Acetic acid bacteria (AAB) are versatile organisms that catalyze the conversion of a wide range of carbon sources into biomolecules of great industrial interest. In this study, we exploited the ability of Gluconobacter spp. to synthesize levans. Among the isolated strains, Gluconobacter cerinus UELBM11 produced approximately 14.0 g L−1 of levan under non-optimized conditions. Gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) analyses confirmed that levan obtained from G. cerinus UELBM11 consisted of a β-(2→6)-D-fructose backbone with some β-(2→1) ramifications. The average molecular weight (Mw) of the purified levan was 8.78 × 105 Da. Thermogravimetric/differential thermogravimetric (TGA/DTG) analysis indicated high thermal stability, with the maximum decomposition rate observed at 227.44 °C. Scanning electron microscopy (SEM) revealed a microporous morphology, and the antioxidant activity assays demonstrated that levan had a high scavenging capacity of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl radicals. Therefore, it has been demonstrated the levan produced by G. cerinus UELBM11 is a promising natural antioxidant and, owing to its microporosity and excellent thermal properties and stability, is a potential candidate as an additive in cosmetics, pharmaceuticals, and food products. AAB (AAB interest study spp levans strains UELBM UELBM1 140 14 0 14. L1 L 1 L− nonoptimized non optimized conditions chromatographymass chromatography mass GCMS, GCMS GC MS , (GC-MS) FTIR, FTIR (FTIR) NMR (NMR G β2→6Dfructose β26Dfructose βDfructose β 2→6 D fructose 2 6 β2→1 β21 2→1 β-(2→1 ramifications Mw (Mw 878 8 78 8.7 10 Da Thermogravimetricdifferential Thermogravimetric differential TGA/DTG TGADTG TGA DTG (TGA/DTG stability 22744 227 44 227.4 C °C SEM (SEM morphology 2,2azinobis 22azinobis azinobis 2,2 azino bis 3ethylbenzothiazoline6sulfonic ethylbenzothiazolinesulfonic 3 ethylbenzothiazoline sulfonic ABTS (ABTS radicals Therefore cosmetics pharmaceuticals products (GC-MS (FTIR β2 6Dfructose Dfructose 26 2→ β2→ 21 β-(2→ 87 7 8. 2274 22 4 227. 2azinobis 2, β-(2 β-( β-

Computational predictive approaches present themselves as an attractive alternative in the search for new compounds that may present toxic or growth regulatory activity in Aedes aegypti. Aspergillus species is a producer of secondary metabolites with diverse biological activities. In this study, it was possible to evaluate the pharmacokinetic and toxicological properties in silico, as well as the prediction of the insecticidal biological activities of the juvenile hormone of natural substances produced by the genus Aspergillus. Initially, 187 molecules were cataloged from various species of Aspergillus spp., and only seven molecules of indole alkaloids were found to exhibit a toxic dose 50% (TD50) after conducting toxicity predictions. The molecular dynamic simulation trajectories were utilized to study parameters such as root mean square deviation, root mean square fluctuation, radius of gyration and intermolecular hydrogen bond to understand the behavior and stability of proposed compounds in the binding pocket of the target protein. The N-β-acetyltryptamine molecule showed a binding affinity value of –8.100 ± 0.200 kcal mol-1, which classifies the compound as a potential insecticidal agent against the Aedes aegypti vector. silico Initially 18 spp spp. 50 TD50 TD (TD50 predictions deviation fluctuation protein Nβacetyltryptamine N β acetyltryptamine 8100 8 100 –8.10 0200 0 200 0.20 mol1, mol1 mol 1, 1 mol-1 vector 5 TD5 (TD5 810 10 –8.1 020 20 0.2 mol- (TD 81 –8. 02 2 0. –8 –

The history of the discovery, use, and development of dyes is closely linked to the history of humanity itself. Among the first records of human civilization are cave paintings made from, e.g., fruits, plants, minerals, and insects. Over the centuries, using different techniques, colors have been given to all types of materials and products, from clothing to food. The chemicals responsible for colors are dyes - a vast collection of molecules of the most varied compositions, but which have in common the absorption of light within the visible window of the electromagnetic spectrum. This article aims not only to review the properties of some classes of natural dyes, their origins, and their applications but also to describe the various computational chemistry methods involved in designing new bio-inspired molecules. discovery use itself eg e g e.g. fruits plants minerals insects centuries techniques products food compositions spectrum origins bioinspired bio inspired e.g

RESUMO A produção de grãos apresenta crescimento constante, entretanto, a capacidade estatística de armazenamento permanece deficitária no Brasil. A falta de estruturas e a precariedade e inadequação no armazenamento ocasionam perdas na pós-colheita, devido ação de fatores que interagem com os grãos, culminando na sua deterioração. Para reversão desses efeitos, o tratamento químico é realizado, contudo, busca-se por sua substituição por substâncias naturais devido a implicações ambientais e medicinais. Objetivou-se com esta revisão, a discussão de uma potencial estratégia sustentável para o tratamento de grãos armazenados, com foco no uso de compostos naturais com propriedades bioativas como antifúngica, antioxidante e inseticida, que os torna potenciais para serem estudados. Foram delineados compostos naturais que possuem propriedades úteis para aplicação no armazenamento de grãos. Pesquisas a respeito do emprego dos compostos naturais no tratamento de grãos são incipientes, carecendo de avanços na identificação de novos compostos promissores, melhor compreensão dos mecanismos de ação e otimização/desenvolvimento de processos de extração, aplicação e formulação eficazes que viabilizem o futuro uso das substâncias. constante entretanto Brasil póscolheita, póscolheita pós colheita, colheita pós-colheita deterioração efeitos realizado contudo buscase busca se medicinais Objetivouse Objetivou revisão armazenados antifúngica inseticida estudados incipientes promissores otimizaçãodesenvolvimento otimização desenvolvimento extração

ABSTRACT Grain production is steadily increasing; however, the static storage capacity remains inadequate in Brazil. The lack of proper facilities and the precarious and inadequate storage conditions lead to post-harvest losses due to factors interacting with the grains, causing their deterioration. Chemical treatments are commonly used to reverse these effects; however, their replacement with natural substances has been sought due to environmental and human health concerns. The objective of this review was to discuss a sustainable strategy for treating stored grains, focusing on the use of natural compounds with bioactive properties, such as antifungal, antioxidant, and insecticidal activities, with potential for further studies. Natural compounds with useful properties for application in grain storage were outlined. Research on the use of natural compounds in grain treatment is still in its early stages, requiring progress in identifying new promising compounds, understanding the mechanisms of action, and developing and optimizing effective extraction, application, and formulation processes to make the use of these substances viable. increasing however Brazil postharvest post harvest grains deterioration effects concerns antifungal antioxidant activities studies outlined stages action extraction viable

In silico and in vitro tests can reveal promising anti-leishmania activity for natural products and their derivatives. The aim of this study was to investigate in silico the pharmacological activities of potential new chalcones and their leishmanicidal potential in vitro. The in silico study was carried out using the PASS, MolPredictX and Molegro Virtual Docker 6.0 programs. Antiparasitic activity was assessed in axenic promastigote and amastigote forms of Leishmania braziliensis. The cytotoxicity tests used the J77G8 cell line. The chalcones exhibited 50% cytotoxic concentration values (CC50) values > 50 μM. Chalcone 4 (named FERAI) presented the best activity with concentration for 50% of promastigotes and intracellular parasites forms (EC50) of 9.75 ± 1.7 and 10.13 ± 1.7 μM for promastigote and amastigote, respectively. Reactive oxygen species (ROS) testing presented increased ROS levels in the parasite at the FERAI concentrations of 10 μM (56.33%), 20 μM (61.76%) and 30 μM (67.13%). Molecular docking revealed interactions (binding energy) between FERAI and the enzymes UDP-glycosyl pyrophosphorylase (-56.8384), dihydroorotate-dehydrogenase (-132.276) and trypanothione-reductase (-151.281). Our results demonstrated the anti-leishmanial activity of chalcones, especially FERAI, with a noted raising of ROS levels in the parasite. Molecular docking revealed dihydroorotate dehydrogenase and trypanothione reductase as potential pharmacological targets for FERAI. antileishmania anti leishmania derivatives PASS 60 6 0 6. programs braziliensis JG J G J77G line CC50 CC (CC50 5 named EC50 EC (EC50 975 9 75 9.7 17 1 7 1. 1013 13 10.1 respectively (ROS 56.33%, 5633 56.33% , 56 33 (56.33%) 2 61.76% 6176 61 76 (61.76% 3 67.13%. 6713 67.13% . 67 (67.13%) binding energy UDPglycosyl UDP glycosyl 56.8384, 568384 56.8384 8384 (-56.8384) dihydroorotatedehydrogenase 132.276 132276 132 276 (-132.276 trypanothionereductase 151.281. 151281 151.281 151 281 (-151.281) antileishmanial leishmanial CC5 (CC5 EC5 (EC5 97 9. 101 10. 563 56.33 (56.33% 61.76 617 (61.76 671 67.13 (67.13% 56838 56.838 838 (-56.8384 132.27 13227 27 (-132.27 15128 151.28 15 28 (-151.281 (CC (EC 56.3 (56.33 61.7 (61.7 67.1 (67.13 5683 56.83 83 (-56.838 132.2 1322 (-132.2 1512 151.2 (-151.28 56. (56.3 61. (61. 67. (67.1 568 56.8 8 (-56.83 132. (-132. 151. (-151.2 (56. (61 (67. (-56.8 (-132 (-151. (56 (6 (67 (-56. (-13 (-151 (5 ( (-56 (-1 (-15 (-5 (-

Lantana caatingensis Moldenke (Verbenaceae) is an endemic plant from northeastern Brazil. However, its chemical composition is almost unknown. This study aimed to characterize the chemical compounds found in L. caatingensis and investigate the antileishmanial potential of its hydroalcoholic extract and polar fractions. Compounds present in the hydroalcoholic extract obtained from L. caatingensis leaves and its polar fractions (ethyl acetate and methanolic) were analyzed by liquid chromatography with a photodiode-array detector coupled to electrospray ionization quadrupole time-of-flight mass spectrometry. An antileishmanial assay was performed in a 96-well plate against Leishmania major promastigotes using resazurin on a plate spectrophotometer and the results were expressed as percentage of growth inhibition. Nine compounds were determined in the investigated extract and fractions, classified as flavonoids, flavonoid C-glycosides and phenylethanoid glycosides. The antileishmanial activity revealed that the hydroalcoholic extract and the ethyl acetate fraction were active against L. major promastigotes with inhibitory concentration of 71.78 and 26.86 µg mL-1, respectively, while the methanolic fraction was inactive (478.40 µg mL-1). This is the first report on the chemical composition and antileishmanial activity of L. caatingensis extracts. These results contribute to further knowledge of species belonging to the Lantana genus. Verbenaceae (Verbenaceae Brazil However unknown L photodiodearray photodiode array timeofflight time flight spectrometry 96well well 96 inhibition flavonoids Cglycosides C glycosides 7178 71 78 71.7 2686 26 86 26.8 mL1, mL1 mL 1, 1 mL-1 respectively 478.40 47840 478 40 (478.4 mL1. . mL-1) extracts genus 9 717 7 71. 268 2 8 26. mL- 478.4 4784 47 4 (478. 478. (478 (47 (4 (

ABSTRACT The studies carried out demonstrate the potential and shortcomings in the development of capabilities in this sense. The work is part of the project Professional training for educational and sociocultural actors and agencies in regional and local historical studies for sustainable development in the central region of Cuba. The actions are carried out from an innovative, interdisciplinary and professional approach that allows the analysis of the natural, social and historical reality in relation to good practices of employment, conservation and protection of heritage resources for the enhancement. Links are produced between the results of regional and local historical studies with local development, based on the interrelation between university and society. It includes the development of social innovation products resulting from the theoretical, methodological, didactic and practical contributions on regional and local historical studies produced by the Department of History of the University of Cienfuegos that favor management in the sustainable development of localities and communities of the central region of Cuba.

RESUMEN Los estudios realizados demuestran las potencialidades y carencias en el desarrollo de capacidades en este sentido. El trabajo forma parte del proyecto Formación profesional a los actores y agencias educativas y socioculturales en estudios históricos regionales y locales para el desarrollo sostenible en la región central de Cuba. Las acciones se realizan desde un enfoque innovador, interdisciplinario y profesionalizadoque permite el análisis de la realidad natural, social e históricaen relación a las buenas prácticas de empleo, conservación y protección de los recursos patrimonialespara la puesta en valor. Se producen articulaciones entre los resultados de los estudios históricos regionales y locales con el desarrollo local, a partir de la interrelación entre universidad y sociedad. Se incluye la elaboración de productos de innovación social resultantes de los aportes teóricos, metodológicos, didácticos y prácticos sobre los estudios históricos regionales y locales producidos por el departamento de Historia de la Universidad de Cienfuegos que favorecen la gestión en el desarrollo sostenible de las localidades y comunidades de la región central de Cuba.

Abstract Considering that currently in Mexico there is a trend toward greater consumption of natural products that benefit health and that, in the long term, do not produce adverse effects, the objective of this research is to evaluate if the main extracts and by-products derived from the roselle flower can be considered strategic, based on its properties, impact on health, value chain and that Mexico is one of the 10 main producers worldwide. For this, the following method was used: a) research on the roselle flower and its benefits to humans were compiled and analyzed; b) the information was analyzed with the Delphi tool; c) a dendrogram was developed to categorize the relevance in its use, and, d) the value chain of the processes that make up its supply chain was analyzed to determine its conditions and potential. The results show that it does not present toxic or mutagenic activity and that it has a great impact in 3 aspects: alternative medicine, as a disinfectant and food supplement. It was found that its value chain is incipient, but with potential in: planting regions, research centers and links with the industrial sector for its use, marketing and sales coverage.

Resumen Actualmente, en México existe la tendencia hacia un mayor consumo de productos naturales que beneficien la salud y a largo plazo no produzcan efectos negativos, por lo cual, el objetivo de esta investigación es evaluar si los principales extractos y subproductos de la flor de jamaica se pueden considerar estratégicos, en función de sus propiedades, impacto en la salud, cadena de valor, pues México es uno de los 10 principales productores a nivel mundial. Para esto, se utilizó el siguiente método: a) se recopilaron y analizaron investigaciones sobre la flor de jamaica y beneficios al ser humano; b) se analizó la información con la herramienta Delphi; c) se desarrolló un dendrograma para categorizar la relevancia en su uso, y, d) se determinaron las condiciones y potencial de la cadena de valor de los procesos que integran su cadena de suministro. Los resultados muestran que no presenta actividad tóxica o mutagénica y que tiene un gran impacto en 3 vertientes: medicina alternativa, como desinfectante y complemento alimenticio. Se encontró que su cadena de valor es incipiente, pero con potencial en: regiones de siembra, centros de investigación y vinculación con el sector industrial para su aprovechamiento, cobertura de comercialización y venta.

Resumo O noroeste pacífico da Costa Rica é uma região com uma estacionalidade no regime de chuvas muito marcada. Somado a essa sazonalidade, esta área da Costa Rica é propensa a fenômenos hidroclimáticos extremos como secas e inundações, por exemplo. A distribuição limitada de pluviômetros ou a falta de disponibilidade de informações relevantes fazem com que os dados complementares obtidos de satélites e suas respectivas reanálises sejam fundamentais para obter informações cruciais e, assim, embasar as ações de gestão dos recursos hídricos e suas repercussões nos ecossistemas, tanto naturais quanto produtivos. Para analisar os padrões de precipitação, foram analisadas as séries temporais de precipitação do produto CHIRPS para cinco ecorregiões delimitadas na Área de Conservação Guanacaste, no Pacífico noroeste da Costa Rica. Estas curvas foram correlacionadas com as séries temporais das regiões de monitoramento da temperatura superficial do mar nas regiões Niño 1.2, Niño 3, Niño 3.4 e Niño 4. Todas as ecorregiões analisadas correlacionaram de forma negativa e forte com a região do Niño 1.2, com valores de R entre -0.72 e -0.74. Além disso, foi detectado um atraso na curva do Niño 4 de quatro a cinco meses, em relação à curva da região Niño 1.2. O estudo sugere que a anomalia do El Niño 4, com um desfasamento de aproximadamente 4 a 5 meses, pode ser utilizada como indicador de possíveis impactos nos padrões de precipitação nas diferentes ecorregiões estudadas. Este tempo é suficiente para planejar ações, especialmente no setor agroprodutivo. Neste trabalho, é demonstrado o potencial de previsibilidade dos efeitos do fenômeno ENOS sobre o padrão de precipitação em áreas extensas e com certa homogeneidade ecossistêmica, como as ecorregiões na Área de Conservação Guanacaste.

Abstract The Pacific Northwest of Costa Rica is a region with marked seasonality in rainfall patterns. This area of Costa Rica is prone to extreme hydroclimatic phenomena such as droughts and floods. Due to the limited distribution of rainfall gauges and the unavailability of relevant information, complementary data obtained from satellites and their respective reanalyzes become imperative for acquiring crucial information. This information can support water resource management actions and their impacts on both natural and productive ecosystems. To analyze the precipitation patterns, we utilized the CHIRPS product’s precipitation time series for five ecoregions within the Guanacaste Conservation Area, located in the northwestern Pacific region of Costa Rica. These curves were strongly and negatively correlated with time series from sea surface temperature monitoring regions, including Niño 1.2, Niño 3, Niño 3.4, and Niño 4. All analyzed ecoregions exhibited strong negative correlations with the Niño 1.2 region, with correlation coefficients (R values) ranging between -0.72 to -0.74. Additionally, a lag of four to five months was observed in the Niño 4 curve compared to the Niño 1.2 region. This study suggests that the Niño 4 anomaly, with a lag of approximately 4 to 5 months, can serve as an indicator of possible impacts on precipitation patterns in different ecoregions. This provides sufficient time to plan actions, particularly within the agricultural sector. This study demonstrates the potential predictability of the effects of ENSO phenomenon on precipitation patterns for large areas with a certain eco-systemic homogeneity, such as the ecoregions in the Guanacaste Conservation Area.

Resumen El pacífico noroeste de Costa Rica es una región con una estacionalidad en el régimen de lluvias muy marcada. Sumado a dicha estacionalidad, esta área de Costa Rica es propensa a fenómenos hidroclimáticos extremos como sequías e inundaciones, por ejemplo. La escasa distribución de pluviómetros o bien, la no disponibilidad de información pertinente, hacen que los datos complementarios obtenidos de satélites y sus respectivas reanálisis, sean fundamentales para disponer de información crucial y así poder fundamentar las acciones de gestión del recurso hídrico y sus repercusiones en los ecosistemas, tanto naturales como productivos. Para analizar los patrones de precipitación, se analizaron las series de tiempo de precipitación del producto CHIRPS para cinco ecorregiones delimitadas en el Área de Conservación Guanacaste, en el Pacífico noroeste de Costa Rica. Estas curvas se correlacionaron con las series de tiempo de las regiones de monitoreo de la temperatura superficial del mar en las regiones Niño 1.2, Niño 3, Niño 3.4 y Niño 4. Todas las ecorregiones analizadas correlacionaron de forma negativa y fuertemente con la región del Niño 1.2, con valores de R entre -0.72 y -0.74. Por otra parte, se detectó un rezago en la curva de Niño 4 de cuatro a cinco meses, con la curva de la región Niño 1.2. El estudio sugiere que la anomalía del Niño 4, con un desfase de aproximadamente 4 a 5 meses, puede ser utilizada como indicador de posibles impactos en los patrones de precipitación en las diferentes ecorregiones estudiadas. Este tiempo es suficiente para planear acciones, sobre todo en el sector agroproductivo. En este trabajo se muestra el potencial de predictibilidad de los efectos del fenómeno ENOS sobre el patrón de la precipitación para áreas extensas y con cierta homogeneidad ecosistémica, como son las ecorregiones en el Área de Conservación Guanacaste.

RESUMEN La deposición de asfaltenos es un problema que se presenta en las operaciones petroleras afectando a la mayoría de los petróleos, usándose productos inhibidores/dispersantes para su solución. Se realizó una revisión sistemática de literatura científica para analizar productos alternativos evaluados entre 2014-2023, y las características de las investigaciones publicadas. Se utilizó Google Académico y criterios de inclusión y exclusión según la declaración PRISMA, ubicándose en total 24 artículos. Venezuela destaca con la mayor producción de investigaciones, los productos sintéticos fueron más utilizados (líquidos iónicos, resinas y nanopartículas metálicas) y entre los naturales los aceites vegetales. Los métodos de ensayo principales fueron microscopía óptica y absorbancia, los análisis se basaron principalmente en descripción/comparación y se visualizaron 4 aceites vegetales y 5 productos sintéticos con eficiencias mayores a 75 %. Se resalta la necesidad de investigación con productos naturales por ser de más fácil obtención, menos costosos y amigables con el ambiente.

ABSTRACT Asphaltene deposition is a problem that occurs in oil operations, affecting most types of crude oil. Inhibitor/dispersant products are used to address this issue. A systematic review of scientific literature was conducted to analyze alternative products evaluated between 2014 and 2023, as well as the characteristics of the published research. Google Scholar was used along with inclusion and exclusion criteria according to the PRISMA statement, resulting in a total of 24 articles. Venezuela stands out with the highest production of research. Synthetic products were more commonly used (ionic liquids, resins, and metallic nanoparticles), and among natural products, vegetable oils were prominent. The main testing methods were optical microscopy and absorbance. The analyses were primarily based on description/comparison, and four vegetable oils and five synthetic products were found to have efficiencies greater than 75%. The need for research with natural products is highlighted, as they are more easily obtainable, less expensive, and environmentally friendly.

Abstract Due to the irrational use of chemicals for pest control in intensive crops, the diversity of communities of beneficial organisms, such as the predatory mites of the order Mesostigmata, has been alarmingly diminished,. These arachnids have taken on agricultural relevance because most of their members have zoophagic feeding habits and naturally regulate harmful populations of insects, nematodes and other mites that inhabit plants. For more than 40 years, predatory mites have been introduced regularly for biocontrol work and it is estimated that more than 60% of programs use these organisms; however, the commercial products available are limited to a few species, which vary in effectiveness according to the conditions of the crop, availability of prey and competition for niches with other predatory organisms; in addition, their production is protected under trade secrecy in most cases. With this, the opportunity opens up to explore native or even already commercialized species in order to evaluate their predatory capacity at the research level or as usual tools for pest regulation; a first challenge for this purpose is propagation and culture methods. The methods for mite culture can be divided into two: maintenance on plants and in confinement; although each technique has been developed for a particular species or a certain purpose, knowing their basic design promises to bring us closer to a new line of study for their introduction and reintroduction into agricultural systems.

Resumen Debido al uso irracional de productos químicos para el control de plagas en los cultivos intensivos, se han disminuido de manera alarmante la diversidad de comunidades de organismos benéficos, tal es el caso de los ácaros depredadores del orden Mesostigmata. Estos arácnidos han tomado relevancia agrícola debido a que, la mayoría de sus miembros presentan hábitos alimentarios zoofágicos y regulan de manera natural poblaciones perjudiciales de insectos, nematodos y otros ácaros que habitan las plantas. De manera regular, desde hace más de 40 años se introducen los ácaros depredadores para los trabajos de biocontrol y se estima que más de 60% de los programas utilizan estos organismos; sin embargo, los productos comerciales disponibles están limitados a unas pocas especies, las cuales varían en efectividad según las condiciones del cultivo, disponibilidad de presas y competencia de nichos con otros organismos también depredadores, además, su producción está resguardada bajo secreto empresarial en la mayoría de los casos. Con esto, se abre la oportunidad para explorar especies nativas o incluso ya comercializadas, con el fin de evaluar su capacidad depredadora a nivel de investigación o como herramientas habituales para la regulación de plagas, un primer reto para este fin es la propagación y los métodos de cultivo. Los métodos para cultivar ácaros se pueden dividir en dos: mantenimiento sobre plantas y en confinamiento, aunque cada técnica se ha desarrollado para una especie en particular o un fin determinado, el conocer su diseño básico, promete acercarnos a una nueva línea de estudio para su introducción y reintroducción en los sistemas agrícolas.

ABSTRACT Due to the diversification of dairy products developed in the state of Falcón, the introduction of a fermented dairy product derived from yogurt combined with Aloe vera gel, a traditional crop of the region, is novel due to its nutritional properties and health benefits. The objective of this research was to evaluate the quality of a dairy drink with fermented aloe according to current regulations. Important authors such as Tamime and Robinson 1, Morillo and Toyo 2, Méndez and Romero 3 were taken as references. Product elaboration runs were carried out on a laboratory scale, in order to establish the adequate proportions of the fruity Aloe gel by mixing fruit pulp, sugar and Aloe gel, the most accepted being the 1:1:1 ratio. The physicochemical and microbiological characterization of the raw material and the finished product was carried out using the COVENIN standards and organoleptic tests to untrained judges to ensure the acceptance of the product, from which a fermented milk drink was obtained with the addition of 50 % gel of Fruity Aloe with a pH of 4,51; a titratable acidity of 0,837 % lactic acid; 3,64 % fat and 12,92 % non-fat solids. Regarding the microbiological quality, few yeasts and the absence of coliform bacteria were found, which complies with the physicochemical and microbiological requirements demanded by the regulations, allowing the obtaining of a quality product suitable for human consumption based on natural raw materials.

RESUMEN Debido a la diversificación de los productos de origen lácteo desarrollados en el estado Falcón, la introducción de un producto lácteo fermentado derivado del yogurt combinado con gel de Aloe vera, cultivo tradicional de la región, es novedoso por sus propiedades alimenticias y beneficios a la salud. Esta investigación tuvo como objetivo evaluar la calidad de una bebida láctea fermentada con gel de Aloe frutado según la normativa vigente. Se tomaron como referencias importantes autores como Tamime y Robinson, (1) Morillo y Toyo, (2) Méndez y Romero. (3) Se realizaron corridas de elaboración del producto a escala de laboratorio, a fin de establecer las proporciones adecuadas del gel de Aloe frutado mezclando pulpa de fruta, azúcar y gel de Aloe, siendo la más aceptada la proporción 1:1:1. Se realizó la caracterización fisicoquímica y microbiológica de la materia prima y del producto terminado mediante las normas COVENIN y pruebas organolépticas a 10 jueces no entrenados para asegurar la aceptación del producto, de donde se obtuvo una bebida láctea fermentada con la adición de 50 % de gel de Aloe frutado con un pH de 4,51; una acidez titulable de 0,837 % de ácido láctico; 3,64 % de grasa y 12,92 % de sólidos no grasos. Respecto a la calidad microbiológica se encontraron levaduras escasas y ausencia de bacterias coliformes, con lo cual se cumple con los requisitos fisicoquímicos y microbiológicos exigidos por la normativa, permitiendo la obtención de un producto de calidad apto para el consumo humano a base de materias primas naturales.

Abstract The effect of using alpaca milk on the physicochemical, composition, microbiological and sensory attributes of artisanal whipped yogurt with different alpaca and cow milk formulations was evaluated. The pasteurized process methodology to obtain seven formulations of yogurt with alpaca milk was subjected to physicochemical, composition, microbiological and sensory quality analysis with the following results: The physicochemical quality of yogurt made with different proportions of alpaca milk was favorable for the stability of the yogurt, also denoting a greater richness of total solids in its useful life period of consumption. The composition of yogurts with a different proportion of alpaca milk increases the nutritional components of yogurt, enriching it with natural nutrients, improving the quality of yogurt and promoting it as a “concentrated fermented milk.” The microbiological quality of yogurts made with various proportional parts of alpaca milk appears acceptable for consumption of yogurt according to the Peruvian Technical Standards for milk and dairy derivatives, given the absence of pathogenic microorganisms, minimal presentation of fungi and yeasts and presence initial acid lactobacilli, which inhibit bacterial growth and reduce bacterial presence at the time of final ripening of the yogurt. Finally, the sensory quality of the yogurts prepared with partial use of alpaca milk demonstrated to have good flavor, aroma and smell characteristics, mainly in those whose formulation uses majority proportions of alpaca milk, giving rise to products with acceptable tastes for the consumer.

Resumen Se evaluó el efecto del uso de leche de alpaca sobre los atributos fisicoquímicos, composición, microbiológico y sensorial del yogur batido artesanal con diferente formulación de leche de alpaca y vaca. La metodología de proceso pasteurizado para obtener siete formulaciones de yogurt con leche de alpaca, fueron sometidos al análisis fisicoquímico, composición, microbiológico y de calidad sensorial con resultados siguientes: La calidad fisicoquímica del yogurt elaborado con proporciones distintas de leche de alpaca resulto favorable para la estabilidad del yogurt, denotando también una mayor riqueza de solidos totales en su periodo de vida útil de consumo. La composición de los yogures con diversa proporción de leche de alpaca aumenta los componentes nutritivos del yogurt, enriqueciéndolo con nutrientes naturales, mejorando la calidad del yogurt y promoviéndola, como una “leche fermentada concentrada”. La calidad microbiológica de los yogures elaborados con partes proporcionales diversas de leche de alpaca, aparece aceptable para el consumo del yogurt según las Normas Técnicas Peruanas para la leche y derivados lácteos, dada la ausencia de microorganismos patógenos, mínima presentación de hongos y levaduras y presencia inicial de lactobacilos ácidos, que inhiben el crecimiento bacteriano y disminuyen presencia bacteriana al momento de maduración final del yogurt. Finalmente, la calidad sensorial de los yogures preparados con uso parcial de leche de alpaca demostró tener buenas características de sabor aroma y olor, principalmente en aquellos cuya formulación hace uso de proporciones mayoritarias de leche de alpaca, dando lugar a productos de gustos aceptables para el consumo.

RESUMEN Antecedentes: La biorrefinería marina es un concepto emergente en la industria que busca maximizar la utilización de los recursos naturales a través de procesos sostenibles y eficientes. Esta se enfoca en el aprovechamiento de recursos marinos en respuesta a la necesidad de encontrar alternativas a los procesos industriales tradicionales que suelen generar grandes cantidades de residuos y consumir recursos de manera no renovable. Objetivo: Realizar una revisión de la literatura científica sobre la importancia de los productos derivados de los desperdicios de la industria acuícola, con un enfoque de biorrefinería en el contexto de la realidad cubana. Desarrollo: Se realizó una búsqueda de información en la literatura científica especializada; sintetizándose lo más importante, se logró definir el concepto de biorrefinería, basado en la utilización de desechos de la industria acuícola, los productos que se obtienen de la misma, así como sus principales características y aplicaciones y se planteó la importancia y ventajas de aplicar este concepto a la realidad cubana. Conclusiones: La biorrefinería a partir de desechos de la industria pesquera, es un enfoque innovador que busca aprovechar al máximo todos sus componentes, incluidos los subproductos y desechos generados, esta estrategia está muy relacionada con los principios de economía circular y promueve la sostenibilidad de la industria.

ABSTRACT Background: The marine biorefinery is an emerging concept in the industry that seeks to maximize the use of natural resources through sustainable and efficient processes. This focuses on the use of marine resources in response to the need to find alternatives to traditional industrial processes that usually generate large amounts of waste and consume resources in a non-renewable way. Aim: Conduct a review of the scientific literature on the importance of products derived from waste from the aquaculture industry, with a biorefinery approach in the context of the Cuban reality. Development: A search for information was carried out in the specialized scientific literature; Synthesizing the most important thing, it was possible to define the concept of biorefinery, based on the use of waste from the aquaculture industry, the products obtained from it, as well as its main characteristics and applications and the importance and advantages of applying this were raised. concept to the Cuban reality. Conclusions: The biorefinery using waste from the fishing industry is an innovative approach that seeks to make the most of all its components, including the by-products and waste generated. This strategy is closely related to the principles of circular economy and promotes the sustainability of the industry.

Abstract Introduction: Care towards nutrition is essential for the quality of a sustainable aquaculture product. Since the balance in food affects the growth and production of gametes. The circular economy is made possible through the use of discarded materials. Objective: The aim of this research was to study the fatty acid composition and metabolic pathways in the gametes of Arbacia dufresnii, with a focus on the implications of incorporating shrimp byproducts into aquaculture feeds. Methods: Four different treatments were designed to maintain optimal nutritional quality, particularly in lipids and proteins, based on previous studies. The fatty acid profiles of the feeds and gametes were analyzed by using gas-chromatography, and statistical analyses were conducted to determine significant differences. Results: Significant differences were observed in the abundance (%) of omega-3 (ω-3) and omega-6 (ω-6) fatty acids. The (ω-3) metabolic pathway was more pronounced in the gametes of wild animals and those fed with the experimental feeds. In contrast, the (ω-6) metabolic pathway was less relevant in these groups. The (ω-3) /(ω-6) ratio was highest in the gametes of wild animals. Feeds enriched in fatty acids enhanced their bioaccumulation in the gametes reaching higher concentrations than wild animals. The availability of fatty acids in foods allowed their bioaccumulation in gametes, with concentrations equal to or higher than those observed in animals in their natural environment for certain fatty acids. Conclusions: Incorporating shrimp byproducts in aquaculture feeds demonstrated a promising strategy for resource utilization and organic input generation. The fatty acid composition in the gametes of A. dufresnii was influenced by the diet, highlighting the potential of balanced feeds to enhance the bioaccumulation of essential fatty acids. These findings provide valuable insights for the development of sustainable aquaculture practices and the production of nutritionally enriched seafood products.

Resumen Introducción: El cuidado hacia la nutrición es fundamental para la calidad de un producto acuícola sostenible. Ya que el balance en los alimentos afecta el crecimiento y producción de los gametos. A partir del aprovechamiento de materias de descarte se posibilita la economía circular. Objetivo: El objetivo de este estudio fue investigar la composición de ácidos grasos y las vías metabólicas en los gametos de Arbacia dufresnii, centrándose en las implicaciones de la incorporación de subproductos de camarones en los alimentos de acuicultura. Métodos: Se diseñaron cuatro tratamientos diferentes para mantener una calidad nutricional óptima, especialmente en lípidos y proteínas, basándose en estudios previos. Se analizaron los perfiles de ácidos grasos de los alimentos y los gametos mediante cromatografía de gases, y se realizaron análisis estadísticos para determinar diferencias significativas. Resultados: Se observaron diferencias significativas en la abundancia (%) de ácidos grasos omega-3 (ω-3) y omega-6 (ω-6). La vía metabólica de (ω-3) fue más pronunciada en los gametos de los animales en su entorno natural y aquellos alimentados con los piensos experimentales. Por el contrario, la vía metabólica de (ω-6) tuvo menos relevancia en estos grupos. La relación (ω-3) /(ω-6) fue más alta en los gametos de los animales en su entorno natural. La disponibilidad de ácidos grasos en los alimentos permitió su bioacumulación en los gametos, con concentraciones iguales o superiores a las observadas en los animales en su entorno natural para ciertos ácidos grasos. Conclusiones: La incorporación de subproductos de camarones en los alimentos de acuicultura demostró ser una estrategia prometedora para la utilización de recursos y la generación de insumos orgánicos. La composición de ácidos grasos en los gametos de A. dufresnii fue influenciada por la dieta, destacando el potencial de los alimentos balanceados para mejorar la bioacumulación de ácidos grasos esenciales. Estos hallazgos brindan información valiosa para el desarrollo de prácticas sostenibles en acuicultura y la producción de productos marinos enriquecidos nutricionalmente.