Resumen Introducción: Las infecciones del torrente sanguíneo asociadas a catéter (ITS/AC) son eventos prevenibles y potencialmente fatales, comunes en el contexto del cuidado de pacientes críticos. A mediados de 2018 se presentó un incremento en la tasa de incidencia de ITS/AC en una institución colombiana de alta complejidad, obligando a realizar intervenciones inmediatas para lograr una reducción de estas cifras. Objetivo: Evaluar la efectividad del método de mejoramiento continuo de la calidad (MCC) para la reducción de la tasa de incidencia de ITS/AC en un hospital universitario en Bogotá, Colombia. Métodos: Estudio longitudinal, prospectivo, en el que se implementa una intervención multifacética siguiendo la metodología de MCC. El proyecto se desarrolló en un hospital universitario de alta de complejidad de Bogotá, Colombia, entre julio de 2018 y diciembre de 2019. Se realizaron consecutivamente un análisis de causa raíz, priorización de factores contribuyentes, recuperación de ideas de mejora, construcción de la estrategia y priorización del plan de implementación. Resultados: El método de MCC permitió la detección de zonas susceptibles de presentación de errores en la inserción de catéteres y su mantenimiento en la Institución, además permitió priorizar las áreas por intervenir mediante ciclos consecutivos de prueba para las ideas de mejora. Se logró la reducción y sostenibilidad de la ITS/AC asociada a inserción luego de tres meses de iniciar las intervenciones, logrando valores de cero. La implementación de ideas de mejora dirigidas a reducir los eventos relacionados con el mantenimiento de catéteres también logró reducirlos a cero hasta la terminación del periodo de observación de este estudio. Conclusiones: Es factible implementar MCC en contextos como el aquí presentado para reducir de manera eficiente las ITS/AC.

Abstract Introduction: Central Line-Associated Bloodstream Infections (CLABSI) are preventable and potential fatal events, frequent in critical patient care. By mid-2018 an increase was noted in the incidence rate of CLABSI at a high complexity institution in Colombia, demanding immediate interventions to lower those numbers. Objective: To assess the effectiveness of the continuous quality improvement methodology (CQI) to lower the incidence rate of CLABSI at a university hospital in Bogotá, Colombia. Methods: Longitudinal, prospective study implementing a multifaceted intervention in accordance with the CQI methodology. The project was developed at a high complexity university hospital in Bogotá, Colombia, between July 2018 and December 2019. A root cause analysis was consecutively conducted prioritizing contributing factors, gathering ideas for improvement, building a strategy and prioritizing the implementation plan. Results: The CQI methodology enabled the identification of areas susceptible of catheter insertion and management errors at the institution; additionally, it allowed for the prioritization of the areas requiring intervention through consecutive test cycles for improvement ideas. The reduction and sustainability of insertion-related CLABSI was accomplished three months after the start of the interventions, achieving a zero value. The implementation of improvement ideas aimed at reducing the events associated with catheter maintenance was also able to reduce the incidence to zero, until the end of the period of observation of the study. Conclusions: It is feasible to implement CQI in settings similar to the one herein described, in order to efficiently reduce CLABSIs.

ABSTRACT Among the problems of physical degradation of soils, compaction and densification are the main ones, and these cause strong decreases in subsequent crop yields. The degradation of the soil structure is a problem today in Cuba, which can occur in all soils and at all levels of livestock production. Taking into account the above, this document aims to expose the main changes produced in the internal structure of soils as a result of its degradation. Soil compaction, due to the collapse or reduction of pore spaces, is the most common cause of physical restriction for root growth and development. As an aggravating factor, the degradation of the soil structure is commonly considered the most difficult type of land degradation to locate and reconvert; the reason is that this degradation is a subsurface phenomenon. Finally, it is about establishing some possible solutions to this problem, among which the main method of restoring the porosity of the limiting layers of root growth and that must take into account the chemical and physical factors of the soil, the species, the type of soil and the degree of degradation of the species that are proposed to restore, the use of plowing v+ harrow or plowing alone is the most efficient method for the recovery of pastures of the species.

RESUMEN Entre los problemas de la degradación física de los suelos, la compactación y el adensamiento son los principales, y estos causan fuertes decrementos en los rendimientos posteriores de los cultivos de los mismos. La degradación de la estructura del suelo es un problema hoy en Cuba, que puede ocurrir en todos los suelos y a todos los niveles de producción pecuaria. Teniendo en cuenta lo anterior, este documento pretende exponer los principales cambios producidos en la estructura interna de los suelos como resultado de su degradación. La compactación del suelo, debido al colapso o disminución de los espacios de poros, es la causa más común de restricción física para el crecimiento y desarrollo de las raíces. Como agravante, la degradación de la estructura del suelo es comúnmente considerada como el tipo de degradación de tierras más difícil de localizar y reconvertir; la razón es que ésta degradación es un fenómeno subsuperficial. Finalmente se trata de establecer algunas posibles soluciones a dicha problemática, entre las cuales el método principal de restauración de la porosidad de las capas limitantes del crecimiento de las raíces y que se deben tener en cuenta los factores químicos y físicos del suelo, la especie, el tipo de suelo y el grado de degradación de las especies que se proponen restaurar, el uso de la aradura + grada o de la aradura sola resulta el método más eficiente para la recuperación de pastizales de las especies

Abstract In commercial soursop orchards in the state of Nayarit, Mexico, trees with apparent symptoms of root rot are observed, without the causal agent being known. Therefore, the objective of the research was to identify the fungi that cause soursop root rot. From January 2020 to January 2021, in orchards of Compostela and San Blas, Nayarit, from two trees with symptoms of possible root rot, root sections with rot were collected. Microorganisms associated with the disease were isolated and identified to carry out pathogenicity tests in one-year-old soursop trees. Fusarium falciforme and Lasiodiplodia theobromae were identified as root pathogens, both fungi are reported for the first time in this fruit tree and for Mexico.

Resumen En huertos comerciales de guanábana del estado de Nayarit, México, se observan árboles con síntomas aparentes por pudrición radicular, sin que se conozca el agente causal. Por lo que, el objetivo de la investigación fue, identificar a los hongos causantes de la pudrición de raíz de guanábana. De enero de 2020 a enero de 2021, en huertos de Compostela y San Blas, Nayarit, de árboles con síntomas por posible pudrición radicular, se recolectaron secciones de raíz con pudrición. Se aislaron e identificaron a los microorganismos asociados a la enfermedad para realizar las pruebas de patogenicidad en árboles de guanábana de un año de edad. Se identificó a Fusarium falciforme y a Lasiodiplodia theobromae como patógenos de raíz, ambos hongos son reportados por primera vez en este frutal y para México.

Resumen La propuesta tiene como objeto el estudio del concepto "libre desarrollo de la personalidad" y sus implicaciones jurídicas como principio objetivo y derecho subjetivo intrínsecamente relacionado con los derechos y la dignidad humanos, así como el análisis de la reciente crisis axiológica y la banalización de las libertades públicas en el Estado constitucional de derecho. Se emplean los métodos analítico, crítico-valorativo e histórico, entre otros, sin soslayar la dogmática constitucional, como base de la teoría de la Constitución. Se concluye que el concepto del libre desarrollo de la personalidad no es un derecho absoluto, cuyas limitantes se prescriben en las Constituciones, de las cuales se toman como referentes la alemana y la española. Considerados paradigmáticos los derechos fundamentales, se contrasta su actual proliferación desmesurada y su vaciamiento axiológico que los identifica y esencializa. Lo anterior se asume como la principal causa de la crisis por la que atraviesan, que produce el fenómeno de la desconstitucionalización. Asimismo, se concluye que las libertades se deben concebir y, ejercer racionalmente con mesura, pero, sobre todo, con responsabilidad.

Abstract This proposal intends to study the concept of "self-determination" and its legal implications as an objective principle and subjective right intrinsically related to human rights and human dignity. It will also examine the recent axiological crisis and trivialization of public freedoms in the constitutional rule of law. Analytical, critical-evaluative, historical, and other methods are used without ignoring constitutional dogmatics as the basis of the theory of the Constitution. It is concluded that self-determination is not an absolute right whose limitations are prescribed in the constitutions, taking the German and Spanish constitutions as references. Regarded as paradigmatic, we contrast the disproportionate proliferation of fundamental rights and their axiological emptying, which characterizes and essentializes them. This is viewed as the root cause of the crisis they are going through, producing the phenomenon of deconstitutionalization. Thus, freedoms must be conceived and exercised rationally, measuredly, and, above all, responsibly.

Resumo Este projeto teve como objetivo identificar os impactos dos modelos de negócios atuais (agilidade de negócios) e dos principais controles de produtividade (KPIs), mais relevantes em um subsetor de pequenos restaurantes franqueados na Costa Rica (2020-2023). Para o qual se desenvolve uma investigação qualitativa, com entrevistas a 31 gestores de pequenos restaurantes que utilizam franquias nacionais e estrangeiras, para determinar diversas subcategorias de análise e estabelecer denominadores comuns que permitiram a superação da pandemia e a estruturação de uma análise de inclusão e exclusão, para priorizar os aspectos críticos de sucesso, a análise das causas, consequências e efeitos utilizando o método Root Cause para propor melhorias duradouras, sendo a principal constatação: a rigidez do modelo de franquia, a limitada agilidade do negócio e o uso de acesso limitado a maior tecnologia, principalmente devido ao custo e ao conhecimento, dificultou o enfrentamento do Cisne Negro da Pandemia por COVID-19).

Résumé Ce projet visait à identifier les impacts des modèles commerciaux actuels (agilité commerciale) et des contrôles clés de productivité (KPI), les plus pertinents dans un sous- secteur des petits restaurants franchisés au Costa Rica (2020- 2023). Pour lequel une enquête qualitative est développée, avec des entretiens avec 31 gérants de petits restaurants qui utilisent des franchises nationales et étrangères, pour déterminer diverses sous-catégories d’analyse et établir des dénominateurs communs qui nous ont permis de surmonter la pandémie et la structuration d’une analyse d’inclusion et d’exclusion, pour prioriser les aspects critiques du succès, l’analyse des causes, conséquences et effets selon la méthode Root Cause pour proposer des améliorations durables, le principal constat étant : la rigidité du modèle de franchise, l’agilité commerciale limitée et l’utilisation d’un accès limité L’essor de la technologie, principalement en raison du coût et des connaissances, a rendu plus difficile la lutte contre le cygne noir de la pandémie por COVID-19).

Abstract This project aimed to identify the impacts of current business models (business agility) and key productivity controls (KPIs), most relevant in a subsector of small franchised restaurants in Costa Rica (2020-2023). For which a qualitative investigation is developed, with interviews with 31 small restaurant managers who use national and foreign franchises, to determine various subcategories of analysis and establish common denominators that allowed us to overcome the pandemic and the structuring of an analysis of inclusion and exclusion, to prioritize the critical aspects of success, the analysis of the causes, consequences and effects using the Root Cause method to propose long-lasting improvements, the main finding being: the rigidity of the franchise model, the limited business agility, and the use Limited access to greater technology, mainly due to cost and knowledge, made it more difficult to face the Black Swan of the Pandemic por COVID-19).

Resumen Este proyecto buscó identificar los impactos de los actuales modelos de negocio (agilidad empresarial) y los controles clave de productividad (KPIs) más relevantes en un subsector de restaurantes pequeños franquiciados en Costa Rica (2020- 2023), por lo que se realizó una investigación cualitativa con entrevistas a 31 gerentes de restaurantes pequeños, usuarios de franquicia nacional y extranjera, para determinar así diversas subcategorías de análisis y establecer con esto denominadores comunes que permitieron sobrepasar la pandemia y la estructuración de un análisis de inclusión y exclusión, asimismo, para jerarquizar los aspectos críticos de éxito, el análisis de las causas, consecuencias y efectos mediante el método de Causa-Raíz para proponer mejoras de larga duración. Este es el principal hallazgo: la rigidez del modelo de franquicia, la limitada agilidad empresarial y el uso limitado de mayor tecnología, principalmente por costo y conocimiento, que hicieron más difícil afrontar el “Cisne negro” de la Pandemia por COVID-19).

Abstract A compression or interruption of the oculosympathetic pathway is generally the cause of Horner syndrome. Different etiologies are usually considered, either in the central or in the peripheral nervous system. Horner syndrome due to a herniated cervical or dorsal disc seems to be an excedingly rare event, which is the diagnosis of the presented case. The patient is a 59-year-old woman with sudden onset of dorsal pain and right unilateral ptosis and slight miosis, along with numbness in the third, fourth and fifth fingers of the right hand, due to a D1-D2 herniated disc with associated spinal nerve root compression. An extensive investigation ruled out other possible etiologies. In conclusion, the differential diagnosis of patients presenting with Horner syndrome should include upper thoracic disk herniation.

Resumo A compressão ou bloqueio da via óculossimpática é a causa principal de síndrome de Horner. Várias etiologias podem geralmente ser consideradas, quer no sistema nervoso central, quer no periférico. A síndrome de Horner devida a uma hérnia discal cervical ou dorsal constitui um evento particularmente raro, sendo o diagnóstico do caso apresentado. Este trata uma doente de 59 anos com um quadro súbito de dorsalgia e ptose unilateral direita, devidas a uma hérnia discal ao nível de D1-D2. Foi conduzida uma investigação extensiva para excluir outras possíveis etiologias. Em conclusão, o diagnóstico diferencial de doentes com síndrome de Horner deverá incluir a hérnia discal torácica superior.

Reliable cable systems are of utmost importance for electricity distribution- and grid networks. Cable terminations are key components of cable systems but also contribute significantly towards unwanted cable failures and power outages. Although well-developed standards exist in the electricity industry, it is not always possible to execute the most effective tests at power frequency due to financial or logistical reasons. Hence, withstand voltage- and Partial Discharge tests at power frequency are only conducted at a low percentage of electricity utilities. This paper presents the evaluation of unconventional partial discharge fault detection techniques and Very Low Frequency Tan Delta measurements on different cable termination defects. Five artificial cable termination defects were created on single core 6.35/11kV cross linked polyethylene cables, with one "defect-free" termination and five defective ones. Power frequency voltage was applied at increased voltage steps to initiate aging acceleration due to partial discharge. This resulted in insulation deterioration and eventual termination failure. Different data sets were obtained from the Very Low Frequency Tan Delta measurements as well as for the unconventional Partial Discharge measurements. The sequence of failures is compared between the two data sets and evaluated against the root cause failures of the terminations as evaluated by an industry expert. High Frequency Current Transformer and capacitive coupling sensors were used together with an advanced partial discharge data acquisition system. Partial discharge analysis was done to relate the specific on-line trending data with termination failure. Good correlation was found between the two data sets which supports the utilization of unconventional partial discharge measurement for cable acceptance testing. It would be beneficial for the electricity industry to consider unconventional Partial Discharge technology as a suitable alternative for on-site acceptance tests for cable terminations.

ABSTRACT Copper is an essential element for beet; however, it needs to be made available in adequate quantities since this nutrient, which is a heavy metal, can cause toxicity to plants and/or humans. This study aimed to evaluate the agronomic performance of beet fertigated with copper in a semi-arid region of Brazil. Two experiments were carried out in complete randomized blocks, with five treatments (0, 1.5, 3.0, 4.0, and 6.0 kg ha-1 of copper) and four replicates. In 2019, the copper content was within the adequate range for beet. In 2021, fertigation with copper above 0.8 kg ha-1 led to contents in the phytotoxicity range, which may explain the reduction in total and tuberous root dry mass accumulation. Fertilization with copper increased marketable yield in 2019 (17.32 t ha-1 at the dose of 3.9 kg ha-1 of copper), while, in 2021, there was no fit of the equations. Copper fertilization altered the tuberous root pH but did not influence the other tuberous root quality components. Plants fertilized with copper accumulated more copper in the leaves than in the tuberous root, with a maximum accumulation of 6.0 kg ha-1 of copper in 2019 and 2021. Fertilization with 3.9 kg ha-1 suits soils with low copper content. however nutrient metal andor or humans semiarid semi arid Brazil blocks 0, 0 (0 15 1 5 1.5 30 3 3.0 40 4 4.0 60 6 6. ha1 ha ha- replicates 2021 08 8 0. 201 17.32 1732 17 32 (17.3 39 9 3. copper, , while equations components ( 1. 4. 202 20 17.3 173 (17. 2 17. (17 (1

RESUMO O cobre é um elemento essencial à beterraba, no entanto, é necessário disponibilizá-lo em quantidade adequada uma vez que esse nutriente, que é um metal pesado, pode causar toxicidade às plantas e, ou, ao ser humano. Esta pesquisa teve como objetivo avaliar o desempenho agronômico de beterraba fertirrigada com cobre em uma região semiárida do Brasil. Foram conduzidos dois experimentos em delineados em blocos casualizados ao acaso, com cinco tratamentos (0, 1.5, 3.0, 4.5 e 6.0 kg ha-1 de cobre) e quatro repetições. Em 2019, o teor de cobre estava dentro da faixa adequada para a beterraba. Em 2021, a fertirrigação com cobre acima de 0.8 kg ha-1 proporcionou teores na faixa de fitotoxicidade, o que pode explicar a redução no acúmulo de massa seca total e de raízes tuberosas. A adubação com cobre aumentou a produtividade comercial em 2019 (17.32 t ha-1 na dose de 3.9 kg ha-1 de cobre) enquanto que, em 2021, não houve modelo ajustado. A adubação alterou o pH da raiz tuberosa, mas não influenciou nos demais atributos de qualidade pós-colheita da raiz tuberosa. Plantas adubadas com cobre acumularam mais cobre nas folhas do que na raiz tuberosa, com máxima acumulada em 2019 e em 2021, na dose 6.0 kg ha-1 de cobre. A adubação com 3.9 kg ha-1 é adequada para solos com baixo teor de cobre. entanto disponibilizálo disponibilizá lo nutriente pesado ou humano Brasil acaso 0, 0 (0 15 1 5 1.5 30 3 3.0 45 4 4. 60 6 6. ha1 ha ha- repetições 2021 08 8 0. fitotoxicidade tuberosas 201 17.32 1732 17 32 (17.3 39 9 3. ajustado tuberosa póscolheita pós colheita ( 1. 202 20 17.3 173 (17. 2 17. (17 (1

ABSTRACT: Endodontic treatment requires detailed knowledge of root morphology. In maxillary premolars, variability in root number and root canal system (RCS) morphology has been reported. The most frequent cause of endodontic failure is non treated canals. Therefore, a better understanding of RCS morphology and its variations is crucial for endodontic treatment. The aim of this research was to describe the frequency of root number and SCR morphology in maxillary premolars, by means of Cone Beam Computed Tomography (CBCT) examinations in a Chilean population. A cross-sectional descriptive observational study was carried out in which the morphology of the root canal system of maxillary first and second premolars was observed through CBCT examinations, grouping them according to the Vertucci Classification. A total of 228 CBCT examinations were observed, which included 268 upper first premolars and 233 maxillary second premolars. It was determined that in maxillary first premolars 56.3 % presented one root, 43.3 %, being more frequent a SCR type IV morphology. In maxillary second premolars, the presence of one root was determined in 95.7 % of the cases, being more frequent a SCR type I morphology. These results may be of interest to endodontists, since knowing the morphology of the RCS of maxillary premolars allows improving the planning of endodontic therapy performed on these teeth.

RESUMEN: El tratamiento endodóntico requiere detallados conocimientos sobre la morfología radicular. En premolares maxilares, se ha reportado variabilidad en el número de raíces y morfología del sistema de canales radiculares (SCR). La causa más frecuente de fracaso endodóntico son los conductos no tratados. Por lo tanto, una mejor compresión de la morfología del SCR y sus variaciones es crucial para el tratamiento endodóntico. El objetivo de esta investigación fue describir la frecuencia del número de raíces y morfología del SCR en premolares maxilares, mediante exámenes de tomografía computarizada Cone Beam (CBCT) en una población chilena. Se realizó un estudio observacional descriptivo de corte transversal donde se observó la morfología del sistema de canales radiculares de primeros y segundos premolares maxilares a través de exámenes CBCT, agrupándolos de acuerdo con la Clasificación de Vertucci. Se observaron 228 exámenes CBCT, donde se incluyeron 268 primeros premolares superiores y 233 segundos premolares maxilares. Se determinó que en primeros premolares maxilares un 56,3 % presentó una raíz, un 43,3 %, siendo más frecuente una morfología del SCR tipo IV. En los segundos premolares maxilares, se determinó la presencia de una raíz en el 95,7 % de los casos, siendo más frecuente una morfología del SCR tipo I. Estos resultados pueden ser de interés para endodoncistas, ya que conocer la morfología del SCR de premolares maxilares permite mejorar la planificación de la terapia endodóntica realizada en estos dientes.

Cleaning and shaping of the root canal are profoundly affected by the complexity of root and canal morphology. Undiscovered roots or canals may lead to a reduced prognosis of a treated tooth as hidden causative organisms and their by-products can cause re-infection. Most maxillary first molars have three roots, namely mesio-buccal (MB), disto-buccal (DB) and palatal (P). They can be separate or fused, with incidences varying between populations. Anomalies have also been documented that include single-rooted, double-rooted, four and even five-rooted teeth. Additional roots are mostly in the form of additional palatal roots and are known as either a radix mesiolingualis (RML) or radix distolingualis (RDL). This paper is the first of two giving an overview, focused on micro-CT, of available literature on various aspects of the root and canal morphology of the maxillary first permanent molar. The aim of this paper is to provide an overview of relevant aspects of the external root morphology in different populations. The content is supported by illustrative micro-CT images and case reports of rare morphological findings on maxillary first molars.

Resumo A contaminação dos recursos naturais com metais pesados liberados pelas siderúrgicas é a principal causa da poluição do solo e da água no Dargai Malakand, localizado no lado norte do Paquistão. Portanto, o presente estudo teve como objetivo determinar o teor de metais pesados em amostras de solo e água desta área. O cultivo de plantas silvestres (nove plantas nativas: Pteris vittata, Populus nigra, Eucalyptus camaldulensis, Persicaria maculosa, Arundo donax, Xanthium strumarium, Verbascum thapsus, Ricinus communis e Parthenium hysterophorus) foi testado quanto às suas capacidades de fitorremediação, que é um meio ambiente, geralmente utilizado, e abordagem de baixo custo para eliminar metais pesados de solos e águas poluídas. Amostras de solo, água e efluentes foram retiradas dos locais contaminados de sete siderúrgicas no distrito de Dargai, Malakand, e submetidas à análise de metais pesados. Com base no fator de bioconcentração (BCF) e fator de translocação (TF) calculados, o maior BCF para zinco foi registrado para as raízes de Pteris vittata (3,93), enquanto o menor valor foi observado para as folhas de Verbascum thapsus (0,306). A raiz de Pteris vittata apresentou o maior BCF para ferro (1,618), enquanto as folhas de Ricinus communis apresentaram o menor (0,023). O maior valor de BCF para cromo foi maior para raízes de Populus nigra (0,717), enquanto o menor valor foi registrado para folhas de Persicaria maculosa (0,031). Para os metais selecionados, Fe, Zn e Cr, os maiores TF foram registrados para Pteris vittata (0,988), Verbascum thapsus (0,944) e Xanthium strumairum (0,968), respectivamente. Portanto, recomenda-se que essas plantas sejam cultivadas próximas a siderúrgicas para recuperar metais pesados de efluentes industriais, solo poluído e água poluída.

Abstract The contamination of natural resources with heavy metals released from steel mills is the primary cause of soil and water pollution in the Dargai Malakand, located on the northern side of Pakistan. Therefore, the present study was aimed to determine the level of heavy metals in soil and water samples of this area. The wild plant growing (nine native plants: Pteris vittata, Populus nigra, Eucalyptus camaldulensis, Persicaria maculosa, Arundo donax, Xanthium strumarium, Verbascum thapsus, Ricinus communis and Parthenium hysterophorus) there were then tested for their phytoremediation capabilities which is an environmentally friendly, generally utilized, and low-cost approach to eliminate heavy metals from polluted soils and water. Soil, water, and effluent samples were taken from the contaminated sites of seven steel mills in Dargai District Malakand and subjected to heavy metals analysis. Based on bioconcentration factor (BCF) and translocation factor (TF) calculated, The highest BCF for zinc was recorded for Pteris vittata roots (3.93), while the lowest value was observed for Verbascum thapsus leaves (0.306). Pteris vittata root showed the highest BCF for iron (1.618), while Ricinus communis leaves showed the lowest (0.023). The highest BCF value for chromium was highest for Populus nigra roots (0.717), while the lowest value was recorded for Persicaria maculosa leaves (0.031). For the selected metals; Fe, Zn and Cr the highest TF were recorded for Pteris vittata (0.988), Verbascum thapsus (0.944) and Xanthium strumairum (0.968) respectively. Therefore, it is recommended that these plants should be grown near to steel mills to reclaim heavy metals from industrial effluent, polluted soil as well as from polluted water.

Resumo Bulbine natalensis Baker e Chorophytum comosum (Thunb.) Jacques são potenciais fontes medicinais para o tratamento de cânceres. A Leucemia Mieloide Crônica (LMC) é um distúrbio das células-tronco hematopoiéticas que é tratado com inibidores da tirosina quinase, mas frequentemente, causa recorrência da leucemia após a interrupção da terapia, portanto, requer um tratamento alternativo. Este estudo determinou o efeito anticancerígeno de extratos metanólicos e aquosos de folha, raiz e bulbo de B. natalensis e C. comosum na linhagem celular de leucemia mieloide humana crônica (K562) por ensaios de MTT, Hoechst bis-benzimida nuclear e anexina V. O extrato metanólico da raiz de B. natalensis e C. comosum apresentou alta citotoxidade de 8,6% e 16,7% respectivamente, na linhagem celular K562 com a concentração de 1,000 μg / ml. Perda morfológica da integridade da membrana celular causando degradação dos núcleos, citoplasma e encolhimento celular foi observada no extrato metanólico da raiz de ambas as plantas. Uma alta apoptose (p <0,0001) foi induzida nas células K562 por extratos de folhas e raízes de C. comosum em comparação com B. natalensis. Este estudo mostrou que ambas as plantas possuem efeito apoptótico contra leucemia mieloide in vitro que contribui para as propriedades anticâncer gerais de B. natalensis e C. comosum para justificar futuras aplicações terapêuticas contra câncer de sangue de LMC.

Abstract Bulbine natalensis and Chorophytum comosum are potential medicinal source for the treatment of cancers. Chronic myeloid leukaemia is a hematopoietic stem cells disorder treated by tyrosine kinase inhibitors but often cause recurrence of the leukaemia after cessation of therapy, hence require alternative treatment. This study determines the anti-cancer effect of leaf, root and bulb methanolic and aqueous extracts of B. natalensis and C. comosum in chronic human myelogenous leukaemia (K562) cell line by MTT, Hoechst bis-benzimide nuclear and annexin V stain assays. The root methanolic extract of B. natalensis and C. comosum showed a high cytotoxicity of 8.6% and 16.7% respectively on the K562 cell line at 1,000 μg/ml concentration. Morphological loss of cell membrane integrity causing degradation of the cell and fragmentation were observed in the root methanolic extract of both plants. A high apoptosis (p < 0.0001) was induced in the K562 cells by both leaf and root extracts of the C. comosum compared to the B. natalensis. This study shows both plants possess apoptotic effect against in vitro myelogenous leukaemia which contributes to the overall anti-cancer properties of B. natalensis and C. comosum to justify future therapeutic applications against chronic myelogenous leukaemia blood cancer.

ABSTRACT: In Brazil, urea is the most used nitrogen (N) fertilizer to improve forage production. However, their excessive use can cause environmental impacts through N losses, such as ammonia (NH3) volatilization. Therefore, the current study adjusted and estimated the NH3 volatilization from urea applied on tropical pastures in three rainfall conditions using mathematical models. Data were collected from Marandu grass (Brachiaria brizantha) fertilized with 50 kg N ha-1 during wet, intermediate, and dry conditions. Ammonia volatilization was measured in five semi-open chambers for 21 days. The linear, quadratic, exponential, Gompertz, Groot, and Richards models were tested for fitting and estimating the NH3 volatilization. The Gompertz, Groot, and Richards models generated predictions similar to the observed data, with a high determination coefficient, indicating a better fit of these equations to data, with precision and accuracy. However, the Groot model was selected due to the lowest root mean square error of prediction (0.29 % total N lost as NH3). The greatest N loss as NH3 volatilization occurred in the wet, followed by intermediate and dry conditions (20.2, 17.0, and 11.3 % total N lost as NH3, respectively). Therefore, nitrogen losses as NH3 volatilization after application of 50 kg N ha-1, as urea source, are altered according to the weather conditions, reaching 20% of N added in the wet rainfall period. The Groot model is recommended for fitting and estimating the NH3 volatilization from urea applied on Marandu grass pastures in the wet and dry rainfall conditions. ABSTRACT Brazil (N production However NH (NH3 Therefore Brachiaria brizantha 5 ha1 ha 1 ha- semiopen semi open 2 days linear quadratic exponential Gompertz data coefficient accuracy 0.29 029 0 29 (0.2 NH3. . NH3) 20.2, 202 20 (20.2 170 17 17.0 113 11 3 11. respectively. respectively respectively) ha1, 1, source period (NH 0.2 02 (0. 20.2 (20. 17. 0. (0 20. (20 ( (2

RESUMO: No Brasil, a ureia é o fertilizante nitrogenado mais utilizado para melhorar a produção de forragem. No entanto, seu uso excessivo pode causar impactos ambientais por meio de perdas de nitrogênio (N), como a volatilização da amônia (NH3). Portanto, o objetivo do presente estudo foi ajustar a volatilização de NH3 da ureia aplicada em pastos tropicais em três condições de chuva utilizando modelos matemáticos. Dados foram coletados de pastos de capim-marandu (Brachiaria brizantha) adubado com 50 kg N ha-1 em condições úmidas, intermediárias e secas. A volatilização da NH3 foi medida em cinco câmaras semiabertas durante 21 dias. Os modelos, linear, quadrático, exponencial, Gompertz, Groot e Richards foram testados para ajuste e estimativa da volatilização do NH3. Os modelos de Gompertz, Groot e Richards geraram predições semelhantes aos dados observados, com alto coeficiente de determinação, indicando um melhor ajuste dessas equações aos dados, com acurácia e precisão. No entanto, o modelo Groot foi selecionado devido ao menor erro quadrático médio das predições (0,29% de N total perdido como NH3). A maior volatilização de NH3 ocorreu em condições climáticas úmida, seguido por intermediária e seca (20,2; 17,0 e 11,3% de N total perdido como NH3, respectivamente). Portanto, as perdas de N como volatilização de NH3 após a aplicação de 50 kg N ha-1, como fonte de ureia, são alteradas de acordo com as condições climáticas, atingindo a 20% do N adicionado nas condições úmidas. O modelo Groot é recomendado para ajuste e estimativa da volatilização de NH3 da ureia aplicada em pastos de capim Marandu em condições úmidas e secas. RESUMO Brasil forragem entanto N, , (N) NH . (NH3) Portanto matemáticos capimmarandu marandu Brachiaria brizantha 5 ha1 ha 1 ha- secas 2 dias linear exponencial Gompertz observados determinação precisão 0,29% 029 0 29 (0,29 NH3) úmida 20,2 202 20 (20,2 170 17 17, 113 11 3 11,3 respectivamente. respectivamente respectivamente) ha1, 1, (N (NH3 0,29 02 (0,2 20, (20, 11, (NH 0,2 (0, (20 0, (0 (2 (

ABSTRACT Root-knot nematodes cause a significant reduction in the productivity of the gilo due to physiological stress. Incorporating organic residue into the soil has a positive effect in reducing nematode infestation. The aim of this study was to evaluate the effect of the addition of organic residue on the reproduction of Meloidogyne javanica and its role in the physiology of the gilo. One-litre pots, each containing one gilo seedling, were inoculated with 5000 eggs of M. javanica and the following treatments evaluated: 1) no inoculation and no residue (NIR); 2) NIR; 3) 12.5 g L-1 poultry manure; 4) 25 g L-1 cattle manure; 5) 20 g L-1 filter cake; 6) 5 g L-1 of the shoots of Tagetes patula; 7) 6.25 g L-1 of the shoots of Brassica oleracea var. capitata; 8) 20 mL L-1 vinasse and 9) 1 mL Abamectin-based commercial product (18 g L-1 a.i.). A randomised block design was used, with six repetitions. Gas exchange variables were evaluated 15, 30 and 45 days after inoculation with the nematode (DAI) and the vegetative and nematological variables at 60 DAI. The treatments that included cattle manure and filter cake afforded the best physiological rate and development in the gilo. The best results in controlling the nematode were given by the poultry manure, cattle manure and filter cake, reducing the reproduction of M. javanica by 41.76, 51.44 and 52.40%, respectively. Based on the results, poultry manure, cattle manure and filter cake are efficient in controlling M. javanica and show potential to be used in the integrated management of M. javanica in the gilo. Rootknot Root knot stress infestation Onelitre One litre pots seedling 500 M NIR (NIR) 2 3 125 12 12. L1 L L- 4 6 patula 7 625 6.2 var capitata 8 9 Abamectinbased Abamectin based 18 (1 a.i.. ai a.i. . i a.i.) repetitions 15 DAI (DAI 4176 41 76 41.76 5144 51 44 51.4 5240 52 40 52.40% respectively 50 (NIR 62 6. ( a.i 417 41.7 514 51. 524 52.40 41. 52.4 52.

Abstract: This study evaluated the stress distribution in the dentoalveolar and palatal bone structures during maxillary expansion in a 17-year-old male patient with bilateral cleft lip and palate (BCLP) using expanders with dental (HYRAX) and skeletal anchorage (MARPE). For the generation of the specific finite element models, cone-beam computed tomography was used, and the DICOM files were exported to Mimics 3-Matic (Materialise) and Patran (MSC Software) software. Three specific three-dimensional models were generated: A) HYRAX: conventional four-banded hyrax screw (9 mm); B) MARPE-DS: 3 miniscrews (1.8 mm diameter – 5.4 mm length) and four-banded dental anchorage; and C) MARPE-NoDS: 3 miniscrews without dental anchorage. Maxillary expansion was simulated by activating the expanders transversely 1 mm on the "X" axis. HYRAX resulted in higher levels of deformation predominantly in the dentoalveolar region. MARPE-DS showed stress in the dentoalveolar region and mainly in the center of the palatal region, at approximately 4,000 με. MARPE-NoDS exhibited evident stress only in the palatal region. High stress levels in the root anchoring teeth were observed for HYRAX and MARPE-DS. In contrast, MARPE-NoDS cause stress on the tooth structure. The stress distribution from the expanders used in the BLCP showed asymmetric expansive behavior. During the initial activation phase of expansion, the HYRAX and MARPE-DS models produced similarly high strain at the dentoalveolar structures and upper posterior teeth displacement. The MARPE-NoDS model showed restricted strain on the palate. Abstract 17yearold yearold 17 year old BCLP (BCLP (HYRAX MARPE. MARPE . (MARPE) conebeam cone beam 3Matic Matic Materialise (Materialise MSC Software software threedimensional three dimensional generated A fourbanded four banded 9 ( mm) B MARPEDS DS 1.8 18 8 (1. 54 5 4 5. length C MARPENoDS NoDS X "X axis 4000 000 4,00 με MARPEDS. DS. contrast structure behavior displacement (MARPE 1. (1 400 00 4,0 40 0 4,