This study aims to propose a new method for derivatizing fatty acids (FAs) from human milk (HM), eliminating the lipid extraction step, and simplifying the preparation for gas chromatography with a flame ionization detection (GC-FID) analysis to quantify the FAs. The Design Expert software optimized the reaction times, concentrations, and sample amount. The proposed method (PM) was validated for lyophilized HM and results for the figures of merit for precision in relative standard deviation (RSD) (RSDintra-day 1.34-4.03% and RSDinter day 2.08 5.16%), accuracy (99.87-102.16%), and robustness are within a linear range of 3 to 38% lipids in HM samples. The atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) technique confirmed the efficiency of PM by expressing the molecular composition of triacylglycerol formed by FAs from the GC-FID technique. The PM requires a small sample size and conducts derivatization directly in the sample matrix, minimizing extraction errors. (FAs HM, , (HM) step GCFID GC FID (GC-FID times concentrations amount (PM RSD (RSD RSDintraday RSDintra 1.344.03% 134403 1.34 4.03% 1 34 4 03 1.34-4.03 208 2 08 2.0 5.16%, 516 5.16% 5 16 5.16%) 99.87102.16%, 998710216 99.87 102.16% 99 87 102 (99.87-102.16%) 38 samples ASAPMS/MS ASAPMSMS ASAP MS/MS MS (ASAP-MS/MS matrix errors (HM 344 1.344.03 13440 134 1.3 403 4.03 0 1.34-4.0 20 2. 51 5.16 87102 99.87102.16% 99871021 9987 99.8 10216 102.16 9 8 10 (99.87-102.16% ASAPMS MSMS 1.344.0 1344 13 1. 40 4.0 1.34-4. 5.1 8710 99.87102.16 9987102 998 99. 1021 102.1 (99.87-102.16 1.344. 4. 1.34-4 5. 871 99.87102.1 998710 102. (99.87-102.1 1.344 1.34- 99.87102. 99871 (99.87-102. 99.87102 (99.87-102 99.8710 (99.87-10 99.871 (99.87-1 (99.87- (99.87 (99.8 (99. (99 (9 (

ABSTRACT Due to the importance of grain production, and the need to work the soil under limited moisture, the aim of this study was to develop computer software to predict the tractive capacity of wheeled tractors for two types of tyre, the effect of different inflation pressures and levels of soil moisture. A mathematical model was developed to predict the tractive capacity of a 4 x 2 AFT tractor. Software was developed and tested by comparing the computational data with those from tests of a working tractor in the field. The parameters under evaluation were drawbar force and front and rear drive wheel slip. At both moisture levels, the bias tyres had the lowest front to rear slip ratio at the lower pressure; the ratios were similar when equipped with radial tyres. The bias tyres show maximum force at a slip ratio of 1.09 at the higher pressure and 1.03 at the lower, while for the radial tyres the ratio was 1.05 at the higher pressure and 1.07 at the lower. Software was developed to process data from the model, with the results used for estimating the tractive force of the tractor. The tractive force was overestimated by the model, which needs further adjustment for direct application in the field. production tyre field 109 1 09 1.0 103 03 105 05 107 07 10 0 1.

ABSTRACT The aim of this study was to verify the response of the melon accessions MR1 and C14 to gamma radiation in terms of germination and morphology.The melon accessions came from the germplasm collection of the Federal Rural University of the Semi-arid Region, and were submitted to five doses of gamma radiation (0, 100, 200, 300, 400 Gy) in four replications, each comprising 25 seeds, at the Center for Nuclear Energy in Agriculture of the University of São Paulo. The seeds were grown in a greenhouse at UFERSA employing a completely randomised design. The germination potential, rate and index were evaluated during the first stage of the trial. During the second stage, the germinated plants were transplanted into eight-litre buckets containing substrate and sand, in four replications of 15 plants each. The inflorescence, leaf blade and fruit were evaluated. The IMAGE J image analysis software was used to obtain the leaf and fruit data. The germination potential varied for the gamma-ray doses being tested, while the germination rate and index were not affected; phenotypic variations were seen in the leaves. The gamma-ray doses had no effect on the germination process. MR C C1 morphologyThe morphology Semiarid Semi arid Region 0, 0 (0 100 200 300 40 Gy 2 Paulo design trial eightlitre eight litre sand 1 inflorescence data gammaray ray tested affected leaves process ( 10 20 30 4 3

Worldwide, millions of people suffer from neurological and psychiatric conditions, such as schizophrenia, depression, and neurodegenerative diseases. These conditions are often linked to hyperactivity of monoamine oxidase B (MAO-B) in the brain, and inhibiting this enzyme can reduce oxidative stress, stabilize neurotransmitter levels, and alleviate symptoms. In this context, a pharmacophoric model was created using PharmaGist with 31 hits extracted from BindingDB to enable a pharmacophoric-based search of potential MAO-B inhibitors in ZincPharmer’s chemical space, followed by the building of 10 conformers for each molecule in the software OMEGA, screening for structural and electrostatic similarity to safinamide using ROCS (Rapid overlay of chemical structures) and EON (Electrostatic similarity for lead-hopping), respectively. In addition, the molecular docking with Gold using the score function was performed to rank the ligands with the highest probabilities of affinity to MAO-B and then discriminate those with suitable physical chemical, pharmacokinetic, and toxicological features using the tools QikProp, SwissADME, and DEREK. Four potential ligands with selective MAO-B inhibitor properties were identified, which show promise as future therapeutic agents for MAO-B-related disorders. Worldwide schizophrenia depression diseases MAOB MAO (MAO-B brain stress levels symptoms context 3 pharmacophoricbased based ZincPharmers ZincPharmer s space 1 OMEGA Rapid structures Electrostatic leadhopping, leadhopping lead hopping , lead-hopping) respectively addition pharmacokinetic QikProp SwissADME DEREK identified MAOBrelated related disorders lead-hopping

Gas chromatography coupled to mass spectrometry (GC-MS) has been widely used, and along with other methodologies such as derivatization, is a powerful tool to analyze phytocompounds, including cuticular wax components. The loss of structural information upon fragmentation of waxes makes it difficult to interpret and identify the GC-MS mass spectra of these compounds with the traditional methods, as digital libraries. Therefore, as a new complementary alternative to traditional methods of identifying aliphatic acyclic lipids based on mass spectra, we present the new software WaxAlly in this technical note. WaxAlly is a new software created based on simple algorithms, enabling the rapid recognition by the user of eight aliphatic acyclic lipid classes, including alkanes, alkenes, aldehydes, ketones, esters, and TMS derivatives of free fatty acids, primary and secondary alcohols, and their homologues with carbon chains varying between 10 and 100 carbons. Additionally, the WaxAlly software provides a section for data organization, internet comparison to NIST and PubChem databases, and academic information about mass fragmentation of acyclic lipids. The software has proven to be a very useful complementary technique in identifying plant wax lipid homologues, and new fragmentation patterns of lipid classes can be added in the future to improve the program. GCMS GC MS (GC-MS used derivatization phytocompounds components libraries Therefore note algorithms alkanes alkenes aldehydes ketones esters acids alcohols 1 carbons Additionally organization databases program

Alzheimer’s disease (AD) is a most common form of dementia and results in memory loss, disorientation, impaired thinking and changes in personality as well as mood. The dementia cases mostly turn into the Alzheimer’s disease (AD). Another disease, the Parkinson’s disease (PD), mainly affects dopaminergic neurons in substantia nigra. A series of previously synthesized quinolone-triazole hybrids were investigated and evaluated for their anti-acetyl cholinesterase and against monoamine oxidase reactive properties through in silico characterization. The ground state electronic properties were determined by electron density of the compounds through DFTs (density functional theory) calculation. The geometries of all compounds were optimized using Becke-3-parameter-Lee-Yang-Parr (B3LYP) method and 6-311g basis set. Docking studies were conducted using the AutoDock and Molecular Operating Environment (MOE) software and the results of the potent derivatives found to be encouraging. The data confirmed that all the compounds have drug-like properties and was further confirmed by ADMET (absorption, distribution, metabolism, excretion and toxicity) properties. Alzheimers Alzheimer s AD (AD loss disorientation mood AD. . Parkinsons Parkinson PD, PD , (PD) nigra quinolonetriazole quinolone triazole antiacetyl anti acetyl characterization theory calculation Becke3parameterLeeYangParr BeckeparameterLeeYangParr Becke 3 parameter Lee Yang Parr B3LYP BLYP B LYP (B3LYP 6311g g 6 311g set MOE (MOE encouraging druglike drug like absorption, absorption (absorption distribution metabolism toxicity (PD parameterLeeYangParr

The inhibition of the fatty acid amide hydrolase (FAAH), an endocannabinoid system component, emerged as a potentially new therapeutic target for a range of clinical disorders such as acute and chronic pain. Some α-ketoheterocycle derivatives demonstrated interesting analgesic and anti-inflammatory activities in vitro. Ligand-Based Drug Design techniques such as knowledge graph convolutional networks (kGCN) and hologram quantitative structure-activity relationship (HQSAR) using α-ketoheterocycle derivatives from five different datasets were generated to discover the relation between the chemical structures and the inhibition activity. Meanwhile, structure-based drug design simulations as interaction fields (MIF), molecular docking, and ligand sites studies (LSI) from FAAH were performed using Autogrid software and FTmap/FTsite servers. The results of both studies were merged to propose predictive models. The resulting kGCN model demonstrated adequate accuracy area under the curve by receiver operating characteristic (AUC-ROC 0.7922). From contribution maps of the Ligand-Based Drug Design (LBDD) models and the generated probes using MIF and LSI, it was observed that the oxazole ring, the ketone group, and the apolar chain present in the structures of the inhibitors are important, besides the evidence of the Cys269 and Val270 residues importance for the potential interaction, confirmed by carried docking studies. These fragments and structural information can be used to carry out new FAAH potential inhibitors studies and report kGCN as an accurate classification technique. FAAH, , (FAAH) component pain αketoheterocycle α ketoheterocycle antiinflammatory anti inflammatory vitro LigandBased Ligand Based (kGCN structureactivity structure activity HQSAR (HQSAR Meanwhile structurebased based MIF, (MIF) LSI (LSI FTmapFTsite FTmap FTsite servers AUCROC AUC ROC 0.7922. 07922 0.7922 . 0 7922 0.7922) LBDD (LBDD ring group important Cys Cys26 Val Val27 technique (FAAH (MIF 0792 0.792 792 Cys2 Val2 079 0.79 79 07 0.7 7 0.

Chemometrics is gaining more and more space in universities and industry. To contribute to the development and expansion of principal component analysis (PCA) teaching proposals, this work evaluated the answers of the students to a questionnaire and presented a didactic-pedagogical proposal that can contribute to solving the principal doubts achieved by students in the PCA interpretation. Based on data collected in a semi-open questionnaire, the proposal was developed based on methodological principles of historical-critical pedagogy, containing the objectives, the problems surrounding the topic, and the cultural instruments necessary for understanding and interpreting PCA in the discipline of chemometrics by using new resources and instruments, especially free software, Chemostat. This proposal covers topics related to the concept of chemometrics and PCA, the explanation of the data set, pre-processing, use of Chemostat software, and analysis of principal components. It is hoped that this proposal can promote a better understanding concerning PCA on the part of students, as well as effective incorporation of the phenomena that involve this tool and its applications, and interpretation. Likewise, it is expected that this didactic-pedagogical proposal can support new teaching practices in chemometrics subjects in higher education. industry (PCA proposals didacticpedagogical didactic pedagogical interpretation semiopen semi open historicalcritical historical critical pedagogy objectives topic software set preprocessing, preprocessing pre processing, processing pre-processing components applications Likewise education

RESUMO As atividades de recuperação insustentáveis (URAs) têm sido cada vez mais realizadas para responder à crescente procura global de alimentos, levando a graves repercussões nos ambientes terrestres. Um total de 120 amostras de solo representando 12 locais foram selecionadas aleatoriamente nos três tipos distintos de terra (PMFs, PALs e BHLs) a uma profundidade de 60 cm para avaliar os impactos das URAs na província de Nghe An Mountain, no Vietnã. A análise estatística dos parâmetros do solo foi realizada no software SPSS (versão 26.0) por meio de análise de variância unidirecional (ANOVA). As descobertas indicam diferenças distintas no tamanho das partículas do solo (SPS) entre os diferentes tipos de terreno (DLTs). Florestas virgens e mistas (PMFs) exibiram as menores proporções de areia, variando de 31,4 a 35,2%, enquanto colinas e terras nuas (BHLs) registraram as maiores proporções de areia, variando de 49,7 a 55,1%. Elevado densidade global (BD) foi observado em BHLs (1,36 ± 0,07 kg dm-3) e PMFs (0,89 ± 0,02 a 1,13 ± 0,03 kg dm-3) enquanto terras perenes e anuais (PALs) variaram de 1,17 ± 0,04 a 1,25 ± 0,08 kg dm-3. Os PMFs apresentaram baixos valores de porosidade total do solo (TSP) e teor de água do solo (SWC) com respectivas faixas de 32,97-36,18% e 4,72-6,15% enquanto PALs e BHLs exibiram valores elevados para TSP (39,25-43,19%; 43,97-49,62%). e SWC (7,39-10,07%; 9,98-12,74%). A capacidade de troca catiônica (CEC), Ca2+, K+ e Mg2+ registrou altos valores nos PALs em comparação aos PMFs e PALs, enquanto o conteúdo orgânico total (TOCs) detectou pouca variação entre os DLTs. No geral, as URAs aumentaram os efeitos adversos nas propriedades e funções do solo (SPFs) em toda a área de estudo. (URAs alimentos terrestres 1 PMFs, (PMFs 6 Mountain Vietnã versão 26.0 260 26 0 ANOVA. ANOVA . (ANOVA) SPS (SPS DLTs (DLTs) areia 314 31 4 31, 352 35 2 35,2% (BHLs 497 49 7 49, 551 55 55,1% BD (BD 1,36 136 36 (1,3 007 07 0,0 dm3 dm 3 dm-3 0,89 089 89 (0,8 002 02 113 13 1,1 003 03 (PALs 117 17 004 04 125 25 1,2 008 08 dm3. 3. (TSP (SWC 32,9736,18% 32973618 32,97 36,18% 32 97 18 32,97-36,18 4,726,15% 472615 4,72 6,15% 72 15 4,72-6,15 39,2543,19% 39254319 39,25 43,19% 39 43 19 (39,25-43,19% 43,9749,62%. 43974962 43,97 49,62% 62 43,97-49,62%) 7,3910,07% 7391007 7,39 10,07% 10 (7,39-10,07% 9,9812,74%. 9981274 9,98 12,74% 9 98 74 9,98-12,74%) CEC, CEC , (CEC) Ca2 Ca Ca2+ K Mg2 Mg TOCs (TOCs geral SPFs (SPFs estudo 26. (ANOVA (DLTs 35,2 5 55,1 1,3 (1, 00 0, dm- 0,8 8 (0, 11 1, 9736 32,9736,18 3297361 3297 32,9 3618 36,18 32,97-36,1 726 4,726,15 47261 472 4,7 615 6,15 4,72-6,1 2543 39,2543,19 3925431 3925 39,2 4319 43,19 (39,25-43,19 9749 43,9749,62% 4397496 4397 43,9 4962 49,62 43,97-49,62% 3910 7,3910,07 739100 739 7,3 1007 10,07 (7,39-10,07 9812 9,9812,74% 998127 998 9,9 1274 12,74 9,98-12,74% (CEC 35, 55, (1 (0 973 32,9736,1 329736 329 32, 361 36,1 32,97-36, 4,726,1 4726 47 4, 61 6,1 4,72-6, 254 39,2543,1 392543 392 39, 431 43,1 (39,25-43,1 974 43,9749,62 439749 439 43, 496 49,6 43,97-49,62 391 7,3910,0 73910 73 7, 100 10,0 (7,39-10,0 981 9,9812,74 99812 99 9, 127 12,7 9,98-12,74 ( 32,9736, 32973 36, 32,97-36 4,726, 6, 4,72-6 39,2543, 39254 (39,25-43, 43,9749,6 43974 43,97-49,6 7,3910, 7391 10, (7,39-10, 9,9812,7 9981 12, 9,98-12,7 32,9736 32,97-3 4,726 4,72- 39,2543 (39,25-43 43,9749, 43,97-49, 7,3910 (7,39-10 9,9812, 9,98-12, 32,973 32,97- 39,254 (39,25-4 43,9749 43,97-49 7,391 (7,39-1 9,9812 9,98-12 (39,25- 43,974 43,97-4 (7,39- 9,981 9,98-1 (39,25 43,97- (7,39 9,98- (39,2 (7,3 (39, (7, (39 (7 (3

ABSTRACT Unsustainable reclamation activities (URAs) have been increasingly conducted to respond to the rising global food demand, leading to severe repercussions on land environments. A total of 120 soil samples representing 12 sites were randomly selected in the three distinct land types (PMFs, PALs, and BHLs) to a depth of 60 cm. The impacts of URAs on soil properties and functions (SPFs) across the Nghe An mountainous province, Vietnam were assessed using the SPSS software (version 26.0) through a one-way analysis of variance (ANOVA). The findings indicated the distinct differences in soil particle size (SPS) among different land types (DLTs). Pristine and mixed forests (PMFs) exhibited the lowest sand ratios, ranging from 31.4 to 35.2%, while bare hills and lands (BHLs) recorded the highest sand ratios, ranging from 49.7 to 55.1%. High bulk density (BD) was observed in BHLs (1.36 ± 0.07 kg dm-3) and PMFs (0.89 ± 0.02 to 1.13 ± 0.03 kg dm-3) while perennial and annual lands (PALs) varied from 1.17 ± 0.04 to 1.25 ± 0.08 kg dm-3. PMFs showed low values for total soil porosity (TSP), and soil water content (SWC) with respective ranges of 32.97-36.18% and 4.72-6.15% while PALs and BHLs exhibited high values for TSP (39.25-43.19%; 43.97-49.62%), and SWC (7.39-10.07%; 9.98-12.74%). Cation exchange capacity (CEC), Ca2+, K+, and Mg2+ were recorded higher in PALs compared to PMFs and PALs while total organic contents (TOCs) detected little variation among DLTs. Overall, the URAs enhanced the adverse effects on the SPFs across the study area. (URAs demand environments 1 PMFs, (PMFs 6 cm (SPFs province version 26.0 260 26 0 oneway one way ANOVA. ANOVA . (ANOVA) SPS (SPS DLTs (DLTs) ratios 314 31 4 31. 352 35 2 35.2% (BHLs 497 49 7 49. 551 55 55.1% BD (BD 1.36 136 36 (1.3 007 07 0.0 dm3 dm 3 dm-3 0.89 089 89 (0.8 002 02 113 13 1.1 003 03 (PALs 117 17 004 04 125 25 1.2 008 08 dm3. 3. TSP, , (TSP) (SWC 32.9736.18% 32973618 32.97 36.18% 32 97 18 32.97-36.18 4.726.15% 472615 4.72 6.15% 72 15 4.72-6.15 39.2543.19% 39254319 39.25 43.19% 39 43 19 (39.25-43.19% 43.9749.62%, 43974962 43.97 49.62% 62 43.97-49.62%) 7.3910.07% 7391007 7.39 10.07% 10 (7.39-10.07% 9.9812.74%. 9981274 9.98 12.74% 9 98 74 9.98-12.74%) CEC, CEC (CEC) Ca2 Ca Ca2+ K K+ Mg2 Mg TOCs (TOCs Overall area 26. (ANOVA (DLTs 35.2 5 55.1 1.3 (1. 00 0. dm- 0.8 8 (0. 11 1. (TSP 9736 32.9736.18 3297361 3297 32.9 3618 36.18 32.97-36.1 726 4.726.15 47261 472 4.7 615 6.15 4.72-6.1 2543 39.2543.19 3925431 3925 39.2 4319 43.19 (39.25-43.19 9749 43.9749.62% 4397496 4397 43.9 4962 49.62 43.97-49.62% 3910 7.3910.07 739100 739 7.3 1007 10.07 (7.39-10.07 9812 9.9812.74% 998127 998 9.9 1274 12.74 9.98-12.74% (CEC 35. 55. (1 (0 973 32.9736.1 329736 329 32. 361 36.1 32.97-36. 4.726.1 4726 47 4. 61 6.1 4.72-6. 254 39.2543.1 392543 392 39. 431 43.1 (39.25-43.1 974 43.9749.62 439749 439 43. 496 49.6 43.97-49.62 391 7.3910.0 73910 73 7. 100 10.0 (7.39-10.0 981 9.9812.74 99812 99 9. 127 12.7 9.98-12.74 ( 32.9736. 32973 36. 32.97-36 4.726. 6. 4.72-6 39.2543. 39254 (39.25-43. 43.9749.6 43974 43.97-49.6 7.3910. 7391 10. (7.39-10. 9.9812.7 9981 12. 9.98-12.7 32.9736 32.97-3 4.726 4.72- 39.2543 (39.25-43 43.9749. 43.97-49. 7.3910 (7.39-10 9.9812. 9.98-12. 32.973 32.97- 39.254 (39.25-4 43.9749 43.97-49 7.391 (7.39-1 9.9812 9.98-12 (39.25- 43.974 43.97-4 (7.39- 9.981 9.98-1 (39.25 43.97- (7.39 9.98- (39.2 (7.3 (39. (7. (39 (7 (3

RESUMO: A estrutura do sistema radicular do cafeeiro Conilon tem sido aprimorada ao longo dos anos por meio de propagação e estudos genéticos e agronômicos em resposta às demandas por melhorias no desenvolvimento vegetal, na produção e na sobrevivência da espécie. Entende-se que a pesquisa científica desempenha um papel importante na geração de tecnologias e também na segurança das aplicações. Porém, há necessidade de refinar o código gerado para descobrir descobertas e tendências sobre o assunto. Nesse sentido, o presente estudo tem como objetivo realizar uma revisão bibliométrica e análise na base de dados Web of Science (WOS) sobre as principais contribuições de estudos, pesquisadores, organizações e países relevantes nas pesquisas acadêmicas sobre o enraizamento de Coffea canephora. Dados da base de dados WOS publicados entre 1982 e 2021 e sistematizados no software VOSviewer, mostraram um conjunto de 92 artigos, em que a maioria teve origem no Brasil e na França, tendo como principais grupos a Empresa Brasileira de Pesquisa Agropecuária, a Universidade Federal de Viçosa e o CIRAD. O estudo está dividido em quatro áreas: diversidade genética, propagação assexuada, nematologia, tolerância ao estresse hídrico e micropropagação, porém, destaca-se que no contexto das mudanças climáticas e seu impacto na produção e longevidade da cafeicultura brasileira, podemos ver o surgimento de pesquisas focadas no sistema radicular, integrando-o em linhas que exploram e integram temas como risco climático, gestão hídrica, tolerância à seca e irrigação por gotejamento, incluindo reflexões sobre o desempenho da agronomia cafeeira. RESUMO vegetal espécie Entendese Entende se aplicações Porém assunto sentido (WOS pesquisadores canephora 198 202 VOSviewer 9 artigos França Agropecuária CIRAD áreas genética assexuada nematologia micropropagação porém destacase destaca brasileira integrandoo integrando climático hídrica gotejamento cafeeira 19 20 1 2

ABSTRACT: The structure of the root system of the conilon coffee tree has improved over the years through propagation, genetic, and agronomic studies in response to demands for improvements in plant development, production, and survival of the species. Scientific research plays an important role in generating technologies and the security of applications. However, there is a need to refine the content generated to analyze discoveries and trends on the subject. Therefore, the present study conduct a bibliometric review and analysis on the main contributions of relevant studies, researchers, organizations, and countries in academic research on the rooting of Coffea canephora in the Web of Science (WOS) database. Data from the WOS database published between 1982 and 2021 and systematized in the VOSviewer software showed a set of 92 articles, the majority of which originated in Brazil and France, with the main groups being the Brazilian Agricultural Research Corporation, the Federal University of Viçosa, and CIRAD. The study was divided into five areas: genetic diversity, asexual propagation, nematology, tolerance to water stress, and micropropagation. However, in the context of climate changes and its impact on the production and longevity of Brazilian coffee farming, research focused on the root system has increased significantly, integrating it into lines that explore and integrate topics such as climate risk, water management, drought tolerance, and drip irrigation, including reflections on the performance of coffee agronomy. ABSTRACT propagation development species applications However subject Therefore researchers organizations (WOS 198 202 9 articles France Corporation Viçosa CIRAD areas diversity nematology stress micropropagation farming significantly risk management irrigation agronomy 19 20 1 2

ABSTRACT The search for new technologies capable of providing benefits to healthy eating is a global goal. The consumption of foods rich in carotenoids can prevent serious health problems. Thus, the availability of foods high in carotenoids that are accessible to the majority of the population is essential. Lettuce (Lactuca sativa L.) is a suitable species for biofortification research based on genetic improvements. In this study, we aimed to select and obtain hybrid lettuce populations with high agronomic potential and biofortified carotenoid content. Ten lettuce lines registered in the “BG α Biofort” software were selected for hybridization. The crosses used to obtain the hybrids involved two strains rich in at least one of the pigments (chlorophyll and carotenoids), and 24 experimental hybrids were obtained. A total of 37 genotypes (strains, hybrids, and commercial cultivars) were evaluated using a randomized block design for agronomic variables and chlorophyll and carotenoid content. Hybrids 12 (UFU-189#3#4#1 × UFU MC BIOFORT 2) and 25 (UFU66#4#2 × UFU-189#3#4#1) had the potential to produce dual-purpose populations (yield and carotenoid biofortification). Hybrid 27 (UFU66#4#2 × UFU MC BIOFORT1) exhibited the best pigment balance. Thus, F2 hybrids 12, 25, and 27 should be obtained to produce new cultivars of biofortified red and green lettuce for curly, american, mini, and romaine lettuce segments. goal problems Thus essential Lactuca L. L improvements study content BG Biofort hybridization carotenoids, , carotenoids) 2 3 strains, (strains 1 UFU189#3#4#1 UFU189341 189#3#4#1 189 4 (UFU-189#3#4# UFU66#4#2 UFU6642 UFU66 (UFU66#4# UFU-189#3#4#1 dualpurpose dual purpose yield biofortification. . biofortification) BIOFORT1 balance F curly american mini segments UFU189 UFU189#3#4# UFU18934 189341 189#3#4# 18 (UFU-189#3#4 UFU66#4# UFU664 UFU6 (UFU66#4 UFU-189#3#4# UFU18 UFU189#3#4 UFU1893 18934 189#3#4 (UFU-189#3# UFU66#4 (UFU66# UFU-189#3#4 UFU1 UFU189#3# 1893 189#3# (UFU-189#3 UFU66# (UFU66 UFU-189#3# UFU189#3 189#3 (UFU-189# (UFU6 UFU-189#3 UFU189# 189# (UFU-189 (UFU UFU-189# (UFU-18 UFU-189 (UFU-1 UFU-18 (UFU- UFU-1 UFU-

RESUMO A busca por novas tecnologias capazes de proporcionar benefícios para uma alimentação saudável tem sido meta mundial. A presença de alimentos ricos em carotenoides pode ser fundamental para prevenir sérios problemas de saúde. Aliar alimentos com altos teores de carotenoides e acessíveis para a maior parte da população é fundamental. A alface (Lactuca sativa L.) se apresenta como uma espécie adequada para investir em pesquisas com biofortificação a partir de melhoramento genético. Assim, o objetivo com este estudo foi selecionar híbridos de alface visando à obtenção de populações com potencial agronômico e biofortificadas para carotenoides. Dez linhagens de alface cadastradas no Software “BG α Biofort” foram utilizadas nas hibridações. Os cruzamentos para obtenção dos híbridos foram planejados envolvendo duas linhagens ricas em pelo menos um dos pigmentos (clorofila e carotenoides) sendo obtidos 24 híbridos experimentais. Foram avaliados 37 genótipos (linhagens, híbridos e cultivares comercias) em delineamento em blocos casualizados quanto as variáveis agronômicas e teores de clorofila e carotenoides. Foi verificado que os híbridos 12 (UFU-189#3#4#1 × UFU MC BIOFORT 2) e 25 (UFU66#4#2 × UFU-189#3#4#1) possuem potencial para obtenção de populações de duplo propósito (produtividade e biofortificada para carotenoide). O híbrido 27 (UFU66#4#2 × UFU MC BIOFORT1) foi o que apresentou maior equilíbrio para pigmentos. Sugere-se a obtenção de populações F2 dos híbridos 12, 25 e 27 visando a obtenção de novas cultivares de alfaces biofortificadas vermelhas e verdes, para os segmentos de alface crespa, americana, mini e romana. mundial saúde Lactuca L. L genético Assim BG Biofort hibridações 2 experimentais 3 linhagens, (linhagens comercias 1 UFU189#3#4#1 UFU189341 189#3#4#1 189 4 (UFU-189#3#4# UFU66#4#2 UFU6642 UFU66 (UFU66#4# UFU-189#3#4#1 produtividade carotenoide. carotenoide . carotenoide) BIOFORT1 Sugerese Sugere F verdes crespa americana romana UFU189 UFU189#3#4# UFU18934 189341 189#3#4# 18 (UFU-189#3#4 UFU66#4# UFU664 UFU6 (UFU66#4 UFU-189#3#4# UFU18 UFU189#3#4 UFU1893 18934 189#3#4 (UFU-189#3# UFU66#4 (UFU66# UFU-189#3#4 UFU1 UFU189#3# 1893 189#3# (UFU-189#3 UFU66# (UFU66 UFU-189#3# UFU189#3 189#3 (UFU-189# (UFU6 UFU-189#3 UFU189# 189# (UFU-189 (UFU UFU-189# (UFU-18 UFU-189 (UFU-1 UFU-18 (UFU- UFU-1 UFU-

Resumen Marco contextual: La implantación de la Práctica Basada en la Evidencia (PBE) es esencial para la calidad de los cuidados de enfermería. Las acciones emprendidas por los líderes formales son esenciales para implantar la PBE, y es fundamental conocer sus percepciones. Objetivo: Conocer las percepciones de los líderes formales de enfermería sobre la PBE. Metodología: Estudio exploratorio descriptivo con enfoque cualitativo. Se realizaron entrevistas semiestructuradas a 17 líderes de tres hospitales portugueses. Los datos se analizaron mediante análisis de contenido y el programa MAXQDA Analytic Pro 2022. Se garantizaron los presupuestos éticos. Resultados: Surgieron dos temas con sus respectivas categorías − Conocimiento sobre la PBE (Concepto de la PBE, Impacto de la PBE en los resultados sanitarios y Autopercepción del conocimiento sobre la PBE); Papel en la aplicación de la PBE (comportamientos y características). Conclusión: Los líderes describieron su percepción del concepto de PBE y la relación del Impacto de la PBE con los resultados sanitarios, la necesidad de conocimientos y su inversión formativa, así como su papel en este proceso. Los futuros programas de formación deberían aplicarse en este ámbito.

Resumo Enquadramento: A implementação da Prática Baseada na Evidência (PBE) é crucial para a qualidade dos cuidados de enfermagem. As ações desenvolvidas pelos líderes formais são essenciais para implementar a PBE, tornando-se determinante conhecer as suas perceções. Objetivo: Conhecer as perceções de líderes formais de enfermagem sobre a PBE. Metodologia: Estudo descritivo exploratório com abordagem qualitativa. Foram realizadas entrevistas semiestruturadas a 17 líderes de três hospitais portugueses. Os dados foram analisados através de análise de conteúdo e do software MAXQDA Analytics Pro 2022. Salvaguardados os pressupostos éticos. Resultados: Emergiram dois temas com respetivas categorias − Conhecimento sobre PBE (Conceito de PBE, Impacto da PBE nos resultados em saúde e Autoperceção do conhecimento sobre PBE); Papel na implementação da PBE (comportamentos e caraterísticas). Conclusão: Os líderes descreveram a sua perceção sobre o Conceito de PBE e a relação do Impacto da PBE com resultados em saúde, a necessidade de conhecimento e o seu investimento formativo, bem como o seu papel neste processo. Futuros programas formativos deverão ser implementados nesta área.

Abstract Background: The implementation of Evidence-Based Practice (EBP) is critical to the quality of nursing care. Formal leaders play a crucial role in developing interventions to implement EBP, so it is important to understand their perceptions. Objective: To examine formal nurse leaders’ perceptions of EBP. Methodology: Descriptive exploratory study with a qualitative approach. Semi-structured interviews were conducted with 17 leaders from three Portuguese hospitals. Data were analyzed using content analysis and MAXQDA Analytics Pro 2022 software. All ethical principles were observed. Results: Two themes and their categories emerged − Knowledge of EBP (Concept of EBP, Impact of EBP on health outcomes, and Self-perception of the knowledge about EBP) and Role in EBP implementation (behaviors and characteristics). Conclusion: The leaders described their perception of the concept of EBP and the association between the impact of EBP and health outcomes, the need for knowledge, and the investment in training, as well as their role in this process. Future training programs should be implemented in this area.

Resumen Marco contextual: Se ha reportado la participación de distracciones en errores clínicos y fallos de procedimiento. Objetivo: Analizar las distracciones del personal de enfermería durante la preparación y administración de fármacos de alto riesgo. Metodología: Estudio transversal desarrollado en una unidad de cuidados intensivos y una unidad de hospitalización. Se observaron distracciones del personal de enfermería durante el proceso de medicación a través de dos listas de control. La muestra fue seleccionada por conveniencia (abril-mayo 2019). Los datos cuantitativos se analizaron mediante estadística descriptiva (IBM SPSS Statistics, versión 24.0). Los datos cualitativos se analizaron mediante análisis de contenido. Resultados: Hubo 137 distracciones en 193 procesos de medicación. La mayoría de las distracciones fueron iniciadas por otros miembros del equipo sanitario a través de conversaciones. La mayoría se produjeron en la fase de preparación y fueron negativas y se gestionaron mediante la estrategia multitarea. Conclusión: Las distracciones durante el proceso de medicación se referían principalmente a las comunicaciones profesionales y sociales. La importancia de esas distracciones variaba en función de la fase del proceso.

Resumo Enquadramento: Estudos indicam que as interrupções contribuem para erros clínicos e falhas em procedimentos. Objetivo: Analisar as interrupções vivenciadas pelos enfermeiros durante a preparação e administração de medicamentos de alto risco. Metodologia: Foi realizado um estudo transversal numa unidade de cuidados intensivos e numa unidade de internamento. As interrupções vivenciadas pelos enfermeiros durante o processo de medicação foram observadas com a ajuda de duas checklists. A amostra foi selecionada por conveniência em abril e maio de 2019. Os dados quantitativos foram analisados através de estatística descritiva no programa IBM SPSS Statistics, versão 24.0, enquanto os dados qualitativos foram tratados por meio da análise de conteúdo. Resultados: Observaram-se 137 interrupções em 193 processos de medicação. A maioria das interrupções foi iniciada por outros membros da equipa de cuidados de saúde por meio de conversas. Estas interrupções foram maioritariamente prejudiciais e ocorreram durante a fase de preparação. A estratégia multitarefa foi utilizada para as gerir. Conclusão: As interrupções ocorridas durante o processo de medicação eram maioritariamente associadas com comunicações profissionais e sociais. A sua relevância diferiu consoante a fase do processo.

Abstract Background: Interruptions have been reported to contribute to clinical errors and procedural failures. Objective: To analyze the interruptions experienced by nurses during the preparation and administration of high-risk medications. Methodology: A cross-sectional study was conducted in an intensive care and inpatient unit. The interruptions experienced by nurses during the medication process were observed through two checklists. The sample was selected by convenience in April-May 2019. Descriptive statistics was used to analyze quantitative data in IBM SPSS Statistics software, version 24.0, while content analysis was used to analyze qualitative data. Results: In 193 medication processes, there were 137 interruptions. Other members of the healthcare team initiated most interruptions through conversations. These interruptions were mostly negative and occurred during the preparation phase. The multitasking strategy was used to manage them. Conclusion: Interruptions during the medication process were primarily associated with professional and social communications. The impact of these interruptions varied depending on the phase of the process.

Die prosesse wat gebruik word om onroerende eiendom van verkoper na koper in Suid-Afrika oor te dra, is verouderd en nie outomaties nie. Dokumente in papierformaat verhoog die risiko vir bedrog en wanvoorstellings. Hierdie artikel oorweeg die implementering van 'n wolkgebaseerde, gesentraliseerde databasis met eiendomsaanvoerkettingvennote, om deursigtigheid, omkeertyd en sekuriteit te verbeter, terwyl die risiko en koste geminimaliseer word. Die digitalisering van eiendomsprosesse sou lei tot die dematerialisering van die titelbewys, en nie bloot outomatisering van die papiergebaseerde stelsel nie. Die kwalitatiewe navorsing was verkennend, en halfgestruktureerde onderhoude is gevoer met rolspelers betrokke by eiendomsoordragte in Suid-Afrika. Dit het die Suid-Afrikaanse Reserwebank, die Departement van Landbou, Grondhervorming en Landelike Ontwikkeling, die Prokureursorde van Suid-Afrika, verbandopstellers, Die Bankvereniging, die Departement van Binnelandse Sake, die registrateur van die aktekantoor, die landmeter-generaal, munisipaliteite, sagtewaremaatskappye, die Nasionale Tesourie, Suid-Afrika se Sentrale Sekuriteitebewaarplek, sowel as die Tshwane Munisipaliteit se Raad, ingesluit.

The processes used to transfer immovable property from a seller to a buyer in South Africa are outdated and not digitised. Paper-based documents increase the risk of fraud and misrepresentation. This study considered the implementation of a cloud-based centralised database for property supply chain partners to enhance transparency, turnaround times, and security while minimising risks and costs. Digitalised property processes would result in title deeds' dematerialisation and not merely the automation of the paper-based system. This qualitative study was exploratory, and semi-structured interviews were conducted with role-players involved with property transfers in South Africa. These included the South African Reserve Bank, the Department of Agriculture, Land Reform and Rural Development, the Law Society of South Africa, mortgage originators, The Banking Association, the Department of Home Affairs, the Registrar of each Deeds Office, the Surveyor General's office, municipalities, software companies, National Treasury, South Africa's Central Securities Depository (Strate Ltd), and the Tshwane Mettropolitan Municipal Council.

Abstract Introduction: The master's degree program in nursing offered by the Universidad Autónoma de Sinaloa through the Culiacan School of Nursing contemplates that graduates should develop competencies and provide answers to problems related to nursing care. Objective: To describe experiences of graduates of the master's degree in nursing from the Culiacan School of Nursing. Methodology: Qualitative, descriptive, phenomenological, and interpretative study using the technique of in-depth interviews, supported by a semi-structured guide and with informed consent, applied to six graduates of the master's degree in nursing. The data were processed through thematic content analysis by De Souza Minayo in 3 stages: ordering and open coding, data classification, and horizontal and cross-sectional analysis, with which a category and two subcategories were obtained. The final analysis was carried out through an inflection from empirical to theoretical and vice versa. Results: In the experiences lived by the graduates, emotions experienced during the development of research competencies were identified; these emotions affected their cognitive process by living with stress, despair, fear, or depression, and these emotions led them to be competent in research, database search, use of software, and publishing skills. Conclusions: After their experiences in the master's degree program in nursing, the graduates showed that they had lived emotions that affected their mental health, facing physiological and emotional problems derived from the stress experienced. As for their research skills, they reported being able to search databases, perform critical analysis, and even publish a scientific article.

Abstrato Introdução: O programa de mestrado em enfermagem oferecido pela Universidade Autônoma de Sinaloa, por meio da Faculdade de Enfermagem de Culiacán, contempla que os graduados desenvolvam competências que respondam aos problemas relacionados ao cuidado de enfermagem. Objetivo: Descrever as experiências da egressa do mestrado em enfermagem da Faculdade de Enfermagem de Culiacán. Metodologia: Estudo qualitativo, descritivo, fenomenológico e interpretativo, técnica: entrevistas em profundidade, com apoio de roteiro semiestruturado, sob consentimento informado a seis egressos do programa de mestrado em enfermagem. Dados processados através da análise de conteúdo temática de Souza Minayo em 3 etapas: ordenação e codificação aberta, classificação dos dados, análise horizontal e transversal, obtenção de uma categoria e duas subcategorias, análise final através da inflexão do empírico para o teórico e vice-versa. Resultados: Nas experiências vividas pelos egressos foram identificadas emoções vivenciadas durante o desenvolvimento das competências de pesquisa, onde as emoções afetaram seu processo cognitivo ao conviver com estresse, desespero, medo ou depressão e nas competências de pesquisa os levaram a serem competentes, realizar banco de dados pesquisa, uso de software e capacidade de publicar. Conclusões: Os egressos evidenciaram após suas vivências no mestrado em enfermagem que vivenciaram emoções que afetaram sua saúde mental, ao enfrentarem problemas fisiológicos e emocionais derivados do estresse vivenciado, para o desenvolvimento de habilidades de pesquisa que relataram ser capazes de realizar uma busca em bases de dados, uma análise crítica, até a publicação de um artigo científico.

Resumen Introducción: El programa de maestría en enfermería que oferta la Universidad Autónoma de Sinaloa a través de la Facultad de Enfermería Culiacán, contempla que los egresados desarrollen competencias, que den respuesta a problemas relacionados con el cuidado enfermero. Objetivo: Describir las experiencias del egresado de la maestría en enfermería de la Facultad de Enfermería Culiacán. Metodología: Estudio cualitativo, descriptivo, fenomenológico e interpretativo, empleando la técnica de entrevistas a profundidad, con apoyo de guía semiestructurada, bajo consentimiento informado a seis egresados del programa de maestría en enfermería. Datos procesados a través de análisis de contenido tipo temático por de Souza Minayo en 3 etapas: ordenamiento y codificación abierta, clasificación de datos, análisis horizontal y transversal, obteniendo una categoría y dos subcategorías, análisis final a través de inflexión de empírico a teórico y viceversa. Resultados: En las experiencias vividas por los egresados se identificaron emociones vividas durante el desarrollo de competencias en investigación, donde las emociones afectaron su proceso cognitivo al vivir con estrés, desesperación, temor o depresión y en competencias en investigación los llevaron a ser competentes, al realizar búsqueda en bases de datos, uso de software, y habilidad para publicar. Conclusiones: Los egresados evidenciaron tras sus experiencias en la maestría en enfermería haber vivido emociones que afectaron su salud mental, al enfrentar problemas fisiológicos y emocionales derivados del estrés experimentado, al desarrollo de competencias en investigación refirieron ser capaces de realizar una búsqueda en bases de datos, un análisis crítico, hasta lograr publicar un artículo científico.