Abstract: The aim of the article is to evaluate and understand the feelings and attitudes of Brazilian physicians regarding the implementation of palliative care in pediatric patients. Between July 2018 and December 2019, 236 questionnaires were sent to physicians on the National Academy of Palliative Care roster. The data collected through the planned questionnaires were analyzed using the IRAMUTEQ programs (Interface de R pour les Analyzes Multidimensionnelles de Textes et de Questionnaires). From the analysis performed by IRAMUTEQ, a dendrogram containing words with p<0.05 was obtained. Feelings of sadness, anguish, relief, regret and fulfillment were perceived as relevant. Attitudes such as understanding, communication, instructing, treating well, strategy, and multiprofessional conferences were also described. Thus, it was observed that these professionals experience different contradictory feelings and make use of different strategies so that they do not harm the treatment carried out, in addition to maintaining a good relationship with the patient’s family. In the present study, we demonstrate that there are intrinsic characteristics of pediatric palliative care that are a source of mixed feelings for medical professionals. In this context, challenges still exist and it is essential to conduct new studies that address this issue. Paying attention to these feelings promotes better management of palliative care in pediatrics.

Resumen: El objetivo del artículo es evaluar y comprender los sentimientos y actitudes de los médicos brasileños en relación con la implementación de los cuidados paliativos en pacientes pediátricos. Entre julio de 2018 y diciembre de 2019, se enviaron 236 cuestionarios a los médicos de la lista de la Academia Nacional de Cuidados Paliativos. Los datos recogidos a través de los cuestionarios previstos se analizaron utilizando los programas IRAMUTEQ (Interface de R pour les Analyzes Multidimensionnelles de Textes et de Questionnaires). A partir del análisis realizado por el IRAMUTEQ, se obtuvo un dendrograma que contenía palabras con p<0,05. Sentimientos de tristeza, angustia, alivio, arrepentimiento y realización fueron percibidos como relevantes. También se describieron actitudes como comprensión, comunicación, instruir, tratar bien, estrategia y conferencias multiprofesionales. Así, se observó que estos profesionales experimentan diferentes sentimientos contradictorios y hacen uso de diferentes estrategias para que no perjudiquen el tratamiento realizado, además de mantener una buena relación con la familia del paciente. Demostramos que existen características intrínsecas de los cuidados paliativos pediátricos que son fuente de sentimientos encontrados para los profesionales médicos. En este contexto, aún existen desafíos y es esencial realizar nuevos estudios que aborden este tema. La atención a estos sentimientos promueve una mejor gestión de los cuidados paliativos en pediatría.

Resumo: O objetivo desse artigo é avaliar e compreender os sentimentos e atitudes de médicos brasileiros no que diz respeito à implementação de cuidados paliativas em pacientes pediátricos. Entre julho de 2018 e dezembro de 2019, foram enviados 236 questionários a médicos do rol da Academia Nacional de Cuidados Paliativos. Os dados coletados através dos questionários planejados foram analisados usando os programas IRAMUTEQ (Interface de R pour les Analyzes Multidimensionnelles de Textes et de Questionnaires). Da análise realizada pelo IRAMUTEQ, obteve-se um dendrograma contendo palavras com p<0.05. Sentimentos de tristeza, angústia, alívio, arrependimento e satisfação foram percebidos como relevantes. Atitudes como compreender, comunicar, educar, tratar bem, estratégia e conferências multiprofissionais também foram descritas. Assim, observou-se que esses profissionais experimentam sentimentos contraditórios diferentes e fazem uso de diferentes estratégias de forma a não prejudicar o tratamento realizado, além de manter uma boa relação com a família do paciente. No presente estudo, demonstramos que há características intrínsecas de cuidados paliativos pediátricos que são fonte de sentimentos contraditórios para profissionais médicos. Nesse contexto, ainda existem desafios e é essencial conduzir novos estudos que abordem essa questão. Prestar atenção a esses sentimentos promove melhor manejo de cuidados paliativos em pediatria.

Resumo:O manuscrito apresenta os fundamentos teórico-conceituais da transdisciplinaridade e da ecoformação, aplicandoos no ensino superior através de um estudo sobre sua implementação em uma universidade particular da Costa Rica. Ainda que as reflexões se refiram à educação universitária, elas também podem ser aplicadas em outros níveis ou contextos educacionais. A relevância deste estudo é destacada ao fazer referência à crise planetária atual e à necessidade resultante de reformular o ensino universitário para enfrentar esses desafios. Em seguida, explicações básicas sobre a transdisciplinaridade são fornecidas, entendendo-a como uma nova forma de abordagem da realidade, o que suscita transformações no mundo acadêmico. Seguidamente, abordase a ecoformação, concebida como um estilo de vida baseado nas relações equilibradas entre os seres, uma vez que tudo está interligado; apostando então na convivência. Para a educação, os princípios ético-filosóficos apresentados podem ser traduzidos em conceitos pedagógicos e didáticos específicos. Por último, conclui-se que a transdisciplinaridade e a ecoformação são perspectivas para a humanidade; exigem uma mudança nas formas tradicionais de compreender a realidade e, portanto, de exercer a educação.

Resumen:El ensayo presenta fundamentos teórico-conceptuales de la transdisciplinariedad y la ecoformación, aplicándolas en educación superior; corresponde a la primera parte, la fundamentación ético-filosófica, de un estudio acerca de la puesta en práctica de estas en una universidad privada en Costa Rica. Si bien las reflexiones aluden a la educación universitaria, pueden aplicarse también en otros niveles o contextos educativos. Se introduce la importancia de este trabajo aludiendo a la crisis planetaria de la contemporaneidad y a la consecuente necesidad de cambiar la educación universitaria para responder a ella. Luego, se brindan explicaciones básicas en cuanto a la transdisciplinariedad, entendiéndola como una nueva forma de aproximarse a la realidad, lo cual implica transformaciones para la academia. De seguido, se aborda la ecoformación, concebida como un estilo de vida basado en relaciones equilibradas entre los seres, pues todo está interconectado; apuesta, entonces, por la coexistencia. Para la educación, los principios ético-filosóficos que se presentan pueden traducirse en concepciones pedagógicas y didácticas específicas. Por último, se concluye que la transdisciplinariedad y la ecoformación son perspectivas para la humanidad; requieren cambiar las formas tradicionales de entender la realidad y, por tanto, de ejercer la educación

Abstract: The manuscript presents the theoretical and conceptual foundations of transdisciplinarity and eco- training and applies them to higher education through a study of their implementation in a private university in Costa Rica. Although the reflections refer to university education, they can also be applied to other educational levels or contexts. The importance of this work is highlighted by addressing the current planetary crisis and the consequent need to adapt university education to respond to it. It then provides a basic explanation of transdisciplinarity, understood as a new way of approaching reality that implies transformations for the academy. Then, the ecoformation is approached, understood as a way of life based on a balanced relationship between beings, given that everything is interconnected, and therefore committed to coexistence. For education, the ethical-philosophical principles presented can be translated into specific pedagogical and didactic concepts. Finally, it is concluded that transdisciplinarity and ecoformation are perspectives for humanity; they require a change in the traditional ways of understanding reality and, therefore, of practicing education.

ABSTRACT The limitations and proposals to improve the relations between the academy and decision-makers in the socioeconomic sphere are exposed, considering the key role of management cadres at all levels of the economy. Although the complexity of the economy indicates that it cannot be left alone to the economists; This should not imply extreme positions that underestimate and ignore their contribution, their different criteria and alternative solutions to problems. Approval of decisions with genuinely democratic methods must also be considered. Until now, frankly vulgar conceptions about the economy and in particular about the business system have been evidenced, which have been justified on repeated occasions, under a false defense of the ideology in the socialist transition

RESUMEN Se exponen las limitaciones y propuestas para perfeccionar las relaciones entre la academia y los decisores en la esfera socioeconómica, considerando el papel clave de los cuadros de dirección en todos los niveles de la economía. Si bien la complejidad de la economía indica que no se puede dejar solo a los economistas, ello no debe implicar posiciones extremas que subestiman e ignoran su aporte, sus diferentes criterios y alternativas de solución de los problemas. Debe considerarse también la aprobación de las decisiones con métodos auténticamente democráticos. Hasta la actualidad se han evidenciado concepciones francamente vulgares sobre la economía y en particular para el sistema empresarial, las cuales se han justificado en reiteradas ocasiones, bajo una defensa falsa de la ideología en la transición socialista

Resumen: Este artículo analiza el proceso de constitución y consolidación del campo de la ecología política en América Latina como espacio interdisciplinar en las ciencias sociales. Afirmo que la configuración del campo de estudios surge de la intersección entre dinámicas sociopolíticas y académicas en dos momentos fundamentales: en un primer momento, se decantan las principales líneas de análisis, donde las proposiciones teórico-empíricas se imbrican al activismo y compromiso político de sus principales representantes con procesos específicos; en un segundo momento, se produce la formalización académica y el ensanchamiento del campo de estudios, con nuevas preguntas que se inscriben en el contexto geopolítico global de cambio climático y transición energética, donde desatacan nuevas agendas de investigación transversales en su aporte a la discusión internacional. El artículo aporta una mirada original a los debates interdisciplinares de las ciencias sociales, así como a las discusiones en la relación academia/sociedad.

Abstract: This article analyzes the process of constitution and consolidation of the field of political ecology in Latin America as an interdisciplinary space in the social sciences. We argue that the configuration of the field of study arises from the intersection between sociopolitical and academic dynamics in two fundamental moments: the first moment, the main lines of analysis, where the theoretical-empirical propositions overlap with the activism and political commitment of their principal representatives with specific processes; a second moment, of academic formalization and broadening of the field of studies, with new questions that are part of the current global geopolitical context of climate change and energy transition, where the new cross-cutting research agendas and their contribution to the discussion stand out international. The article provides an original look at the interdisciplinary debates in the social sciences and the discussions on the relationship between academia and society.

Resumen: La presente propuesta es resultado del diálogo inter-epistémico establecido entre especialistas rituales calendáricos mayas -ajq’ijab- de Guatemala con personas de la academia occidental. En ella esbozamos las ideas que sustentan este intercambio y proponemos que los sistemas contemporáneos de conteo del tiempo, dada su estrecha relación con diversos elementos y los ciclos de la naturaleza, conforman una herencia biocultural colectiva del pueblo kaqchikel, que debe ser reconocida como tal a modo de apuntalar sus procesos de reivindicación política y cultural.

Abstract: This proposal is the result of the inter-epistemic dialogue established between Mayan calendrical ritual specialists -ajq’ijab- from Guatemala with people from the western academy. In it, we outline the ideas that support this exchange and propose that contemporary timecounting systems, given their close relationship with various elements and the cycles of nature form a collective biocultural heritage of the Kaqchikel people, which must be recognized as such to support their political and cultural vindication processes.

Resumo O artigo apresenta os resultados de um estudo sobre o caminho percorrido desde o reconhecimento das competências genéricas como entidade independente, dada a sua transversalidade a qualquer área do conhecimento, das competências específicas a desenvolver por um profissional, estabelecendo a distinção de aquelas que os empregadores preferem na seleção de pessoal, por isso tem como objetivo socializar um estudo teórico sobre as competências genéricas que o setor trabalhista prioriza nos profissionais universitários que recebe. Para isso, foram utilizados métodos teóricos de pesquisa, como revisão bibliográfica, histórico-lógico, análise-síntese e indução-dedução, além de métodos empíricos, como análise documental, em conjunto com o gerenciador de código aberto JabRef, multiplataforma que opera sob o Formato BibText. Foram utilizados como instrumentos o guia de revisão bibliográfica e o guia de análise documental. Os principais resultados indicam que o domínio de competências genéricas pelos jovens profissionais que entram no mercado de trabalho é insuficiente, especialmente em nações com menos recursos. Conclui-se que é fundamental conseguir a inter-relação entre empregadores e academia para determinar as competências genéricas exigidas por cada setor e forma de propriedade, a fim de formar os profissionais necessários para o futuro imediato.

Abstract The article presents the results of a study on the path taken from the recognition of generic competencies as an entity independent of the specific competencies to be developed by a professional, given their transversal nature to any area of knowledge, establishing the distinction of those preferred by employers in the selection of personnel, so its objective is to socialize a theoretical study on the generic competencies prioritized by the labor sector in the university professionals it receives. For this purpose, theoretical research methods such as literature review, historical-logical, analysis-synthesis and induction-deduction were used, as well as empirical methods such as documentary analysis, together with the open code manager JabRef, multiplatform that operates under the BibText format. The bibliographic review guide and the documentary analysis guide were used as instruments. The main results indicate that the mastery of generic competencies by young professionals entering the labor market is insufficient, especially in nations with fewer resources. It is concluded that it is essential to achieve the interrelation between employers and academia to determine the generic competencies required by each sector and type of property, in order to train the professionals needed in the immediate future.

Resumen El artículo presenta los resultados de un estudio acerca del camino transitado desde el reconocimiento de las competencias genéricas como una entidad independiente de las competencias específicas a desarrollar por un profesional, dado su carácter transversal a cualquier área del conocimiento, estableciendo la distinción de aquellas que prefieren los empleadores en la selección del personal, por lo que su objetivo es socializar un estudio teórico sobre las competencias genéricas que prioriza el sector laboral en los profesionales universitarios que recibe. Para ello se emplearon métodos de la investigación teórica como el de revisión bibliográfica, el histórico-lógico, el análisis-síntesis y la inducción-deducción, así como métodos empíricos como el análisis documental, unidos al gestor de código abierto JabRef, multiplataforma que opera bajo el formato BibText. Como instrumentos se emplearon la Guía de revisión bibliográfica y la Guía de análisis documental. Los principales resultados indican que es insuficiente el dominio de competencias genéricas por los jóvenes profesionales que acceden al mercado laboral, especialmente en las naciones con menos recursos. Se concluye que es imprescindible el logro de la interrelación entre los empleadores y la academia para la determinación de las competencias genéricas requeridas por cada sector y tipo de propiedad, a fin de formar los profesionales que necesita el futuro inmediato.

Abstract Piaractus mesopotamicus, is a fish usually farmed in semi-intensive systems with access to natural food and supplementary feed. This study evaluates effects of feed allowance on the productive performance, carbon turnover and proportions of nutrient (carbon) contribution of feed and natural food for the growth of pacu. Juvenile fish were stocked in fiberglass tanks and fed to 100, 75, 50, 25, 0% apparent satiety (ApS), with a practical, extruded (C4 photosynthetic pathway) feed in a randomized design trial (n=3); plankton production for simulated semi-intensive farming system condition was induced by chemical fertilization. A control treatment was set up in tanks devoid of natural food. Data on muscle stable carbon isotope ratios were used to study carbon turnover using a relative growth-based model. Low variation of the δ13C impaired fitting a turnover model curve for the 0 and 25 % ApS treatments. Fish of the 100% and 75% ApS treatments reached circa 95% and 82.85% of the carbon turnover, respectively. Extruded feed was the main nutrient source for the growth of pacu in the semi-intensive, simulated farming condition. The current study contributes to the knowledge of the relationship between feeding rates and carbon turnover rates in the pacu muscle. mesopotamicus semiintensive semi intensive performance (carbon 100 75 50 ApS, , (ApS) practical C4 C (C pathway n=3 n3 n 3 (n=3) fertilization growthbased based δC δ 2 95 8285 82 85 82.85 respectively semiintensive, intensive, 10 7 5 (ApS n= (n=3 9 828 8 82.8 1 (n= 82. (n

Abstract Platinum nanoparticles supported by carbon nanotubes were obtained by a simple chemical route and used for preparation of electrochemical sensor towards caffeine determination. Carbon nanotubes were used before and after an acid treatment, yielding two different materials. Morphological and structural characterization of these materials showed platinum nanoparticles (size around 12 nm) distributed randomly along carbon nanotubes. Modified electrodes were directly prepared through a dispersion of these materials. Voltammetric studies in the presence of caffeine revealed an electrocatalytic effect of platinum oxides, electrochemically produced from the chemical oxidation of the platinum nanoparticles. This behavior was explored in the development a selective method for caffeine determination based on platinum oxide reduction at a lower potential value (+0.45 V vs. Ag/AgCl). Using the best set of experimental conditions, it was shown a linear relationship for the caffeine concentration ranging from 5.0 to 25 µmol L-1 with a sensitivity of 449 nA L µmol-1. Limits of detection and quantification of 0.54 and 1.80 µmol L-1 were calculated, respectively. Recovery values for real samples of caffeine pharmaceutical formulations between 98.6% and 101.0% (n = 3) were obtained using the proposed procedure. Statistical calculations showed good concordance (95% confidence level) between the added and recovery values. treatment size 1 nm oxides +0.45 045 0 45 (+0.4 vs Ag/AgCl. AgAgCl Ag/AgCl . Ag AgCl Ag/AgCl) conditions 50 5 5. 2 L1 L- 44 µmol1. µmol1 1. µmol-1 054 54 0.5 180 80 1.8 calculated respectively 986 98 6 98.6 1010 101 101.0 n 3 procedure 95% 95 (95 level +0.4 04 4 (+0. µmol- 05 0. 18 8 9 98. 10 101. (9 +0. (+0 ( +0 (+ +

RESUMEN Los diferentes grupos de interés y sus aspectos de influencia con respecto a la asignación de valor han ganado una creciente demanda por parte de las organizaciones y la academia. En la literatura reciente sobre stakeholders, existe un desarrollo teórico sobre la distribución de valor considerando atributos de importancia estratégica y poder; sin embargo, la literatura aún carece de estudios empíricos para analizar y comprender la relación. Este artículo tiene como objetivo verificar la asociación entre el poder y la importancia estratégica de los stakeholders y la distribución de valor a ellos por parte de las empresas que cotizan en la Bolsa de Valores de Toronto (TSX). Para obtener la información relevante sobre el trato que las empresas dan a sus grupos de interés, analizamos el contenido de 104 prospectos del proceso de OPI de la TSX de 2008 a 2019. Los resultados revelaron que el poder y la importancia estratégica son relevantes para la distribución de valor a los grupos de interés y que la importancia estratégica de los stakeholders tiene una influencia más significativa en comparación con su poder. En cuanto a las contribuciones, nuestro estudio enriquece los debates previos en la literatura sobre stakeholders en términos teóricos y prácticos. academia embargo relación TSX. . (TSX) 10 200 2019 contribuciones prácticos (TSX 1 20 201 2

ABSTRACT The different stakeholder groups and their influence on value distribution have received increasing attention from organizations and academia. In the recent stakeholder literature, there has been a theoretical development on value distribution, considering the attributes of strategic importance and power; however, the literature still lacks empirical studies to analyze and understand the relationship. This paper examines the association between stakeholders’ power and strategic importance and the value distribution to them by publicly traded companies on the Toronto Stock Exchange (TSX). To obtain relevant information on companies’ treatment of their stakeholders, we analyzed the content of 104 prospectuses for the TSX IPO process from 2008 to 2019. The results reveal that power and strategic importance are relevant to the distribution of value to stakeholders and that the strategic importance of stakeholders has a more significant influence than their power. Regarding contributions, our study advances previous debates in stakeholder literature in theoretical and practical terms. academia however relationship TSX. . (TSX) 10 200 2019 contributions terms (TSX 1 20 201 2

RESUMO Os diferentes grupos de stakeholders e seus aspectos de influência na alocação de valor vêm ganhando crescente demanda por parte das organizações e da academia. Na literatura recente de stakeholders, há um desenvolvimento teórico sobre distribuição de valor considerando atributos de importância estratégica e poder; no entanto, a literatura ainda carece de estudos empíricos para analisar e compreender a relação. Este trabalho tem como objetivo verificar a associação entre poder e importância estratégica dos stakeholders e a distribuição de valor a eles por empresas de capital aberto na Bolsa de Valores de Toronto (TSX). Para obter as informações relevantes sobre o tratamento dado pelas empresas aos seus stakeholders, analisamos o conteúdo de 104 prospectos para o processo de IPO da TSX de 2008 a 2019. Os resultados revelaram que o poder e a importância estratégica são relevantes para a distribuição de valor aos stakeholders e que a importância estratégica dos stakeholders tem uma influência mais significativa quando comparada ao seu poder. Quanto às contribuições, o nosso estudo avança debates anteriores na literatura de stakeholders em termos teóricos e práticos. academia entanto relação TSX. . (TSX) 10 200 2019 contribuições práticos (TSX 1 20 201 2

Abstract High infestations of epibiont ciliates on vertebrates or invertebrates are normally related to aquaculture tanks or similar environments, and the importance of this relationship in natural habitats is often disregarded. Here, we describe the first record of high infestation of ciliates on Aegla serrana in South America and conduct a brief morphological and phylogenetic characterization of these ciliates. Our findings confirm that cases of high infestation of ciliates on metazoans can indeed occur in natural environments. environments disregarded Here

Abstract Studies have highlighted melanoma immunogenicity, and the prognostic importance of tumor infiltrating lymphocytes (TILs) and mechanisms of tumor immune evasion, such as hyperexpression of programmed cell death ligand 1 (PDL-1). High endothelial venules (HEV) are specialized blood vessels that can facilitate the lymphocytes migration to the tumor. Here we evaluate the association of HEV density and PDL-1 expression in primary cutaneous melanomas with the presence and degree of TILs and with other clinicopathological variables (age, sex, tumor location, melanoma histological type, Breslow thickness, ulceration, regression signs, mitotic index). HEV density and PDL-1 expression were assessed immunohistochemically in 78 melanoma cases, using a specific antibody, and were detected in 59% and 76% of these, respectively. Positive associations were identified between HEV density and PDL-1 expression with the presence and degree of lymphocytic infiltration, melanoma histological type and ulceration presence. No correlation was found between HEV density and PDL-1 expression. Our findings confirm the HEV role in the recruitment and facilitation of lymphocyte transport in cutaneous melanomas, where HEV density is strongly associated with the degree of TILs. Additionally, PDL-1 hyperexpression suggests a possible mechanism of tumor immune evasion, which may lead to inactivation and reduction of the tumor lymphocytes number. immunogenicity (TILs evasion PDL1. PDL1 PDL . (PDL-1) (HEV PDL- age, age (age sex location thickness signs index. index index) 7 cases antibody 59 76 these respectively infiltration Additionally number (PDL-1 5 (PDL- (PDL

Abstract The present work is concerned with the use of a Response Surface Model of the reduced flexibility matrix for structural damage identification. A Response Surface Model (RSM) is fitted with the aim at providing a polynomial relationship between nodal cohesion parameters, used to describe the damage field within the structure, and elements of the reduced flexibility matrix. A design of experiment built on combinations of a relatively small number of nodal cohesion parameters is used to fit the RSM. The damage identification problem is formulated within the Bayesian framework and the Delayed Rejection Adaptive Metropolis method is used to sample the posterior probability density function of the uncertain cohesion parameters. Numerical simulations addressing damage identification in plates were carried out in order to assess the proposed approach, which succeeded in the identification of the different damage profiles considered. Besides, the use of a RSM, instead of a FEM of the structure, resulted in reductions of up almost 78% in the required computational cost. RSM (RSM structure approach considered Besides 78 cost 7

Abstract The current study proposes to investigate the diversity and phylogeny of trypanosomes parasitizing wild birds from the Brazilian Atlantic Forest. Cytological examination was carried out by light microscopy of blood smears and positive birds were selected for amplification of the 18S rDNA sequence through PCR. The resulting amplicons were subjected to purification, cloning, and sequencing analysis. Phylogenetic reconstruction was conducted, including all avian trypanosomes representative’s lineages. A total of ten bird samples from species of Turdus flavipes (N=1/12), T. albicollis (N=1/8), Tachyphonus coronatus (N=6/121), Thamnophilus caerulescens (N=1/22) and Synallaxis spixi (N=1/8) were positive for Trypanosoma spp. In the six specimens of T. coronatus, five distinct lineages of Trypanosoma spp. 18S-rRNA were observed in ninety sequences obtained, and using the strategy of cloning independent PCR, it was possible to observe that two of them were related to T. avium (JB01/JB02), and three were closed related to T. bennetti (JB03/ JB04/JB05). Addionaly, all fifteen sequences obtained from T. caerulescens/ S. spixi/T. flavipes/T. albicollis were identical. The present research is the first study to access molecular diversity and polyparasitism by avian trypanosomes in Brazil. The current research exhibits the wide genetic variability in avian trypanosomes and its non-specific relationship with its avian hosts. Forest S PCR purification analysis conducted representatives representative s N=1/12, N112 N N=1/12 , 1 12 (N=1/12) T N=1/8, N18 N=1/8 8 N=6/121, N6121 N=6/121 6 121 (N=6/121) N=1/22 N122 22 (N=1/22 (N=1/8 spp 18SrRNA SrRNA rRNA JB01/JB02, JB01JB02 JBJB JB01/JB02 JB01 JB02 JB (JB01/JB02) JB03/ JB03 (JB03 JB04/JB05. JB04JB05 JB04/JB05 . JB04 JB05 JB04/JB05) Addionaly spixiT spixi/T flavipesT flavipes/T identical Brazil nonspecific non specific hosts N11 N=1/1 (N=1/12 N1 N=1/ N612 N=6/12 (N=6/121 N=1/2 N12 2 (N=1/2 (N=1/ JB01JB0 JB01/JB0 JB0 (JB01/JB02 (JB0 JB04JB0 JB04/JB0 (N=1/1 N=1 N61 N=6/1 (N=6/12 (N=1 JB01JB JB01/JB (JB01/JB0 (JB JB04JB JB04/JB N= N6 N=6/ (N=6/1 (N= (JB01/JB N=6 (N=6/ (N (N=6

Abstract Over two decades, the area with sugarcane has more than doubled, from 4.8 million hectares in 2000 to 10 million in 2018, in Brazil. São Paulo State is mostly responsible for the sugarcane production in the country, accounting for 51% of the national production. In 2008, a study was conducted analysing the relationship between sugarcane cultivation and the aquatic macroinvertebrate community, showing the impacts of sugarcane on the macroinvertebrate aquatic fauna. The present study aims to gather actual information on the aquatic macroinvertebrate community in the same streams studied in 2008, to make a historical comparison with studies previously carried out. Eight streams were selected; four located in areas of sugarcane cultivation and four located in preserved areas. Three samples were carried out between 2018 and 2020. The aquatic macroinvertebrates were collected using a D-frame aquatic net (250 μm) including riffle and pools areas and identified using specific identification keys. The results of the historical assessment showed better ecological conditions of the streams in 2008 when compared to 2018 in areas of sugarcane cultivation, suggesting that the environmental impact was maintained and increased after ten years. decades doubled 48 4 8 4. 200 1 Brazil country 51 fauna selected 201 2020 Dframe D frame 250 (25 μm keys years 20 5 202 25 (2 2 (

Abstract The aim of this study is to analyze the waterborne metal levels in four lakes (one endorheic and three exorheic) of Harmony Point, Nelson Island, Antarctica. Water samples were analyzed by using a quadrupole type inductively coupled plasma mass spectrometer and an inductively coupled plasma optical emission spectrometer. The levels of As, Cu, Mn, Mo, and V were significantly lower and those of Cr, Mg, Na, and Sr were significantly higher in the endorheic lake than in the other lakes. Most water samples presented levels of Ag, Be, Cd, Pb, Se, Tl, and U below the limit of quantification, while for Ba, Co, and Ni around half of the samples were below this limit. The waterborne metal levels were not significantly different between the exorheic lakes. Waterborne metal levels in the freshwater lakes from Harmony Point did not show any clear relationship with their levels in the soil of the region or with bird guano, and overall, their levels indicate an environment without anthropogenic influence. Apparently, the Na levels are influenced by salt spray from the ocean, as they are related to the distance of the lakes from the ocean. one Island Antarctica As Cu Mn Mo Cr Mg Ag Be Cd Pb Se Tl quantification Ba Co guano overall influence Apparently ocean