Resumen El Repositorio Nacional de Materiales Orales consiste en una base de datos electrónica y una serie de interfaces de captura y consulta de información en línea. En este trabajo se abordarán los antecedentes del Repositorio con las discusiones teóricas y prácticas que permitieron su elaboración. También se presentará una breve revisión de su desarrollo y se tratarán algunos aspectos vinculados a su funcionalidad.

Abstract The National Repository of Oral Materials is an electronic database and a series of interfaces for capturing and consulting information online. In this work the antecedents of the Repository will be approached with the theoretical and practical discussions that allowed its elaboration. A brief review of its development will also be presented, and some aspects related to its functionality will be discussed.

Résumé Le Dépôt National de Documents Oraux se compose d'une base de données électronique et d'une série d'interfaces de saisie et de consultation d'informations en ligne. Ce document aborde l'historique du dépôt et les discussions théoriques et pratiques qui ont conduit à son développement. Il présentera également une brève revue de son développement et discutera de certains aspects liés à sa fonctionnalité.

RESUMEN El artículo propone una reflexión teórica -apoyada en antecedentes empíricos- que busca pensar las tensiones reconocibles en torno al trabajo con objetos y archivos digitales en la actualidad. Se parte de dos interrogantes: en primer término, ¿cómo dar cuenta del impacto de la digitalización en las prácticas de archivo que involucran formas específicas de clasificación, conservación, acceso y desecho de los objetos culturales? En segundo lugar, ¿cómo pensar la naturaleza de los objetos y archivos que nacen digitales y para circular en red? A partir de dichos interrogantes, el artículo aborda dos dimensiones de análisis: por un lado, el recorrido de investigación atravesado en torno a la producción de artistas, escritores e intelectuales; por el otro, las mutaciones que funden el problema posmoderno del archivo con el campo de la comunicación, los medios y las redes de información. La reflexión, entre teoría y prácticas, lleva a indagar la naturaleza de los objetos digitales en red, las formas actuales de producir y hacer circular conocimientos, y los cambios en los modos de registro e intervención documental.

ABSTRACT The article proposes a theoretical reflection -supported by empirical background- that seeks to think about the recognisable tensions around working with digital objects and archives today. Is based on two questions: firstly, how to account for the impact of digitisation on archival practices that involve specific forms of classification, preservation, access and disposal of cultural objects? Secondly, how to think about the nature of objects and archives that are born digital and networked? On the basis of these questions, the article addresses two dimensions of analysis: on the one hand, the research route traversed around the production of artists, writers and intellectuals; on the other hand, the mutations that merge the postmodern problem of the archive with the field of communication, the media and information networks. The reflection, between theory and practice, leads to an investigation of the nature of networked digital objects, the current forms of producing and circulating knowledge, and the changes in the modes of recording and documentary intervention.

RESUMO O artigo propõe uma reflexão teórica -apoiada em fundamentação empírica- que busca pensar sobre as tensões reconhecíveis acerca do trabalho com objetos e arquivos digitais hoje. Parte de duas questões: em primeiro lugar, como explicar o impacto da digitalização nas práticas de arquivo que envolvem formas específicas de classificação, conservação, acesso e descarte de objetos culturais? Em segundo lugar, como pensar a natureza dos objetos e arquivos que nascem digitais e circulam na rede? A partir dessas questões, o artigo aborda duas dimensões de análise: 1. O percurso de pesquisa atravessado acerca da produção de artistas, escritores e intelectuais; 2. As mutações que fundem o problema pós-moderno do arquivo com o campo da comunicação, os meios de comunicação e as redes de informação. A reflexão, entre teoria e práticas, leva a investigar a natureza dos objetos digitais em rede, as formas atuais de produção e circulação de conhecimento e as mudanças nos modos de registro e intervenção documental.

Abstract The IV Belém Summit in Pará, Brazil, held in August 2023, brought together the leaders of the member countries of the Amazon Cooperation Treaty Organization (OTCA) to discuss a new sustainable agenda for the region. The Amazon was the focus of attention due to its global importance as the largest watershed and the most extensive and diverse tropical forest on the planet. The Summit highlighted the importance of strengthening OTCA as the only intergovernmental coordination mechanism to implement the agreed presidential agenda. The Belém Declaration established priorities for effective regional cooperation, including sustainability, environmental preservation, and inclusive development. The challenges to be faced include the efficient management of OTCA, the improvement of governance and positioning as a center for regional and international technical and political cooperation. The successful implementation of the agreed agenda will require the commitment of the Amazon countries and the active participation of all stakeholders, especially indigenous peoples and local communities.

Resumen La IV Cumbre de Belém de Pará, Brasil, celebrada en agosto de 2023, reunió a personas líderes de los países integrantes de la Organización del Tratado de Cooperación Amazónica (OTCA), con el fin de discutir una nueva agenda sostenible para la región. La Amazonía fue el centro de atención debido a su importancia mundial como la cuenca hidrográfica más vasta y el bosque tropical más extenso y diverso del planeta. La Cumbre destacó la importancia de fortalecer la OTCA, como el único mecanismo intergubernamental de coordinación para implementar la agenda presidencial acordada. La Declaración de Belém estableció prioridades para una cooperación regional efectiva, incluyendo la sostenibilidad, la preservación ambiental y el desarrollo inclusivo. Los retos por enfrentar incluyen la gestión eficiente de la OTCA, la mejora de la gobernanza y el posicionamiento como un centro de cooperación técnica y política regional e internacional. La implementación exitosa de la agenda acordada requerirá el compromiso de los países amazónicos y la participación activa de todas las partes interesadas, especialmente, los pueblos indígenas y las comunidades locales.

ABSTRACT Community gardens inspired by agroecological models are trends in cities that seek to stimulate positive environments. Among the possible strategies, they allow us to integrate policies that aim to eradicate eradicate hunger, guarantee food quality and increase the family economy. This study aims to analyze the symbolic and emotional meanings of users during their activities in urban community gardens. There were 40 interviewees with average age between 45 and 80 years old, through the Affective Map Generator Instrument. The research was carried out on site and the participants were invited to develop the information and respond to the interview. The images produced are analyzed from their structures, feelings and senses. It is confirmed that experiences in these environments are mediated by feelings of pleasantness, belonging and restoration. Among the senses attributed, the connection with nature and social interaction stand out. It is concluded that these dimensions present in the studied gardens can contribute to the promotion of health, sustainability and environmental preservation.

RESUMO As hortas comunitárias inspiradas em modelos agroecológicos já são tendências nas cidades que buscam estimular ambientes positivos. Entre as possíveis estratégias, permitem integrar políticas que visam à erradicação da fome, a garantia de qualidade alimentar e o aumento da economia familiar. O presente estudo objetiva analisar os significados simbólicos e afetivos dos usuários diante de suas atividades nas hortas comunitárias urbanas. Foram 40 entrevistados com idade média entre 45 e 80 anos, por meio do Instrumento Gerador de Mapas Afetivos. A pesquisa foi realizada in loco e os participantes foram convidados a desenhar a horta e responder à entrevista. As imagens produzidas foram analisadas a partir de suas estruturas, sentimentos e sentidos. Constatou-se que as vivências nesses ambientes foram mediadas por sentimentos de agradabilidade, pertencimento e restauração. Entre os sentidos atribuídos, destacaram-se a conexão com a natureza e a interação social. Conclui-se que tais dimensões presentes nas hortas estudadas podem contribuir para a promoção da saúde, da sustentabilidade e a preservação ambiental.

ABSTRACT Physical activity is a right of every human being, it affects the strengthening of conditions for work; transformation of nature; the struggle and preservation of peace; in the physical-functional development of the individual and the formation of character; ethical, moral and volitional qualities in the individual and social. The research aims to assess the possibilities of physical activity to favor social skills in students with intellectual disabilities, by contributing to the promotion of a healthy generation, physically prepared according to their potentialities, their development needs corrective work and/or compensatory that improves and increases the possibilities of movements, towards the development of social relations in the contexts where it develops; For its development, theoretical, empirical and mathematical statistical methods were used, which made it possible to verify the declared scientific problem from the characterization of the selected sample, from the determination of the dimensions and indicators that allowed arriving at regularities, a great importance is attributed to it. educational value by exploring the environment and developing communicative relationships, as well as, they can build topological, temporal and spatial notions in different situations of movement, it also places the student with intellectual disability in situations of interaction with others, and requires him to seek resources or help to solve the activity, this favors relationships with others and achieve independent action.

RESUMEN La actividad física es un derecho de todo ser humano, repercute en el fortalecimiento de condiciones para el trabajo, en la transformación de la naturaleza, en la la lucha y preservación de la paz, en el desarrollo físico-funcional del individuo y en la formación del carácter; cualidades éticas, morales y volitivas en lo individual y social. La investigación tiene como objetivo valorar las posibilidades de la actividad física para favorecer las habilidades sociales en los educandos con discapacidad intelectual, al contribuir a la promoción de una generación saludable, físicamente preparada según sus potencialidades; su desarrollo necesita de un trabajo correctivo y/o compensatorio que perfeccione y aumente las posibilidades de movimientos hacia el desarrollo de las relaciones sociales en los contextos donde se desarrolla. Para su desarrollo se utilizaron métodos teóricos, empíricos y estadístico-matemáticos, los que posibilitaron la constatación del problema científico declarado a partir de la caracterización de la muestra seleccionada, desde la determinación de las dimensiones e indicadores que permitieron arribar a regularidades. A estos métodos se les atribuye un gran valor educativo al explorar el entorno y desarrollar las relaciones comunicativas, así como que pueden construir nociones topológicas, temporales y espaciales en diferentes situaciones de movimiento; además, sitúa al educando con discapacidad intelectual en situaciones de interacción con los demás, y le exige buscar recursos o ayuda para solucionar la actividad, lo que favorece las relaciones con los demás y logra una actuación con independencia.

RESUMO A atividade física é um direito de todo ser humano, tem impacto no fortalecimento das condições de trabalho, na transformação da natureza, na luta e preservação da paz, no desenvolvimento físico-funcional do indivíduo e na formação. personagem; qualidades éticas, morais e volitivas em nível individual e social. A pesquisa tem como objetivo avaliar as possibilidades da atividade física para promover habilidades sociais em alunos com deficiência intelectual, contribuindo para a promoção de uma geração saudável, preparada fisicamente de acordo com o seu potencial; O seu desenvolvimento requer um trabalho corretivo e/ou compensatório que aperfeiçoe e aumente as possibilidades de movimentos em direção ao desenvolvimento das relações sociais nos contextos onde se desenvolve. Para o seu desenvolvimento foram utilizados métodos teóricos, empíricos e estatístico-matemáticos, que permitiram verificar o problema científico declarado a partir da caracterização da amostra selecionada, a partir da determinação das dimensões e indicadores que permitiram chegar às regularidades. A esses métodos é atribuído grande valor educativo na exploração do ambiente e no desenvolvimento de relações comunicativas, bem como podem construir noções topológicas, temporais e espaciais em diferentes situações de movimento; Além disso, coloca o aluno com deficiência intelectual em situações de interação com outras pessoas e exige que ele busque recursos ou ajuda para resolver a atividade, o que favorece o relacionamento com os outros e alcança a ação independente.

Resumen Los concentrados de glóbulos rojos son el componente que se utiliza con mayor frecuencia en las transfusiones sanguíneas. La irradiación gamma de la unidad transfusional está indicada cuando un paciente corre el riesgo de desarrollar la enfermedad de injerto contra huésped asociada a una transfusión. Sin embargo, esta irradiación exacerba la lesión de almacenamiento aumentando los niveles plasmáticos de potasio y disminuyendo los de sodio. Estos efectos sobre los glóbulos rojos reducen el tiempo de viabilidad de las unidades transfusionales irradiadas almacenadas. En este trabajo, estudiamos el efecto de diferentes dosis de radiación gamma sobre la concentración de los iones plasmáticos en las bolsas irradiadas, los cuales son indicativos de posibles alteraciones de las membranas de los glóbulos rojos y de los leucocitos. El análisis de los resultados obtenidos muestra que la radiación, a las dosis estudiadas, no afecta el consumo de glucosa. Sin embargo, la permeabilidad de la membrana de los eritrocitos aumenta con la dosis de radiación, indicando que este tipo de alteraciones podrían ser dosis dependiente. Estos resultados podrían ayudar a diseñar nuevos medios de conservación para reducir el daño a la membrana inducido por la radiación.

Abstrac Red blood cell concentrates are the most widely used component in blood transfusions. Gamma irradiation of the transfusion unit is indicated when a patient is at risk of developing transfusion-associated graft-versus-host disease. However, this irradiation exacerbates storage damage by increasing potassium and decreasing sodium plasmatic levels. These effects on red blood cells reduce the viability time of stored irradiated units. In this work, we study the different radiation dose effects on the concentration of plasmatic ions in the gamma-irradiated bags, which are attributed to possible membrane alterations of red blood cells and leukocytes. The result analysis shows that radiation, at the doses studied, does not affect glucose consumption. However, the permeability of the erythrocyte membrane increases with the radiation dose, indicating that these types of alterations could be dose-dependent. These results could help to design a new preservation medium to reduce radiation-induced membrane damage.

Abstract In Brazil, 65% of the electric power production comes from hydroelectric power plants (HPPs). In some cases, these power plants divert the course of rivers, resulting in regions impacted by the absence of water, which are known as reduced fow sections (RFS). These sections are regulated by legislation and aim to maintain the minimum fow necessary to preserve the fauna, flora, fish, and human consumption in the region. Depending on the time of year, this minimum flow, also known as ecological flow (residual flow), can reach significant values, resulting in the interruption of power generation in these HPPs. This article presents a study case of two hydroelectric power plants located in different Brazilian states. The objective of this study is to explore the feasibility of implementing auxiliary generator groups that can operate uninterruptedly at different drop heights while ensuring the preservation of residual flow during low flow periods or taking advantage of the overflow flow by spillways during high flow periods. This auxiliary system would use the same set of structures and transmission systems as the main power plant, minimizing environmental impacts and implementation costs, allowing this solution to be implemented in HPPs that face this type of problem in Brazil. The results obtained indicate that HPPs with larger reservoirs have a greater ability to maintain residual flows with minimal generation loss and that run-of-the-river power plants are the most impacted by the maintenance of residual flow.

The complexity of root canal morphology can influence cleaning and shaping of the root canal system and, ultimately, treatment outcomes. Undiscovered root canal morphology can greatly reduce the prognosis of endodontic treatment due to the preservation of offending organisms and their byproducts. The maxillary first molar has been identified as one of the most challenging teeth to treat endodontically due to its complex internal anatomy, the presence of additional and accessory canals and intricate root canal configurations. This paper is the second of two that provide a micro-computed tomography (micro-CT)-focused overview of available literature on various aspects of the root and canal morphology of the maxillary first permanent molar. The aim of this paper is to provide an overview of relevant aspects of the internal root morphology in different populations. The content is supported by illustrative micro-CT images and clinical cases or images.

ABSTRACT: Jaw cysts are the most common bone lesions in the maxillofacial region. Enucleation of the lesions along with the primary closure of the defects are the optimal treatment nowadays. On some occasions, the resulting bone defect can be so large that it affects the stability of neighboring teeth, compromises the integrity of the bone, or produces a delayed healing that can even prevent complete bone regeneration. It is considered that the lack of regeneration exposes the patient to risk of infection, delay of rehabilitation treatments in areas of high aesthetic demand and loss of dental vitality. To reduce the risk of alterations in complete bone regeneration of cystic cavities, the possibility of filling these defects with bone grafts or other alveolar preservation techniques to promote healing, has been proposed after cyst enucleation. Theoretically, the use of these grafts improves the quality and decreases the bone healing time, allowing the patient to quickly recover the usual functions of the dentoalveolar component, limiting the period of postoperative care that restricts eating, sports or oral rehabilitation. The present work aims to carry out a review of the literature regarding the benefits of the use of bone grafts in the surgical treatment of maxillary cysts and to present a clinical case with the surgical details of this technique.

RESUMEN: Los quistes de los maxilares son las lesiones óseas más comunes en la región maxilofacial. La enucleación de las lesiones y el cierre primario de los defectos, son en conjunto, el tratamiento óptimo hoy en día. En algunas ocasiones el defecto óseo resultante puede ser de un tamaño tan grande que afecta la estabilidad de dientes vecinos, comprometa la indemnidad del hueso o produce un retraso cicatrizal que incluso puede impedir una regeneración ósea completa. Se considera que esta falta de regeneración expone al paciente a riesgos de infección tardía, retraso de los tratamientos rehabilitadores en zonas de alta demanda estética y pérdida de vitalidad dentaria. Para disminuir el riesgo de alteraciones en la regeneración ósea completa de cavidades quísticas, se ha propuesto la posibilidad de que tras la enucleación del quiste se rellenen estos defectos con injertos óseos u otras técnicas de preservación alveolar para favorecer la cicatrización. Teóricamente el uso de estos injertos mejora la calidad y disminuye el tiempo de cicatrización ósea, permitiendo que el paciente recupere rápidamente las funciones habituales del componente dentoalveolar, acortando el periodo de cuidados postoperatorios que restringen la alimentación, los deportes o la rehabilitación oral. El actual trabajo tiene como objetivo realizar una revisión de la literatura respecto a los beneficios del uso de injertos óseos en el tratamiento quirúrgico de los quistes maxilares y presentar un caso clínico con los detalles quirúrgicos de esta técnica.

Tomatoes are an important and versatile crop with a short shelf life. Postharvest losses due to fruit decay and handling are reportedly as high as 30-50% globally; therefore, the agricultural sector would benefit from solutions that target the preservation of crops such as tomatoes. In this study, we investigated the potential use of sulfur dioxide (SO2) to provide postharvest protection against fungal decay in tomatoes whilst maintaining the quality of tomato fruit. Three tomato varieties packed as bulk (3-5 kg) cartons were exposed to SO2-generating sheets applied as either a top sheet over the fruit or a bottom sheet placed on the base of the carton before packing the fruit on the top. The results show that the application of SO2-generating sheets reduced the natural progression of decay on 'Roma' tomatoes by up to 60% and up to 80% on 'Rosa' tomatoes. Only marginal decay control was observed on 'Round' tomatoes. The top sheet application rendered the best results, as the application of the bottom sheet resulted in phytotoxicity in the form of SO2 damage. SO2 application reduced fruit shrivelling, especially when tomatoes were stored at higher temperatures during their shelf life. The application of SO2 on the tomatoes did not have any negative effects on fruit firmness. The results of this study provide a case to further explore the use of SO2-generating sheets on tomatoes to prevent postharvest decay. Differences in varietal physiology may be key to the successful application of this technology. SIGNIFICANCE: Tomatoes are a crop that is highly susceptible to postharvest decay. These effects lie mainly with the end consumer. Besides the monetary loss of buying a commodity that rots quickly, pathogens infecting tomatoes are known to produce mycotoxins that pose a risk to human health. With food availability and safety concerns, the application of a product that could reduce these concerns would be beneficial to the agricultural sector.

RESUMEN La diversidad cultural y el patrimonio desempeñan un papel fundamental en la identidad y riqueza de las comunidades en el mundo. Estas, emergen como un tesoro cultural cuya preservación y promoción se han convertido en aspectos cruciales para el desarrollo sostenible y la conservación de su identidad. El trabajo tiene como objetivo fortalecer el patrimonio cultural inmaterial e implantar una ruta turística en la comunidad Tsáchila. Para el estudio se utilizó el método general de solución de problemas y el PESTEL, para identificar las causas que amenazan la preservación del patrimonio cultural. Se concluye que se deben implementar estrategias que requieren la colaboración entre la comunidad Tsáchila y las autoridades locales. Además, desarrollar programas de educación y proyectos de concienciación, mientras se fomenta la participación activa de la comunidad en la preservación de sus tradiciones.

ABSTRACT Cultural diversity and heritage play a fundamental role in the identity and wealth of communities around the world. These emerge as a cultural treasure whose preservation and promotion have become crucial aspects for sustainable development and the conservation of its identity. The work aims to strengthen the intangible cultural heritage and implement a tourist route in the Tsáchila community. For the study, the general problem-solving method and PESTEL were used to identify the causes that threaten the preservation of cultural heritage. It is concluded that strategies that require collaboration between the Tsáchila community and local authorities must be implemented. In addition, develop education programs and awareness projects, while encouraging the active participation of the community in the preservation of its traditions.

Toponymy safeguards this valuable blend of information and knowledge present in the layers ofplace names, as it retrieves crystallized meanings. Proceeding from the hypothesis that locatives are fossils, attributing to them the condition of remaining intact, surviving to the present day, allowing us to delve into the past of this region. We have proposed to document the lexical-historical-cultural heritage of the toponymy of Mariana and its districts through the following methodology: (I) verify if these toponyms existed even before being elevated to such condition; (II) recover part of the intangible cultural heritage present in the explanation by the informant about the origin of the place name where they live; (III) understand the motivation, formation, and oldest meaning of the toponym; (IV) investigate if there have been any alterations regarding the written form (spelling). This present work aims at the knowledge, recovery, and preservation of the collective memory present in the names of places, as these have been replaced by other forms that, in some cases, endure until the present day. The choice of this toponym is justified by the evident relationship between linguistic and cultural elements, as they indicate associative relationships when named.

A Toponímia custodia essa poderosa mistura de informações e conhecimentos presentes nas camadas toponímicas, uma vez que recupera os significados cristalizados. Partindo da hipótese de que os locativos são fósseis e isso atribui a eles uma condição de permanecerem intactos sobrevivendo até os tempos atuais, permitindo-nos penetrar no passado dessa região. Propomos-nos documentar o patrimônio léxico-histórico-cultural da toponímia de Mariana e de seus distritos, por meio da seguinte metodologia: (I) verificar se esses topônimos já existiam mesmo antes de serem elevados a tal condição; (II) recuperar parte do patrimônio cultural imaterial presente na explicação por parte do informante sobre a origem do nome do lugar onde mora (III) compreender a motivação e formação, o significado mais antigo do topônimo (IV) investigar se houve alguma alteração relativa à forma escrita (grafia). O presente trabalho tem como objetivo o conhecimento, a recuperação e a preservação da memória coletiva presentes nos nomes de lugares, uma vez que esses foram substituídos por outras formas que, em alguns casos, sobreviveram até os dias atuais. A escolha desse topônimo justifica-se pela flagrante relação entre o linguístico e o cultural, já que indicam relações associativas ao serem nomeadas.

Abstract Currently, the food industry prioritizes the safety and quality of products, facing the challenge of maintaining sensory and nutritional integrity. To address this challenge, emerging non-thermal technologies are being explored that offer significant advantages in improving food quality over conventional technologies. This review aims to provide a comprehensive understanding of how these innovative technologies can transform the traditional food processing landscape as we know it. Non-thermal technologies have the potential to reduce energy consumption for food production, processing and packaging, compared to conventional thermal treatments. Furthermore, these technologies are particularly suitable for efficiently preserving bioactive compounds present in foods. This review describes the fundamental principles, applications, advantages and limitations of emerging technologies in food processing. These technologies include pulsed electric field, ultrasound, cold plasma, high pressure, irradiation, light-emitting diodes, pulsed light and oscillating magnetic fields. Despite the large number of studies on the subject, more research is required to optimize and improve the efficiency of the application of these technologies, alone or in combination, in food processing. The bioaccessibility and bioactivity of the compounds, nutritional value, shelf life and sensory aspects may be variables of interest.

Resumen En la actualidad, la industria alimentaria da prioridad a la seguridad y calidad de los productos, enfrentándose al desafío de mantener la integridad sensorial y nutricional. Para abordar este desafío, se vienen explorando tecnologías no térmicas emergentes que ofrecen ventajas significativas en la mejora de la calidad de los alimentos frente a las tecnologías convencionales. Esta revisión tiene como objetivo proporcionar una comprensión integral de cómo estas tecnologías innovadoras pueden transformar el panorama tradicional del procesamiento de alimentos tal como lo conocemos. Las tecnologías no térmicas tienen el potencial de reducir el consumo de energía destinado a la producción, procesamiento y envasado de alimentos, en comparación con los tratamientos térmicos convencionales. Además, estas tecnologías son particularmente adecuadas para preservar eficientemente los compuestos bioactivos presentes en los alimentos. En esta revisión se describen los principios fundamentales, aplicaciones, ventajas y limitaciones de las tecnologías emergentes en el procesamiento de alimentos. En estas tecnologías se incluyen, campo eléctrico pulsado, ultrasonido, plasma frío, alta presión, irradiación, diodos emisores de luz, luz pulsada y campos magnéticos oscilantes. A pesar de la gran cantidad de estudios respecto al tema, se requiere mayor investigación para optimizar y mejorar la eficiencia de la aplicación de estas tecnologías, solas o en combinación, en el procesamiento de alimentos. La bioaccesibilidad y bioactividad de los compuestos, el valor nutricional, la vida útil y los aspectos sensoriales pueden ser variables de interés.

Resumo Este estudo foi realizado para avaliar o efeito da Glutamina, como um dipeptídeo ou forma de aminoácido livre, na progressão de queimaduras em ratos. Trinta ratos Wistar machos foram queimados com um pente de metal aquecido em água fervente (98 °C) por três minutos, criando quatro áreas retangulares queimadas separadas por três interesespaços não queimados (zona de estase) em ambos os lados do dorso. Os animais foram randomizados em três grupos (n = 10): solução salina (G1-Controle) e grupos tratados que receberam glutamina via oral como dipeptídeo (G2-Dip) ou aminoácido livre (G3-FreeAA). Dois e sete dias após a queimadura, as lesões foram fotografadas para avaliação da evolução da necrose entre os espaços não queimados. Sete dias após a lesão, foi dosada a glutationa sérica e realizada análise histopatológica. Pelas fotografias, houve uma redução significativa na progressão da necrose no G3-Free-AA entre os dias dois e sete. A análise histopatológica no dia 7 mostrou uma zona de estase significativamente maior sem necrose e número mais elevado de fibroblastos em G2-Dip e G3-FreeAA em comparação com G1-Controle. Os níveis plasmáticos de glutationa foram maiores no G2-Dip em relação ao G1-Controle, e houve tendência a níveis mais elevados no G3-FreeAA. A redução das lesões histológicas, maior produção de fibroblastos, maior quantidade de glutationa podem ter beneficiado a evolução da necrose da queimadura, que mostrou maior preservação dos interespaços.

Abstract This study was carried out to evaluate the effect of Glutamine, as a dipeptide or a free amino acid form, on the progression of burn injuries in rats. Thirty male Wistar rats were burned with a comb metal plate heated in boiling water (98 °C) for three minutes, creating four rectangular full-thickness burn areas separated by three unburned interspaces (zone of stasis) in both dorsum sides. The animals were randomized into three groups (n=10): saline solution (G1-Control) and treated groups that orally received Glutamine as dipeptide (G2-Dip) or free amino acid (G3-FreeAA). Two and seven days after burn injury, lesions were photographed for unburned interspaces necrosis evolution assessment. Seven days after injury, glutathione seric was measured and histopathological analysis was performed. By photographs, there was a significant reduction in necrosis progression in G3-Free-AA between days two and seven. Histopathological analysis at day 7 showed a significantly higher stasis zone without necrosis and a higher number of fibroblasts in G2-Dip and G3-FreeAA compared with G1-Control. Also, glutathione serum dosage was higher in G2-Dip. The plasmatic glutathione levels were higher in the G2-Dip than the G1-Control, and there was a trend to higher levels in G3-FreeAA. The reduction in histological lesions, greater production of fibroblasts, and greater amounts of glutathione may have benefited the evolution of burn necrosis, which showed greater preservation of interspaces.

Resumo A esporulação de Didymella bryoniae in vitro é de grande importância para estudos que requerem inóculo puro e em grandes quantidades. Assim, os objetivos deste estudo foram identificar a melhor condição para esporulação de D. bryoniae combinando diferentes espectros de luz (luz UV-A ou UV-B, luz branca e escuro contínuo) com distintos meios de cultura (PDA, V8, ML e PDAB) e, avaliar a sobrevivência do fungo armazenado a -20°C ao longo do tempo. As amostras de fungo só esporularam quando submetidas ao tratamento com luz UV-B, independentemente do meio de cultura. Maior aparecimento de esporos do tipo conídio foi observado no meio PDAB, e a menor produção ocorreu no meio ML. Estruturas reprodutivas, como peritécios e picnídeos, foram observadas em todos os meios de cultura. No entanto, houve uma variação considerável na quantidade de cada estrutura entre os diferentes meios de cultura. Os meios ML e V8 apresentaram maior número de peritécios e os meios PDA e PDAB apresentaram maior proporção de picnídeos em relação aos peritécios. A duração do armazenamento a -20°C não afetou o crescimento micelial ou a taxa de crescimento micelial. Em conclusão, a luz UV-B é essencial para a esporulação de D. bryoniae in vitro. Além disso, a composição do meio de cultura influencia o tipo de estrutura fúngica produzida, bem como o tamanho e a quantidade dos esporos. O congelamento a -20°C é uma técnica eficiente que pode ser usada para armazenar D. bryoniae por pelo menos cinco meses sem perda de viabilidade

Abstract The in vitro sporulation of Didymella bryoniae is of great importance for studies that require pure inoculum and in large quantities. Thus, the objectives of this study were to identify the best condition for D. bryoniae sporulation combining different light spectra (UV-A or UV-B light, white light, and continuous dark), with distinct culture media (PDA, V8, ML, and PDAB) and, to evaluate fungus’ survivability stored at -20°C over time. The fungus samples were only able to sporulate when subjected to the UV-B light treatment, regardless of the culture medium. The highest appearance of spores conidium type was observed in the PDAB medium, and the lowest production occurred in the ML medium. Reproductive structures, such as perithecia and pycnidia, were observed in all culture media. However, there was considerable variation in the amount of each structure between the different culture media. The ML and V8 media showed a greater number of perithecia and the PDA and PDAB media presented a greater proportion of pycnidia compared to perithecia. The storage duration at -20°C did not affect mycelial growth or mycelial growth rate. In conclusion, the UV-B light is essential for D. bryoniae in vitro sporulation. Moreover, the culture medium composition influences the type of fungal structure produced, as well as spores’ size and quantity. Freezing at -20°C is an efficient technique that can be used to store D. bryoniae for at least five months without loss of viability.