Die prosesse wat gebruik word om onroerende eiendom van verkoper na koper in Suid-Afrika oor te dra, is verouderd en nie outomaties nie. Dokumente in papierformaat verhoog die risiko vir bedrog en wanvoorstellings. Hierdie artikel oorweeg die implementering van 'n wolkgebaseerde, gesentraliseerde databasis met eiendomsaanvoerkettingvennote, om deursigtigheid, omkeertyd en sekuriteit te verbeter, terwyl die risiko en koste geminimaliseer word. Die digitalisering van eiendomsprosesse sou lei tot die dematerialisering van die titelbewys, en nie bloot outomatisering van die papiergebaseerde stelsel nie. Die kwalitatiewe navorsing was verkennend, en halfgestruktureerde onderhoude is gevoer met rolspelers betrokke by eiendomsoordragte in Suid-Afrika. Dit het die Suid-Afrikaanse Reserwebank, die Departement van Landbou, Grondhervorming en Landelike Ontwikkeling, die Prokureursorde van Suid-Afrika, verbandopstellers, Die Bankvereniging, die Departement van Binnelandse Sake, die registrateur van die aktekantoor, die landmeter-generaal, munisipaliteite, sagtewaremaatskappye, die Nasionale Tesourie, Suid-Afrika se Sentrale Sekuriteitebewaarplek, sowel as die Tshwane Munisipaliteit se Raad, ingesluit.

The processes used to transfer immovable property from a seller to a buyer in South Africa are outdated and not digitised. Paper-based documents increase the risk of fraud and misrepresentation. This study considered the implementation of a cloud-based centralised database for property supply chain partners to enhance transparency, turnaround times, and security while minimising risks and costs. Digitalised property processes would result in title deeds' dematerialisation and not merely the automation of the paper-based system. This qualitative study was exploratory, and semi-structured interviews were conducted with role-players involved with property transfers in South Africa. These included the South African Reserve Bank, the Department of Agriculture, Land Reform and Rural Development, the Law Society of South Africa, mortgage originators, The Banking Association, the Department of Home Affairs, the Registrar of each Deeds Office, the Surveyor General's office, municipalities, software companies, National Treasury, South Africa's Central Securities Depository (Strate Ltd), and the Tshwane Mettropolitan Municipal Council.

Abstract Frailty, defined as an inappropriate response to stressful situations due to the loss of physiological reserve, was initially described in the elderly population, but is currently being identified in younger populations with chronic diseases, such as chronic kidney disease. It is estimated that about 20% of patients are frail at the time of kidney transplantation (KT), and there is great interest in its potential predictive value for unfavorable outcomes. A significant body of evidence has been generated; however, several areas still remain to be further explored. The pathogenesis is poorly understood and limited to the extrapolation of findings from other populations. Most studies are observational, involving patients on the waiting list or post-KT, and there is a scarcity of data on long-term evolution and possible interventions. We reviewed studies, including those with Brazilian populations, assessing frailty in the pre- and post-KT phases, exploring pathophysiology, associated factors, diagnostic challenges, and associated outcomes, in an attempt to provide a basis for future interventions. Frailty reserve population diseases disease 20 KT, KT , (KT) outcomes generated however explored observational postKT, postKT post longterm long term interventions pre phases pathophysiology factors challenges 2 (KT

Resumo A fragilidade, conceituada como a resposta inadequada a situações de estresse devido à perda da reserva fisiológica, foi descrita incialmente na população idosa, mas atualmente vem sendo identificada em populações mais jovens com doenças crônicas, como a doença renal crônica. Estima-se que cerca de 20% dos pacientes sejam frágeis no momento do transplante renal (TR), e há grande interesse pelo seu potencial valor preditor de desfechos desfavoráveis. Um número expressivo de evidências tem sido gerado, entretanto, ainda persistem várias áreas a serem mais exploradas. A patogênese é pouco conhecida e limitada à extrapolação dos achados de outras populações. A maioria dos estudos é observacional, envolvendo pacientes em lista ou após o TR, e há escassez de dados sobre a evolução em longo prazo e possíveis intervenções. Revisamos os estudos, incluindo aqueles com populações brasileiras, de avaliação de fragilidade nas fases pré e pós-TR, explorando a fisiopatologia, os fatores associados, os desafios do diagnóstico e os desfechos associados, na tentativa de fornecer embasamento para futuras intervenções. fisiológica idosa crônicas crônica Estimase Estima se 20 TR , (TR) desfavoráveis gerado entretanto exploradas observacional intervenções brasileiras pósTR, pósTR pós pós-TR fisiopatologia associados 2 (TR

Resumen El trabajo consiste en una reconstrucción histórico-geografía por medio de la memoria de las personas residentes de la selva, utilizando la expresión popular avizinhar en la consolidación del concepto de Extractivismo en la amazonia acreana. La metodología se basa en análisis bibliográficos, documentos, trabajo de campo, entrevistas e imágenes de dominio público. El extractivismo se desarrolló a lo largo de la historia en todo el mundo, con diferentes sesgos: como actividad que tiene su acción ligada a una extracción sin precedentes de elementos de la naturaleza, extracción sin la debida preocupación por los daños colaterales o la ausencia de tales elementos en la composición del ecosistema. Sin embargo, el presente trabajo procura presentar y conceptualizar el extractivismo a partir de la experiencia de la Amazonía, en particular el estado brasileño de Acre que, a pesar de cumplir con la etimología de la palabra, “retirada, recolección de materiales de la naturaleza”, trasciende la extracción depredadora, y se revierte en luchas por la conservación de la naturaleza y de las vidas humanas que habitan las selvas. Estas acciones fueron lidereadas por Chico Mendes a mediados de la década de 1980 a través de la organización sindical de los trabajadores forestales iniciada en la década de 1970.

Abstract The work consists of a historical-geographical reconstruction, using the popular orkers avizinhar in the consolidation of the orkers Extractivism in the Acrean Amazon. The methodology is based on bibliographic analysis, documents, fieldwork, interviews, public domain images. Extractivism developed throughout history throughout the world, with orkers biases: as na activity that has its action linked to na unprecedented extraction of elements of nature, extraction without due concern for orkers damage or the absence of such elements in the composition of the ecosystem. However, the orkers work seeks to orkers and conceptualize extractivism from the experience of the Amazon, in particular the Brazilian state of Acre that, despite complying with the etymology of the word, “withdrawal, collection of materials from nature”, transcends predatory extraction, and reverts into struggles for the conservation of nature and human lives that inhabit the forests. These actions were led by Chico Mendes in the mid-1980s through the union organization of forestry orkers initiated in the 1970s.

Resumo O trabalho consiste em uma reconstrução histórico-geográfica, utilizando a expressão popular avizinhar na consolidação do conceito de Extrativismo na Amazônia acreana. A metodologia baseia-se em análise bibliográfica, documentos, trabalho de campo, entrevistas e imagens de domínio público. O extrativismo desenvolveu-se ao longo da história em todo o mundo, com diferentes vieses: como uma atividade que tem sua ação ligada a uma extração sem precedentes de elementos da natureza, extração sem a devida preocupação com danos colaterais ou a ausência de tais elementos na composição do ecossistema. No entanto, o presente trabalho busca apresentar e conceituar o extrativismo a partir da experiência da Amazônia, em particular do estado brasileiro do Acre que, apesar de obedecer à etimologia da palavra, “retirada, coleta de materiais da natureza”, transcende o extrativismo predatório, e se reverte em lutas pela conservação da natureza e das vidas humanas que habitam as florestas. Essas ações foram lideradas por Chico Mendes em meados da década de 1980 por meio da organização sindical dos trabalhadores florestais iniciada na década de 1970.

Resumen: Las Reservas Ecológicas, son la base primordial para la subsistencia de la biodiversidad, desempeñan funciones cruciales para el mantenimiento de los ecosistemas, especialmente, en la preservación de los servicios ecosistémicos de regulación del clima y suministro de agua. Determinar la capacidad de acumulación del COS en tres ecosistemas de la Reserva Ecológica Arenillas (REAR) y la influencia que tienen con los factores de profundidad y el tipo de suelo, fue el objetivo de la investigación. Se partió de la hipótesis que dichos factores ejercen un efecto sobre las propiedades físicas-químicas del suelo, provocando la variabilidad del carbono. Se establecieron 14 unidades de muestreo para los tres ecosistemas; 1) Intervenido, 2) Bosque deciduo y 3) Bosque arbustal deciduo, se extrajo de cada unidad dos muestras de suelo a profundidades de (0-10 y 10-30 cm), dando un total de 28 muestras. Los resultados revelaron diferencias significativas entre los tres tipos de ecosistemas, permitiendo afirmar que, en la REAR, el contenido de COS es mayor en el ecosistema de Bosque deciduo, que en los denominados Bosque arbustal deciduo y la zona Intervenida. Así mismo, los suelos del tipo Alfisol y Entisol en el Bosque deciduo presentaron la mayor capacidad de almacenamiento de COS, siendo la capa superficial la de mayor acumulación en los tres ecosistemas. Se concluyó que las bajas precipitaciones, el aumento de la temperatura y la esporádica vegetación producida por actividades antrópicas, afectan el almacenamiento de COS, limitando la prestación de servicios ecosistémicos relacionados con la captura de carbono edáfico.

Abstract: Ecological Reserves are the primary basis for the subsistence of biodiversity, they play crucial roles in the maintenance of ecosystems, especially in the preservation of ecosystem services of climate regulation and water supply. The objective of the research was to determine the accumulation capacity of COS in three ecosystems of the Arenillas Ecological Reserve (REAR) and the influence of depth factors and soil type. It was hypothesized that these factors exert an effect on the physical-chemical properties of the soil, causing carbon variability. Fourteen sampling units were established for the three ecosystems; 1) Intervened, 2) Deciduous Forest and 3) Deciduous shrub forest, two soil samples were extracted from each unit at depths of (0-10 and 10-30 cm), giving a total of 28 samples. The results revealed significant differences between the three types of ecosystems, allowing us to affirm that, in the REAR, the COS content is higher in the deciduous forest ecosystem than in the deciduous shrub forest and the Intermediate zone. Likewise, Alfisol and Entisol soils in the deciduous forest had the highest COS storage capacity, being the surface layer the one with the highest accumulation in the three ecosystems. It was concluded that low precipitation, increased temperature and sporadic vegetation produced by anthropic activities affect COS storage, limiting the provision of ecosystem services related to soil carbon sequestration.

Abstract Introduction: Evidence suggests that herbivores, such as peccaries, shape vegetation structure and diversity through predation, trampling, dispersal, and rooting behavior. Objective: To evaluate the impact of peccaries (Dycotiles tajacu) on the understory vegetation of the tropical rainforest in the Nogal-La Selva Local Biological Corridor, Costa Rica, comparing a site with the absence of peccaries to another with the presence of these animals. Methodology: From June to November 2021, 20 experimental exclusions and 20 free access plots, each measuring 2 m2 were used to quantify herbivory, the number of leaf blades, damaged leaves, healthy leaves, sapling height, and fallen biomass at both sites. Results: A higher sapling density was found in the Nogal Reserve, but a lower sapling diversity, while in La Selva there was a higher sapling diversity, but a lower density of seedlings. Herbivory and sapling height in La Selva exceeded those in Nogal. The exclusion of peccaries reduced seedling damage but did not affect the dynamics of fallen biomass. Conclusion: For the design, implementation, and evaluation of the effectiveness of biological corridors, it is crucial to consider plant-animal interactions to enhance the flow of ecological processes through functional and structural connectivity, analyzed from interactions such as those presented in this paper.

Resumen Introducción: Existe evidencia que herbívoros, como los saínos, dan forma a la estructura y diversidad de la vegetación a través del comportamiento de depredación, pisoteo, dispersión y enraizamiento. Objetivo: Evaluar el impacto de los saínos (Dycotiles tajacu) en la vegetación del sotobosque del bosque tropical húmedo en el Corredor Biológico Local Nogal-La Selva, Costa Rica, en un sitio con ausencia y en otro con presencia de saínos. Métodos: De junio a noviembre de 2021 se utilizaron 20 exclusiones experimentales y 20 parcelas de acceso libre de 2 m2, se cuantifico la herbivoría, número de láminas foliares, hojas dañadas, hojas sanas, altura de brinzales y biomasa caída en ambos sitios. Resultados: Se encontró una mayor densidad de brinzales en Reserva Nogal pero una menor diversidad, contrario en La Selva donde se encontró una mayor diversidad de brinzales, pero una menor densidad de plántulas. La herbivoría y la altura de brinzales en La Selva fue mayor que en Nogal. La exclusión de los saínos disminuyó el daño a las plántulas, pero no afectó la dinámica de la biomasa caída. Conclusión: Es necesario contemplar para el diseño, implementación y evaluación de la efectividad de corredores biológicos, las interacciones planta-animal, para potencializar el flujo de procesos ecológicos mediante la conectividad funcional y estructural, analizada a partir de interacciones como las presentadas en este trabajo.

Abstract Introduction: Bioindicator monitoring is a crucial conservation strategy within natural protected areas. Various taxonomic groups have been investigated as bioindicators due to their distinctive attributes, including ease of sampling, differential responses to disturbances, associations with species richness, and accessible taxonomy. Objective: To compare the potential of four taxa (Chilopoda, Scarabaeidae, Carabidae, and Pompilidae) as environmental bioindicators, considering their ease of collection, presence of changes in community composition due to disturbances, including species with preferences for disturbed or conserved areas; and to correlate their diversity with the diversity of other taxa. Methods: The study was conducted at the Manu Learning Centre Biological Station in the Manu Biosphere Reserve, which comprises three areas along a disturbance gradient. Specimens were collected using pitfall traps, yellow pan traps, Malaise traps, and monoliths from May to June in 2021. Ease of collection was measured in terms of richness and abundance; changes in the community composition were assessed using similarity analysis, non-metric multidimensional scaling, and beta diversity partitioning; presence of species with preferences was analyzed using correspondence analysis and G. Williams’ goodness-of-fir test; and diversity correlation using the Spearman index. Results: Among the taxa investigated, Scarabaeidae and Pompilidae were the easiest to collect. Changes in composition were observed in all taxa but were significant only in Chilopoda and Scarabaeidae. All taxa exhibited species with preferences for disturbed and undisturbed environments. There was a high correlation in diversities among Carabidae, Scarabaeidae, and Chilopoda. Conclusions: Our findings underscore the diverse responses exhibited by taxa along a disturbance gradient. Notably, Scarabaeidae and Chilopoda emerged as particularly robust bioindicators within the studied ecosystem.

Resumen Introducción: El monitoreo de bioindicadores es una herramienta importante para la conservación de áreas naturales protegidas. Diferentes grupos taxonómicos fueron estudiados como bioindicadores por presentar: facilidad de muestreo, respuestas variadas a la perturbación, riqueza asociada a otras especies, y taxonomía accesible. Objetivo: Comparar el potencial de cuatro taxones (Chilopoda, Scarabaeidae, Carabidae y Pompilidae) como bioindicadores ambientales, tomando en cuenta su facilidad de recolección, presencia de cambios en la composición de sus comunidades por perturbaciones, presencia de especies con preferencia por áreas perturbadas o conservadas; y diversidad correlacionada con la diversidad de los otros taxones. Métodos: El trabajo se realizó en la estación Biológica Manu Learning Centre en la Reserva de Biosfera del Manu, que comprende tres áreas en un gradiente de perturbación. Los ejemplares fueron recolectados con trampas pitfall, bandejas amarillas, trampas Malaise y monolitos, de mayo a diciembre del 2021. La facilidad de recolección se midió en términos de riqueza y abundancia; los cambios en la composición de sus comunidades mediante el análisis de similitud, escalamiento no métrico multidimensional, y partición de la diversidad beta; presencia de especies con preferencia con el análisis de correspondencia y el ajuste de bondad G de Williams; y finalmente la correlación de las diversidades con el índice de Spearman. Resultados: De los taxones estudiados, las de más fácil recolección fueron Scarabaeidae y Pompilidae. Existen cambios en la composición en todos los taxones, pero son importantes solo en Chilopoda y Scarabaeidae. En todos los taxones hay especies con preferencia por ambientes perturbados y no perturbados. Existe una alta correlación de las diversidades de Carabidae, Scarabaeidae y Chilopoda. Conclusiones: Todos los grupos responden de diferente forma a los gradientes de perturbación, Scarabaeidae y Chilopoda fueron los que tuvieron mejores características para ser considerados buenos bioindicadores.

Abstract Introduction: Amazonian forests with varying degrees of anthropogenic disturbance in the process of natural regeneration harbour many sympatric primate species. However, studies on their usage by primates are still lacking. Objective: To comprehensively study the spatial and temporal dynamics within the primate community in a regenerating forest within the Manu Biosphere Reserve. Methods: Two sets of historical data from Crees Foundation for Manu were analyzed, comprising records from January 2011 to February 2023 and corresponding to data obtained through incidental data and Terrestrial In-line Transects in three types of forest with different degrees of historical disturbance. Results: Lagothrix lagothricha and Ateles chamek showed a preference for less disturbed forests; Plecturocebus toppini, for more disturbed forests; and the other species did not show a clear preference. Regarding vertical stratification, A. chamek, L. lagothricha and Alouatta sara showed a preference for the upper stratum, P. toppini, Saimiri boliviensis and Sapajus macrocephalus showed a preference for the middle and upper strata and Leontocebus weddelli and Aotus nigriceps, for the middle stratum. According to the temporal activity of each species, seven species (A. chamek, S. macrocephalus, S. boliviensis, P. toppini, L. lagothricha, L. weddelli, and A. sara) were observed during the day, four were similar. Conclusions: The primate community varies according to disturbance gradient due to the preferences of some species. The recorded primates make a differentiated use of the vertical strata and most of them show diurnal activity.

Resumen Introducción: Los bosques amazónicos con diferentes grados de perturbación antrópica en proceso de regeneración natural albergan gran cantidad de especies de primates en simpatría. Sin embargo, aún es carente el estudio de su uso por primates. Objetivos: Estudiar la dinámica espacial y temporal dentro de la comunidad de primates en un bosque en regeneración en la Reserva de Biosfera del Manu. Métodos: Se analizaron dos sets de datos históricos de Crees Foundation for Manu que comprenden registros desde enero 2011 hasta febrero 2023 y corresponden a datos incidentales y transectos lineales terrestres en tres tipos de bosque con diferente grado de perturbación histórica. Resultados: Lagothrix lagothricha y Ateles chamek mostraron preferencia por bosques menos perturbados; Plecturocebus toppini, por bosques más perturbados; y las demás especies no mostraron una preferencia clara. En cuanto a la estratificación vertical, A. chamek, L. lagothricha y Alouatta sara mostraron preferencia por el estrato alto; P. toppini, Saimiri boliviensis y Sapajus macrocephalus mostraron preferencia por los estratos medio y alto, y Leontocebus weddelli y Aotus nigriceps, por el estrato medio. De acuerdo con la frecuencia horaria de cada especie se observó que siete especies (A. chamek, S. macrocephalus, S. boliviensis, P. toppini, L. lagothricha, Leontocebus weddelli y A. sara) fueron observadas durante el día, cuatro fueron similares. Conclusiones: En el estudio, la comunidad de primates cambió en el gradiente de perturbación debido a las preferencias de algunas especies. Los primates registrados hacen un uso diferenciado de los estratos verticales y la mayoría de ellos mostraron períodos de actividad diurnos.

Abstract Introduction: Urban development and expansion have led to the loss and transformation of Andean ecosystems. However, remnant forests that maintain biodiversity still exist around Colombia’s major cities, but these forests are threatened by the road infrastructure. Road ecology in the Neotropics has been focused specifically in documenting the effects of roadkill from a taxonomic point of view, mainly in rural areas. Objective: To evaluate ecologically and spatially, the collision of fauna on roads located in areas of urban expansion in the Northern Colombian Andes. Methods: We assessed vertebrate mortality rate and identified the critical areas of roadkill by taxonomic and functional groups. This was achieved through spatial ecology analysis, specifically Kernel density analysis and hotspot spatial statistical analysis. Additionally, we identified landscape features associated with road mortality and assessed the role of roads as resistance and permeability factors. Results: A total of 279 roadkilled animals belonging to 64 species were found, accounting for 28 % of the 273 vertebrate species recorded in the reserve near the studied roads. The average mortality rate for vertebrates was estimated from 42 to 170 individuals annually. Differential patterns were detected in roadkill density across taxonomic and functional groups, where nocturnal species and the trophic guilds are the most vulnerable. Likewise, it was found that the sections of the roads close to the forest have a greater roadkill species richness with greater ecological requirements. These sections constitute an important resistance to species movement in the area. Conclusions: Road mortality in the study area exhibits heterogeneous spatial patterns not only at a taxonomic but also at a functional level, suggesting the deterioration of protected ecosystems in adjacent peri-urban areas.

Resumen Introducción: El desarrollo y expansión urbana han generado la pérdida y transformación de los ecosistemas andinos. Sin embargo, alrededor de las grandes ciudades colombianas aún se encuentran relictos boscosos que mantienen gran riqueza de biodiversidad, la cual es amenazada por la infraestructura vial. El énfasis de los trabajos de Ecología de Carreteras en el Neotrópico ha estado centrado en el efecto del atropellamiento de fauna desde el punto de vista taxonómico. Objetivo: Evaluar, ecológica y espacialmente, el atropellamiento de la fauna en carreteras ubicadas en zonas de interés para la expansión urbana del norte de los Andes colombianos. Métodos: Se evaluaron la tasa de mortalidad de vertebrados, las zonas críticas de atropellamientos por grupo taxonómico y grupos funcionales. Esto se realizó por medio de análisis de ecología espacial, específicamente análisis de densidad de Kernel y análisis de estadística espacial de puntos calientes. Adicionalmente, se identificó las características del paisaje asociadas al atropellamiento y se evaluó el papel de las vías como factor de resistencia y permeabilidad. Resultados: Se encontraron 279 animales atropellados de 64 especies que representan el 28 % de las 273 especies vertebradas presentes en las áreas protegidas en inmediaciones de las carreteras estudiadas. Se estimó una tasa promedio entre 42 y 170 individuos atropellados por año. Se detectaron patrones diferenciales en la densidad de atropellamiento por grupos taxonómicos y funcionales, donde las especies nocturnas y el tipo de gremio trófico son los más vulnerables. Asimismo, se encontró que los tramos de las vías en cercanía a coberturas boscosas presentan mayor riqueza de especies atropelladas y con mayores requerimientos ecológicos. Estos tramos representan una resistencia importante al movimiento de las especies de la zona. Conclusiones: El atropellamiento presenta patrones espaciales heterogéneos, no solo a nivel taxonómico, sino también a nivel funcional, lo cual representa un deterioro para los ecosistemas protegidos en zonas periurbanas.

Abstract Background: As a result of botanical explorations carried out for the project Bromeliaceae of Mexico, we collected plants of the genus Hechtia within the Sierra de Huautla Biosphere Reserve, in Xochipala, municipality of Tlaquiltenango, Morelos, Mexico. However, this plant does not resemble any known species. Question: Do the Hechtia populations in Xochipala correspond to an undescribed taxon? Studied species: Species of Hechtia previously reported from Morelos and all the taxa with greater morphological similarity to the new proposed taxon. Study site and date: Xochipala, municipality of Tlaquiltenango, Morelos, Mexico, 2023. Methods: Our research involved field work, review of herbarium specimens, protologues, and type material of all the species of Hechtia distributed in Morelos, in the neighboring states of Puebla and Guerrero, and of the most morphologically similar taxa. Results: After a careful and detailed review of the living material and herbarium specimens, we conclude that those plants belong to an undescribed taxon that we propose here. A morphological description, images, and a distribution map of Hechtia cerrostlatilpae are included, as well as an identification key and a list of examined specimens of all Hechtia species known from the state of Morelos. Conclusions: The discovery of a new species of Hechtia in Xochipala, Morelos, Mexico increases the plant diversity of Morelos, particularly at the Sierra de Huautla Biosphere Reserve. Hechtia cerrostlatilpae presents unique vegetative and floral characters that allow its recognition as a new species within the genus.

Resumen Antecedentes: Como resultado de exploraciones botánicas realizadas para el proyecto Bromeliaceae de México, se recolectaron plantas del género Hechtia dentro de los límites de la reserva de biosfera Sierra de Huautla en Xochipala, municipio de Tlaquiltenango, Morelos, México. Sin embargo, esta planta no se parece a ninguna especie conocida. Pregunta: ¿Las poblaciones de Hechtia en Xochipala corresponden a un taxón no descrito? Especies de estudio: Especies de Hechtia reportadas para Morelos y todos los taxa morfológicamente más similares al nuevo taxón propuesto. Sitio y año de estudio: Xochipala, municipio de Tlaquiltenango, Morelos, México, 2023. Métodos: Nuestra investigación implicó trabajo de campo, revisión de especímenes de herbario, protólogos y material tipo de todas las especies de Hechtia distribuidas en Morelos, en los estados vecinos de Puebla y Guerrero y también, de los taxones más similares morfológicamente. Resultados: Tras una cuidadosa y detallada revisión de material vivo y de ejemplares de herbario, concluimos que estas plantas son un taxón no descrito que aquí proponemos. Se incluye una descripción morfológica, imágenes y un mapa de distribución de Hechtia cerrostlatilpae, así como una clave de identificación y una lista de especímenes examinados de todas las especies de Hechtia conocidas en el estado de Morelos. Conclusiones: El descubrimiento de una nueva especie de Hechtia en Xochipala, Morelos, México, aumenta la diversidad de plantas en Morelos, particularmente en la reserva de la biosfera Sierra de Huautla. Hechtia cerrostlatilpae presenta caracteres vegetativos y florales únicos que permiten su reconocimiento como una nueva especie del género.

Remote areas that lack conventional water-provisioning infrastructure often rely on rainwater harvesting, rivers, pans, reservoirs and borehole-extracted water to meet domestic water requirements. These water sources often have poor microbial quality and chemical composition, the quality of which is not routinely monitored. This study explored citizen science as a tool for Engaged Research and Responsible Research and Innovation, detailing the co-creation of a sustained community-based water quality monitoring program in collaboration with communities in villages in Amakhala Game Reserve (Eastern Cape, South Africa). Without access to other water sources, participants predominantly used rainwater for drinking and cooking (80%), while borehole water was mainly used for cleaning and gardening due to its salty or bitter taste. A hydrogen sulfide (H2S) water testing kit was used by the citizen scientists to monitor the water quality. The H2S kits were effective in estimating bacterial contamination, showing a proportional relationship with Colilert® test results conducted in a laboratory. The alignment observed between community-based monitoring results and those derived from scientist-led testing underscores the value of data produced through citizen science initiatives. Sustained participant engagement throughout this research reflected a sense of community empowerment through access to tools that inform their decision-making around water use and treatment as well as investment in the research, indicative of the perceived relevance of the research to community interests. This integration of transdisciplinary data sources holds promise for informing evidence-based decision-making processes, facilitating more effective and contextually informed water management strategies that value and integrate community perspectives alongside scientific insights. SIGNIFICANCE: Drawing on the principles of Engaged Research and Responsible Research and Innovation, we applied citizen science tools to engage researchers and communities in the co-development of a water quality monitoring program. In addressing a specific area of concern, raised by the remotely situated community around the need for knowing their water quality, the project successfully trained communities in applying water testing tools and fostered agency in decision-making around water treatment. Combined with continuous feedback and communication loops, a high rate of continuity was sustained among the participants.

Antibiotic resistance is a global threat, with a disproportionate burden of mortality in low- and middle-income countries. It is increasing in both the public and private healthcare sectors within South Africa, especially in Gram-negative organisms, and is associated with increased use of World Health Organization watch and reserve antibiotics. There is a need for improved access to new antibiotics to treat infections caused by drug-resistant organisms in order to limit side-effects and improve patient outcomes of currently available antibiotics. We propose the responsible introduction of these new antibiotics with both administrative and clinical oversight in order to preserve the longevity of these precious antibiotics.

Die inflasiekoers en die produksiegaping speel 'n belangrike rol in die monetêrebeleidstand-punt. Alhoewel die publiek inflasie redelik goed verstaan, is die produksiegaping 'n relatief onbekende begrip. Definisies van potensiële produksie kan dubbelsinnig wees, aangesien die term dikwels gebruik word om verwante, maar logies afsonderlike, konsepte te beskryf. Die produksiegaping word ook nie direk waargeneem nie en word afgelei uit beramings van potensiële produksie, wat self nie waarneembaar of meetbaar is nie en dus beraam moet word. Hierdie beramings kan egter sensitief wees vir verskillende metodologiese paradigmas en databeskikbaarheid. Die doel van hierdie artikel is om by te dra tot 'n beter begrip van en groter deursigtigheid in plaaslike monetêre beleid deur die rol van die produksiegaping in Suid-Afrikaanse monetêre beleid te verduidelik. Eerstens word die idees onderliggend aan die konsep van die produksiegaping bespreek, waarna 'n werksdefinisie van die produksiegaping in die konteks van monetêre beleid voorgestel word. Verskeie metodologieë en empiriese tegnieke waarvolgens potensiële produksie en die produksiegaping beraam kan word, word bespreek, insluitende eenveranderlike statistiese tegnieke, strukturele modelle en semistrukturele benaderings. Die artikel verduidelik voorts hoe die Suid-Afrikaanse Reserwebank (SARB) die plaaslike produksiegaping beraam en hoe hierdie beraamde produksiegaping op die monetêrebeleidstand-punt inwerk. Ten slotte word die SARB se monetêrebeleidstandpunt beskou met betrekking tot die inflasiekoers en produksiegapingsberamings oor die afgelope twee dekades ten einde die reaksie van die plaaslike monetêre beleid op groot ekonomiese skokke soos die wêreldwye finansiële krisis en COVID-19 te illustreer, en ten einde die beramings se sensitiwiteit vir datahersienings uit te lig.

The inflation rate and the output gap are two of the main determinants of the monetary policy stance. However, while inflation is relatively well understood by the public, the output gap is not. The output gap is not directly observed and is derived from estimates of potential output. Potential output is also not observable or measurable, and therefore needs to be estimated. Such estimates can, however, be sensitive to different methodological paradigms and the availability of data, while definitions of potential output may be ambiguous, because the term is often used to describe related, but logically distinct, concepts. With the aim of contributing to a better understanding of and greater transparency in domestic monetary policy, this paper describes the role of the output gap in South African monetary policy by broadly examining the following three questions: (i) What exactly is the output gap?; (ii) How is it measured? and (iii) Why is it important? To begin with, the idea underlying the concept of the output gap is explained and a tentative working definition of the output gap in the context of monetary policy is provided. Various methodologies and empirical techniques of estimating potential output and the output gap are then discussed, including univariate statistical techniques, structural models and semi-structural approaches. This is followed by an explanation of how the South African Reserve Bank (SARB) estimates the domestic output gap, and how the estimated output gap feeds into the monetary policy stance. Finally, the SARB's monetary policy stance is viewed in relation to the inflation rate and output gap estimates over the last two decades to illustrate the domestic monetary policy response to notable economic events such as the global financial crisis and the COVID-19 shock, and to highlight the sensitivity of the estimates to data revision. This paper shows that both actual and potential economic growth have declined over the last decade, even without taking into account the COVID-19 shock. This is consistent with the South African empirical literature, and points to a worrying and persistent stagnation in domestic economic activity. After strong economic activity during the early 2000s, the economy started slowing down in 2008. This may be ascribed to a number of factors, including the introduction of load-shedding in 2007, changes in the global economic landscape following the globalfinancial crisis, dwindling infrastructure investment after the 2010 FIFA World Cup tournament, and uncertainty about domestic economic policy. COVID-19 brought about a massive economic shock in 2020. Lockdown regulations saw capital and labour withdrawn from economic use, which caused real GDP and potential output to contract by 6,2% and 2,7%, respectively, for the year. The output gap, which has mainly remained in negative territory since 2009, fell to -7,9% in the second quarter of 2020. In response to the crisis, the SARB drastically lowered the policy rate and applied several other financial market interventions, but even this extensive range of stimuli failed to prevent an economic collapse. Moreover, the output gap has remained negative despite a minor rebound of the economy in 2021 and 2022, which indicated that economic activity remained on its weakpre-COVID-19 trajectory. Inflation has stabilised markedly since 2010. This may be partly due to weak domestic economic activity that does not exert significant domestic inflationary pressures. In fact, many of the notable inflationary episodes of the last 25years were largely caused by external factors, such as the rand depreciation shock and the global financial crisis. This benign domestic inflationary environment has arguably made it easier for the SARB to achieve its inflation target. Such consistency has contributed to an increase in the SARB's credibility, which, in turn, has led to inflation expectations that are lower and better anchored.

RESUMEN Introducción: El precursor del factor de crecimiento epidérmico está inmerso en el contexto de la membrana plaquetaria. El procedimiento actual para obtener suero implica un reto para una interpretación suficiente del significado biológico de sus valores. Objetivo: Describir el efecto de la modificación del procedimiento de obtención de muestra para la determinación del factor de crecimiento epidérmico, y las posibles implicaciones biológicas de sus resultados. Método: Se llevó a cabo un estudio experimental in vitro, con muestras de sangre de sujetos con antecedentes de salud aparente, en edad adulta. Se determinaron los valores de factor de crecimiento epidérmico sérico con el kit de UMELISA-EGF según recomendaciones del fabricante; la variación experimental en la obtención de la muestra se realizó según recomendaciones de SAMERSAC para plasma rico en plaquetas, con ajustes. Resultados: En la casuística, la edad promedio fue de 45.89± 3.35 años, con rango de 27-62 años. Predominó el sexo femenino en una razón 1.8:1. La aplicación del procedimiento experimental evidenció diferencias significativas y notorias entre los valores de EGF (g=17.7; p=0.0001-23); no se evidenció correlación lineal entre los valores (r= 0.1305; p= 0.6843). El valor de “reserva”, fue en promedio de 1498.61±160.68 pg*mL-1, observándose diferencias de efecto grande, así como un comportamiento similar a un valor de transición. Los datos observados sugieren, a posteriori, un efecto de consumo en el curso de la lisis plaquetaria en condiciones estándar. Conclusiones: Los valores del factor de crecimiento epidérmico son superiores luego del aislamiento de las plaquetas; la existencia de una reserva intraplaquetaria de factor de crecimiento epidérmico es plausible.

ABSTRACT Introduction: Epidermal growth factor precursor is immersed in the context to Platelets membrane. The regular procedure to obtain serum implies a barrier to biological understanding of the theoretical meaning of its values. Objective: To describe the effect to modified procedure to measure sample epidermal growth factors and the possible biological implications for this. Method: An in vitro experimental study was carried put, with blood samples from individuals with apparent health history, in adulthood. Serum epidermal growth factor precursor values were determined with the UMELISA-EGF kit recommended by the manufacturer; the experimental variation in sample collection was carried out according to SAMERSAC recommendations for platelet-rich plasma, with adjustments. Results: The average age was 45.89± 3.35 years, in a range of 27 to 62 years. The female sex predominates in a 1.8:1 ratio. Application of the experimental procedure showed significant and notable differences between the epidermal growth factor values (g=17.7; p=0.0001-23); no linear correlation was evident between the values (r= 0.1305; p= 0.6843). The "reserve" value was on average 1498.61 ±160.68 pg*mL-1, observing large effect differences, as well as a similar behavior to a transition value. The observed data suggest, a posteriori, consumption effect on the course of platelet lysis under standard conditions. Conclusions: Epidermal growth factor values are higher after platelet isolation; the existence of an intraplatelet reserve of epidermal growth factor is plausible.

RESUMO As Unidades de Conservação (UC) constituem domínios territoriais estratégicos para a gestão socioambiental na Amazônia. Neste estudo, questiona-se como os moradores e gestores de uma UC definem seus territórios de sustentabilidade para a Amazônia, especificamente, em atenção à saúde. Para isso, foi realizada pesquisa de campo em uma comunidade localizada na Reserva de Desenvolvimento Sustentável do Rio Negro. Para a coleta de dados, foram utilizadas técnicas, como entrevistas, diário de campo e observação direta. Como resultados, destaca-se que grande parte das famílias possuía sustento baseado em uma unidade de produção familiar, cuja renda média mensal é abaixo de um salário mínimo, e gerada principalmente na agricultura e pesca. Dadas as condições de vida e saúde na reserva, gestores e moradores entrevistados problematizam os pressupostos sustentáveis que definem os atuais modelos de UC na região amazônica, visto que não apresentam ações estratégicas para o efetivo desenvolvimento humano e social de suas famílias. A noção de saúde na Amazônia está imbricada no controle coletivo sobre esses territórios, e se ela não é suficientemente ampla para compreendê-los, precisa ser reelaborada. (UC estudo questionase questiona especificamente isso Negro dados técnicas entrevistas direta resultados destacase destaca familiar mínimo pesca reserva amazônica compreendêlos, compreendêlos compreendê los, los compreendê-los reelaborada

ABSTRACT Conservation Units (UCs) constitute strategic territorial domains for social and environmental management in the Amazônia. In this study, we question how residents and managers of a UC define their sustainability territories, specifically, in health care. Field research was carried out in a community located in the Rio Negro Sustainable Development Reserve. For data collection, techniques such as interviews, field diary and direct observation were used. As a result, their empirical data show that a large proportion of families had a livelihood based on a family production unit, with an average monthly income below the minimum wage, and generated mainly in agriculture and fishing. Considering the living and health conditions in the reserve, managers and residents interviewed problematize the sustainable assumptions that define current models of UC in the Amazônia, since they do not have strategic actions for the effective human and social development of their families. The notion of health in the Amazon is embedded in collective control over these territories, and if it is not broad enough to encompass them, it needs to be redefined. UCs (UCs Amazônia study territories specifically care Reserve collection interviews used result unit wage fishing reserve them redefined

Abstract The natural durability of wood is defined as its intrinsic ability to resist degradation caused by biotic and abiotic factors. There are few records of natural wood durability in Mexico; in this study, it was determined based on the degradation of the wood of six commercial species: Pinus durangensis, P. strobiformis, P. cooperi, Juniperus deppeana, Quercus sideroxyla, and Alnus acuminata, in addition to Fagus sylvatica as the control. Test specimens were placed in contact with soil for 12 months at the Mineral de Nuestra Señora de la Candelaria Ecological Reserve of in Cósala, Sinaloa, and at the Las Bayas property in the state of Durango. Five specimens of each species were buried at 50 % of their length and analyzed following the UNE-EN 252:2015 European standard for determining degradation in wood, and UNE-EN 350:2016 for estimating durability. Due to the absence of normality (p<0.05) in the degradation data, the Kruskall-Wallis nonparametric test was used to determine statistical differences between species, locality, and puncture area. Statistical analysis was performed in the R-Studio program. According to UNE-EN 252:2015 and UNE-EN 350:2016, J. deppeana was the most durable wood classified as Class 1; Q. sideroxyla, P. strobiformis, P. durangensis and F. sylvatica (control) were Class 2 timbers, with slight damage; and A. acuminata and P. cooperi were classified as Class 3, with evident degradation. It is concluded that J. deppeana has the most durable wood.

Resumen La durabilidad natural de la madera se define como la capacidad intrínseca que tiene para resistir la degradación ocasionada por factores bióticos y abióticos. Existen pocos registros de la durabilidad natural de la madera en México; en este estudio se determinó con base en la degradación de la madera de seis especies comerciales: Pinus durangensis, P. strobiformis, P. cooperi, Juniperus deppeana, Quercus sideroxyla y Alnus acuminata, además de Fagus sylvatica como testigo. Se usaron probetas colocadas en contacto con el suelo por 12 meses en dos localidades: la Reserva Ecológica del Mineral de Nuestra Señora de la Candelaria en Cósala, Sinaloa, y el predio Las Bayas, Durango. Cinco probetas de cada especie se enterraron a 50 % de su longitud y se analizaron siguiendo la norma europea UNE-EN 252:2015 que determina la degradación en la madera, y la UNE-EN 350:2016 para la durabilidad. Debido a la ausencia de normalidad (p<0.05) en los datos de degradación, se usó la prueba no paramétrica de Kruskall-Wallis para determinar diferencias estadísticas entre especies, localidad y área de punzado. El análisis estadístico se realizó en el programa R-Studio. De acuerdo con las normas UNE-EN 252:2015 y UNE-EN 350:2016, J. deppeana fue la madera más durable clasificada como Clase 1; Q. sideroxyla, P. strobiformis, P. durangensis y F. sylvatica (control), fueron maderas Clase 2, con ligero daño; A. acuminata y P. cooperi clasificaron como Clase 3, con evidente degradación. Se concluye que J. deppeana presenta la madera más durable.