The rapid and expansive integration of Internet of Things (IoT) environments across various industrial sectors has led to an unprecedented surge in data generation and management. This exponential growth in data underscores the critical necessity for robust data security methodologies that can effectively safeguard the confidentiality and integrity of information without imposing undue computational burdens. In response to this challenge, numerous studies have sought to leverage Attribute-Based Encryption (ABE) as a means to enable fine-grained access control. Among the ABE variants, Ciphertext Policy ABE (CP-ABE) and bilinear pairings have emerged as popular choices to construct security schemes that strike a balance between robust protection and computational efficiency. Despite the advancements achieved through CP-ABE and bilinear pairings, a prevalent concern arises in the utilization of Linear Secret Sharing Scheme (LSSS) access policies. LSSS policies, while providing a flexible and expressive way to define access controls, can significantly impact the execution time of encryption methods. This study recognizes the importance of addressing this challenge and explores the potential of employing a Key Policy Attribute-Based Encryption (KP-ABE) approach. The primary objective is to mitigate the computational overhead associated with encryption methods, thereby enhancing the efficiency of data security measures within IoT environments. Furthermore, this research delves into the incorporation of Elliptic Curve Cryptography (ECC) to generate cryptographic keys. ECC, known for its strong security properties and computational efficiency, is considered a promising approach to bolster data security while concurrently minimizing computational overhead. By integrating KP-ABE with ECC, this study aims to offer a comprehensive solution that ensures robust security measures within the intricate landscape of IoT environments. Through detailed analysis and empirical investigation, the research endeavors to contribute valuable insights to the ongoing discourse on securing IoT data in a manner that aligns with the dual imperatives of security and computational efficiency. OPEN LICENSE: CC BY-NC-ND

Resumen Las células troncales dentales (CTDs) son células multipotentes con gran capacidad de proliferación y diferenciación multilinaje. Pocos estudios han comparado las características celulares y el potencial de diferenciación adipogénica de las CTDs derivadas de tejidos de un mismo individuo. El objetivo de este trabajo fue evaluar las diferencias en las características de crecimiento, la expresión de marcadores específicos de celulas troncales mesenquimales (CTMs) y el perfil de proteínas en respuesta a la diferenciación adipogénica, de células de pulpa dental y ligamento periodontal obtenidas de un mismo donante. Las células dentales se aislaron a partir del tercer molar de un único donante mediante el método de explante. Para obtener la curva de proliferación de las células se evaluó mediante análisis con azul tripano. Tras cultivar las células en medio adipogénico, se controlaron los cambios morfológicos mediante tinción con rojo O oleoso, así como los marcadores adipogénicos PPARγ y adiponectina mediante RT-qPCR. Por último, se realizó una electroforesis bidimensional de las proteínas aisladas de estas células para analizar el perfil proteómico. Los dos tipos de CTDs comparten características celulares similares; sin embargo, su capacidad de diferenciación adipogénica es diferente. Basándonos en los resultados del perfil proteico, identificamos cinco proteínas expresadas diferencialmente entre ambos tipos de células troncales. Los resultados mostraron que las células troncales de la pulpa dental y del ligamento periodontal de un mismo donante tienen características celulares similares pero una respuesta diferente a la adipogénesis, lo que explicaría las diferencias en la expresión de sus proteinas.

Abstract Dental stem cells (DSCs) are multipotent cells with high proliferation capacity and multilineage differentiation. Few studies have compared the cellular characteristics and adipogenic differentiation potential of DSCs derived from tissues of the same individual. The objective of this work was to evaluate the differences in growth characteristics, expression of mesenchymal stem cells (MSCs)-specific markers, and the proteomic profile in response to adipogenic differentiation, of dental pulp and periodontal ligament cells obtained from a single donor. Dental cells were isolated from the third molar of a single donor using the outgrowth method. To obtain the proliferation curve of the cells was evaluated by trypan blue analysis. After the cells were cultured in adipogenic medium, morphological changes were monitored by oil red O staining, as well as adipogenic markers PPARγ and adiponectin by RT-qPCR. Finally, a two-dimensional electrophoresis of the proteins isolated from these cells was performed to analyze the proteomic profile. The two types of DSCs share similar cellular characteristics; however, their capacity for adipogenic differentiation is different. Based on the protein profiling results, we identified five differentially expressed proteins between both types of stem cells. Our findings showed that dental pulp and periodontal ligament stem cells from a single donor have similar cellular characteristics but a different response to adipogenesis, which would explain the differences in the expression of their proteins.

Resumen Objetivo: Describir el crecimiento femoral proximal en una población latinoamericana a través del desplazamiento femoral, el ángulo cervico-diafisario, la longitud del cuello femoral y el diámetro de la cabeza femoral evaluados con tomografía computarizada en pacientes pediátricos sin enfermedad de cadera. Materiales y Métodos: Estudio retrospectivo que evaluó imágenes de tomografía computarizada de caderas de pacientes sanos <18 años. Se tomaron las siguientes medidas: desplazamiento femoral, longitud del cuello femoral, diámetro de la cabeza femoral y ángulo cervico-diafisario. Resultados: El desplazamiento femoral aumenta 1,96 mm hasta los 12.5 años, y desde los 12.5 hasta los 16 años, aumenta 1,2 mm. Se constató un aumento lineal del crecimiento de la longitud del cuello femoral. El ángulo cervico-diafisario disminuyó progresivamente hasta los 12 años. A partir de ese momento, la curva se aplanó. Se observó un aumento anual del diámetro de la cabeza femoral de 1,56 mm hasta los 13 años y de 0,62 mm anuales, en adelante. Conclusiones: Las medidas descritas en este estudio son esenciales para el seguimiento, el diagnóstico o el abordaje conductual en múltiples cuadros articulares de cadera durante el crecimiento. Se expone la necesidad de realizar estudios más amplios para establecer rangos de normalidad en la población local con las herramientas tecnológicas disponibles, que fundamenten una referencia para la restauración de la anatomía en la cirugía de preservación.

Abstract Introduction: This study aims to perform a descriptive analysis of proximal femoral growth in a Latin-American population through femoral offset, neck-shaft angle, femoral neck length, and femoral head diameter evaluated through computed tomography in pediatric patients without hip pathology. Materials and Methods: Retrospective study evaluating CT images of the hips of healthy patients under 18 years. The following measurements were taken by a trained orthopedist: femoral offset, femoral neck length, femoral head diameter, and neck-shaft angle. Results: Femoral offset increases by 1.96 mm until age 12.5. From 12.5 to 16 years of age, it increases by 1.2 mm. A constant rise in the growth of the femoral neck length was found. The neck-shaft angle presented a progressive decrease until age 12. After that point, the curve flattened. An increase in femoral head diameter of 1.56 mm per year was observed until age 13 and then 0.62 mm per year. Conclusions: The measurements evaluated in this study are essential for the diagnosis, follow-up, and treatment approach in hip pathologies during growth. More extensive research is needed to define normal ranges that will serve as a baseline for anatomy restoration in hip joint preservation surgery.

RESUMEN El objetivo de este artículo es comprobar empíricamente la existencia de la curva de Kuznets ambiental entre el crecimiento económico y la temperatura promedio utilizando datos departamentales del Perú durante el periodo 2010-2020. Como resultado, se encontró que el impacto del PBI per cápita departamental es negativo sobre la temperatura promedio, lo cual sugiere que un incremento del 1% del PBI per cápita departamental disminuiría en 1.92% la temperatura promedio en las regiones del Perú. Estos resultados son contundentes para negar la existencia de la hipótesis de la curva de Kuznets ambiental. Lo cual nos sugiere que en realidad estamos frente a una curva en forma de U.

ABSTRACT The aim of this article is to empirically test the existence of the Environmental Kuznets Curve between economic growth and average temperature using departmental data from Peru during the period 2010-2020. As a result, it was found that the impact of departmental GDP per capita is negative on average temperature, suggesting that a 1% increase in departmental GDP per capita would decrease the average temperature by 1.92% in Peru's regions. These results strongly refute the existence of the Environmental Kuznets Curve hypothesis, suggesting instead that we are facing a U-shaped curve.

Resumen El objetivo de este trabajo es conocer la trayectoria de las necesidades psicológicas básicas en estudiantes secundarios de Entre Ríos, Argentina, en tres momentos de su recorrido escolar. Para ello se realizó un diseño cuantitativo, siendo un estudio de tipo longitudinal. El muestreo utilizado fue no probabilístico intencional. La muestra se conformó por adolescentes escolarizados de Entre Ríos. Esta consistió en 313 sujetos para el primer tiempo, 280 para el segundo y 249 para el tercero. Las edades abarcaron desde los 12 hasta los 17 años. Respecto al instrumento, se utilizó la adaptación al contexto educativo de la Basic Psychological Needs Scale in General. Se realizaron análisis de modelos de curva de crecimiento latente; los hallazgos indican que la trayectoria de la autonomía puede ser explicada a través de un modelo de cambio cuadrático, la de la competencia mediante un modelo lineal y la de la relación de un modelo cuadrático.

Abstract The objective of the study is to know the trajectory of basic psychological needs in high school students from Entre Ríos, Argentina, in three moments of their school career. The design is quantitative and longitudinal. The sampling was intentional non-probabilistic. The sample consisted of adolescent high school students from Entre Ríos. It included 313 participants at the first measurement point, 280 at the second and 249 at the third. The age ranged from 12 to 17 years. The instrument used was the Basic Psychological Needs Scale in General, in a version adapted to the educational context. Date were analyzed with Latent Growth Curve Models; the findings indicate that the trajectory of autonomy can be explained through a quadratic change model, that of competence through a linear model, and that of relatedness through a quadratic model.

Resumo O objetivo deste estudo é determinar a trajetória das necessidades psicológicas básicas de estudantes da educação secundária em Entre Ríos, Argentina, em três momentos de sua carreira escolar. O desenho é quantitativo e trata-se de um estudo longitudinal. A amostragem utilizada foi não probabilística e intencional. A amostra foi composta de adolescentes de um colégio em Entre Ríos. A amostra consistiu em 313 sujeitos para o primeiro período, 280 para o segundo e 249 para o terceiro. As idades variaram de 12 a 17 anos. Com relação ao instrumento, foi utilizada a adaptação ao contexto educacional da Basic Psychological Needs Scale in General. Foram realizadas análises de modelagens de curva de crescimento latente; os resultados indicam que a trajetória da autonomia pode ser explicada com base em um modelo de mudança quadrática; a de competência, mediante um modelo linear, e a de relação, por um modelo quadrático.

SUMMARY One of the most important functions of forests is their capacity to store carbon, which varies according to floristic composition, age, and tree density. To provide information that is useful for the carbon inventories, the construction of growth curves for dominant strata within the study area was set as the objective of this work. With data from the National Forest and Soil Inventory 2005-2009 were calculated the IVI by conglomerate and from a dendrogram performed with cluster analysis, using the Ward´s linkage method, dominant strata were formed. A growth curve per community was constructed based on the maximum yields per hectare recorded in the forestry management plans, the percentage increases were attributed to volume per species at different ages, which was obtained by ICA through trunk analysis. Five dominant strata were formed; Pinus oocarpa (21.08 % to 54.38 %), Pinus douglasiana (41.45 % to 89.83 %), Pinus devoniana (27.53 % to 63.88 %), Pinus leiophylla (27.53 % to 89.03 %) and Quercus spp. (36.05 % to 93.26 %). The maximum yields for the stands within the study area were 329.98 m3 ha-1, 330.71 m3 ha-1, 171.35 m3 ha-1, 185.22 % and 127.46 m3 ha-1, respectively. The Pinus oocarpa and Pinus douglasiana dominant strata are had the highest percentage increases in the region due to their historical foment.

RESUMEN Una de las funciones más importantes que cumplen los bosques, es su capacidad para almacenar carbono, esta varía de acuerdo con su composición florística, edad y densidad del arbolado. Con la finalidad de aportar información que sea útil para los inventarios de carbono, se plantearon como objetivos la construcción de curvas de crecimiento para estratos de dominancia dentro del área de estudio. Con los datos del Inventario Nacional Forestal y de Suelos 2005-2009 se calculó el IVI por conglomerado y a partir de un dendrograma derivado de un análisis de agrupamiento usando el método de ligamiento de Ward, se formaron estratos de dominancia. Se construyó una curva de crecimiento por estrato, partiendo de los rendimientos máximos por hectárea registrados en los programas de manejo forestal y se atribuyeron los incrementos porcentuales en volumen por especie a diferentes edades, estos obtenidos a partir del ICA mediante análisis troncales. Fueron formados 5 estratos de dominancia; Pinus oocarpa con valores de 21,08 % a 54,38 %, Pinus douglasiana 41,45 % a 89,83 %, Pinus devoniana 27,53 % a 63,88 %, Pinus leiophylla 27,53 % a 89,03 % y Quercus spp. 36,05 % a 93,26 %. Los rendimientos máximos para los rodales dentro del área de estudio fueron 329,98 m3 ha-1, 330,71 m3 ha-1, 171,35 m3 ha-1, 185.22 m3 ha-1 y 127,46 m3 ha-1 respectivamente. Los estratos de Pinus oocarpa y Pinus douglasiana son los de mayor incremento porcentual en la región, debido a su impulso histórico.

RESUMEN La altura dominante de los árboles de un sitio es un indicador indirecto de la productividad de los suelos forestales y se le denomina calidad de estación, la cual se mide a través del índice de sitio. El objetivo fue ajustar modelos de altura dominante en función de la edad con enfoque de modelos de efectos mixtos para generar curvas de índice de sitio en plantaciones forestales de Pinus oocarpa Shiede en Ario de Rosales, Michoacán, México. Con 1 773 pares de datos altura dominante-edad de plantaciones forestales de entre dos a 20 años y relictos de 56 años, se ajustaron cinco modelos de crecimiento para elegir uno base. El modelo de Weibull se ajustó por modelos de efectos mixtos con la parcela como covariable aditiva en el parámetro relacionado al sitio. La curva guía en altura dominante y las curvas polimórficas cubren adecuadamente la variabilidad muestral de la información y representan de forma confiable la productividad del sitio donde crecen estas plantaciones. El sesgo y la validación de los resultados mostró confiabilidad en las estimaciones. El turno técnico en altura dominante está entre cinco y 15 años de acuerdo con su calidad de estación. Con estos resultados es posible realizar la clasificación de las plantaciones forestales de Pinus oocarpa de manera confiable y acorde con el nivel de productividad de cada sitio de establecimiento.

ABSTRACT The dominant height of the trees in a site is an indirect indicator of the productivity of forest soils is called station quality, which is measured through the site index. The aim of this study was to fit Ad models as a function of age with a mixed effects modeling approach to generate site index curves in forest plantations of Pinus oocarpa Shiede in Ario de Rosales, Michoacán, Mexico. With 1 773 pairs of forest plantation dominant height-age data from two to 20 years old and 56 years old relicts, five growth models were fitted to choose a base model. The Weibull model was fitted by mixed effects modeling with the plot as an additive covariate in the site-related parameter. The dominant height guide curve and the polymorphic curves adequately cover the sample variability of the information and reliably represent the productivity of the site where these plantations grow. The bias and validation of the results showed reliability in the estimates. The technical shift in dominant height is between 5 and 15 years according to the quality of the season. With these results, it is possible to classify Pinus oocarpa forest plantations reliably and according to the level of productivity of each establishment site.

Resumen Este trabajo analiza la relación de emisiones agrícolas de metano y óxido nitroso en función del producto interno bruto per cápita y su término cuadrático en 26 países latinoamericanos y del Caribe (1990-2019); considera también el stock de inversión extranjera para probar hipótesis del paraíso contaminante, la superficie agrícola (SA) y el metano para la función de óxido nitroso. El estudio comprendió pruebas de raíz unitaria y cointegración. La estimación de coeficientes se realizó por mínimos cuadrados completamente modificados (FMOLS), efectos comunes correlacionados (CCE) y grupo medio aumentado (AMG). Los resultados por FMOLS muestran evidencia de una probable curva ambiental de Kuznets en el conjunto de países para metano, además sustentan un paraíso contaminante. Los resultados por CCE y AMG indican lo contrario. Para óxido nitroso los resultados de FMOLS apoyan una curva en U. Lo anterior sostiene una política ambiental conjunta de ambos gases, donde la SA es un elemento clave. Los resultados anteriores se tienen que corroborar con otros métodos y extender los estudios a otros países en el tiempo.

Abstract This paper aims to analyze the relationship between agricultural methane and nitrous oxide emissions as a function of per capita gross domestic product (GDP) and its quadratic term in 26 Latin American and Caribbean countries (1990-2019). It also considers the stock of foreign investment to test the polluting paradise hypothesis, agricultural land (AL), and, additionally, methane for nitrous function. The study comprised unit root and cointegration tests. Coefficients were estimated using fully modified least squares (FMOLS), correlated common effects (CCE), and augmented mean group (AMG). Results by FMOLS show statistical evidence of an environmental Kuznets curve in the set of countries for methane and support the polluting paradise hypothesis. However, results from CCE and AMG show the opposite. For nitrous oxide, FMOLS support a U-shaped curve. The above supports a joint environmental policy for both gases, where al is a key element. The previous results must be corroborated with other methods and the studies extended to other countries over time.

Resumen Los herbicidas sintéticos han sido la herramienta más utilizada para el control de las plantas arvenses desde su descubrimiento y desarrollo a mediados del siglo XX. Sin embargo, su uso continuado ha llevado a la aparición de biotipos de arvenses resistentes, disminuyendo su eficacia. Además, han causado problemas medioambientales, afectan a especies no diana, y pueden producir fitotoxicidad en los cultivos. Por ello, es necesario buscar nuevas materias herbicidas que sean efectivas en el control de las arvenses pero respetuosas con el medio ambiente. Los aceites esenciales de algunas plantas poseen compuestos con propiedades fitotóxicas, que pueden inhibir la germinación y el crecimiento de las arvenses. El carvacrol es un fenol monoterpénico que se encuentra principalmente en los aceites esenciales de las plantas de la familia Lamiaceae, con demostrada actividad herbicida. El objetivo del presente trabajo fue obtener una curva dosis-respuesta del carvacrol sobre las especies Lolium rigidum (modelo de arvense monocotiledónea) y Sonchus oleraceus (modelo de arvense dicotiledónea). Los ensayos se realizaron en invernadero, aplicando distintas dosis de carvacrol sobre las arvenses estudiadas a fin de obtener la curva dosis-respuesta de cada una de ellas. Para evaluar la actividad herbicida del carvacrol se determinó su eficacia. S. oleraceus fue más sensible al carvacrol que L. rigidum. La obtención de estas curvas dosis-respuesta permitirá en el futuro utilizarlas como referencia para realizar ensayos en condiciones de campo y para el estudio de interacciones sinérgicas del carvacrol con otros compuestos.

Abstract Synthetic herbicides have been the most widely used tool for weed control since their discovery and development in the mid-20th century. However, their continued use has led to the emergence of resistant weed biotypes, reducing their efficacy. They also provoked environmental problems, affecting non-target species, and causing crop stress. It is therefore necessary to look for new herbicides that could be effective in controlling weeds while also being environmentally friendly. The essential oils of some plants have compounds with phytotoxic properties, which can inhibit the germination and growth of weeds. Carvacrol is a monoterpene phenol found mainly in the essential oils of plants of the Lamiaceae family, with proven herbicidal activity. The aim of the present work was to obtain a dose-response curve of carvacrol on Lolium rigidum (monocotyledonous weed model) and Sonchus oleraceus (dicotyledonous weed model). The tests were carried out under greenhouse conditions, applying different doses of carvacrol on the weeds studied to obtain the dose-response curves. To assess the herbicidal activity of carvacrol, its efficacy on weeds were determined. S. oleraceus was more sensitive to carvacrol than L. rigidum. The dose-response curves obtained will be used in the future as a reference for field trials and for the study of synergistic interactions of carvacrol with other compounds.

RESUMEN No existe consenso sobre la curva de crecimiento a utilizar para evaluar el crecimiento fetal. Validar unas curvas personalizadas y estudiar su rendimiento en la detección de neonatos con restricción de crecimiento intrauterino (RCIU), así como su precisión diagnóstica. Inicialmente se diseñó unas curvas personalizadas con 2,792 fetos únicos de embarazos de riesgo bajo; se calculó el peso óptimo a las 40 semanas = 1,496.202 + (64.379 x sexo fetal) + (831.362 x talla materna) + (9.567 x peso pregestacional), ecuación que se combinó con una función de proporcionalidad estándar para ajustar los pesos según su edad gestacional. Posteriormente se evaluó su rendimiento aplicándola en una cohorte retrospectiva de neonatos de 24 a 40 semanas nacidos entre 2018 y 2022 en un hospital de tercer nivel de Lima, Perú. Se excluyeron gemelos y anomalías congénitas. Se estudió 6,598 neonatos. Las curvas personalizadas mostraron buena concordancia con INTERGROWTH-21 (IG21) (kappa = 0,68; IC95% = 0,62 a 0,74). Se detectó un 2,8% de RCIU (184/6,598), similar al 3,1% de IG21 (205/6,598). Las curvas mostraron alta especificidad y valor positivo negativo (VPN) (97% y 98%; IC95% = 97 a 98% y 98 a 99%, respectivamente). El riesgo para muerte perinatal (RR =7,2; IC95% = 4,6 a 11) y su exactitud (96; IC95% = 95 a 96%) fueron superiores a los de la Fundación de Medicina Fetal (FMF) (RR = 3,6; IC95% = 2,5 a 5,2 y exactitud = 89%; IC95% = 88 a 89%, respectivamente). Las curvas peruanas personalizadas resultaron fiables para evaluar la RCIU. Su capacidad de detección y su precisión diagnóstica fueron similares a otras curvas internacionales, aunque algo superiores a las de la FMF.

ABSTRACT There is no consensus on the growth curve to be used to assess fetal growth. To validate customized curves and study their performance in the detection of neonates with intrauterine growth restriction (IUGR), as well as their diagnostic accuracy. Initially, customized curves were designed with 2,792 singleton fetuses from low-risk pregnancies; the optimal weight at 40 weeks = 1,496.202 + (64.379 x fetal sex) + (831.362 x maternal length) + (9.567 x pregestational weight) was calculated and combined with a standard proportionality function to adjust the weights according to gestational age. Subsequently, its performance was evaluated by applying it in a retrospective cohort of neonates aged 24-40 weeks born between 2018-2022 in a tertiary hospital in Lima-Peru. Twins and congenital anomalies were excluded. A total of 6,598 neonates were studied. Customized curves showed good agreement with INTERGROWTH-21 (IG21) (kappa = 0.68; 95%CI = 0.62-0.74). They detected 2.8% of IUGR (184/6,598), similar to the 3.1% for IG21 (205/6,598). They showed high specificity and negative positive value (NPV) (97% and 98%; 95%CI = 97-98% and 98-99%, respectively). The risk for perinatal death (RR = 7.2; 95%CI = 4.6-11) and accuracy (96; 95%CI = 95-96%) were higher than those of the Fetal Medicine Foundation (FMF) (RR=3.6; 95%CI = 2.5-5.2 and accuracy= 89%; 95%CI = 88-89%, respectively). The customized Peruvian curves were reliable in assessing IUGR. Their detection capacity and diagnostic accuracy were similar to other international curves, although somewhat higher than those of the FMF.

ABSTRACT Introduction: Fetal ultrasound has achieved a central role in the modern diagnosis of fetal growth deviations. Objective: To determine the local discrimination power and the performance of fetal biometric variables at 33 weeks with respect to the trophic condition of the newborn. Methods: An observational, analytical and retrospective study was carried out in three health areas of Santa Clara Municipality, in the period between January 2013 and December 2019. From a population of 6035 births, 2454 were selected by simple random sampling. Data were obtained from records of genetics consultations. Areas were constructed under the receiver operating characteristic curve and performance indicators for diagnostic tests were calculated. Results: The areas under the curve of the biometric variables discriminate between those born small or large based on gestational age. Local cut-off points are estimated. Biometrics performance indicators maintain regular behavior; those calculated by transforming the values from the reference tables are more specific, while those calculated by transforming the variables by the estimated cut-off points increase sensitivity. Conclusions: All the biometric variables have some discriminatory capacity for deviations from the trophic condition at birth, higher for those born small based on gestational age. The performance indicators of the fetal biometric variables showed superiority according to the estimated cut-off points compared to those of the reference tables.

RESUMEN Introducción: El ultrasonido fetal ha logrado un papel central en el diagnóstico moderno de las desviaciones de crecimiento fetal. Objetivo: Determinar el poder discriminatorio local y desempeño de variables biométricas fetales a las 33 semanas sobre la condición trófica del recién nacido. Métodos: Se realizó estudio observacional, analítico y retrospectivo en tres áreas de salud del municipio Santa Clara, en el período comprendido entre enero de 2013 a diciembre del 2019. De una población de 6035 nacidos se seleccionaron 2454 por muestreo simple aleatorio. Se obtuvieron datos de registros de las consultas de genética. Se construyeron áreas bajo la curva Receiver Operating Characteristic y se calcularon indicadores de desempeño para pruebas diagnósticas. Resultados: Las áreas bajo la curva de las variables biométricas discriminan a los nacidos pequeños y grandes para la edad gestacional. Se estiman puntos de corte locales. Los indicadores de desempeño de la biometría mantienen un comportamiento regular; los calculados al transformar los valores a partir de las tablas de referencia son más específicos; y los calculados al transformar las variables por los puntos de corte estimados elevan la sensibilidad. Conclusiones: Todas las variables biométricas tienen capacidad discriminatoria para las desviaciones de la condición trófica al nacer, superior para los nacidos pequeños para la edad gestacional. Los indicadores de desempeño de las variables biométricas fetales demostraron superioridad según los puntos de corte estimados respecto a los de las tablas de referencia.

ABSTRAC Introduction: dentition and growth curve are indicative of the maturation of the infant. This knowledge is of anthropological, demographic, forensic and paleontological interest; it allows us to diagnose, develop treatment plans and predict prognoses. It is useful to keep in mind the average eruption pattern to determine if there are noticeable advances or delays, and greater variability is found in chronology than in sequence. In Cuba, national standards for the order and chronology of dental eruption have not been established. Objective: to describe the patterns of the order and chronology of eruption of deciduous dentition. Methods: a descriptive epidemiological study of deciduous dentition was carried out in children from Villa Clara province between 2014 and 2018. A sample of 520 children between 3 months and 4 years old who were born in the province without alterations in general and craniofacial growth was obtained through a simple random multistage sampling; the eruption was observed and weighted averages for ages were calculated. Results: differences were found for ages; symmetry prevailed in more than half according to Mayoral, Moyers and Águila as well as lower teeth erupted before their upper counterparts, except for canines; central incisors, lateral incisors, 1st molars, canines and 2nd molars was the most frequent order. Conclusions: tooth eruption occurred in stages; they included certain dental groups separated by periods of rest and polymorphic sequences were found in a small number of subjects. A table with the eruption ages adjusted to Villa Clara population was prepared.

RESUMEN Introducción: La dentición y la curva de crecimiento son orientativas de la maduración del infante. Este conocimiento es de interés antropológico, demográfico, forense y paleontológico; permite diagnosticar, elaborar planes de tratamiento y prever pronósticos. Es útil tener presente el patrón de erupción promedio para determinar si hay adelantos o retrasos notorios, y se encuentra mayor variabilidad en la cronología que en la secuencia. En Cuba no han sido establecidos los estándares nacionales de orden y cronología de brote dentario. Objetivo: Describir los patrones del orden y cronología de brote de la dentición temporal. Métodos: Se realizó un estudio epidemiológico descriptivo de la dentición temporal en niños de la provincia de Villa Clara, entre los años 2014 a 2018. Mediante un muestreo polietápico aleatorio simple, se obtuvo una muestra de 520 niños entre 3 meses y 4 años, nacidos en la provincia, sin alteraciones del crecimiento general y craneofacial; se observó el brote y se calcularon los promedios ponderados para las edades. Resultados: Se encontraron diferencias para las edades; según Mayoral, Moyers y Águila, en más de la mitad, predominó la simetría y los dientes inferiores brotaron antes que sus homólogos superiores, excepto los caninos; el orden más frecuente fue: incisivos centrales, incisivos laterales, 1ros molares, caninos y 2dos molares. Conclusiones: El brote ocurrió por etapas; incluyeron determinados grupos dentarios separados por periodos de reposo y las secuencias polimórficas fueron encontradas en poca cantidad de sujetos. Se elaboró una tabla de edades de brote ajustada a la población villaclareña.

Resumo O objetivo do estudo foi avaliar o estado nutricional antropométrico e o crescimento físico de crianças Terena residentes na área urbana de Campo Grande, Mato Grosso do Sul, no primeiro ano de vida. Participaram da pesquisa crianças nascidas de junho de 2017 a julho de 2018 (n = 42). As curvas de crescimento foram construídas por modelos aditivos generalizados mistos. Aos 12 meses, 4,8% das crianças apresentaram baixa E/I. Segundo o índice de massa corporal para a idade, o sobrepeso atingiu 15,0% das meninas aos 12 meses e a obesidade 4,8% dos meninos e meninas nessa idade. A curva de comprimento das crianças Terena não alcançou a mediana da população de referência. A curva de peso e índice da massa corporal para a idade, especialmente entre as meninas, seguiram, em geral, uma trajetória superior à mediana de referência. As curvas de crescimento Terena registram um crescimento linear médio inferior ao esperado e um ganho de peso superior ao da mediana de referência. Esse perfil é compatível com a persistência de condições desfavoráveis ao crescimento e à nutrição infantis e com um acelerado processo de transição alimentar e nutricional, em que a dimensão racial das iniquidades em saúde não pode ser desconsiderada.

Resumen Este estudio tuvo como objetivo evaluar el estado nutricional antropométrico y el crecimiento físico de los niños Terena residentes en el área urbana de Campo Grande, Mato Grosso do Sul, Brasil, en el primer año de vida. Participaron del estudio niños nacidos entre junio de 2017 y julio de 2018 (n = 42). Los datos se recopilaron durante visitas domiciliarias en el 1º, 6º y 12º mes de vida del niño. Las curvas de crecimiento se construyeron utilizando modelos mixtos aditivos generalizados. A los 12 meses, el 4,8% de los niños tenían T/E baja. Según el índice de masa corporal por edad, el sobrepeso afectaba al 15,0% de las niñas a los 12 meses y la obesidad afectaba al 4,8% de los niños y niñas de esta edad. La curva de talla de los niños Terena no alcanzó la mediana de la población de referencia. La curva de peso y del índice de masa corporal por edad, especialmente entre las niñas, siguió en general una trayectoria superior a la mediana de referencia. Las curvas de crecimiento de Terena registran un crecimiento lineal medio inferior al esperado y una ganancia de peso superior a la mediana de referencia. Este perfil es compatible con la persistencia de condiciones desfavorables para el crecimiento y la nutrición infantil y con un proceso acelerado de transición alimentaria y nutricional, donde no se puede desconocer la dimensión racial de las inequidades en salud.

Abstract The present study aimed to assess the anthropometric nutrition status and physical growth of Terena Indigenous children living in an urban zone of the city of Campo Grande, Mato Grosso do Sul, Brazil, in their first year of life. Children who were born between June 2017 and July 2018 (n = 42) participated in the study. In total, 4.8% of the children presented a low height for age (H/A) in the 12th month of life. According to body mass index (BMI) for age, overweight affected 15.0% of the individuals belonging to the female sex at the age of 12 months, and obesity was observed in 4.8% of the children belonging to both sexes at this same age. Terena Indigenous children’s length curve failed to reach the median value recorded for the reference population. Weight and body mass index curves for age, mainly among individuals belonging to the female sex, were often above the reference median value. The Terena growth curves recorded an average linear growth of lower than expected and a weight gain of higher than the reference median. This profile is compatible with the persistence of unfavorable conditions for children’s growth and nutrition, as well as with an accelerated process of food and nutritional transition, where the racial dimension of health inequities cannot be disregarded.

Abstract Piaractus mesopotamicus, is a fish usually farmed in semi-intensive systems with access to natural food and supplementary feed. This study evaluates effects of feed allowance on the productive performance, carbon turnover and proportions of nutrient (carbon) contribution of feed and natural food for the growth of pacu. Juvenile fish were stocked in fiberglass tanks and fed to 100, 75, 50, 25, 0% apparent satiety (ApS), with a practical, extruded (C4 photosynthetic pathway) feed in a randomized design trial (n=3); plankton production for simulated semi-intensive farming system condition was induced by chemical fertilization. A control treatment was set up in tanks devoid of natural food. Data on muscle stable carbon isotope ratios were used to study carbon turnover using a relative growth-based model. Low variation of the δ13C impaired fitting a turnover model curve for the 0 and 25 % ApS treatments. Fish of the 100% and 75% ApS treatments reached circa 95% and 82.85% of the carbon turnover, respectively. Extruded feed was the main nutrient source for the growth of pacu in the semi-intensive, simulated farming condition. The current study contributes to the knowledge of the relationship between feeding rates and carbon turnover rates in the pacu muscle. mesopotamicus semiintensive semi intensive performance (carbon 100 75 50 ApS, , (ApS) practical C4 C (C pathway n=3 n3 n 3 (n=3) fertilization growthbased based δC δ 2 95 8285 82 85 82.85 respectively semiintensive, intensive, 10 7 5 (ApS n= (n=3 9 828 8 82.8 1 (n= 82. (n

RESUMO Nanopartículas de óxido metálico (NPs) possuem atividade antibacteriana e podem ser utilizadas para o controle de bactérias fitopatogênicas. O objetivo deste trabalho foi avaliar a atividade antibacteriana de NPs puras e dopadas contra Dickeya zeae, Pantoea ananatis e Xanthomonas vasicola pv. vasculorum in vitro, e avaliar a eficácia da aplicação preventiva e curativa de NPs de óxido de zinco (ZnO), óxido de níquel (NiO) e óxido de zinco dopado com prata (ZnO:Ag) para o controle da mancha branca (WS) e estria bacteriana (BLS) in vivo. A inibição do crescimento bacteriano foi primeiramente avaliada medindo o diâmetro da zona de inibição formada em placas de Petri e a severidade das doenças WS e BLS foi avaliada em casa de vegetação, calculando a área abaixo da curva de progresso da doença. A atividade antibacteriana in vitro não foi influenciada pelo aumento na concentração dos elementos dopantes para a maioria das NPs. NPs de ZnO dopadas com Ag, K e Mo; ZnOCl dopada com Ag e NPs puras (Ag2O, CuO e NiO) apresentaram atividade antibacteriana contra D. zeae, P. ananatis e X. vasicola pv. vasculorum com halos de inibição relativamente semelhantes em diferentes concentrações. O cobre comercial apresentou atividade antibacteriana apenas para D. zeae. NPs de NiO em aplicações preventivas e curativas reduziram a severidade de WS e BLS, enquanto a aplicação de cobre comercial aumentou a severidade de WS e reduziu a severidade de BLS. O uso de NPs tem aplicações promissoras na cultura do milho e estudos futuros avaliando sua formulação, forma de aplicação e época são necessários para novas estratégias de controle de bactérias fitopatogênicas. (NPs fitopatogênicas zeae pv ZnO, , (ZnO) (NiO ZnOAg (ZnO:Ag (WS (BLS vivo vegetação doença Mo Ag2O, Ag2O AgO (Ag2O D P X concentrações formulação (ZnO

ABSTRACT Metal oxide nanoparticles (NPs) possess antibacterial activities and can be used for the control of phytopathogenic bacteria. The objective of this work was to evaluate the antibacterial activity of pure and doped NPs against Dickeya zeae, Pantoea ananatis, and Xanthomonas vasicola pv. vasculorum in vitro, and to assess the efficacy of preventive and curative application of zinc oxide (ZnO), nickel oxide (NiO), and silver-doped zinc oxide (ZnO:Ag) NPs for the control of white spot (WS) and bacterial leaf streak (BLS) in vivo. Bacterial growth inhibition was first evaluated by measuring the diameter of the inhibition zone formed in Petri dishes. Subsequently, the severity of WS and BLS diseases was evaluated in a greenhouse calculating the area under the disease progress curve. The in vitro antibacterial activity was not influenced by the increase in the concentration of doping elements for most NPs. ZnO NPs doped with Ag, K, and Mo; ZnOCl doped with Ag, and pure NPs (Ag2O, CuO, and NiO) showed antibacterial activity against D. zeae, P. ananatis, and X. vasicola pv. vasculorum with relatively similar inhibition zones at different concentrations. Commercial copper showed antibacterial activity only against D. zeae. NiO NPs in preventive and curative applications reduced WS and BLS severities, whereas commercial copper application increased WS severity and reduced BLS severity. The use of NPs has promising applications and further evaluation of their formulation, application form, and timing is necessary for new strategies to control the activity of phytopathogenic bacteria. (NPs bacteria zeae ananatis pv ZnO, , (ZnO) NiO, (NiO) silverdoped silver ZnOAg Ag (ZnO:Ag (WS (BLS vivo dishes Subsequently curve K Mo Ag2O, Ag2O AgO O (Ag2O CuO D P X concentrations severities formulation form (ZnO (NiO