Resumo Apenas na última década, a obra do engenheiro Uruguaio Eladio Dieste (1917-2000) vem ganhando disseminação acadêmica internacional, sendo que uma de suas principais contribuições para a concepção formal-estrutural na arquitetura e engenharia civil foi o desenvolvimento iterativo da tipologia estrutural das abóbadas gaussianas, cascas de dupla-curvatura estruturadas em cerâmica armada. Entretanto, não há registros de uma definição estabelecida para a geometria arquitetônica destas tipologias estruturais. Tampouco existem evidências concretas acerca da concepção e desenvolvimento projetual destas estruturas. Por conseguinte, o objetivo deste artigo é evidenciar as relações geométricas subjacentes nas abóbadas guassianas descontínuas projetadas e construídas por Eladio Dieste em cerâmica armada, a variação dominante desta tipologia estrutural. Esta análise foi conduzida através da modelagem paramétrica e da regressão polinomial de estudos de caso selecionados a partir de fontes primárias originais, justamente por estas ferramentas computacionais permitirem o correto manejo de geometrias tidas como formalmente complexas. A contribuição esperada para esta pesquisa reside em aprofundar o entendimento entre a relação do processo de projeto e a geometria resultante no objeto de estudo, assim como o entendimento da conformação geométrica desta tipologia estrutural desenvolvida por Eladio Dieste.

Abstract Only within the last decade, the works of Uruguay an Engineer Eladio Dieste (1917-2000) have been gaining international academic dissemination, one of whose main contributions in the realm of formal-structural conception within architecture and civil engineering reside in the iterative development of the structural typology of the gaussian vault shells in reinforced brickwork. However, there is not an established definition for this typology’s architectural geometry. Also, there is hardly any evidence of how such shells were designed. Therefore, the main objective, of this paper is to demonstrate the underlying geometric relationships in the discontinuous gaussian vault shells designed and built by Eladio Dieste in reinforced brickwork, the dominant typological variation within the object of study. This analysis was carried out by means of the parametric modeling and the polynomial regression of selected case studies from the primary sources, since such computational tools allow for the proper geometrical management of formally complex geometries. The expected contributions of this study reside in the deepening of the understanding of the relationship between the design process and the geometry of the object of study, as well as the understanding of the geometrical conformation of the structural typology conceived by Eladio Dieste.

Abstract The methodology for mixture design can be used to optimize the proportions of ions in nutrient solution, thereby maximizing crop yield; however, it is rarely used. The aim of this work was to apply a mixture design to evaluate nutrient solutions with different proportions of K+, Ca2+ and Mg2+ on the average weight, number and yield of fruits, and to determine the optimal combination that maximizes the fruit yield of tomato (Solanum lycopersicum cv. Legionario) grown in a closed hydroponic system. The simplex-lattice experimental design {3,2} was used, with 10 nutrient solutions and a total concentration of 40 meq∙L-1 of ions in each solution. The maximum values of fruit number and yield (27 and 135.8 t∙ha-1, respectively) were obtained with proportions of 0.375 K+, 0.400 Ca2+ and 0.225 Mg2+ in the nutrient solution, equivalent to 7.5, 8 and 4.5 meq∙L-1, respectively. The polynomial regression model estimated to predict yield as a function of K+, Ca2+ and Mg2+ mixtures is of the fourth degree (special quartic), with a coefficient of determination (R2) equal to 0.81, which explains 81 % of the variability of the yield data. The maximum estimated yield was 142 t∙ha-1, with partial desirability of 0.964, which would be obtained with 0.358 K+, 0.421 Ca2+ and 0.220 Mg2+, equivalent to 7.28, 8.26 and 4.46 meq∙L-1, respectively, in the nutrient solution.

Resumen La metodología para el diseño de mezclas se puede emplear para optimizar las proporciones de los iones en solución nutritiva, y con ello maximizar el rendimiento de los cultivos; sin embargo, su uso es escaso. El objetivo del presente trabajo fue aplicar un diseño de mezclas para evaluar soluciones nutritivas con diferentes proporciones de K+, Ca2+ y Mg2+ sobre el peso medio, número y rendimiento de frutos, y determinar la combinación óptima que maximice el rendimiento de fruto de tomate (Solanum lycopersicum cv. Legionario) cultivado en sistema hidropónico cerrado. Se utilizó el diseño experimental látice simplex {3,2}, con 10 soluciones nutritivas y una concentración total de 40 meq∙L-1 de iones en cada solución. Los valores máximos de número y rendimiento de frutos (27 y 135.8 t∙ha-1, respectivamente) se obtuvieron con proporciones de 0.375 K+, 0.400 Ca2+ y 0.225 Mg2+ en la solución nutritiva, equivalentes a 7.5, 8 y 4.5 meq∙L-1, respectivamente. El modelo de regresión polinomial estimado para predecir el rendimiento en función de las mezclas de K+, Ca2+ y Mg2+ es de grado cuatro (cuártico especial), con coeficiente de determinación (R2) igual a 0.81, el cual explica en 81 % la variabilidad de los datos de rendimiento. El máximo rendimiento estimado fue de 142 t∙ha-1, con deseabilidad parcial de 0.964, que se obtendría con 0.358 K+, 0.421 Ca2+ y 0.220 Mg2+, equivalentes a 7.28, 8.26 y 4.46 meq∙L-1, respectivamente, en la solución nutritiva.

RESUMO: A Eryngium foetidum apresenta desuniformidade e baixas taxas de germinação. Assim, visando reduzir as problemáticas durante a produção de mudas, alguns tratamentos podem ser realizados. Por isso, essa pesquisa teve como objetivo avaliar a qualidade fisiológica de sementes de chicória da Amazônia submetidas a tratamentos de imersão em água. Foram realizados dois experimentos, em condição controlada de temperatura (25 ºC) e em condição de viveiro, testados seis tratamentos (0, 12, 24, 36 e 48 horas) em ambos. Na condição controlada, utilizou-se delineamento inteiramente casualizado e no viveiro, blocos ao acaso. Sendo avaliado: índice de velocidade de germinação/emergência (IVG/IVE), tempo médio de germinação/emergência (TMG/TME), porcentagem de germinação/emergência, desenvolvimento de plântulas normais, massa fresca e massa seca. Os dados foram submetidos à análise de regressão linear e polinomial de 2ª ordem no programa estatístico computacional “R”. Na condição controlada, o IVG apresentou valor máximo de 4,65 após estimativa de 18,03 horas de imersão, para o TMG 12 h de imersão proporcionou tempo médio menor (13,76 dias) e obteve 39,26% de sementes germinadas após estimativa de 26,27 h de imersão, assim como 19,34% de plântulas normais em 27,97 h. Enquanto no viveiro, para o TME observou menor valor após 48 h e massa fresca e seca se obteve maiores médias após 12 h, 18,47 g e 1,44 g, respectivamente. Assim, a imersão em água por 12 horas proporcionou menor tempo de germinação e maior massa fresca e seca, possivelmente o tratamento auxiliou na reativação do metabolismo e crescimento do embrião.

ABSTRACT: Eryngiumfoetidum exhibits low germination rates and heterogeneous seed maturation. Thus, in order to reduce problems during seedling production, a number of treatments can be used. The aim of this study was to assess the physiological quality of Amazon chicory seeds submitted to water immersion treatments. Two experiments were conducted, under controlled temperature (25 ºC) and nursery conditions, with six treatments (0, 12, 24, 36 and 48 hours) in each. A completely randomized design was used in the controlled condition and random blocks in the nursery. The following were assessed: germination/emergence speed index (GSI/ESI), mean germination/emergence time (MGT/MET), germination/emergence percentage, normal seedling development, fresh weight and dry weight. The data were submitted to linear and second-order polynomial regression analysis in the R statistical program. Under controlled conditions, Maximum GSI was 4.65 after 18.03 hours of immersion, and for MGT, 12 h of immersion provided a shorter average time (13.76 days) and obtained 39.26% of germinated seeds after 26.27 h of immersion, as well as 19.34% of normal seedlings in 27.97 h. In the nursery, a lower MET was observed after 48 h and mean fresh and dry weight were higher after 12 h (18.47 and 1.44 g, respectively). Thus, water immersion for 12 hours resulted in a shorter germination time and higher fresh and dry weight, and the treatment may have helped reactivate embryo metabolism and growth.

RESUMO Em sistemas de irrigação por gotejamento subsuperficial, o conhecimento do avanço tridimensional da água no solo é essencial na seleção do espaçamento e profundidade de instalação dos emissores. O objetivo desta pesquisa foi desenvolver e testar diferentes modelos matemáticos para estimar a distribuição de água no solo sob irrigação por gotejamento subsuperficial. O experimento foi montado em delineamento inteiramente casualizado com quatro repetições. O arranjo experimental foi de parcelas subdivididas no tempo, tendo nas parcelas diferentes profundidades de instalação de gotejadoras (0, 5, 10, 15, 20, 25 e 30 cm) e nas subparcelas tempos de aplicação da irrigação (0, 60, 120, 180 e 240 min). Foram construídos três modelos (SLIDE 6.0, regressão polinomial e SPSS 2.0) para estimar a distribuição de água no perfil do solo. Todos os modelos apresentaram índices estatísticos dentro de faixas aceitáveis. Na comparação, o modelo gerado no software numérico SLIDE 6.0 foi o que apresentou melhor desempenho, seguido do SPSS 2.0 e regressão polinomial. Os modelos gerados foram eficientes e simples, apresentando bons resultados na predição da distribuição de água no perfil do solo para as condições estudadas.

ABSTRACT In subsurface drip irrigation systems, knowledge of the three-dimensional advancement of water in the soil is essential for selecting emitter spacing and installation depth. This research aimed to develop and test different mathematical models to estimate water distribution in the soil under subsurface drip irrigation. The experiment was set up in a completely randomized design with four replicates. The experimental arrangement was of split-plot in time, with different dripper installation depths in the plots (0, 5, 10, 15, 20, 25, and 30 cm) and in the subplots irrigation application times (0, 60, 120, 180, and 240 min). Three models (SLIDE 6.0, polynomial regression, and SPSS 2.0) were constructed to estimate the water distribution in the soil profile. All models showed statistical indexes within acceptable ranges. In comparison, the model generated in the numerical software SLIDE 6.0 was the one that presented the best performance, followed by SPSS 2.0 and polynomial regression. The generated models were efficient and simple, producing good results in predicting the water distribution in the soil profile under the studied conditions.

RESUMEN Antecedentes: El nivel de carga calórica acumulada del ITH (CCA) durante los 6 meses previos al destete sobre el peso al destete (PD), fue evaluado en animales Charoláis como expresión de stress térmico (ST), así como su posible componente genético. Métodos: Se analizaron 19 466 registros de PD de la Empresa Manuel Fajardo, Jiguaní, Granma, nacidos entre 1988 y 2017 en 16 rebaños. Se utilizó un modelo de Norma de Reacción considerando efecto fijo cuatrimestres-años-rebaños (859 clases), sexo del ternero (2) y número de partos de la madre (11), una covariable fija mediante un polinomio de Legendre (L) de orden 3 para representar la curva de respuesta del PD en la trayectoria de CCA. Como aleatorios: residuo y la desviación del animal que produce el registro que se modeló por un L de orden 1. Con los valores genéticos estandarizados (VG) se hizo un análisis de componentes principales y con los coeficientes de los eigenvector se construyó un índice de los resultados. Resultados: El análisis de regresión arrojó que el PD se deprime en b=-0,807±0,04 kg por unidad de CCA (R2=90,2%). Se diferenciaron tres zonas: fría CCA<=439; neutra CCA>=440' a <=450 y caliente CCA >=451, con PD= 149,3; 140,5 y 134,1 kg respectivamente. La variación en VG es mayor en la zona fría, pero las correlaciones fueron muy bajas (0,33) evidenciando interacción genotipo ambiente (Ige). Conclusiones: Se demostró existencia de una base genética para tolerancia a ST con manifestaciones de Ige, y cambia el orden de mérito en condiciones extremas.

ABSTRACT Background: The level of accumulated caloric load of the ITH (CCA) during the 6 months prior to weaning on the weaning weight (PD) was evaluated in Charolais animals as an expression of thermal stress (ST), as well as its possible genetic component. Methods: 19,466 PD records from the Manuel Fajardo Enterprise, Jiguaní, Granma, born between 1988 and 2017 in 16 herds, were analyzed. A Reaction Norm model was used considering a fixed effect of quarters-years-herds (859 classes), sex of the calf (2) and number of calving of the mother (11), a fixed covariate using a Legendre polynomial (L) of order 3 to represent the PD response curve in the CCA trajectory. As random: residual and the deviation of the animal that produces the record that was modeled by an L of order 1. With the standardized genetic values (VG) a principal components analysis was done and with the eigenvector coefficients an index of the results. Results: The regression analysis showed that PD is depressed by b=-0,807±0,04 kg per unit of CCA (R2=90,2%). 3 zones were differentiated: cold CCA<=439; neutral CCA>=440 to <=450 and hot CCA>=451, with PD= 149,3; 140,5 and 134,1 kg respectively. The variation in VG is greater in the cold zone, but the correlations were very low (0,33), evidencing genotype-environment interaction (Ige). Conclusions: The existence of a genetic basis for tolerance to ST with Ige manifestations was demonstrated, and the order of merit changes in extreme conditions.

Resumo A salinidade é um dos parâmetros ambientais mais críticos em relação à fisiologia dos peixes, modificando a ingestão de alimentos e o desempenho de crescimento em muitas espécies. O presente estudo investigou os efeitos de diferentes níveis de salinidade no desempenho de crescimento, alimentação e sobrevivência de jovens espécies de robalos asiáticos Lates calcarifer, as quais foram criadas sob salinidade 0 (T1), 5 (T2), 22 (T3), 36 (T4) e 42 (T5) ppt. Aproximadamente 830 indivíduos de peixes com peso médio de 1,24 ± 0,52 g foram distribuídos aleatoriamente (166 peixes / tanque) em cinco tanques de concreto (cada tanque tinha 30 × 6 × 4 pés (9,1 x 1,8 x 1,2 metros), com volume de 19,12 litros) por 40 dias. Os peixes foram inicialmente alimentados com uma dieta contendo 42% de proteína bruta a uma taxa de 6% do seu peso corporal por dia. Os resultados mostraram que o nível de salinidade teve um efeito significativo no ganho de peso (GP), ganho de peso médio diário (GPMD), taxa de crescimento específico (TCE), taxa de conversão alimentar (TCA), taxa de sobrevivência (TS), biomassa total e índices de saúde (p < 0,05). O maior GP (39,11 ± 1,49 g), GPMD (1,00 ± 0,12 g), TCE (8,74 ± 0,03% d-1) e o menor TCA (0,96 ± 0,20) foram observados com o tratamento T3, que foi significativamente superior em comparação com os outros tratamentos (p < 0,05). Entre os índices de saúde, os maiores índices hepatossomáticos e viscerossomáticos foram encontrados no tratamento T3, significativamente superior do que os demais grupos (p < 0,05). Não foram encontradas diferenças significativas entre os tratamentos quanto à TS (p > 0,05), mas a TS máxima (98,89 ± 0,0%) foi observada nos tratamentos T3 e T2. O nível máximo de proteína bruta (19,99 ± 1,4%) foi encontrado na composição bioquímica dos corpos dos jovens robalos asiáticos no grupo T3. A regressão polinomial de segunda ordem mostrou que a salinidade de 20 ppt é ótima para o melhor crescimento do robalo asiático. Assim, o presente estudo recomenda salinidade de 20 a 36 ppt para o cultivo comercial de robalo asiático em sistema de aquicultura fechado.

Abstract Salinity is one of the most critical environmental parameters regarding fish physiology, modifying food intake and growth performance in many fish species. The present study has investigated the effects of different salinity levels on growth performance, feeding and survival of Asian seabass Lates calcarifer juveniles. Asian seabass juveniles were reared at 0 (T1), 5 (T2), 22 (T3), 36 (T4), and 42 (T5) ppt salinity. Approximately eight hundred thirty fish individuals with an average weight of 1.24±0.52 g were randomly distributed (166 fish/Tank) in 5 concrete tanks (each tank 30×6×4 ft, volume 19,122 L) for forty days. Juveniles were initially fed 42% crude protein-containing diets at a rate of 6% of their body weight per day. The results showed that salinity level had a significant effect on the weight gain (WG), average daily weight gain (ADWG), specific growth rate (SGR), feed conversion ratio (FCR), survival rate (SR), total biomass and health indices (p<0.05). The highest WG (39.11±1.49 g), ADWG (1.00±0.12 g), SGR (8.74±0.03% d-1) and lowest FCR (0.96±0.20) were observed with T3 treatment, which was significantly higher compared to other treatment groups (p<0.05). Among the health indices, the highest hepatosomatic index and viscerosomatic index were found with T3 treatment, significantly higher than the other groups (p<0.05). No significant differences were found among the treatments in terms of survival rate (p>0.05), but the maximum survival rate (98.89±0.0%) was observed in the T3 and T2 treatments. The maximum level of crude proteins (19.99±1.4%) was found in the whole-body biochemical composition of Asian seabass juveniles in the T3 treatment group. The second-order polynomial regression showed that 20 ppt salinity is optimum for the best growth of Asian seabass. Thus, the present study recommends 20 to 36 ppt salinity for the commercial farming of Asian seabass under a closed aquaculture system.

RESUMO Objetivo: Investigar a associação isolada e combinada entre distúrbios do sono (duração do sono, sintomas de insônia nas últimas 30 noites e cansaço diurno) e desempenho em testes cognitivos. Métodos: Análise transversal dos dados da visita 2 (2012–2014) do Estudo Longitudinal de Saúde do Adulto de coorte de servidores públicos ativos e aposentados de seis capitais brasileiras. Regressão polinomial com termo quadrático e modelos de regressão linear múltipla foram realizados para avaliar associações isoladas e combinadas entre distúrbios do sono e desempenho na memória, fluência, funções executivas e cognição global. Resultados: Foram incluídos um total de 7.248 participantes, com média etária de 62,7 anos (desvio padrão [DP]=5,9), sendo 55,2% mulheres. Associações em forma de U invertido foram observadas entre duração do sono e desempenho em todas as habilidades cognitivas, sugerindo que durações menores ou maiores que sete horas estão associadas ao pior desempenho, independentemente da idade. O relato de insônia foi associado à pior função executiva (β: -0.08; IC95% -0.15 a -0.01), sendo as magnitudes das associações maiores para indivíduos com insônia em dois ou mais momentos (β: -0.12; intervalo de confiança [IC]95% -0.19 a -0.05) ou, especialmente, insônia combinada com sono curto (β: -0.18; IC95% -0.24 a -0.11). Insônia em dois ou mais períodos também foi associada à menor memória e cognição global. Não houve associação entre qualquer distúrbio do sono testado e fluência verbal. Cansaço diurno isolado não foi associado ao desempenho nos testes avaliados. Conclusão: Os resultados sugerem que a duração extrema do sono é prejudicial para quase todas as funções cognitivas investigadas, enquanto a insônia parece afetar mais fortemente a função executiva.

ABSTRACT Objective: To investigate the single and combined associations between sleep disturbances (sleep duration, insomnia symptoms in the last 30 nights, and daytime tiredness) and performance in cognitive tests. Methods: Cross-sectional analysis of data from visit 2 (2012–2014) of the Longitudinal Study of Adult Health from a cohort of active and retired civil servants from six Brazilian capitals. Polynomial regression with quadratic term and multiple linear regression models were performed to assess single and combined associations between sleep disturbances and memory performance, fluency, executive functions, and global cognition. Results: A total of 7,248 participants were included, with a mean age of 62.7 years (standard deviation [SD]=5.9), and 55.2% were women. Inverted U-shaped associations were observed between sleep duration and performance on all cognitive abilities, suggesting that durations shorter or longer than seven hours are associated with worse performance, regardless of age. Reported insomnia was associated with worse executive function (β: -0.08; 95% confidence interval [CI]: -0.15 to -0.01), and the magnitudes of associations were higher for individuals with insomnia at two or more moments (β: -0.12; 95%CI -0.19 to -0.05) or, especially, insomnia combined with short sleep (β: -0.18; 95%CI -0.24 to -0.11). Insomnia in two or more periods was also associated with lower memory and global cognition. There was no association between any sleep disturbance tested and verbal fluency. Isolated daytime tiredness was not associated with performance in the evaluated tests. Conclusion: The results suggest that extreme sleep durations are detrimental to almost all cognitive abilities investigated, whereas insomnia appears to affect more severely the executive function.

ABSTRACT Despite being very common in the territory of Chihuahua, Chihuahua, Mexico, to experience drought, its consequences continue to severely impact the population without prior warning. Machine learning has proven to have a significant capacity for predicting time series, and the Standardized Precipitation Evapotranspiration Index (SPEI) is emerging as the most accurate drought indicator. In this study, predictive models were developed using Artificial Neural Networks (ANN), Long-Short Term Memory (LSTM), and Support Vector Regression (SVR) for estimating SPEI. Temporal scales of 12 months (SPEI 12) and 24 months (SPEI 24) for the period 1901-2020 in the mentioned territory were considered. This was done in order to simulate the behavior of drought cycles and enhance the ability to anticipate consequences. The accuracy indices used to evaluate the models were the mean squared error (MSE), mean absolute error (MAE), mean bias error (MBE), coefficient of determination (R2), and Kendall coefficient. In total, 956 experiments were conducted using the three methods, varying parameters such as the number of neurons, kernel, and polynomial degree. The two best models for each method were selected, and the average results revealed MSE = 0.0051, MAE = 0.0537, MBE = 0.0218, R2 = 0.8495, and Kendall coefficient = 0.7592 for SPEI 12; and MSE = 0.0024, MAE = 0.0375, MBE = 0.0162, R2 = 0.9218, and Kendall coefficient = 0.8558 for SPEI 24.

RESUMEN A pesar de ser muy común que el territorio de Chihuahua, Chihuahua, México, experimente sequía, sus consecuencias continúan impactando severamente a la población sin previo aviso. El aprendizaje automático ha demostrado tener una importante capacidad para predecir series temporales, y el índice estandarizado de evapotranspiración y precipitación (SPEI, por su sigla en inglés) se perfila como el indicador de sequía más preciso. En este estudio, se desarrollaron modelos predictivos utilizando redes neuronales artificiales (ANN), memoria a largo y corto plazo (LSTM) y regresión de vectores de soporte (SVR) para estimar el SPEI. Se consideraron escalas temporales de 12 (SPEI 12) y 24 meses (SPEI 24) para el periodo 1901-2020 en el territorio mencionado. Esto se hizo para simular el comportamiento de los ciclos de sequía y mejorar la capacidad de anticipar las consecuencias. Los índices de precisión utilizados para evaluar los modelos fueron el error cuadrático medio (MSE), el error absoluto medio (MAE), el error de sesgo medio (MBE), el coeficiente de determinación (R2) y el coeficiente de Kendall. En total, se realizaron 956 experimentos con los tres métodos, variando parámetros como el número de neuronas, el kernel y el grado del polinomio, entre otros. Se seleccionaron los dos mejores modelos para cada método y los resultados promedio revelaron MSE = 0.0051, MAE = 0.0537, MBE = 0.0218, R2 = 0.8495 y coeficiente de Kendall = 0.7592 para SPEI 12, y MSE = 0.0024, MAE = 0.0375, MBE = 0.0162, R2 = 0.9218 y coeficiente de Kendall = 0.8558 para SPEI 24.

This study evaluated the external and internal quality characteristics after various storage periods (SP) of eggs from different management practices (MP) regarding layer hen housing and feeding systems. One hundred eggs collected from farms with Lohman Sandy hens raised in i) a cage system where the standard diet was offered ad libitum (CAB), ii) a free-range production system with free access to both the standard diet and alfalfa pasture for grazing (FRG), or iii) a village poultry system where hens were fed on insects, grass, vegetables, or kitchen waste, as well as a restricted standard diet (VCR) were stored at 0, 7, 14, 21, and 28 d at +4 °C and 60% relative humidity. Data were analysed in a three MP χ five SP factorial arrangement with 20 eggs each. SP was analysed using polynomial regression. The CAB eggs had a higher weight loss and shell ratio compared to the FRG and VCR eggs. The yolk index, albumen index, Haugh unit (HU), and yolk colour of FRG and VCR eggs were higher than CAB eggs. The yolk ratio of the CAB group was higher than that of the other groups, whereas the albumen ratio was lower. The egg weight loss increased linearly, whereas the albumen ratio and pH increased cubically during storage. The egg weight loss, shell thickness, and yolk index were related to the interaction between MP and SP. Shell ratio and all internal quality traits, except for the pH of yolk and albumen, reflected only the effect of the MP; the association became more significant as the SP increased. Our results suggest that shell ratio, surface area, and all internal quality traits, except for yolk colour, change during storage.

Carcass characteristics and primal pork cuts of local pig breeds are rarely documented, therefore, the current study was conducted to establish the relation between incremental levels of Amarula oil cake (AOC), carcass traits, primal pork cuts, and relative internal organ weight of Windsnyer pigs. Twenty-five clinically healthy, growing, male Windsnyer pigs with an initial body weight of 19.92 ± 8.74 kg (mean ± standard deviation) were used. Pigs were used in their growing period when they were about 67 days old. The study lasted six weeks excluding one week of adaptation period. Pigs were allotted to pens in a completely randomized design and assigned to each of the five experimental diets, which contained 0, 50, 100, 150 and 200 g/kg DM of Amarula oil cake, respectively. Feed and water were offered ad libitum. Post slaughter, data for carcass characteristics, primal pork cuts, and relative organ weights of Windsnyer pigs were analysed using polynomial regression. There was a negative linear relationship between increasing levels of Amarula oil cake, carcass length, warm carcass weight and cold carcass weight. Stomach weight, backfat thickness, drip loss, and the hepatosomatic index increased linearly with increasing levels of Amarula oil cake. The kidneys, small intestines, and large intestine weight had a quadratic response to Amarula oil cake inclusion level. The heart, lungs, and spleen were not related to increasing levels of Amarula oil cake inclusion. Incremental levels of Amarula oil cake diets impaired carcass characteristics and the selected visceral organs, therefore Windsnyer pigs can be fed Amarula oil cake up to100 grams per kilograms dry matter.

RESUMO Objetivo: Investigar a associação isolada e combinada entre distúrbios do sono (duração do sono, sintomas de insônia nas últimas 30 noites e cansaço diurno) e desempenho em testes cognitivos. Métodos: Análise transversal dos dados da visita 2 (2012–2014) do Estudo Longitudinal de Saúde do Adulto de coorte de servidores públicos ativos e aposentados de seis capitais brasileiras. Regressão polinomial com termo quadrático e modelos de regressão linear múltipla foram realizados para avaliar associações isoladas e combinadas entre distúrbios do sono e desempenho na memória, fluência, funções executivas e cognição global. Resultados: Foram incluídos um total de 7.248 participantes, com média etária de 62,7 anos (desvio padrão [DP]=5,9), sendo 55,2% mulheres. Associações em forma de U invertido foram observadas entre duração do sono e desempenho em todas as habilidades cognitivas, sugerindo que durações menores ou maiores que sete horas estão associadas ao pior desempenho, independentemente da idade. O relato de insônia foi associado à pior função executiva (β: -0.08; IC95% -0.15 a -0.01), sendo as magnitudes das associações maiores para indivíduos com insônia em dois ou mais momentos (β: -0.12; intervalo de confiança [IC]95% -0.19 a -0.05) ou, especialmente, insônia combinada com sono curto (β: -0.18; IC95% -0.24 a -0.11). Insônia em dois ou mais períodos também foi associada à menor memória e cognição global. Não houve associação entre qualquer distúrbio do sono testado e fluência verbal. Cansaço diurno isolado não foi associado ao desempenho nos testes avaliados. Conclusão: Os resultados sugerem que a duração extrema do sono é prejudicial para quase todas as funções cognitivas investigadas, enquanto a insônia parece afetar mais fortemente a função executiva.

ABSTRACT Objective: To investigate the single and combined associations between sleep disturbances (sleep duration, insomnia symptoms in the last 30 nights, and daytime tiredness) and performance in cognitive tests. Methods: Cross-sectional analysis of data from visit 2 (2012–2014) of the Longitudinal Study of Adult Health from a cohort of active and retired civil servants from six Brazilian capitals. Polynomial regression with quadratic term and multiple linear regression models were performed to assess single and combined associations between sleep disturbances and memory performance, fluency, executive functions, and global cognition. Results: A total of 7,248 participants were included, with a mean age of 62.7 years (standard deviation [SD]=5.9), and 55.2% were women. Inverted U-shaped associations were observed between sleep duration and performance on all cognitive abilities, suggesting that durations shorter or longer than seven hours are associated with worse performance, regardless of age. Reported insomnia was associated with worse executive function (β: -0.08; 95% confidence interval [CI]: -0.15 to -0.01), and the magnitudes of associations were higher for individuals with insomnia at two or more moments (β: -0.12; 95%CI -0.19 to -0.05) or, especially, insomnia combined with short sleep (β: -0.18; 95%CI -0.24 to -0.11). Insomnia in two or more periods was also associated with lower memory and global cognition. There was no association between any sleep disturbance tested and verbal fluency. Isolated daytime tiredness was not associated with performance in the evaluated tests. Conclusion: The results suggest that extreme sleep durations are detrimental to almost all cognitive abilities investigated, whereas insomnia appears to affect more severely the executive function.

RESUMO: A concentração de corticosterona plasmática - CORT, relação heterófilo:linfócito - H:L, atividade enzima catalase - CAT, concentração glutationa total - GSH e níveis de substâncias reativas ao ácido tiobarbitúrico - TBARS foram avaliadas em 48 poedeiras Hy Line Brown, 79 semanas de idade, submetidas ao jejum alimentar por 10 dias consecutivos. Coletou-se sangue no dia zero, dois, quatro, seis, oito e 10 do período de jejum alimentar, adotando-se um delineamento inteiramente casualizado, com oito repetições em cada dia de coleta, sendo cada ave uma unidade experimental. Através da análise da regressão polinomial determinou-se o momento de máximo estresse para cada metodologia. Realizou-se análise de correlação de Pearson para determinar se pode haver substituição entre os métodos. A CORT e GSH apresentaram o momento de máximo estresse aos 4,3 dias de jejum alimentar, enquanto que H:L e CAT aos 10 dias. TBARS apresentou maior concentração de malondialdeído no dia zero de jejum alimentar e a menor aos 5,5 dias, não sendo uma metodologia confiável para medir o estresse. Os baixos coeficientes de correlação de Pearson entre as metodologias impossibilitam a indicação de apenas um método em substituição aos demais para mensurar o estresse de poedeiras durante o período de jejum alimentar.

ABSTRACT: Plasma corticosterone concentration (CORT), heterophil:lymphocyte ratio (H:L), catalase activity (CAT), total glutathione concentration (GSH), and thiobarbituric acid reactive substance levels (TBARS) were evaluated in 48 Hy-Line Brown laying hens, at 79 weeks of age, after being fasted for 10 consecutive days. Blood was collected on days zero, two, four, six, eight, and 10 of fasting, and a completely randomized design was adopted, with eight replicates on each day of collection, with each bird being an experimental unit. The time of maximum stress was determined for each method, using the polynomial regression analysis. The Pearson correlation analysis was also performed to determine whether the methods were interchangeable. CORT and GSH concentrations indicated that the time of maximum stress occurred at 4.3 days of fasting, whereas, the H:L and CAT activity indicated that the time of maximum stress occurred at 10 days of fasting. The malondialdehyde concentration detected by the TBARS method was highest at day zero and lowest at day 5.5 of fasting, but this method was not a reliable measure of stress. The low Pearson correlation coefficients observed among the methods made it impossible to designate only one of the tested methods as a replacement for the others, to measure the stress in laying hens during fasting.

ABSTRACT The objective of the current study was to investigate the effects of different levels of andiroba oil (AO) in a bioproduct based on andiroba oil (BBAO) on the physical quality, bacteriological concentration, chemical composition, yolk lipid oxidation, and sensory characteristics when coating eggs and storing them for seven days at room temperature. The eggs were arranged in a completely randomized design, in which treatments consisted of a control group (eggs stored for seven days without the application of BBAO) and eggs covered with biofilms produced with BBAO with different levels of AO (1%, 5%, 10%, and 15%). Each treatment contained 50 eggs, and each egg was considered a replicate. Data collected were subjected to Tukey test and linear or polynomial regression at 0.05 statistical significance. The results indicate that the application of BBAO on eggs stored for 7 days at room temperature resulted in better (p<0.05) conservation of their physical quality and chemical composition, and a reduction (p<0.05) or elimination of bacteriological concentrations. This conservation effect became increasingly pronounced as the concentration of AO in the BBAO increased. However, it is crucial to consider the implications of BBAO on sensory characteristics, as higher concentrations of AO in BBAO lead to a decreased (p<0.05) sensory acceptance of the eggs. Therefore, it was concluded that the use of AO in the bioproduct, especially at high levels, can provide better conservation of the eggs for seven days at room temperature, particularly concerning the physical, chemical, and microbiological characteristics; however, it can also cause significant changes in sensory attributes.

ABSTRACT. This study aimed to assess the impact of incorporating fish waste oil into laying hens' diets on apparent nutrient digestibility and apparent metabolizable energy. A total of 72 Hisex White laying hens (25 weeks old) were employed in a completely randomized design, with treatments consisting of a control diet and an experimental diet (containing 3.5% fish oil), each with six replicates of six birds. The collected data underwent polynomial regression analysis at a 5% significance level. No significant differences (p > 0.05) were observed in the digestibility of dry matter, crude protein, mineral matter, crude fiber, and non-nitrogenous extract between the reference diet and the experimental diet containing fish waste oil. However, there were significant differences (p < 0.05) in the digestibility of ethereal extract (fat). Despite this, values for apparent metabolizable energy and apparent metabolizable energy corrected by nitrogen balance did not exhibit significant differences (p > 0.05) between the reference and experimental diets. In conclusion, the incorporation of 3.5% fish waste oil in laying hens' diets led to satisfactory nutrient digestibility and enhanced fat digestibility. Importantly, this inclusion did not adversely impact energy metabolism.

ABSTRACT The present study was carried out to determine the protein requirement in Peruvian grunt juvenile, Anisotremus scapularis. In total, 72 fish weighing 19.95 ± 4.09 g and length of 10.31 ± 0.63 cm were used. They were randomly distributed in groups of six units per tank. The experimental diets contained protein levels of 45, 50, 55 and 58%, which were supplied to three experimental units per diet. The fish were fed three times a day to satiety for a period of 56 days. The results showed that final weight, weight gain, feed intake and feed conversion ratio were significantly (p<0.05) affected by dietary protein levels. However, the increase in size and the specific growth rate did not present significant differences (p>0.05) between treatments. The protein requirement based on weight gain using second degree polynomial regression was 56.78%, for optimal growth of Peruvian grunt juveniles.

RESUMEN El presente estudio fue realizado para determinar el requerimiento de proteína del alimento en juveniles de Chita, Anisotremus scapularis. Se utilizaron 72 peces con un peso de 19.95 ± 4.09 g y 10.31 ± 0.63 cm de longitud, los cuales fueron distribuidos aleatoriamente en grupos de seis unidades por tanque. Las dietas experimentales contenían niveles de proteína de 45, 50, 55 y 58%, las que fueron suministradas a tres unidades experimentales por dieta. Los peces fueron alimentados tres veces por día hasta la saciedad por un periodo de 56 días. Los resultados mostraron que el peso final, ganancia de peso, consumo de alimento y conversión alimenticia fueron significativamente (p<0.05) afectados por los niveles de proteína en la dieta. Sin embargo, el incremento de longitud y la tasa especifica de crecimiento no presentaron diferencias significativas (p>0.05) entre tratamientos. El requerimiento de proteína determinado en base a la ganancia de peso mediante la regresión polinomial de segundo grado fue de 56.78% para el óptimo crecimiento de juveniles de chita.