Abstract Introduction: Macrobrachium amazonicum is widely distributed in South America, occurring in the Orinoco, Amazon and Paraguay river basins, being used as an important source of protein for feeding riverside populations. This prawn is host to the crustacean ectoparasites Probopyrus pandalicola, Probopyrus floridensis and Probopyrus bithynis (Bopyridae) that infest the gill cavity of the species. Objective: To report the prevalence and abundance of ectopasites (P. pandalicola, P. floridensis and P. bithynis) and the condition factor in M. amazonicum from the Amazon River, Eastern Amazon. Methods: The prawns were collected from May 2017 to April 2018, with the artisanal trap matapi in the municipalities of Mazagão (state of Amapá) and Santarém (state of Pará), Brazil. Results: 216 prawns infected with ectoparasites were captured, 133 in the Mazagão region (53 P. pandalicola, 48 P. bithynis and 32 P. floridensis) and 83 in the Santarém region (45 P. pandalicola and 38 P. bithynis). In Mazagão, the highest prevalence values were recorded in May (1.98 %) for P. floridensis, July (2.18 %) for P. bithynis, and March (1.93 %) for P. pandalicola. In the Santarém region, the highest prevalence values were recorded in February, 3.47 for P. pandalicola and 2.89 for P. bithynis. Regarding the abundance of parasites, there was no difference between the species grouped between the regions (t = 0.32, p = 0.739). Parasitized prawns from Mazagão and Santarém presented condition factors below expectations, with an average of 0.85 ± 0.55 for Santarém and 0.88 ± 0.44 for Mazagão. Conclusions: During the study it was found that P. pandalicola, P. bithynis and P. floridensis were present in M. amazonicum throughout the year. Furthermore, there was an effect of parasitism on M. amazonicum in the study areas.

Resumen Introducción: Macrobrachium amazonicum está ampliamente distribuido en América del Sur, se encuentra en las cuencas de los ríos Orinoco, Amazonas y Paraguay, se utiliza como una fuente importante de proteínas para alimentar a las poblaciones ribereñas. Este camarón es hospedero de los ectoparásitos crustáceos: Probopyrus pandalicola, Probopyrus floridensis y Probopyrus bithynis (Bopyridae), que infestan la cavidad branquial de la especie. Objetivo: Reportar la prevalencia y abundancia de ectoparásitos (P. pandalicola, P. floridensis y P. bithynis) y el factor de condición de M. amazonicum en el| río Amazonas, al este de la Amazonía. Métodos: Los camarones fueron recolectados entre mayo 2017 y abril 2018, con la ayuda de matapis (trampa artesanal) en los municipios de Mazagão (Estado de Amapá) y Santarém (Estado de Pará), Brasil. Resultados: Se capturaron un total de 216 camarones parasitados con ectoparásitos, 133 de ellos en la región de Mazagão (53 P. pandalicola, 48 P. bithynis y 32 P. floridensis) y 83 en la región de Santarém (45 P. pandalicola y 38 P. bithynis). En Mazagão, los valores más altos de prevalencia se registraron en mayo (1.98 %) para P. floridensis, julio (2.18 %) para P. bithynis y marzo (1.93 %) para P. pandalicola. En la región de Santarém, los valores más altos de prevalencia se registraron en febrero, de 3.47 para P. pandalicola y 2.89 para P. bithynis. En cuanto a la abundancia de parásitos, no hubo diferencia entre las especies agrupadas entre las regiones (t = 0.32, p = 0.739). Los camarones parasitados de Mazagão y Santarém mostraron factores de condición por debajo de lo esperado, con un promedio de 0.85 ± 0.55 para Santarém y 0.88 ± 0.44 para Mazagão. Conclusiones: Durante el estudio, se encontró que P. pandalicola, P. bithynis y P. floridensis estuvieron presentes en M. Amazonicum todo el año. Además, hubo un efecto de parasitismo sobre M. Amazonicum en las áreas de estudio.

Abstract Background: Hymenoptera stings can produce IgE-mediated reactions, toxic reactions, or atypical reactions, which are rare. Cold urticaria has been described among the cutaneous manifestations in the atypical ones, but there is only one case of chronic urticaria. Case report: A 56-year-old female patient experienced palmoplantar pruritus and generalized urticaria 60 minutes after two vespid stings, requiring medical assistance and several cycles of home treatment with oral antihistamines and corticosteroids for resolution in 12 weeks. Allergological studies showed normal tryptase and primary sensitization to Polistes dominula venom. Given the patient's profession, venom immunotherapy was started with Polistes dominula 100% without recurrence of urticaria after its administration. Conclusion: We present a case of IgE-mediated systemic reaction followed by self-limited chronic urticaria, related chronologically to the same vespid sting trigger.

Resumen Antecedentes: La picadura de himenópteros puede producir reacciones mediadas por IgE, reacciones tóxicas o reacciones atípicas poco frecuentes. Dentro de las manifestaciones cutáneas por reacciones atípicas se incluye urticaria por frío, y sólo existe un informe de caso de urticaria crónica. Reporte de caso: Paciente femenina de 56 años, quien 60 minutos después de recibir dos picaduras de véspidos manifestó prurito palmoplantar y urticaria generalizada, precisando asistencia sanitaria y varios ciclos de tratamiento domiciliario con antihistamínicos y corticosteroides por vía oral, con curación completa luego de 12 semanas. El estudio alergológico reportó: concentración de triptasa normal y sensibilización primaria al veneno de Polistes dominula. Debido a la profesión de la paciente se inició inmunoterapia con veneno de Polistes dominula al 100%, sin recurrencia de la urticaria después de la administración. Conclusión: El caso aquí expuesto combina una reacción sistémica mediada por IgE seguida de urticaria crónica, de curso autolimitado, cronológicamente relacionada con el mismo desencadenante (picadura de véspidos).

RESUMEN En la Amazonía ecuatoriana, cada día son mayores los desafíos para la identificación de especies fitorremediadoras que disminuyan la contaminación por metales pesados producto de la actividad minera. El objetivo de este trabajo fue evaluar el potencial bioacumulador de metales pesados de tres especies forestales de rápido crecimiento (Ochroma pyramidale, Piptocoma discolor y Bambusa vulgaris) en un área de extracción minera, en la comunidad Yutzupino, Napo para la restauración. Se recolectaron muestras de hoja y suelo para determinar la concentración de metales pesados (Cd, Pb, Fe y Ni), mediante espectrometría de absorción atómica y se calculó el factor de bioconcentración (FBC). Los análisis foliares mostraron que B. vulgaris presentó mayor concentración de Ni (6,89 mg kg-1) y Fe (156,33 mg kg-1) y P. discolor contenía Cd (0,35 mg kg-1) y Pb (55,67 mg kg -1). Los análisis de suelos mostraron un patrón de variación en B. vulgaris superior en Cd, Ni y Pb y en O. pyramidale para Fe. El factor de bioconcentración permitió identificar la capacidad acumuladora de las especies, lo que sugiere que P. discolor puede ser utilizada como especie fitorremediadora para sitios contaminadas por Cd y Pb, O. pyramidale para Ni y B. vulgaris para Fe.

RESUMO Na Amazônia equatoriana, os desafios para a identificação de espécies de fitorremediação para reduzir a contaminação por metais pesados provenientes de atividades de mineração aumentam a cada dia. O objetivo deste trabalho foi avaliar o potencial bioacumulativo de metais pesados de três espécies florestais de crescimento rápido (Ochroma pyramidale, Piptocoma discolor e Bambusa vulgaris) em uma área de extração de mineração na comunidade de Yutzupino, Napo, para restauração. Amostras de folhas e de solo foram coletadas para determinar a concentração de metais pesados (Cd, Pb, Fe e Ni) por espectrometria de absorção atômica e o fator de bioconcentração (BCF) foi calculado. A análise das folhas mostrou que a B. vulgaris tinha concentrações mais altas de Ni (6,89 mg kg-1) e Fe (156,33 mg kg-1) e a P. discolor continha Cd (0,35 mg kg-1) e Pb (55,67 mg kg -1). As análises do solo mostraram um padrão de variação em B. vulgaris maior em Cd, Ni e Pb e em O. pyramidale para Fe. O fator de bioconcentração identificou a capacidade de acumulação das espécies, sugerindo que P. discolor pode ser usada como uma espécie de fitorremediação para locais contaminados com Cd e Pb, O. pyramidale para Ni e B. vulgaris para Fe.

ABSTRACT In the Ecuadorian Amazon, the challenges for identifying, phytoremediation species that reduce heavy metal contamination resulting from mining activity are increasing every day. The objective of this work was to evaluate the bioaccumulator potential of heavy metals of three fast-growing forest species (Ochroma pyramidale, Piptocoma discolor and Bambusa vulgaris) in a mining extraction area, in the Yutzupino community, Napo for restoration. Leaf and soil samples were collected to determine the concentration of heavy metals (Cd, Pb, Fe and Ni) using atomic absorption spectrometry and the bioconcentration factor (BCF) was calculated. Leaf analyzes showed that B. vulgaris presented a higher concentration of Ni (6.89 mg kg-1 ) and Fe (156.33 mg kg-1 ) and P. discolor contained Cd (0.35 mg kg-1 ) and Pb (55.67 mg kg -1 ). Soil analyzes showed a pattern of variation in B. vulgaris superior in Cd, Ni and Pb and in O. pyramidale for Fe. The bioconcentration factor allowed identifying the accumulating capacity of the species, which suggests that P. discolor may be used as a phytoremediation species for sites contaminated by Cd and Pb, O. pyramidale for Ni and B. vulgaris for Fe.

ABSTRACT The purpose of this study was to report the ocular changes observed after a bee sting with a stinger retained in the cornea. We show the treatment and outcome of an unusual corneal injury and its pathogenesis. This is a case report and literature review of ocular injuries caused by bee stings. A 63-year-old female patient sought emergency ophthalmic care because of a bee sting on the cornea of her right eye six days before. She complained of blurred vision, pain, and ocular hyperemia. She had glare sensitivity on visual acuity in the affected eye. Examination revealed moderate hyperemia of the bulbar conjunctiva, corneal edema with Descemet’s folds and a stinger lodged in the temporal region, in the deep stroma of the cornea. The patient was admitted to the operating room under general anesthesia. During surgery, the stinger had to be removed via the anterior chamber, by performing a paracentesis and washing the anterior chamber with a double flushing and balanced saline solution. There is still no standard treatment in the literature for patients with eye injuries caused by bee stings, and early identification and recognition of possible sight-threatening complications is important. pathogenesis stings 63yearold yearold 63 year old before vision pain conjunctiva Descemets Descemet s region anesthesia surgery solution sightthreatening sight threatening important 6

RESUMO O propósito deste estudo foi reportar as alterações oculares observadas após picada de abelha com ferrão retido na córnea. Destacamos o tratamento e o desfecho de uma lesão de córnea incomum e sua patogênese. Trata-se de relato de caso e revisão da literatura de lesões oculares por picada de abelha. Paciente do sexo feminino, 63 anos, procurou atendimento oftalmológico de urgência devido à picada de abelha na córnea do olho direito há 6 dias. Queixava-se de embaçamento visual, dor e hiperemia ocular. Apresentou acuidade visual de vultos no olho afetado. Ao exame, notaram-se hiperemia moderada de conjuntiva bulbar, edema corneano com dobras de Descemet e presença do ferrão alojado na região temporal, no estroma profundo da córnea. A paciente foi internada para ser abordada no centro cirúrgico sob anestesia geral. Durante a cirurgia, o ferrão teve que ser retirado via câmara anterior, mediante a realização de uma paracentese e uma lavagem da câmara anterior, com dupla via e solução salina balanceada. Ainda não existe na literatura um tratamento padrão na abordagem de pacientes com lesões oculares por picada de abelha, sendo importantes a identificação e o reconhecimento precoce de possíveis complicações que ameacem a visão. patogênese Tratase Trata se feminino anos dias Queixavase Queixava ocular afetado exame notaramse notaram bulbar temporal geral cirurgia anterior balanceada visão

Resumo O camarão-da-amazônia ou Macrobrachium amazonicum (Heller, 1862) é amplamente distribuído na América do Sul, ocorrendo nos rios Orinoco e Amazonas, importante fonte renda de familias riberinhas. Esse camarão é hospedeiro de ectoparasitas crustáceos do gênero Probopyrus (Giard & Bonnier, 1888) (Bopyridae) que infestam a cavidade branquial da espécie. O presente estudo tem o objetivo de relatar novas ocorrências de ectopasitas do gênero Probopyrus no camarão-da-amazônia, capturados no rio Amazonas. Macrobrachium amazonicum foram coletados no período de maio de 2017 e abril de 2018, e novamente de julho de 2021 a maio de 2022, nas regiões da Ilha de Santana e Rio Mazagão, estado do Amapá, Brasil. Entre 5.179 exemplares de camarões capturados, 133 foram parasitados sendo quatro pelos ectoparasitas Probopyrus pandalicola (Packard, 1879), Probopyrus bithynis (Richardson, 1904), Probopyrus floridensis (Richardson, 1904) e Probopyrus palaemoni (Lemos de Castro & Brasil Lima, 1974). Registrado pela primeira vez a ocorrência no M. amazonicum por P. floridensis e P. palaemoni no litoral norte do Brasil. Esses quatro ectoparasitos não se limitam a espécies ou gêneros hospedeiros específicos, observados nesse estudo que relata quatro espécies de Probopyrus infestando M. amazonicum. camarãodaamazônia amazônia Heller, Heller (Heller 1862 Sul Amazonas riberinhas Giard Bonnier 1888 Bopyridae (Bopyridae espécie camarãodaamazônia, amazônia, 201 2018 202 2022 Mazagão Amapá 5179 5 179 5.17 13 Packard, Packard (Packard 1879, 1879 , 1879) Richardson, Richardson (Richardson 1904, 1904 Lemos Lima 1974. 1974 . 1974) M P específicos 186 188 20 517 17 5.1 1 187 190 197 18 2 51 5. 19

Abstract The Amazon prawn or Macrobrachium amazonicum (Heller, 1862) is widely distributed in South America, occurring in the Orinoco and Amazon rivers, and forms an important source of income for riverside families. This prawn hosts crustacean ectoparasites of the genus Probopyrus (Giard & Bonnier, 1888) (Bopyridae) that infest its gill cavity. The aim of the present study was to report new occurrences of Probopyrus in Amazon prawns caught in the Amazon River. Macrobrachium amazonicum prawns were collected between May 2017 and April 2018, and again from July 2021 to May 2022 in the regions of Ilha de Santana and Rio Mazagão, state of Amapá, Brazil. Among the 5,179 prawn specimens caught, 133 were parasitized by the ectoparasites Probopyrus pandalicola (Packard, 1879), Probopyrus bithynis (Richardson, 1904), Probopyrus floridensis (Richardson, 1904) and Probopyrus palaemoni (Lemos de Castro & Brasil Lima, 1974). These occurrences of P. floridensis and P. palaemoni in M. amazonicum were the first records of this on the northern coast of Brazil. These four ectoparasites are not limited to specific host species or genera, as observed in this study, which reports four species of Probopyrus infesting M. amazonicum. Heller, Heller (Heller 1862 America rivers families Giard Bonnier 1888 Bopyridae (Bopyridae cavity River 201 2018 202 Mazagão Amapá Brazil 5179 5 179 5,17 13 Packard, Packard (Packard 1879, 1879 , 1879) Richardson, Richardson (Richardson 1904, 1904 Lemos Lima 1974. 1974 . 1974) P M genera 186 188 20 517 17 5,1 1 187 190 197 18 2 51 5, 19

Resumen Objetivo: describir la completitud de las notificaciones de envenenamiento por serpientes, arañas y escorpiones en el Sistema de Información de Enfermedades de Declaración - Sinan, en Brasil y regiones, de 2007 a 2019. Métodos: se analizaron campos esenciales y no obligatorios para las notificaciones de ofidismo, aracneismo y escorpionismo, considerando las categorías de completitud: Excelente (≤5,0% de incompletitud), Buena (5,0% a 10,0%), Regular (10,0% a 20,0%), Mala (20,0% y ≤50,0%) y Muy mala (>50,0%). Se estimó el cambio proporcional en la completitud entre 2007-2019. Resultados: se investigaron 1.871.462 notificaciones. Los campos “manifestaciones locales y sistémicas”, “zona de ocurrencia”, “clasificación y evolución de casos” presentaron excelente o buena completitud. “Escolaridad” y “raza/color de piel” regular o mala completitud. “Ocupación” fue predominantemente mala/muy mala completitud. “Zona”,“evolución” y “escolaridad” mostraron empeoramiento proporcional en la completitud en la mayoría de las regiones (VP<0). Conclusión: hay mejoría y completitud en la mayoría de los campos, aunque los campos socioeconómico y ocupacional demandan atención. Objetivo serpientes Sinan 200 2019 Métodos ofidismo escorpionismo ≤5,0% 50 5 0 (≤5,0 incompletitud, incompletitud , incompletitud) 5,0% (5,0 10,0%, 100 10,0% 10 10,0%) (10,0 20,0%, 20,0% 20 20,0%) (20,0 ≤50,0% 500 >50,0%. >50,0% . (>50,0%) 20072019. 20072019 2007-2019 Resultados 1871462 1 871 462 1.871.46 manifestaciones sistémicas, sistémicas sistémicas” zona ocurrencia, ocurrencia ocurrencia” clasificación casos Escolaridad “Escolaridad raza/color razacolor raza color piel Ocupación “Ocupación malamuy muy Zona,evolución Zonaevolución Zona “Zona”,“evolución escolaridad “escolaridad VP<0. VP0 VP VP<0 (VP<0) Conclusión atención 201 ≤5,0 (≤5, 5,0 (5, 10,0 (10, 20,0 2 (20, ≤50,0 >50,0 (>50,0% 2007201 2007-201 187146 87 46 1.871.4 VP< (VP<0 ≤5, (≤5 5, (5 10, (10 20, (20 ≤50, >50, (>50,0 200720 2007-20 18714 8 4 1.871. (VP< ≤5 (≤ ( (1 (2 ≤50 >50 (>50, 20072 2007-2 1871 1.871 (VP ≤ >5 (>50 2007- 187 1.87 > (>5 18 1.8 (> 1.

Abstract Objective: to describe the completeness of notifications of accidents involving venomous animals held on the Notifiable Health Conditions Information System (SINAN), in Brazil and its macro-regions, from 2007 to 2019. Methods: we analyzed essential and non-mandatory fields for snakebite, spider bite and scorpion sting notifications, considering the following completeness categories: Excellent (≤5.0% incompleteness), Good (5.0% to 10.0%), Regular (10.0% to 20.0%), Poor (20.0% to ≤50.0%) and Very Poor (>50.0%). Proportional change in completeness between 2007 and 2019 was estimated. Results: 1,871,462 notifications were investigated. The “localized manifestations”, “systemic manifestations”, “case classification”, “case progression” and “zone of occurrence” fields had excellent or good completeness. Completeness was regular or poor for the “schooling” and “race/color” fields. The “occupation” field was predominantly poorly or very poorly filled in. There was a proportional worsening in completeness (PC<0) in most regions for the “zone of occurrence”, “case progression” and “schooling” fields. Conclusion: completeness of most fields improved, although socioeconomic and occupational fields require more attention. Objective SINAN, SINAN , (SINAN) macroregions, macroregions macro regions, macro-regions 200 Methods nonmandatory non mandatory snakebite categories ≤5.0% 50 5 0 (≤5.0 incompleteness, incompleteness incompleteness) 5.0% (5.0 10.0%, 100 10.0% 10 10.0%) (10.0 20.0%, 20.0% 20 20.0%) (20.0 ≤50.0% 500 >50.0%. >50.0% . (>50.0%) 201 estimated Results 1871462 1 871 462 1,871,46 investigated localized manifestations, manifestations manifestations” systemic case classification, classification classification” progression zone occurrence schooling “schooling race/color racecolor race color “race/color occupation “occupation PC<0 PC0 PC (PC<0 occurrence, Conclusion improved attention (SINAN ≤5.0 (≤5. 5.0 (5. 10.0 (10. 20.0 2 (20. ≤50.0 >50.0 (>50.0% 187146 87 46 1,871,4 PC< (PC< ≤5. (≤5 5. (5 10. (10 20. (20 ≤50. >50. (>50.0 18714 8 4 1,871, (PC ≤5 (≤ ( (1 (2 ≤50 >50 (>50. 1871 1,871 ≤ >5 (>50 187 1,87 > (>5 18 1,8 (> 1,

Resumo Objetivo: descrever a completude das notificações de acidentes causados por animais peçonhentos no Sistema de Informação de Agravos de Notificação, no Brasil e macrorregiões, em 2007-2019. Métodos: analisou-se a completude de campos de preenchimento essenciais e não obrigatórios das notificações de ofidismo, araneísmo e escorpionismo, utilizando-se a classificação de “Excelente” (≤ 5,0% de incompletude), “Bom” (5,0% a 10,0%), “Regular” (10,0% a 20,0%), “Ruim” (20,0% e ≤ 50,0%) e “Muito ruim” (> 50,0%); estimou- -se a variação proporcional (VP) da completude. Resultados: foram investigadas 1.871.462 notificações; os campos “manifestações locais”, “sistêmicas”, “classificação do caso”, “evolução do caso” e “zona de ocorrência” apresentaram completude excelente ou boa; “escolaridade” e “raça/cor da pele”, completude regular ou ruim; ocupação, completude ruim ou muito ruim; “zona”, “evolução do caso” e “escolaridade” apresentaram piora proporcional na completude, na maioria das regiões (VP < 0). Conclusão: melhorou a completude na maioria dos campos, exceto socioeconômicos e ocupacionais, que demandam maior atenção. Objetivo Notificação macrorregiões 20072019. 20072019 2007 2019. 2019 2007-2019 Métodos analisouse analisou se ofidismo escorpionismo utilizandose utilizando Excelente “Excelente ( 50 5 0 5,0 incompletude, incompletude , incompletude) Bom “Bom (5,0 10,0%, 100 10,0% 10 10,0%) Regular “Regular (10,0 20,0%, 200 20,0% 20 20,0%) Ruim “Ruim (20,0 50,0% 500 Muito > estimou VP Resultados 1871462 1 871 462 1.871.46 manifestações locais, locais locais” sistêmicas, sistêmicas “sistêmicas” caso, caso evolução zona ocorrência boa escolaridade “escolaridade raça/cor raçacor raça cor pele, pele pele” ocupação zona, “zona” 0. . 0) Conclusão ocupacionais atenção 2007201 201 2007-201 5, (5, 10,0 (10, 20,0 2 (20, 50,0 187146 87 46 1.871.4 “sistêmicas 200720 2007-20 (5 10, (10 20, (20 50, 18714 8 4 1.871. 20072 2007-2 (1 (2 1871 1.871 2007- 187 1.87 18 1.8 1.

Abstract Objective: to describe the completeness of notifications of accidents involving venomous animals held on the Notifiable Health Conditions Information System (SINAN), in Brazil and its macro-regions, from 2007 to 2019. Methods: we analyzed essential and non-mandatory fields for snakebite, spider bite and scorpion sting notifications, considering the following completeness categories: Excellent (≤5.0% incompleteness), Good (5.0% to 10.0%), Regular (10.0% to 20.0%), Poor (20.0% to ≤50.0%) and Very Poor (>50.0%). Proportional change in completeness between 2007 and 2019 was estimated. Results: 1,871,462 notifications were investigated. The “localized manifestations”, “systemic manifestations”, “case classification”, “case progression” and “zone of occurrence” fields had excellent or good completeness. Completeness was regular or poor for the “schooling” and “race/color” fields. The “occupation” field was predominantly poorly or very poorly filled in. There was a proportional worsening in completeness (PC<0) in most regions for the “zone of occurrence”, “case progression” and “schooling” fields. Conclusion: completeness of most fields improved, although socioeconomic and occupational fields require more attention.

Resumen Objetivo: describir la completitud de las notificaciones de envenenamiento por serpientes, arañas y escorpiones en el Sistema de Información de Enfermedades de Declaración - Sinan, en Brasil y regiones, de 2007 a 2019. Métodos: se analizaron campos esenciales y no obligatorios para las notificaciones de ofidismo, aracneismo y escorpionismo, considerando las categorías de completitud: Excelente (≤5,0% de incompletitud), Buena (5,0% a 10,0%), Regular (10,0% a 20,0%), Mala (20,0% y ≤50,0%) y Muy mala (>50,0%). Se estimó el cambio proporcional en la completitud entre 2007-2019. Resultados: se investigaron 1.871.462 notificaciones. Los campos “manifestaciones locales y sistémicas”, “zona de ocurrencia”, “clasificación y evolución de casos” presentaron excelente o buena completitud. “Escolaridad” y “raza/color de piel” regular o mala completitud. “Ocupación” fue predominantemente mala/muy mala completitud. “Zona”,“evolución” y “escolaridad” mostraron empeoramiento proporcional en la completitud en la mayoría de las regiones (VP<0). Conclusión: hay mejoría y completitud en la mayoría de los campos, aunque los campos socioeconómico y ocupacional demandan atención.

Resumo Objetivo: descrever a completude das notificações de acidentes causados por animais peçonhentos no Sistema de Informação de Agravos de Notificação, no Brasil e macrorregiões, em 2007-2019. Métodos: analisou-se a completude de campos de preenchimento essenciais e não obrigatórios das notificações de ofidismo, araneísmo e escorpionismo, utilizando-se a classificação de “Excelente” (≤ 5,0% de incompletude), “Bom” (5,0% a 10,0%), “Regular” (10,0% a 20,0%), “Ruim” (20,0% e ≤ 50,0%) e “Muito ruim” (> 50,0%); estimou- -se a variação proporcional (VP) da completude. Resultados: foram investigadas 1.871.462 notificações; os campos “manifestações locais”, “sistêmicas”, “classificação do caso”, “evolução do caso” e “zona de ocorrência” apresentaram completude excelente ou boa; “escolaridade” e “raça/cor da pele”, completude regular ou ruim; ocupação, completude ruim ou muito ruim; “zona”, “evolução do caso” e “escolaridade” apresentaram piora proporcional na completude, na maioria das regiões (VP < 0). Conclusão: melhorou a completude na maioria dos campos, exceto socioeconômicos e ocupacionais, que demandam maior atenção.

Abstract Brazil is located between the Equator and Tropic of Capricorn, which allows diverse climates, reliefs, and habitats for arthropods, which sting represents a risk to human health and a public health issue. This manuscript updates the epidemiological data of cases of human envenoming by spiders, scorpions, and insects with medical relevance in Brazil from 2010 to 2021. Epidemiological data were taken using the Brazilian Notifiable Diseases Information System. Statistics of non-parametric data used the Kruskal-Wallis followed by the Nemenyi test. On average, more than 145,000 envenomation and 145 deaths are recorded annually, and more than 60% of deaths are caused by scorpion bites. When the number of deaths was pondered by the number of cases with each arthropod, bees kill the most. Most stings cause mild symptoms and affect men of working age. The incidence decreases during the colder months, which is better noticeable in regions with well-defined seasons. The distribution is distinct among the regions: Southeast, Northeast, and South have the highest rate of bites. The growing number of cases of envenomation reported annually is a serious public health concern, especially involving scorpions, and highlights the importance of studying arthropod venom and improving the therapies. Capricorn climates reliefs arthropods issue spiders scorpions 201 2021 System nonparametric non parametric KruskalWallis Kruskal Wallis test average 145000 000 145,00 14 60 bites most age months welldefined well defined seasons Southeast Northeast concern therapies 20 202 14500 00 145,0 1 6 2 1450 0 145,

Abstract Learning to anticipate events based on the predictive relationship between an action and an outcome (operant conditioning) is a form of associative learning shared by humans and most of other living beings, including invertebrates. Several behavioral studies on the mechanisms of operant conditioning have included Melipona quadrifasciata, a honey bee that is easily manipulated due to lack of sting. In this work, brain proteomes of Melipona bees trained using operant conditioning and untrained (control) bees were compared by two-dimensional gel electrophoresis analysis within pI range of 3-10 and 4–7; in order to find proteins specifically related to this type of associative learning.One protein was detected with differential protein abundance in the brains of trained bees, when compared to not trained ones, through computational gel imaging and statistical analysis. This protein was identified by peptide mass fingerprinting and MS/MS peptide fragmentation using a MALDI-TOF/TOF mass spectrometer as one isoform of arginine kinase monomer, apparently dephosphorylated. Brain protein maps were obtained by 2-DE (Two-dimensional gel electrophoresis) from a total proteins and phosphoproteins extract of the bee Melipona quadrifasciata. One isoform of arginine kinase, probably a dephosphorylated isoform, was significantly more abundant in the brain of trained bees using operant conditioning. Arginine kinase has been reported as an important enzyme of the energy releasing process in the visual system of the bee, but it may carry out additional and unexpected functions in the bee brain for learning process. beings invertebrates quadrifasciata sting work control (control twodimensional two dimensional 310 3 10 3-1 4–7 47 4 7 learningOne ones MSMS MS MALDITOF/TOF MALDITOFTOF MALDI TOF/TOF TOF monomer 2DE DE 2 Twodimensional Two 31 1 3- 4– MALDITOF TOFTOF

RESUMEN Objetivo: Caracterizar los accidentes de escorpiones en el Centro de Información y Asistencia Toxicológica (CIATox) de Campinas, analizar los factores de riesgo relacionados con la clasificación moderada y severa y determinar el grupo etario de mayor riesgo para esta clasificación. Método: Estudio transversal y retrospectivo, con pacientes atendidos presencialmente por el CIATox, que sufrieron un accidente de escorpión, desde enero de 2015 a diciembre de 2019. Se realizó un análisis descriptivo e inferencial. Para la variable edad se construyó una curva ROC con el fin de determinar puntos de corte en relación a la clasificación de severidad. Los modelos de regresión de Poisson se ajustaron considerando la clasificación de severidad como variable dependiente. Resultados: Se analizaron 754 casos con edad media de 36,05 años, en su mayoría mujeres y accidentes no laborales ocurridos en la zona urbana. El escorpión más frecuente fue el Tityus serrulatus. Los factores de riesgo encontrados para mayor gravedad fueron el grupo etario hasta 22 años y la atención previa en otros servicios de salud. Conclusión: El grupo etario hasta los 22 años debe ser utilizado como factor predictivo de severidad en la evaluación clínica de los pacientes picados por escorpiones para realizar un manejo adecuado de los casos. Objetivo CIATox (CIATox Campinas Método retrospectivo 201 2019 inferencial dependiente Resultados 75 3605 36 05 36,0 urbana serrulatus 2 salud Conclusión 20 7 360 3 0 36,

ABSTRACT Objective: To characterize scorpion accidents at the Information and Toxicological Assistance Center (CIATox) in Campinas, to analyze risk factors related to the moderate and severe classification, and to determine the age group at greatest risk for this classification. Method: Cross-sectional and retrospective study, with patients assisted in person at CIATox, who had a scorpion accident, from January 2015 to December 2019. Descriptive and inferential analysis was conducted. For the age variable, a ROC curve was constructed to determine cutoff points in relation to the severity classification. Poisson regression models were adjusted considering severity classification as the dependent variable. Results: A total of 754 cases with a mean age of 36.05 years, mostly female and non-occupational accidents that occurred in the urban area, was analyzed. The most frequent scorpion was the Tityus serrulatus. The risk factors found for greater severity were age group up to 22 years and previous care in other health services. Conclusion: The age range up to 22 years old should be used as a predictive factor of severity in the clinical evaluation of patients stung by scorpions to carry out adequate management of cases. Objective CIATox (CIATox Campinas Method Crosssectional Cross sectional study accident 201 2019 conducted variable Results 75 3605 36 05 36.0 nonoccupational non occupational area analyzed serrulatus 2 services Conclusion 20 7 360 3 0 36.

RESUMO Objetivo: Caracterizar os acidentes escorpiônicos do Centro de Informação e Assistência Toxicológica (CIATox) de Campinas, analisar fatores de risco relacionados à classificação moderada e grave e determinar faixa etária de maior risco para esta classificação. Método: Estudo transversal e retrospectivo, com pacientes atendidos presencialmente pelo CIATox, que sofreram acidente escorpiônico, de janeiro de 2015 a dezembro de 2019. Foi realizada análise descritiva e inferencial. Para a variável idade foi construída uma curva ROC com o intuito de determinar pontos de corte em relação à classificação de gravidade. Foram ajustados modelos de regressão Poisson considerando a classificação de gravidade como variável dependente. Resultados: Foram analisados 754 casos com idade média de 36,05 anos, maioria do sexo feminino e acidentes não ocupacionais ocorridos na zona urbana. O escorpião mais frequente foi o Tityus serrulatus. Os fatores de risco encontrados para maior gravidade foram faixa etária até 22 anos e atendimento prévio em outros serviços de saúde. Conclusão: A faixa etária até 22 anos deve ser usada como fator preditivo de gravidade na avaliação clínica dos pacientes picados por escorpião para realização de manejo adequado dos casos. Objetivo CIATox (CIATox Campinas Método retrospectivo escorpiônico 201 2019 inferencial dependente Resultados 75 3605 36 05 36,0 urbana serrulatus 2 saúde Conclusão 20 7 360 3 0 36,

ABSTRACT We report the first pediatric case of a Jaguajir agamemnon scorpion sting. This case occurred in the state of Paraná and is the first record of this species in southern Brazil. The patient was admitted within 15 min, with pain, local edema, erythema, whole-body pruritus, and tongue paresthesia within the first two hours, which disappeared thereafter. The patient’s condition was considered mild, with a positive outcome, and she recovered completely. sting Brazil 1 min pain edema erythema wholebody whole body pruritus hours thereafter patients s mild outcome completely

Abstract A 44-year-old man was admitted to the emergency department after a scorpion sting. He presented paresthesia, thoracoabdominal pain and nausea. From the moment of admission, he presented atypical chest pain, epigastric abdominal pain of mild intensity, associated with nausea. An ECG was taken that did not show alterations, troponin was elevated, he was admitted to intensive care unit, management was initiated, subsequently corroborating in the angiography, a type 1 coronary artery dissection in the intermediate ramus as the cause of coronary event. The patient received a vial of antiscorpionic serum in the first level of care, later treatment with beta-blocker, antiaggregation with acetylsalicylic acid and clopidogrel was indicated, with progressive reduction of his symptoms. He is an asymptomatic graduate. A case of type 2 acute myocardial infarction due to coronary dissection after a scorpion sting is reported, there are no previously reported cases in the world.

Resumen Hombre de 44 años que acude al servicio de urgencias posterior a picadura de escorpión. Presentaba parestesias, dolor toracoabdominal y náuseas. Desde el momento del ingreso presentó dolor torácico atípico, dolor abdominal epigástrico de intensidad leve, asociado a náuseas. Se realizó electrocardiograma que no evidenció alteraciones. La troponina fue elevada, por lo que se llevó a cuidado intensivo y se inició manejo antiisquémico, corroborando posteriormente en la angiografía una disección coronaria tipo 1 en el ramus intermedio como causante de su evento coronario. El paciente recibió un vial de suero antiescorpiónico en primer nivel de atención y posteriormente se indicó manejo con betabloqueante, antiagregación con ácido acetilsalicílico y clopidogrel, con disminución progresiva de sus síntomas. Es egresado asintomático. Se reporta un caso de infarto agudo de miocardio tipo 2 por disección coronaria posterior a una picadura de escorpión, del cual no hay casos reportados previamente en el mundo.

Abstract: The article analyzes an intense controversy in which prominent members of a conservative sector of the Aguascalientes society took part, who manifested themselves through the local press, regarding the fact that in the second courtyard of the Government Palace the Chilean artist Osvaldo Barra Cunningham made, in 1961, the mural painting Aguascalientes en la historia. Some subjects in which the historical evolution of the Catholic Church is represented caused sting in certain sectors of the population, who felt aggrieved for interpreting, that the faith, beliefs and religious sensibilities of Catholics were freely offended. Images of religion captured on the walls of a civic monument, in an atmosphere prior to the Second Vatican Council, is the object of study in this paper.

Resumen: El artículo analiza una intensa controversia en la que tomaron parte miembros prominentes de un sector conservador de la sociedad aguascalentense, quienes se manifestaron a través de la prensa local a propósito de la pintura mural Aguascalientes en la historia que el artista chileno Osvaldo Barra Cunningham realizó, en 1961, en el segundo patio del Palacio de Gobierno. Algunos temas en los que se representa el devenir histórico de la Iglesia católica causaron escozor en ciertos sectores de la población, quienes se sintieron agraviados, porque se ofendía gratuitamente la fe, creencias y sensibilidad religiosa de los católicos. Imágenes de lo religioso plasmadas en los muros de un monumento cívico, en una atmósfera previa al Concilio Vaticano II, son el objeto de estudio en este artículo.

Resumen La inmunoterapia con veneno de himenópteros (IVH) es, a largo plazo, un tratamiento eficaz para evitar nuevas reacciones sistémicas en pacientes con alergia a este tipo de insectos. La prueba de repicadura controlada es el estudio de referencia para confirmar la tolerancia del individuo. Sin embargo, no se ha generalizado su indicación clínica, por lo que la prueba de activación de basófilos (TAB) resulta una buena alternativa, pues valora de manera funcional la respuesta al alérgeno y está exenta de los riesgos asociados con la provocación. En esta revisión se explora la utilidad de la TAB en el seguimiento y valoración del éxito de la IVH. Se seleccionaron estudios que evalúan los cambios entre una TAB basal y en otro momento de la fase de inicio o mantenimiento de la IVH. Se incluyeron 10 estudios con datos de 167 pacientes, de los que el 29% había tenido prueba de repicadura controlada. Para vigilar la eficacia de la IVH debe explorarse la respuesta del basófilo, con la determinación de las concentraciones submáximas del alérgeno, que reflejan la sensibilidad del basófilo. Los cambios en la respuesta máxima (reactividad) no pueden aportar información del estado de tolerancia, especialmente en las fases iniciales de la IVH.

Abstract Hymenoptera venom immunotherapy (HVI) is a long-term effective treatment to avoid new systemic reactions in patients with Hymenoptera allergy. The sting challenge test is considered the gold standard to confirm the tolerance. However, the use of this technique is not generalized in clinical practice, being the basophil activation test (BAT), which functionally explores allergen response, an alternative that does not entail any of the provocation risks associated with the sting challenge test. This study reviews the publications that used the BAT to follow up and evaluate the success of the HVI. Studies assessing the changes between a baseline BAT before the start and BATs performed between the starting and maintenance phases of the HVI were selected. Ten articles were found, comprising information from 167 patients, of which 29% used the sting challenge test. The studies concluded the importance of evaluating the responses with submaximal allergen concentrations, which reflect basophil sensitivity, to monitor the HVI using the BAT. It was also observed that changes in the maximum response (reactivity) could not reflect the clinical status of tolerance, particularly in the initial phases of HVI.