Abstract Introduction: In Colombia, Aedes aegypti is present in 80 % of the country and little is known about the kdr mutations related to pyrethroids (PY) resistance, as well as the influence of different biomes (mainly temperature) and passive ground delivery transport on maintaining and disseminating populations with these mutations. Objective: To model the behavior of genotypic frequencies associated with the kdr Val1016Ile + Phe1534Cys mutation in A. aegypti populations, considering vector population movement between Colombian biomes through passive ground delivery transport. Method: We obtained data from the literature regarding population dynamics, mosquito life cycle parameters associated with temperatures for each Colombian biome, and ground cargo transportation, including truck transport. We also evaluated the impact of the evolutionary cost for the kdr Val1016Ile and Phe1534Cys mutations on oviposition and death rates. Results: Population behavior is influenced by the evolutionary cost of resistant genotypes and passive cargo transport. Once resistant genotypes arrive in a biome through truck-mediated mosquito transport, they persist. Conclusion: Passive migration through land cargo transport is essential for the dissemination of individual resistance carriers between different regions or biomes. Evolutionary cost plays a critical role in the dynamics of kdr mutations in A. aegypti populations.

Resumen Introducción: En Colombia, Aedes aegypti se encuentra presente en el 80 % del país y poco se conoce sobre las mutaciones kdr que están relacionadas con la resistencia a piretroides (PY), así como la influencia de los diferentes biomas (principalmente la temperatura) y el transporte pasivo de carga terrestre, en el mantenimiento y diseminación de poblaciones que presenten estas mutaciones. Objetivo: Modelar el comportamiento de las frecuencias genotípicas asociadas a la mutación kdr Val1016Ile + Phe1534Cys, en poblaciones de A. aegypti, considerando el movimiento de las poblaciones del vector entre biomas colombianos a través del transporte pasivo por carga terrestre. Método: Se obtuvieron datos de la literatura asociados con la dinámica poblacional y los parámetros del ciclo de vida del mosquito asociados con las temperaturas de cada bioma colombiano y el transporte de carga terrestre a través de camiones entre los biomas. Además, se evaluó el impacto del costo evolutivo para las mutaciones kdr Val1016Ile y Phe1534Cys representado en las tasas de ovoposición y muerte. Resultados: El comportamiento de las poblaciones está influenciado tanto por el costo evolutivo de los genotipos resistentes como por el transporte pasivo de carga y, que una vez los genotipos resistentes lleguen a un bioma gracias al transporte de mosquitos mediado por camiones, estos se mantienen allí. Conclusión: La migración pasiva a través del transporte de carga terrestre, como medio de flujo genético de individuos portadores de resistencia a PY entre diferentes regiones o biomas, se revela como un factor esencial para la diseminación, y el costo evolutivo como un factor determinante en la dinámica, de las mutaciones kdr en las poblaciones de A. aegypti.

ABSTRACT Chinch bugs [Blissus pulchellus Montandon (Hemiptera: Blissidae)] suck the phloem from susceptible forage grasses, injecting toxins that destroy plant vessels and compromise the flow of water and nutrients, leading to plant death. Thus, the aim of this study was to assess eight forage grasses for antixenosis resistance to B. pulchellus and compare the anatomical characteristics of leaf sheath tissue from resistant and susceptible species/cultivars. Experiments were conducted in a laboratory and greenhouse, using choice and no-choice tests with the following forage grasses: Urochloa ruziziensis, U. humidicola, U. brizantha ‘Piatã’, U. brizantha ‘Paiaguás’, U. brizantha ‘Marandu’, Panicum maximum ‘Mombaça’, P. maximum ‘Zuri’, and Andropogon gayanus. The oviposition results demonstrated that in the choice test there was a change in the stink bug’s behavior in relation to grasses four hours after infestation, with U. humidicola, P. maximum ‘Mombaça’ and ‘Zuri’, U. brizantha ‘Marandu’ and A. gayanus, proving to be less attractive to insects. It is concluded that U. humidicola, P. maximum ‘Mombaça’ and ‘Zuri’, U. brizantha ‘Marandu’, and A. gayanus are less attractive to B. pulchellus. A. gayanus and P. maximum ‘Mombaça’ and ‘Zuri’ showed non-preference resistance (antixenosis) to oviposition by B. pulchellus. The resistance of U. humidicola, P. maximum ‘Zuri’, and A. gayanus to B. pulchellus may be associated with greater compaction and lignification of the parenchyma and sclerenchyma cells of leaf sheaths. Blissus Hemiptera (Hemiptera Blissidae Blissidae) nutrients death Thus B speciescultivars species cultivars species/cultivars greenhouse nochoice no ruziziensis U humidicola ‘Piatã, Piatã ‘Piatã , ‘Piatã’ ‘Paiaguás, Paiaguás ‘Paiaguás ‘Paiaguás’ ‘Marandu, Marandu ‘Marandu ‘Mombaça, Mombaça ‘Mombaça P ‘Zuri, Zuri ‘Zuri bug s infestation A insects nonpreference non preference (antixenosis sheaths

RESUMO Os percevejos-das-gramíneas [Blissus pulchellus Montandon (Hemiptera: Blissidae)] sugam a seiva de espécies forrageiras suscetíveis e injetam toxinas que destroem os vasos condutores e dificultam o transporte de água e nutrientes, levando as plantas a morte. Assim, o objetivo deste estudo foi avaliar a resistência por antixenose e tolerância em oito poáceas forrageiras à B. pulchellus e comparar as características anatômicas dos tecidos das bainhas foliares de espécies/cultivares resistentes e suscetíveis. Os ensaios foram conduzidos em laboratório e casa de vegetação, em testes com e sem chance de escolha, utilizando-se as forrageiras: Urochloa ruziziensis, U. humidicola, U. brizantha ‘Piatã’, U. brizantha ‘Paiaguás’, U. brizantha ‘Marandu’, Panicum maximum ‘Mombaça’, P. maximum ‘Zuri’, e Andropogon gayanus. Os resultados de oviposição demonstraram que no teste de escolha houve uma mudança no comportamento do percevejo em relação às gramíneas quatro horas após infestação, sendo U. humidicola, P. maximum ‘Mombaça’ e ‘Zuri’, U. brizantha ‘Marandu’ e A. gayanus menos atrativos para os insetos. Conclui-se que U. humidicola, P. maximum ‘Mombaça’ e ‘Zuri’, U. brizantha ‘Marandu’, e A. gayanus são menos atrativas a B. pulchellus. A. gayanus, e P. maximum ‘Mombaça’ e ‘Zuri’ apresentam resistência do tipo não-preferência para oviposição a B. pulchellus. A resistência de U. humidicola, P. maximum Zuri, e A. gayanus a B. pulchellus pode estar associada a maior compactação e lignificação das células dos tecidos parenquimático e esclerenquimático das bainhas foliares. percevejosdasgramíneas percevejos Blissus Hemiptera (Hemiptera Blissidae Blissidae) nutrientes morte Assim B espéciescultivares cultivares vegetação utilizandose utilizando se ruziziensis U humidicola ‘Piatã, Piatã ‘Piatã , ‘Piatã’ ‘Paiaguás, Paiaguás ‘Paiaguás ‘Paiaguás’ ‘Marandu, Marandu ‘Marandu ‘Mombaça, Mombaça ‘Mombaça P ‘Zuri, Zuri ‘Zuri infestação insetos Concluise Conclui nãopreferência não preferência

Abstract Objectives : To monitor the oviposition activity of the mosquito Aedes aegypti and of dengue and chikungunya cases in four localities of temperate Argentina, during the 2023 epidemic. Methods : During the summer and autumn of 2023, the oviposition activity of the mosquito vector was monitored weekly using ovitraps, and the arrival of cases with dengue or chikungunya in Tandil, Olavarría, Bahía Blanca and Laprida were registered. Results : Monthly variations of the percentage of positive traps were similar in the first three locations; in Laprida the mosquito was not detected. On the con trary, a significant difference was observed in the per centage of total traps that ever tested positive in each locality, being higher in Olavarría (83.3%) than in Bahía Blanca (68.6%) and Tandil (48.7%). Regarding diseases, 18 imported cases of dengue and 3 of chikungunya were registered. In addition, the first autochthonous case of dengue in the region was recorded, being the southern most until known. Conclusion : It is essential to raise awareness and train the members of the health systems of the new regions exposed to Ae. aegypti for early detection of cases, and to the general population to enhance pre vention actions.

Resumen Objetivos : Monitorear la actividad de oviposición del mosquito Aedes aegypti y de casos de dengue y chikun gunya en cuatro localidades de Argentina templada, durante la epidemia del 2023. Métodos : Durante el verano y otoño del 2023, se mo nitoreó semanalmente mediante ovitrampas la actividad de oviposición del mosquito vector, y se registró el arribo de casos con dengue o chikungunya a Tandil, Olavarría, Bahía Blanca y Laprida. Resultados : La variación mensual del porcentaje de trampas positivas fue similar en las tres primeras localidades; en Laprida no se detectó el mosquito. Por el contrario, se observó una diferencia significativa del porcentaje de trampas que alguna vez resultó positiva en cada localidad, siendo mayor en Olavarría (83%), que en Bahía Blanca (67%) y Tandil (49%). Respecto a las enfermedades, se registraron 18 casos importados de dengue y 3 de chikungunya. Además, se registró el primer caso autóctono de dengue en la región, siendo el más austral hasta el momento. Conclusión : Es imprescindible sensibilizar y capacitar a los integrantes de los sistemas de salud de las nue vas regiones expuestas al Ae. aegypti para la detección temprana de casos, y a la población en general para potenciar las acciones de prevención.

ABSTRACT Plutella xylostella is one of the most important pests of brassicaceous crops around the world. Indoxacarb is widely used to control lepidopteran pest larvae. This insecticide has slow activity and prolonged residual effects which impact the habits and physiology of pests. The objective was to evaluate the effect of sublethal concentrations of indoxacarb on the biology and development time of the diamondback moth. Selection pressure of the insecticide indoxacarb was carried out on P. xylostella, applying the LC20 of the previous generation for the next ten generations, the LC20 of the resistant line (G10) was calculated and this concentration was subsequently applied to L3 larvae. The effect on the percentage and weight of pupae, adult emergence and oviposition for two generations was evaluated; in addition, the development time of egg, larva, pupa and adult. The results showed that resistance and exposure to LC20 of indoxacarb caused changes in the biology and development of P. xyllostella. This was manifested in an increase in the percentage and weight of formed pupae and emergence of adults, along with a prolongation of development time of more than 10 days. However, a decrease of more than 50% in the number of eggs per female was observed.

RESUMEN Plutella xylostella es una plaga de las más importantes en los cultivos de brassicaceas alrededor del mundo. El indoxacarb se utiliza ampliamente para el control de larvas de lepidópteros plaga. Este insecticida presenta una actividad lenta y efectos residuales prolongados los cuales impactan los hábitos y la fisiología de las plagas. Como objetivo se estableció la evaluación del efecto de concentraciones subletales de indoxacarb en la biología y tiempo de desarrollo de la palomilla dorso de diamante. Se realizó presión de selección del insecticida indoxacarb sobre P. xylostella, aplicando la CL20 de la generación anterior por las siguientes diez generaciones, se calculó la CL20 de la línea resistente (G10) y posteriormente se aplicó esta concentración a larvas de L3. Se evaluó el efecto en el porcentaje y peso de pupas, emergencia de adultos y oviposición para dos generaciones; además, del tiempo de desarrollo de huevo, larva, pupa y adulto. Los resultados mostraron que la resistencia y la exposición a CL20 de indoxacarb provocaron cambios en la biología y el desarrollo de P. xyllostella. Esto se manifestó en un aumento del porcentaje y peso de pupas formadas y emergencia de adultos, junto con una prolongación del tiempo de desarrollo de más de 10 días. Sin embargo, se observó una disminución de más del 50% en el número de huevos por hembra.

ABSTRACT The impact of transporting quail of four ages (0, 14, 28 and 42 days) from the coast to the Peruvian mountains on the onset of oviposition and performance in the laying phase was measured. The quails were moved from Lurin (Lima, Peru) at 14 meters above sea level to the Cajamarca valley at 2743 meters above sea level. A batch of 800 recently hatched female chicks in Lurin to distribute them in four groups of 200 birds each. Three groups were installed in Lurin and one group was taken to Cajamarca on the same day of birth. The other groups were transferred at 14, 28 and 42 days of age to Cajamarca. At 42 days of age, the birds were distributed per treatment at a rate of 15 birds per cage (one cage = one repetition). Each treatment had six repetitions. Quails transferred at 14, 28 and 42 days of age reached higher body weights and egg weights at the beginning of laying, with greater egg mass and better laying rate during the productive phase. Quails transferred at 28 and 42 days of age began laying at a younger age and with better feed conversion at laying.

RESUMEN Se midió el impacto del transporte de la codorniz de cuatro edades (0, 14, 28 y 42 días) desde la costa a la sierra peruana sobre el inicio de la oviposición y rendimiento en la fase de puesta. Las codornices fueron trasladadas desde Lurín (Lima, Perú) ubicado a 14 msnm hacia el valle de Cajamarca a 2743 msnm. Se trabajó con 800 cotupollos hembras recién eclosionadas en Lurín y se formaron cuatro grupos de 200 aves cada uno. Tres grupos fueron instalados en Lurín y un grupo fue llevado a Cajamarca el mismo día del nacimiento. Los otros grupos fueron trasladados a los 14, 28 y 42 días de edad a Cajamarca. A los 42 días de edad, las aves fueron distribuidas por tratamiento a razón de 15 aves por jaula (una jaula = una repetición). Cada tratamiento tuvo seis repeticiones. Las codornices trasladadas a los 14, 28 y 42 días de edad alcanzaron mayores pesos corporales y pesos de huevo al inicio de la puesta, con mayor masa de huevo y mejor tasa de puesta durante la fase productiva. Las codornices trasladadas a los 28 y 42 días de edad iniciaron la puesta a menor edad y con mejor conversión alimenticia en la puesta.

Abstract To determine the species composition, relative abundance and seasonal variation of different mosquitoes Genera (Aedes, Anopheles, Armigeres, Culex, and Culiseta) in different habitats the present research work was carried out in Entomology Research Laboratory of The University of Peshawar. Sampling performed from variety of permanent and temporary breeding habitats was carried out on monthly basis from targeted breeding sites for two consecutive years through dipping method. Species diversity in the survey sites was noted. Collection from these seventeen various types of potential larval habitats, yielded a total of 42,430 immature constituting 41,556 larvae and 874 pupae. Among these only 19,651 adult mosquitoes emerged comprising 11,512 female and 8,139 male mosquitoes. 78% (n= 15333) of mosquito larvae were from permanent and 22% (n=4318) were from temporary breeding sites. This study showed that Peshawar valley harbours 15 species from the genera Aedes, Anopheles, Armigeres, Culex and Culiseta. When the density of each species was examined, Culex quinquifasciatus was found to be dominant (79%) and constant in distribution. Among the temporary habitats Aedes albopictus was found as the most prevalent species particularly from tree holes and water cisterns. The highest intensity of mosquitoes was in June (2243 emerged adults) and November (2667 emerged adults) while the lowest was in January (203 emerged adults). A perfect positive correlation (r = +0.8) was found between temperature and population of mosquitoes (df 10 and α 0.05). The species diversity index for mosquitoes remained between 0.12 and 1.76. The Margalef’s richness components was noticeably low for bamboo traps (0.2) and fairly high for rice fields, Percolating water and Animal tracks (1.3) which shows the abundance of mosquito species in these habitats. Similarly Pielou’s Evenness was highest for bamboo traps (E=1) showing species uniform distribution. Animal tracks were presumed not only the diverse habitat rather also possessed high value for species richness and species evenness. Temperature, rainfall, humidity and other related attributes responsible for species variation and abundance need to be analysed further to pave way for controlling vector species in their oviposition targeted sites. composition (Aedes Anopheles Armigeres Culiseta method noted 42430 42 430 42,43 41556 41 556 41,55 87 pupae 19651 19 651 19,65 11512 11 512 11,51 8139 8 139 8,13 78 n= n (n 15333 22 n=4318 n4318 4318 (n=4318 1 examined 79% 79 (79% distribution cisterns 2243 (224 adults 2667 (266 203 (20 adults. . r +0.8 08 0 df 0.05. 005 0.05 05 0.05) 012 12 0.1 176 76 1.76 Margalefs Margalef s 0.2 02 2 (0.2 fields 1.3 13 3 (1.3 Pielous Pielou E=1 E1 E (E=1 evenness Temperature rainfall 4243 4 43 42,4 4155 55 41,5 1965 65 19,6 1151 51 11,5 813 8,1 7 1533 n=431 n431 431 (n=431 (79 224 (22 266 (26 20 (2 +0. 00 0.0 01 0. 17 1.7 (0. 1. (1. E= (E= 424 42, 415 5 41, 196 6 19, 115 11, 81 8, 153 n=43 n43 (n=43 (7 26 ( +0 (0 (1 (E n=4 n4 (n=4 +

Resumo Para determinar a composição de espécies, abundância relativa e variação sazonal de diferentes gêneros de mosquitos (Aedes, Anopheles, Armigeres, Culex e Culiseta) em diferentes habitats, o presente trabalho foi realizado no Laboratório de Pesquisa em Entomologia da Universidade de Peshawar. A amostragem coletada a partir de uma variedade de habitats de reprodução permanentes e temporários foi realizada mensalmente a partir de locais de reprodução alvo por 2 anos consecutivos através do método de imersão. A diversidade de espécies nos locais de pesquisa foi anotada. A coleta desses 17 tipos diferentes de habitats larvais potenciais rendeu um total de 42.430 imaturos, constituindo 41.556 larvas e 874 pupas. Destes, emergiram apenas 19.651 mosquitos adultos, sendo 11.512 fêmeas e 8.139 machos. 78% (n = 15333) das larvas do mosquito eram de criadouros permanentes e 22% (n = 4318) de criadouros temporários. Este estudo mostrou que o vale de Peshawar abriga 15 espécies dos gêneros Aedes, Anopheles, Armigeres, Culex e Culiseta. Quando a densidade de cada espécie foi examinada, Culex quinquifasciatus foi considerado dominante (79%) e constante na distribuição. Entre os habitats temporários, o Aedes albopictus foi encontrado como a espécie mais prevalente, principalmente em ocos de árvores e cisternas de água. A maior intensidade de mosquitos foi em junho (2.243 adultos emergidos) e novembro (2.667 adultos emergidos), enquanto a menor foi em janeiro (203 adultos emergidos). Uma correlação positiva perfeita (r = +0,8) foi encontrada entre temperatura e população de mosquitos (DF 10 e α 0,05). O índice de diversidade de espécies de mosquitos permaneceu entre 0,12 e 1,76. Os componentes de riqueza de Margalef foram visivelmente baixos para armadilhas de bambu (0,2) e razoavelmente altos para campos de arroz, água percolada e rastros de animais (1,3), o que mostra a abundância de espécies de mosquitos nesses habitats. Da mesma forma, a uniformidade de Pielou foi maior para armadilhas de bambu (E = 1), mostrando distribuição uniforme das espécies. As pegadas de animais foram presumidas não apenas como habitat diverso, mas também possuíam alto valor para riqueza e uniformidade de espécies. Temperatura, chuva, umidade e outros atributos relacionados responsáveis pela variação e abundância das espécies precisam ser analisados mais a fundo para abrir caminho para o controle de espécies de vetores em seus locais de oviposição alvo. (Aedes Anopheles Armigeres Culiseta imersão anotada 1 42430 42 430 42.43 imaturos 41556 41 556 41.55 87 pupas Destes 19651 19 651 19.65 11512 11 512 11.51 8139 8 139 8.13 machos 78 n 15333 22 4318 examinada 79% 79 (79% prevalente 2.243 2243 243 (2.24 emergidos 2.667 2667 667 (2.66 emergidos, , 203 (20 emergidos. . r +0,8 08 0 DF 0,05. 005 0,05 05 0,05) 012 12 0,1 176 76 1,76 0,2 02 (0,2 arroz 1,3, 13 1,3 3 (1,3) forma E 1, 1) diverso Temperatura chuva 4243 4 43 42.4 4155 55 41.5 1965 65 19.6 1151 51 11.5 813 8.1 7 1533 431 (79 2.24 224 24 (2.2 2.66 266 66 (2.6 20 (2 +0, 00 0,0 01 0, 1,7 (0, (1,3 424 42. 415 5 41. 196 6 19. 115 11. 81 8. 153 (7 2.2 (2. 2.6 26 ( +0 (0 (1, 2. + (1

Abstract The pink sugarcane mealybug Saccharicoccus sacchari (Cockerell, 1895) (Hemiptera: Pseudococcidae) occurs practically in all sugarcane producing regions Saccharum spp. (Poaceae) causing damage. Little information is available about insect’s biology under Brazilian conditions. In this work, biological development of pink sugarcane mealybug was studied at temperatures of 23 °C ± 2 °C and 28 °C ± 2 °C without photophase and relative humidity of 80%. Number and viability of eggs, incubation time, duration of the last oviposition, duration of each nymphal instar, viability of the nymphs, start of oviposition and longevity of the females were recorded. Biological development of insects was stipulated by the SAS University Edition software, version 9.4. There were differences in the life cycle of the pseudococcid at both temperatures evaluated. Females of S. sacchari had three nymphal instars and reproduce asexually. Asexual reproduction occurs in the field and under controlled conditions. By increasing the temperature increases, insect lived longer and the presence of the winged male in Brazil indicates the possibility of sexual reproduction of the species. Cockerell, Cockerell (Cockerell 1895 Hemiptera (Hemiptera Pseudococcidae spp Poaceae (Poaceae damage s conditions work C 80 80% eggs time instar nymphs recorded software 94 9 4 9.4 evaluated S asexually increases species 189 8 9. 18 1

Resumo A cochonilha-rosada-da-cana-de-açúcar Saccharicoccus sacchari (Cockerell, 1895) (Hemiptera: Pseudococcidae) ocorre praticamente em todas as regiões produtoras de cana-de-açúcar Saccharum spp. (Poaceae) causando prejuízos. Poucas informações estão disponíveis sobre a biologia do inseto nas condições brasileiras. Neste trabalho foi estudado o desenvolvimento biológico da cochonilha-rosada-da-cana-de-açúcar nas temperaturas de 23 °C ± 2 °C e 28 °C ± 2 °C sem fotofase e umidade relativa de 80%. Diariamente foi registrado o número e viabilidade dos ovos, tempo de incubação, duração da última oviposição, duração de cada ínstar ninfal, viabilidade das ninfas, início da oviposição e longevidade das fêmeas. O desenvolvimento biológico dos insetos foi estipulado pelo software SAS University Edition, versão 9.4. Houve diferenças no ciclo de vida do pseudococcídeo nas duas temperaturas avaliadas. As fêmeas de S. sacchari apresentaram três instares ninfais e reproduzem assexuadamente. A reprodução assexuada ocorre em campo e em condições controladas. Ao se aumentar a temperatura o inseto viveu mais e a presença do macho alado no Brasil indica a possibilidade da reprodução sexuada da espécie. cochonilharosadadacanadeaçúcar cochonilha rosada cana açúcar Cockerell, Cockerell (Cockerell 1895 Hemiptera (Hemiptera Pseudococcidae canadeaçúcar spp Poaceae (Poaceae prejuízos brasileiras C 80 80% ovos incubação ninfal ninfas Edition 94 9 4 9.4 avaliadas S assexuadamente controladas espécie 189 8 9. 18 1

Resumo O ácaro da tamareira, Oligonychus afrasiaticus (McGregor) (Acari: Tetranychidae), é uma praga grave das tâmaras no Médio Oriente e Norte de África, causando graves danos econômicos se não for controlado precocemente. Como os ácaros predadores são conhecidos por serem potenciais agentes de biocontrole contra diversas pragas, também a capacidade de predação, tabela de vida, reprodução e sobrevivência de Amblyseius swirskii Athias-Henriot e Neoseiulus cucumeris (Oudemans) (Acari: Phytoseiidae), coletados em fazendas de tamareiras em Qassim Arábia Saudita, foram estudados em condições de laboratório (25 °C, 30 °C, 35 °C e 50 ± 5% UR) contra todos os estágios móveis de O. afrasiaticus. Para ambos os predadores, o tempo médio de desenvolvimento, o período de oviposição e a longevidade foram inversamente relacionados à temperatura de 25 °C a 35 °C. Vários parâmetros foram estudados para A. swirskii e N. cucumeris a 25 °C, 30 °C e 35 °C, ou seja, o tempo de desenvolvimento feminino, 9,37, 7,29, 5,56 e 10,67, 8,38, 6,45 d; período de oviposição, 19,77, 16,18, 13,94 e 15,90, 13,84, 10,64 d; longevidade, 29,39, 24,79, 20,64 e 25,42, 21,94, 17,39 dias; fecundidade, 31,91, 37,10, 42,16 e 21,75, 26,84, 30,56 ovos por fêmea, respectivamente. A taxa máxima de predação diária para ambos os predadores foi registrada a 35 °C durante o período de oviposição. A predação total de fêmeas de A. swirskii e N. cucumeris foi de 370,86, 387,54, 405,83, 232,14, 263,32, 248,85 presas a 25 °C, 30 °C e 35 °C respectivamente. A taxa máxima de reprodução de A. swirskii e N. cucumeris (3,02; 2,87 ovos/♀/dia) foi registrada a 35 °C, enquanto a mínima (2,00; 1,36 ovos/♀/dia) foi registrada a 25 °C. Os parâmetros da tabela de vida foram estimados como taxa reprodutiva líquida (Ro) 21,68, 25,94, 29,52 e 18,95, 20,25, 22,78; o tempo médio de geração (T) 24,92, 21,82, 18,24 e 26,30, 23,60, 20,56 d; a taxa intrínseca de aumento (rm) 0,181, 0,232, 0,248 e 0,170, 0,185, 0,196; a taxa finita de aumento (λ) 1,365, 1,551, 1,706 e 1,126, 1,324, 1,428 para A. swirskii e N. cucumeris a 25 °C, 30 °C e 35 °C respectivamente. Os resultados deste estudo sugerem que as duas espécies de fitoseídeos são promissores agentes de controle biológico de O. afrasiaticus em uma ampla faixa de temperaturas. tamareira McGregor (McGregor Acari (Acari Tetranychidae, Tetranychidae , Tetranychidae) África precocemente pragas AthiasHenriot Athias Henriot Oudemans (Oudemans Phytoseiidae, Phytoseiidae Phytoseiidae) Saudita (2 C 3 5 UR 2 N seja feminino 937 9 37 9,37 729 7 29 7,29 556 56 5,5 1067 10 67 10,67 838 8 38 8,38 645 6 45 6,4 d 1977 19 77 19,77 1618 16 18 16,18 1394 13 94 13,9 1590 15 90 15,90 1384 84 13,84 1064 64 10,6 2939 39 29,39 2479 24 79 24,79 2064 20 20,6 2542 42 25,42 2194 21 21,94 1739 17 17,3 dias fecundidade 3191 31 91 31,91 3710 37,10 4216 42,1 2175 75 21,75 2684 26 26,84 3056 30,5 fêmea respectivamente 37086 370 86 370,86 38754 387 54 387,54 40583 405 83 405,83 23214 232 14 232,14 26332 263 32 263,32 24885 248 85 248,8 3,02 302 02 (3,02 287 87 2,8 ovos/♀/dia ovosdia dia 2,00 200 00 (2,00 136 1 36 1,3 Ro (Ro 2168 68 21,68 2594 25,94 2952 52 29,5 1895 95 18,95 2025 20,25 22,78 2278 22 78 T (T 2492 92 24,92 2182 82 21,82 1824 18,2 2630 26,30 2360 23 60 23,60 2056 20,5 rm (rm 0181 0 181 0,181 0232 0,232 0248 0,24 0170 170 0,170 0185 185 0,185 0,196 0196 196 λ (λ 1365 365 1,365 1551 551 1,551 1706 706 1,70 1126 126 1,126 1324 324 1,324 1428 428 1,42 temperaturas ( 93 9,3 72 7,2 55 5, 106 8,3 4 6, 197 19,7 161 16,1 139 13, 159 15,9 138 13,8 10, 293 29,3 247 24,7 206 20, 254 25,4 219 21,9 173 17, 319 31,9 371 37,1 421 42, 217 21,7 268 26,8 305 30, 3708 370,8 3875 387,5 4058 40 405,8 2321 232,1 2633 263,3 2488 248, 3,0 (3,0 28 2, 2,0 (2,0 1, 216 21,6 259 25,9 295 29, 189 18,9 202 20,2 22,7 227 249 24,9 218 21,8 182 18, 26,3 236 23,6 205 018 0,18 023 0,23 024 0,2 017 0,17 0,19 019 155 1,55 70 1,7 112 12 1,12 132 1,32 142 1,4 9, 7, 8, 19, 16, 15, 24, 25, 21, 31, 37, 26, 370, 387, 405, 232, 263, 3, (3, (2, 22, 23, 01 0,1 0, 1,5 11 1,1 (3

Abstract The date palm mite, Oligonychus afrasiaticus (McGregor) (Acari: Tetranychidae), is a serious pest of dates in the Middle East and North Africa, inflicting severe economic damage if not controlled early. As predaceous mites are known to be potential biocontrol agents against several pests, so predation capacity, life table, reproduction, and survival of Amblyseius swirskii Athias-Henriot and Neoseiulus cucumeris (Oudemans) (Acari: Phytoseiidae), collected from date palm farms in Qassim Saudi Arabia, were studied under laboratory conditions (25 °C, 30 °C, 35 °C and 50 ± 5% RH) against all motile stages of O. afrasiaticus. For both predators, mean developmental time, oviposition period, and longevity were inversely related to temperature from 25 to 35 °C. Various parameters were studied for A. swirskii and N. cucumeris at 25 °C, 30 °C and 35 °C, i.e. the female developmental time, 9.37, 7.29, 5.56, and 10.67, 8.38, 6.45 d; oviposition period, 19.77, 16.18, 13.94 and 15.90, 13.84, 10.64 d; longevity, 29.39, 24.79, 20.64 and 25.42, 21.94, 17.39 d; fecundity, 31.91, 37.10, 42.16 and 21.75, 26.84, 30.56 eggs per female, respectively. The maximum daily predation rate for both the predators was recorded at 35 °C during the oviposition period. The total predation of A. swirskii and N. cucumeris female was 370.86, 387.54, 405.83, 232.14, 263.32, 248.85 preys at 25 °C, 30 °C and 35 °C respectively. The maximum reproduction rate of A. swirskii and N. cucumeris (3.02, 2.87 eggs/♀/day) was recorded at 35 °C while the minimum (2.00, 1.36 eggs/♀/day) was recorded at 25 °C. The life table parameters were estimated as net reproductive rate (Ro) 21.68, 25.94, 29.52 and 18.95, 20.25, 22.78; the mean generation time (T) 24.92, 21.82, 18.24 and 26.30, 23.60, 20.56 d; the intrinsic rate of increase (rm) 0.181, 0.232, 0.248 and 0.170, 0.185, 0.196; the finite rate of increase (λ) 1.365, 1.551, 1.706 and 1.126, 1.324, 1.428 for A. swirskii and N. cucumeris at 25 °C, 30 °C and 35 °C respectively. The results of this study suggested that the two phytoseiid species are promising biological control agents of O. afrasiaticus at a wide range of temperatures. mite McGregor (McGregor Acari (Acari Tetranychidae, Tetranychidae , Tetranychidae) Africa early pests capacity AthiasHenriot Athias Henriot Oudemans (Oudemans Phytoseiidae, Phytoseiidae Phytoseiidae) Arabia (2 C 3 5 RH O period 2 A N ie i e i.e 937 9 37 9.37 729 7 29 7.29 556 56 5.56 1067 10 67 10.67 838 8 38 8.38 645 6 45 6.4 d 1977 19 77 19.77 1618 16 18 16.18 1394 13 94 13.9 1590 15 90 15.90 1384 84 13.84 1064 64 10.6 2939 39 29.39 2479 24 79 24.79 2064 20 20.6 2542 42 25.42 2194 21 21.94 1739 17 17.3 fecundity 3191 31 91 31.91 3710 37.10 4216 42.1 2175 75 21.75 2684 26 26.84 3056 30.5 respectively 37086 370 86 370.86 38754 387 54 387.54 40583 405 83 405.83 23214 232 14 232.14 26332 263 32 263.32 24885 248 85 248.8 3.02, 302 02 (3.02 287 87 2.8 eggs/♀/day eggsday day 2.00, 200 00 (2.00 136 1 36 1.3 Ro (Ro 2168 68 21.68 2594 25.94 2952 52 29.5 1895 95 18.95 2025 20.25 22.78 2278 22 78 T (T 2492 92 24.92 2182 82 21.82 1824 18.2 2630 26.30 2360 23 60 23.60 2056 20.5 rm (rm 0181 0 181 0.181 0232 0.232 0248 0.24 0170 170 0.170 0185 185 0.185 0.196 0196 196 λ (λ 1365 365 1.365 1551 551 1.551 1706 706 1.70 1126 126 1.126 1324 324 1.324 1428 428 1.42 temperatures ( 93 9.3 72 7.2 55 5.5 106 8.3 4 6. 197 19.7 161 16.1 139 13. 159 15.9 138 13.8 10. 293 29.3 247 24.7 206 20. 254 25.4 219 21.9 173 17. 319 31.9 371 37.1 421 42. 217 21.7 268 26.8 305 30. 3708 370.8 3875 387.5 4058 40 405.8 2321 232.1 2633 263.3 2488 248. 3.02 (3.0 28 2. 2.00 (2.0 1. 216 21.6 259 25.9 295 29. 189 18.9 202 20.2 22.7 227 249 24.9 218 21.8 182 18. 26.3 236 23.6 205 018 0.18 023 0.23 024 0.2 017 0.17 0.19 019 155 1.55 70 1.7 112 12 1.12 132 1.32 142 1.4 9. 7. 5. 8. 19. 16. 15. 24. 25. 21. 31. 37. 26. 370. 387. 405. 232. 263. 3.0 (3. 2.0 (2. 22. 23. 01 0.1 0. 1.5 11 1.1 3. (3

Resumo O psilídeo de concha, Glycaspis brimblecombei Moore, 1964 (Hemiptera: Aphalaridae), um inseto praga originário da Austrália e que se alimenta apenas de plantas de Eucalyptus L'Hér. (Myrtales: Myrtaceae), se espalhou por vários países. Esse inseto, geralmente, atinge grandes populações em plantas de Eucalyptus desde sua entrada no Brasil e, também, indicou um comportamento diferente. Os objetivos deste estudo foram descrever uma adaptação peculiar no hábito alimentar de G. brimblecombei e registrar o novo hábito. A oviposição e alimentação por G. brimblecombei, geralmente, nas folhas de Eucalyptus, passaram a ocorrer, também, em ramos lignificados. Isso sugere uma adaptação diferente desse inseto para reduzir a competição intraespecífica inseto × planta.

Abstract The red gum lerp psyllid, Glycaspis brimblecombei Moore, 1964 (Hemiptera: Aphalaridae), an insect pest originating in Australia and which feeds only on Eucalyptus L'Hér. (Myrtales: Myrtaceae) plants, has spread to several countries. The populations of this insect commonly reach high populations on Eucalyptus plants since its entry into Brazil, and also indicated an unrecorded behavioral. The objectives of this study were to describe a peculiar adaptation in the feeding habit of G. brimblecombei and to register the new habit. The oviposition and feeding by G. brimblecombei, commonly, on the leaves of Eucalyptus, started to occur, also, on lignified twigs. This suggests a not yet recorded adaptation of this insect to reduce insect × plant intraspecific competition.

ABSTRACT: The use of plant resistance acts by intervening in the herbivore-host relationship, through morphological, physical or chemical factors of the plant. This study evaluated the oviposition and foliar consumption of Chrysodeixis includens (Walker [1858]) in soybean genotypes, in free- and no-choice tests, correlating them with the factors, density and size of trichomes. The experiments were carried out in laboratory (25 ± 2 °C; RH= 70 ± 10%; photoperiod 14h) using five cultivars (BRS 391, BRS 6203 RR, BMX Valente RR, Tec Irga 6070 RR, BMX Icone Ipro) and two isolines (PELBR 10-6000 and PELBR 10-6049). The trichomes reported were filiform tectors and claviform multicellular glandular. The density of glandular trichomes, in stages V2 and V5, was higher on BRS 6203 RR and BRS 391, respectively. The higher density of glandular trichomes was observed in V5 and a higher density of tector trichomes in V2. The lowest densities and the smallest sizes of trichomes in V2 and V5 stages was observed on PELBR 10-6049. The size of tector trichomes and the number of eggs did not differ among the cultivars. Foliar consumption was lower for on BMX Icone Ipro and Tec Irga 6070 RR. Trichome density influences the consumption and oviposition behavior of C. includens. ABSTRACT herbivorehost herbivore host relationship morphological Walker 1858 [1858] genotypes free nochoice no choice tests 25 (2 °C C RH 7 10% 10 14h h 391 620 607 106000 6000 10-600 106049. 106049 6049 . 10-6049) V respectively 6049. 10-6049 185 [1858 ( 1 39 62 60 10600 600 10-60 10604 604 10-604 18 [185 3 6 1060 10-6 [18 106 10- [1 [

RESUMO: O uso da resistência de plantas atua intervindo na relação herbívoro-hospedeiro, através de fatores morfológicos, físicos ou químicos da planta. O objetivo deste trabalho foi avaliar a oviposição e o consumo foliar de Chrysodeixis includens (Walker [1858]) em genótipos de soja, através de testes com e sem escolha, correlacionando-os com os fatores, densidade e tamanho de tricomas. Os experimentos foram realizados em laboratório (25 ± 2 °C; UR= 70 ± 10%; fotoperíodo 14h) utilizando cinco cultivares (BRS 391, BRS 6203 RR, BMX Valente RR, Tec Irga 6070 RR, BMX Icone Ipro) e duas linhagens (PELBR 10-6000 e PELBR 10-6049). Os tricomas encontrados foram tectores filiformes e glandulares multicelulares claviformes. A densidade de tricomas glandulares nos estágios V2 e V5, foi maior em BRS 6203 RR e BRS 391, respectivamente. A maior densidade de tricomas glandulares foi observada em V5 e a maior densidade de tricomas tectores em V2. As menores densidades e os menores tamanhos de tricomas nos estágios V2 e V5 foram observados em PELBR 10-6049. O tamanho de tricomas tectores e o número de ovos não diferiram entre as cultivares. O consumo foliar foi menor para BMX Icone Ipro e Tec Irga 6070 RR. A densidade de tricomas influência o comportamento de consumo e oviposição de C. includens. RESUMO herbívorohospedeiro, herbívorohospedeiro herbívoro hospedeiro, hospedeiro herbívoro-hospedeiro morfológicos planta Walker 1858 [1858] soja escolha correlacionandoos correlacionando 25 (2 °C C UR 7 10% 10 14h h 391 620 607 106000 6000 10-600 106049. 106049 6049 . 10-6049) claviformes V respectivamente 6049. 10-6049 185 [1858 ( 1 39 62 60 10600 600 10-60 10604 604 10-604 18 [185 3 6 1060 10-6 [18 106 10- [1 [

ABSTRACT Duponchelia fovealis is an important pest in strawberry crops. In search of an alternative biological control method, the objective of this study was to assess the effects of a diet composed of Ephestia kuehniella or D. fovealis eggs offered to Chrysoperla externa larvae on their subsequent development and survival under controlled conditions. Biological and reproductive parameters of C. externa were assessed. Additionally, the daily predation of D. fovealis eggs by C. externa was analyzed. Finally, a fertility life table was constructed. The egg-to-pupa development time differed significantly between diets. The weights of firstand second-generation male C. externa pupae were significantly higher when fed on D. fovealis eggs. Males tended to live longer on a diet based on D. fovealis eggs, but females presented no significant differences between diets. The oviposition period tended to be longer for C. externa fed on D. fovealis eggs. The time between generations and the net reproductive rate were greater for C. externa fed on D. fovealis eggs. The results showed that D. fovealis eggs are a suitable diet for the development of C. externa. This information is important for developing protocols for the use of C. externa as a biocontrol agent against this pest. crops method D conditions C assessed Additionally analyzed Finally constructed eggtopupa egg pupa diets secondgeneration second generation

RESUMO Duponchelia fovealis é uma praga severa no cultivo do morangueiro. Na busca de uma alternativa de agente de controle biológico, avaliamos o impacto de uma dieta composta por ovos de Ephestia kuehniella ou D. fovealis oferecidas às larvas de Chrysoperla externa em seu subsequente desenvolvimento e sobrevivência, sob condições controladas. Foram avaliados os parâmetros biológicos e reprodutivos de C. externa e a predação diária de ovos de D. fovealis por C. externa. Por fim, foi construída a tabela de vida de fertilidade. A duração do período do ovo à pupa diferiu significativamente entre as dietas. O peso das pupas de C. externa foi significativamente maior quando alimentadas com ovos de D. fovealis para machos de primeira e segunda geração. Os machos tenderam a viver mais tempo com uma dieta baseada em ovos de D. fovealis, mas não foram detectadas diferenças significativas entre as dietas para as fêmeas. O período de oviposição tendeu a ser mais longo em C. externa alimentada com ovos de D. fovealis. A predação média diária da primeira geração de C. externa foi afetada pela dieta. O tempo entre cada geração e a taxa líquida de reprodução foram maiores para C. externa alimentada com ovos de D. fovealis do que quando alimentada com ovos de E. kuehniella. Nossos resultados comprovaram que os ovos de D. fovealis são uma dieta adequada para o desenvolvimento de C. externa. Esta informação é valiosa no desenvolvimento de protocolos para o uso de C. externa como agente de controle desta praga. morangueiro biológico D sobrevivência controladas C fim fertilidade fêmeas E

ABSTRACT Resistant genotypes are a valuable tool in integrated pest management programs of insect pests of stored grains. Here, we screened 90 Brazilian common bean genotypes to select promising sources of resistance to Acanthoscelides obtectus. Thus, bean samples were infested with newly emerged insects, and the number of eggs, emerged insects, developmental time, and larval viability were assessed in a no-choice test. Genotypes IPR Garça, CHIR 13, IAC Harmonia, CHIR 20, IAC Boreal, and CHIP 312 showed low oviposition. Genotypes IPR Quero-Quero, BRS Notável, CHIC 61 - Cariocão, BRS Ametista, IPR Uirapuru, IAC Imperador, Avaluna, CHP 04-241A-212 - Guapo B. × Uirapuru, CHIB 06, SCS 206 - Potência, CHC 01-175-1 - Campeiro × IAC Tibatã, CHP 01-182-48 - Uirapuru × Campeiro, UEM 266, CHC 04-233-2 - Siriri × Horizonte, CHV 17-64, SCS 205 - Riqueza, CHP 01-238-80 - MN 13337, SM 1510 - preto, BRS Radiante, CHIB 18, CHIR 14, CAV 17 EFM 008, CHIR 04, IPR Campos Gerais, CHV 05-268-02, LP 13-624 - preto, Linhagem 110 - IAC, CHP 01-182-12 - Uirapuru × Campeiro, LP 13-84 - carioca, BRS Esteio, CHV 17-635, CHB 15-518, Arcelina 1, IPR Tuiuiu, SCS 204 - Predileto, TB - 17-02, BRS Estilo, SM 0511 - cavalo preto, Pérola, IPR Tangará, and CNFRj - 15411 reduced oviposition, the number of emerged adults, and larval viability, suggesting antixenosis and/or antibiosis. Thus, these genotypes are promising sources of resistance factors and can be used in breeding programs to obtain lines of common beans resistant to A. obtectus. grains Here 9 obtectus Thus insects eggs time nochoice no choice test Garça 13 Harmonia 20 Boreal 31 oviposition QueroQuero, QueroQuero Quero Quero, Quero-Quero Notável 6 Cariocão Ametista Imperador Avaluna 04241A212 A 04 241A 212 04-241A-21 B 06 Potência 011751 01 175 1 01-175- Tibatã 0118248 182 48 01-182-4 266 042332 233 2 04-233- Horizonte 1764, 1764 64, 64 17-64 Riqueza 0123880 238 80 01-238-8 13337 151 preto Radiante 18 14 008 Gerais 0526802, 0526802 05 268 02, 02 05-268-02 13624 624 13-62 11 0118212 12 01-182-1 1384 84 13-8 carioca Esteio 17635, 17635 635, 635 17-635 15518, 15518 15 518, 518 15-518 Tuiuiu Predileto 1702, 1702 17-02 Estilo 051 Pérola Tangará 1541 adults andor or antibiosis 3 04241A21 0 21 04-241A-2 01175 01-175 011824 4 01-182- 26 04233 23 04-233 176 17-6 012388 8 01-238- 1333 00 052680 05-268-0 1362 62 13-6 011821 138 13- 1763 63 17-63 1551 51 15-51 170 17-0 154 04241A2 04-241A- 0117 01-17 01182 01-182 0423 04-23 17- 01238 01-238 133 05268 05-268- 136 155 5 15-5 04241A 04-241A 011 01-1 0118 01-18 042 04-2 0123 01-23 0526 05-268 15- 01- 04- 012 01-2 052 05-26 05-2 05-

Abstract The technique of terrestrial sampling of boll weevil (BW) populations is expensive and inefficient over large areas, but may be cheaper and more efficient without involving the manipulation of cotton squares. The aim of this study was to develop a technique to sampling cotton squares based on the observation of opened and/or yellowing bracts to determine the need and efficacy of chemical control of BW in cotton crops. The first experiment aimed to estimate the ratio between the number of cotton squares with opened and/or yellowed bracts and that of squares with BW oviposition punctures. The second experiment, aimed to determine the efficacy of chemical control for BW by sampling cotton squares with opened and/or yellowed bracts. The ratio between the number of opened and/or yellowed bracts and the number of cotton squares with oviposition punctures was 2:1. The level and efficiency of chemical control of BW, based on the percentage and sampling of cotton plants with opened and/or yellowed bracts, was 5% and did not differ from the one based on the observation of cotton plants with 10% cotton squares with oviposition punctures by BW females. The control level based on sampling cotton plants with open and/or yellowing bracts was 5%. The efficiency of chemical insecticides using this economic threshold against the BW did not differ from that based on sampling cotton plants with 10% of cotton squares with oviposition punctures by BW females. This indicates that the chemical control of cotton boll weevil can be carried out based on cotton squares with open and/or yellowed bracts. (BW areas andor or crops 21. 21 2 1. 1 2:1 5 10 females 2:

Resumo A técnica de amostragem terrestre de populações do bicudo-do-algodoeiro é cara e ineficiente em grandes áreas, mas pode ser mais barata e mais eficiente sem envolver a manipulação dos botões florais de algodoeiros. O objetivo deste estudo foi desenvolver uma técnica de amostragem de botões florais de algodoeiros, baseada na observação de brácteas abertas e/ou amarelecidas, para determinar a necessidade de controle químico do bicudo em lavouras de algodão. O primeiro experimento teve por objetivo estimar a razão entre o número de botões florais de algodoeiros com brácteas abertas e/ou amarelecidas e o daqueles com orifícios de oviposição. O segundo experimento teve por objetivo determinar a eficiência do controle químico do bicudo por meio da amostragem de botões florais de algodoeiros com brácteas abertas e/ou amarelecidas. A razão entre o número de brácteas abertas e/ou amarelecidas e o de botões florais de algodoeiros com orifícios de oviposição foi de 2:1. O nível de controle, baseado na amostragem de algodoeiro com brácteas abertas e/ou amarelecidas, foi de 5%. A eficiência do inseticida químico utilizando esse limiar econômico contra o bicudo não diferiu daquela baseada na amostragem de plantas de algodão com 10% de botões florais com orifícios de oviposição por fêmeas desse inseto. O controle químico do bicudo-do-algodoeiro pode ser realizado a partir de amostragens de brácteas abertas e/ou amarelecias em plantas de algodoeiro. bicudodoalgodoeiro áreas eou ou 21. 21 2 1. 1 2:1 5 5% 10 inseto 2:

ABSTRACT Zygothrica is a genus of Drosophilidae (Diptera) whose species utilize flowers and fungi for breeding sites, with records of fungi being used as courtship arena. Due to this habit, its representation in Drosophilidae surveys using banana-baited traps is generally low. However, Zygothrica orbitalis was well represented in a few samples with these traps. In this study, we report for the first time the breeding site of Z. orbitalis in living fruits of Psychotria brachyceras (Rubiaceae), noting that the use of living fruits is rare among Drosophilidae. The fructification of the plant occurs in the area of study from May to August, with previous collection records of the species in the Restinga (sandbank or strand) forest. Additionally, the emergence of some individuals of the invasive species Drosophila suzukii was observed, which highlights the necessity for continuous study of this plant to understand the dynamics between a native and an exotic species. Besides the ecological importance, our results are relevant for understanding the evolution of trophic resource use by the Zygothrica genus. Diptera (Diptera sites arena habit bananabaited banana baited low However Z Rubiaceae, Rubiaceae , (Rubiaceae) August sandbank strand forest Additionally observed importance (Rubiaceae

ABSTRACT Synthetic chemical insecticides are widely used in population control of pests. Aedes aegypti is the primary vector of dengue, Zika, chikungunya and yellow fever to humans, and has proven resistance to chemical insecticides. As an alternative vector control method, the ethanolic extract of the leaves of Piper peltatum L. (Piperaceae) showed larvicidal activity against Ae. aegypti. Despite the wide medicinal use of this plant, the biological activity of its isolated constituents remains unexplored. In this sense, we isolated, identified and evaluated the larvicidal activity of 4-nerolidylcatechol (4-NC) from P. peltatum against Ae. aegypti, Culex quinquefasciatus and Anopheles darlingi, focusing on the larvicidal, adulticidal and genotoxic potential of 4-NC on Ae. aegypti. Larvae were captured in the city of Manaus, Amazonas state, Brazil. 4-NC was isolated from the extract of the leaves of P. peltatum via silica gel chromatography. This was identified using nuclear magnetic resonance spectroscopy and tested in Artemia franciscana (6.25, 12.5, 25, 50, 100 and 200 µg/mL). In the toxicity bioassay, Ae. aegypti larvae were exposed to 30, 50, 70, 90, and 110 µg/mL of 4-NC, while Cx. quinquefasciatus and An. darlingi were exposed to 6.25, 12.5, 25, 50 and 100 µg/mL. Ae. aegypti larvae were also subjected to 40 and 60 µg/mL of 4-NC (genotoxicity bioassay), and adult females to 62.5 to 1,000 µg/mL (adulticidal bioassay). The results of the 4-NC toxicity assays showed that there was 100% mortality in larvae of Ar. franciscana at the concentration of 200 µg/mL, with an LC50 of 8.0 μg/mL. In the larvae of Ae. aegypti, mortality was 100%, with an LC50 of 62 μg/mL. In larvae of Cx. quinquefasciatus, 97% mortality occurred, with an LC50 of 52.3 μg/mL, and in An. darlingi larvae there was an 83% mortality rate, with an LC50 of 55.8 μg/mL. In adults of Ae. aegypti, however, there was no adulticidal activity. In the larvae of Ae. aegypti, the genotoxic effect of 4-NC (40 and 60 µg/mL) showed significant frequency (p < 0.05) of cellular abnormalities (micronuclei, budding and nuclear bridges) of interphasic nuclei of neuroblasts and oocytes in relation to the negative control. This result may be associated with a decrease in oviposition of females, which was observed in two generations. We can confirm that 4-NC has larvicidal activity against Ae. aegypti, Cx. quinquefasciatus and An. darlingi. Although it does not present adulticidal activity in Ae. aegypti, it reduced the oviposition of females. Therefore, 4-NC seems to be a strong candidate for the development of an alternative method for the control of these mosquitoes in the immature phase. pests dengue Zika humans L Piperaceae (Piperaceae Ae plant unexplored sense 4nerolidylcatechol nerolidylcatechol 4 4NC NC (4-NC P Manaus state Brazil chromatography 625 6 25 (6.25 125 12 5 12.5 10 20 µgmL . µg mL bioassay 30 70 90 11 4NC, NC, Cx An 6.25 genotoxicity , bioassay) 62. 1000 1 000 1,00 bioassay. Ar LC LC5 80 8 0 8. μgmL μg μg/mL 97 occurred 523 52 3 52. 83 rate 558 55 55. however (4 p 0.05 005 05 micronuclei, micronuclei (micronuclei bridges generations Therefore phase 2 (6.2 12. 7 9 6.2 00 1,0 ( 0.0 (6. 6. 1, 0. (6