RESUMO: A batata-doce é uma cultura importante para a segurança alimentar com mercado mundial em ascensão. Com base nas alterações fisiológicas das plantas em resposta às condições climáticas e à fertilização, este estudo avaliou os efeitos da época de cultivo (período chuvoso e seco) e de doses de P2O5 (0, 50, 100, 200 e 400 kg ha-1) na produtividade total e por classes comerciais, bem como, a composição química das raízes de armazenamento de duas cultivares de batata-doce. A produtividade de raízes tuberosas foi maior na estação chuvosa. As doses ótimas agronômica e econômica foram de 128 e 95 kg ha-1 de P2O5 para a cultivar Canadense. A cultivar Uruguaiana não respondeu a adubação fosfatada, mas suas raízes tiveram maiores teores de matéria seca, lipídios, fibras, açúcares totais e redutores e amido. O cultivo de batata-doce no período chuvoso com doses de até 100 kg ha-1 de P2O5 aumenta a produtividade de raízes em classes de maior valor econômico e com maiores teores de carboidratos. Estes resultados ajudam os produtores a planejarem o plantio e a colheita da batata-doce ao longo do ano e contribui para a gestão da aplicação de fertilizantes fosfatados. RESUMO batatadoce batata doce ascensão fertilização seco PO P P2O 0, 0 (0 50 20 40 ha1 ha 1 comerciais como batatadoce. doce. chuvosa 12 9 ha- Canadense fosfatada seca lipídios fibras amido 10 carboidratos fosfatados ( 5 2 4

ABSTRACT: Sweet potato is an important food security crop, and the world market for this root is on the rise. Based on the physiological changes in plants in response to climatic conditions and fertilization, this study evaluated the effects of the growing season (rainy and dry season) and P2O5 doses (0, 50, 100, 200 and 400 kg ha-1) on total yield, marketable classes yields, as well as chemical composition of storage roots of two sweet potato cultivars. The storage roots yield was greater in the rainy season. The optimum agronomic and economic doses were 128 and 95 kg ha-1 P2O5 for the cultivar Canadense. Cultivar Uruguaiana did not respond to phosphate fertilization, but its storage roots had higher contents of dry matter, lipids, fibers, total and reducing sugars, and starch. Sweet potato cultivation in the rainy season with doses up to 100 kg ha-1 P2O5 increase root yield in marketable size classes in higher economic value and with higher carbohydrate contents. The results can help producers schedule the planting and harvesting of sweet potatoes throughout the year and contribute to the seasonal management of phosphate fertilizer application. ABSTRACT crop rise fertilization PO P O P2O 0, 0 (0 50 20 40 ha1 ha 1 yields cultivars 12 9 ha- Canadense matter lipids fibers sugars starch 10 application ( 5 2 4

RESUMO O presente estudo visou investigar a força de atrito durante o processo de separação da batata do solo, bem como os fatores e padrões que influenciam essa força, além de estabelecer um sistema de teste para medir a força de atrito das batatas. Verificou-se que a massa da batata, a velocidade da manivela e a inclinação da peneira têm um impacto significativo na força de atrito ao longo da superfície da peneira. A interação entre a massa da batata e a velocidade da manivela tem o maior impacto na força de atrito ao longo da superfície da peneira. Além disso, sob os mesmos fatores - massa da batata entre 200-400 g, velocidade da manivela em 200 r min-1 e ângulo inclinado da peneira em 14.7°, a força de atrito das batatas ao longo da superfície com o solo é muito maior do que ao longo da superfície sem solo, variando entre 2,32 e 4,85. Verificouse Verificou se disso 200400 400 200-40 g 20 min1 min 1 min- 147 14 7 14.7° 232 2 32 2,3 485 4 85 4,85 20040 40 200-4 14.7 23 3 2, 48 8 4,8 2004 200- 14. 4,

ABSTRACT This study aimed to explore the friction force on potatoes in the process of potato-soil separation, its influencing factors and laws, and establish a testing system of potato-friction force. It was verified that the potato mass, crank speed, and sieve inclination significantly affected the potato’s friction force along the sieve surface. The interaction of potato mass and crank speed had the greatest effect on the friction force on the potato along the sieve surface. In addition, under the same factors of potato mass of 200~400 g, crank speed of 200 r min-1, and sieve inclination of 14.7°, the friction force of potato along the sieve surface with soil was much greater than that along the sieve surface without soil, ranging between 2.32 and 4.85. potatosoil separation laws potatofriction potatos s addition 200400 400 200~40 g 20 min1, min1 min 1, 1 min-1 147 14 7 14.7° 232 2 32 2.3 485 4 85 4.85 20040 40 200~4 min- 14.7 23 3 2. 48 8 4.8 2004 200~ 14. 4.

ABSTRACT In response to global concerns over food scarcity, the sweet potato (Ipomoea batatas (L.) Lam) has emerged as a promising alternative due to its rich composition of carbohydrates (starch), protein, fiber, vitamins, and minerals. This study aims to assess the physicochemical characteristics and nutritional content of different sweet potato landraces cultivated in Yucatan, Mexico. The study involved the evaluation of eight landraces of sweet potato cultivated in traditional farming systems, assessing both physicochemical and proximate composition variables, and determining the starch yield, starch color, protein content, swelling degree, and starch solubility index. The sweet potato landraces showed values ranging from acidic to slightly neutral pH (6-6.4). The yellow sweet potato II, purple sweet potato, and pink sweet potato landraces displayed high levels of total soluble solids with a range of 12.5 to 14.33 °Brix. Significant differences were observed in both peel and root pulp coloration. The pink, yellow, purple I, mamey, yellow II, and purple sweet potato landraces, presented elevated percentages of carbohydrates (85.0% to 89.0%), fats (1.1% to 1.5%), protein (3.5% to 5.1%), fiber (4.0% to 5.0%), and ash (3.5% to 4.0%). The mamey sweet potato and pink sweet potato landraces showed higher starch yields. Swelling power and starch solubility index peaked at a temperature of 90 °C. These results highlight the significance of sweet potato landraces cultivated in Yucatan as a valuable genetic resource with nutritional potential, which can be included in the diet of people, genetic improvement and species conservation programs.

Abstract Introduction. Sweet potato (Ipomoea batatas (L) Lam.) is sensitive to weed competition, and chemical control in Costa Rica is challenging due to limited local information on herbicide selectivity. Objective. To determine the selectivity of six herbicides applied at different stages of development to Criollo sweet potatoes and their effect on coverage, biomass, and crop yield. Materials and methods. Between August and December 2021, three experiments were conducted at the Fabio Baudrit Moreno Agricultural Experimental Station, Universidad de Costa Rica, Alajuela, Costa Rica. Herbicides were applied (in kg a.i. ha-1) as follows: a) Experiment 1: 0.36 of metribuzin, 1.50 of pendimethalin, 0.48 of clomazone, 0.075 of halosulfuron, 1.00 of linuron, and 1.25 of metolachlor, applied six days after planting (dap) in a randomized block design with four repetitions; b) Experiment 2: 1.00 of linuron and 0.36 of metribuzin 0.36, applied at -1, 15 and 30 dap, in a randomized block design with a 2x3 factorial arrangement and three repetitions; and c) Experiment 3: 0.075 of halosulfuron and 0.36 of metribuzin, mixed with 0.125 of fluazifop-p-butyl in a randomized block design with three repetitions. Weeding was performed at 15 and 30 dap in all cases. Results. Root yield was not affected in any of the three experiments. Experiment 1: Only halosulfuron caused mild damage to the plants; no herbicide affected above-ground biomass production. Experiment 2: Only linuron and metribuzin applied at 15 and 30 dap caused mild damage. Experiment 3: The mixtures caused temporary damage to the plants. Conclusions. The evaluated herbicides were selective to the Criollo sweet potatoes, and did not affect the yield of marketable storage roots. Only halosulfuron caused mild damage in all application forms, and metribuzin and linuron only in late applications.

Resumen Introducción. El camote (Ipomoea batatas (L) Lam) es sensible a la competencia por arvenses, cuyo control químico en Costa Rica se dificulta por la limitada información local generada sobre selectividad de herbicidas. Objetivo. Determinar la selectividad de seis herbicidas aplicados en diferentes etapas de desarrollo al camote Criollo y su efecto en la cobertura, biomasa y rendimiento del cultivo. Materiales y métodos. Entre agosto y diciembre 2021, se realizaron tres experimentos en la Estación Experimental Agrícola Fabio Baudrit Moreno, Universidad de Costa Rica, Alajuela, Costa Rica. Estos incluyeron la aplicación de herbicidas (en kg i.a. ha-1) de la siguiente manera: a) experimento 1: 0,36 de metribuzin, 1,50 de pendimetalina, 0,48 de clomazone, 0,075 de halosulfuron, 1,00 de linuron y 1,25 de metolaclor, aplicados seis días después de la siembra (dds) en diseño de bloques al azar con cuatro repeticiones; b) experimento 2: 1,00 de linuron y 0,36 de metribuzin, aplicados a los -1, 15 y 30 dds, en diseño de bloques al azar con arreglo factorial 2x3 y tres repeticiones; y c) experimento 3: 0,075 de halosulfuron y 0,36 de metribuzin, mezclado con 0,125 de fluazifop-p-butil en diseño de bloques al azar con tres repeticiones. En todos los casos se realizó deshierba 15 y 30 dds. Resultados. En ninguno de los tres experimentos se vio afectada la producción de raíces reservantes. Experimento 1: solo el halosulfuron causó daños leves en las plantas; ningún herbicida afectó la producción de biomasa aérea. Experimento 2: solo el linuron y metribuzin aplicados 15 y 30 dds causaron daños leves. Experimento 3: las mezclas causaron daños temporales en las plantas. Conclusiones. Los herbicidas evaluados fueron selectivos al camote variedad Criollo y no afectaron el rendimiento de raíces reservantes comercializables; solo halosulfuron causó daños leves en todas las formas de aplicación, y metribuzin y linuron solo en aplicaciones tardías.

Abstract Introduction. The parameters of physiological response to drought stress are the result of a combination of slow- or fast-acting attributes. Gas exchange variables are classified as fast-acting and their level of occurrence or impact depends on the interaction between factors such as genotype and the duration, intensity, and phenological stage of stress occurrence. Objective. To identify the levels of physiological response exhibited by Solanum phureja under progressive water deficit stress. Materials and methods. The experiment was conducted between 2019 and 2020 under semi-controlled conditions at the Obonuco Research Center of the Corporación Colombiana de Investigación Agropecuaria. Four potato cultivars were planted, with half of the trial was maintained at field capacity and the other half subjected to water deficit stress by withholding irrigation for 15 days, followed by rehydration. Gas exchange variables, chlorophyll content, and photosynthetic efficiency were evaluated every three days. Results. Significant differences were observed, with maximum gas exchange values in control plants showing 16.67 µmol m-2 s-1 photosynthesis rate (A); 0.34 mol m-2 s-1 stomatal conductance (gs); 5.5 mmol m-2 s-1 transpiration (E), and under stress, 1.17 µmol m-2 s-1 (A); 0.013 mol m-2 s-1 (gs); 0.29 mmol m-2 s-1 (E). Chlorophyll content values ranged between 451.7 and 474.69 mg m-2 with and without irrigation, respectively. Conclusions. Stomatic closure was the earliest response to water deficit, and potato plants showed recovery in gas exchange values after rehydration following stress. Finally, three levels of physiological response were identified: mild, moderate, and severe stress, depending on the intensity and duration of stress, which is useful for future studies and the development of irrigation schedules.

Resumen Introducción. Los parámetros de respuesta fisiológica al estrés por sequía son la conjugación de una serie de atributos de acción lenta o rápida. Las variables de intercambio gaseoso son catalogadas como de respuesta rápida, su nivel de aparición o afección dependen de la interacción entre factores como el genotipo y duración, intensidad y etapa fenológica de ocurrencia del estrés. Objetivo. Identificar los niveles de respuesta fisiológica que presenta Solanum phureja bajo el estrés progresivo por déficit hídrico. Materiales y métodos. El experimento se realizó entre 2019 y 2020, bajo condiciones semi-controladas en el Centro de Investigación Obonuco de la Corporación Colombiana de Investigación Agropecuaria. Se sembraron cuatro cultivares de papa, la mitad del ensayo se mantuvo con riego a capacidad de campo y a la otra parte se le suspendió para inducir estrés por déficit hídrico durante 15 días, luego se rehidrataron; se evaluaron variables de intercambio gaseoso, clorofila y eficiencia fotosintética cada tres días. Resultados. Se presentaron diferencias significativas con los valores máximos de intercambio gaseoso en plantas del tratamiento control con 16,67 µmol m-2 s-1 tasa de fotosíntesis (A); 0,34 mol m-2 s-1 conductancia estomática (gs) 5,5 mmol m-2 s-1 transpiración (E) y en estrés 1,17 µmol m-2 s-1 (A); 0,013 mol m-2 s-1 (gs); 0,29 mmol m-2 s-1 (E). En cuanto al contenido de clorofila, se presentaron valores entre 451,7 y 474,69 mg m-2 con riego y sin riego, respectivamente. Conclusiones. El cierre estomático fue la respuesta más temprana al déficit hídrico, además, las plantas de papa presentaron recuperación de los valores de intercambio gaseoso después del suministro de riego posterior al estrés. Finalmente, se identificaron tres niveles de respuesta fisiológica, estrés leve, moderado y severo, según la intensidad y duración del estrés, lo cual es de utilidad para futuros estudios y planteamientos de calendarios de riego.

Abstract Introduction. Endophytic fungi (EF) in grapevines (Vitis vinifera) have great potential for agricultural applications and may represent possible biological control agents for vineyards. Objective. To isolate and identify endophytic fungi (EF) from grapevine plants with antibiosis capacity against Botrytis cinerea. Materials and methods. Young and mature branches from forty healthy grapevine plants were collected from seven production lots in the Cañete province, Lima, Peru, during March to May 2022. EF isolation was performed on 702 sections of disinfected tissues using potato dextrose agar (PDA) culture medium, and identification was carried out at the genus level. Secondary metabolites were extracted from twenty-eight EF strains to determine the mycelial inhibition of the pathogen. An analysis of variance (ANOVA) was conducted on the evaluated parameters using the Minitab statistical program. Results. A total of 129 EF strains were isolated from leaves and stems of young and mature grapevine branches, encompassing seven genera (Trichoderma, Aspergillus, Alternaria, Cladosporium, Fusarium, Nigrospora, and Stemphylium). The highest frequency was found in leaves and mature branches. Trichoderma and Alternaria were the most frecuent genera in grapevine plants, with 22.48 % and 21.71%, respectively. Metabolites obtained from the EFVH-01 strain of the genus Cladosporium, and EF-99 and EF-70 strains of the genus Alternaria, had the greatest effect on mycelial inhibition of the pathogen. Conclusions. Strains of endophytic fungi from the genera Cladosporium and Alternaria showed antibiosis capacity against Botrytis cinerea at the laboratory scale, which could potential biocontrol agents of pathogens in grapevine cultivation.

Resumen Introducción. Los hongos endófitos (HE) de la vid (Vitis vinifera) tienen un gran potencial de aplicación en la agricultura y pueden representar posibles agentes de control biológico de los viñedos. Objetivo. Aislar e identificar los hongos endófitos (HE) de plantas de vid con capacidad de antibiosis hacia Botrytis cinerea. Materiales y métodos. Se colectaron ramas jóvenes y maduras de cuarenta plantas sanas de vid de siete lotes de producción en la provincia de Cañete, Lima, Perú, durante los meses de marzo a mayo 2022. El aislamiento de HE se realizó de 702 secciones de tejidos desinfectados en medio de cultivo papa dextrosa agar (PDA) y la identificación fue a nivel de género. Se extrajeron metabolitos secundarios de veintiocho cepas de HE para determinar la inhibición micelial del patógeno. Se llevó a cabo un análisis de varianza (ANDEVA) de los parámetros evaluados con el programa estadístico Minitab. Resultados. Se aislaron un total de 129 cepas de HE de hojas y tallos de ramas jóvenes y maduras de vid, que correspondieron a siete géneros (Trichoderma, Aspergillus, Alternaria, Cladosporium, Fusarium, Nigrospora y Stemphylium). La mayor frecuencia se presentó en las hojas y rama madura. Trichoderma y Alternaria fueron los géneros más frecuentes en las plantas de vid con 22,48 % y 21,71 %, respectivamente. Los metabolitos obtenidos de las cepas HEVH-01 del género Cladosporium, HEV-99 y HEV-70 del género Alternaria, tuvieron mayor efecto en la inhibición micelial del patógeno. Conclusiones. Cepas de hongos endofitos del género Cladosporium y Alternaria mostraron capacidad de antibiosis hacia Botrytis cinerea a escala de laboratorio, los cuales podrían ser potenciales biocontroladores de patógenos en el cultivo de vid.

Abstract This study aimed to craft an extruded product resembling conventional French fries, utilizing rice as the primary ingredient, and incorporating potato blends to enhance physicochemical and sensory attributes during frying. Mashed potatoes, rice flour, and additives were processed using a twin-screw extruder, followed by frying at 160 °C for 2.15 minutes in three stages. In stage I, four formulations were assessed, with the formulation comprising 70% mashed potato, 30% rice flour, and 0.1% salt displaying superior acceptability and minimal oil absorption (8%). Stage II compared vacuum application to non-vacuum conditions, revealing enhanced sensory acceptability with vacuum due to improved texture and firmness. Combining optimal conditions, stage III yielded the best formulation of 70% mashed potatoes, 30% rice flour, 0.1% salt, and 48% moisture. Extrusion conditions result in heightened sensory evaluation across sensorial parameters. This study pioneers a novel avenue for producing healthier rice-based French fry alternatives via extrusion technology, ameliorating frying attributes.

Resumen Este estudio tuvo como objetivo crear un producto extrusionado similar a las papas fritas convencionales, utilizando el arroz como ingrediente principal e incorporando mezclas de papa para mejorar las propiedades fisicoquímicas y sensoriales durante la fritura. Se procesaron papas trituradas, harina de arroz y aditivos mediante una extrusora de doble tornillo, seguido de una fritura a 160 °C durante 2,15 minutos en tres etapas. En la etapa I, se evaluaron cuatro formulaciones, siendo la que contenía 70% de papa triturada, 30% de harina de arroz y 0,1% de sal, la que presentó una aceptabilidad superior y una absorción mínima de aceite (8%). La etapa II comparó la aplicación de vacío con condiciones sin vacío, revelando una mayor aceptabilidad sensorial con vacío debido a una textura y firmeza mejoradas. La combinación de condiciones óptimas en la etapa III produjo la mejor formulación con un 70% de papas trituradas, 30% de harina de arroz, 0,1% de sal y 48% de humedad. Las condiciones de extrusión resultaron en una evaluación sensorial mejorada en parámetros sensoriales. Este estudio pionero abre una nueva vía para producir alternativas más saludables de papas fritas a base de arroz mediante tecnología de extrusión, mejorando las propiedades de fritura.

Resumo Este estudo teve como objetivo criar um produto extrudado similar às batatas fritas convencionais, utilizando o arroz como ingrediente principal e incorporando mixturas de batata para melhorar as propiedades fisico quimicas e sensoriais durante a fritura. Processaram-se batatas trituradas, farinha de arroz e aditivos mediante uma extrusora de dobre parafuso, seguido de uma fritura de 160 °C durante 2,15 minutos en três fases. Na fase I, avaliaram-se quatro formulações, sendo a que tinha 70% de batata triturada, 30% de farinha de arroz e 0,1% de sal, a que apresentou uma aceitabilidade superior e uma absorção mínima de óleo (8%). A fase II comparou a aplicação de vazio com condições sem vazio, revelando uma maior aceitabilidade sensorial com vazio devido a uma textura e firmeza melhoradas. A combinação de condições ótimas na fase III produziu a melhor formulação com um 70% de batatas trituradas, 30% de farinha de arroz, 0,1% de sal e 48% de humidade. As condições de extrusão resultaron numa avaluação sensorial melhorada em parâmetros sensoriais. Este estudo pioneiro abre uma nova via para produzir alternativas mais saudáveis de batatas fritas a base de arroz mediante tecnologia de extrusão, melhorando as propriedades de fritura.

ABSTRACT Potato (Solanum tuberosum L.) seed production using the aeroponic system has been widely implemented in Indonesia. However, aeroponic systems in tropical areas such as Indonesia faced obstacles in the form of high wilt attacks caused by the bacteria Ralstonia solanacearum. This research aimed to control R. solanacearum wilt disease in an aeroponic system using various microbes. The research was carried out from September 2022 to January 2023. This research consisted of four stages, namely exploration and isolation of microorganisms, identification of microbial antagonism, testing of in vitro potential microbial antagonism and testing of selected microbial antagonism in the aeroponic system. The nutrients used in the aeroponic system contained R. solanacearum with a concentration of 104 CFU mL-1, while the concentration of the microbial solution used was 108 CFU mL-1. Pseudomonas fluorescence was consistently the best microbe both in vitro and in aeroponics, whereas Bacillus subtilis could only overcome wilt in vitro and could not overcome wilt in aeroponic system. Pseudomonas fluorescence required 5 min soaking time and could increase wilt with increasing soaking time. The consortium between B. subtilis and P. fluorescence with 10 min soaking time could overcome wilt by 85% and increase the number of tubers and tuber weight by 79% and 85%, respectively, so this prospective microbial consortium could be applied to aeroponic systems environmentally friendly in tropical areas with high R. solanacearum opportunities.

ABSTRACT: Seventy-five pectinolytic strains collected from vegetables grown in the counties of Santarém, Belterra, and Mojuí dos Campos, located in the western region of the Pará State, Brazil, were studied according to their pathological and genetic variability. The strains were grouped in 5 clusters according to pathogenicity in potato, pepper, carrot, and onion, and 38 strains were selected for genetic analysis using rep-PCR. These strains were divided into 35 genetic groups according to rep-PCR at 70% similarity. These results indicated high pathological and genetic variability of the strains causing soft rot in vegetables in the western region of the Pará State, which will be used in etiological research and for the development and assessment of management techniques for the soft rot in vegetables in this region. ABSTRACT Seventyfive Seventy five Santarém Belterra Campos State Brazil potato pepper carrot onion 3 repPCR. repPCR rep PCR. PCR 70 similarity 7

RESUMO: Setenta e cinco isolados de bactérias pectinolíticas coletados de hortaliças cultivadas nos municípios de Santarém, Belterra e Mojuí dos Campos, região oeste do Estado do Pará, Brasil, foram estudadas de acordo com sua variabilidade patológica e genética. Os isolados foram agrupados em cinco grupos de acordo com a patogenicidade em batata, pimenta, cenoura e cebola, e 38 isolados foram selecionados para análise genética por rep-PCR. Esses isolados foram divididos em 35 grupos genéticos de acordo com rep-PCR a 70% de similaridade. Esses resultados indicaram alta variabilidade patológica e genética dos isolados causadores da podridão mole em hortaliças da região oeste do Pará, os quais serão utilizados em pesquisas etiológicas e para o desenvolvimento e avaliação de técnicas de manejo da podridão mole em hortaliças nesta região. RESUMO Santarém Campos Pará Brasil batata pimenta cebola 3 repPCR. repPCR rep PCR. PCR 70 similaridade 7

Abstract In many agricultural crops, arbuscular mycorrhizal fungi co-exist and interact with saprophytic fungi. This study aimed to analyze the effects of two free-living phosphofungi on the growth of potatoes co-inoculated with Claroideoglomus claroideum. Micropropagated native potato plantlets were transferred to a sterile Andisol previously inoculated with C. claroideum and subsequently two saprophytic fungi. The treatments were: C. claroideum (T0), C. claroideum + Talaromyces pinophilus (T1), C. claroideum + Penicillium albidum (T2), and C. claroideum + T. pinophilus + P. albidum (T3). At harvest, the most relevant results show that in T3 the height, shoot dry weight, root length and P acquisition of the potato plants were increased. Therefore, dry weight and P absorbed by minitubers were enhanced two and three-fold, respectively, compared with T0, along with an increase in the length of the thinnest roots. It is concluded that co-inoculation of this fungal consortium could be an advantageous alternative to be used as a bioinoculant by potato tuber seed producers in sustainable agriculture.

Resumen En muchos cultivos agrícolas, los hongos micorrícicos arbusculares coexisten e interactúan con hongos saprófitos. El objetivo de este estudio fue analizar los efectos de dos fosfohongos de vida silvestre sobre el crecimiento de papas co-inoculadas con Claroideoglomus claroideum. Se trasfirieron plántulas de papa nativa micropropagadas a suelo Andisol estéril previamente inoculado con C. claroideum, y subsecuentemente con dos hongos saprófitos. Los tratamientos fueron: C. claroideum (T0), C. claroideum + Talaromyces pinophilus (T1), C. claroideum + Penicillium albidum (T2) y C. claroideum + T. pinophilus + P. albidum (T3). En la cosecha, los resultados más relevantes mostraron que el T3 incrementó la altura, el peso seco de los brotes, la longitud de las raíces y la absorción de P en las plántulas de papa. El peso seco y el P absorbido por los minitubérculos incrementaron al doble y triple, respectivamente, en comparación con el T0; además, aumentó la longitud de las raíces más delgadas. Se concluye que la co-inoculación de dicha asociación de hongos podría ser una alternativa favorable para su uso como bioinoculante por productores de semillas de tubérculos de papa en la agricultura sustentable.

Abstract The objective of this study is to determine the influence of the addition of calcined potato peel on the physical and mechanical properties of hot asphalt mixes - Huánuco, 2023. Regarding the methodology, a quantitative, applied and experimental design study is used, applying the observation and the recording sheet in a sample of 45 briquettes with additions of 2.0 %, 4.0 %, 6.0 % and 8.0 % of calcined potato peel, compared with a standard sample containing 2.0 % hydrated lime, where They evaluate properties such as void content, flow resistance and stability. The results show that the best air content was 3.3 % and flow resistance was 3.4 mm, which was achieved with 2.0 % addition of calcined potato peel; However, the highest stability was obtained with 4.0 % addition, the value being 1,272.3 kg. It is concluded that, with an addition of 2.0 %, the best physical and mechanical properties of the asphalt mixtures are achieved; However, no significant variations were evident in the ANOVA test for flow resistance and stability (p > 0.05).

Resumen El presente estudio tiene como objetivo determinar la influencia de la adición de cáscara de papa calcinada en las propiedades físicas y mecánicas de mezclas asfálticas en caliente - Huánuco, 2023. Con respecto a la metodología, se emplea un estudio cuantitativo, aplicado y de diseño experimental, aplicándose la observación y la ficha de registro en una muestra de 45 briquetas con adiciones de 2.0 %, 4.0 %, 6.0 % y 8.0 % de cáscara de papa calcinada, comparada con una muestra patrón que contiene 2.0 % de cal hidratada, donde se evalúan propiedades como el contenido de vacío, la resistencia al flujo y la estabilidad. Los resultados muestran que el mejor contenido de aire se alcanzó con el 2.0 % de adición, con un valor de 3.3% y la resistencia al flujo fue de 3.4 mm que se alcanzó con 2.0 % de adición de cáscara de papa calcinada; no obstante, la estabilidad más elevada se obtuvo con 4.0 % de adición, siendo el valor de 1,272.3 kg. Se concluye que, con una adición de 2.0 %, se logran las mejores propiedades físicas y mecánicas de las mezclas asfálticas; sin embargo, no se evidenciaron variaciones significativas en la prueba de ANOVA para resistencia al flujo y la estabilidad (p > 0.05).

ABSTRACT Agroindustrial potato (Solanum tuberosum L.) waste mainly includes peel and pulp; however, potatoes of low industrial quality are also discarded by industry. A viable alternative to give added value to these byproducts is the use of these as seeds, as well as the use of starch. The objective of this work was to evaluate the physiological quality (germination and vigor), starch content and relationship between these variables in discarded tubers from the industry through chemical-structural analyses to better understand their influence on starch composition. The physiological quality of the potatoes was evaluated through germination and vigor tests; likewise, starch was extracted, and the yield was determined. Physicochemical characterization of the starches was carried out through proximal and structural analysis using nuclear magnetic resonance (NMR). The results showed that the tubers had 100.00% germination and 98.66% vigor and were suitable for use as seeds. The starch yield did not significantly differ. However, characterization of the starch revealed significant differences (p ≤ 0.05) in moisture, ash, carbohydrate and amylose contents, which were influenced by the storage temperature (4 °C) of the tubers subjected to a vigor test, which interferes with enzymatic activity. During germination, the NMR results showed type B crystallinity, which is typical of tubers, indicating that the viability tests did not promote changes in the type of crystallinity. The results obtained can help us understand the effect of germination on the composition of starch and, accordingly, choose the most appropriate applications for this starch, whether food or nonfood.

Abstract In the pre-Columbian societies of the Americas, a wide range of food practices was observed. However, several countries shared certain staple foods, such as the potato, which has held a significant place in the diet since ancient times. It is important to note that potatoes contain glycoalkaloids, a class of alkaloids with potential toxicity risks when consumed in high concentrations by both humans and animals. This study aims to offer guidance on the presence of glycoalkaloids in potatoes and proposes further research into these compounds in archaeological remains that were utilized as food across all American countries. This recommendation stems from the dearth of studies on this subject, particularly in cases where archaeological discoveries include potato starch granules. In this study, various databases were examined to discover historical insights into the potato and to elucidate the primary aspects of glycoalkaloid chemistry associated with this tuber. The findings underscore the crucial role played by the potato in pre-Columbian cultures of the Americas, particularly in the regions situated along the Andes mountain range. Furthermore, the analysis of its nutritional composition unveiled the prevalence of two key glycoalkaloids in the tuber: α-solanine and α-chaconine. In this research, a comprehensive review of the chemical properties, botanical functions, human metabolism, potential health effects, toxicity thresholds, and available analytical techniques for the detection and quantification of toxic compounds was conducted. The significance of identifying these molecules in archaeological contexts was highlighted, as their presence may prompt investigations into prevalent diseases among historical populations.

Resumen En todo el continente americano, las sociedades precolombinas mostraron prácticas alimentarias diversas; no obstante, diferentes países compartían alimentos comunes. Un ejemplo es la papa, un tubérculo que ha sido un alimento básico en la dieta desde la antigüedad. Las papas contienen glicoalcaloides, una clase de alcaloides que, cuando se ingieren en concentraciones elevadas, pueden plantear riesgos de toxicidad tanto para los seres humanos como para los animales. El objetivo de este estudio es ofrecer una guía sobre glicoalcaloides presentes en papas, y recomendar que se realice investigación de estos compuestos en materiales arqueológicos que fueron destinados para alimentos en todos los países de América. Esta sugerencia surge de la ausencia de estudios de esta naturaleza, particularmente cuando los hallazgos arqueológicos incluyen gránulos de almidón de papa. Se revisaron varias bases de datos para encontrar información histórica sobre la papa e indicar los principales aspectos de la química de los glicoalcaloides relacionados con el tubérculo. Los principales hallazgos indican la importancia que representó la papa en las culturas precolombinas de América, especialmente en las regiones situadas a lo largo de la cordillera de los Andes. La investigación de la composición nutricional reveló que en el tubérculo se pueden encontrar dos glicoalcaloides predominantes: α-solanina y α-chaconina. Se realizó un análisis bibliográfico de estos compuestos tóxicos para comprender su importancia, propiedades químicas, funciones botánicas, metabolismo en el ser humano junto con los posibles problemas de salud, umbrales de toxicidad y las diversas técnicas analíticas disponibles para su detección y cuantificación. La identificación de estas moléculas adquiere importancia en contextos arqueológicos, ya que su presencia puede dar lugar a investigaciones sobre posibles enfermedades prevalentes en la población de la época.

Pesticide residues and heavy metal content of cassava, yam, cocoyam, potato, water yam and carrot were evaluated by gas chromatography-mass spectrometry and atomic absorption spectroscopy. The detected pesticide residues in the samples were 2,4-dichlorophenoxyacetic acid, glyphosate, hexachlorobenzene (HCB), dichlorobiphenyl, aldrin, endosulfan, profenofos, g-chlordane, carbofuran, biphenyl, heptachlor, lindane and t-Nonachlor. The concentration of HCB ranged between 0.0799 ± 0.06 mg/kg and 0.1596 ± 0.00 mg/kg, which was greater than the permitted maximum limit of 0.5 mg/kg established by the US Environmental Protection Agency. The concentration of aldrin and profenofos detected was lower than the predetermined maximum allowed limits. Endosulfan concentrations in cocoyam (0.2500 mg/kg) and potato (0.3265 mg/kg) were higher than the limits allowed by the Canadian Department of Industrial Research. The heavy metals detected in these samples include cobalt, nickel, lead, manganese, chromium, arsenic and mercury in at least one of the samples evaluated. There was not much difference between the concentration of cobalt in yam (0.036 mg/kg) and the maximum allowed concentration (0.043 mg/kg). Lead was detected in potatoes and carrots but was below detectable concentration in cassava, yam, cocoyam and water yam. Similarly, cocoyam was found to have a significant mercury content (0.658 mg/kg), but mercury content was below detectable concentrations in cassava, yam and water yam. SIGNIFICANCE: Heavy metal pollutants and pesticide residues can impair human health, and their presence in food can cause various illnesses and health issues. It is important to prevent exposure to these contaminants and ensure that food is safe by identifying and monitoring them. Farmers may provide consumers with more assurance that their products are safe by identifying and monitoring pesticide residues and heavy metal contamination in these food crops. Overall, it is crucial to find and monitor pesticide residues and heavy metal contamination in food to safeguard customer confidence, ensure legal compliance and preserve human health.

ABSTRACT: Several over-the-counter drugs are offered to patients, and they do not have information on the side effects due to their frequent consumption. This stimulated us to evaluate the antitoxicological effect of potato tocosh (Solanum tuberosum) compared to sulfamethoxasol-trimethopin in onion roots (Allium cepa). Rootlets of 3.0 cm were developed in 20 onion bulbs, which were sustained in four procedures: control (100 ml of distilled water), problem 1 (20 uL of tocosh), problem 2 (sulfamethoxasol-trimethoxasol 1%) and problem 3 (sulfamethoxasol-trimethopine 1% plus 20 uL of tocosh.). The Tjio and Levan staining technique was applied for cellular observation. Group 3 presented: clastogenic chromosomal anomalies: bridges (2.3%), multiple bridges (1.2%), chromosome in c-metaphase (0.5%) and aneugenic: sticky chromosome (1.1%) and yolk nuclear (1.3%) and without aberrations (99.6%) while the group that received antibiotic plus tocosh expressed a lack of chromosomal abnormalities. Under laboratory conditions, at a dose of 2uL of tocosh, the best antitoxicological effect was perceived in roots of Allium cepa which shows tocosh as an antitoxicological alternative to the toxic power of certain drugs.

RESUMEN: Varios fármacos de venta libre son ofrecidos a pacientes y no les alcanzan información de los efectos secundarios por su consumo frecuente. Ello estimuló a valorar el efecto antitoxicológico del tocosh de papa (Solanum tuberosum) frente al sulfametoxasol-trimetropina en raíces de cebolla (Allium cepa). Se desarrollaron raícillas de 3,0 cm en 20 bulbos de cebolla, las cuales se sostuvieron en cuatro procedimientos: testigo (100 ml de agua destilada), problema 1 (20 uL de tocosh), problema 2 (sulfametoxasol-trimetropina 1%) y problema 3 (sulfametoxasol-trimetropina 1% más 20 uL de tocosh.). Se aplicó la técnica de coloración de Tjio y Levan para la observación celular. El grupo 3 presentó: anomalías cromosómicas clastogénicas: puentes (2,3%), puentes múltiples (1,2%), cromosoma en c-metafase (0,5%) y aneugénicas: cromosoma pegajoso (1,1%) y yema nuclear (1,3%) y sin aberraciones (99,6%) mientras la agrupación que recibió antibiótico más tocosh expresó falta de anormalidades cromosómicas. En las condiciones de laboratorio, a una dosis de 2uL de tocosh se percibió el mejor efecto antitoxicológico en raicillas de Allium cepa lo cual muestra al tocosh como una alternativa antitoxicológica ante el poder tóxico de ciertos fármacos.