Compared to other species, components of Combretum lanceolatum are still with few data reported in the literature. This work describes the compounds identification, antioxidant and antimicrobial activities of C. lanceolatum twigs. Ethanolic extract (EECLT) from C. lanceolatum twigs was submitted to partition obtaining: hexane (HF), diethyl ether (EEF), ethyl acetate (EAF) and hydromethanolic (HMF) fractions. They were analyzed by gas chromatography-mass spectrometry, high performance liquid chromatography coupled to mass spectrometry and direct insertion mass spectrometry and evaluated for antioxidant assay (2,2-diphenyl-1-picrylhydrazyl (DPPH) method) and antimicrobial assay. Fifty-seven compounds were identified in the fractions. In antioxidant assay, EECLT and EAF demonstrated good antioxidant potential (half maximal effective concentration (EC50): 57.9 and 45.4 µg mL–1, respectively), better than the positive control butylated hydroxytoluene (BHT) (EC50: 69.34 µg mL–1). For antimicrobial assay, all fractions presented promising minimal inhibitory concentration (MIC). EECLT presented the best values against Staphylococcus aureus (125 µg mL–1) and Escherichia coli (62.5 µg mL–1) and better than the positive control fluconazole (8 and 16 µg mL–1) against Candida albicans (3.9 µg mL–1) and Candida krusei (15.6 µg mL–1) strains. This study presents relevant chemical and biological information about C. lanceloatum expanding the knowledge of Brazilian flora. species literature identification C (EECLT obtaining HF, HF , (HF) EEF, EEF (EEF) (EAF HMF (HMF chromatographymass 2,2diphenyl1picrylhydrazyl 22diphenyl1picrylhydrazyl diphenylpicrylhydrazyl 2,2 diphenyl 1 picrylhydrazyl 2 DPPH (DPPH method Fiftyseven Fifty seven half EC50 EC (EC50) 579 57 9 57. 454 45 4 45. mL1 mL mL–1 respectively, respectively respectively) BHT (BHT (EC50 6934 69 34 69.3 mL–1. . MIC. MIC (MIC) 125 (12 62.5 625 62 5 (62. 8 ( 3.9 39 3 (3. 15.6 156 15 6 (15. strains flora (HF (EEF 2diphenyl1picrylhydrazyl 22 2, EC5 mL– (EC5 693 69. (MIC 12 (1 62. (62 3. (3 15. (15 (EC (6

Brazilian Savanna biodiversity is abundant in native fruits with nutritional and sensory properties with potential technological applications. However, the information available regarding the proprieties of Brazilian native fruits is still limited. This study aimed to analyze the metabolic profile of Brosimum gaudichaudii fruits (BGF) using metabolomics approaches, besides its physicochemical and nutritional proprieties. BGF exhibited protein content at least two times higher than other native fruits. Fifty-five metabolites were identified by the metabolomics approaches, mainly fatty acids (n = 22), carbohydrates (n = 13), organic acids (n = 6), and polyphenols (n = 5). The most abundant constituents were D-fructose (42.97%) for sugars, L-asparagine (6.47%) for amino acids, succinic acid (44.11%) for organic acids, and linolenic acid (3.04%) for fatty acids. Highlighted phytochemicals included chlorogenic acid, α-amyrin, and γ-tocopherol. Additionally, this study revealed that BGF is a good source of vitamin C and dietary fiber and an excellent source of vitamin A. Future studies providing nutritional information and suggesting consumption methods and potential industrial applications are essential to familiarize consumers with these foods. Additionally, clarifying the appropriate planting and harvesting seasons can revive traditional practices, especially for native fruits like BGF. However limited (BGF approaches Fiftyfive Fifty five n 22, 22 , 22) 13, 13 13) 6, 6 6) 5. 5 . 5) Dfructose D fructose 42.97% 4297 42 97 (42.97% sugars Lasparagine L asparagine 6.47% 647 47 (6.47% 44.11% 4411 44 11 (44.11% 3.04% 304 3 04 (3.04% αamyrin, αamyrin α amyrin, amyrin α-amyrin γtocopherol. γtocopherol γ tocopherol. tocopherol γ-tocopherol Additionally A foods practices 2 1 42.97 429 4 9 (42.97 6.47 64 (6.47 44.11 441 (44.11 3.04 30 0 (3.04 42.9 (42.9 6.4 (6.4 44.1 (44.1 3.0 (3.0 42. (42. 6. (6. 44. (44. 3. (3. (42 (6 (44 (3 (4 (

The inhibition of the fatty acid amide hydrolase (FAAH), an endocannabinoid system component, emerged as a potentially new therapeutic target for a range of clinical disorders such as acute and chronic pain. Some α-ketoheterocycle derivatives demonstrated interesting analgesic and anti-inflammatory activities in vitro. Ligand-Based Drug Design techniques such as knowledge graph convolutional networks (kGCN) and hologram quantitative structure-activity relationship (HQSAR) using α-ketoheterocycle derivatives from five different datasets were generated to discover the relation between the chemical structures and the inhibition activity. Meanwhile, structure-based drug design simulations as interaction fields (MIF), molecular docking, and ligand sites studies (LSI) from FAAH were performed using Autogrid software and FTmap/FTsite servers. The results of both studies were merged to propose predictive models. The resulting kGCN model demonstrated adequate accuracy area under the curve by receiver operating characteristic (AUC-ROC 0.7922). From contribution maps of the Ligand-Based Drug Design (LBDD) models and the generated probes using MIF and LSI, it was observed that the oxazole ring, the ketone group, and the apolar chain present in the structures of the inhibitors are important, besides the evidence of the Cys269 and Val270 residues importance for the potential interaction, confirmed by carried docking studies. These fragments and structural information can be used to carry out new FAAH potential inhibitors studies and report kGCN as an accurate classification technique. FAAH, , (FAAH) component pain αketoheterocycle α ketoheterocycle antiinflammatory anti inflammatory vitro LigandBased Ligand Based (kGCN structureactivity structure activity HQSAR (HQSAR Meanwhile structurebased based MIF, (MIF) LSI (LSI FTmapFTsite FTmap FTsite servers AUCROC AUC ROC 0.7922. 07922 0.7922 . 0 7922 0.7922) LBDD (LBDD ring group important Cys Cys26 Val Val27 technique (FAAH (MIF 0792 0.792 792 Cys2 Val2 079 0.79 79 07 0.7 7 0.

Hair can provide chemical information about drug use. This study employed matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) to detect drugs in hair samples. The technique was used to detect cocaine (COC, molecular weight (Mw) = 303.14 Da) and its main metabolites, benzoylecgonine (BZE, Mw = 289.13 Da), cocaethylene (COCE, Mw = 317.16 Da) and ecgonine methyl ester (EME, Mw = 199.12 Da) in hair strands using 2,5-dihydroxybenzoic acid (DHB, 7.0 mg mL-1) and α-cyano-4-hydroxycinnamic acid (CHCA, at concentrations of 1.5, 3.0, 7.0, and 10 mg mL-1) as the evaluated matrices. Among these, CHCA was selected. Subsequently, the qualitative results obtained by MALDI(+) MS were correlated with the quantitative analyses of gas chromatography-mass spectrometry (GC-MS), demonstrating a significant correlation in responses (70%), with the detection of analytes by MALDI(+) MS even at low concentrations, determined by GC-MS. The concentration ranges obtained by GC MS analysis were from 0.6 to 4.1 ng mg-1 of COC, and from 0.05 to 0.4 ng mg-1 of COCE. The effect of pre-washing on the detection of COC and its metabolites was also evaluated, and the results indicated less influence from the pre-washing stage and confirmed the reduction in COC detection in hair exposed to bleaching. use matrixassisted matrix assisted MALDIMS MALDI (MALDI-MS samples (COC (Mw 30314 303 14 303.1 Da BZE, BZE (BZE 28913 289 13 289.1 Da, , COCE, COCE (COCE 31716 317 16 317.1 EME, EME (EME 19912 199 12 199.1 2,5dihydroxybenzoic 25dihydroxybenzoic dihydroxybenzoic 2,5 2 5 DHB, DHB (DHB 70 7 0 7. mL1 mL 1 mL-1 αcyano4hydroxycinnamic αcyanohydroxycinnamic α cyano 4 hydroxycinnamic CHCA, (CHCA 15 1.5 30 3 3.0 matrices these selected Subsequently MALDI+ + MALDI(+ chromatographymass chromatography GCMS, GCMS (GC-MS) 70%, 70% (70%) GCMS. MS. GC-MS 06 6 0. 41 4. mg1 mg- 005 05 0.0 04 prewashing pre washing bleaching 3031 303. 2891 28 289. 3171 31 317. 1991 19 199. 5dihydroxybenzoic 25 2, mL- αcyano 1. 3. MALDI( (GC-MS (70% 00 (70 (7 (

Brazil is home to a rich biodiversity of native edible fruits renowned for their health-promoting compounds and flavors. Among these, Eugenia uniflora L. (Brazilian pitanga) is particularly notable. The fruit varies in color from yellow to purple and is prized for its intense aroma and versatile taste profile. However, information about the compounds responsible for their nutritional and sensory potential is still limited. This study analyzed volatile compounds and metabolites in yellow, red and purple pitanga fruits (Eugenia uniflora) at various ripening stages, exploiting a metabolomics approach. Yellow pitanga had 30 volatile compounds, with esters (40%), terpenes (26.67%) and aldehydes (16.67%) constituting 83.34% of identified volatile compounds. Red pitanga featured 26 volatile compounds, primarily terpenes and esters (each 30.77%) and aldehydes (15.38%), making up 76.92%. Purple pitanga contained 39 volatile compounds, with terpenes and esters (each 35.90%) and aldehydes (17.95%), totaling 89.75% of identified volatile compounds. Across all pitanga types, 124 metabolites were identified, including saturated fatty acids (17.74%), terpenes (17.74%) and sugars (16.93%), constituting 52.41% of identified metabolites. Future studies should provide nutritional information, consumption methods and industrial applications for pitanga fruits, focusing on sensory analysis, health benefits, preservation and breeding to enhance bioactive compounds and flavor. healthpromoting promoting flavors these L Brazilian notable profile However limited stages approach 3 40%, 40 40% , (40%) 26.67% 2667 67 (26.67% 16.67% 1667 16 (16.67% 8334 83 34 83.34 2 each 30.77% 3077 77 15.38%, 1538 15.38% 15 38 (15.38%) 7692 76 92 76.92% 35.90% 3590 35 90 17.95%, 1795 17.95% 17 95 (17.95%) 8975 89 75 89.75 types 12 17.74%, 1774 17.74% 74 (17.74% 16.93%, 1693 16.93% 93 (16.93%) 5241 52 41 52.41 analysis benefits flavor 4 (40% 26.67 266 6 (26.67 16.67 166 1 (16.67 833 8 83.3 30.77 307 7 153 15.38 (15.38% 769 9 76.92 35.90 359 179 17.95 (17.95% 897 89.7 177 17.74 (17.74 169 16.93 (16.93% 524 5 52.4 (40 26.6 (26.6 16.6 (16.6 83. 30.7 15.3 (15.38 76.9 35.9 17.9 (17.95 89. 17.7 (17.7 16.9 (16.93 52. (4 26. (26. 16. (16. 30. 15. (15.3 76. 35. 17. (17.9 (17. (16.9 ( (26 (16 (15. (17 (2 (1 (15

ABSTRACT Introduction: this study links ethical premises and foundations to analyze the dimensions involved in the design of public health policies and health reforms from a social, clinical and public health perspective. Methodology: technological resources and search platforms, applications of inclusion and exclusion criteria were used to select relevant original articles, through a systematic review of the literature, in Medline, Ibecs, Pubmed, Scielo, BVS, Scopus, Web of Science databases, in English and Spanish, about ethics from the clinical, social and public health perspective, organizing the information in scientific tables. Results: the decent living conditions of individuals from any social stratum are related to the guarantees of health care and preservation of living conditions that directly impact health and well-being, and must be supported by institutions and society. Conclusion: regulatory frameworks, connected to human rights, must guarantee equitable access to health care, without discrimination, within the framework of community participation, privacy and social dialogue, influencing policy formulation, accountability, prevention of conflicts of interest and continuous evaluation of effectiveness, leading to the highest impact of health interventions.

RESUMEN Introducción: este estudio vincula premisas y fundamentaciones éticas, para el análisis de dimensiones involucradas en el diseño de políticas públicas de salud y reformas de salud, desde la perspectiva social, del ámbito clínico y de salud pública. Metodología: se utilizaron recursos tecnológicos y plataformas de búsqueda, aplica criterios de inclusión y exclusión para seleccionar artículos originales relevantes, a través de revisión sistematizada de la literatura, en bases de datos Medline, Ibecs, Pubmed, Scielo, BVS, Scopus, Web of Science, en inglés y español, acerca de la ética desde la perspectiva clínica, social y de la salud pública, organizando la información en tablas científicas. Resultados: las condiciones de vida digna de individuos de cualquier estrato social, se relacionan a las garantías de atención sanitaria y preservación de condiciones de vida que impactan directamente en la salud y el bienestar, debiendo ser respaldados por las instituciones y la sociedad. Conclusión: los marcos regulatorios, conectados a los derechos humanos, deben garantizar el acceso equitativo a la atención a la salud, sin discriminación, en el marco de la participación comunitaria, la privacidad y el dialogo social, influyendo en la formulación de políticas, la rendición de cuentas, la prevención de conflictos de interés y la evaluación continua de la efectividad, conducentes al más alto impacto de las intervenciones en salud.

Resumen: Se trata de estudiar, en una primera aproximación, el impacto del cambio en el modo de producción como consecuencia de la telemática y el uso del aprendizaje de las máquinas y la inteligencia artificial en muchas de las aplicaciones y procesos repetitivos en los sistemas sociales de producción y distribución de bienes. Se utilizó el método del construccionismo sistémico, que, en definitiva, parte de la base de que los datos no son datos, sino construcciones con supuestos teóricos inevitables, y de que la lengua es el sistema en el que se construyen los lenguajes que son un límite general para obtener y comunicar información en la sociedad. Esto conduce a una teoría de las acciones algo diferente de las de Parsons y Luhmann, que lleva al concepto de praxemas, y a todo un nuevo análisis de la sociedad y su derecho. Resumen estudiar aproximación bienes sistémico definitiva inevitables Luhmann praxemas derecho

Abstract: The aim is to study, in a first approximation, the impact of the change in the production mode as a result of telematics and the use of machine learning and artificial intelligence in many of the applications and repetitive processes in social systems of production and distribution of goods. The method of systemic constructionism was used, which, in short, assumes that data are not data - they are constructions with unassailable theoretical assumptions and that language is the system in which the general limit languages are constructed to obtain and communicate information in society. This leads to a theory of actions somewhat different from those of Parsons and Luhmann - leading to the concept of praxemas, and to a whole new analysis of society and its law. Abstract study approximation goods used short praxemas law

Resumo: Objetiva-se estudar, em primeira aproximação, o impacto da mudança do modo de produção por efeito da telemática e do emprego do machine learning e inteligência artificial em grande parte das aplicações e processos repetitivos nos sistemas sociais de produção e distribuição de mercadorias. Utilizou-se o método do construcionismo-sistêmico que, em síntese, assume que os dados não são dados – são construções com pressupostos teóricos inafastáveis em que a língua é o sistema no qual se constroem as linguagens, limite geral da obtenção e da comunicação de informações na sociedade. Disso advém uma teoria das ações diferente daquelas de Parsons e Luhmann – conduzindo ao conceito de praxemas e, a toda uma nova analítica da sociedade e de seu direito. Resumo Objetivase Objetiva estudar aproximação mercadorias Utilizouse Utilizou construcionismosistêmico construcionismo sistêmico síntese linguagens direito

Abstract Purpose: To identify the challenges faced by nanomedicine in Mexico, according to the opinion of experts in the field. Methodological design: Semi-structured interviews were conducted with Mexican researchers in the field of nanomedicine. The participants were selected through purposive sampling. Interviews were both online and face-to-face. The information was processed with ATLAS.ti software. Results: Challenges were identified in different areas: financing, social benefit, human resources, infrastructure, nanotoxicology, patenting, preclinical and clinical research, academia-industry linkage, regulation, scientific policy and multidisciplinary work. Research limitations: Interviews included researchers only. It would be necessary to interview other agents involved in the nanomedicine chain of value, such as entrepreneurs and health sector managers. Findings: The main challenges facing nanomedical research in Mexico are the following: reduced funding, research with little impact on society, lack of specific educational programs in nanomedicine, low patenting activity, weak links between academia and industry, and lack of regulation. Some proposals to address these challenges are analyzed in this paper.

Resumen Objetivo: Identificar los desafíos que enfrenta la investigación en nanomedicina en México según la opinión de expertos en la materia. Diseño metodológico: Se realizaron entrevistas semiestructuradas a investigadores mexicanos del área de nanomedicina. Los entrevistados fueron seleccionados a través de un muestreo intencional. Las entrevistas se llevaron a cabo de manera presencial y a distancia. El procesamiento de la información se realizó con el software ATLAS.ti. Resultados: Se identificaron desafíos en distintos rubros: financiamiento, beneficio social, recursos humanos, infraestructura, nanotoxicología, patentamiento, investigación preclínica y clínica, vinculación academia-industria, regulación, política científica y trabajo multidisciplinario. Limitaciones de la investigación: Las entrevistas se realizaron únicamente a investigadores. Sería necesario entrevistar a otros agentes involucrados en la cadena de valor de la nanomedicina tales como empresarios y funcionarios del sector salud. Hallazgos: Los principales desafíos que enfrenta la investigación nanomédica en México son el financiamiento reducido, investigaciones con poca incidencia en la sociedad, inexistencia de programas educativos específicos en nanomedicina, escaso patentamiento, nula vinculación con empresas y falta de regulación. Algunas propuestas para encarar estos desafíos se analizan en el presente trabajo.

ABSTRACT Objective: To describe the evidence of Platelet Rich Plasma (PRP), Stem cells therapy (SCT) and Extracorporeal shockwave therapy (ESWL) for the treatment of Peyronies disease (PD), including information from the main urological society guidelines. Materials and Methods: A literature review of PubMed articles published between 2000 and 2023 was conducted, utilizing keywords such as "Peyronie's Disease", "Penile curvature", "Platelet Rich Plasma", "Stem cells", and "Extracorporeal shockwave therapy". Only full-text articles in English were included, excluding case reports and opinions. Results: A considerable number of clinical trials were conducted using PRP penile injections for therapy of PD, showing reduction of curvature, plaque size and improvement in quality of life. Preclinical studies in rats have shown the potential benefit of adipose-derived stem cells, with improvements in erectile function and fibrosis. Human studies with mesenchymal stem cells demonstrated promising results, with reduction of curvature and plaque size. ESWL effects on PD were investigated in randomized clinical trials and demonstrated no significant impact in curvature or plaque size, but reasonable effect on pain control. Conclusion: Restorative therapies has emerged as an innovative treatment option for PD and the results from current studies appear to be promising and demonstrated good safety profile. Unfortunately, due to scarce evidence, PRP and SCT are still considered experimental by American Urological Association (AUA) and European Association of Urology (EAU) guidelines. ESWT is recommended, by the same guidelines, for pain control only. More high-quality studies with long-term follow-up outcomes are needed to evaluate efficacy and reproducibility of those therapies. Objective PRP, , (PRP) (SCT (ESWL (PD) guidelines Methods 200 202 Peyronie s Disease, Disease Disease" Penile curvature" Plasma, Plasma" cells" therapy. . therapy" fulltext full text included opinions Results life adiposederived adipose derived fibrosis Conclusion profile Unfortunately AUA (AUA EAU (EAU recommended only highquality high longterm long term followup follow up (PRP (PD 20 2

RESUMEN El objetivo general fue analizar el mecanismo a través del cual la academia puede contribuir al logro de los Objetivos de Desarrollo Sostenible (ODS) en Paraguay. Ante la necesidad de visibilizar esta contribución por parte de la academia, se remarca el propósito del estudio, intercambiar experiencias de cómo puede la academia colaborar al logro de los ODS, la importancia de difundir informaciones relacionadas a los ODS y, sobre todo, indagar el grado de conocimiento de los ODS de personas clave de organizaciones o instituciones de las distintas comunidades de la zona de influencia de la sede Central y las Filiales de la FCE-UNA. El estudio analítico-descriptivo, está basado esencialmente en un trabajo de campo, con relevamiento de datos definida como población objetivo a líderes comunitarios. Los resultados obtenidos permiten afirmar que la visión de los líderes sobre el rol de las universidades para el logro de los ODS en Paraguay, refieren a que su rol es fundamental para el logro de los ODS y también mencionan que su rol es ser nexo con la sociedad para el logro de los ODS. Se concluye que el mecanismo óptimo a través del cual la academia/universidad puede contribuir al logro de los ODS en Paraguay, seria preferentemente incluir el tema de los ODS en el currículo de las facultades y brindar cursos sobre los ODS; otro, además sería elaborar e implementar proyectos en línea de los ODS, al que se agregan, el asesorar a las instituciones responsables del cumplimiento de los ODS.

ABSTRACT The overarching objective was to examine the manner in which academic institutions can contribute to the attainment of the Sustainable Development Goals (SDGs) in Paraguay. In light of the necessity to make this contribution by academia visible, the objective of the study is to facilitate an exchange of experiences on how academia can collaborate to achieve the Sustainable Development Goals (SDGs), the importance of disseminating information related to the SDGs, and, most importantly, to investigate the degree of knowledge of the SDGs among key individuals from organizations or institutions in various communities within the area of influence of the Central Headquarters and the Branches of FCE-UNA. The study is primarily analytical and descriptive in nature, with a particular focus on fieldwork and the collection of data from community leaders. The findings indicate that the leaders perceive the role of universities in achieving the SDGs in Paraguay to be of paramount importance. Furthermore, they assert that their role is to serve as a conduit between society and the achievement of the SDGs. It is thus concluded that the most effective mechanism through which academia/ universities can contribute to the achievement of the SDGs in Paraguay would be to integrate the topic of the SDGs into the curricula of the faculties and to offer courses on the SDGs. Additionally, another avenue for contribution would be the development and implementation of SDG-aligned projects, in collaboration with institutions responsible for achieving the SDGs

Abstract: Introduction: Health care by nursing professionals to people with gender diversity can be negatively affected by the heteronormativity that has historically governed society. It is challenging to safeguard and seek actions to provide inclusive humanized care Objective: To understand the nursing professional’s experiences when providing humanized care to people with gender diversity. Method: Qualitative research with a phenomenological approach. Four nursing professionals were interviewed. The information was collected through semi-structured individual and group interviews; the discourses were analyzed, and central concepts were triangulated.Results: Humanized care is manifested through three dimensions: experiences in providing humanized care, factors that facilitate the delivery of humanized care and factors that hinder it. The categories included in the first dimension are equality, empathy, respect and learning. Factors that facilitate humanized care have the categories of welcoming and continuing education, and factors that hinder it have the categories of social name and suspicion. Conclusion: Humanized care for people with gender diversity requires values of equality and empathy, together with inclusive training from undergraduate level. Deficiency in the development of soft skills hinders the nurse-patient relationship, while suspicion of the gender-diverse person hinders first consultations. The integration of the social name and continuous training are essential to promote an environment of trust and reduce the feeling of vulnerability and discrimination.

Resumo: Introdução: O atendimento em saúde prestado pelos profissionais de enfermagem às pessoas com diversidade de gênero pode ser afetado negativamente pela heteronormatividade que historicamente rege a sociedade. Atualmente, é um desafio resguardar e buscar ações para proporcionar um cuidado humanizado e inclusivo. Objetivo: Compreender as vivências do profissional de enfermagem ao prestar o cuidado humanizado a pessoas com diversidade de gênero. Método: Pesquisa qualitativa, com abordagem fenomenológica. Foram entrevistados quatro profissionais de enfermagem. As informações foram coletadas por meio de entrevistas semiestruturadas individuais e em grupo; os discursos foram analisados e foi realizada uma triangulação dos principais conceitos. Resultados: O cuidado humanizado se manifesta por meio de três dimensões: vivências ao fornecer cuidados humanizados, fatores que facilitam a prestação de cuidado humanizado e fatores que a dificultam. As categorias incluídas na primeira dimensão são igualdade, empatia, respeito e aprendizado. Os fatores que facilitam os cuidados humanizados incluem acolhimento e formação contínua, enquanto os fatores que os dificultam incluem o uso do nome social e o receio. Conclusão: O cuidado humanizado a pessoas com diversidade de gênero requer valores de igualdade e empatia, juntamente com formação inclusiva desde o nível de graduação. A deficiência no desenvolvimento de habilidades interpessoais dificulta o relacionamento enfermeiro-paciente, enquanto o receio da pessoa com diversidade de gênero dificulta as primeiras consultas. A integração do nome social e a formação continuada são essenciais para promover um ambiente de confiança e reduzir a sensação de vulnerabilidade e discriminação.

Resumen: Introducción: La atención en salud de los profesionales de enfermería a personas con diversidad de género puede verse afectada negativamente por la heteronormatividad que ha regido históricamente la sociedad. Actualmente es un desafío resguardar y buscar acciones para entregar un cuidado humanizado inclusivo. Objetivo: Comprender las vivencias del profesional de enfermería al brindar el cuidado humanizado a personas con diversidad de género. Método: Investigación cualitativa, con un enfoque fenomenológico. Se entrevistó a cuatro profesionales de enfermería. La información se recopiló a través de entrevistas individuales y grupales semiestructuradas; los discursos fueron analizados y se realizó una triangulación de los conceptos principales. Resultados: El cuidado humanizado se manifiesta a través de tres dimensiones: vivencias al brindar cuidados humanizados, factores que facilitan la entrega del cuidado humanizado y factores que lo dificultan. Las categorías incluidas en la primera dimensión son igualdad, empatía, respeto y aprendizaje. Los factores que facilitan los cuidados humanizados tienen como categorías la acogida y la formación continua, y los factores que lo dificultan tienen las categorías de nombre social y recelo. Conclusión: El cuidado humanizado a personas con diversidad de género requiere valores de igualdad y empatía, junto con una formación inclusiva desde pregrado. La deficiencia en el desarrollo de habilidades blandas dificulta la relación enfermero-paciente, mientras que el recelo de la persona con diversidad de género obstaculiza las primeras consultas. La integración del nombre social y la formación continua son esenciales para promover un ambiente de confianza y reducir la sensación de vulnerabilidad y discriminación.

Abstract: The use of e-commerce to enhance consumer relationships is becoming increasingly important in the information society. The constant development of technologies allows existing platforms to benefit from their characteristics; however, these advancements also bring various legal issues that must be addressed to protect users' interests and rights. Blockchain technology is revolutionizing B2C electronic relations due to its transnational nature, decentralization, guarantee of trust, immutability, and one of its most distinctive functionalities: smart contracts. These agreements are characterized by their automation and self-execution, features that can conflict with existing consumer and user legislation. Additionally, the opportunities provided by Blockchain, such as allowing users to act anonymously, may contradict regulations concerning the determination of international judicial jurisdiction and the applicable law for B2C consumer legal relationships. This work aims to analyze the applicability of smart contracts in e-commerce, particularly for B2C companies, and to study the potential issues they pose for current regulations, in order to determine whether these regulations need to be adapted for these technologies

Resumen: El uso del comercio electrónico para celebrar relaciones de consumo cada vez toma más protagonismo en la sociedad de la información. El constante desarrollo de las tecnologías hace que las plataformas existentes puedan beneficiarse de sus características; sin embargo, también traen consigo diferentes cuestiones jurídicas que deben mitigarse a fin de proteger los intereses y derechos de los usuarios. La tecnología blockchain es una de las tecnologías que viene a revolucionar el ámbito de las relaciones electrónicas B2C gracias a su carácter transnacional, descentralización, garantía de confianza, inmutabilidad y a una de sus funcionalidades más características: los smart contracts. Este tipo de acuerdos se distinguen por su automatización y autoejecución, caracteres que a priori pueden poner en riesgo la legislación existente sobre consumidores y usuarios. Asimismo, las oportunidades que brinda blockchain de cara a que los usuarios puedan actuar de forma irreconocible puede poner en jaque la normativa encargada de determinar la competencia judicial internacional y la ley aplicable a las relaciones jurídicas de consumo B2C. Este trabajo analiza la aplicabilidad de los smart contracts en el ámbito del comercio electrónico, en especial en el de empresas con consumidores (B2C) y estudiar los posibles inconvenientes que generan sobre la normativa existente a fin de analizar si es necesario adaptar la legislación a estas tecnologías

Resumo: A utilização do comércio eletrônico para efetivar relações de consumo assume cada vez mais um papel central na sociedade da informação. O constante desenvolvimento das tecnologias faz com que as plataformas existentes possam beneficiar-se das suas características, no entanto, também trazem consigo diversas questões jurídicas que devem ser mitigadas de forma a proteger os interesses e direitos dos usuários. A tecnologia blockchain é uma das tecnologias que veio revolucionar o campo das relações eletrônicas B2C graças ao seu carácter transnacional, descentralização, garantia de confiança, imutabilidade e uma das suas funcionalidades mais características, os smart contracts. Estes tipos de acordos distinguem-se pela sua automatização e auto-execução, características que, a priori, podem colocar em risco a legislação existente sobre consumidores e usuários. Da mesma forma, as oportunidades que o Blockchain oferece para que os usuários possam agir de forma irreconhecível podem colocar em xeque os regulamentos encarregados de determinar a competência judicial internacional e a lei aplicável às relações jurídicas de consumo B2C. O objetivo deste trabalho é analisar a aplicabilidade dos smart contracts no campo do comércio eletrônico, especialmente o de empresas com consumidores (B2C) e estudar os possíveis inconvenientes que eles geram sobre a regulamentação existente, a fim de analisar se é necessário adaptar a legislação a estas tecnologias

Abstract This article deals with the repercussions and meanings of denialism and political irresponsibility in the relationship between risks of death and protection of the population during Covid-19 between 2019 and 2022. Vaccines, masks and isolation were denied. It aims to understand and deepen the relationship between lack of protection, misinformation, fake news and mental health. The official discourse was analyzed as a case study of bionecropolitics with repercussions on the population's health. The result shows that both physical and mental health are affected by denialism, misinformation, bad information and a lack of ethics and responsibility, with repercussions on representations of reliability, sociability and the incidence of anxiety, stress and depression. Meanings of life and death are paradoxically attributed to Covid-19 and established politics. The repercussions of diseases are more evident in the poorest and most peripheral layers in a society structured by economic, racial and gender inequality with the intersectionality of these dimensions. Covid19 Covid 19 Covid-1 201 2022 Vaccines denied misinformation populations s responsibility reliability anxiety depression politics economic dimensions Covid1 1 Covid- 20 202 2

Resumo Este artigo trata das repercussões e dos sentidos do negacionismo e da irresponsabilidade política na relação entre riscos de morte e proteção da população durante a covid-19 entre 2019 e 2022. Negaram-se vacinas, máscaras e isolamento. Tem como objetivo compreender e aprofundar a relação entre a desproteção, a desinformação, as fakes news e a saúde mental. Analisou-se o discurso oficial como um estudo de caso de bionecropolítica com repercussões na saúde da população. O resultado mostra que tanto a saúde física como a mental são afetadas pelo negacionismo, pela desinformação, pela má informação e pela falta de ética e de responsabilidade com repercussões nas representações de confiabilidade, na sociabilidade e na incidência de ansiedade, estresse e depressão. Sentidos de vida e morte são paradoxalmente atribuídos à covid-19 e à política estabelecida. As repercussões de doenças estão mais evidenciadas nas camadas mais pobres e periféricas em uma sociedade estruturada pela desigualdade econômica, de raça e de gênero com interseccionalidade dessas dimensões. covid19 covid 19 covid-1 201 2022 Negaramse Negaram se vacinas isolamento desproteção desinformação Analisouse Analisou confiabilidade ansiedade depressão estabelecida econômica dimensões covid1 1 covid- 20 202 2

The concept of digital transformation (DT) has revolutionized different facets of society including institutions of learning, where it has continued to influence teaching, learning and research. Over time and with the continued technological advancements in biological science disciplines, it has become clear that the increased convergence between these disciplines and digital systems application will become more apparent. For example, the advent and application of next-generation sequencing (NGS), machine learning (ML), artificial intelligence (AI) and other automated biological processes have afforded unique huge data sets with high societal impact. As such, digitalization of the bioeconomy remains necessary. The digitalization of the biosciences has birthed emerging concepts like "digital biology", "engineered biology" and "bio-revolution" and involve computer-centered biosciences. These are built around the integration of scientific data, modeling at several levels, as well as networked science. Nonetheless, it is still plagued by information derivation policies and technicality issues. Although, digital biology has seen a major boost in recent years with the emergence of new and updated digital biology databases and platforms for advanced computational modelling, and connection and visualization of bio-data at a molecular level, more efforts are needed from governmental stakeholders (policy makers), industry and academia to enhance the impact of computational automation and digital transformation in bioscience activities. Hence, a steady upscale in digitalization of the biosciences would ensure bioprocess development, increase teaching, research efficiency and productivity, birth more innovative future applications for various industries, and thereby impact several aspects of life. This article presents how selected biological science disciplines have been digitally transformed through data analytics and how the concept could be further explored in advancing teaching, learning and research.

Abstract: The article explores the relationship between memory and technology in Bernard Stiegler's work, focusing its analysis on "tertiary retention," a form of memory externalized in technical artifacts. Technology, beyond merely storing information, reconfigures mnemotechnic processes and human identity, challenging traditional distinctions between organic and artificial memory. The digital era transforms how we remember and construct personal and collective narratives, presenting both opportunities for cognitive enrichment and risks of eroding internal memory and cultural identity. Stiegler's philosophical approach invites critical reflection on the ethical and existential implications of technology in contemporary society.

Resumen: El artículo explora la relación entre memoria y tecnología en la obra de Bernard Stiegler, centrando su análisis en la "retención terciaria", una forma de memoria externalizada en artefactos técnicos. La tecnología, más allá de almacenar información, reconfigura los procesos mnemotécnicos e identidad humana, desafiando las distinciones tradicionales entre memoria orgánica y artificial. La era digital transforma cómo recordamos y construimos narrativas personales y colectivas, presentando tanto posibilidades de enriquecimiento cognitivo, como riesgos de erosión de la memoria interna y la identidad cultural. El enfoque filosófico de Stiegler invita a una reflexión crítica sobre las implicaciones éticas y existenciales de la tecnología en la sociedad contemporánea.