ABSTRACT Objective: To describe the evidence of Platelet Rich Plasma (PRP), Stem cells therapy (SCT) and Extracorporeal shockwave therapy (ESWL) for the treatment of Peyronies disease (PD), including information from the main urological society guidelines. Materials and Methods: A literature review of PubMed articles published between 2000 and 2023 was conducted, utilizing keywords such as "Peyronie's Disease", "Penile curvature", "Platelet Rich Plasma", "Stem cells", and "Extracorporeal shockwave therapy". Only full-text articles in English were included, excluding case reports and opinions. Results: A considerable number of clinical trials were conducted using PRP penile injections for therapy of PD, showing reduction of curvature, plaque size and improvement in quality of life. Preclinical studies in rats have shown the potential benefit of adipose-derived stem cells, with improvements in erectile function and fibrosis. Human studies with mesenchymal stem cells demonstrated promising results, with reduction of curvature and plaque size. ESWL effects on PD were investigated in randomized clinical trials and demonstrated no significant impact in curvature or plaque size, but reasonable effect on pain control. Conclusion: Restorative therapies has emerged as an innovative treatment option for PD and the results from current studies appear to be promising and demonstrated good safety profile. Unfortunately, due to scarce evidence, PRP and SCT are still considered experimental by American Urological Association (AUA) and European Association of Urology (EAU) guidelines. ESWT is recommended, by the same guidelines, for pain control only. More high-quality studies with long-term follow-up outcomes are needed to evaluate efficacy and reproducibility of those therapies. Objective PRP, , (PRP) (SCT (ESWL (PD) guidelines Methods 200 202 Peyronie s Disease, Disease Disease" Penile curvature" Plasma, Plasma" cells" therapy. . therapy" fulltext full text included opinions Results life adiposederived adipose derived fibrosis Conclusion profile Unfortunately AUA (AUA EAU (EAU recommended only highquality high longterm long term followup follow up (PRP (PD 20 2

Abstract The aim of the research is to perform an exploratory review showing studies evaluating the use of autologous platelet-rich plasma (PRP) as an additional treatment to hormonal treatment to increase endometrial thickness in patients with infertility related to endometrial thickness. We conducted a literature search since 2015 to 2024, including studies that evaluated women with a history of failed fertility treatments with conventional methods, additionally gestational losses and decreased endometrial thickness. In the literature search we used databases such as Medline, Scopus and Google Scholar that helped to extract the necessary papers for the exploration. Additionally, we used Boolean terms to search for information that was later used in the PRISMA statement to synthesize the results obtained. In the reviewed studies we found that hormonal treatment combined with autologous platelet-rich plasma application could increase endometrial thickness and positively impact fertility of treated woman. Finally, it is concluded that more quality studies and larger number of patients in which PRP is included as an additional treatment to hormonal therapy are needed to determine its impact on endometrial thickness.

Resumen Se realiza una revisión exploratoria que muestra estudios donde se evalúa el uso del plasma rico en plaquetas (PRP) autólogo como tratamiento adicional al tratamiento hormonal para aumentar el grosor del endometrio en pacientes con infertilidad relacionada con el grosor endometrial. Realizamos una búsqueda de la literatura desde el año 2015 hasta el 2024 incluyendo estudios que evaluaron mujeres con antecedentes de tratamientos de fertilidad fallidos con métodos convencionales, adicionalmente pérdidas gestacionales y disminución del grosor del endometrio. En la búsqueda de la literatura utilizamos bases de datos como Medline, Scopus y Google Scholar que ayudaron a extraer los trabajos necesarios para la exploración. Adicionalmente usamos términos booleanos para la búsqueda de información que posteriormente se utilizaron en la declaración PRISMA para sintetizar los resultados obtenidos. En los estudios revisados encontramos que el tratamiento hormonal combinado con la aplicación de PRP autólogo podría aumentar el grosor endometrial e impactar positivamente en la fertilidad de las pacientes tratadas. Finalmente, se concluye que son necesarios más estudios de calidad y mayor número de pacientes en los que se incluya el PRP como tratamiento adicional a la terapia hormonal para determinar su repercusión sobre el grosor endometrial.

RESUMEN Introducción: El precursor del factor de crecimiento epidérmico está inmerso en el contexto de la membrana plaquetaria. El procedimiento actual para obtener suero implica un reto para una interpretación suficiente del significado biológico de sus valores. Objetivo: Describir el efecto de la modificación del procedimiento de obtención de muestra para la determinación del factor de crecimiento epidérmico, y las posibles implicaciones biológicas de sus resultados. Método: Se llevó a cabo un estudio experimental in vitro, con muestras de sangre de sujetos con antecedentes de salud aparente, en edad adulta. Se determinaron los valores de factor de crecimiento epidérmico sérico con el kit de UMELISA-EGF según recomendaciones del fabricante; la variación experimental en la obtención de la muestra se realizó según recomendaciones de SAMERSAC para plasma rico en plaquetas, con ajustes. Resultados: En la casuística, la edad promedio fue de 45.89± 3.35 años, con rango de 27-62 años. Predominó el sexo femenino en una razón 1.8:1. La aplicación del procedimiento experimental evidenció diferencias significativas y notorias entre los valores de EGF (g=17.7; p=0.0001-23); no se evidenció correlación lineal entre los valores (r= 0.1305; p= 0.6843). El valor de “reserva”, fue en promedio de 1498.61±160.68 pg*mL-1, observándose diferencias de efecto grande, así como un comportamiento similar a un valor de transición. Los datos observados sugieren, a posteriori, un efecto de consumo en el curso de la lisis plaquetaria en condiciones estándar. Conclusiones: Los valores del factor de crecimiento epidérmico son superiores luego del aislamiento de las plaquetas; la existencia de una reserva intraplaquetaria de factor de crecimiento epidérmico es plausible.

ABSTRACT Introduction: Epidermal growth factor precursor is immersed in the context to Platelets membrane. The regular procedure to obtain serum implies a barrier to biological understanding of the theoretical meaning of its values. Objective: To describe the effect to modified procedure to measure sample epidermal growth factors and the possible biological implications for this. Method: An in vitro experimental study was carried put, with blood samples from individuals with apparent health history, in adulthood. Serum epidermal growth factor precursor values were determined with the UMELISA-EGF kit recommended by the manufacturer; the experimental variation in sample collection was carried out according to SAMERSAC recommendations for platelet-rich plasma, with adjustments. Results: The average age was 45.89± 3.35 years, in a range of 27 to 62 years. The female sex predominates in a 1.8:1 ratio. Application of the experimental procedure showed significant and notable differences between the epidermal growth factor values (g=17.7; p=0.0001-23); no linear correlation was evident between the values (r= 0.1305; p= 0.6843). The "reserve" value was on average 1498.61 ±160.68 pg*mL-1, observing large effect differences, as well as a similar behavior to a transition value. The observed data suggest, a posteriori, consumption effect on the course of platelet lysis under standard conditions. Conclusions: Epidermal growth factor values are higher after platelet isolation; the existence of an intraplatelet reserve of epidermal growth factor is plausible.

ABSTRACT Introduction: Obesity and overweight constitute global public health challenges, negatively impacting more than one billion people. They are accompanied by significant psychological and social consequences, including the stigma associated with weight, with severe repercussions on mental and physical well-being. Health professionals are directly involved with this problem; however, few studies address weight stigma in Chile. Objective: To examine the association between nutritional status (BMI) and the level of weight stigma in university students of health careers in the region of Ñuble, Chile. Methodology: Cross-sectional descriptive observational design. The GAMS-27 scale was applied to assess weight stigma and BMI to evaluate the nutritional status of 156 students of health careers. Chi2 tests of independence and binary logistic regression were applied to evaluate the association between the variables, with the R statistical package version 4.3.0. Results: A statistically significant association was found between weight stigma and nutritional status (p=0.0145). Presenting normal BMI (p=0.0163) and being female (p=0.0156) were associated with a higher probability of presenting weight bias. Conclusions: The present study identified a statistically significant association between weight stigma and nutritional status in college students. It is vital to promote education regarding weight stigma in college students to avoid bias and improve respect for future users.

RESUMEN Introducción: El uso de sangre de cordón umbilical (CU) tiene muchas ventajas en la medicina regenerativa. El plasma rico en plaquetas (PRP) es una fuente de factores de crecimiento en donde su dinámica en el tiempo ha sido evaluada en sangre periférica, sin embargo, su evaluación en sangre de CU no ha sido estudiada. Objetivo: Evaluar la cinética de la liberación del PDGF-BB del PRP obtenido de sangre de CU. Materiales y métodos: Se realizó un estudio experimental in vitro. Se recolectaron muestras de sangre de CU de 6 partos a término de gestantes sanas que tenían entre 18 y 36 años y que acudieron al Hospital Cayetano Heredia en Lima-Perú. Las muestras obtenidas se centrifugaron a 900 g durante 10 minutos para preparar el PRP. La activación se realizó con gluconato de calcio (GLu.Ca) al 10% y se dividió en 4 alícuotas: (i) sin activador; (ii) 1 hora; (iii) 24 horas; (iv) 48 horas. La cuantificación del PDGF-BB fue evaluada mediante el método de ELISA. Resultados: La media (desviación estándar) de la concentración de PDGF-BB medida a la hora, 24 horas y 48 horas fue 6127.9 ± 101.6 pg/mL, 6197.5 ± 34 pg/mL y 6176.8 ± 63.3 pg/mL, respectivamente. La cinética del PDGF-BB liberado por el PRP mostró valores constantes durante las 48 horas. Conclusiones: La liberación de PDGF-BB del PRP obtenido de sangre de CU se indujo rápidamente y se mantuvo constante y de forma mantenida durante las primeras 48 horas luego de su activación.

ABSTRACT Introduction: Platelet -rich fibrin (PRF) is a biomaterial derived from blood plasma that has gained considerable attention in the field of regenerative medicine and dentistry in particular. Objective: To evaluate the use of platelet -rich fibrin in regenerative endodontics. Methods: A descriptive, observational and transverse study was carried out in which the 2020 prism methodology was used. The search focused on the bases of Scielo, Pubmed, Scopus, as well as documents and websites that contributed to the theoretical construct of the investigation from the year 2013 until 2023. Results: Regenerative endodontics, such as the new field within dentistry, aims to restore the tissues of damaged or infected tooth pulp and promote the regeneration of dentin, root structures and surrounding tissues. In addition, it seeks to provide an alternative treatment for immature teeth with open apexes or teeth with pulp necrosis and periapical lesions. Platelet rich fibrin is a concentrate of platelets and growth factors obtained from the patient's blood and is a relevant technique in regenerative medicine and dentistry due to its potential to accelerate the healing and regeneration of tissues. Conclusions: Regenerative endodontics with the use of platelet -rich fibrin creates a matrix that supports migration and differentiation of stem cells, which are essential for tissue regeneration

RESUMEN Introducción: La fibrina rica en plaquetas (PRF) es un biomaterial derivado del plasma sanguíneo que ha ganado considerable atención en el ámbito de la medicina regenerativa y la odontología en particular. Objetivo: Evaluar el empleo de la fibrina rica en plaquetas en la endodoncia regenerativa. Métodos: Se realizó un estudio descriptivo, observacional y transversal en el que se empleó la metodología PRISMA 2020. La búsqueda se centró en las bases de Scielo, Pubmed, Scopus, así como documentos y sitios web que aportaron al constructo teórico de la investigación desde el año 2013 hasta el 2023. Resultados: La endodoncia regenerativa, como nuevo campo dentro de la odontología, tiene como objetivo restaurar los tejidos de la pulpa dental dañados o infectados y promover la regeneración de la dentina, las estructuras de la raíz y los tejidos circundantes. Además, busca proporcionar un tratamiento alternativo para los dientes inmaduros con ápices abiertos o dientes con necrosis pulpar y lesiones periapicales. La fibrina rica en plaquetas es un concentrado de plaquetas y factores de crecimiento obtenidos a partir de la sangre del paciente y resulta una técnica relevante en la medicina regenerativa y la odontología debido a su potencial para acelerar la curación y regeneración de tejidos. Conclusiones: La endodoncia regenerativa con el uso de la fibrina rica en plaquetas crea una matriz que respalda la migración y diferenciación de células madre, que son esenciales para la regeneración del tejido.

ABSTRACT Introduction: Due to its properties, platelet preparations are useful in the control of bacterial growth in the burned skin. Objective: To analyze physiological mechanisms that platelets may have to control the infection, when applied in the local burning cure. Methods: A review of the publications of the years 2008-2023, in English and Spanish, on the regenerative treatment with platelet-rich plasm and the platelet lysate of open and closed injuries with the presence of infectious agents, were carried out. Search was carried out in Medline database, through the Pubmed, and Scielo, through its search engine; Academic Google was also used. The most used keywords were infection and platelets. 63 national and international publications were reviewed, 32 of which were relevant for this work. Conclusions: Platelet rich formulations have microbicidal action against an important number of bacteria that colonize burns, also stimulate the epithelization of damaged skin.

RESUMEN Introducción: Por sus propiedades, las preparaciones plaquetarias son útiles en el control del crecimiento bacteriano en la piel del quemado. Objetivo: Analizar los mecanismos fisiológicos que pueden tener las plaquetas para controlar la infección cuando son aplicadas en la cura local de quemaduras. Métodos: Se realizó revisión de las publicaciones de los años 2008-2023, en inglés y español, sobre el tratamiento regenerador con plasma rico en plaquetas y el lisado de plaquetas de lesiones abiertas y cerradas con presencia de agentes infecciosos. La búsqueda se realizó en las bases de datos Medline, a través del buscador PubMed y SciELO, a través de su buscador. Se utilizó también Google Académico. Las palabras clave más empleadas fueron infección y plaquetas. Se revisaron 63 publicaciones nacionales e internacionales de las cuales 32 fueron pertinentes para este trabajo. Conclusiones: Las formulaciones ricas en plaquetas tienen acción microbicida contra un importante número de bacterias que colonizan las quemaduras y estimulan, además, la epitelización de la piel dañada.

Abstract One of the crucial aspects to be considered for successful in vitro production (IVP) of embryos is the composition of the various media used throughout the stages of this reproductive biotechnology. The cell culture media employed should fulfill the metabolic requirements of both gametes during oocyte maturation and sperm development, as well as the embryo during its initial cell divisions. Most IVP protocols incorporate blood serum into the media composition as a source of hormones, proteins, growth factors, and nutrients. Numerous studies have suggested Platelet-Rich Plasma (PRP) as a substitute for fetal sera in cell culture, particularly for stem cells. Therefore, the objective of this study is to assess the potential use of PRP as a replacement for fetal bovine serum (FBS) during oocyte maturation for in vitro production of bovine embryos. During in vitro maturation (IVM), cumulus-oocyte complexes (COCs) were allocated into the following experimental groups: Group G1 (IVM medium with 5% PRP); Group G2 (MIV medium with 5% PRP and 5% SFB); Group G3 (MIV medium with 5% SFB); and Group G4 (MIV medium without either PRP or SFB). Subsequently, the cumulus-oocyte complexes were fertilized with semen from a single bull, and the resulting zygotes were cultured for seven days. Cleavage and blastocyst formation rates were assessed on days 2 and 7 of embryonic development, respectively. The quality of matured COCs was also evaluated by analyzing the gene expression of HSP70, an important protein associated with cellular stress. The results demonstrated that there were no significant differences among the experimental groups in terms of embryo production rates, both in the initial cleavage stages and blastocyst formation (except for the G4 group, which exhibited a lower blastocyst formation rate on D7, as expected). This indicates that PRP could be a cost-effective alternative to SFB in the IVP of embryos. (IVP biotechnology development divisions hormones proteins factors nutrients PlateletRich Platelet Rich (PRP cells Therefore FBS (FBS IVM, IVM , (IVM) cumulusoocyte cumulus (COCs G 5 PRP) MIV SFB) SFB. . Subsequently bull respectively HSP70 HSP stress except group D7 D expected. expected expected) costeffective cost effective HSP7

Abstract The use of platelet-rich plasma (PRP) and adipose-derived stromal cells (ADSC) have been investigated as a form of wound healing enhancement. The objective of this work was to evaluate the association of red propolis (RP) and PRP as inducers of ADSC for application in tissue regeneration. Adipose tissue post-collection and post-cryopreservation was isolated with type II collagenase, characterized by flow cytometry, and differentiated into osteogenic, chondrogenic and adipose cell. The viability of ADSC was evaluated when exposed to different concentrations of RP using the MTT and trypan blue assay. Acridine orange and ethidium bromide (AO/EB) was performed to evaluate cell death events. Horizontal migration methods were investigated in ADSC using autologous and homologous PRP associated with RP (PRP/RP). All assays were processed in triplicate. Flow cytometry and cellular differentiation showed that type II collagenase was effective for isolating ADSC post-collection and post-cryopreservation. RP extracts at concentrations of up to 50 μg.mL-1 presented no cytotoxic effects. Association of PRP and RP at 25 and 50 μg.ml-1 influenced ADSC migration, with total closure on the seventh day after exposition. The results here presented could stimulate proliferation of ADSC cells that may contribute directly or indirectly to the reconstructive process of tissue regeneration. plateletrich platelet rich (PRP adiposederived derived (ADSC enhancement (RP regeneration postcollection post collection postcryopreservation cryopreservation osteogenic assay AO/EB AOEB AO EB (AO/EB events PRP/RP. PRPRP PRP/RP . (PRP/RP) triplicate postcryopreservation. cryopreservation. 5 μg.mL1 μgmL1 μgmL μg.mL 1 μg mL μg.mL- effects 2 μg.ml1 μgml1 μgml μg.ml ml μg.ml- exposition (PRP/RP mL1 ml1

Aim Venous blood derivatives (VBDs) have been suggested as substitutes for Fetal Bovine Serum (FBS) to improve the clinical transition of cell-based therapies. The literature is not clear about which is the best VBDs substitute. The present study aimed to evaluate the influence of VBDs on cell viability and describe a new method to seed these cells in a 3D Platelet-Rich Fibrin (PRF). Methods Blood was processed to obtain Platelet-Poor Plasma from PRF (P-PRF), Human Serum (HS), Platelet-Poor Plasma from PRP (P-PRP), activated-PRP (a-PRP), and Platelet lysate (PL). Cells were supplemented with each VBD at 10% and FBS at 10% was the control. Cell viability (fibroblast 3T3/NIH) test was evaluated with MTT assay in two ways: i) cell-seeded and expanded with VBD; ii) cell-seed with FBS and expanded with VBD. To seed the Fibrin construct, cells were suspended in PBS and dropped into the blood sample before performing Choukroun’s protocol for PRF. Constructs were cultured for 7 days in VBD supplements and FBS. Histological and Immunohistochemical analysis with vimentin was performed. Cell viability was analyzed by one-way ANOVA. Results VBD’s production time was very heterogeneous. Cells expanded in HS and a-PRP has grown faster. VBD-supplemented culture media provided cell culture highly sensible to trypsin/EDTA 0.25%. Cells seeded and expanded with VBD presented viability comparable to FBS in HS, a-PRP, and P-PRP (p>0.05) and lower in P-PRF and PL groups (p<0.05). The viability of cell seed with FBS and expanded with VBD was similar between P-PRF, a-PRP, PL, and FBS (p>0.05) and lower in HS and P-PRP (p<0.005). PRF-seeded cells showed a positive expression of vimentin and were able to maintain all cells supplemented with VBD. Conclusion VBD supplements were able to maintain fibroblast cells in 2D and 3D cultures. The new method of the fibrin-cell construct was efficient to insert the cells into the fibrin network. (VBDs (FBS cellbased based therapies substitute D PlateletRich Rich . (PRF) PlateletPoor Poor PPRF, PPRF P , (P-PRF) (HS) PPRP, PPRP (P-PRP) activatedPRP activated aPRP, aPRP (a-PRP) PL. (PL) 10 control 3T3/NIH 3T3NIH TNIH 3T3 NIH T ways i cellseeded ii cellseed Choukrouns Choukroun s performed oneway one way ANOVA heterogeneous faster VBDsupplemented trypsinEDTA trypsin EDTA 025 0 25 0.25% PRP, p>0.05 p005 p 05 (p>0.05 p<0.05. p<0.05 (p<0.05) PRF, p<0.005. p0005 p<0.005 005 (p<0.005) PRFseeded cultures fibrincell network (PRF (P-PRF (HS (P-PRP (a-PRP (PL 1 3T 02 2 0.25 p>0.0 p00 (p>0.0 p<0.0 (p<0.05 p000 p<0.00 00 (p<0.005 0.2 p>0. p0 (p>0. p<0. (p<0.0 (p<0.00 0. p>0 (p>0 p<0 (p<0. p> (p> p< (p<0 (p (p<

Abstract Platelet rich plasma and ozone therapy have been suggested in the clinical setting as promising and adjuvant therapies for managing the symptoms related to the facial aging. However, there is a lack of evidence about the effects of combining both therapies on rejuvenation. Thus, the aim of the present study was to evaluate and to compare the safety and efficacy of both therapies in facial aging. A single-center prospective pilot study was conducted. Female participants, aged 40 to 60 years, were randomized into 2 groups: participants received the platelet rich plasma treatment (PRP); participants received the therapy with platelet rich plasma associated with ozone therapy (PRPOT). Interventions were performed using the facial intradermal technique in 42 pre-established points, for 4 sessions with an interval of 15 days between them. After analyses performed using photographic records, assessment of frontal, periorbital, nasolabial and marionette wrinkles (Wrinkle Assessment Scale), self-esteem assessment (Brazilian Version of Rosenberg’s Self-Esteem Scale) and assessment of patient satisfaction (FACE-Q) can be suggested that the platelet rich plasma protocol performed in the present study, as well as its association with volume-controlled ozone therapy, proved to be safe and potentially promising modalities in the treatment of facial aging. aging However rejuvenation Thus singlecenter single center conducted 6 years groups PRP (PRP) PRPOT. PRPOT . (PRPOT) preestablished pre established points 1 them records frontal periorbital Wrinkle Scale, Scale , selfesteem self esteem Brazilian Rosenbergs Rosenberg s SelfEsteem Self Esteem FACEQ FACE Q (FACE-Q volumecontrolled volume controlled (PRP (PRPOT

RESUMEN Introducción: El plasma rico en plaquetas es un producto derivado de la sangre, rico en péptidos y proteínas de señalización intercelular, así como citoquinas capaces de intervenir en cada una de las etapas de la regeneración de varios tejidos. Objetivo: Evaluar la efectividad del tratamiento con plasma rico en plaquetas y leucocitos en pacientes con fisura anal secundaria que asistieron a la consulta de Coloproctología del Hospital Docente Provincial Oncológico María Curie de Camagüey en el período de enero de 2020 a enero de 2023. Métodos: Se realizó un estudio cuasiexperimental. El universo lo conformaron todos los pacientes que acudieron a consulta en ese período. La muestra no probabilística intencionada la conformaron 130 pacientes adultos con diagnóstico de fisura anal secundaria que recibieron tratamiento con plasma rico en plaquetas y leucocitos. Resultados: El tiempo de cicatrización de la fisura anal permitió corroborar la efectividad del tratamiento; además, la mayoría de los pacientes evolucionaron de forma favorable. El dolor fue la complicación más visible pues presentó significación estadística al establecer la relación entre las variables. Se demostró que el tratamiento con plasma rico en plaqueta y leucocitos en pacientes con fisura anal secundaria es efectivo y seguro en la evaluación final del tratamiento. Conclusiones: Lo expuesto permite considerar que los pacientes tratados obtuvieron buenos resultados al hacer uso del plasma rico en plaquetas y leucocitos. Por consiguiente, tiene un resultado positivo en no mostrar complicaciones y una alta posibilidad de que el paciente tratado evolucione en mejor condición.

ABSTRACT Introduction: Platelet-rich plasma is a blood-derived product, rich in peptides and intercellular signaling proteins, as well as cytokines capable of intervening in each of the stages of regeneration of various tissues. Objective: To assess the effectiveness of treatment with platelet- and leucocyte-rich plasma in patients with secondary anal fissure who attended the coloproctology office at Hospital Docente Provincial Oncológico María Curie of Camagüey from January 2020 to January 2023. Methods: A quasiexperimental study was carried out, whose universe consisted of all the patients who came for consultation during that period. The nonprobabilistic purposive sample consisted of 130 adult patients with a diagnosis of secondary anal fissure who received treatment with platelet- and leucocyte-rich plasma. Results: The healing time of the anal fissure allowed corroborating the effectiveness of the treatment; in addition, most of the patients evolved favorably. Pain was the most visible complication since it presented statistical significance when the relationship between the variables were established. Treatment with platelet- and leucocyte-rich plasma in patients with secondary anal fissure proved effective and safe in the final assessment of the treatment. Conclusions: The above allows considering that the treated patients obtained good outcomes when making use of platelet- and leucocyte-rich plasma. Therefore, it has a positive outcome, not showing complications and a high possibility for the treated patient to evolve in a better condition.

Abstract Objective: This study was carried out to investigate the effect of autologous platelet-rich plasma (PRP) on intra-abdominal adhesion at the cesarean section incision line in the uterus. Material and methods: As experimental animals 16 white New Zealand rabbits, 5-months-old, unmated, were used. Animals were divided into two groups the control group and PRP application group. In each group, a transverse incision was made to the uterus to mimic the cesarean section and sutured. Relaparotomy was performed 21 days after the first operation. Results: When the groups were evaluated in terms of inflammation, there was a significant difference between the two groups. When the groups were evaluated in terms of Mason’s Trichrome staining and fibrosis, There was a significant difference between groups. When the groups were evaluated in terms of vascular endothelial growth factor-1, there was also a significant difference between the groups. In an experimental rabbit uterine horn adhesion model, PRP is effective in preventing post-operative adhesion formation. Conclusions: This result may guide clinical studies using autologous PRP to prevent post-operative adhesion formation after gynecological operations.

Resumen Objetivo: Este estudio se llevó a cabo para investigar el efecto del plasma rico en plaquetas (PRP) autólogo sobre la adhesión intraabdominal en la línea de incisión de la cesárea en el útero. Material y métodos: Como animales de experimentación se utilizaron 16 conejos blancos de Nueva Zelanda, de 5 meses de edad, sin aparear. Los animales se dividieron en dos grupos como grupo de control y grupo de aplicación de PRP. En cada grupo, se hizo una incisión transversal al útero para imitar la cesárea y se suturó. La relaparotomía se realizó 21 días después de la primera operación. Resultados: Cuando los grupos se evaluaron en términos de inflamación, hubo una diferencia significativa entre los dos grupos. Cuando los grupos se evaluaron en términos de tinción MT y fibrosis, hubo una diferencia significativa entre los grupos. Cuando los grupos se evaluaron en términos de VEGF-1, también hubo una diferencia significativa entre los grupos. En un modelo experimental de adherencia al cuerno uterino de conejo, el PRP es eficaz para prevenir la formación de adherencias posoperatorias. Conclusiones: Este resultado puede guiar los estudios clínicos que utilizan PRP autólogo para prevenir la formación de adherencias postoperatorias después de operaciones ginecológicas.

Resumen Introducción: El plasma rico en plaquetas (PRP) se encuentra en desarrollo desde los años 80, asociado con aplicaciones en medicina cardíaca, traumatológica y dermatológica. El PRP es una preparación autóloga, con una concentración de plaquetas por sobre el valor normal en sangre periférica, que se obtiene a partir de su centrifugación siguiendo diferentes protocolos que fueron valorados en distintas revisiones sistemáticas. El plasma luego se activa con trombina o cloruro cálcico, o por lisis física (ultrasonido o frizado). El PRP contiene factores de crecimiento, citoquinas y proteínas de adhesión que, al aplicarse en la lesión, favorecen la hemostasia, la síntesis de tejido conectivo y la revascularización. El producto se aplica en solución o gel. Se intentaron diferentes clasificaciones del PRP para estandarizarlo, sin éxito. Se conceptualizó la receta del PRP para aplicación clínica, que presentó las siguientes características: valores altos de plaquetas, disminución de la contaminación de glóbulos rojos, presencia de neutrófilos y leucocitos para el éxito terapéutico. Protocolo y casos: Se presenta el protocolo de preparación de PRP de centrifugación única de 7 minutos a 1400 rpm, con activación por medio de gluconato de calcio según la siguiente proporción: 3 ml plasma/0.4 ml gluconato cálcico. Además, se informan tres casos de úlceras de pie diabético de nuestra institución. Conclusión: El PRP no presenta complejidad en su preparación y aplicación, por lo que es factible de realizar en el primer nivel de atención que cuente con los recursos materiales y profesionales con conocimiento en el abordaje de heridas crónicas.

Abstract Introduction: Platelet-rich plasma (PRP) has been under development since the 1980s, associated with applications in cardiac, traumatological and dermatological medicine. PRP is an autologous preparation with a platelet concentration above the normal value in peripheral blood that is obtained from its centrifugation following different protocols that were evaluated in different systematic reviews. The Plasma is then activated with thrombin or calcium chloride, or by physical lysis (ultrasound or friz). PRP contains growth factors, cytokines, and adhesion proteins that, when applied to the lesion, favor hemostasis, connective tissue synthesis, and revascularization. The product is applied in solution or gel. Different classifications of the PRP were tried to standardize it without success. The PRP recipe for clinical application was conceptualized, which presented the following characteristics: high platelet values, decreased red blood cell contamination, presence of neutrophils and leukocytes for therapeutic success. Protocol and cases: The PRP preparation protocol for single centrifugation for 7 minutes at 1400 rpm with activation through calcium gluconate are presented according to the following ratio: 3 ml plasma/0.4 ml of calcium gluconate. Three cases of diabetic foot ulcers from our institution are reported. Conclusion: The PRP does not present complexity in its preparation and application, so it is feasible to perform it in the first level of care that has the material and professional resources with knowledge in the approach to chronic wounds.

RESUMEN Introducción: Las enfermedades benignas de recto son muy frecuentes en la práctica médica habitual en todos los niveles de atención de salud. Dentro de estos procesos se reconoce que la fisura anal es uno de los más comunes. Objetivo: Caracterizar a los pacientes tratados con plasma rico en plaquetas y leucocitos por fisura anal secundaria en la provincia Camagüey en el período comprendido entre septiembre del 2020 y septiembre del 2022. Métodos: Se realizó un estudio cuasiexperimental, cuyo universo lo conformaron los pacientes con el diagnóstico de fisura anal secundaria que asistieron a la consulta de Coloproctología del Hospital Provincial Docente Oncológico María Curie. Se estableció una muestra no probabilística a criterio de los autores conformada por 132 pacientes adultos, quienes recibieron tratamiento con plasma rico en plaquetas y leucocitos por fisura anal secundaria en la provincia Camagüey. Resultados: Con respecto al sexo y a la edad, se observó una mayor frecuencia del grupo de 40 a 49 años y predominó el sexo femenino. El síntoma de mayor frecuencia fue el dolor, mientras que en la etiología prevaleció la proctitis facticia. En la mayoría de los casos estudiados la fisura anal cicatrizó en menos de 15 días y en cuanto a la evaluación final del tratamiento solo la minoría empeoró. Conclusiones: La caracterización de la muestra en estudio permite afirmar que el tratamiento con plasma rico en plaquetas y leucocitos tiene beneficios para los pacientes con fistulas perianales y su calidad de vida.

ABSTRACT Introduction: Benign rectal diseases are very frequent in routine medical practice at all levels of health care. Among these processes, anal fissure is recognized as one of the most common. Objective: To characterize patients treated with platelet-rich plasma and leukocytes for secondary anal fissure in Camagüey Province between September 2020 and September 2022. Methods: A quasiexperimental study was carried out, whose universe was made up of patients with the diagnosis of secondary anal fissure who attended the coloproctology office at Hospital Provincial Docente Oncológico María Curie. A nonprobabilistic sample was established according to the authors’ criterions, made up of 132 adult patients who received treatment with platelet-rich plasma and leukocytes for secondary anal fissure in Camagüey Province. Results: Regarding sex and age, a higher frequency was observed in the group 40 to 49 years old and the female sex was predominant. The most frequent symptom was pain, while the prevailing etiology was factitious proctitis. In most of the cases under study, the anal fissure healed in less than 15 days and, regarding the final assessment of the treatment, only the minority worsened. Conclusions: The characterization of the sample under study allows to affirm that treatment with platelet-rich plasma and leukocytes has benefits for patients with perianal fistulas and their quality of life.

Abstract Background and objective. The combination of minimally invasive techniques is very common to treat aging skin. The objective of this trial is to evaluate an intensive treatment protocol for facial rejuvenation, in a single, combined and sequential session, using high intensity focused ultrasound (HIFU), fractional CO2 laser and autologous platelet rich plasma (PRP). Methods. Pilot trial to evaluate the efficacy and safety of the combined treatment in a selected sample of 15 patients (phototypes III-V) with evident signs of facial aging. Efficacy was assessed blinded by 2 independent experts by comparing photographs before treatment and 3 months later. The variables analyzed (degree of pain, laxity, global rejuvenation and satisfaction) were quantified using ordinal quantitative or qualitative scales. Safety is assessed by follow-up notes in medical records. Results. Significant improvements in laxity reduction were achieved in the upper, middle, and lower thirds of the face (p=0.026, p=0.011, p=0.003). On the scale of global aesthetic improvement, according to the expert evaluators, 14 participants (93%) consider themselves "improved" or "very improved". All patients recognized some kind of improvement, 9 indicated being "very satisfied", 3 "satisfied" and 3 "moderately satisfied". The treatment was painful, but sufficiently tolerated by the majority of patients. There were no unexpected side effects or major complications, with good postoperative recovery. In general, the patients returned to their work activities 10 days after treatment. Conclusions. The efficacy/safety profile of the tested method has been very favorable in the series investigated.

Resumen Introducción y objetivo. La combinación de técnicas mínimamente invasivas es muy frecuente para tratar el envejecimiento de la piel. El objetivo de este ensayo es evaluar un protocolo de tratamiento intensivo para rejuvenecimiento facial en una sesión única, combinada y secuencial, empleando ultrasonidos focalizados de alta intensidad (HIFU), láser de CO2 fraccionado y plasma rico en plaquetas (PRP) autólogo. Material y método. Ensayo piloto para evaluar la eficacia y seguridad del tratamiento combinado en una muestra seleccionada de 15 pacientes (fototipos III-V) con signos evidentes de envejecimiento facial. La eficacia fue evaluada de forma enmascarada por 2 expertos independientes mediante comparación de fotografías antes del tratamiento y 3 meses después. Las variables analizadas (grado de dolor, laxitud, rejuvenecimiento global y satisfacción) se cuantificaron mediante escalas ordinales cuantitativas o cualitativas. La seguridad se evaluó mediante anotaciones de seguimiento en las historias clínicas. Resultados. Obtuvimos mejorías significativas en la reducción de la laxitud en los tercios superior, medio e inferior de la cara (p=0.026, p=0.011, p=0.003). En la escala de mejoría estética global, según los evaluadores expertos, 14 participantes (93%) se consideraron “mejorados” o “muy mejorados”. Todos los pacientes reconocieron algún tipo de mejoría, 9 indicaron estar “muy satisfechos”, 3 “satisfechos” y 3 “moderadamente satisfechos”. El tratamiento fue doloroso, pero suficientemente tolerado por la mayoría de pacientes. No aparecieron efectos adversos inesperados ni complicaciones importantes, con buena recuperación postoperatoria. En general los pacientes se reincorporaron a sus actividades laborales 10 días después del tratamiento. Conclusiones. El perfil de eficacia/seguridad del método ensayado ha sido muy favorable en la serie investigada.