada com a do PEAD. As micrografias mostraram pouca interação entre fibra e matriz e a presença de vazios. As análises térmicas apresentaram uma diminuição no grau de cristalinidade. concluindo-se que a presença da torta pode ter dificultado a organização das cadeias, gerando maior número de regiões amorfas. matériaprima matéria prima renováveis lignocelulósicas sabão coproduto co produto solo Oiticica 5 1 20 injeção ruptura máxima. . máxima) DSC vazios cristalinidade concluindose concluindo se cadeias amorfas 2

ABSTRACT Enthalpy is currently suggested as a suitable index for assessing the internal environment of poultry production, primarily dependents on air temperature and relative air humidity. This study aimed to analyze enthalpy in bird production in Londrina, located in the northern region of Paraná, Brazil, and to model the comfort enthalpy required for enhanced production efficiency. Data from 1961 to 2021 from the Instituto Nacional de Meteorologia (INMET), Brasília, Federal District, Brazil, were utilized to compare the thermal comfort conditions and critical limits set for the birds. The meteorological data from this period were scrutinized to understand their variations and interrelationship through a descriptive study and correlation analysis of different parameters. The research findings, based on the enthalpy indices derive historical averages, suggest that heating is crucial for birds during the first and second weeks of housing, during the winter, given the location’s low temperatures. For birds housed during the third to sixth weeks of age, the enthalpy indices indicated the necessity of cooling systems. humidity Londrina Paraná Brazil efficiency 196 202 INMET, INMET , (INMET) Brasília District parameters findings averages housing winter locations location s temperatures age systems 19 20 (INMET 1 2

RESUMO A entalpia tem sido proposta atualmente como um índice adequado para a avaliação do ambiente interno de galpões de frangos de corte, pois depende basicamente da temperatura e da umidade relativa do ar. Dessa forma, objetivou-se analisar a entalpia como índice de conforto térmico na produção de aves para o município de Londrina, Paraná, e modelar a entalpia aos índices de conforto térmico que proporcionam eficiência na produção. Para o estudo, utilizaram-se dados de 1961 a 2021 do Instituto Nacional de Meteorologia (INMET), de Brasília, Distrito Federal. Os dados meteorológicos desse período foram analisados para conhecer suas variações e as relações entre eles, através de um estudo descritivo e de correlação entre diferentes parâmetros, para comparar as condições de conforto térmico e os limites críticos estabelecidos para as aves. Com base nos resultados desta pesquisa, considerando os índices de entalpia obtidos através de médias históricas, pode-se concluir que a utilização de aquecimento para as aves na primeira e segunda semanas de alojamento, no período de inverno, considerando o local estudado, é imprescindível, devido às baixas temperaturas registadas. Para aves alojadas na terceira, quarta, quinta e sexta semana de idade, considerando os índices de entalpia, pode-se concluir que é necessária a utilização de sistemas de resfriamento para as aves. corte ar forma objetivouse objetivou se Londrina Paraná utilizaramse utilizaram 196 202 INMET, INMET , (INMET) Brasília Federal eles parâmetros pesquisa históricas podese pode alojamento inverno estudado imprescindível registadas terceira quarta idade 19 20 (INMET 1 2