RESUMO O aumento da fertilidade do solo no cultivo de hortaliças apresenta desafios, notadamente usando adubo verde de espécies espontâneas do bioma Caatinga, como a flor-de-seda (Calotropis procera [Ait.] R. Br.). Este estudo teve como objetivo avaliar e otimizar as eficiências física e econômica da rúcula em monocultivo, influenciadas por quantidades variáveis de biomassa de flor-de-seda. Um delineamento em blocos casualizados com sete tratamentos e cinco repetições foi utilizado. Os tratamentos consistiram de diferentes quantidades de biomassa de C. procera (20, 40, 60, 80 e 100 t ha-1 em base seca), juntamente com dois tratamentos adicionais em cada bloco: um controle (sem fertilização) e um com fertilização mineral. A cultivar de rúcula "Cultivada" foi adubada para máxima eficiência produtiva usando 63,31 t ha-1 de biomassa seca de C. procera, rendendo 8,45 t ha-1. A maior eficiência agroeconômica otimizada, refletindo uma renda líquida de R$ 111.007,64 por hectare, foi alcançada com 59,26 t ha-1 de biomassa seca de C. procera. A taxa de retorno foi de R$ 4,65 para cada real investido, com um índice de rentabilidade de 77,38%. Assim, utilizar biomassa de C. procera como adubo verde é uma tecnologia viável para produtores de rúcula em regiões semiáridas. desafios Caatinga flordeseda flor seda Calotropis Ait. Ait [Ait. R Br.. Br Br. . Br.) monocultivo flordeseda. seda. utilizado C 20, 20 (20 40 60 8 10 ha1 ha 1 ha- seca, , seca) bloco sem mineral Cultivada "Cultivada 6331 63 31 63,3 845 45 8,4 ha1. 1. otimizada 11100764 111 007 64 111.007,6 hectare 5926 59 26 59,2 465 4 65 4,6 investido 7738 77 38 77,38% Assim semiáridas [Ait 2 (2 6 633 3 63, 84 8, 1110076 11 00 111.007, 592 5 59, 46 4, 773 7 77,38 ( 111007 0 111.007 77,3 11100 111.00 77, 1110 111.0 111.

ABSTRACT Enhancing soil fertility in vegetable cultivation presents challenges, notably using green manure from spontaneous species in the Caatinga biome, such as roostertree (Calotropis procera [Ait.] R. Br.). This study aimed to evaluate and optimize the physical and economic efficiencies of monocropped arugula, as influenced by varying amounts of roostertree biomass. We employed a randomized block design with seven treatments and five replications. Treatments involved different quantities of C. procera biomass (20, 40, 60, 80, and 100 t ha-1 on a dry basis), along with two additional treatments in each block: a control (no fertilization) and one with mineral fertilization. The "Cultivada" arugula cultivar was fertilized for maximum productive efficiency using 63.31 t ha-1 of C. procera dry biomass, yielding 8.45 t ha-1. The highest optimized agroeconomic efficiency, reflecting a net income of BRL 111,007.64 per hectare, was achieved with 59.26 t ha-1 of C. procera dry biomass. The return rate was BRL 4.65 for every real investment, with a profitability index of 77.38%. Thus, using C. procera biomass as green manure is a viable technology for arugula producers in semi -arid regions. challenges biome Calotropis Ait. Ait [Ait. R Br.. Br Br. . Br.) replications C 20, 20 (20 40 60 80 10 ha1 ha 1 ha- basis, basis , basis) no fertilization Cultivada "Cultivada 6331 63 31 63.3 845 8 45 8.4 ha1. 1. 11100764 111 007 64 111,007.6 hectare 5926 59 26 59.2 465 4 65 4.6 investment 7738 77 38 77.38% Thus arid regions [Ait 2 (2 6 633 3 63. 84 8. 1110076 11 00 111,007. 592 5 59. 46 4. 773 7 77.38 ( 111007 0 111,007 77.3 11100 111,00 77. 1110 111,0 111,

Summary: The state of Chiapas presents degradation processes of dif ferent kinds, as a consequence of the current established agricultural model. It is estimated that, in the near future, the surface of the state of Chiapas will present more than 55% degradation, generating changes in the physical, chemical and biological properties of the soil, af fecting the quality of life of human beings, mainly due to scarcity of food. The objective of this research was to evaluate the quality of the soils in three municipalities of the central depression of Chiapas, Mexico. The analysis were interpreted taking NOM-021-SEMARNAT-2000 as a reference and were classified according to the world reference base (WRB), version 2014. For the evaluation of soil quality, the sustainable use of land subindex (SUSS). 144 soil samples corresponding to 21 profiles were analyzed. Of the 21 profiles studied, 17 present poor soil qualities, two a sensitive quality, one acceptable quality and one with good quality. Only two soil groups presented good fertility and are classified as Vertisols and Phaeozems, which present good quality and capacity to maintain sustainable agricultural production in this region.

Resumen: El estado de Chiapas presenta procesos de degradación de diferente índole, como consecuencia del actual modelo agrícola establecido. Se estima que, en el futuro cercano, la superficie del estado de Chiapas presente más de 55% de degradación, generando modificaciones en las propiedades físicas, químicas y biológicas del suelo; afectando la calidad de vida de los seres humanos, principalmente por escasez de alimentos. El objetivo de esta investigación fue evaluar la calidad de los suelos en tres municipios de la depresión central de Chiapas, México. Los análisis se interpretaron tomando como referencia la NOM-021-SEMARNAT-2000 y se clasificaron de acuerdo con la world reference base (WRB), versión 2014, para la evaluación de calidad del suelo se utilizó el subíndice de uso sustentable del suelo (SUSS). Se analizaron 144 muestras de suelo correspondientes a 21 perfiles. De los 21 perfiles estudiados, 17 presentan calidad de suelo pobre, dos calidades sensibles, uno calidad aceptable y uno con calidad buena. Solo dos Grupos de suelo presentaron buena fertilidad y son clasificados como Vertisols y Phaeozems, los cuales presentan buena calidad y capacidad para mantener producción agrícola sostenible en esta región.

Resumen Introducción : La pandemia de COVID-19 ha ejercido impactos significativos en la sociedad, particularmente en niños, niñas y adolescentes (NNA), quienes han en frentado consecuencias sociales, educativas y de salud graves debido a la pérdida de sus cuidadores principales. Materiales y métodos : Se analizaron las tasas de fertilidad femenina y se realizaron estimaciones so bre la fertilidad masculina a fin de calcular los hijos menores de 18 años por grupo de edad. Se utilizó in formación demográfica y de mortalidad por infección por COVID-19 para calcular la orfandad resultante en Argentina durante el período 2020-2021, considerando la pérdida de uno o ambos progenitores o del cuidador principal abuelo/a. Resultados : Un total de 25 161 NNA argentinos perdieron a uno de sus cuidadores principales por el COVID-19 durante la pandemia. De ellos, 22 729 NNA quedaron huérfanos de madre o padre durante el pe riodo analizado. La mayoría de los casos (79.5%) estuvo relacionada con la muerte del padre. Se identificaron grupos de edad específicos con mayor incidencia de orfandad. La pérdida de abuelos cuidadores también fue significativa, afectando a 2432 NNA. Discusión : Los hallazgos subrayan la necesidad de políticas y programas específicos para abordar las nece sidades integrales de los NNA afectados por la orfandad durante la pandemia. Se destacan los posibles impactos a largo plazo en la salud física, mental, educativa y so cioeconómica de estos niños y adolescentes.

Abstract Introduction : The COVID-19 pandemic has had sig nificant impacts on society, particularly on children and adolescents, who have faced serious social, educational, and health consequences due to the loss of their pri mary caregivers. Materials and methods : Female fertility rates were analyzed, and estimates of male fertility were made to calculate the number of children under 18 years old by age group. Demographic and mortality information from COVID-19 infection was used to calculate the resulting orphanhood in Argentina during the period 2020-2021, considering the loss of one or both parents or the main caregiver grandparent. Results : A total of 25 161 Argentine children and adolescents lost one of their primary caregivers due to COVID-19 during the pandemic. Of these, 22 729 were orphaned by mother or father during the analyzed period. The majority of cases (79.5%) were related to the father’s death. Specific age groups with higher incidence of orphanhood were identified. The loss of caregiver grandparents was also significant, affecting 2432 chil dren and adolescents. Discussion : The findings underscore the need for specific policies and programs to address the compre hensive needs of children and adolescents affected by orphanhood during the pandemic. Potential long-term impacts on the physical, mental, educational, and so cioeconomic health of these children and adolescents are highlighted.

RESUMO O manejo inadequado de solos de pastagens na Amazônia resulta em predomínio de pastagens degradadas. Considerando as características naturais dessa região pode favorecer estratégias mais adequadas para sua sustentabilidade, o objetivo desse estudo foi avaliar as diferenças de atributos químicos e físicos de solos de pastagens e florestas no sul da Amazônia e identificar os atributos mais sensíveis de sua fertilidade. Além disso, sugerir práticas de manejo mais adequadas para essas pastagens em direção à sustentabilidade. Amostras de solo da camada de 0 a 0,20 m foram analisadas para determinação de pH, bases trocáveis (cálcio, magnésio e potássio), alumínio trocável, acidez potencial, fósforo, carbono orgânico, densidade aparente e textura. Os solos de pastagem tiveram maior pH, cálcio e densidade aparente do que os solos de floresta. No entanto, os solos de pastagem apresentaram menores teores de fósforo e matéria orgânica. Os estoques de carbono orgânico do solo também foram menores nas pastagens, com teores de 0,8 a 12 Mg ha-1 inferiores aos solos da floresta. Os atributos de fertilidade mais sensíveis ao manejo do solo nessas pastagens foram fósforo, pH, carbono orgânico e densidade aparente. A carência de nutrientes e de práticas de conservação do solo e da água têm contribuído para o estado de degradação dessas pastagens. Portanto, o manejo recomendado para essas pastagens deve visar a melhoria da matéria orgânica, redução da compactação, além da reposição e ciclagem de nutrientes. degradadas sustentabilidade disso 020 20 0,2 pH cálcio, (cálcio potássio, potássio , potássio) trocável potencial textura floresta entanto orgânica 08 8 0, 1 ha1 ha ha- Portanto compactação 02 2

ABSTRACT Inadequate management of pasture soils in the Amazon has resulted in a predominance of degraded pastures. Considering the natural characteristics of this region can favor more appropriate strategies for sustainability, the objective of this study was to evaluate the differences in the chemical and physical attributes of pasture and forest soils in the Southern Amazon and to identify the most sensitive attributes of their fertility. Additionally, this study suggests appropriate management practices for sustainable pastures. Soil samples from the 0 to 0.20 m layer were analyzed to determine pH, exchangeable bases (calcium, magnesium, and potassium), exchangeable aluminum, potential acidity, phosphorus, organic carbon, bulk density, and texture. Pasture soils had a higher pH, calcium content, and bulk density than forest soils. However, the pasture soils had lower phosphorus and organic matter content. The soil organic carbon stocks were also lower in pasture soils, with levels 0.8 to 12 Mg ha-1 lower than in forest soils. The fertility attributes most sensitive to soil management in these pastures were phosphorus, pH, organic carbon, and bulk density. The lack of nutrients and soil and water conservation practices have contributed to the degradation of these pastures. Therefore, the recommended management for these pastures should aim to improve the organic matter content, reduce compaction, and replenish and cycle nutrients. sustainability Additionally 020 20 0.2 pH calcium, (calcium magnesium potassium, potassium , potassium) aluminum acidity texture content However 08 8 0. 1 ha1 ha ha- Therefore compaction 02 2

BACKGROUND: Uterine fibroids (UFs) are benign uterine growths that significantly impact women's daily activities, quality of life, fertility and expenditure. AIM: This study aimed to provide in-depth insights into the lived experiences of women diagnosed with UFs. SETTING: The study was conducted in Eswatini health facilities across the four geographic regions. METHODS: An explorative qualitative design was used; participants with confirmed UFs diagnoses or surgery related to UFs were purposively recruited for focus group discussions. Data collection was guided by the revised Wilson and Cleary model of health-related quality of life (HRQOL), which was iteratively analysed using Braun and Clark's thematic analysis framework. RESULTS: Sixty participants with confirmed UFs diagnoses or surgery related to UFs were included in this study. Five themes emerged: awareness of the disease, symptoms experienced, treatment and care, perceptions and beliefs and health-seeking behaviour. Most participants experienced physical symptoms, abdominal pain and vaginal bleeding. Among the emotional and psychological consequences experienced by the majority of participants were fear, worry, tension and (perceived) disrupted body image. The majority of the participants lacked knowledge of UFs, and their perceptions and health-seeking behaviour differed. CONCLUSION: Findings showed that most participants experienced physical, psychological, emotional and social challenges. CONTRIBUTION: These experiences were influenced by lack of knowledge, symptoms experienced, poor treatment and care and perceptions and beliefs about UFs. Health education, client involvement, early diagnosis and effective treatment are recommended to improve the experiences of UFs.

ABSTRACT In ecotonal Cerrado areas, the addition of Amazonian and Atlantic Forest species mainly occurs through connections between forest areas and forest physiognomies. In this context, the biome is a unique region for research on the influence of geographical and historical factors on its biota. In tropical ecotonal regions, integrated communities can exist on soils that present variations in fertility, physical properties and depth, which results in heterogeneity of physiognomies. It was tested the hypothesis that edaphic conditions related to calcium availability are significantly related to species composition and structural parameters in two physiognomies. The study was conducted in the Cerrado of the Chapada das Mesas National Park (CMNP) in the city of Carolina, Northeast Brazil, and the sample universe consisted of 18 areas in two physiognomies. The initial hypothesis was confirmed. The results and inferences about the vegetation structure and physical-chemical parameters of the soil, suggest that the management for conservation of the CMNP must consider the particularities of the Cerrado physiognomies and the vegetation responses to environmental filters, such as edaphic conditions and associations with other organisms. context biota regions fertility depth (CMNP Carolina Brazil 1 confirmed physicalchemical chemical soil filters organisms

Resumo O plantio da cana-de-açúcar emprega métodos e processos produtivos de alto potencial de impacto no meio ambiente. Todavia, essas indústrias vêm investindo em tecnologias buscando soluções, que vão desde o descarte dos resíduos produzidos até a erradicação das queimadas. Objetivou-se nesse trabalho verificar a influência da vinhaça e colheita mecanizada nos aspectos físicos e químicos de um Argissolo Vermelho Amarelo distrófico de textura média, cultivados com cana-de-açúcar. Para as análises da densidade do solo, densidade de partícula e porosidade total, a coleta de dados foi realizada através de um experimento em Delineamento Inteiramente Casualizado, com esquema fatorial, em três repetições, a fim de se analisar a interação entre os fatores tratamentos x profundidades. Para tanto, foram utilizados quatro tratamentos (T1, T2, T3 e T4), em três profundidades (0 - 20 cm, 20 - 40 cm e 40 - 60 cm), e com três repetições. Os dados obtidos foram submetidos à ANOVA e comparados pelo teste de Tukey (p≤0,05) de probabilidade, utilizando-se o programa estatístico ASSISTAT 7.7 beta. Os resultados mostraram que a fertirrigação não influenciou na densidade do solo, densidade de partícula e porosidade total. Ainda em relação aos atributos físicos do solo, a colheita mecanizada influenciou no aumento da densidade do solo e porosidade total. A unidade experimental sem aplicação de vinhaça apresentou pH ácido e elevados teores de saturação por alumínio, ocasionando uma diminuição da disponibilidade de nutrientes para o solo. Contudo, pode-se concluir que as unidades experimentais tratadas com vinhaça tiveram um aumento na disponibilidade de nutrientes. Portanto, a adição de vinhaça pode ser uma estratégia importante na manutenção e aumento da fertilidade do solo a longo prazo em lavouras de cana-de-açúcar. canadeaçúcar cana açúcar ambiente Todavia soluções queimadas Objetivouse Objetivou média canadeaçúcar. açúcar. total Casualizado fatorial repetições tanto T1, T1 T (T1 T2 T4, T4 , T4) 0 ( 2 4 6 cm) p≤0,05 p005 p 05 (p≤0,05 probabilidade utilizandose utilizando 77 7 7. beta alumínio Contudo podese Portanto (T p≤0,0 p00 (p≤0,0 p≤0, p0 (p≤0, p≤0 (p≤0 p≤ (p≤ (p

Abstract Sugarcane cultivation methods and processes usually have negative impacts on the environment. However, this industry has been seeking more sustainable technologies, ranging from the adequate disposal of the organic residues produced to the elimination of pre-harvest burning and introduction of mechanized harvest. The objective of this work was to verify the influence of vinasse application and mechanized harvesting on the physical and chemical aspects of a dystrophic Red Yellow Argisol of medium texture, cultivated with sugarcane. For this, a field experiment in a Completely Randomized Design, with a factorial scheme was conducted. Four management treatments, with three replications, were evaluated: T1 - Without application of vinasse, burned cane followed by manual harvest, without irrigation; T2 - With application of vinasse, mechanized harvest, without irrigation; T3 - With application of vinasse, mechanized harvest, with irrigation; T4 - With application of vinasse, burned cane followed by manual harvest, without irrigation. Soil samples were collected in three depths (0 - 20 cm, 20 - 40 cm and 40 - 60 cm) and soil physical and chemical characteristics were determined. The data obtained were submitted to ANOVA and compared by the Tukey test (p≤0.05) of probability, using the statistical program ASSISTAT 7.7 beta. The results showed that fertigation with vinasse did not influence soil density, particle density, and total porosity. However, mechanized harvesting increased soil density and total porosity. The soil in the experimental unit without vinasse application presented a more acidic pH and high levels of aluminum saturation, causing a decrease in the availability of nutrients, and the soils treated with vinasse had an increase in nutrient availability. Therefore, the addition of vinasse can be an important strategy in maintaining and increasing long-term soil fertility in sugarcane cropping systems. environment However technologies preharvest pre harvest texture Design conducted treatments replications evaluated T irrigation 0 ( 2 4 6 determined p≤0.05 p005 p 05 (p≤0.05 probability 77 7 7. beta porosity saturation nutrients Therefore longterm long term systems p≤0.0 p00 (p≤0.0 p≤0. p0 (p≤0. p≤0 (p≤0 p≤ (p≤ (p

Abstract This study aims to investigate the gene expression of sperm-borne phospholipase C zeta (PLCζ), WW domain-binding protein 2N-Terminal Like (WBP2NL), and Tumor necrosis factor (TNF-α), as a negative control, in spermatozoa and their relationship with fertility and seminal quality in stallions. Ejaculates from 40 Criollo stallions were used, whose fertility was assessed on the basis of their pregnancy rate per cycle in at least two breeding seasons. Pregnancy rates ranged from 20% to 90% and were used to divide the stallions into two groups: High rates (≥ 50%) (n = 25), and Low rates (< 50%) (n = 15). A computer-assisted sperm analysis system - (CASA) analyzed semen after collection. Also were evaluated the physical and functional integrity of the plasmatic membrane and sperm morphology alterations. All stallions expressed PLCζ, WBP2NL, and TNF-α. PLCζ positively correlates with conception rate, total motility (TM), progressive motility (PM), plasmatic membrane functionality, and integrity. A simple linear regression was detected between pregnancy rate and PLCζ expression (P = 0.003), TM (P < 0.001) and PM (P < 0.001). PLCζ gene expression was higher (P = 0,012) in the High rates group than in the Low group. WBP2NL and TNF-α did not correlate with seminal quality and stallion’s fertility. It was concluded that PLCζ gene expression in the spermatozoa might be used as a biomarker of fertility and seminal quality in stallions. Parameters of sperm kinetics also showed, positive correlation between TM, PM and pregnancy rate. spermborne borne , (PLCζ) domainbinding domain binding 2NTerminal NTerminal 2N Terminal N WBPNL WBP NL (WBP2NL) TNFα, TNFα TNF α (TNF-α) control 4 seasons 20 90 groups ≥ ( 50% 50 n 25, 25 25) 15. 15 . 15) computerassisted computer assisted CASA (CASA collection alterations TNFα. α. (TM) PM, (PM) functionality P 0.003, 0003 0.003 0 003 0.003) 0.001 0001 001 0.001. 0,012 0012 012 stallion s showed (PLCζ (WBP2NL (TNF-α 2 9 5 1 (TM (PM 000 0.00 00 0,01 01 0.0 0,0 0. 0,

Resumo O uso de fertilizantes afeta não apenas a fertilidade do solo e o rendimento das culturas, mas também altera significativamente a estrutura taxonômica da microbiocenose do solo. Aqui, com base em experimento de campo estacionário, estudou-se a influência do fertilizante organomineral (FOM), modificado pela bactéria Bacillus subtilis (H-13), em comparação com diferentes sistemas de fertilizantes (orgânico, mineral, organomineral) em (i) rendimento da colheita, (ii) propriedades físicas e químicas, e (iii) diversidade alfa e beta da comunidade microbiana Albic Retisol (Loamic, Aric, Cutanic, Differentic, Ochric). O experimento foi conduzido sob duas condições: com calagem (pHKCl - 5,9) e sem calagem (pHKCl - 5,1). O uso apenas de esterco bovino foi menos eficaz do que sua coaplicação com fertilizantes minerais em metade das doses. Um efeito semelhante foi obtido ao aplicar FOM. Além disso, o uso de FOM contribui para um aumento significativo nas reservas de carbono orgânico do solo na camada de solo de 0-20 cm em 18%-32%. O sequenciamento de alto rendimento das bibliotecas de sequências do gene variável V4 do rRNA 16S identificou 10.759 táxons pertencentes a 456 gêneros, classificados em 34 filos (31 bacterianos e 3 arqueanos). A aplicação unilateral de fertilizantes minerais levou a uma diminuição significativa da diversidade alfa da estrutura das comunidades microbianas do solo (OTE [outras coisas iguais] e índice de Shannon). Foi observada uma aglutinação da microbiota nas variantes com e sem a aplicação de esterco bovino. O estudo revelou que a estrutura taxonômica da microbiocenose é formada sob a influência de dois fatores principais: cultura de rotação de culturas e fertilizantes aplicados. O tipo de cultura cultivada determina a dinâmica do microbiota ao nível de taxa maiores, como os domínios, e os fertilizantes afetam a estrutura da comunidade microbiana a um nível taxonômico inferior (filos, ordens, famílias). Com base na análise Deseq, foram identificados taxa marcadores, de acordo com a participação dos quais é possível determinar o tipo de cultura cultivada e os fertilizantes utilizados na experiência. A compreensão da dinâmica da associação de taxa e de outros fatores influentes pode levar à criação de sistemas universais de indicaçadores metagenômicos, onde o rastreio da dinâmica das comunidades microbianas permitirá uma avaliação abrangente do estado agroecológico dos solos e decisões oportunas para prevenir a sua degradação. Aqui estacionário estudouse estudou se FOM, , (FOM) H13, H13 H 13 (H-13) orgânico, (orgânico mineral i (i colheita ii (ii químicas iii (iii Loamic, Loamic (Loamic Aric Cutanic Differentic Ochric. Ochric . Ochric) condições pHKCl 5,9 59 5 9 5,1. 51 5,1 1 5,1) doses disso 020 0 20 0-2 18%32%. 1832 18% 32%. 18 32 18%-32% V S 10759 10 759 10.75 45 gêneros 31 (3 arqueanos. arqueanos arqueanos) OTE outras iguais Shannon. Shannon Shannon) principais aplicados maiores domínios filos, (filos ordens famílias. famílias famílias) Deseq marcadores experiência metagenômicos degradação (FOM H1 (H-13 5, 02 2 0- 18%32% 183 32% 18%-32 1075 75 10.7 4 ( (H-1 18%32 18%-3 107 7 10. (H- 18%3 18%- (H

Abstract The use of fertilizers affects not only the soil fertility and crop yield, but also significantly changes the taxonomic structure of the soil microbiocenosis. Here, based on stationary field experiment, we studied the influence of organo-mineral fertilizer (ОМF), modified by bacteria Bacillus subtilis, H-13 in comparison with different fertilizer systems (organic, mineral, organo-mineral) on (i) crop yield, (ii) physical and chemical properties, and (iii) alpha and beta diversity of the microbial community Albic Retisol (Loamic, Aric, Cutanic, Differentic, Ochric). The studies were carried out against the background of liming (рНКCl – 5.9) and without it (рНКCl – 5.1). The use of only one cattle farmyard manure was less effective than its co-application with mineral fertilizers in half doses. A similar effect was obtained when applying ОМF. In addition, the use of OMF contributes to a significant increase in the reserves of soil organic carbon in the soil layer 0-20 cm by 18%-32%. Using high-throughput sequencing of the 16S rRNA variable V4 gene sequence libraries, 10.759 taxa from 456 genera were identified, assigned to 34 fila (31 bacterial and 3 archaeotic. Unilateral application of mineral fertilizers leads to a significant decrease in the alpha diversity of the structure of soil microbial communities (OTE (other things equal) and Shannon index). A clear clustering of the microbiota was found in the variants with and without the introduction of сattle farmyard manure. It is revealed that the taxonomic structure of the microbiocenosis is formed under the influence of two main factors: crop rotation culture and applied fertilizers. The type of cultivated crop determines the dynamics of the microbiota at the level of larger taxa, such as domains, and fertilizers affect the structure of the microbial community at alower taxonomic level (phyla, orders, bloodlines). On the basis of the Deseq analysis, marker taxa were identified, according to the share participation of which it is possible to determine the type of cultivated crop and fertilizers used in the experiment. Understanding the dynamics of taxa association and other influential factors can lead to the creation of universal systems of metagenomic indication, where tracking the dynamics of microbial communities will allow for a comprehensive assessment of the agroecological state of soils and timely decisions to prevent their degradation. yield Here experiment organomineral organo ОМF, ОМF , (ОМF) subtilis H13 H 13 H-1 organic, (organic i (i ii (ii properties iii (iii Loamic, Loamic (Loamic Aric Cutanic Differentic Ochric. Ochric . Ochric) рНКCl 5.9 59 5 9 5.1. 51 5.1 1 5.1) coapplication co doses addition 020 0 20 0-2 18%32%. 1832 18% 32%. 18 32 18%-32% highthroughput high throughput S V libraries 10759 10 759 10.75 45 identified 31 (3 archaeotic OTE equal index. index index) domains phyla, phyla (phyla orders bloodlines. bloodlines bloodlines) analysis indication degradation (ОМF H1 H- 5. 02 2 0- 18%32% 183 32% 18%-32 1075 75 10.7 4 ( 18%32 18%-3 107 7 10. 18%3 18%-

ABSTRACT The significant role of Aedes aegypti as a vector of several arboviruses has intensified studies on vector control tools, including Attractive Toxic Sugar Baits containing Pyriproxyfen (ATSB-PPF). PPF is an insect growth regulator (IGR) that has been used to control immature mosquitoes, but it also has direct effects on female reproduction. This study evaluated the effect of physical exposure of Ae. aegypti females with ATSB-PPF on fecundity and fertility at different times before and after blood meal. Females were confined in cages impregnated with ATSB-PPF at different concentrations (5, 50, and 500 ppm). One group of females was exposed to the baits 24 and 48 hours Before the Blood Meal (BBM), and in the other group, exposure occurred 24 and 48 hours After the Blood Meal (ABM). The number of eggs laid, larvae, and pupation rate were analyzed. Exposure of Ae. aegypti females to ATSB-PPF bait 24 hours after the blood meal was able to reduce the number of eggs laid, especially at the higher concentrations used, but the number of larvae obtained was reduced regardless of the exposure time to ATSB-PPF, i.e., 24 hours before or after the blood meal, or the concentration of PPF used. No changes were observed in the number of pupae from larvae obtained from eggs laid by mosquitoes exposed to ATSB-PPF. Physical exposure of mosquitoes to sugar baits is sufficient to reduce the investigated reproductive parameters of Ae. aegypti. tools ATSBPPF. ATSBPPF ATSB . (ATSB-PPF) IGR (IGR reproduction Ae 5, 5 (5 50 ppm. ppm ppm) 2 4 BBM, BBM , (BBM) ABM. ABM (ABM) analyzed ATSBPPF, PPF, ie i e i.e. PPF. (ATSB-PPF ( (BBM (ABM i.e

ABSTRACT Most studies concerning biochar, which can enhance chemical, physical, and biological soil qualities and increase crop yields, are short-term experiments in controlled environments with temperate regions and sandy soils. Biochar amendment benefits for clayey soils in tropical climate regions are uncertain. Herein, field conditions in clayey soil were investigated for 28 months. Eucalyptus biochar was obtained from charcoal production and incorporated in soil with disc plows (0.00–0.20 m). Treatments comprised six single biochar application amounts–0 (control), 10, 20, 30, 40, and 50 Mg·ha-1. The experiments were conducted in April 2016, and corn was cultivated in 2016/17 and 2017/18. Soil samples were collected in 2018 for macro/micronutrient, bulk density, porosity, macroporosity, microporosity, water availability, and soil penetration resistance evaluations in the 0.00–0.05-, 0.05–0.10-, 0.10–0.20-, 0.20–0.30-, and 0.30–0.40-m layers. Chemicals P, K and Ca increased compared to the control. The K and Ca increases were related to the biochar composition (6% K and 2% Ca). Soil bulk density decreased by 0.07 kg·dm-3, and macro/microporosity (total porosity) increased linearly by 2.5% in the 0.05–0.10-m layer with increasing biochar application amount. Soil penetration resistance was unaffected. For each 1 Mg·ha-1 of biochar applied, a 500-L·ha-1 increase in available water occurred, which may be important for crops with low water demand and to overcome water restriction periods. chemical physical yields shortterm short term uncertain Herein 2 months 0.00–0.20 000020 0 00 20 (0.00–0.2 m. m . m) amounts0 amounts amounts– control, control , (control) 10 30 40 5 Mg·ha1. Mgha1 Mgha Mg·ha 1. Mg ha 2016 201617 17 2016/1 201718 2017 18 2017/18 201 macromicronutrient macro micronutrient macro/micronutrient porosity macroporosity microporosity availability 0.00–0.05, 000005 0.00–0.05 05 0.00–0.05- 0.05–0.10, 005010 0.05–0.10 0.05–0.10- 0.10–0.20, 010020 0.10–0.20 0.10–0.20- 0.20–0.30, 020030 0.20–0.30 0.20–0.30- 0.30–0.40m 030040m 0.30–0.40 layers P 6% 6 (6 Ca. Ca) 007 07 0.0 kg·dm3, kgdm3 kgdm kg·dm 3, kg dm 3 kg·dm-3 macromicroporosity total 25 2.5 0.05–0.10m 005010m amount unaffected Mg·ha1 Mg·ha- applied 500L·ha1 500Lha1 Lha 500 L·ha L 500-L·ha- occurred periods 0.00–0.2 00002 (0.00–0. (control 4 ha1 20161 2016/ 20171 2017/1 00000 0.00–0.0 00501 0.05–0.1 01002 0.10–0.2 02003 0.20–0.3 40m 030040 0.30–0.4 ( 0. dm3 kg·dm3 kg·dm- 2. 10m 500L 500L·ha 500Lha 500-L·ha 0.00–0. 0000 (0.00–0 2017/ 0050 0.05–0. 0100 0.10–0. 0200 0.20–0. 03004 0.30–0. 0.00–0 000 (0.00– 005 0.05–0 010 0.10–0 020 0.20–0 0300 0.30–0 0.00– (0.00 0.05– 01 0.10– 02 0.20– 030 0.30– 0.00 (0.0 0.05 0.10 0.20 03 0.30 (0. 0.1 0.2 0.3 (0

ABSTRACT Shaded coffee systems may offer a series of benefits, however, studies on shaded cultivation of Conilon coffee crops are still scarce in the literature. This study aimed to evaluate the soil physical, chemical, and biological properties of two Conilon coffee intercropping systems from southeastern Brazil. Two commercial coffee crops – one shaded by macadamia trees and the other by green dwarf coconut trees – were evaluated according to three treatments: coffee plants in the inter-row of the tree species; coffee plants in the same row of the trees; and unshaded coffee. The experimental design was a completely randomized with four replicates. Coffee plants intercropped with macadamia trees, both intra- and inter-row, resulted in less soil compaction than unshaded systems. As for intercropping with green dwarf coconut trees, the unshaded system presented lower soil resistance to penetration. Differences in physical properties between treatments allow no inferences about intercropping systems influence on green coconut trees. Intercropped coffee improves soil chemical properties, resulting in greater soil fertility than unshaded systems, and showed greater soil organisms. These findings indicate that Conilon coffee-macadamia intercropped with tree species represents a promising alternative for sustainable soil management. benefits however literature Brazil interrow inter replicates intra interrow, row, penetration organisms coffeemacadamia management

Abstract Leaf litter is an important component related to nutrient cycling and conservation of soil biodiversity. In this context, the objective of our study was to investigate the relationship between soil and leaf litter in different forest communities in sub-regions of the Pantanal (Nhecolândia and Abobral/Miranda). To this end, we collected soil and leaf litter samples from 152 sample plots divided in physiognomies regionally called as Cordilheira, Capão, Murundu, Corixo and Cambará/Canjiqueira forest. In the laboratory, analytical determinations were conducted for 22 soil attributes and 11 elements of the chemical composition of the leaf litter. The forest communities showed a great well-defined edaphic gradient encompassing environments with high natural fertility soils (Capão and Cordilheira Abobral/Miranda), acidic soils with a high proportion of soluble Al3+ (Cambará/Canjiqueira, and Corixo), and poorly developed soils with a predominantly sandy texture (Nhecolândia region, Cordilheira and Murundu). Soil attributes are significantly related to the chemical composition of litter, as indicated by correlations between N, P, Ca, Mg, Mn and the C/N ratio with physical, chemical and soil organic matter attributes. This study revealed the interaction of soils with the chemical composition of litter, constituting an ecosystem service, in forest communities in the Brazilian Pantanal. biodiversity context subregions sub regions Nhecolândia Abobral/Miranda. AbobralMiranda Abobral/Miranda . Abobral Miranda Abobral/Miranda) end 15 Capão Murundu CambaráCanjiqueira Cambará Canjiqueira laboratory 2 1 welldefined well defined Abobral/Miranda, , Al3 Al Cambará/Canjiqueira, (Cambará/Canjiqueira Corixo, Corixo) region Murundu. Murundu) N P Ca Mg CN C physical service

Resumen Introducción: Las áreas impactadas por minería en bosques tropicales requieren de la aplicación de estrategias de restauración ecológica, pero este proceso, muchas veces involucra el uso de especies vegetales exóticas, desconociendo los efectos sobre la regeneración ecológica de los sitios donde se introducen. Objetivo: Evaluar el efecto de las plantaciones de Acacia mangium (planta exótica) sobre la rehabilitación ecológica temprana (suelo y vegetación) de áreas impactadas por minería de oro a cielo abierto en la selva pluvial tropical del Chocó, Colombia. Métodos: Se seleccionaron 16 áreas mineras como unidades de muestreo (ocho reforestadas con A. mangium y ocho en sucesión natural) en dos localidades. En cada unidad de muestreo se estableció una parcela de 2 × 50 m (cuatro parcelas por escenario de muestreo y localidad), donde se analizó la fertilidad del suelo (parámetros físicos y químicos) y se cuantificó el número de individuos de cada especie de planta vascular. Resultados: Se registraron 73 especies (69 géneros, 45 familias). La densidad de individuos fue mayor en áreas de sucesión natural que en aquellas reforestadas con A. mangium; por el contrario, la riqueza y diversidad de especies fueron superiores bajo las plantaciones de A. mangium. La similitud florística fue baja entre escenarios sucesionales (especies compartidas 35.6 %). El suelo mostró mejores condiciones (especialmente, N-NHO3) en áreas con A. mangium que en áreas en regeneración natural. Conclusiones: Las plantaciones de A. mangium parecen facilitar la rehabilitación temprana de la fertilidad del suelo y la vegetación en las minas abandonadas; por lo tanto, esta especie puede jugar un papel importante para la implementación de estrategias de restauración ecológica de áreas impactadas por minería de oro a cielo abierto en el Chocó y otros sistemas forestales tropicales con condiciones ambientales y de perturbación similares.

Abstract Introduction: Mining-impacted areas in tropical forests require the application of ecological restoration strategies, but this process often involves use of exotic plant species ignoring the effects on the ecological regeneration of the sites where they are introduced. Objective: To evaluate the effect of Acacia mangium plantations (exotic plant) on early ecological rehabilitation (soil and vegetation) of areas impacted by open-pit gold mining in the tropical rain forest of Chocó, Colombia. Methods: 16 mining areas were selected as sampling units (eight reforested with A. mangium and eight in natural succession) in two locations. In each sampling unit, a 2 × 50 m plot was established (four plots per sampling scenario and locality), where soil fertility (physical and chemical parameters) was analyzed and the number of individuals of each vascular plant species was quantified. Results: 73 species (69 genera, 45 families) were recorded. The density of individuals was higher in areas of natural succession than in those reforested with A. mangium; conversely, species richness and diversity were higher under the A. mangium plantations. Floristic similarity was low between successional scenarios (shared species 35.6 %). The soil showed better conditions (especially N-NHO3) in mining areas with A. mangium than in those in natural regeneration. Conclusions: A. mangium plantations appears to facilitate the early rehabilitation of soil fertility and vegetation in abandoned mines; therefore, this species can play an important role in the implementation of ecological restoration strategies in areas impacted by open-pit gold mining in the Chocó and other tropical forest systems with similar environmental and disturbance conditions.

Resumen Una estrategia para evaluar suelos es mediante índices de calidad que dependen de indicadores específicos sobre los suelos muestreados, el tipo de cultivo y el manejo realizado. Los indicadores son variables físicas, químicas o biológicas medibles, que afectan la capacidad del suelo al ejercer una o varias de sus funciones. El objetivo de esta investigación fue examinar el uso metodológico de las diferentes propiedades físicas, químicas y biológicas del suelo usadas como indicadores de calidad para determinar índices de calidad del suelo en México, a través de una revisión bibliográfica realizada en el año 2022, para el período 2000-2021 mediante diversos buscadores de artículos científicos y palabras clave relacionadas al tema, con la finalidad de generar un diagnóstico y vislumbrar oportunidades de investigación. Se debe prestar mayor atención al estudio de la calidad de suelos en México, con base en información técnica y científica en regiones y estados dónde esta información siga siendo escasa. Producto de la revisión bibliográfica se propone como un conjunto de indicadores físicos: a la textura, densidad aparente, estabilidad de agregados, infiltración, resistencia a la penetración, curva de retención de humedad y profundidad del suelo; como indicadores químicos: materia orgánica, pH, nitrógeno total, nitrógeno inorgánico, fósforo, potasio, calcio y magnesio y como indicadores biológicos: carbono en la biomasa microbiana, respiración del suelo, densidad de lombrices, deshidrogenasa, β-glucosidasa, ureasa, fosfatasa y a los hongos micorrízicos arbusculares, optando por un subconjunto de indicadores o por un conjunto mínimo de datos para conformar un índice de calidad de suelo.

Abstract One strategy for evaluating soils is through quality indices that depend on specific indicators on the soils sampled, the type of crop and the management carried out. Indicators are measurable physical, chemical or biological variables that affect the capacity of the soil as they perform one or more of its functions. The objective of this research was to examine the methodological use of the different physical, chemical and biological properties of the soil used as quality indicators to determine soil quality indices in Mexico, through a bibliographic review carried out in 2022, for the period 2000-2021 through various search engines of scientific articles and keywords related to the subject, in order to generate a diagnosis and glimpse research opportunities. Greater attention should be paid to the study of soil quality in Mexico, based on technical and scientific information in regions and states where this information remains scarce. As a product of the bibliographic review, the following sets of indicators are proposed: texture, bulk density, aggregate stability, infiltration, penetration resistance, moisture retention curve and soil depth as physical indicators; organic matter, pH, total nitrogen, inorganic nitrogen, phosphorus, potassium, calcium and magnesium as chemical indicators; and microbial biomass carbon, soil respiration, earthworm density, dehydrogenase, β-glucosidase, urease, phosphatase and arbuscular mycorrhizal fungi as biological indicators, opting for a subset of indicators or a minimum data set to form a soil quality index.