This study aims to propose a new method for derivatizing fatty acids (FAs) from human milk (HM), eliminating the lipid extraction step, and simplifying the preparation for gas chromatography with a flame ionization detection (GC-FID) analysis to quantify the FAs. The Design Expert software optimized the reaction times, concentrations, and sample amount. The proposed method (PM) was validated for lyophilized HM and results for the figures of merit for precision in relative standard deviation (RSD) (RSDintra-day 1.34-4.03% and RSDinter day 2.08 5.16%), accuracy (99.87-102.16%), and robustness are within a linear range of 3 to 38% lipids in HM samples. The atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) technique confirmed the efficiency of PM by expressing the molecular composition of triacylglycerol formed by FAs from the GC-FID technique. The PM requires a small sample size and conducts derivatization directly in the sample matrix, minimizing extraction errors. (FAs HM, , (HM) step GCFID GC FID (GC-FID times concentrations amount (PM RSD (RSD RSDintraday RSDintra 1.344.03% 134403 1.34 4.03% 1 34 4 03 1.34-4.03 208 2 08 2.0 5.16%, 516 5.16% 5 16 5.16%) 99.87102.16%, 998710216 99.87 102.16% 99 87 102 (99.87-102.16%) 38 samples ASAPMS/MS ASAPMSMS ASAP MS/MS MS (ASAP-MS/MS matrix errors (HM 344 1.344.03 13440 134 1.3 403 4.03 0 1.34-4.0 20 2. 51 5.16 87102 99.87102.16% 99871021 9987 99.8 10216 102.16 9 8 10 (99.87-102.16% ASAPMS MSMS 1.344.0 1344 13 1. 40 4.0 1.34-4. 5.1 8710 99.87102.16 9987102 998 99. 1021 102.1 (99.87-102.16 1.344. 4. 1.34-4 5. 871 99.87102.1 998710 102. (99.87-102.1 1.344 1.34- 99.87102. 99871 (99.87-102. 99.87102 (99.87-102 99.8710 (99.87-10 99.871 (99.87-1 (99.87- (99.87 (99.8 (99. (99 (9 (

The acetalization or transacetalization reactions produce acetals or ketals used in cosmetics, food, pharmaceuticals, and fuel additives. Besides synthesizing several products, the acetalization or transacetalization reactions can protect carbonyl groups, 1,2 and 1,3-diols in multi-step synthesis. This work studies the transacetalization of glycerol with 2,2-dimethoxypropane (2,2-DMP) to yield 2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane (solketal) by catalyzing with USY, HBeta, and HZSM-5 zeolites under cleaner and mild reaction conditions (reaction temperature: 25 °C; 2,2-DMP/glycerol molar ratio: 1; reaction time: 1 h; and catalyst concentration: 0.05-5 wt.%). For catalyst concentration equal to or greater than 2,5 wt.%, glycerol conversion stabilized near 96-97% for the three zeolites tested. The product selectivities also assume a constant value, close to 97% for solketal. For the lowest concentration of catalyst, the influence of the zeolite properties on the glycerol conversion, such as acidity and the strong/weak acid site ratio, was observed. Concerning the selectivity of the products, the results suggested that it can be based on zeolite topology, active sites at the particle surface, and the relative amount of micro and mesoporous HBeta, the most selective catalyst for acetals production, was reused at least once without loss of activity and selectivity. cosmetics food pharmaceuticals additives products groups 12 2 1, 1,3diols 13diols diols 1,3 3 multistep multi step synthesis 2,2dimethoxypropane 22dimethoxypropane dimethoxypropane 2,2 2,2DMP 22DMP DMP (2,2-DMP 2,2dimethyl4hydroxymethyl1,3dioxolane 22dimethyl4hydroxymethyl13dioxolane dimethylhydroxymethyldioxolane dimethyl 4 hydroxymethyl dioxolane solketal (solketal USY HBeta HZSM5 HZSM 5 HZSM- temperature °C C 2,2DMP/glycerol 22DMPglycerol DMPglycerol DMP/glycerol ratio time h 0.055 0055 0.05 0 05 0.05- wt.%. wt wt.% . wt.%) 2, 9697% 9697 96 97 96-97 tested value strongweak strong weak observed topology surface production 3diols 13 2dimethoxypropane 22 2DMP 2dimethyl4hydroxymethyl1 3dioxolane 055 005 0.0 wt. 969 9 96-9 dimethylhydroxymethyl 2dimethyl4hydroxymethyl 00 0. 96-

Methods used to determine pesticide residues in teas typically require several laborious steps for sample preparation before analysis. In this study, a simple method based on solid-liquid extraction with low-temperature partitioning (SLE/LTP) and analysis by gas chromatography coupled to mass spectrometry (GC-MS) was optimized and validated for the determination of pesticide residues (pirimiphos-methyl, flutriafol, cyproconazole, and bifenthrin) in dehydrated green tea leaves. After low-temperature partitioning (LTP), the extract in acetonitrile was subjected to a clean-up step using primary secondary amine (PSA) and octadecylsilane (C18) as sorbents for chlorophyll removal. The optimized SLE/LTP-GC-MS method was validated, and it proved to be effective and selective for extracting pesticides from green tea samples, presenting limit of detection (LOD) and limit of quantification (LOQ) of 0.015 and 0.050 mg kg-1, respectively. Recovery ranged between 81-111%, coefficients of variation were less than 16% and coefficients of determination (R2) were greater than 0.990. The optimized and validated method was applied to green tea and 13 other tea varieties sampled in South and Southeast regions of Brazil. The pesticides under study were detected in some of those samples at values higher than the maximum residue limits (MRLs) allowed by the European Union. solidliquid solid liquid lowtemperature low temperature SLE/LTP SLELTP SLE LTP (SLE/LTP GCMS GC MS (GC-MS pirimiphosmethyl, pirimiphosmethyl pirimiphos methyl, methyl (pirimiphos-methyl flutriafol cyproconazole bifenthrin leaves LTP, , (LTP) cleanup clean up PSA (PSA C18 C (C18 removal SLE/LTPGCMS SLELTPGCMS LOD (LOD LOQ (LOQ 0015 0 015 0.01 0050 050 0.05 kg1, kg1 kg 1, 1 kg-1 respectively 81111%, 81111 81 111%, 111 81-111% 16 R2 R (R2 0990 990 0.990 Brazil MRLs (MRLs Union (LTP C1 (C1 LTPGCMS 001 01 0.0 005 05 kg- 81111% 8111 8 111% 11 81-111 (R 099 99 0.99 (C 00 0. 811 81-11 09 9 0.9 81-1 81-

The aim of this study was to effectively remove Fe2+ by using ozone microbubbles in bottled mineral water to prevent sediment from occurring during storage and increase shelf life. By considering the characteristics of mineral water with low solubility of ozone and high CO2 content, a suitable ozone injection step was chosen and a new mineral water treatment method using microbubbles was proposed. As a result of the treatment of the bottled mineral water with ozone microbubbles, the concentration of the iron ion was reduced from 0.14 to 0.01 mg L-1, and the shelf life increased to 360 days. During the treatment, the concentrations of K+ and Na+ were almost unchanged, and the deposition time was reduced to one-third compared to the natural oxidation. Fe2 Fe CO content proposed 014 0 14 0.1 001 01 0.0 L1, L1 L 1, 1 L-1 36 days K Na unchanged onethird one third oxidation 0. 00 L- 3

Pinacolone and benzopinacolone derivatives are an important class of organic compounds due to their uses in polymer synthesis and more recently as biologically active compounds. The conventional synthesis of such molecules is generally done in two steps, the pinacol coupling followed by the pinacol/pinacolone rearrangement. Aiming to prepare benzopinacolone derivatives in a greener fashion, we present here a one-step synthesis from acetophenones in the presence of zinc and tert-butyl chloride. To develop the methodology, conditions to prepare 3,3-diphenyl-2-butanone from acetophenone were optimized. To assess the scope, different substituted acetophenones were tested, resulting in the corresponding benzopinacolones at moderate yields (20-50%) with high purity. Structural elucidations were performed by 1H and 13C NMR (nuclear magnetic resonance spectroscopy) and GC-MS (gas chromatography-mass spectrometry). steps pinacolpinacolone pinacolone rearrangement fashion onestep one step tertbutyl tert butyl chloride methodology 3,3diphenyl2butanone 33diphenyl2butanone diphenylbutanone 3,3 diphenyl 2 butanone 3 optimized scope tested 2050% 2050 20 50% 50 (20-50% purity H C nuclear spectroscopy GCMS GC MS gas chromatographymass chromatography mass spectrometry. spectrometry . spectrometry) 3diphenyl2butanone 33 3, 205 5 (20-50 (20-5 (20- (20 (2 (

Efavirenz, an antiretroviral drug, is a class 2 according to the biopharmaceutics classification system. Many dissolution enhancement systems have been tried and our group had success using wet milling to decrease particle size and granules were obtained by spray and freeze drying. In this paper we present data related to the upgrade in the process, raising the solids concentration in the suspension from 10 to 50% (m/v) and changing the drying step for a fluid bed granulation. After that, tablets were obtained. Granules and tablets were fully evaluated and a pharmacokinetic study was also performed with the granules. By powder X-ray diffraction (PXRD) and Fourier transform infrared spectroscopy (FTIR) it was possible to prove that there was no phase transition in the sample after milling and drying. Dissolution efficiency of 4 from 5 granules was higher than 90%, considering 83% for the raw material. Tablets were technically approved but the dissolution was impacted and just 2 out of 11 showed results > 80%. A high enhancement in the bioavailability was also observed, around 172%. So, it is possible to conclude that efavirenz microcrystals with enhanced dissolution and bioavailability can be formulated into tablets and are a viable system to develop a new drug product. Efavirenz process 1 50 m/v mv m v (m/v granulation Xray X ray PXRD (PXRD FTIR (FTIR 90 90% 83 material 80 80% observed 172 172% So product 9 8 17

Sensor calibration for dissolved gas is a critical and necessary step to ensure consistent sensor response over time and under different conditions. In this work, we describe a 3D printed device that facilitates the calibration of gas sensors. The device effectively maintained gas concentration in the solution for at least 2 h. Analytical curves were constructed in the pH range of 2 to 12, yielding determination coefficients surpassing 0.98. The chosen method proved suitable for the calibration of the sensors. Furthermore, using accurate and reliable reference sensors contributed to obtaining precise and trustworthy measurements. conditions work D h 12 098 0 98 0.98 Furthermore measurements 1 09 9 0.9 0.

The training of Chemistry teachers in Brazil has been researched for a few decades and studies point out to training gaps that reverberate in teaching, especially in basic education. One of the contents covered in this step and which is part of the high school curriculum is radioactivity, which is related, among other things, to radiological and radioactive accidents that occur around the world. Thus, this research sought to investigate how the training provided to Chemistry undergraduates in the state of Goiás is in relation to the subject of radioactivity, since in 1987 a radiological accident occurred in Goiânia with a Cesium 137 source. This is a qualitative research, data were collected via questionnaire and the analytical technique used was discursive textual analysis (ATD). The results showed that due importance was not given to the radiological accident, since there is evidence of training gaps in relation to the topic of radioactivity in Chemistry degree courses in the state of Goiás, where the accident occurred, and the contents on radioactivity are either not addressed, or the approach is superficial. This fact directly impacts the teacher’s performance in basic education, when working on this content with their students. teaching education related things world Thus 198 13 source ATD. ATD . (ATD) addressed superficial teacher s students 19 1 (ATD

ABSTRACT Introduction: toxoplasmosis is a parasitosis that causes serious infections in immunocompromised patients and newborns. The serological status of the mother influences the possibility of transmission to the fetus if the infection is contracted during pregnancy. It is necessary to know the prevalence of this condition in pregnant women as a first step to carry out actions aimed at knowing the real burden of the disease and reducing this pathology. Objective: determine the IgG and IgM seroprevalence of toxoplasmosis in pregnant women and newborns from January 2017 to January 2021. Methodology: pregnant women in their immediate peripartum period and newborns up to one week of extrauterine life were included. IgG and IgM were measured in peripheral blood by the ECLIA method. The calculated sample size was 800 per group, for the study period. Results: 2524 newborns and 2029 pregnant women were included. The prevalence of IgG was 97.5 % and 97 % for pregnant women and newborns, respectively, and IgM: 4.7 % and 1.9% for women and newborns, respectively. The seroprevalence trends remained constant for IgG for both groups, while IgM showed a slight increasing trend. Conclusions: IgG seroprevalence was close to 100% in both populations, probably due to the transplacental transfer of IgG, however, a small percentage of IgM was noted, a probable marker of acute infection.

RESUMEN Introducción: la toxoplasmosis es una parasitosis que causa infecciones graves en inmunocomprometidos y recién nacidos. El estado serológico de la madre influye en la posibilidad de transmisión al feto si la infección es contraída durante el embarazo. Es necesario conocer la prevalencia de esta afección en gestantes como primer paso para realizar acciones tendientes a conocer la carga real de la enfermedad y disminuir esta patología. Objetivo: determinar la seroprevalencia IgG e IgM de toxoplasmosis de gestantes y recién nacidos periodo de enero 2017 a enero 2021. Metodología: se incluyeron las gestantes en su periodo periparto inmediato y recién nacidos hasta la semana de vida extrauterina. Se midió IgG e IgM en sangre periférica por método ECLIA. El tamaño muestral calculado fue de 800 por grupo, para el periodo de estudio. Muestreo no probabilístico de casos consecutivos. Resultados: fueron incluidos 2524 recién nacidos y 2029 gestantes. Las prevalencias de IgG fueron de 97,5 % y 97 % para gestantes y recién nacidos respectivamente, y la IgM: 4,7% y 1,9 % para mujeres y recién nacidos respectivamente. Las tendencias de las seroprevalencias se mantuvieron constantes para IgG para ambos grupos, mientras que la IgM presentó una leve tendencia al aumento. Conclusiones: la seroprevalencia de IgG fue cercana al 100 % en ambas poblaciones, probablemente debido a la transferencia transplacentaria de IgG, sin embargo, se nota un porcentaje pequeño de IgM, probable marcador de infección aguda.

ABSTRACT Introduction: The introduction of Single-Port (SP) platform opened the field to new surgical options, allowing to perform major urological robot-assisted procedures extraperitoneally and with a supine patient positioning (1–3). Nevertheless, a comprehensive description of different supine access options is still lacking (4–6). In this light, we provided a step-by-step guide of SP extraperitoneal supine access options also exploring preliminary surgical outcomes. Materials and methods: Transvesical access was performed by a transversal incision 3cm above the pubic bone, after the anterior abdominal sheet incision, the bladder was insufflated with a flexible cystoscope and the detrusor muscle was incised at the level of the bladder dome. Similarly, the extraperitoneal access was carried out with a 4cm incision above the pubic bone, once visualized the preperitoneal space the prevesical fat was gently spread. The Low Anterior Access was performed with a 3cm incision at the McBurney point, the abdominal muscles were then spread. A gentle dissection was used laterally to develop the retroperitoneal space. Results: Overall, sixteen different procedures were performed with supine extraperitoneal access on 623 consecutive patients. No intraoperative conversions occurred. The median access time was 16 (IQR 12-21), 11 (IQR 7-14) and 14 (IQR 10-18) minutes in case of transvesical, extraperitoneal and low anterior access, respectively. Notably, 81.5 % of patients were discharged on the same day with a postoperative opioid free rate of 73%. Conclusion: The Atlas provides a comprehensive step-by-step guide to successfully perform all major urological SP procedures extraperitoneally and with supine patient positioning. Introduction SinglePort Single Port (SP robotassisted robot assisted 1–3. 13 1–3 . 1 3 (1–3) Nevertheless 4–6. 46 4–6 4 6 (4–6) light stepbystep step outcomes methods cm bone dome Similarly spread point Results Overall 62 occurred IQR 1221, 1221 12 21 , 12-21) 714 7 7-14 1018 10 18 10-18 transvesical respectively Notably 815 81 5 81. 73 73% Conclusion 1– (1–3 4– (4–6 122 2 12-21 71 7-1 101 10-1 8 (1– (4– 12-2 7- 10- (1 (4 12- (

Abstract Introduction. Tomato (Solanum lycopersicum L.) is considered one of the most important crops in the world. Genetic improvement of tomatoes has employed different methods to generate variability, and mutation the induction of mutations is one of the techniques used to obtaining improved varieties. The first step in using this tool is studying radiosensitivity to determine the irradiation dose to be used. Objective. To determine the radiosensitivity and the dose of cobalt-60 gamma rays to be used in three tomato varieties. Materials and methods. This research was conducted in January 2020 at the Instituto Nacional de Ciencias Agrícolas of Cuba. Seeds of three tomato varieties were irradiated with cobalt-60 gamma rays, with doses ranging from 100 Gy to 900 Gy, at intervals of 100 Gy; an unirradiated control was also used. The percentage of germination, plant height, and seedling survival were evaluated. Results. It was observed that higher irradiation dose reduced germination, survival, and plant height. The IDIAP T-7 and IDIAP T-9 varieties reduced germination at a dose of 400 Gy, while the DINA RPs cultivar reduced germination at 300 Gy. Genotypes showed survival values below 50 % at a dose of 800 Gy. Height reduction began at doses of 200 Gy for IDIAP T-7, 300 Gy for DINA RPs, and 400 Gy for IDIAP T-9. Conclusion. The DL-50 doses were identified as 692, 588, and 630 Gy, and the GR-50 doses were 407, 467, and 380 Gy for the IDIAP T-7, IDIAP T-9, and DINA RPs varieties, respectively. The GR-50 dose of each variety was selected for the mutation breeding program.

Resumen Introducción. El tomate (Solanum lycopersicum L.) es considerado uno de los cultivos más importantes en el mundo. La mejora genética del tomate ha empleado diferentes métodos para generar variabilidad, la inducción de mutaciones es una de las técnicas empleadas en la obtención de variedades mejoradas. El primer paso, en el uso de esta herramienta, es el estudio de radiosensibilidad para determinar la dosis de irradiación a emplear. Objetivo. Determinar la radiosensibilidad y la dosis de rayos gamma de cobalto-60 a emplear en tres variedades de tomate. Materiales y métodos. Esta investigación se desarrolló en enero del año 2020, en el Instituto Nacional de Ciencias Agrícolas de Cuba. Se irradiaron con rayos gamma de Cobalto-60 semillas de tres variedades de tomate, con dosis entre 100 Gy y 900 Gy, con intervalos de dosis de 100 Gy; también se empleó un control sin irradiar. Se evaluaron el porcentaje de germinación, la altura y la sobrevivencia de las plántulas. Resultados. Se observó que a mayores dosis de irradiación se redujo la germinación, la sobrevivencia y la altura de las plántulas. Las variedades IDIAP T-7 e IDIAP T-9 redujeron su germinación a la dosis de 400 Gy y el cultivar DINA RPs a los 300 Gy. Los genotipos presentaron valores de sobrevivencia por debajo del 50 % a la dosis de 800 Gy. La reducción de altura inició a la dosis de 200 Gy para la variedad IDIAP T-7, 300 Gy para DINA RPs y 400 Gy para IDIAP T-9. Conclusión. Las dosis de DL-50 identificadas fueron 692, 588 y 630 Gy y las dosis deGR-50 fueron 407, 467 y 380 Gy para las variedades IDIAP T-7, IDIAP T-9 Y DINA RPs, respectivamente. Se seleccionó la dosis GR-50 de cada una de las variedades para el programa de mejora por mutaciones.

Abstract Since 2010, there has been an approximate annual increase of 1% in CO2 emissions due to buildings. The reduction of energy consumption and consequently the mitigation of Greenhouse Gases are global goals due to the global issue of climate change. A key step in achieving these goals is to improve the energy performance of buildings, thereby reducing energy consumption and emissions generated by the built environment. In this regard, the present research focuses on identifying and estimating strategies to reduce energy consumption in buildings known as Community Police Units (UPCs) located in the city of Guayaquil, which corresponds to a very hot and humid climate zone. The study consists of statistical analysis of the historical energy performance of 43 buildings sharing the same architectural design. Additionally, the selection of a representative building was made for energy use monitoring and energy simulation. The results revealed a potential saving of 16 000 kWh per year by reducing the control temperature of the air conditioning system by 1°C and adjusting the on/off schedule. This is equivalent to 1 150 USD and 5 metric tons of CO2 emissions.

Resumen: Desde el 2010 existe un incremento aproximado del 1% anual respecto a las emisiones de CO2 a causa de los edificios. La disminución del consumo energético y consecuentemente la mitigación de los Gases de Efecto Invernadero son metas a nivel mundial debido a la problemática global del cambio climático. Un paso clave para alcanzar estas metas es mejorar el desempeño energético de las edificaciones y de esta manera reducir la energía consumida y las emisiones generadas por el parque edificado. En este sentido, la presente investigación se centra en identificación y estimación de estrategias para reducir el consumo de energía de los edificios llamados Unidades de Policía Comunitaria (UPC) localizadas en la ciudad de Guayaquil, y que corresponde a una zona climática húmeda muy calurosa. El estudio está compuesto por el análisis estadístico del rendimiento energético histórico de 43 edificios, que comparten el mismo diseño arquitectónico. Además, se realizó la selección de un edificio representativo para el monitoreo del uso de energía y simulación energética. Los resultados evidenciaron un potencial de ahorro de 16 000 kWh al año, al disminuir 1°C la temperatura de control del sistema de climatización y modificando el horario de encendido y apagado. Esto es equivalente a 1 150 USD y 5 toneladas equivalentes de CO2.

Abstract The shear behavior of reinforced concrete beams, particularly those with steel fiber reinforced concrete (SFRC), remains a challenge for the scientific community, as it involves complex material behavior. However, the advancement of the digital image correlation (DIC) technique has facilitated the acquisition of essential experimental data for modeling the mechanical behavior of SFRC, contributing to a better understanding of the problem and the shear resistance mechanisms. In this study, eight reinforced concrete beams, with and without steel fibers, were evaluated using the DIC technique to quantify the resistance mechanisms, with a focus on models that may represent the contribution of the fibers. The results revealed that the predominant mechanisms in shear behavior were aggregate interlock and the steel fibers. This study also represents a step towards a more rational understanding of shear behavior, while identifying gaps that require further investigation, such as the influence of fibers on all resistance mechanisms and the formulation of the contribution of steel fibers. beams SFRC , (SFRC) community However (DIC investigation (SFRC

Resumo O entendimento do comportamento à força cortante de vigas de concreto armado, especialmente as vigas de concreto reforçado com fibras de aço (CRFA), ainda é um desafio para a comunidade científica, devido à complexidade do comportamento dos materiais. No entanto, a evolução da técnica de correlação por imagem digital (CID) permitiu a obtenção de dados experimentais fundamentais para a modelagem do comportamento mecânico do CRFA, contribuindo para um melhor entendimento do problema e dos mecanismos resistentes à força cortante. Neste sentido, o presente estudo avalia oito vigas de concreto armado, com e sem fibras de aço, utilizando a técnica CID para quantificar os mecanismos resistentes, com foco nos modelos que podem representar a contribuição das fibras. Os resultados revelaram que os mecanismos preponderantes no comportamento à força cortante foram o engrenamento dos agregados e as fibras de aço. Este estudo representa um avanço no entendimento mais racional do comportamento à força cortante, assim como identifica lacunas que requerem estudos mais aprofundados, como a influência das fibras em todos os mecanismos resistentes e a formulação da contribuição das fibras de aço. armado CRFA , (CRFA) científica materiais entanto (CID sentido aprofundados (CRFA

SUMMARY: The aim of this study was to determine the effects 12 weeks Step aerobics program on anthropometric characteristics and body composition in young females. A total of 94 female participants were randomly divided in two groups. The E group subsample consisted of 44 participants (20±0.4 years), who had three Step aerobics program trainings per week and the C group subsample consisted of 50 participants (20±0.6 years), who did not take part in any organized exercise program and only conducted their regular daily activities. The following measurements were used: Body height, body weight, waist size, abdomen skinfold, back skinfold, upper arm skinfold, upper leg skinfold, body fat percentage and muscle mass percentage. Upon the Step aerobics program implementation, there was a body fat reduction by 4.9 % and waist size by 7.7 %, while the muscle mass was increased by 9.4 %. Above mentioned skinfolds showed a reduction (p>0.05) at the final measurement compared to the initial one - abdomen by 25 %, upper leg by 23.7 %, back by 15.7 % and upper arm by 3.5 %. Likewise, there was a body fat percentage reduction by 2.1 % and BMI by 6.4 %. Based on the obtained results, we can conclude that the Step aerobics training is efficient in reducing subcutaneous fat tissue and viscelar fat, as well as in increasing muscle mass.

RESUMEN: El objetivo de este estudio fue determinar los efectos de un programa aeróbico Step de 12 semanas sobre las características antropométricas y la composición corporal en mujeres jóvenes. Un total de 94 mujeres participantes fueron divididas aleatoriamente en dos grupos. La submuestra del grupo E estuvo compuesta por 44 participantes (20 ± 0,4 años), que realizaron tres entrenamientos del programa aeróbico Step por semana y la submuestra del grupo C estuvo compuesta por 50 participantes (20 ± 0,6 años), que no participaron en ningún programa de ejercicio organizado y sólo realizaron sus actividades diarias habituales. Se utilizaron las siguientes mediciones: altura corporal, peso corporal, tamaño de cintura, pliegue cutáneo del abdomen, pliegue cutáneo de la espalda, pliegue cutáneo de la parte superior del brazo, pliegue cutáneo de la parte superior de la pierna, porcentaje de grasa corporal y porcentaje de masa muscular. Al implementar el programa aeróbico Step, hubo una reducción de la grasa corporal de un 4,9 % y del tamaño de la cintura de un 7,7 %, mientras que la masa muscular aumentó un 9,4 %. Los pliegues cutáneos mencionados anteriormente mostraron una reducción (p>0,05) en la medición final en comparación con la inicial: abdomen en un 25 %, parte superior de la pierna en un 23,7 %, espalda en un 15,7 % y parte superior del brazo en un 3,5 %. Asimismo, hubo una reducción del porcentaje de grasa corporal del 2,1 % y del IMC del 6,4 %. Con base en los resultados obtenidos, podemos concluir que el entrenamiento aeróbico Step es eficaz para reducir el tejido adiposo subcutáneo y la grasa visceral, así como para aumentar la masa muscular.

SUMMARY OBJECTIVE: Vascular endothelial growth factor is a signaling protein created by cells performing important bodily functions. Vascular endothelial growth factor is abundant in the lung, and plasma levels are elevated in patients with severe pulmonary arterial hypertension. An association between soluble urokinase plasminogen activator receptor, an inflammatory biomarker, and soluble urokinase plasminogen activator receptor levels and interstitial pulmonary and vascular involvement (e.g., development of pulmonary hypertension) has been shown in SSc patients. The tricuspid annular plane systolic excursion/systolic pulmonary artery pressure ratio, which has been recommended as a useful diagnostic tool in the last guideline, is one of the additional echocardiographic signs suggestive of pulmonary hypertension. We aimed to examine whether these biomarkers contribute to the diagnosis and management of pulmonary hypertension. METHODS: Patients with group E chronic obstructive lung disease were included in this prospective study. Demographic data, echocardiographic signs about the right ventricle (right atrium area, tricuspid annular plane systolic excursion/systolic pulmonary artery pressure, fractional area change, and right ventricular outflow tract), and peripheral blood analysis were examined and recorded. RESULTS: A total of 70 patients, 12 of whom were female, were analyzed in the study. The mean age was 66.6±8.7 years. The mean vascular endothelial growth factor-A and soluble urokinase plasminogen activator receptor were 91.05±70.7 and 955.8±571.1, and their Pearson correlation coefficients between vascular endothelial growth factor-A and tricuspid annular plane systolic excursion/systolic pulmonary artery pressure, and soluble urokinase plasminogen activator receptor and tricuspid annular plane systolic excursion/systolic pulmonary artery pressure ratio were 0.341 (p=0.004) and −0.045 (p=0.70), respectively. The linear regression model included four variables with significant correlation (vascular endothelial growth factor-A, right atrium area, fractional area change, and right ventricular outflow tract). Three steps were performed, and adjusted r2 was 0.22, 0.22, 0.20, and p<0.001 for each step. Vascular endothelial growth factor-A and right ventricular outflow tract remained in the last step. It was detected a standardized coefficient beta of 0.322 (p=0.004) and a 95%CI 0.000–0.001 for vascular endothelial growth factor-A. CONCLUSION: Vascular endothelial growth factor-A is correlated with the tricuspid annular plane systolic excursion/systolic pulmonary artery pressure ratio and not with soluble urokinase plasminogen activator receptor. OBJECTIVE functions hypertension biomarker e.g., eg e g (e.g. excursionsystolic excursion guideline METHODS study data change tract, , tract) recorded RESULTS 7 1 female 66687 66 6 8 66.6±8. years factorA 9105707 91 05 91.05±70. 95585711 955 571 955.8±571.1 0341 0 341 0.34 p=0.004 p0004 p 004 (p=0.004 0045 045 −0.04 p=0.70, p070 p=0.70 (p=0.70) respectively factorA, A, tract. . performed r 022 22 0.22 020 20 0.20 p0001 001 p<0.00 step 0322 322 0.32 95CI CI 95 00000001 000 0.000–0.00 factorA. A. CONCLUSION e.g. (e.g 6668 66.6±8 910570 9 91.05±70 9558571 57 955.8±571. 034 34 0.3 p=0.00 p000 00 (p=0.00 04 −0.0 p07 p=0.7 (p=0.70 02 2 0.2 p<0.0 032 32 0000000 0.000–0.0 e.g 666 66.6± 91057 91.05±7 955857 5 955.8±571 03 3 0. p=0.0 p00 (p=0.0 −0. p0 p=0. (p=0.7 p<0. 000000 0.000–0. 66.6 9105 91.05± 95585 955.8±57 (p=0. −0 p=0 p<0 00000 0.000–0 66. 910 91.05 9558 955.8±5 (p=0 − p= p< 0000 0.000– 91.0 955.8± (p= 0.000 91. 955.8 (p 0.00 955. 0.0