Abstract Thermal resilience refers to a building’s capacity to adapt to extreme thermal variations, maintaining a healthy environment for its occupants. This study aims to assess the thermal resilience of a naturally ventilated low-income residential building in overheating conditions within a region of savannah tropical climate. Various indices, including adaptive thermal comfort, Indoor Overheating Degree (IOD) and Exceedance Hours (HE), Heat Index (HI), and Standard Effective Temperature (SET) were calculated in current and climate change scenarios using two models, a standard building (HISp) and a building that incorporates passive bioclimatic strategies (HISe).It was demonstrated that thermal insulation and low absorption strategies significantly contribute to indoor environmental quality, reducing the risk of overheating exposure in all evaluated scenarios. Despite reducing discomfort hours and critical thermal stress levels, the idealized strategies do not provide adequate habitability conditions for the occupants. Overheating will become even more severe in projected future scenarios. Among the two dwellings, HISe demonstrates a superior potential to mitigate the risk of overheating compared to HISp in all the scenarios evaluated. buildings s variations occupants lowincome income indices comfort IOD (IOD HE, HE , (HE) HI, HI (HI) SET (SET models (HISp HISe.It HISeIt .It It quality levels dwellings (HE (HI

Resumo A resiliência térmica se refere à capacidade de um edifício de se adaptar às variações térmicas extremas, mantendo um ambiente saudável para seus ocupantes. Este estudo visa avaliar a resiliência térmica de uma edificação residencial de baixa renda, naturalmente ventilada, em situação de sobreaquecimento, localizada em região climática tropical de savana. Vários índices, incluindo conforto térmico adaptativo, Grau de Sobreaquecimento Interior (IOD) e Horas Excedentes (HE), Índice de Calor (HI) e Temperatura Efetiva Padrão (SET) foram calculados, no presente e cenários futuros de mudança climática, utilizando-se dois modelos, uma edificação padrão (HISp) e uma edificação que incorpora estratégias bioclimáticas passivas (HISe). Evidenciou-se que as estratégias de isolamento térmico e baixa absorção contribuíram significativamente para a qualidade ambiental interior, reduzindo os riscos de exposição ao sobreaquecimento. Apesar de reduzir as horas de desconforto e os níveis mais críticos de estresse térmico, as estratégias idealizadas não promovem condições adequadas de habitabilidade aos seus ocupantes. O sobrequecimento se tornará ainda mais severo nos cenários futuros projetados. Entre as habitações, a HISe demonstra um potencial superior ao da HISp para mitigar o risco de sobreaquecimento em todos os cenários avaliados. extremas ocupantes renda ventilada savana índices adaptativo IOD (IOD HE, HE , (HE) HI (HI SET (SET calculados utilizandose utilizando modelos (HISp HISe. . (HISe) Evidenciouse Evidenciou interior projetados habitações avaliados (HE (HISe

Resumen (Objetivo) Tryapanosoma cruzi, un agente causal de la enfermedad de Chagas, es un parásito que presenta una alternancia en su ciclo de vida entre un hospedador invertebrado (triatomino) y uno vertebrado (mamífero). Varios estudios han demostrado que en T. cruzi, HSP83 (homólogo de HSP90) es esencial para la división celular y el control de la respuesta al estrés térmico. Esta investigación se concentró en el estudio de la clonación, la caracterización bioinformática y la expresión del gen de la proteína de choque térmico HSP83 de T. cruzi para estudios posteriores sobre señalización. (Metodología) El ARN fue extraído de epimagostigotes T. cruci (clon EPm6/MOHM/VE/2007/ 6c), utilizando un kit comercial. El ADNc que codifica HSP83 se obtuvo utilizando RT-PCR, a partir del ARNm extraído, para lo cual se diseñaron los cebadores con base en la secuencia HSP83 de la cepa T. cruzi CL Brener. La clonación se realizó usando pGEM®T-Easy y se subclonó en el vector de expresión pQE30. Se efectuó la caracterización bioinformática y de secuencias. El gen se expresó y la proteína recombinante se purificó por cromatografía de afinidad, igual que se identificó por inmunotransferencia. (Resultados) El análisis de secuencia mostró similitud con el gen HSP83 de Trypanosoma cruzi y se observaron dominios HSP, así como epítopos B en la secuencia. Después de 3 horas de inducción con IPTG, se obtuvo una proteína recombinante con un peso aproximado de 83 kDa. La reacción de inmunotransferencia con suero hiperinmune anti-epimastigote de T. cruzi permitió la detección de una sola banda con un peso molecular de aproximadamente 83 kDa. (Conclusiones) Todos los resultados indican que se logró la clonación y caracterización de HSP83 de Trypanosoma cruzi.

Resumo (Objetivo) O Tryapanosoma cruzi, agente causador da doença de Chagas, é um parasita que apresenta uma alternância em seu ciclo de vida entre um hospedeiro invertebrado triatomíneo) e um vertebrado(mamífero). Vários estudos demonstraram que, no T. cruzi, a HSP83 (homóloga da HSP90) é essencial para a divisão celular e o controle da resposta ao estresse térmico. Esta pesquisa concentrou-se no estudo da clonagem, caracterização bioinformática e expressão do gene da proteína de choque térmico HSP83 do T. cruzi para estudos adicionais de sinalização. (Metodologia) O RNA foi extraído de epimastigotas de T. cruzi (clone EPm6/MOHM/VE/2007/ 6c) usando um kit comercial. O cDNA que codifica a HSP83 foi obtido por meio de RT-PCR a partir do mRNA extraído, para o qual os primers foram projetados com base na sequência da HSP83 da cepa T. cruzi CL Brener. A clonagem foi realizada com o pGEM®T-Easy e subclonada no vetor de expressão pQE30. Foi realizada a caracterização bioinformática e de sequência. O gene foi expresso e a proteína recombinante foi purificada por cromatografia de afinidade e identificada por imunotransferência. (Resultados) A análise da sequência mostrou similaridade com o gene HSP83 do Trypanosoma cruzi e os domínios HSP, bem como os epítopos B, foram observados na sequência. Após 3 horas de indução com IPTG, foi obtida uma proteína recombinante com peso aproximado de 83 kDa. A imunotransferência com soro hiperimune antiepimastigota de T. cruzi permitiu a detecção de uma única banda com peso molecular de aproximadamente 83 kDa. (Conclusões) Todos os resultados indicam que a clonagem e a caracterização da HSP83 do Trypanosoma cruzi foram alcançadas.

Abstract (Objective) Trypanosoma cruzi, a causal agent of Chagas’ disease, is a parasite whose life cycle alternates between an invertebrate (triatomine) and a vertebrate (mammal) host. Various studies have shown that in T. cruzi, HSP83 (a homolog of HSP90) is essential for cell division and control of the response to thermal stress. This investigation focused on studying the cloning, bioinformatic characterization, and expression of the heat shock protein HSP83 T. cruzi gene for further cell signaling studies. (Methodology) RNA was extracted from T. cruzi epimastigotes (EPm6 clone, MHOM/VE/2007/ 6c) using a commercial kit. The cDNA encoding HSP83 was determined using RT-PCR on extracted mRNA, for which the primers were designed based on the HSP83 sequence of T. cruzi strain CL Brener. Cloning was performed using pGEM®T-Easy and subcloned into the expression vector pQE30. Sequence and bioinformatic characterization were performed. The gene was expressed, and the recombinant protein was purified using affinity chromatography and identified through immunoblotting. (Results) Sequence analysis showed similarity to the gene encoding HSP83 from Trypanosoma cruzi, and HSP domains and B epitopes in the sequence were also observed. After 3 hours of induction with IPTG, a recombinant protein with an approximate weight of 83 kDa was obtained. The immunoblotting reaction with hyperimmune anti-T. cruzi epimastigote serum helped detect a single band with a molecular weight of nearly 83 kDa. (Conclusions) All results indicate that the cloning and characterization of HSP83 from Trypanosoma cruzi was achieved.

ABSTRACT The production process of non-centrifuged cane sugar (NCS) involves a significant release of steam and heat due to the evaporation of cane juices in saucepans over a solid fuel oven. This results in a warm and moisture-saturated environment, which can be suffocating for workers. This study aimed to evaluate the bioclimatic behavior of an NCS processing facility, based on the wet bulb globe temperature (WBGT) index. In addition, solutions are suggested to mitigate possible adverse effects of heat stress. This evaluation utilized computational simulations to determine the thermal comfort perceived by the workers. The accuracy of simulations was verified against temperature and relative air humidity data collected from the facility. Bioclimatic simulations encompassed twelve treatments, involving modifications to the enclosure on the walls and lantern window, incorporating three types of roof material. The WBGT index was determined by considering the effects of the radiant heat generated by the oven and the natural ventilation area on the facility’s temperature and relative air humidity. This helps to assess the comfort experienced by the workers. The thermal zone of the oven presented heat stress conditions; therefore, rest periods and mechanical ventilation were suggested when dissipating heat and steam through natural ventilation is not possible. For workers exposed to high temperatures and thermal radiation, the use of an aluminized apron and infrared goggles for eye protection was recommended. noncentrifuged non centrifuged (NCS moisturesaturated moisture saturated environment facility (WBGT addition treatments window material facilitys s conditions therefore radiation recommended

RESUMO O processo de produção de rapadura gera alta concentração de vapor e calor devido à evaporação do caldo da cana em panelas sobre forno a combustível sólido. Isso gera um ambiente quente e saturado de umidade que pode ser sufocante para os trabalhadores. Este estudo teve como objetivo avaliar as condições ambientais de uma instalação de processamento de rapadura, com base no Índice de Bulbo Úmido e Temperatura de Globo (IBUTG) e sugerir soluções para mitigar possíveis efeitos adversos do estresse térmico. Esta avaliação utilizou simulação computacional para determinar o conforto térmico dos trabalhadores. A simulação foi validada com dados de temperatura e umidade relativa do ar da instalação. As simulações bioclimáticas foram realizadas em doze tratamentos, modificando o fechamento nas paredes e lanternim, com três tipos de materiais de cobertura. Foi determinado o Índice IBUTG, considerando os efeitos do calor radiante gerado pelo forno, e pela área de ventilação natural sobre a temperatura e umidade relativa do ar no interior da instalação, e seu efeito no conforto dos trabalhadores. A zona térmica do forno apresentou condições de estresse térmico, portanto, foram sugeridos períodos de descanso e ventilação mecânica quando não for possível a evacuação do calor e vapor através da ventilação natural. O uso de avental aluminizado e óculos infravermelhos para proteção ocular foi recomendado para os trabalhadores expostos a altas temperaturas e radiação térmica. sólido IBUTG (IBUTG tratamentos lanternim cobertura portanto

ABSTRACT Heat and drought stress, which often co-occur due to water evaporation, are two major abiotic factors limiting the production of rice (Oryza sativa L.) It is crucial to enhance understanding of the effects of these abiotic stresses in rice, particularly for rice-producing countries like Malaysia, which has yet to achieve rice self-sufficiency. This greenhouse study was conducted to evaluate the morphological changes of two important Malaysian cultivars (‘MR219’ and ‘MR303’) at vegetative, reproductive, and ripening stages, as well as their physiological response and yield components under normal (control), heat, drought, and combined heat-drought stress conditions. Individual heat stress greatly influenced rice growth and yield, with significant differences (p < 0.01) observed across all examined parameters except the grain to leaf area index ratio (GtoLAI). Conversely, individual drought stress mostly affected yield-related parameters, with significant differences (p < 0.01) in grain weight (GW), harvest index (HI), and percentage of filled grain (%FG). Interestingly, the combined stresses in this study did not significantly affect plant height (PH) for all growth stages and most yield-related traits (HI, GW, and GtoLAI). The majority of the significant changes (p < 0.01) were observed on physiological traits, including chlorophyll a (Chl A) and b (Chl B). We found a positive correlation between HI and %FG (R2 = 0.3974**) under heat and drought stress, indicating that improving either of these traits can boost rice production. Collectively, our study revealed that the individual effects of heat and drought on rice growth and yield can differ from the effects of combined stress.

Resumo Nas últimas décadas, uma epidemia de doença renal crônica (DRC) tem sido associada a fatores ambientais e ocupacionais (estresse térmico decorrente de cargas de trabalho elevadas em altas temperaturas e exposição a produtos químicos, como agrotóxicos e metais), denominada DRC de origem não tradicional (DRCnt). Esta revisão descritiva tem como objetivo apresentar evidências recentes sobre estresse térmico, agrotóxicos e metais como possíveis causas de DRCnt e fornecer uma visão geral das estratégias brasileiras de regulamentação, fiscalização e vigilância sanitária relacionadas. Os trabalhadores brasileiros são comumente expostos a condições extremas de calor e outros fatores de risco de DRCnt, incluindo o aumento da exposição a agrotóxicos e metais. Além disso, há uma falta de regulamentação e fiscalização, políticas públicas e estratégias adequadas para proteger a saúde renal dos trabalhadores em relação aos principais fatores de risco. É provável que a DRCnt seja uma causa significativa de DRC no Brasil, uma vez que a etiologia da doença é desconhecida em muitos pacientes e diversas condições para seu desenvolvimento estão presentes no país. Estudos epidemiológicos devem ser realizados para explorar associações causais e estimar o impacto do calor, dos agrotóxicos e dos metais na DRCnt no Brasil. Além disso, as políticas públicas devem priorizar a redução da exposição dos trabalhadores e a promoção de sua saúde e segurança. décadas (DRC químicos metais, , metais) DRCnt. . (DRCnt) relacionadas disso Brasil país segurança (DRCnt

Abstract In the past decades, an epidemic of chronic kidney disease (CKD) has been associated with environmental and occupational factors (heat stress from high workloads in hot temperatures and exposure to chemicals, such as pesticides and metals), which has been termed CKD of non-traditional origin (CKDnt). This descriptive review aims to present recent evidence about heat stress, pesticides, and metals as possible causes of CKDnt and provide an overview of the related Brazilian regulation, enforcement, and health surveillance strategies. Brazilian workers are commonly exposed to extreme heat conditions and other CKDnt risk factors, including increasing exposure to pesticides and metals. Furthermore, there is a lack of adequate regulation (and enforcement), public policies, and strategies to protect the kidney health of workers, considering the main risk factors. CKDnt is likely to be a significant cause of CKD in Brazil, since CKD’s etiology is unknown in many patients and several conditions for its development are present in the country. Further epidemiological studies may be conducted to explore causal associations and estimate the impact of heat, pesticides, and metals on CKDnt in Brazil. Moreover, public policies should prioritize reducing workers´ exposure and promoting their health and safety. decades (CKD chemicals metals, , metals) nontraditional non traditional CKDnt. . (CKDnt) enforcement Furthermore enforcement) Brazil CKDs s country Moreover safety (CKDnt

Abstract In areas with arid and semi-arid climates, the availability and quality of water are critical factors for the development of agronomy. To face the challenge, an alternative for the use of this resource is the implementation of efficient and sustainable techniques such as the use of drip irrigation. The objective of the work was to analyze the agronomic behavior of rye under unfavorable water and temperature conditions to determine the effectiveness of phenotypic stability on La Costa de Hermosillo, Sonora, Mexico. Two rye genotypes were planted under a split plot experimental design (large plot: three water levels and small plot: two genotypes of seeds obtained in different cycles). The agronomic characteristics of the plant, the physicochemical characteristics of the grain were evaluated; and the significance between the different variables was determined with a correlation analysis. The analysis of variance revealed effects on plant height, number of spikes and grains, grain size, thousand grain weight, yield and protein content of rye due to the different water regimes used. Between 219-314 spikes per linear meter were obtained, with a commercially acceptable grain size (7.45-7.95 mm long x 2.18-2.40 mm wide). High yield values (1.72-3.11 t ha-1) were also observed, as well as excellent protein content (20.3-22.8%). These results demonstrate that rye is a versatile cereal that adapts to adverse environmental conditions such as a decrease in the amount of irrigation water and extremely high temperatures.

Resumen En zonas de clima árido y semiárido, la disponibilidad y la calidad del agua son factores críticos para el desarrollo de la agronomía. Para enfrentar el desafío, una alternativa para el aprovechamiento de este recurso es la implementación de técnicas eficientes y sostenibles como el uso de riego por goteo. El objetivo del trabajo fue analizar el comportamiento agronómico del centeno en condiciones desfavorables de agua y temperatura para determinar la efectividad de la estabilidad fenotípica en la Costa de Hermosillo, Sonora, México. Se sembraron dos genotipos de centeno bajo un diseño experimental de parcelas divididas (parcela grande: tres niveles de agua y parcela chica: dos genotipos de semillas obtenidas en ciclos diferentes). Se evaluaron las características agronómicas de la planta, las características fisicoquímicas del grano; y se determinó la significancia entre las distintas variables con un análisis de correlación. El análisis de varianza reveló efectos sobre la altura de la planta, el número de espigas y de granos, tamaño de grano, el peso de mil granos, rendimiento y contenido de proteína del centeno debido a los diferentes regímenes hídricos utilizados. Se obtuvieron entre 219-314 espigas por metro lineal, con tamaño de grano comercialmente aceptable (7.45-7.95 mm largo x 2.18-2.40 mm ancho). También se observaron valores de rendimiento altos (1.72-3.11 t ha-1), así como un excelente contenido de proteína (20.3-22.8%). Estos resultados demuestran que el centeno es un cereal versátil que se adapta a condiciones ambientales adversas como disminución en la cantidad de agua de riego y temperaturas extremadamente altas.

SUMMARY Introduction: The food sector includes a reasonable number of workers involved. Empirically, there is no doubt that this sector has work areas that by their nature involve a series of risk factors that can affect the health. Knowing and cataloguing these, in order to be able to draw up a preventive plan, is essential for the health of the worker (and the entity that employs him). This article aims to gather the main occupational risk factors/occupational risks, occupational diseases and recommended protective measures at kitchen workers. Methodology: This is a Scoping Review, initiated through a search carried out in the Medline database (via Pubmed), carried out during the month of November 2023, encompassing articles (in Portuguese and English), with the keywords "kitchen worker" and "occupational" in the title, with no time limit. The risk analysis tool "Online interactive risk assessment" of the European Agency for Safety and Health at Work was used as a complement. Content: The main risk factors found are exposure to chemical agents (products used to clean facilities and/or fruit and vegetables, or by-products from cooking); ergonomic risks (maintained/forced postures; repetitive movements, manual handling of loads); accidents (falls at the same level or at different levels; handling of equipment/utensils that increase the risk of burns, cuts and/or amputations); contact with electrical equipment, noise, heat stress and psychosocial risks. The main occupational diseases refer to musculoskeletal, psychosocial or dermatological conditions. Each unit should, however, be evaluated individually, according to its intrinsic characteristics. Conclusions: The safety and health of kitchen workers are issues that deserve constant attention from employers, regulators and society at large. This article explored some of the main occupational hazards and listed the need for effective preventive measures. Reducing these risks is not only an ethical and legal responsibility, but a smart strategy to improve operational efficiency and quality of services, avoiding accidents, minimizing absenteeism, but also improving the productivity of companies. It would be pertinent to develop investigations that assess the national reality

RESUMO Introdução: O setor da alimentação coletiva inclui um número razoável de trabalhadores envolvidos. Empiricamente, não existem dúvidas de que este setor possui áreas de trabalho que pela sua natureza envolvem uma série de fatores de risco que podem afetar a saúde dos trabalhadores. Conhecer e catalogar os mesmos, de modo a poder elaborar um plano preventivo, é essencial para a saúde do trabalhador (e da entidade que o emprega). Este artigo objetiva reunir os principais fatores de risco/riscos ocupacionais, doenças profissionais associadas e medidas de proteção recomendadas. Metodologia: Trata-se de umaScoping Review, iniciada através de uma pesquisa realizada nas base de dados Medline (via Pubmed), realizada durante o mês de novembro de 2023, englobando artigos (em português e inglês), com as palavras-chave “kitchen worker” e “occupational” no título, sem limite temporal. Utilizou-se como complemento a ferramenta de análise de riscos “Online interactive risk assessment”, da Agência Europeia para a Segurança e Saúde no Trabalho. Conteúdo: Os principais fatores de risco encontrados são exposição a agentes químicos (produtos na higienização das instalações e/ou hortofrutícolas, ou subprodutos derivados da confeção); riscos ergonómicos (posturas mantidas/ forçadas; movimentos repetitivos, manuseamento manual de cargas); riscos de acidentes (quedas ao mesmo nível ou a níveis diferentes; manuseamento de equipamentos/utensílios que aumentem o risco de queimaduras, cortes e/ou amputações); contato com equipamentos elétricos, ruído, stress térmico e riscos psicossociais. As principais doenças profissionais referem-se a condições musculoesqueléticas, psicossociais ou dermatológicas. Cada unidade deverá, no entanto, ser avaliada individualmente, mediante as suas caraterísticas intrínsecas. Conclusões: A segurança e saúde dos trabalhadores das unidades de alimentação coletiva são questões que merecem atenção constante dos Empregadores, reguladores e da sociedade em geral. Este artigo explorou alguns dos principais riscos ocupacionais e listou a necessidade de medidas preventivas eficazes. A redução destes riscos não é apenas uma responsabilidade ética e legal, mas uma estratégia inteligente para melhorar a eficiência operacional e a qualidade dos serviços, evitando acidentes, minimizando o absentismo, mas também melhorando a produtividade das empresas. Seria pertinente desenvolver investigações que avaliassem a realidade nacional.

Abstract L-ascorbic acid is a natural antioxidant produced by plants and their fruits. The abundance of this acid during the development and postharvest ripening of mangoes (Mangifera indica L.) depends on genetic and climatic factors. The mangoes undergo a quarantine hydrothermal treatment before being exported to control the fruit fly. A quarantine hydrothermal treatment consists of immersing mangoes in water at 46.1 °C (118 °F) for 60 to 120 min depending on the size, affecting the L-ascorbic acid content and other quality parameters of the fruit. This research aimed to evaluate the effect of QHT on L-ascorbic acid content, firmness, color, and expression of L-ascorbic acid biosynthesis and recycling genes during mango postharvest. ‘Ataulfo’ mangoes were harvested at physiological ripeness in Escuinapa, Sinaloa in 2019, subjected to QHT (46.1 °C, 75 min), hydrocooled (25 °C, 30 min) and stored at 20 °C for nine days. Mangoes subjected to QHT showed a reduction in L-ascorbic acid content (p≤ 0.05). The firmness of the pulp behaved similarly in fruits with QHT and in control fruits, while the external color of the fruits with a quarantine hydrothermal treatment indicated an advanced ripening process compared to the control fruits (p≤ 0.05). The levels of MiGME1, MiGME2, MiGGP2, and MiMDHAR transcripts increased in response to treatment, suggesting activation of the synthesis and recycling pathway to counteract the abiotic stress caused by the heat to which the mangoes were subjected.

Resumen El ácido L-ascórbico, es un antioxidante natural producido por las plantas y sus frutos. La abundancia de este ácido durante el desarrollo y la maduración poscosecha del mango (Mangifera indica L.) depende de factores genéticos y climáticos. Los mangos se someten a un tratamiento hidrotérmico cuarentenario, antes de su exportación para control de la mosca de la fruta. El un tratamiento hidrotérmico cuarentenario, consiste en sumergir los mangos en agua a 46.1 °C (118 °F) durante 60 a 120 min dependiendo del tamaño, afectando el contenido de ácido L-ascórbico y otros parámetros de calidad del fruto. El objetivo de esta investigación fue evaluar el efecto del THC en el contenido de ácido L-ascórbico, la firmeza, el color y la expresión de genes de biosíntesis y reciclaje de ácido L-ascórbico durante la postcosecha del mango. Los mangos ‘Ataulfo’ se cosecharon en madurez fisiológica en Escuinapa, Sinaloa en el año 2019, se sometieron a THC (46.1 °C, 75 min), hidroenfriado (25 °C, 30 min) y se almacenaron a 20 °C por nueve días. Los mangos sometidos al THC mostraron reducción en el contenido de ácido L-ascórbico (p≤ 0.05). La firmeza de la pulpa se comportó similar en frutos con THC y en frutos testigo, mientras que el color externo de los frutos con un tratamiento hidrotérmico cuarentenario e indicó un proceso de maduración adelantado respecto a los frutos testigo (p≤ 0.05). Los niveles de transcritos MiGME1, MiGME2, MiGGP2 y MiMDHAR aumentaron en respuesta al tratamiento, lo que sugiere la activación de la ruta de síntesis y reciclaje para contrarrestar el estrés abiótico ocasionado por el calor al que se sometieron los mangos.

Resumen El grado de calidad de piezas fabricadas por inyección de termoplásticos se puede establecer por el peso del producto, apariencia y mínimos defectos. Adicionalmente, las condiciones del proceso de inyección pueden inducir un efecto en el desempeño mecánico de las piezas inyectadas, donde los esfuerzos residuales de la pieza inyectada generados en el procesamiento pueden promover la formación de grietas o fallas prematuras del producto durante su uso al aplicarle una carga o fuerza externa. El propósito del presente trabajo es evaluar el efecto que genera el procesamiento por inyección en una pieza inyectada en el desempeño mecánico de esta. Para tal fin, se inyectaron probetas bajo diferentes condiciones de proceso y posteriormente estas fueron sometidas a pruebas mecánicas de flexión, algunas piezas fueron llevadas a un tratamiento térmico de recocido “annealing”, con el fin de obtener una relajación molecular que permita reducir sus esfuerzos internos o residuales generados durante el proceso de inyección. Adicionalmente, se hizo una evaluación cualitativa de la distribución de esfuerzos residuales en las piezas inyectadas mediante la técnica de fotoelasticidad, para complementar y validar los resultados de las pruebas mecánicas. Se halló en los resultados de las muestras inyectadas a temperatura de molde de 50 ºC una mayor liberación de esfuerzos residuales de acuerdo con las imágenes de fotoelasticidad analizadas y un mayor porcentaje de incremento en la resistencia a la flexión de estas piezas al aplicar el tratamiento térmico. Lo que podría sugerir una mayor generación de esfuerzos residuales a esta temperatura de molde con respecto a la temperatura de molde recomendada de 80 ºC. El efecto de tratamiento térmico mejora significativamente el desempeño mecánico de todas las piezas evaluadas a las diferentes condiciones de proceso.

Abstract The quality of parts manufactured by thermoplastics injection molding can be evaluated based on product weight, appearance, and presence of defects. Furthermore, the conditions of the injection process can significantly influence the mechanical performance of the molded parts. Residual stresses generated during plastic processing may contribute to the formation of cracks or premature failures when the product is subjected to external loads or forces. This study aims to evaluate the effects of the injection process on an injected part and how they affect its mechanical performance. For this purpose, specimens were injected under different process conditions and, subsequently, employed in a. Some parts were also subjected to annealing (a thermal treatment) to induce molecular relaxation, which helps to reduce internal or residual stresses generated during the injection process. Additionally, a qualitative evaluation of the distribution of residual stresses in the injected parts was conducted using the s technique to complement and validate the results of the mechanical tests. The results of the specimens injected at a mold temperature of 50°C showed a greater release of residual stresses, as indicated in photoelasticity images analyzed here, and a noticeable increase in flexural strength if the heat treatment had been applied. This suggests that more residual stresses are produced at 50°C than at the recommended mold temperature of 80°C. The heat treatment significantly improved the mechanical performance of all the parts injected in this study under different processing conditions.

Abstract Due to its allelopathic capability, the investigation of essential oils (EO) as sustainable alternatives for weed control has gained considerable interest in the last years. In this study, we investigated the phytotoxic capacity of the essential oil extracted from Carlina acaulis L. (Apiaceae) on the weed Bidens pilosa L. This essential oil, mainly composed of carlina oxide (98%), has demonstrated remarkable phytotoxic effects on the metabolism of Bidens pilosa. These effects include leaf necrosis, a reduction in relative water content and total leaf area, and an increase in the dry weight/fresh weight ratio, indicating possible alteration in the plant's water status. After EO short-exposition, analysis of the photosynthetic system revealed a significant decrease in the photochemical efficiency of photosystem II (Φ II) and the maximum photochemical efficiency of photosystem II (F v /F m ). In contrast, the energy dissipated as heat (Φ NPQ) increased significantly. Damage to photosystem II was accompanied by a decrease in manganese and calcium concentrations, suggesting an alteration in the Mn4Ca cluster of photosystem II. Metabolomics analysis revealed a general decrease in sugar content and an accumulation of isoleucine and valine, commonly associated with osmotic stress situations. Our results indicate that the EO of C. acaulis is a promising natural product with strong phytotoxic potential against Bidens pilosa, and the exploration of its effects on other weeds is suggested.

Resumen Gracias a su capacidad alelopática, la investigación de aceites esenciales (AE) como alternativas sostenibles para el control de malas hierbas ha despertado un considerable interés a lo largo de los últimos años. En este estudio, se ha evaluado la capacidad fitotóxica del AE extraído de Carlina acaulis L. (Apiaceae) en la mala hierba Bidens pilosa L. Este aceite, principalmente compuesto de óxido de carlina (98%), ha exhibido notables efectos fitotóxicos en el metabolismo de Bidens pilosa. Estos efectos incluyen necrosis en las hojas, reducción en el contenido relativo de agua y área foliar total, y un aumento en la relación peso seco/peso fresco, indicando una posible alteración en el estado hídrico de la planta. El análisis del sistema fotosintético reveló una significativa disminución en la eficiencia fotoquímica del fotosistema II (Φ II) y en la eficiencia cuántica máxima del fotosistema II (F v /F m ) tras la aplicación del AE. En contraste, la energía disipada en forma de calor (Φ NPQ) aumentó significativamente. El daño al fotosistema II coincidió con una disminución en las concentraciones de manganeso y calcio, sugiriendo una alteración en el clúster Mn4Ca del fotosistema II. El análisis metabolómico reveló acumulación de isoleucina y valina, asociadas comúnmente con estrés osmótico, y una disminución general en el contenido de azúcares. Nuestros resultados indican que el AE de C. acaulis es un producto natural prometedor con un fuerte potencial fitotóxico contra Bidens pilosa, y se sugiere la exploración de sus efectos en otras malas hierbas.

ABSTRACT The goal of this investigation was to study the influence of different values of Temperature-Humidity index (THI) on the duration of lying down and feed consumption, productivity, and heat production by dairy cows in two variants of feedlots (with and without shelters). The study was conducted from February 2007 to early August 2021 in the central part of Ukraine (Kyiv region) in different periods of THI. This index was divided into three categories: 1) 66-71: normal (14 days); 2) 72-79: alert (11 days); 3) ≥ 80: dangerous (9 days). Two variants of feedlots were selected: Open feedlot with shelters and open feedlot without shelters. The largest increase in values of THI affecting the cows was observed in open feedlots without shelters. There, in alert and danger periods duration of feed consumption decreased by 8.0 and 23.1 min, lying down by 17 and 38 min, productivity by 2.3 kg (or 8.77%) and 3.6 kg (or 13.74%), energy consumption by 4.7 and 9.6 MJ, respectively, in comparison to normal period.

RESUMEN El objetivo del estudio fue evaluar la influencia de diferentes valores de Índice temperatura-humedad (IHT) sobre el tiempo de reposo y el consumo de alimento, la productividad y la producción de calor de las vacas lecheras en dos variantes de feedlots (con y sin refugio). El estudio se llevó a cabo desde febrero de 2007 hasta principios de agosto de 2021 en la parte central de Ucrania (región de Kiev) en diferentes períodos de IHT. El índice se dividió en tres categorías: 1) 66-71: normal (14 días); 2) 72-79: alerta (11 días); 3) >80: peligroso (9 días). Se seleccionaron dos variantes de feedlots: abierto con sombra y abierto sin sombra. El mayor aumento en los valores de IHT que afectaron a las vacas se observó en los corrales de engorda abiertos sin sombras. Allí, en periodos de alerta y peligro, la duración del consumo de alimento disminuyó en 8.0 y 23.1 min, acostado en 17 y 38 min, la productividad en 2.3 kg (o 8.77%) y 3.6 kg (o 13.74%), el consumo de energía en 4.7 y 9.6 MJ, respectivamente, en comparación con el periodo normal.

An article in the July/August 2022 issue (Havenga et al., S Afr J Sci. 2022;118(7/8), Art. #13118) argued that changing the date of the Comrades Marathon from May to August would result in increased heat stress for participants. Heat stress was estimated using the Universal Thermal Climate Index (UTCI), which is designed to represent a person walking, not running. In this Commentary, I argue that using the UTCI may lead to an underestimation of heat stress for the Comrades Marathon, and that the conclusion that August has worse heat stress than May depends on the assumptions in the estimation of heat stress

We value comments on our research paper in a Commentary in this issue (Simpson, S Afr J Sci. 2024;120(1/2), Art. #16445). Acknowledging the Universal Thermal Comfort Index (UTCI)'s limitations in capturing individual physiological responses remains important; however, we argue for its appropriateness based on recent thermophysiology and heat exchange advancements during its development and broader alignment with standardised indexing efforts. Our original research paper set out with these considerations in mind, and our conclusions remain valid. We further argue for refinement of the UTCI for specific activities instead of using the RET. Finally, future efforts should focus on monitoring data in real-world scenarios to validate and improve thermal indices

ABSTRACT In 2007 the Nordic group came to the following unanimous conclusions: In general, hormonal treatment is not recommended, considering the poor immediate results and the possible long-term adverse effects on spermatogenesis. Thus, surgery is to be preferred. However, defective mini puberty inducing insufficient gonadotropin secretion is one of the most common causes of nonobstructive azoospermia in men suffering from congenital isolated unilateral or bilateral cryptorchidism. The extent of alteration in the unilateral undescended testis correlate with the contralateral descended testis, indicating that unilateral cryptorchidism is a bilateral disease. Idiopathic central hypogonadism explains the phenomenon of defective mini puberty in otherwise healthy cryptorchid boys. We therefore recommend hormonal treatment for cryptorchid boys with defective mini puberty. Gonadotropin releasing hormone agonist (GnRHa) treatment following surgery to correct cryptorchidism restores mini puberty via endocrinological and transcriptional effects and prevents adult infertility in most cases. Several genes are important for central hypogonadotropic hypogonadism in mammals, including many that are transcribed in both the brain and testis. At the molecular level, there is no convincing evidence that heat shock is responsible for the observed pathological testicular changes. Thus, impaired transformation of gonocytes is not the result of temperature stress but rather a hormonal imbalance. Cryptorchidism should therefore be considered a serious andrological problem that cannot be successfully treated by early orchidopexy alone. 200 conclusions general recommended longterm long term spermatogenesis Thus preferred However disease GnRHa (GnRHa cases mammals level changes imbalance alone 20 2

Abstract Potato plants are often subjected to heat stress during growth and development, resulting in loss of yield and quality. However, the possibility exists that commercially important genotypes show tolerance to heat stress. In this study, we aim to investigate the morphological and physiological effects of moderate conditions of heat stress on tuberization in nine potato genotypes, and how it affects propagation using an ex vitro and in vitro system. The plant material came from the In Vitro Germplasm Bank of the International Potato Center (CIP). The incubation temperatures of the cultures in in vitro and ex vitro conditions were the ambient in the laboratory and greenhouse, respectively. In in vitro system a randomized complete block design was employed in nine genotypes, two treatments: multiplication culture medium and the same culture medium supplemented with 8.0% sucrose and 500 mg/L-1 CCC, six flasks and five explants per experimental unit. Among all genotypes evaluated, Leona and Liberteña did not form tubers under ex vitro treatment conditions. Under in vitro conditions, Amarilis, Capiro, Perricholi and Tacna showed no evidence of tuberization, and Canchán was the only genotype where 100% formation of tuberous structures were produced in all evaluations. The findings concluded in this study showed that of all the potatoes evaluated, Canchán best responded to the evaluations under both in ex vitro and in vitro conditions, at maximum temperatures between 22.4 to 26.9 and 27.0 to 28.2 oC, respectively. Therefore, it is vitally important to consider its development potential in different producing locations around the world, which are directly and indirectly affected by climate change.