Resumo Vaginite aeróbica (VA) é uma infecção vaginal recorrente definida recentemente, que é tratada com antibióticos. No entanto, o uso excessivo e prolongado de antibióticos perturba a microflora vaginal saudável e leva ao surgimento de resistência aos antibióticos entre os patógenos. Esta situação levou os pesquisadores a explorar antimicrobianos alternativos. O presente estudo descreve a eficácia antimicrobiana in vitro e in vivo e as interações farmacêuticas entre óleos essenciais vegetais (OE) e cinco bactérias lácticas (BAL), isoladas de vagina sã, contra E. faecalis, um dos principais agentes etiológicos da AV. Os experimentos in vitro confirmam a boa atividade antimicrobiana de ambos os EOs de plantas e sobrenadante livre de células (CFS) de LABs. Com base na alta eficácia antimicrobiana, o óleo essencial de Moringa (MO) foi selecionado para determinar sua natureza de interação com o sobrenadante livre de células (CFS) de cinco cepas de LAB. Sinergismo foi registrado entre MO e CFS de L. reuteri (MT180537). Para validar os resultados in vitro, as respostas profiláticas da aplicação individual e sinérgica de MO e L. reuteri (MT180537) foram avaliadas em um modelo murino AV induzido por E. faecalis (MW051601). A eficácia profilática foi evidenciada por uma redução na intensidade dos sintomas clínicos, contagem de E. faecalis (MW051601) por tecido vaginal, juntamente com uma redução nas alterações associadas a AV nos marcadores histológicos de infecção em animais que receberam óleo essencial de Moringa e L. reuteri (MT180537) sozinho ou em combinação. No entanto, não foi possível observar sinergismo significativo entre o óleo essencial de Moringa e L. reuteri (MT180537). Nossos dados confirmam a importância dos experimentos in vivo na dedução de interações farmacológicas.

Abstract Aerobic vaginitis (AV) is a recently defined vaginal recurring infection, which is treated with antibiotics. However, excessive and prolonged use of antibiotics disrupts healthy vaginal microflora and leads to the emergence of antibiotic resistance among pathogens. This situation has directed researchers to explore alternative antimicrobials. The current study describes in vitro and in vivo antimicrobial efficacy and pharmaceutical interactions between plant essential oils (EOs) and five lactic acid bacteria (LABs), isolated from the healthy vagina, against E. faecalis, one of the major etiological agents of AV. In vitro experiments confirm good antimicrobial activity of both plant EOs and cell free supernatant (CFS) from LABs. Based on high antimicrobial efficacy, Moringa essential oil (MO) was selected to determine its nature of interaction with CFS of five LAB strains. Synergism was recorded between MO and CFS of L. reuteri (MT180537). To validate in vitro findings, prophylactic responses of individual and synergistic application of MO and L. reuteri (MT180537) were evaluated in an E. faecalis (MW051601) induced AV murine model. The prophylactic efficacy was evidenced by a reduction in intensity of clinical symptoms, E. faecalis (MW051601) count per vaginal tissue along with a reduction in AV associated changes in histological markers of infection in animals receiving Moringa essential oil and L. reuteri (MT180537) alone or in combination. However, significant synergism between Moringa essential oil and L. reuteri (MT180537) could not be observed. Our data confirms the importance of in vivo experiments in deducing pharmacological interactions.

Resumo O estudo teve como objetivo analisar os efeitos dos antibióticos promotores de crescimento (AGPs), di-hidrato de oxitetraciclina e fosfato de tilosina na microflora intestinal de frangos de corte. Os AGPs foram fornecidos em diferentes concentrações isoladamente ou em combinações por 42 dias de criação. Amostras fecais foram coletadas do intestino (duodeno, jejuno e ceco) de frangos de corte no 14º, 28º e 42º dias de ensaio. As amostras foram cultivadas em diferentes meios seletivos e a identificação bacteriana foi realizada por diferentes ferramentas de diagnóstico bioquímico e molecular. Os resultados mostraram um efeito significativo dos AGPs no crescimento de microrganismos patogênicos como Escherichia coli e Clostridium perfringens no intestino. Curiosamente, um crescimento prejudicado foi observado para ambas as bactérias, mostrando um efeito significativo (P <0,05) de AGPs em E. coli e C. perfringens nos dias 14, 28 e 42. Este efeito foi observado apenas e em combinação com o uso de AGPs. Os dados mostraram ainda que o efeito foi mais proeminente em combinação e com um aumento da concentração de AGPs. Nenhum comprometimento foi observado no crescimento de L. reuteri em diferentes locais do intestino e duração (14º, 28º e 42º dias). Os resultados mostraram que o uso de AGPs na dieta não tem efeito nocivo nas bactérias benéficas, no entanto, foi observado um crescimento prejudicado nas bactérias nocivas. Sugere-se que uma combinação de AGPs (OXY-1.0+TP-0.5) seja econômica e não tenha efeito prejudicial sobre o frango de corte. O uso de AGPs em uma dose recomendada e por um período de tempo específico é seguro para uso em aves tanto como promotor de crescimento quanto para prevenção de doenças.

Abstract The study was aimed to analyse the effects of antibiotic growth promoters (AGPs), Oxytetracycline di-hydrate and Tylosin phosphate on the intestinal microflora in broiler chicken. The AGPs were provided in different concentrations solely or in combinations for 42 days of rearing. Faecal samples were collected from the intestine (duodenum, jejunum and caeca) of broiler chicken on 14th, 28th and 42nd days of trial. Samples were cultured on different selective medium and bacterial identification was performed by different biochemical and molecular diagnostic tools. Results showed a significant effect of AGPs on the growth of pathogenic microorganisms such as Escherichia coli and Clostridium perfringens in the intestine. Interestingly, an impaired growth was observed for both bacterium showing a significant effect (P<0.05) of AGPs on E. coli and C. perfringens on day 14th, 28th, and 42nd. This effect was observed solely and in combination while using AGPs. Data further showed that the effect was more prominent in combination and with an increase concentration of AGPs. Remarkably, no impairment was seen on the growth of L. reuteri at different sites of intestine and duration (14th, 28th, and 42nd days). The results showed that the use of AGPs in diet has no harmful effect on beneficial bacteria, however, an impaired growth was seen on the harmful bacteria. It is suggested that a combination of AGPs (OXY-1.0+TP-0.5) is economical and have no harmful effect on the broiler chicken. The use of AGPs in a recommended dose and for a specific period of time are safe to use in poultry both as growth promoter and for the prevention of diseases.

Resumo A microbiota gastrointestinal regula as funções do corpo e desempenha um papel importante na sua saúde. A disbiose leva a uma série de doenças crônicas, como diabetes, obesidade, inflamação, aterosclerose, etc. No entanto, essas doenças podem ser prevenidas pelo uso de probióticos − microrganismos vivos que beneficiam a microflora e, portanto, melhoram a saúde do organismo hospedeiro. Os probióticos mais comuns incluem bactérias do ácido láctico dos gêneros Bifidobacterium e Propionibacterium. Nós estudamos as propriedades probióticas das seguintes cepas: Bifidobacterium adolescentis АС-1909, Bifidobacterium longum infantis АС-1912, Propionibacterium jensenii В-6085, Propionibacterium freudenreichii В-11921, Propionibacterium thoenii В-6082 В-6082 acid e Propionibacterium thoenii В-6082 В-6082 acidibion. A atividade antimicrobiana foi determinada pelo método de 'blocos de ágar' contra as seguintes culturas de teste: Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa B6643, Proteus vulgaris ATCC 63 e Listeria monocytogenes moderada atividade ATCC 7644. Uma atividade antimicrobiana moderada contra todas as culturas de teste foi registrado em Bifidobacterium adolescentis АС-1909, Propionibacterium jensenii В-6085 e Propionibacterium thoenii В-6082. A atividade antioxidante foi determinada pelo método de inibição do DPPH em todas as cepas de ácido lático. Nosso estudo indicou que algumas cepas de Propionibacterium e Bifidobacterium − ou, teoricamente, seus consórcios − poderiam ser usadas como culturas probióticas em suplementos dietéticos ou alimentos funcionais para prevenir uma série de doenças crônicas.

Abstract The gastrointestinal microflora regulates the body’s functions and plays an important role in its health. Dysbiosis leads to a number of chronic diseases such as diabetes, obesity, inflammation, atherosclerosis, etc. However, these diseases can be prevented by using probiotics – living microorganisms that benefit the microflora and, therefore, improve the host organism's health. The most common probiotics include lactic acid bacteria of the Bifidobacterium and Propionibacterium genera. We studied the probiotic properties of the following strains: Bifidobacterium adolescentis АС-1909, Bifidobacterium longum infantis АС-1912, Propionibacterium jensenii В-6085, Propionibacterium freudenreichii В-11921, Propionibacterium thoenii В-6082, and Propionibacterium acidipropionici В-5723. Antimicrobial activity was determined by the ‘agar blocks’ method against the following test cultures: Escherichia coli ATCC 25922, Salmonella enterica ATCC 14028, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa B6643, Proteus vulgaris ATCC 63, and Listeria monocytogenes ATCC 7644. Moderate antimicrobial activity against all the test cultures was registered in Bifidobacterium adolescentis АС-1909, Propionibacterium jensenii В-6085, and Propionibacterium thoenii В-6082. Antioxidant activity was determined by the DPPH inhibition method in all the lactic acid strains. Our study indicated that some Propionibacterium and Bifidobacterium strains or, theoretically, their consortia could be used as probiotic cultures in dietary supplements or functional foods to prevent a number of chronic diseases.

The widespread use of antibiotics in poultry breeding has led to drug resistance of pathogenic microorganisms. With long-term dependence on antibiotics, immunity in poultry has decreased, increased susceptibility and seriously affecting the quality of poultry protein products and consumer health. Chinese medicinal herbs come mainly from nature and are natural products, so are called green feed additives. It has become an important substitute for antibiotics in high-quality livestock products. In this study, Wanxi White Geese were used as the research object to explore the effects of Chinese medicinal herb feed additives on intestinal microorganisms. Semen Cassiae and Polygonatum cyrtonema Hua increased the number of operational taxonomic units (OTUs) in intestinal microorganisms of geese, and the number of OTUs increased with dosage. Semen Cassiae or Polygonatum cyrtonema Hua has great influence on the abundance of intestinal microbes. The relative abundance of Clostridiales was higher than the control (feeding only the basal diet) after treated with Semen Cassiae or Polygonatum cyrtonema Hua feed additives. Chinese medicinal herb feed additives inhibited Erysipelotrichales and the relative abundance of Erysipelotrichales in all treated samples was lower than the control group. Alpha diversity analysis also showed that Chinese medicinal herb feed additives promoted the diversity of intestinal microorganisms. The purpose of this study is for future research and practical application of feed additives.

ABSTRACT This study investigates the dynamics of spoilage microflora in duck meat packed in vacuum and air during 9 days of storage. In total, 72 duck carcasses were used in the study. Samples of meat (breasts and thighs, with or without skin) were analysed on days 1, 3, 6 and 9 after packaging in two different atmospheres, at a storage temperature of 3°C ± 1°C. Enterobacteriaceae, coliform bacteria, and E. coli, Total Viable Count, psychrotrophic microorganisms, Lactic Acid Bacteria, Pseudomonas spp. counts, and Brochothrix thermosphacta were determined. As expected, the type of sample and storage length had the greatest influence on microbiological quality (p<0.001). Lower significance (p<0.01) was observed for the place of sampling factor - i.e. duck breast or thigh. As expected, better microbiological parameters were achieved with vacuum-packed samples. The pH value increased during storage, but the increase was minimal for the vacuum-packed samples. In all the microbiological parameters monitored, the best results at the end of storage were obtained for duck breast muscles packed in vacuum. total 7 breasts thighs skin 1 3 atmospheres 3C C 1C 1°C Enterobacteriaceae bacteria E coli Count microorganisms Bacteria spp counts determined expected p<0.001. p0001 p p<0.001 . 0 001 (p<0.001) p<0.01 p001 01 (p<0.01 ie i e i.e thigh vacuumpacked samples monitored p000 p<0.00 00 (p<0.001 p<0.0 p00 (p<0.0 (p<0.00 p<0. p0 (p<0. p<0 (p<0 p< (p< (p

Resumen La hesperidina es un flavonoide derivado de los cítricos con usos potenciales en la alimentación animal. Se investigaron los efectos de la suplementación con hesperidina en dietas para codorniz japonesa (Codornix codornix japonica) sobre la química sanguínea, los niveles de enzimas antioxidantes en ciertos tejidos, la histomorfología intestinal y la microflora fecal. Se llevó a cabo un ensayo de alimentación durante 35 días utilizando 300 codornices. Se dividieron en tres tratamientos según la dieta administrada: Control (0 g de hesperidina/kg de alimento); HES1 (1 g de hesperidina/kg de alimento); y HES2 (2 g de hesperidina/kg de alimento). En cada tratamiento se llevaron a cabo cinco réplicas de veinte animales cada una. Al final del periodo de estudio, se tomaron muestras de heces, sangre, hígado y tejido muscular del muslo. En sangre, la alanina transaminasa (ALT) y la lactato deshidrogenasa (LDH) fueron más bajas en HES1 que en el Control, pero aumentaron en HES2 (P<0.05). La aspartato transaminasa (AST) en la sangre aumentó en HES1, pero disminuyó en HES2 (P<0.05). La amilasa aumentó tanto en HES1 como en HES2 (P<0.05). Las enzimas antioxidantes GSH, CAT y SOD en los tejidos aumentaron (P<0.05). En el ciego y el colon, los tratamientos con hesperidina produjeron vellosidades más altas y criptas menos profundas que en el Control (P<0.05). La presencia de la hesperidina en la dieta para codornices no afectó la microflora en las heces. La adición de la hesperidina mostró efectos positivos en los niveles de enzimas antioxidantes en lípidos, muslos, hígado y suero, sobre la histomorfología intestinal y en la microflora fecal.

Abstract This study was conducted to determine the effects of hesperidin, a flavonoid added to quail diets, on blood serum, enzymes in tissues, intestinal histomorphology and fecal microflora. In the study, first treatment [(control) (0g hesperidin/kg feed)], second treatment [(HES1) (1 g hesperidin/kg feed)], third treatment [(HES2) (2 g hesperidin/kg feed)] was added to with the basal diet through 35 d. The study was carried out with 3 main groups, 20 quails with 5 sub-repeats in each group and a total of 300 quails. At the end of the study, blood, liver and thigh muscle tissue and fecal samples were taken. Alanine transaminase (ALT) and lactate dehydrogenase (LDH) decreased in the HES1 group but increased in the HES2 group compared to the control group (P<0.05). Aspartate transaminase (AST) increased in the HES1 group compared to the control group and decreased in the HES2 group (P<0.05). Amylase, on the other hand, showed a regular increase in HES1 and HES2 groups to which hesperidin was added to the control group in hesperidin added groups (P<0.05). The tissue antioxidant GSH, CAT and SOD enzyme parameters showed a significant increase in the hesperidin added groups compared to the control group, and this increase was found to be significant compared to the control group (P<0.05). In intestinal histomorphology, in hesperidin treatment groups increased the height of villus in the cecum tissue; in colon tissue, it was determined that hesperidin added groups increased villus height but decreased crypt depth (P<0.05). Consequently, diets hesperidin with treatments positively is thought to affect the lipid, thigh, liver and serum antioxidant enzyme levels, intestinal histomorphology and fecal microflora in quail.

Abstract This study explored effects of microbial preparation (MIP, developed by our research team) on production performance and body health of Boer goat to reveal the function of it in goat breeding industry. Compound yeast and enzyme preparation (CYP) was used to compare the function of MIP. Healthy male Boer goats (n = 15, BW = 25.31±4.06 kg) were allocated randomly into three groups as NC (Basal diet), MI (Basal diet + MIP) and CY (Basal diet + CYP). This study lasted for 71 days including 15 days for adaptation and 56 days for growth trial. Both MIP and CYP enhanced production performance such as average daily gain of goats, MIP enhanced the level of fat synthesis such as total cholesterol level significantly on day 28. As for rumen fermentation and microbial communities of goat, MIP decreased rumen pH. PCoA analysis showed that the rumen bacterial community on day 28 was significantly separated. In conclusion, MIP increased production performance, ameliorating rumen fermentation and shifting microflora. Our findings provide the evidence for the influence of probiotics on goat production performance as well as health condition. MIP, (MIP team industry (CYP n 2531406 25 31 4 06 25.31±4.0 kg Basal diet, , diet) CYP. . CYP) 7 1 5 trial pH 2 separated conclusion microflora condition 253140 3 0 25.31±4. 25314 25.31±4 2531 25.31± 253 25.31 25.3 25.

ABSTRACT This experiment was conducted to investigate the effects of lactic acid bacteria preparations on microbial diversity and community structure of calves. On days 1 and 7 of the trial period, feces were collected into sterile tubes and labeled (Day 1: control group D1DZ, experimental group D1SY, and Day 7: control group D7DZ, experimental group D7SY). Twenty Angus calves (150±10 kg) were selected and randomly divided into two groups of 10 calves each. The control group fed a basal diet. In addition to feeding the basal diet, the experimental group was given 15 mL lactobacillus preparation orally at 09:00 and 16:00 h every day. Calves were allowed free feeding and drinking water. All other feeding environments and management conditions remained consistent with the experiment lasting for seven days. At the end of the experiment, the fecal microflora of the calves was analyzed using 16S rRNA sequencing techniques. The 16S rRNA analysis data were processed using the Excel 2007 software and analyzed by the IBM SPSS statistical software (Statistical Analysis System, version 22). The Alpha diversity index analysis showed that the Chao and the Ace indices were significantly different after feeding supplemented with lactic acid bacteria. The PCA analysis showed that the fecal flora structure differed significantly after supplementation with the lactic acid bacteria preparation. Further analysis showed that the lactic acid bacteria increased Firmicutes, Patescibacteria, Rikenellaceae_RC9_gut_group, Clostridium_sensu_stricto_1, and prevotellaceae_UCG-003 in the feces. Therefore, we speculate that lactic acid bacteria preparations play an important role in animal production and are beneficial to the diversity of the fecal microflora of the calves. period D1DZ DDZ D DZ D1SY DSY SY D7DZ D7SY. D7SY . D7SY) 150±10 15010 150 (150±1 kg each diet 0900 09 00 09:0 1600 16 16:0 day water S techniques 200 Statistical System 22. 22 22) Firmicutes Patescibacteria RikenellaceaeRC9gutgroup RikenellaceaeRCgutgroup Rikenellaceae RC9 gut RC Rikenellaceae_RC9_gut_group Clostridiumsensustricto1 Clostridiumsensustricto Clostridium sensu stricto Clostridium_sensu_stricto_1 prevotellaceae_UCG003 prevotellaceaeUCG003 prevotellaceaeUCG prevotellaceae_UCG 003 prevotellaceae UCG prevotellaceae_UCG-00 Therefore 150±1 1501 (150± 090 0 09: 160 16: 20 2 RikenellaceaeRC gutgroup Clostridium_sensu_stricto_ UCG003 prevotellaceae_UCG00 prevotellaceaeUCG00 prevotellaceae_UCG-0 150± (150 Clostridium_sensu_stricto UCG00 prevotellaceae_UCG0 prevotellaceaeUCG0 prevotellaceae_UCG- (15 UCG0 (1 (

Abstract This study investigated the effect of Lactobacillus isolated from Chinese fermented food on antibiotic-induced intestinal microflora disorder during the early life of mice. The experimental strain Lactobacillus fermentum (LF) CQPC04 was isolated from naturally fermented pickles in Chongqing, China. Lactobacillus plantarum (LP) KFY02 was isolated from naturally fermented yogurt from Korla in the Xinjiang Uygur Autonomous Region. The results showed that LF-CQPC04 and LP-KFY02 alleviated the decrease in bacterial diversity caused by the antibiotics, maintained the abundance of beneficial bacteria, and reduced the abundance of harmful bacteria. These results suggest that LF-CQPC04 and LP-KFY02 can be used as probiotics to alleviate antibiotic-induced intestinal microfloral disorder. antibioticinduced antibiotic induced mice LF (LF CQPC CQPC0 Chongqing China LP (LP KFY KFY0 Region LFCQPC04 LFCQPC LF-CQPC0 LPKFY02 LPKFY LP-KFY0 antibiotics bacteria LFCQPC0 LF-CQPC LPKFY0 LP-KFY

This study investigated the effect of far-infrared ray (FIR) illumination on performance, blood biochemistry, and faecal microflora of laying hens at different production stages. A total of 360 Hy-line brown laying hens were randomly allocated in a 2 χ 2 factorial arrangement with six replicates. Hens were distributed in two production stages (30-39 and 45-54 weeks-old). Each group was exposed to two light types (light emitting diode; LED and LED+FIR) in separate rooms. The LED treatment illuminated a wavelength of 650 ± 10 nm (0.65 ± 0.01 μηι), while LED+FIR treatment emitted 15 ± 10 μπι with an LED wavelength. The results showed an interaction between egg production stages and light types on the serum triglyceride concentration. The hens exposed to the LED+FIR and LED treatments showed similar egg production, feed intake, egg weight, feed conversion ratio (FCR), as well as albumen height, haugh unit, and shell thickness of eggs. LED+FIR substantially decreased the concentration of serum cholesterol (CHOL), HDL cholesterol (HDLC), and triglyceride (TG) compared to LED lighting. LED+FIR substantially reduced the number of total microbes, Escherichia coli, and Salmonella in faeces compared to birds exposed to LED light. These findings suggest that LED+FIR lights may improve hen health and the hen house environment.

Abstract In scientific and economic experience, the effect of zeolite-chlorella top dressing (CCP) from 28-29% zeolite, 1.5-2% Chlorella vulgaris powder and 75-76% cake on the productive effect of feed and digestion metabolism of dairy cows has been studied. Tetra-edric frame-hollow zeolite crystals have selectively adsorbing and ion-filtering properties, and chlorella enriches with amino acids and vitamins. The introduction of CCP into the diet of cows of the experimental group increased the mineral-vitamin balance and positively affected the metabolism of cicatricial digestion. The pH shift from 6.14 to 6.17 activated the ecosystem of the rumen microflora, increased the synthesis of LVH 0.79 mmol/100ml, due to an increase in the volume of acetates from 54.1±3.0 to 57.2±2.2 mmol/100ml, increased the number of infusoria by 41.1 thousand/ml more than the control group. An increase in the amylolytic activity of the rumen chyme by 2.8 mg/starch, and cellulolytic activity by 2.8% increased scar digestion. This increased the consumption of the dry matter of the diet by cows of the experimental group by 0.48 ± 0.06 kg/head. /day. and daily milk yields by 1.06 ± 0.03 kg against the control. An increase in protein and fat in milk with a decrease in somatic cells, and in the blood of erythrocytes and hemoglobin increased biosynthesis, which increased the conversion rate of feed from 0.68 to 0.72, and protein from 17.0% to 18.9%. experience zeolitechlorella (CCP 2829% 2829 28 29% 29 28-29 1.52% 152 1.5 2% 1 5 2 1.5-2 7576% 7576 75 76% 76 75-76 studied Tetraedric Tetra edric framehollow frame hollow ionfiltering ion filtering properties vitamins mineralvitamin mineral vitamin 614 6 14 6.1 617 17 microflora 079 0 79 0.7 mmol100ml mmolml mmol 100ml ml mmol/100ml 54130 54 3 54.1±3. 57222 57 57.2±2. 411 41 41. thousandml thousand 8 2. mgstarch mg starch mg/starch 048 48 0.4 006 06 0.0 kghead head kg/head day /day 106 1.0 003 03 cells biosynthesis 068 68 0.6 072 72 0.72 170 17.0 189 18 9 18.9% 282 28-2 52 1.52 15 1. 1.5- 757 7 75-7 61 6. 07 0. 5413 54.1±3 5722 57.2±2 4 04 00 10 17. 18.9 28- 75- 541 54.1± 572 57.2± 18. 54.1 57.2 54. 57.

Resumo A experiência científica e econômica estudou o efeito produtivo da alimentação e o metabolismo da digestão de vacas leiteiras, de Zeolite-Chlorella (CHP), de 28-29% de zeólita, 1,5-2% de pó de Chlorella vulgaris e 75-76% de bagaço. Os cristais ocos de estrutura tetraedrítica do zeólito possuem propriedades seletivas de adsorção e ionofiltração, e a Chlorella enriquece com aminoácidos e vitaminas. A introdução na dieta de vacas de um grupo experiente de CHP aumentou o equilíbrio mineral-vitamínico e afetou significativamente o metabolismo da digestão cicatricial. A mudança de pH de 6,14 para 6,17 ativou o ecossistema da microflora cicatricial, aumentou a síntese de LLA 0,79 mmol/ 100ml, devido à uveistivanie do volume de acetatos de 54,1±3,0 para 57,2±2,2 mmol/100ml, aumentou o número de infusórios em 41,1 mil/ml mais do que o grupo controle. O aumento da atividade amilolítica do quimo da cicatriz em 2,8 mg / amido e da atividade celulosolítica em 2,8% aumentou a digestão cicatricial. Isso aumentou a ingestão de matéria seca das vacas do grupo experimental em 0,48±0,06 kg/gol./dia e a produção diária de leite em 1,06±0,03 kg contra o controle. O aumento da proteína e da gordura no leite, com a diminuição das células somáticas, e no sangue de glóbulos vermelhos e hemoglobina aumentou a biossíntese, o que aumentou a taxa de conversão alimentar de 0,68 para 0,72 e proteína de 17,0% para 18,9%. leiteiras ZeoliteChlorella Zeolite CHP, , (CHP) 2829% 2829 28 29% 29 28-29 zeólita 1,52% 152 1,5 2% 1 5 2 1,5-2 7576% 7576 75 76% 76 75-76 bagaço ionofiltração vitaminas mineralvitamínico mineral vitamínico cicatricial 614 6 14 6,1 617 17 079 0 79 0,7 mmol 100ml ml 54130 54 3 54,1±3, 57222 57 57,2±2, mmol100ml mmolml mmol/100ml 411 41 41, milml mil controle 8 2, 048006 48 06 0,48±0,0 kggoldia gol dia 106003 03 1,06±0,0 somáticas biossíntese 068 68 0,6 072 72 170 17,0 189 18 9 18,9% (CHP 282 28-2 52 1,52 15 1, 1,5- 757 7 75-7 61 6, 07 0, 5413 54,1±3 5722 57,2±2 4 04800 0,48±0, 10600 1,06±0, 17, 18,9 28- 75- 541 54,1± 572 57,2± 0480 0,48±0 1060 1,06±0 18, 54,1 57,2 048 0,48± 106 1,06± 54, 57, 04 0,48 10 1,06 0,4 1,0

ABSTRACT Gut microbiota plays an important role in animal health, production and diseases. Little is known about whether heat stress alters the composition of quail gut microbiota; therefore, we studied the effects of heat stress on growth performance, antioxidant functions, heat shock proteins and caecal microbiota. Two groups of 40 (20-day-old) quails were set up, including a control reared at 24 ± 2°C and a heat stress group subjected to heat stress at 36 ± 2°C for 4 h per day for 7 consecutive days. We found that heat stress significantly elevated the relative expression levels of HSP70 and HSP90 mRNA in the thymus, bursa and spleen by real-time fluorescence quantitative PCR assay, indicating successful establishment of the experimental model. Heat stress was found to have an effect on gut microbiota composition. At the genus level, Alistipes were significantly increased in the heat stress group. PICRUSt2 function prediction revealed that most of the KEGG pathways with high temperature-induced abundance differences are metabolism-related. These data indicated that heat stress reduced the production performance of quails by affecting antioxidant functions, as well as the composition and structure of the intestinal microbiota. The results of this study provide technical information for conducting research on heat stress prevention and control techniques in quails. health diseases therefore functions 20dayold dayold 20 old (20-day-old up 2 2C C 3 days HSP HSP7 HSP9 thymus realtime real time assay model level PICRUSt temperatureinduced temperature induced metabolismrelated. metabolismrelated metabolism related. related metabolism-related

Abstract The aim of this study is to investigate the effect of salep species grown in different regions of Turkey on the rheological and microstructural properties of ice cream mix and the thermal and sensory properties of ice cream. For this purpose, ten different types of salep grown in different regions (Mersin, Yozgat, Muğla, Kahramanmaraş, Adana, Van, Muş) in the natural microflora of Turkey were used in a formulation of an ice cream mix. The flow behavior, frequency sweep, and 3-ITT rheological properties of ice cream mixes were studied. All samples showed shear thinning, viscoelastic solid-like, and recoverable character. The K and n values for the ice cream mixes were determined as 0.03-35.08 Pasn and 0.33-0.80, respectively, and significantly differed according to salep varieties (p < 0.05). The zeta potential values of ice cream mix samples ranged from -25.87 mV to -33.95 mV and were significantly affected by salep varieties (p < 0.05). The use of different salep varieties significantly affected the thermal properties of ice cream such as freezing point temperature (Tf), temperature range (ΔT), and enthalpy of fusion (ΔHf). In conclusion, the results of this study showed that the use of different salep varieties can significantly affect the rheological, thermal, and sensory properties of ice cream, and the selection of salep varieties may be vital for the desired quality of ice cream.

ABSTRACT Introduction: oral hygiene is fundamental to achieve good oral health and thus avoid multiple pathologies, among them dental caries, the most frequent. It is a disease of multifactorial origin in which there is an interaction of three main factors: the host (particularly the tooth and saliva), the microflora (microorganisms) and the substrate (diet). Objective: to determine the level of oral hygiene in a population of the "La Palmira" neighborhood, in the city of Salcedo, Ecuador. Methods: observational, descriptive, cross-sectional, descriptive research carried out in the period May - October 2023. The universe was composed of 1200 people, the sample obtained through simple random sampling, according to the inclusion and exclusion criteria, was composed of 291 people. Results: of the population studied, 34 % indicated that they brushed their teeth twice a day (70,5 %) and 62,9 % indicated that they brushed three times a day or more. As for the use of alternative means, in this case mouthwash, 39,9 % indicate that they occasionally use mouthwash, compared with the validated survey, this is 15 %, 22 % indicate that they use mouthwash once a day, compared with the validated survey, this is 42,2 %. Conclusions: Dentistry should continue to focus on the prevention of oral pathologies in vulnerable sectors such as remote or hard-to-reach communities, where supervision and professional assistance are of great relevance.

RESUMEN Introducción: la higiene bucal es fundamental para lograr una buena salud oral y así evitar múltiples patologías entre ellas la caries dental, la más frecuente. Enfermedad de origen multifactorial en la que existe la interacción de tres factores principales: el huésped (particularmente el diente y la saliva), la microflora (microorganismos) y el sustrato (dieta). Objetivo: determinar el nivel de higiene bucal de una población del barrio “La Palmira”, de la ciudad de Salcedo, Ecuador. Métodos: investigación observacional, descriptiva de corte transversal realizada en el período mayo - octubre de 2023. El universo estuvo integrado por 1200 personas, la muestra obtenida a través de muestreo aleatorio simple, según los criterios de inclusión y exclusión quedó conformada por 291 personas. Resultados: de la población estudiada, el 34 % indica que se cepilla los dientes dos veces al día para un 70,5 %, el 62,9 % indica que se cepilla tres veces al día o más. En cuanto al uso de medios alternativos, en este caso el enjuague bucal, el 39,9 % indica que ocasionalmente utiliza enjuague bucal comparando con la encuesta validada dio a conocer que es el 15 %, el 22 % indica que una vez al día utiliza enjuague bucal, comparando con la encuesta validada es un 42,2 %. Conclusiones: la odontología debe seguir enfocada hacia la prevención de patologías orales en sectores vulnerables como las comunidades apartadas o de difícil acceso, donde cobra gran relevancia la supervisión y asistencia profesional.

Abstract Background: exercise can increase the species and quantity of beneficial gut microbiota, enrich the diversity of microflora, and promote the development of symbiotic bacteria, especially in the stage of ontogeny. However, there is little evidence of the short-term voluntary exercise effect on the gut microbiota in developing mice. Material and method: therefore, we used short-term voluntary wheel running model to study the gut microbiota of developing mice (1 month old), and detected the fecal samples by 16S rRNA gene sequencing. Results: the results showed that after 4 weeks of voluntary wheel running, the body weight of the running group was significantly lower than that of the control group. Conclusion: there was a significant separation between the running group and the control group in beta diversity measures. At the family level, the clostridiales flora of the running group was higher than that of the control group. Compared with the control group, the abundance of parabacteroides flora and anaerovorax flora increased significantly, and the abundance of anaerotruncus flora and odoribacter flora decreased significantly in the running group. These results showed that gut microbiota be affected after short-term voluntary wheel running in developing mice.

Resumen Introducción: el ejercicio puede aumentar las especies y la cantidad de microbiota intestinal beneficiosa, enriquecer la diversidad de la microflora y promover el desarrollo de bacterias simbióticas, especialmente en la etapa de ontogenia. Sin embargo, hay poca evidencia del efecto del ejercicio voluntario a corto plazo sobre la microbiota intestinal en ratones en desarrollo. Material y método: por lo tanto, utilizamos un modelo de carrera de ruedas voluntario a corto plazo para estudiar la microbiota intestinal de ratones en desarrollo (1 mes de edad) y detectamos las muestras fecales mediante la secuenciación del gen 16S rRNA. Resultados: los resultados mostraron que después de 4 semanas de carrera voluntaria con ruedas, el peso corporal del grupo de carrera fue significativamente más bajo que el del grupo de control. Conclusión: hubo una diferencia significativa entre el grupo de corredores y el grupo de control en las medidas de diversidad beta. A nivel familiar, la flora de clostridiales del grupo de corredores fue mayor que la del grupo de control. En comparación con el grupo de control, la abundancia de flora parabacteroides y flora anaerovorax aumentó significativamente, y la abundancia de flora anaerotruncus y flora odoribacter disminuyó significativamente en el grupo de carrera. Estos resultados mostraron que la microbiota intestinal se ve afectada después de la carrera voluntaria a corto plazo en ratones en desarrollo.