This study aims to propose a new method for derivatizing fatty acids (FAs) from human milk (HM), eliminating the lipid extraction step, and simplifying the preparation for gas chromatography with a flame ionization detection (GC-FID) analysis to quantify the FAs. The Design Expert software optimized the reaction times, concentrations, and sample amount. The proposed method (PM) was validated for lyophilized HM and results for the figures of merit for precision in relative standard deviation (RSD) (RSDintra-day 1.34-4.03% and RSDinter day 2.08 5.16%), accuracy (99.87-102.16%), and robustness are within a linear range of 3 to 38% lipids in HM samples. The atmospheric solids analysis probe tandem mass spectrometry (ASAP-MS/MS) technique confirmed the efficiency of PM by expressing the molecular composition of triacylglycerol formed by FAs from the GC-FID technique. The PM requires a small sample size and conducts derivatization directly in the sample matrix, minimizing extraction errors. (FAs HM, , (HM) step GCFID GC FID (GC-FID times concentrations amount (PM RSD (RSD RSDintraday RSDintra 1.344.03% 134403 1.34 4.03% 1 34 4 03 1.34-4.03 208 2 08 2.0 5.16%, 516 5.16% 5 16 5.16%) 99.87102.16%, 998710216 99.87 102.16% 99 87 102 (99.87-102.16%) 38 samples ASAPMS/MS ASAPMSMS ASAP MS/MS MS (ASAP-MS/MS matrix errors (HM 344 1.344.03 13440 134 1.3 403 4.03 0 1.34-4.0 20 2. 51 5.16 87102 99.87102.16% 99871021 9987 99.8 10216 102.16 9 8 10 (99.87-102.16% ASAPMS MSMS 1.344.0 1344 13 1. 40 4.0 1.34-4. 5.1 8710 99.87102.16 9987102 998 99. 1021 102.1 (99.87-102.16 1.344. 4. 1.34-4 5. 871 99.87102.1 998710 102. (99.87-102.1 1.344 1.34- 99.87102. 99871 (99.87-102. 99.87102 (99.87-102 99.8710 (99.87-10 99.871 (99.87-1 (99.87- (99.87 (99.8 (99. (99 (9 (

Exploring the triacylglycerols composition in milk samples from different ruminant species is crucial for dairy product development. Identifying the fatty acid (FA) composition in human milk is essential and is usually performed using gas chromatography (GC). Heat maps are useful for visualizing these results, aiding in pattern identification and relationships between samples and variables. Additionally, nuclear magnetic resonance (NMR) spectroscopy provides important qualitative and quantitative information about fatty acids (FA). In the present study, we compare the fatty acid composition in samples of HM, cow milk, and goat milk, as well as to demonstrate their positional distribution in triacylglycerols (TAGs). Human milk contains 49.21% saturated fatty acids (SFAs), 34.32% monounsaturated fatty acids (MUFA), and 16.47% polyunsaturated fatty acids (PUFA), including 2.14% docosahexaenoic acid (DHA) and 13.02% linoleic acid (LA). On the other hand, cow and goat milk have higher proportions of SFAs (56.2 and 58.02%, respectively) and MUFAs (40.56 and 40.95%, respectively), but lower amounts of PUFAs (3.24 and 1.03%, respectively), with significantly lower quantities of DHA (0.03 and 0.43%, respectively) and LA (1.46 and 2.76%, respectively). Additionally, fatty acids in ruminant milk mainly consist of long-chain fatty acids (LCFAs), with oleic acid being the most abundant. development FA (FA GC. GC . (GC) results variables Additionally NMR (NMR FA. study HM TAGs. TAGs (TAGs) 4921 49 21 49.21 SFAs, , (SFAs) 3432 34 32 34.32 MUFA, MUFA (MUFA) 1647 16 47 16.47 PUFA, PUFA (PUFA) 214 2 14 2.14 (DHA 1302 13 02 13.02 LA. (LA) hand 56.2 562 56 (56. 5802 58 58.02% respectively 40.56 4056 40 (40.5 4095 95 40.95% respectively, 3.24 324 3 24 (3.2 103 1 03 1.03% 0.03 003 0 (0.0 043 43 0.43% 1.46 146 46 (1.4 276 76 2.76% respectively. longchain long chain LCFAs, LCFAs (LCFAs) abundant (GC (TAGs 492 4 49.2 (SFAs 343 34.3 (MUFA 164 16.4 (PUFA 2.1 130 13.0 (LA 56. 5 (56 580 58.02 40.5 405 (40. 409 9 40.95 3.2 (3. 10 1.03 0.0 00 (0. 04 0.43 1.4 (1. 27 7 2.76 (LCFAs 49. 34. 16. 2. 13. (5 58.0 40. (40 40.9 3. (3 1.0 0. (0 0.4 1. (1 2.7 ( 58. (4

RESUMO Objetivo: Comparar a presença de anticorpos neutralizantes contra o SARS-CoV-2 no leite materno e no sangue das lactantes vacinadas em relação àquelas não vacinadas. Fontes de dados: Foi efetuado registro no International Prospective Register of Systematic Reviews — PROSPERO (CRD42021287554) e foram seguidas as diretrizes do Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA). Foram elegíveis estudos de coorte, caso-controle e transversais que avaliaram anticorpos contra o SARS-CoV-2 no leite e no sangue de lactantes vacinadas e tiveram como grupo controle lactantes não vacinadas. Utilizaram-se os Descritores em Ciências da Saúde (DeCs), Medical Subject Heading (MeSH) e Emtree para as bases Biblioteca Virtual em Saúde (BVS), Medical Literature Analysis and Retrieval System Online (Medline/PubMed) e Embase, respectivamente. Na Web of Science e Scopus foi feita adaptação da estratégia. Não foram estabelecid as restrições quanto ao período de publicação e idioma. Síntese dos dados: As buscas identificaram 233 registros. Foram excluídos 128 duplicados e 101 fora dos critérios de inclusão, e quatro estudos de coorte foram elegíveis. As lactantes vacinadas com Pfizer-BioNTech e Moderna apresentaram anticorpos contra SARS-CoV-2 no sangue e no leite materno. Conclusões: As mulheres lactantes vacinadas apresentaram níveis mais elevados de imunoglobulina G (IgG) e A (IgA) no soro e no leite materno em comparação com as mulheres não vacinadas. Objetivo SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- dados CRD42021287554 CRD (CRD42021287554 MetaAnalysis Meta PRISMA. PRISMA . (PRISMA) casocontrole caso Utilizaramse Utilizaram se DeCs, DeCs , (DeCs) MeSH (MeSH BVS, BVS (BVS) Medline/PubMed MedlinePubMed Medline PubMed (Medline/PubMed Embase respectivamente estratégia idioma 23 registros 12 10 inclusão PfizerBioNTech Pfizer BioNTech Conclusões IgG (IgG IgA (IgA SARS-CoV CRD4202128755 (CRD4202128755 (PRISMA (DeCs (BVS 1 CRD420212875 (CRD420212875 CRD42021287 (CRD42021287 CRD4202128 (CRD4202128 CRD420212 (CRD420212 CRD42021 (CRD42021 CRD4202 (CRD4202 CRD420 (CRD420 CRD42 (CRD42 CRD4 (CRD4 (CRD

ABSTRACT Objective: To compare the presence of neutralizing antibodies against SARS-CoV-2 found in the breast milk and blood of vaccinated lactating women with those not vaccinated. Data source: The study was registered in the International Prospective Register of Systematic Reviews (PROSPERO) under CRD42021287554 and followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Cohort, case-control, and cross-sectional studies that evaluated antibodies against SARS-CoV-2 in the milk and blood of vaccinated mothers and had as control group unvaccinated mothers were eligible. Health Sciences Descriptors (DeCs), Medical Subject Headings (MeSH) and Emtree descriptors were used for the Virtual Health Library (VHL), Medical Literature Analysis and Retrieval System Online (Medline/Pubmed), and Embase databases, respectively. In the Web of Science and Scopus, the strategy was adapted. No restrictions on the publication period and language were set. Data synthesis: The search identified 233 records, of which 128 duplicates and 101 papers that did not meet the inclusion criteria were excluded. Hence, four cohort studies were eligible. Nursing mothers vaccinated with the Pfizer-BioNTech and Moderna vaccines showed antibodies against SARS-CoV-2 in their blood and breast milk. Conclusions: Vaccinated lactating women had higher levels of immunoglobulin G (IgG) and A (IgA) in serum and breast milk than unvaccinated women. Objective SARSCoV2 SARSCoV SARS CoV 2 SARS-CoV- source PROSPERO (PROSPERO CRD CRD4202128755 MetaAnalyses Meta Analyses PRISMA (PRISMA guidelines Cohort casecontrol, casecontrol case control, case-control crosssectional cross sectional eligible DeCs, DeCs , (DeCs) MeSH (MeSH VHL, VHL (VHL) Medline/Pubmed, MedlinePubmed Medline/Pubmed Medline Pubmed (Medline/Pubmed) databases respectively Scopus adapted set synthesis 23 records 12 10 excluded Hence PfizerBioNTech Pfizer BioNTech Conclusions IgG (IgG IgA (IgA SARS-CoV CRD420212875 (DeCs (VHL (Medline/Pubmed 1 CRD42021287 CRD4202128 CRD420212 CRD42021 CRD4202 CRD420 CRD42 CRD4

Caffeine (CAF) and nicotine (NIC) are emerging contaminants and are among the most consumed substances in the world, making it crucial to monitor these contaminants. In this work, an alternative electrolyte, 2-hydroxyethylammonium acetate (2HEAA) was used for the development of an electroanalytical method for determination and quantification of CAF and NIC, simultaneously, using differential pulse voltammetry (DPV). The system with the 2HEAA electrolyte was characterized by cyclic voltammetry and electrochemical impedance spectroscopy, and the DPV parameters were optimized for the best conditions. The method was validated from a calibration curve obtained which showed limit of detection (LOD) of 0.82 and 6.26 μmol L 1 and limit of quantification (LOQ) of 2.73 and 20.8 μmol L-1 for CAF and NIC, respectively. In the precision analyses, values lower than 10% of relative standard deviation were obtained. In the presence of concomitant inorganic and organic species, the system proved to be selective in the determination of analytes. The method was used to determine the analytes in fortified samples (river water, synthetic urine and commercial milk), obtaining recoveries between 87.25 and 111.40%. The 2HEAA demonstrated high efficiency as an alternative electrolyte with good signal-to-noise ratio, increased analytical sensitivity of the method, in addition to presenting low cost and fast electrolyte preparation. (CAF NIC (NIC world work 2hydroxyethylammonium hydroxyethylammonium 2 HEAA (2HEAA simultaneously DPV. . (DPV) spectroscopy conditions LOD (LOD 082 0 82 0.8 626 6 26 6.2 LOQ (LOQ 273 73 2.7 208 20 8 20. L1 L- respectively analyses 10 species river water milk, milk , milk) 8725 87 25 87.2 11140 111 40 111.40% signaltonoise signal noise ratio preparation (DPV 08 0. 62 6. 27 7 2. 872 87. 1114 11 4 111.40 111.4 111.

RESUMO: A fisiologia das plantas muda ao longo do dia porque os fotoassimilados podem aumentar a concentração de carboidratos solúveis em água à tarde em comparação com a manhã. Este estudo teve como objetivo avaliar o efeito do horário de colheita na composição morfológica da silagem de milho de planta inteira, tamanho de partícula, perfil de fermentação, composição química, degradação in vitro e estimativa da produção de leite. Um ensaio agronômico de dois anos foi realizado em delineamento inteiramente casualizado, e um silo experimental foi produzido por cada parcela (n = 16). A colheita da tarde aumentou (P < 0,01) o teor de matéria seca em relação à colheita da manhã. A época de colheita não afetou (P ≥ 0,32) a proporção de grãos, colmos e folhas do milho e o tamanho das partículas da silagem. Entretanto, a colheita da manhã aumentou (P = 0,01) a recuperação de matéria seca e não teve efeito (P ≥ 0,10) no pH da silagem e nas concentrações de ácido láctico e acético em comparação à colheita da tarde. Além disso, a colheita da tarde, em vez da colheita da manhã, aumentou (P ≤ 0,05) o amido da silagem, os carboidratos solúveis em água, a lignina detergente ácida e o teor de extrato etéreo e a degradação in vitro da matéria seca. Controversamente, os tratamentos não mostraram efeito (P ≥ 0,14) na concentração de energia da silagem, no conteúdo energético estimado e na produção de leite. Assim, a colheita da manhã produz mais matéria seca da silagem, mas a colheita da tarde melhora o valor nutricional da silagem de milho sem impacto na produção estimada de leite. RESUMO inteira partícula fermentação química leite casualizado n 16. 16 . 16) P 0,01 001 0 01 0,32 032 32 grãos Entretanto 0,10 010 10 disso 0,05 005 05 Controversamente 0,14 014 14 Assim 1 0,0 00 0,3 03 3 0,1 0,

ABSTRACT: Plants’ physiology changes through the day because photoassimilates can increase water-soluble carbohydrate concentration in the afternoon compared to the morning. This study evaluated the harvest time effect on whole-plant corn silage morphological composition, particle size, fermentation profile, chemical composition, in vitro degradation, and estimated milk yield. A two-year agronomic assay was performed in a completely random design, and one experimental silo was produced by each parcel (n = 16). The afternoon harvest increased (P < 0.01) dry matter content compared to the morning harvest. Harvest time did not affect (P ≥ 0.32) corn grain, stalk, leaf proportion, and silage particle size. However, the morning harvest increased dry matter recovery (P = 0.01) and had no effect (P ≥ 0.10) on silage pH and concentrations of lactic and acetic acid compared to the afternoon harvest. In addition, afternoon harvest instead of morning harvest increased (P ≤ 0.05) silage starch, water-soluble carbohydrates, acid detergent lignin, and ether extract content and dry matter in vitro degradation. Controversially, treatments showed no effect (P ≥ 0.14) on silage energy concentration, estimated energy content, and milk yield. Thus, the morning harvest produces more silage dry matter, but the afternoon harvest improves corn silage’s nutritional value with no impact on estimated milk yield. ABSTRACT Plants watersoluble water soluble wholeplant whole plant composition size profile degradation yield twoyear two year design n 16. 16 . 16) P 0.01 001 0 01 0.32 032 32 grain stalk proportion However 0.10 010 10 addition 0.05 005 05 starch carbohydrates lignin Controversially 0.14 014 14 Thus silages s 1 0.0 00 0.3 03 3 0.1 0.

RESUMO: A mastite bovina é a doença mais impactante na indústria de laticínios e é caracterizada por uma complexidade de agentes causais, os quais têm revelado uma variação geográfica entre regiões e países. Os patógenos relacionados à mastite têm sido tradicionalmente classificados como contagiosos ou ambientais, com base na fonte de infecção ou origem e nas vias de transmissão. Além disso, a gravidade das infecções mamárias tem sido associada à virulência dos patógenos, e ao estado imunológico e nutricional dos animais. Considerando esse cenário, investigamos os agentes etiológicos, os escores de gravidade clínica e os dados de dias em lactação (DEL) em 4.273 casos clínicos de mastite bovina em dez fazendas de grande porte, quatro localizadas no sudoeste do estado de São Paulo e seis no sul do Estado de Minas Gerais, Brasil. Streptococcus dysgalactiae (283/4.273 = 6,6%), Escherichia coli (190/4.273 = 4,4%), Prototheca spp. (112/4.273 = 2,6%) e Streptococcus uberis (95/4.273 = 2,2%) foram os patógenos predominantes isolados. Entre os 4.273 casos clínicos, a pontuação de gravidade clínica estava disponível em 43,8% (1.871/4.273) dos animais. Desses, 69,8% (1.306/1.871), 27,3% (510/1.871) e 2,9% (55/1.871) foram classificados como leves, moderados e graves, respectivamente. Os patógenos foram observados principalmente em amostras obtidas durante os primeiros 100 dias em lactação, e a gravidade clínica foi principalmente classificada como leve. Nossos resultados contribuem para a identificação etiológica, a pontuação de gravidade clínica e os aspectos de ordenha de vacas com mastite em fazendas leiteiras com histórico de infecções mamárias clínicas. RESUMO causais países ambientais transmissão disso animais cenário etiológicos DEL (DEL 4273 4 273 4.27 porte Gerais Brasil 283/4.273 2834273 283 (283/4.27 6,6%, 66 6,6% , 6 6,6%) 190/4.273 1904273 190 (190/4.27 4,4%, 44 4,4% 4,4%) spp 112/4.273 1124273 112 (112/4.27 2,6% 26 2 95/4.273 954273 95 (95/4.27 2,2% 22 isolados 438 43 8 43,8 1.871/4.273 18714273 1 871 (1.871/4.273 Desses 698 69 69,8 1.306/1.871, 13061871 1.306/1.871 306 (1.306/1.871) 27 3 27,3 510/1.871 5101871 510 (510/1.871 29 9 2,9 55/1.871 551871 55 (55/1.871 leves graves respectivamente 10 leve etiológica clínicas 427 4.2 283/4.27 283427 28 (283/4.2 6,6 190/4.27 190427 19 (190/4.2 4,4 112/4.27 112427 11 (112/4.2 2,6 95/4.27 95427 (95/4.2 2,2 43, 1.871/4.27 1871427 87 (1.871/4.27 69, 1306187 1.306/1.87 30 (1.306/1.871 27, 510/1.87 510187 51 (510/1.87 2, 55/1.87 55187 5 (55/1.87 42 4. 283/4.2 28342 (283/4. 6, 190/4.2 19042 (190/4. 4, 112/4.2 11242 (112/4. 95/4.2 9542 (95/4. 1.871/4.2 187142 (1.871/4.2 130618 1.306/1.8 (1.306/1.87 510/1.8 51018 (510/1.8 55/1.8 5518 (55/1.8 283/4. 2834 (283/4 190/4. 1904 (190/4 112/4. 1124 (112/4 95/4. 954 (95/4 1.871/4. 18714 (1.871/4. 13061 1.306/1. (1.306/1.8 510/1. 5101 (510/1. 55/1. 551 (55/1. 283/4 (283/ 190/4 (190/ 112/4 (112/ 95/4 (95/ 1.871/4 1871 (1.871/4 1306 1.306/1 (1.306/1. 510/1 (510/1 55/1 (55/1 283/ (283 190/ (190 112/ (112 95/ (95 1.871/ 187 (1.871/ 130 1.306/ (1.306/1 510/ (510/ 55/ (55/ (28 (19 (11 (9 1.871 18 (1.871 13 1.306 (1.306/ (510 (55 (2 (1 ( 1.87 (1.87 1.30 (1.306 (51 (5 1.8 (1.8 1.3 (1.30 1. (1. (1.3

ABSTRACT: Bovine mastitis is the most impacting disease of dairy industry, and it is characterized by a complexity of causal agents, which have revealed a geographical variation among regions and countries. The mastitis-related pathogens have been traditionally classified as contagious or environmental, based on habits of the microorganisms and transmission routes. Besides, the severity of mammary infections has been associated with the virulence of the pathogens, and immune and nutritional status of the hosts. Considering this scenario, we investigated the etiological pattern, clinical severity scores, and days in milk (DIM) data in 4,273 clinical cases of bovine mastitis among ten large-dairy farms located in Brazil. Streptococcus dysgalactiae (283/4,273 = 6.6%), Escherichia coli (190/4,273 = 4.4%), Prototheca spp. (112/4,273 = 2.6%), and Streptococcus uberis (95/4,273 = 2.2%) were the predominant pathogens isolated. Among 4,273 clinical cases, clinical severity score was available in 43.8% (1,871/4,273) animals. From these, 69.8% (1,306/1,871), 27.3% (510/1,871), and 2.9% (55/1,871) were scored as mild, moderate, and severe, respectively. Pathogens were observed mainly in samples obtained during the first 100 days in milk, and clinical severity scored mainly as mild. Our results contributed to the etiological identification, clinical severity scoring, and milking aspects of mastitic cows in dairy farms with a history of clinical mammary infections. ABSTRACT industry agents countries mastitisrelated related environmental routes Besides hosts scenario pattern scores DIM (DIM 4273 4 273 4,27 largedairy large Brazil 283/4,273 2834273 283 (283/4,27 6.6%, 66 6.6% , 6 6.6%) 190/4,273 1904273 190 (190/4,27 4.4%, 44 4.4% 4.4%) spp 112/4,273 1124273 112 (112/4,27 2.6%, 26 2.6% 2 2.6%) 95/4,273 954273 95 (95/4,27 2.2% 22 isolated 438 43 8 43.8 1,871/4,273 18714273 1 871 (1,871/4,273 animals these 698 69 69.8 1,306/1,871, 13061871 1,306/1,871 306 (1,306/1,871) 27 3 27.3 510/1,871, 5101871 510/1,871 510 (510/1,871) 29 9 2.9 55/1,871 551871 55 (55/1,871 mild moderate severe respectively 10 identification scoring 427 4,2 283/4,27 283427 28 (283/4,2 6.6 190/4,27 190427 19 (190/4,2 4.4 112/4,27 112427 11 (112/4,2 2.6 95/4,27 95427 (95/4,2 2.2 43. 1,871/4,27 1871427 87 (1,871/4,27 69. 1306187 1,306/1,87 30 (1,306/1,871 27. 510187 510/1,87 51 (510/1,871 2. 55/1,87 55187 5 (55/1,87 42 4, 283/4,2 28342 (283/4, 6. 190/4,2 19042 (190/4, 4. 112/4,2 11242 (112/4, 95/4,2 9542 (95/4, 1,871/4,2 187142 (1,871/4,2 130618 1,306/1,8 (1,306/1,87 51018 510/1,8 (510/1,87 55/1,8 5518 (55/1,8 283/4, 2834 (283/4 190/4, 1904 (190/4 112/4, 1124 (112/4 95/4, 954 (95/4 1,871/4, 18714 (1,871/4, 13061 1,306/1, (1,306/1,8 5101 510/1, (510/1,8 55/1, 551 (55/1, 283/4 (283/ 190/4 (190/ 112/4 (112/ 95/4 (95/ 1,871/4 1871 (1,871/4 1306 1,306/1 (1,306/1, 510/1 (510/1, 55/1 (55/1 283/ (283 190/ (190 112/ (112 95/ (95 1,871/ 187 (1,871/ 130 1,306/ (1,306/1 510/ (510/1 55/ (55/ (28 (19 (11 (9 1,871 18 (1,871 13 1,306 (1,306/ (510/ (55 (2 (1 ( 1,87 (1,87 1,30 (1,306 (510 (5 1,8 (1,8 1,3 (1,30 (51 1, (1, (1,3

RESUMEN Introducción: La duración de la lactancia materna es crucial para la salud infantil, sin embargo, la prevalencia ha disminuido en los últimos años, situando a los niños en una población de riesgo. Objetivo: Determinar los factores asociados a la duración de la lactancia materna en niños menores de 2 años en el Perú 2021-2022. Metodología: Estudio analítico transversal, a través del análisis secundario de datos de la Encuesta Demográfica y de Salud Familiar (ENDES) del año 2021 y 2022. El tamaño muestral fue de 4951 niños/niñas con sus respectivas madres. Se evaluaron factores infantiles y maternos utilizando el Estadístico F corregida para el análisis bivariado con una significancia estadística p<0,05 y un intervalo de confianza al 95%. Finalmente, para medir la asociación se calculó la razón de prevalencia cruda (RPc) y la razón de prevalencia ajustada (RPa) mediante la Regresión de Poisson con varianza robusta. Resultados: El 85,3% tuvo una duración de 0-6 meses y solo el 14,7% tiene una duración hasta los 23 meses. El no contacto piel a piel precoz bebe-madre (p <0,01) (RPa 1,19) y el consumo de bebidas diferentes a la leche materna los primeros 3 días de nacido (p <0,01) (RPa 0,66) se asociaron con una duración hasta los 6 meses de lactancia materna. Las madres adolescentes tuvieron un 30,7% de duración de lactancia materna de 6 meses (p<0,01) (RPa 2,49), por el contrario, el lugar de residencia rural, el grado no superior y un menor ingreso económico permiten una lactancia materna por más de 6 meses. Discusión: Los resultados asocian no recibir contacto piel a piel precoz bebe-madre, edad materna igual o menor de 18 años, grado superior, lugar de residencia urbano y mayor ingreso económico con una menor duración de lactancia materna en niños menores de 2 años.

ABSTRACT Introduction: The duration of breastfeeding is crucial for child health; however, the prevalence has decreased in recent years, placing children in a population at risk. Objective: To determine the factors associated with the duration of breastfeeding in children under 2 years of age in Peru 2021-2022. Methodology: Cross-sectional analytical study, through the secondary analysis of data from the Demographic and Family Health Survey (ENDES) of 2021 and 2022. The sample size was 4951 children with their respective mothers. Infant and maternal factors were evaluated using the F-statistic corrected for bivariate analysis with a statistical significance of p<0.05 and a 95% confidence interval. Finally, to measure the association, the crude prevalence ratio (cPR) and the adjusted prevalence ratio (aPR) were calculated using Poisson regression with robust variance. Results: 85.3% had a duration of 0-6 months and only 14.7% had a duration of up to 23 months. Early baby-mother skin-to-skin non-contact (p <0.01) (aPR 1.19) and consumption of beverages other than breast milk in the first 3 days of birth (p <0.01) (aPR 0.66) were associated with a duration of breastfeeding up to 6 months. Adolescent mothers had a 30.7% duration of breastfeeding of 6 months (p<0.01) (aPR 2.49), on the contrary, the place of rural residence, the non-higher grade and a lower economic income allow breastfeeding for more than 6 months. Discussion: The results were associated with not receiving early skin-to-skin contact between the baby and the mother, maternal age equal to or less than 18 years, higher grade, urban place of residence and higher economic income with a shorter duration of breastfeeding in children under 2 years of age.

Resumen Listeria monocytogenes es una bacteria aeróbica Gram positiva; encontrada enforma ubicua en la naturaleza; que afecta sobre todo a recién nacidos, adultos mayores, pacientes inmunodeprimidos y mujeres embarazadas. Sin embargo, la enfermedad por Listeria puede ocurrir en la población sana. La listeriosis invasiva posee 3 formas clínicas do minantes, bacteriemia, neurolisteriosis e infección materno-neonatal. Las formas localizadas se describen infrecuentemente. La enfermedad se produce fun damentalmente en forma secundaria al consumo de alimentos contaminados, incluidos leche o queso no pasteurizados, y sepresenta en forma de casos aislados o brotes, soliendo comenzar a los pocos días del con sumo de éstos; aunque se ha descripto hasta 2 meses después de ingerirlos. También existela posibilidad de transmisión directa desde animales y transmisión vertical. La listeriosis sistémica sin cuadro neurológico do minante es un evento raro. Se presentan dos casos. El primero, una espondilodiscitis en huésped normal y el segundo una bacteriemia por Listeria en un paciente inmunocomprometido febril.

Abstract Listeria monocytogenes is a Gram-positive aerobic bac terium; found ubiquitously in nature; which mainly affects newborns, older adults, immunosuppressed pa tients and pregnant women. However, Listeria disease can occur in the healthy population. Invasive listeriosis has three dominant clinical forms, bacteremia, neurolisteriosis and maternal-neonatal in fection. Localized forms are infrequently described. The disease occurs mainly secondary to the consumption of contaminated food, including unpasteurized milk or cheese, and occurs in the form of isolated cases or outbreaks, usually beginning a few days after consump tion of the contaminated food; although it has been described up to 2 months after ingesting them. There is also the possibility of direct transmission from animals and vertical transmission. Systemic listeriosis without dominant neurological symp toms is a rare event. Two cases are presented. The first was spondylodiscitis in a normal host and the second was Listeria bacteremia in a febrile immunocompromised patient.

Abstract In Tamaulipas extensive goat production systems in the semi-arid region of the Altiplano contribute annually with the production of 41% of live animals and 87% of milk in the state. The objective of the study was to characterize the goat production units in the municipalities of Jaumave, Bustamante and Tula, in which a significant number of production units are located. Through the application of structured surveys conducted in December 2021 and January 2022, socioeconomic, nutritional, productive, reproductive, and sanitary information was obtained from 40 goat production units. Data was analyzed using principal component factor analysis and a cluster analysis with the Statistical Program for Social Sciences (SPSS version 24). Four factors (management, production, grazing and reproduction) explained most of the total variation of the data and four groups of production units were determined. The characterization of the extensive goat production system in the Altiplano of Tamaulipas is described in general terms with management and production variables. However, the analysis of the typologies of production units showed heterogeneity in the system, in this sense, the differences between the means of the groupings that stood out the most were zootechnical purpose, reproduction, production and annual income. Underfeeding, scarce water sources and poor reproductive management are associated with low productivity and high animal mortality.

Resumen En Tamaulipas la ganadería caprina extensiva de la región semiárida del Altiplano contribuye anualmente con la producción del 41% de ganado en pie y el 87% de leche en el estado. El objetivo de la investigación fue caracterizar el sistema extensivo que practican las unidades de producción caprinas de los municipios de Jaumave, Bustamante y Tula. Mediante la aplicación de encuestas estructuradas que se realizaron en meses de diciembre 2021 y enero 2022 se obtuvo información socioeconómica, alimenticia, productiva, reproductiva y sanitaria de 40 unidades de producción caprinas. Para sintetizar y tipificar la información se realizó un análisis factorial de componentes principales y un análisis clúster mediante el Programa Estadístico de Ciencias Sociales (SPSS versión 24). Se identificaron cuatro factores (manejo, producción, pastoreo y reproducción) que explican la mayor parte de la variabilidad total de los datos y se determinaron cuatro grupos de unidades de producción. La caracterización del sistema caprino de producción extensiva en el Altiplano de Tamaulipas se describe de forma general con variables de manejo y producción. Sin embargo, el análisis de las tipologías de unidades de producción mostró heterogeneidad en el sistema, en este sentido, las diferencias entre las medias de las agrupaciones que más destacaron fueron finalidad zootécnica, reproducción, producción e ingresos anuales. La subalimentación, escasez de agua y deficiente manejo reproductivo se asocian a la baja productividad y alta mortalidad de los animales.

Abstract This paper involves the analysis of the quality of anthropometric data on children under five years of age in two information systems in the State of São Paulo. The sample included 2,117,108 children from the Food and Nutrition Surveillance System (SISVAN), and 748,551 from the State Milk Project (VIVALEITE). Initially, we evaluated the frequency of missing values and others outside the equipment spectrum and calculated the digit-to-weight preference index. After calculating height-for-age (HAZ), weight-for-age (WAZ), and body mass index-for-age (BAZ), we flagged the biologically implausible values (BIV) and calculated the standard deviation (SD). For each municipality, we calculated the mean and the SD of HAZ, WAZ, and BAZ; and plotted the SD values as a function of the mean. The digit-to-weight preference index was greater among children aged between 24 and 59 months in SISVAN. The frequency of BIV for HAZ (SISVAN 2.56%; VIVALEITE 0.98%) was higher than for WAZ (SISVAN 2.10%; VIVALEITE 0.18%). For HAZ, variations among municipalities were more pronounced in VIVALEITE than in SISVAN. The height variable presents low reliability in both systems. The weight variable reveals satisfactory quality in VIVALEITE and unsatisfactory quality in SISVAN. Paulo 2117108 2 117 108 2,117,10 SISVAN, SISVAN , (SISVAN) 748551 748 551 748,55 VIVALEITE. . (VIVALEITE) Initially digittoweight digit to heightforage (HAZ) weightforage (WAZ) indexforage BAZ, BAZ (BAZ) (BIV SD. (SD) municipality 5 2.56% 256 56 0.98% 098 0 98 2.10% 210 10 0.18%. 018 0.18% 18 0.18%) 211710 11 2,117,1 74855 74 55 748,5 (VIVALEITE (HAZ (WAZ (BAZ (SD 2.56 25 0.98 09 9 2.10 21 1 01 0.18 21171 2,117, 7485 7 748, 2.5 0.9 2.1 0.1 2117 2,117 2. 0. 211 2,11 2,1 2,

Resumo O objetivo foi analisar a qualidade dos dados antropométricos de crianças menores de cinco anos em dois sistemas de informação no estado de São Paulo. A amostra compreendeu 2.117.108 crianças do Sistema de Vigilância Alimentar e Nutricional (Sisvan) e 748.551 do Projeto Estadual do Leite (Vivaleite). Inicialmente, avaliamos a frequência de valores faltantes e fora do espectro do equipamento, e calculamos o índice de preferência de dígito para peso. Após calcular os índices de altura para idade (A-I), peso para idade (P-I) e índice de massa corporal para idade (IMC-I), identificamos os valores biologicamente implausíveis (VBI) e calculamos o desvio-padrão (DP). Para cada município, calculamos a média e o DP de A-I, P-I e IMC-I; e plotamos os valores de DP em função da média. A preferência de dígito no peso foi maior em crianças de 24 a 59 meses no Sisvan. A frequência de VBI para A-I (SISVAN 2,56%; Vivaleite 0,98%) foi maior do que para P-I (Sisvan 2,10%; Vivaleite 0,18%). Para o índice A-I as variações entre os municípios foram mais acentuadas no Vivaleite do que no Sisvan. A variável altura apresentou baixa confiabilidade nos dois sistemas. A variável peso apresentou qualidade satisfatória no Vivaleite e insatisfatória no Sisvan. Paulo 2117108 2 117 108 2.117.10 Sisvan 748551 748 551 748.55 Vivaleite. . (Vivaleite) Inicialmente equipamento AI, AI I , (A-I) PI P (P-I IMCI, IMCI IMC (IMC-I) (VBI desviopadrão desvio padrão DP. (DP) município I, IMC-I 5 SISVAN 2,56% 256 56 0,98% 098 0 98 2,10% 210 10 0,18%. 018 0,18% 18 0,18%) 211710 11 2.117.1 74855 74 55 748.5 (Vivaleite (A-I (IMC-I (DP 2,56 25 0,98 09 9 2,10 21 1 01 0,18 21171 2.117. 7485 7 748. 2,5 0,9 2,1 0,1 2117 2.117 2, 0, 211 2.11 2.1 2.

Abstract Dairy production in Ecuador is a source of economic income for many small and medium producers, however the lack of technical advice leads to problems in the quality of milk, and affects their production volume. The purpose of this investigation was to conduct a pilot plan to improve the quality of milk produced by 57 small producers from different parishes of the cantons of Quito, Rumiñahui and San Miguel de los Bancos in the province of Pichincha, through a training plan in good milking practices and feeding strategies. Two initial samplings were carried out (between October and November 2021) prior to the training, and two final samplings (in June 2022) to measure the impact of the training. A total of 1.273 samples were collected, of these only 4.02 % (52/1273) (of the samples) complied with the reference values of the national regulations, while 95.98 % (1243/1273) did not comply with any of the evaluated parameters; however, the vast majority of the milk is suitable for human consumption, yet its nutritional quality needs to be improved. We were able to reduce considerably (although not statistically) the somatic cell count (SC, from an initial average of 623530 CS ml-1 to 365660 CS ml-1 after training, being correlated with the significant increase in the percentage of lactose between the initial and final sampling; there were also significant differences in the percentage of fat and total solids, although in these two cases their values decreased after training (fat from 2.58 % in the initial sampling to 2.10 % in the final sampling and total solids of 11, 44 % to 11.00 %);showed no significant differences between samples. It is concluded that the training was beneficial since the considerable decrease in the somatic cell count increased the volume of milk production of the small producers, and therefore their economic income. For the rest of the parameters, it is recommended to reinforce training in nutrition, feeding, pasture management, etc., in order to increase their values, as well as to maintain constant training programs for producers, supported by academia, private and public entities.

Resumen La producción láctea en Ecuador es una fuente de ingresos económicos para muchos pequeños y medianos productores; sin embargo, la falta de asesoría técnica conlleva a que tengan problemas en la calidad de la leche y se vea afectado su volumen de producción, por lo que la presente investigación tuvo como finalidad realizar un plan piloto para el mejoramiento de la calidad de leche producida por 57 pequeños productores de diferentes parroquias de los cantones Quito, Rumiñahui y San Miguel de los Bancos de la provincia de Pichincha, mediante un plan de capacitación en buenas prácticas de ordeño y estrategias de alimentación. Se realizaron 2 muestreos iniciales (entre octubre y noviembre de 2021), previo a la capacitación, y 2 muestreos finales (en junio de 2022) para medir el impacto de estas. Se recolectaron 1.273 muestras, donde solamente el 4,02 % (52/1.273) de las mismas, cumplen con los valores referenciales de la normativa nacional, mientras que el 95,98 % (1.243/1.273) no cumple con alguno de los parámetros evaluados; sin embargo, la gran mayoría de dicha leche sí es apta para el consumo humano, pero requiere ser mejorada en su calidad nutricional. Se logró disminuir considerablemente (aunque no estadísticamente) el conteo de células somáticas, de un promedio inicial de 623.530 CS ml-1 a 365.660 CS ml-1 luego de la capacitación, siendo correlacionado con el aumento significativo del porcentaje de lactosa entre el muestreo inicial y el final; también existió diferencias significativas en el porcentaje de grasa y sólidos totales, aunque en estos dos casos disminuyeron sus valores luego de la capacitación (grasa de 2,58 % en el muestreo inicial a 2,10 % en el final y sólidos totales de 11,44 % a 11,00 %). El resto de los parámetros no mostraron diferencias significativas entre los muestreos. Se concluye que la capacitación fue beneficiosa, pues la disminución considerable del conteo de células somáticas aumentó el volumen de producción de leche de los pequeños productores y, por ende, sus ingresos económicos. Para el resto de los parámetros se recomienda reforzar las capacitaciones de nutrición, alimentación, manejo de pasturas, etc., para incrementar sus valores, así como mantener constantes programas de capacitación a los productores, apoyados desde la academia, la empresa privada y entidades públicas.

RESUMEN Introducción: la lactancia materna exclusiva es fundamental para el desarrollo del bebé en los primeros meses de vida. Sin embargo, en los últimos años, se ha observado una preocupante disminución en esta práctica. Objetivo: describir el comportamiento de la lactancia materna exclusiva y su relación con la edad, ocupación y nivel de conocimiento de las madres. Métodos: estudio descriptivo observacional, transversal, población de 174 madres, de las cuales se seleccionó una muestra de 148, mediante muestreo probabilístico por cuotas, eligiéndose participantes aleatoriamente bajo criterios de inclusión y exclusión, en el último trimestre del 2022 en dos áreas de salud del municipio Holguín. Resultados: el 47 % de las madres dejaron de amamantar su hijo de forma exclusiva en los tres primeros meses de edad, el 26 % a los cinco meses y el 27 % lo mantienen por un tiempo mayor a seis meses, el grupo más representativo en el estudio son las madres entre los 20 y 30 años edad, predominan las que trabajan y predominan en un 90,5 % el conocimiento sobre el tema de lactancia materna exclusiva, no demostrándose relación significativa entre estas variables. La hipogalactia fue la razón principal del abandono precoz de la lactancia materna exclusiva. Conclusiones: la duración de la lactancia materna exclusiva se encuentra por debajo de las recomendaciones de la OMS, el abandono de esta práctica está asociado a no tener suficiente leche, a la incorporación al trabajo o escuela y que el niño no aumenta de peso.

ABSTRACT Introduction: exclusive breastfeeding is essential for the development of the baby in the first months of life. However, in recent years, a worrying decrease in this practice has been observed. Objective: to describe the behavior of exclusive breastfeeding and its relationship with mothers' age, occupation and level of knowledge. Methods: observational, cross-sectional, descriptive study, population of 174 mothers, from which a sample of 148 was selected, by means of probabilistic sampling by quotas, choosing participants randomly under inclusion and exclusion criteria, in the last quarter of 2022 in two health areas of Holguin municipality. Results: 47 % of the mothers stopped breastfeeding their child exclusively in the first three months of age, 26 % at five months and 27 % maintain it for more than six months, the most representative group in the study are mothers between 20 and 30 years of age, predominantly those who work and 90,5 % of them have knowledge on the subject of exclusive breastfeeding, not showing a significant relationship between these variables. Hypogalactia was the main reason for early abandonment of exclusive breastfeeding. Conclusions: the duration of exclusive breastfeeding is below WHO recommendations, the abandonment of this practice is associated to not having enough milk, to the incorporation to work or school and that the child is not able to breastfeed exclusively.

Resumen En un espacio limitado y con una amplia oferta de productos, el ruido visual de los empaques es abrumador. En este estudio se propone que un empaque con un diseño simple impacta positivamente al consumidor, primero, porque se percibe como un empaque prémium y, segundo, porque aumenta la intención de compra. En la metodología se usó un diseño experimental intrasujeto. Los participantes fueron expuestos aleatoriamente a dos imágenes de producto, en este caso, leche líquida: un diseño de empaque simple y otro complejo. Luego respondieron una encuesta en la que expresaron su percepción prémium del producto y su intención de compra. Los resultados soportaron la mediación total: el empaque simple, en comparación con el complejo, aumentó la percepción de que se está ante un producto prémium, lo que a su vez aumentó la intención de compra. Clasificación JEL: M30; M31; M39.

Abstract In a limited shelf space and with a wide product offering, the visual noise of packaging can be overwhelming. This study proposes that packaging with a simple design positively impacts the consumer: firstly, it is perceived as premium packaging, and secondly, it increases purchase intention. The methodology uses an intra-subject experimental design. Participants were randomly exposed to two product images, in this case, liquid milk: one with a simple packaging design and the other with a complex design. Next, they completed a survey where they indicated their perception of the product as premium and their purchase intention. The results support full mediation: compared to complex packaging, simple packaging increased the perception that the product is premium, which in turn increases purchase intention.

Resumo Em um espaço limitado e com uma ampla oferta de produtos, o ruído visual das embalagens é esmagador. Este estudo propõe que uma embalagem com um desenho simples impacta positivamente o consumidor, primeiro, porque é percebida como uma embalagem premium e, segundo, porque aumenta a intenção de compra. Na metodologia, foi utilizado um desenho experimental intra-sujeito. Os participantes foram expostos aleatoriamente a duas imagens de produto, neste caso, leite líquido: um desenho de embalagem simples e outro complexo. Em seguida, responderam a uma pesquisa na qual expressaram sua percepção premium do produto e sua intenção de compra. Os resultados suportaram a mediação total: a embalagem simples, em comparação com a complexa, aumentou a percepção de que se trata de um produto premium, o que, por sua vez, aumentou a intenção de compra.

Resumo A região do Vale do Taquari (VT) apresenta uma condição semelhante ao Rio Grande do Sul (RS) em relação ao desenvolvimento econômico alcançado, sendo este considerado um Estado com um “bom padrão” quando comparado às demais regiões do país. Apesar de padrões semelhantes, a trajetória seguida pela região por vezes acompanhou a do Estado, em outros momentos foi mais acelerada e, ainda, em alguns não seguiu a tendência. Historicamente, a produção primária tem uma importância significativa para a economia regional e geralmente está organizada em sistema integrado com a indústria de alimentos. Neste sentido, o trabalho analisa a evolução histórica da produção pecuária que sustenta a matriz produtiva no Vale do Taquari, RS, desenvolvida em especial pela agricultura familiar, e os desafios ambientais decorrentes da atividade. A pesquisa caracteriza-se como quantitativa e foi realizada com base em dados secundários, provenientes dos Censos Agropecuários divulgados pelo Instituto Brasileiro de Geografia e Estatística (IBGE). Os resultados da pesquisa apresentam que, muito embora tenha ocorrido um aumento importante na atividade pecuária, a dinâmica intrarregional e a evolução por espécie produtiva ocorreram de forma diferente: se, por um lado, no caso dos bovinos, a evolução foi equilibrada entre os municípios, por outro lado, no caso das aves, suínos e da produção leiteira, alguns municípios reduziram sua participação, enquanto em outros houve um incremento significativo da atividade. Contudo, a intensificação das atividades em determinados municípios, também têm imposto desafios ambientais. VT (VT RS (RS alcançado bom padrão país semelhantes ainda tendência Historicamente alimentos sentido familiar caracterizase caracteriza se secundários IBGE. IBGE . (IBGE) diferente lado bovinos aves leiteira participação Contudo (IBGE

Abstract The region of Taquari Valley (VT) shows a similar condition regarding to Rio Grande do Sul (RS) in relation to the achieved economic development, which is considered a “good standard” state compared to other regions of the country. Despite the similar patterns, the trajectory followed by the region under study sometimes followed the State’s trajectory, in other moments it was faster and even, sometimes it did not follow the trend. Historically, primary production has a meaningful importance in the regional economy and is usually organized in an integrated system with the food industry. This work analyzes the historical evolution of livestock production that sustains the productive matrix in Vale do Taquari, RS, developed especially by family farming, and the environmental challenges arising from the activity. The research is characterized as quantitative, and it was based on secondary data from the Agricultural Census released by the Brazilian Institute of Geography and Statistics (IBGE). The results of the research show that although there has been a significant increase in the cattle activity, the intra-regional dynamics and the evolution by productive species occurred differently: in the case of cattle and milk production the evolution was balanced among cities, on the other hand, in the case of poultry and swine, some cities reduced their participation, whereas in others there was a significant increase in the activity. However, the intensification of activities in certain municipalities has also imposed environmental challenges. VT (VT RS (RS development good standard country patterns States State s even trend Historically industry farming activity quantitative IBGE. IBGE . (IBGE) intraregional intra differently hand swine participation However (IBGE

Resumen La región de Vale do Taquari (VT) presenta una condición similar a Rio Grande do Sul (RS) en relación al desarrollo económico alcanzado, que es considerado un estado de “buen estándar” en comparación con otras regiones del país. A pesar de patrones similares, la trayectoria seguida por la región a veces siguió la del Estado, otras veces fue más acelerada y, aun así, en algunos casos no siguió la tendencia. Históricamente, la producción primaria ha tenido una importancia significativa para la economía regional y generalmente se organiza en un sistema integrado con la industria alimentaria. En ese sentido, el trabajo analiza la evolución histórica de la producción pecuaria que sostiene la matriz productiva en Vale do Taquari, RS, desarrollada especialmente por la agricultura familiar, y los desafíos ambientales derivados de la actividad. La investigación se caracteriza por ser cuantitativa y se realizó a partir de datos secundarios de los Censos Agropecuarios publicados por el Instituto Brasileño de Geografía y Estadística (IBGE). Los resultados de la investigación muestran que, si bien ha habido un aumento significativo de la actividad ganadera, la dinámica intrarregional y la evolución por especies productivas se dieron de manera diferente: si, por un lado, en el caso del ganado vacuno, la evolución fue equilibrada entre el los municipios, por otro lado, en el caso de la producción avícola, porcina y láctea, algunos municipios redujeron su participación, mientras que en otros hubo un incremento significativo en la actividad. Sin embargo, la intensificación de actividades en ciertos municipios también ha impuesto desafíos ambientales. VT (VT RS (RS alcanzado buen estándar país similares Estado así tendencia Históricamente alimentaria sentido familiar IBGE. IBGE . (IBGE) ganadera diferente lado vacuno avícola láctea participación embargo (IBGE

Abstract Introduction and Objectives: Oral food challenges (OFC) are the gold standard for the diagnosis of food allergy. The aim of this study was to characterize and analyze the OFCs performed in the Pediatric Department of the study hospital. Material and Methods: Retrospective analysis of all patients who underwent OFCs in the pediatric department of the study hospital from October 2016 to December 2021. All non-IgE-mediated reactions were excluded. Statistical analysis was performed using IBM Statistical Package for Social Sciences®. Results: A total of 301 OFCs were performed on a total of 172 patients during the study period. Most patients were preschool children (<6 years; 79.4%) and 40.1% had more than one OFC. The most frequently tested allergens were hen’s egg (26.2%), tree nuts (16.9%), and cow’s milk (16.7%). The positivity rate was 23.2%. Tests for milk and wheat were significantly associated with a positive result. The main reason for OFC testing was to establish a diagnosis (60.1%), but also to assess tolerance (25.6%) and to document the responsiveness threshold (14.3%). Most positive reactions were local/grade I (25.7%) or moderate/grade II (58.6%). Patients with a personal history of atopic comorbidities were more likely to have a positive test result (p=0.022), especially those with atopic dermatitis (p=0.022). Discussion and Conclusion: Most OFCs in this analysis were negative. Early recognition of food allergy resolution is essential to prevent unnecessary allergen avoidance. An increased risk of positive reactions to milk or wheat, or in the setting of a personal history of atopic dermatitis, should be considered.

Resumo Introdução e Objetivos: A prova de provocação oral é o gold standard para o diagnóstico de alergia alimentar. Este estudo pretendeu caracterizar e analisar as provas efetuadas no Serviço de Pediatria de um hospital de nível II. Material e Métodos: Estudo retrospetivo dos doentes submetidos a provas de provocação oral entre outubro 2016 e dezembro 2021 num hospital de nível II. As reações não mediadas por IgE foram excluídas. A análise estatística foi realizada através do software IBM Statistical Package for Social Sciences®. Resultados: Foram realizadas 301 provas num total de 172 doentes. A maioria dos doentes eram crianças com idade <6 anos (79,4%) e 40,1% realizou duas ou mais provas. Os alergénios mais frequentemente testados foram ovo (26,2%), frutos secos (16,9%) e leite de vaca (16,7%). Constatou-se que 23,2% das provas foram positivas. Verificou-se uma associação estatisticamente significativa entre as provas realizadas para o leite e trigo e a obtenção de um resultado positivo. Relativamente ao objetivo das provas, 60,1% destinavam-se a estabelecer o diagnóstico, 25,6% a avaliar a tolerância e 14,3% a documentar o limiar de reatividade. A maioria das reações positivas foram locais/grau I (25,7%) ou moderadas/grau II (58,6%). Foi identificada uma associação estatisticamente significativa entre antecedentes de atopia e um resultado positivo nas provas (p=0,022), particularmente antecedentes de dermatite atópica (p=0,022). Discussão: A maioria das provas realizadas foram negativas. O reconhecimento atempado da resolução de uma alergia alimentar permite suspender a evicção desnecessária de alergénios suspeitos. Deve ser considerado o risco acrescido de uma reação positiva com leite, trigo ou em contexto de antecedentes pessoais de dermatite atópica.