Resumo A porção noroeste da ecorregião Floresta Atlântica do Alto Paraná é uma das mais alteradas e fragmentadas da Mata Atlântica, da qual pouco se sabe sobre a avifauna local. Nosso objetivo foi descrever a diversidade e composição da avifauna aquática, bem como analisar os padrões de similaridade quanto a distribuição temporal e espacial destas aves nesta ecorregião. Utilizamos a transecção linear para amostragem em seis áreas úmidas (corpos d’água lênticos e lóticos), nos períodos de seca e chuva entre 2012 e 2013. Registramos 52 espécies de aves aquáticas e as riquezas das áreas mostraram-se distintas, pois apenas Cairina moschata (Linnaeus, 1758), Tigrisoma lineatum (Boddaert, 1783), Rosthramus sociabilis (Vieillot, 1817), Aramus guarauna (Linnaeus, 1766), Vanellus chilensis (Molina, 1782), Jacana jacana (Linnaeus, 1766), and Arundinicola leucocephala (Linnaeus, 1764) foram comuns às seis áreas, o que indica seleção de habitat. Quando analisada a composição das aves nos dois períodos aliada à distribuição espacial, encontramos dissimilaridades temporais acentuadas entre os ambientes com características lóticas (rio e aterro) e lênticas (lagoas). Isto mostra que, além das diferentes épocas sazonais, é necessário analisar separadamente os diferentes tipos de áreas úmidas. Por fim, apesar da extensão pequena e baixa riqueza total, a área amostrada abrigou 1/3 das aves aquáticas de água doce para o estado de São Paulo, cinco espécies migratórias e 11 novas espécies para o noroeste do estado. A heterogeneidade de ambientes aquáticos locais, forte seleção de habitat aliada à sazonalidade e ausência de outros locais úmidos em seu entorno, explicam a diversidade e distribuição destas aves estreitamente relacionadas aos corpos d’água desta desconhecida ecorregião da Mata Atlântica.

Abstract The northwestern portion of the Upper Paraná Atlantic Forest ecoregion is one of the most disturbed and fragmented areas in the Atlantic Forest, and little is known about the local avifauna. In this study, we have described the composition and diversity of the aquatic avifauna of this region and analyzed the patterns of similarity with respect to the seasonal as well as spatial distribution. We used the line transect sampling technique in six distinct humid areas (including lentic and lotic water bodies) during the dry and rainy seasons of 2012 and 2013. A total of 52 species of waterfowl were recorded. The species richness of the studied areas was surprisingly distinct; only seven waterfowl species, namely Cairina moschata (Linnaeus, 1758), Tigrisoma lineatum (Boddaert, 1783), Rosthramus sociabilis (Vieillot, 1817), Aramus guarauna (Linnaeus, 1766), Vanellus chilensis (Molina, 1782), Jacana jacana (Linnaeus, 1766), and Arundinicola leucocephala (Linnaeus, 1764), were common to these six studied areas. This indicated that the other bird species that were observed might be habitat selective. Moreover, the analysis of the composition of birds in the two seasons (dry and rainy) combined with their spatial distributions showed significant dissimilarities between the areas with lotic (river and constructed wetland) and lentic (lagoons) characteristics. Nevertheless, despite the small extent and low total richness of the entire study area, it was found to be home to 1/3 of all freshwater aquatic birds documented in the state of São Paulo, with the record of 5 migratory species and 11 new species added to the northwest of the state. The heterogeneity of local aquatic environments, habitat selection combined with seasonality, and the absence of other humid locations in the surroundings can explain the diversity and distribution of these birds in the water bodies of this uninvestigated Atlantic Forest ecoregion.

Abstract The demand for low-cost and time-efficient biomonitoring and ecological assessment strategies has increased due to threats to biodiversity. We evaluated the ability of surrogates (genera and biovolume classes) to access information provided by diatom species. Moreover, we investigated whether the relative importance of spatial and environment on diatom communities identified at the genus or classified at biovolume classes reflects the ecological responses at the species. We used biotic, environmental, and spatial data from Pampa streams. We employed Procrustes analysis to detect whether the spatial variation of communities at the species level was represented by the surrogates and variation partitioning to evaluate the relative importance of spatial and environment. We found high congruence between the surrogates and diatom species communities. Furthermore, whilst both spatial and environment were important to explain species composition, only the environment was important for both surrogates. We suggest that the use of genus-level determinations can provide information about the environmental relationships of species, whereas biovolume classes may be useful to detect changes in diatom communities. These results are a first step to seek for strategies aiming to simplify biological monitoring and assessments at pampean streams, especially considering the demand for the identification of organisms at the species. lowcost low cost timeefficient time efficient biodiversity genera Moreover biotic streams Furthermore composition genuslevel

Abstract Organisms can respond to environmental gradients from local to landscape features. Aquatic insects are particularly affected by watershed peculiarities due to their dependence on microhabitat conditions. However, these relationships are poorly understood in lotic ecosystems of subtropical grasslands, limiting water resources management and bioassessment proposals. Here, we investigated how local stream environment and variations in landscape types affect the assemblage structure of a bioindicator insect group, face to the spatial proximity of the sampled locations. We sampled immatures of Ephemeroptera, Plecoptera, and Trichoptera in streams along the Brazilian Pampa biome, recording environmental descriptors in different grassland ecosystem types. The structure of aquatic insect assemblages differed across grassland types, with specific dominant genera associated with each landscape. Spatially-structured water physicochemical descriptors explained a significant amount of variation in assemblage data. Our findings suggest that grassland ecosystem type delimitations capture ecological attributes, influencing watershed features important to EPT assemblage structuration. Moreover, we highlight the importance of niche-based process structuring EPT assemblages along grassland ecosystem types of Pampa biome. In addition, we encourage using aquatic insects in bioassessment of lotic waters to assess local and landscape environmental impacts. We strongly recommend considering the grassland ecosystem schedule for water resources management and bioassessment proposals. conditions However grasslands proposals Here group locations Ephemeroptera Plecoptera biome Spatiallystructured Spatially structured data attributes structuration Moreover nichebased niche based addition impacts

Resumo A utilização de índices é indicada no monitoramento e avaliação permanente de ecossistemas aquáticos, pois eles sintetizam a complexidade técnica do resultado das variáveis e traduzem esses resultados em classes de qualidade, refletindo ações implementadas e apontam caminhos para a recuperação e conservação de recursos. Índices do estado trófico do ambiente fornecem uma visão sobre como nutrientes, disponibilidade de luz e outros fatores estimulam o desenvolvimento da biomassa algal contribui para o aumento do enriquecimento da condição dos sistemas aquáticos. O Índice do Estado Trófico (IET) Lamparelli diferencia ambientes lênticos (menor produtividade fitoplanctônica) e lóticos (maiores concentrações de Fósforo e menores concentrações de Clorofila-a). O Índice de Proteção da Vida Aquática (IVA) reflete a qualidade da água com base no estado trófico do ambiente, determina o grau de toxicidade para a biota e ainda indica deficiência em variáveis e condições essenciais para a proteção da vida aquática. Os índices foram aplicados a uma longa série de dados no monitoramento do Reservatório Guarapiranga, um reservatório urbano, localizado no Estado de São Paulo, sudeste do Brasil, que sofre com ocupação urbana em seu entorno e aporte de efluentes domésticos. A avaliação da série temporal de 1978 a 2021 com esses índices mostrou piora na condição trófica e de preservação da vida aquática no reservatório e em um dos tributários. Considerando que a avaliação conjunta dos dois índices fornece um enfoque de conservação ambiental, sua utilização em longo prazo refletiu alterações e impactos ao ambiente, mostrando ainda os locais mais conservados. Os resultados mostraram que os dois índices são indicados para aplicação em uma rede básica em ambientes subtropicais, aptos a indicar locais para aprimoramento do monitoramento e sensíveis às alterações ambientais. aquáticos recursos nutrientes IET (IET menor fitoplanctônica maiores Clorofilaa. Clorofilaa Clorofila . Clorofila-a) IVA (IVA Guarapiranga urbano Paulo Brasil domésticos 197 202 tributários ambiental conservados subtropicais ambientais Clorofila-a 19 20 1 2

Abstract The use of indices is recommended for continuous monitoring and assessment of aquatic ecosystems, as they summarize the technical complexity of the results of multiple analyzes performed and translate these results into quality classes that reflect the actions taken and indicate ways to recover and conserve the resources. Environmental trophic state indices provide information on how nutrients, light availability and other factors promote the development of algal biomass and contribute to increased enrichment status of aquatic systems. Lamparelli's Trophic State Index (TSI) distinguishes between lentic (lower phytoplankton productivity) and lotic (higher phosphorus concentrations and lower chlorophyll-a concentrations) environments. The Aquatic Life Protection Index (ALPI) reflects water quality based on the trophic state of the environment, determines the degree of toxicity to biota and indicates deficiencies in variables and conditions essential for the protection of aquatic life. The indices were applied to a long data series to monitor the Guarapiranga Reservoir, an urban reservoir in the state of São Paulo in southeastern Brazil, which suffers from the urbanization of its surroundings and the discharge of domestic wastewater. The evaluation of the time series from 1978 to 2021 using these indices showed a deterioration in the trophic status and conservation of aquatic life in the reservoir and in one of its tributaries. Considering that the joint assessment of the two indices provides an approach to environmental conservation, their long-term use reflected changes and impacts on the environment and showed the best-preserved sites. Both indices are suitable for application in a baseline network in subtropical environments. They can pinpoint locations for better monitoring and are sensitive to environmental changes. ecosystems resources nutrients systems Lamparellis Lamparelli s TSI (TSI productivity higher chlorophylla chlorophyll environments ALPI (ALPI Reservoir Brazil wastewater 197 202 tributaries longterm term bestpreserved best preserved sites 19 20 1 2

Abstract The spatial gradients created after the installation of large reservoirs produce a great diversity of biotopes, which affects the distribution and structuring of aquatic communities, mainly fish. Thus, we hypothesized that the fishes living in the lotic stretch (river zone, closest to the natural environment) of the reservoir would have lower overlap and higher niche breadth than fishes living in the lentic stretch. Samples were collected in six sections (lentic and lotic) of the Chavantes Reservoir, middle Paranapanema River. A total of 1478 individuals distributed in 13 species were sampled in both stretches. Many resources were acquired by species, and we observed that nine species present significant differences when comparing the two stretches. Furthermore, only \emph{Schizodon nasutus biotopes communities fish Thus river zone environment Reservoir River 147 1 stretches Furthermore \emphSchizodon emphSchizodon \emph Schizodon emph 14

Abstract To characterize the diet composition of Moenkhausia bonita and its temporal and ontogenetic variations in streams in the Formoso River basin (MS). The collections were carried out in seven sampling points in two periods throughout the year (dry and rainy). The food items were analyzed according to the volumetric and occurrence frequency methods and the diet was characterized through the Food Index (IAi%). To determine ontogeny, the specimens were divided into five size classes in the dry (D1 to D5) and rainy (R1 to R5) periods. To verify the difference between the species’ diet between the size classes and the periods of the year, the Permutational Multivariate Analysis of Variance – PERMANOVA analysis was performed. Moenkhausia bonita was classified as an invertivore when it consumed basically both aquatic and terrestrial invertebrates (99.5% of the diet), with higher consumption of aquatic invertebrates. There was a significant difference in the diet of between the dry and rainy periods, and although the species basically consumed the same items in the two studied periods, the proportions were different and there was no difference in the diet between size classes. M. bonita diet is based on autochthonous resources regardless of the size class, but that there were different consumption patterns when comparing the different periods of the year. The present study provided the first information on the feeding of M. bonita in a lotic environment and diet spectrum in the developmental phases, (ontogeny) and periods of the year, enabling a better understanding of the species, the importance of invertebrates in its diet, and the need for future studies on the biology, autoecology, and behavior of this species. MS. MS . (MS) rainy. rainy) IAi%. IAi IAi% (IAi%) ontogeny D1 D (D D5 R1 R (R R5 performed 99.5% 995 99 5 (99.5 , diet) M class phases (ontogeny biology autoecology (MS (IAi% 99.5 9 (99. (IAi 99. (99 (9 (

Resumo Caracterizar a composição alimentar de Moenkhausia bonita e as variações temporais e ontogenéticas na dieta desta espécie em riachos da bacia do rio Formoso (MS). As coletas foram realizadas em sete pontos amostrais em dois períodos do ano (seco e chuvoso). Os itens alimentares foram analisados de acordo com os métodos volumétrico e de frequência de ocorrência e a dieta foi caracterizada através do Índice Alimentar (IAi%). Para determinar a ontogenia, os espécimes foram divididos em cinco classes de tamanho nos períodos seco (D1 a D5) e chuvoso (R1 a R5). Para verificar a diferença entre a dieta da espécie entre as classes de tamanho e os períodos do ano foi realizado a Análise de Variância Multivariada Permutacional – PERMANOVA. M. bonita foi classificada como invertívora ao consumir basicamente invertebrados tanto aquáticos quanto terrestres (99,5% da dieta), com consumo maior de invertebrados aquáticos. Houve diferença significativa na dieta entre os períodos seco e chuvoso, apesar da espécie consumir basicamente os mesmos itens nos dois períodos estudados, as proporções foram distintas e não houve diferença na dieta entre as classes de tamanho. A dieta de M. bonita é baseada em recursos autóctones independente da classe de tamanho, mas que houve consumo diferente entre os períodos do ano. O presente estudo forneceu as primeiras informações sobre a alimentação de M. bonita em ambiente lótico e seu espectro alimentar nas fases de desenvolvimento(ontogenia)e períodos do ano, possibilitando melhor conhecimento da espécie, a importância dos invertebrados em sua dieta e a necessidade de estudos futuros sobre a biologia, autoecologia e comportamento desta espécie. MS. MS . (MS) chuvoso. chuvoso) IAi%. IAi IAi% (IAi%) ontogenia D1 D (D D5 R1 R (R R5. R5 R5) PERMANOVA M 99,5% 995 99 5 (99,5 dieta, , dieta) estudados desenvolvimentoontogeniae desenvolvimento biologia (MS (IAi% 99,5 9 (99, (IAi 99, (99 (9 (

Abstract Functional diversity is an emergent approach in ecology that has been applied globally to better understand the relationships between organisms and the environment. However, assessing the functional diversity of freshwater fish is a challenge for scientists. Aiming to summarize the evolution of scientific knowledge on fish functional diversity, we performed a systematic review of the literature published from 1945 to 2021 using the Web of Science. Based on the 101 articles reviewed, we found that publications about functional diversity of fishes have increased over time, mainly in Neotropical, Indomalayan and Palearctic regions. Most studies were conducted in lotic ecosystems, especially to assess environmental impacts such as biological invasions and land use. Functional diversity has been assessed mainly by morphological traits that reflect feeding and locomotion dimensions. Functional richness was the most common index used in the studies. Our findings suggest that political neglect and lack of investments may hamper the research development in several places. The missing information about the functional traits of many species may limit the use of the functional approach. We also highlight the need for the incorporation of functional diversity in conservation programs once functional diversity is a key facet of biodiversity to maintain freshwater ecosystem functioning. environment However scientists 194 202 Science 10 reviewed time Neotropical regions ecosystems dimensions places functioning 19 20 1 2

Resumo A diversidade funcional tem sido avaliada principalmente através de traços funcionais morfológicos que refletem as dimensões da alimentação e locomoção. A Riqueza Funcional foi a métrica mais comumente usada nos estudos. Esses resultados sugerem que a negligência política e a falta de investimentos podem dificultar o desenvolvimento de pesquisas em diversas regiões. A falta de informações sobre os traços funcionais de muitas espécies limita o uso da abordagem funcional. Destaca-se também a necessidade de incorporar a diversidade funcional em programas de conservação, uma vez que a diversidade funcional é uma faceta chave da biodiversidade para manter o funcionamento dos ecossistemas de água doce. locomoção estudos regiões Destacase Destaca se conservação doce

Abstract The success of Procamallanus (Spirocamallanus) inopinatus infection in fish involves a complexity of variables. This study aims to evaluate the relationship between abundance of P. (S.) inopinatus with biometric and somatic parameters, sex, relative condition factor (Kn) and hosts diet, as well as to evaluate length relationship of the parasites and the hosts. The fishes were collected by the mesh method and data, length, weight, sex, gonad and liver weight, Gonadosomatic index (GSI) and hepatosomatic index (HSI), Kn and stomach content were recorded. Twenty-seven specimens of P. (S.) inopinatus were collected in the intestine from Serrasalmus rhombeus and 52 from Leporinus friderici. In general, the prevalence, mean intensity and mean abundance of infection was higher in L. friderici. The total abundance was explained by the variables GSI, HSI total length, gonad and liver weight. Fish relative condition factor (kn) and sex were not influenced by the infection, being that the parasite infection did not impair the body condition of the hosts. There is no relationship between host length and parasite length in any of the evaluated fish species. On average, S. rhombeus parasites are 0.69 cm larger than L. friderici parasites. Spirocamallanus (Spirocamallanus P S (S. parameters (Kn diet data weight GSI (GSI HSI, , (HSI) recorded Twentyseven Twenty seven 5 general prevalence L kn (kn species average 069 0 69 0.6 (S (HSI 06 6 0.

Abstract: Aim Additive diversity partitioning has been used to explain the accumulation of diversity at different spatial scales with relative success. In lotic ecosystems, the spatial extent is extremely relevant in studies of diversity accumulation, because it encompasses environmental variation that causes changes in the observed communities. Despite of previous knowledge on the effect of extent on biological communities and diversity accumulation, little is known about the topic in aquatic insect communities in large rivers. In this context, we studied the effect of spatial extent and environmental variation on diversity components, alpha and beta, in Ephemeroptera, Plecoptera and Trichoptera (EPT) groups in Xingu River rapids. Methods The sampling was carried out in October 2015 in the dry period of the region, in nine rapids in the Xingu, Bacajá and Iriri rivers. At each collection site, five Surber samples were taken. We also recorded pH, dissolved oxygen, electrical conductivity, water temperature, and geographic coordinates. We used additive diversity partitioning to separate the diversity components α and β. For the spatial component, we generated the spatial filters using PCNM (Principal Coordinates of Neighbour Matrices) and partitioned the variance between space and environment using partial Redundancy Analysis (pRDA). Results We collected 12,249 individuals in 27 genera within 11 families in the EPT orders. The greatest accumulation of diversity was observed among rapids of the river, when the β diversity in this spatial extent was greater than the expected. The spatial structure was an indirect effect at this extent, since it is a relevant drive to environmental variables. Conclusions The results indicate that the effect of spatial extent on rapids is a contributing factor in the diversity components of aquatic insect communities in large river rapids. To the conservation and management of this environment is necessary cover as many rapids as possible, since the preservation of only a few rapids can mean a substantial loss of regional diversity. Abstract success ecosystems rivers context beta Ephemeroptera (EPT 201 region site taken pH oxygen conductivity temperature coordinates component Principal Matrices pRDA. pRDA . (pRDA) 12249 12 249 12,24 2 1 orders expected variables possible 20 (pRDA 1224 24 12,2 122 12,

Resumo: Objetivo O particionamento aditivo da diversidade tem sido utilizado para explicar a acumulação de diversidade em diferentes escalas espaciais com relativo sucesso. Em ecossistemas lóticos, a escala espacial é extremamente relevante em estudos de acumulação de diversidade, pois engloba a variação ambiental que causa mudanças nas comunidades observadas. Apesar do conhecimento prévio sobre o efeito da escala nas comunidades biológicas e na acumulação de diversidade, pouco se sabe sobre o tema em comunidades de insetos aquáticos em grandes rios. Neste contexto, estudamos o efeito da escala espacial e da variação ambiental sobre os componentes da diversidade, alfa e beta, em grupos de Ephemeroptera, Plecoptera e Trichoptera (EPT) em corredeiras do rio Xingu. Métodos A amostragem foi realizada em outubro de 2015, no período seco da região, em nove corredeiras nos rios Xingu, Bacajá e Iriri. Em cada local de coleta, foram coletadas cinco amostras de Surber. Também foram registradas as variáveis pH, oxigênio dissolvido, condutividade elétrica, temperatura da água e coordenadas geográficas. Usamos o particionamento aditivo da diversidade para separar os componentes de diversidade α e β. Para o componente espacial, geramos os filtros espaciais usando PCNM (Principal Coordinates of Neighbour Matrices) e particionamos a variância entre espaço e ambiente usando a análise de redundância parcial (pRDA). Resultados Foram coletados 12.249 indivíduos em 27 gêneros dentro de 11 famílias nas ordens EPT. O maior acúmulo de diversidade foi observado entre as corredeiras do rio, quando a diversidade β nessa escala espacial foi maior que a esperada. A estrutura espacial foi um efeito indireto nesta escala, uma vez que é um direcionador relevante para as variáveis ambientais. Conclusões Os resultados indicam que o efeito da escala espacial em corredeiras é um fator contribuinte nos componentes de diversidade das comunidades de insetos aquáticos em corredeiras de grandes rios. Para a conservação e manejo desse ambiente é necessário abranger o maior número possível de corredeiras, uma vez que a preservação de apenas algumas corredeiras pode significar uma perda substancial da diversidade regional. Resumo sucesso lóticos observadas contexto beta Ephemeroptera EPT (EPT Xingu 2015 região Iriri coleta Surber pH dissolvido elétrica geográficas Principal Matrices pRDA. pRDA . (pRDA) 12249 12 249 12.24 2 1 esperada ambientais regional 201 (pRDA 1224 24 12.2 20 122 12.

Resumo Evidências indicam que os peixes migradores se reproduzem no médio rio Uruguai, mas a localização dos locais de desova ainda permanece desconhecida. Para identificar estes locais, foram realizadas mensalmente entre outubro de 2016 e janeiro de 2017, amostragens de ovos e larvas de peixes em três seções ao longo do médio rio Uruguai, cobrindo dois ambientes: o canal principal e a foz dos afluentes (rios Comandaí, Ijuí e Piratinim). Foram capturados 11.519 ovos e 3.211 larvas, pertencentes a dez espécies migradoras. Ovos e larvas de peixes migradores, foram amplamente distribuídos, com predomínio de ovos segmentados e larvas em estágios de larval-vitelino e pré-flexão, com maiores densidades próximo à confluência com o rio Piratinim. As assembleias de larvas mostraram variação espacial, indicando que os locais de desova diferem entre as espécies migradoras. Este estudo fornece novas informações sobre a reprodução de peixes no médio rio Uruguai, revelando que espécies migradoras desovam em diferentes localidades ao longo do canal principal e afluentes. Esta região pode funcionar como um local crítico para a reprodução de peixes, embora atualmente esteja ameaçada pelo risco de expansão da energia hidrelétrica, enfatizando a necessidade de medidas que preservem suas condições ambientais, conectividade hidrológica e funções ecológicas.

Abstract Evidence indicates that migratory fish reproduce in the middle Uruguay River, but the location of spawning sites remains unknown. To identify spawning sites in the basin, fish eggs and larvae were sampled monthly between October 2016 and January 2017. The sampling was carried out in three sections along the middle Uruguay River, covering two environments: the main channel and the mouth of tributaries (Comandaí, Ijuí, and Piratinim rivers). A total of 11,519 eggs and 3,211 larvae were captured, belonged to ten migratory species. Eggs and larvae of migratory fishes, were widely distributed, with predominance of segmented eggs, and larvae in yolk-sac and pre-flexion stages, with higher densities near the confluence with the Piratinim River. Larvae assemblages showed spatial variation, indicating that spawning sites differ among migratory species. This study provides new information about fish reproduction in the middle Uruguay River, revealing that migratory species spawn in different localities along the main channel and tributaries. This region may function as a critical site for fish reproduction, although it is currently threatened by the risk of hydropower expansion, emphasizing the need for measures that preserve their environmental conditions, hydrological connectivity and ecological functions.

Abstract Patterns of beta diversity of plankton communities in rivers have been mainly determined by hydrological factors that alter the dispersion and composition of species and traits. Rotifers in the Guamá River (eastern Amazonian River) were sampled (monthly between October 2017 and June 2019) to analyze the temporal variation of taxonomic and functional beta diversity and its partitions (turnover and nestedness) as well as the effects of temporal, environmental, and seasonal dissimilarities. Taxonomic turnover and functional nestedness over time were observed as well as functional homogenization, which was arguably due to the hypereutrophic condition of the river. There were no seasonal differences in taxonomic and functional beta diversity probably due the low environmental dissimilarity. This study demonstrated that this Guamá River stretch presented low environmental dissimilarity and hypereutrophic waters, which benefited the establishment of a community of species with high taxonomic turnover over time, but with low functional dissimilarity and loss of some functions related to the functional traits evaluated in the ecosystem. It is important to point out that temporal studies should evaluate both taxonomic and functional aspects of communities, mainly because the effect of environmental changes may be more noticeable at the functional level of communities.

Resumen Introducción: La concentración de clorofila a representa la biomasa de fitoplancton la cual influye directamente en las funciones de producción de los ecosistemas acuáticos. Por lo tanto, es imperativo comprender su cinética espacio-temporal en el ambiente lótico con respecto a las variabilidades climáticas regionales en las aguas continentales tropicales. Objetivo: Se realizaron estudios in situ para examinar la influencia de varios parámetros ambientales en la biomasa del fitoplancton en la cuenca baja del Ganges durante 2014-2016. Métodos: En primer lugar, se determinaron los parámetros ambientales más influyentes en la concentración de Chl-a fluvial. Luego, el efecto directo en cascada de las variables climáticas sobre los parámetros ambientales clave, mediante el modelado y los cambios en la concentración media de Chl-a en el tramo inferior del río. Resultados: Solo cinco parámetros ambientales, entre ellos, temperatura del agua, sólidos disueltos totales, salinidad, alcalinidad total y pH, fueron factores clave que influyeron en la Chl-a (R2 múltiple: 0.638, P < 0.05). Las estimaciones actuales indican que si la tasa actual de variabilidad climática regional durante las últimas 3 décadas (temperatura media del aire + 0.24 °C, precipitación total anual -196.3 mm) permanece constante durante las próximas tres décadas (2015-2045), se presente un aumento en el promedio de la Chl-a en +170 µgL-1 y alcance aproximadamente 475.94 µgL-1 para el 2045 o más. Conclusiones: Este estudio presenta una metodología basada en modelos alternativos en situaciones de escasez de datos, la información generada también podría contribuir a mejorar la gobernanza del agua y a desarrollar un protocolo para la gestión sostenible del agua y de esta manera mejorar los servicios ecosistémicos.

Abstract Introduction: Chlorophyll a concentration proxies the phytoplankton biomass which directly involves in signifying the production functions of aquatic ecosystem. Thus, it is imperative to understand their spatio-temporal kinetics in lotic environment with reference to regional climatic variabilities in the tropical inland waters. Objective: In-situ studies were conducted to examine the changes in phytoplankton biomass in lower Ganga basin as influenced by various environmental parameters under regional climatic variability during 2014-2016. Methods: Firstly, the most key influential environmental parameters on riverine Chl-a concentration were determined. Then the direct cascading effect of changing climatic variables on key environmental parameters were derived through modeling and quantified probable changes in mean Chl-a concentration in the lower stretch of river. Results: Only five environmental parameters namely water temperature, total dissolved solid, salinity, total alkalinity and pH were key factors influencing Chl-a (Multiple R2: 0.638, P < 0.05). Present estimates indicate that if the present rate of regional climatic variability over the last 3 decades (mean air temperature + 0.24 °C, total annual rainfall -196.3 mm) remain consistent over the next three decades (2015-2045), an increase in mean Chl-a by + 170 µgL-1 may likely be expected grossly reaching about 475.94 µg L-1 by the year 2045 or more. Conclusions: The present study is first such comprehending a gross hint towards the probable ecosystem response with an alternative model based methodology in data-deficient situations. Subsequently, the output would also be of great benefit for increase water governance and developing strategy protocol for sustainable water management for greater ecosystem services.

RESUMO Os protozoários Giardia spp. e Cryptosporidium spp. são agentes etiológicos responsáveis pela transmissão de gastroenterites, principalmente por causa do consumo de água contaminada. Seus (oo)cistos são resistentes às condições ambientais adversas, bem como à maioria dos processos convencionais de tratamento da água. Assim, o objetivo deste trabalho foi avaliar a ocorrência de (oo)cistos desses protozoários em águas superficiais captadas para consumo humano no estado de Goiás, Brasil. Quinze amostras de água bruta foram coletadas para avaliar a ocorrência de (oo)cistos dos protozoários pelo método de Filtração por Membrana, além da análise de turbidez e pH. As taxas de recuperação nos testes com água ultrapura atingiram os critérios da USEPA (2012) para Giardia (78.1 ± 0%) e para Cryptosporidium (60.6 ± 32.6%), entretanto, em água bruta, a recuperação foi inferior devido à turbidez. Todas as amostras (n = 15) foram positivas para oocistos de Cryptosporidium spp., com valor máximo de 250 oocistos/L, demonstrando que esses protozoários se disseminam no meio aquático do estado de Goiás e geram risco à saúde pública. Devido à utilização de mananciais para consumo público, recomenda-se que as autoridades públicas e as empresas de saneamento atuem na preservação e manutenção dos cursos d’água, realizem o monitoramento periódico das plantas de tratamento que realizam adução no Córrego Cerrado, Córrego Santana e Córrego São Manoel e aprimorem as tecnologias de tratamento existente. Os resultados não permitiram inferir se a carga de animais e a área de pastagem promove o aumento da contaminação dos sistemas aquáticos lóticos.

ABSTRACT The protozoa Cryptosporidium spp. and Giardia spp. are etiological agents responsible for the transmission of gastroenteritis, mainly due to the consumption of contaminated water. Their (oo)cysts are resistant to adverse environmental conditions, as well as to most conventional water treatment processes. Thus, the objective of this work was to evaluate the occurrence of (oo)cysts of these protozoans in surface water collected for human consumption in the state of Goiás, Brazil. Fifteen samples of raw water were collected to assess the occurrence of (oo)cysts of the protozoa using the Membrane Filtration method, in addition to turbidity and pH analyses. Recovery rates in tests with ultrapure water reached the USEPA (2012) criteria for Giardia (78.1% ± 0%) and for Cryptosporidium (60.6% ± 32.6%); however, recovery in raw water was lower due to turbidity. All samples (n = 15) were positive for Cryptosporidium spp. oocysts, with a maximum concentration of 250 oocysts/L, demonstrating that these protozoa are disseminated in the aquatic environment of the state of Goiás and pose a risk to public health. Due to the use of water sources for public consumption, it is recommended that public authorities and sanitation companies act to preserve and maintain water courses, carry out periodic monitoring of treatment plants that supply the Cerrado, Santana and São Manoel streams and improve existing treatment technologies. The results did not allow to infer whether animal load and grazing area promote an increase in contamination of the lotic aquatic systems.

Resumo Neste estudo, determinamos os principais parâmetros reprodutivos do piau gordura, Leporinus piau, em duas seções da bacia do rio São Francisco. Entre maio de 2015 e abril de 2016, um total de 573 espécimes foram capturados de um ambiente lêntico (seção 1), o reservatório de Três Marias (RTM), e um ambiente lótico (seção 2), à jusante da RTM na confluência do rio São Francisco (RSF) com o rio Abaeté. A análise da atividade reprodutiva mostrou que L. piau se reproduz nas duas seções, mas fêmeas e machos da seção 1 apresentaram maiores valores de comprimento total, peso corporal, fator de condição de Fulton e índice gonadossomático em comparação com a seção 2. O dimorfismo sexual foi evidente na espécie com as fêmeas sendo maiores do que os machos. Além disso, os machos atingiram a primeira maturação gonadal em tamanho menor do que as fêmeas. O pico do estágio de maturação/maduro foi observado em novembro/abril para fêmeas e machos na seção 1 e em novembro/dezembro na seção 2, coincidindo com altas temperaturas e precipitação na região. Em ambas as seções do rio, L. piau apresentou características típicas de desova parcelada com período de desova prolongada e reproduz preferencialmente em ambientes lênticos.

Abstract In this study, we determined the main reproductive parameters of piau gordura, Leporinus piau, in two sections of the São Francisco River basin. Between May 2015 and April 2016, a total of 573 specimens were captured from a lentic environment (section 1), the Três Marias Reservoir (TMR), and a lotic environment (section 2), downstream of the TMR at the confluence of the São Francisco River (SFR) with the Abaeté River. Analysis of reproductive activity showed that L. piau from both sections reproduced, but females and males from section 1 exhibited higher total length, body weight, Fulton condition factor, and gonadosomatic index values, as compared to section 2. Sexual dimorphism was evident in the species, with females being larger than males. Moreover, males reached first gonadal maturation at a smaller size than females. The peak maturation/mature stage was observed in November/April for females and males in section 1 and in November/December in section 2, coinciding with high temperatures and precipitation in the region. In both sections of the river, L. piau exhibited the typical characteristics of partial spawning, with a prolonged spawning period, and preferential reproduction in lentic environments.

Resumo O presente estudo objetivou inventariar a biodiversidade de microcrustáceos planctônicos (Cladocera e Copepoda) em 44 mananciais da região semiárida do Brazil, ampliando o conhecimento da comunidade zooplanctônica desta região, incluindo as espécies fitófilas. Os organismos foram coletados de modo não sistemático entre 2011 e 2017, nos estados do Rio Grande do Norte, Pernambuco, Ceará e Paraíba. Um total de 60 espécies de microcrustáceos planctônicos foram identificadas, sendo 52 de cladóceros e oito de copépodes. A bacia que apresentou maior riqueza foi a do rio Pajeú com 41 espécies. Mesmo assim, o número extrapolado de espécies mostra tendência de aumento para essa bacia. A composição dos gêneros foi diferente entre as bacias hidrográficas, sendo a bacia do Moxotó (Pernambuco) isolada das demais, talvez porque nesta bacia as lagoas foram o único tipo de ambiente amostrado. Os padrões de riqueza e de abundância se mostraram mais elevados para as bacias ondem a amostragem foi realizada principalmente em ambientes lênticos, como a do Pajeú e do Moxotó. Inventários de microcrustáceos e estudos de longa duração precisam ser maximizados para ampliação do conhecimento da biodiversidade dos ecossistemas aquáticos continentais da região semiárida brasileira.

Abstract The present study aimed to inventory the biodiversity of planktonic microcrustaceans (Cladocera and Copepoda) in 44 environments from the semiarid region of Brazil, increasing the knowledge about zooplankton community, including phytophilous species. Between 2011 and 2017, organisms were collected in a non - systematic way in the states of Rio Grande do Norte, Pernambuco, Ceará and Paraíba. A total of 60 species of planktonic microcrustaceans were identified, of which 52 were cladocerans and eight were copepods. The basin that presented the highest richness was Pajeú River with 41 species. Nevertheless, the extrapolated number of species shows an increasing tendency for this basin. The genus composition was different between the basins, with Moxotó basin (Pernambuco) being isolated from the others, probably since lagoons were the only type of environment sampled in this basin. Richness and abundance patterns were higher for the basins where sampling was mainly performed in lentic environments, such as Pajeú and Moxotó. Microcrustacean inventories and long-term studies need to be conducted more frequently to better understand the biodiversity of continental aquatic ecosystems in the semiarid region of Brazil.