The objective of this work was to evaluate the total concentration and bioaccessible fractions of Ba, Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Pb, V and Zn in berry fruits and analyze the influence of different cultivars and growing conditions on the obtained results. The variations in bioaccessible concentrations were from 7.7-30, 17-29, 22-50, 1.8-96, 22-33 and 20-51% of the total concentration, for Ba, Cu, Fe, Mn, Pb and Zn, respectively. Copper showed greater bioaccessibility in blackberry and blueberry. For Fe, bioaccessibility was observed in all strawberries and blueberries and in some blackberries. Zinc presented higher bioaccessible fractions in strawberries. For V, only the sample Blackberry 124 showed bioaccessible concentration, (11% of the total concentration). Regarding the different forms of cultivation, the strawberries that received radiation from the red and white lamps presented a higher production of fruit compared to the other systems, however, in relation to the absorption of nutrients, a small variation was observed between the fruits. Ba Ca Cd Cr Cu Fe K Mg Mn results 7.730, 7730 7.7 30, 7 30 7.7-30 1729, 1729 17 29, 29 17-29 2250, 2250 22 50, 50 22-50 1.896, 1896 1.8 96, 1 8 96 1.8-96 2233 33 22-3 2051% 2051 20 51% 51 20-51 respectively blueberry blackberries 12 11% 11 (11 concentration. . concentration) cultivation systems however nutrients 730 7.730 773 77 7. 3 7.7-3 172 2 17-2 225 5 22-5 896 1.896 189 18 1. 9 1.8-9 223 22- 205 20-5 (1 73 7.73 7.7- 17- 89 1.89 1.8- 20- (

RESUMO O pimentão, cultura de importância nutricional, destaca-se por seu valor de vitamina C. Diante da necessidade de práticas agrícolas sustentáveis, o estudo explorou o uso de biocarvões derivados de resíduos regionais como alternativa na cultura de pimentão. Nesse contexto, objetivou-se avaliar o efeito de biocarvões provenientes de cama de frango, fibra de coco e palha de arroz, nas características biométricas e físico-químicas de frutos de pimentão verde, na produção de frutos por planta e na produtividade. A pesquisa foi conduzida em campo no delineamento em blocos casualizados, em esquema fatorial 5 × 3 + 1, referentes a cinco doses de biocarvão (0, 3, 6, 9 e 12 t ha-1) e três tipos de biocarvão (fibra de coco, cama de aviário e palha de arroz) com quatro repetições, mais um tratamento adicional (adubação mineral). Foram avaliadas variáveis biométricas (comprimento e diâmetro dos frutos, espessura da casca, número de frutos, massa do fruto) e físico-químicas dos frutos (sólidos solúveis, acidez titulável, pH, vitamina C e teor de cinzas), produção e produtividade dos frutos. A produção de frutos de pimentão verde não foi influenciada pelos biocarvões, porém as doses de biocarvões influenciaram todas as variáveis analisadas com exceção da espessura da casca. Os teores de vitamina C e de sólidos solúveis foram maiores nos tratamentos com biocarvões e menores com adubação mineral. A maior influência na acidificação dos frutos foi com o biocarvão produzido com palha de arroz. nutricional destacase destaca se sustentáveis contexto objetivouse objetivou frango arroz físicoquímicas físico químicas casualizados 1 0, 0 (0 6 ha1 ha ha-1 repetições mineral . mineral) comprimento casca fruto titulável pH cinzas, cinzas , cinzas) ( ha-

ABSTRACT Bell pepper, a crop of nutritional importance, stands out for its vitamin C content. Given the need for sustainable agricultural practices, the present study explored the use of biochars derived from regional waste as an alternative in bell pepper cultivation. In this context, the present study aimed to evaluate the effect of biochars from poultry litter, coconut fiber, and rice straw on the biometric and physicochemical characteristics of bell pepper fruits, on fruit production per plant and on fruit yield. The experiment was performed in the field in a randomized block design, in a 5 × 3 + 1 factorial scheme, referring to five doses of biochar (0, 3, 6, 9, and 12 t ha-1) and three types of biochar (coconut fiber, poultry litter, and rice straw) with four repetitions, plus an additional treatment (mineral fertilization). Biometric variables (fruit length and diameter, skin thickness, number of fruits, fruit mass) and physicochemical variables of the fruits (soluble solids, titratable acidity, pH, vitamin C, and ash content), production and yield of the fruits were evaluated. The production of bell pepper fruits was not influenced by biochars, but the doses of biochars influenced all variables analyzed with the exception of skin thickness. Vitamin C and soluble solids contents were higher in treatments with biochar and lower with mineral fertilizer. Biochar produced from rice straw had the greatest influence on fruit acidification. importance content practices cultivation context litter fiber design scheme 0, 0 (0 6 9 ha1 ha ha-1 repetitions fertilization. fertilization . fertilization) diameter thickness mass acidity pH content, , content) evaluated fertilizer acidification ( ha-

The history of the discovery, use, and development of dyes is closely linked to the history of humanity itself. Among the first records of human civilization are cave paintings made from, e.g., fruits, plants, minerals, and insects. Over the centuries, using different techniques, colors have been given to all types of materials and products, from clothing to food. The chemicals responsible for colors are dyes - a vast collection of molecules of the most varied compositions, but which have in common the absorption of light within the visible window of the electromagnetic spectrum. This article aims not only to review the properties of some classes of natural dyes, their origins, and their applications but also to describe the various computational chemistry methods involved in designing new bio-inspired molecules. discovery use itself eg e g e.g. fruits plants minerals insects centuries techniques products food compositions spectrum origins bioinspired bio inspired e.g

RESUMO Regiões com climas semiáridos apresentam condições ambientais que resultam em estresses abióticos às plantas. A maior área de produção de melão (Cucumis melo L.) do Brasil, o Estado do Rio Grande do Norte, possui essas características climáticas. O uso de bioestimulantes nesses sistemas de produção pode mitigar os efeitos dos estresses abióticos e aumentar a produtividade e a qualidade dos frutos. O objetivo neste estudo foi avaliar as características fisiológicas e de produção do meloeiro, sob aplicação de bioestimulantes. Os bioestimulantes Acadian®, Folicist® e Nov@® foram aplicados e comparados ao tratamento controle (sem bioestimulantes), em melões ‘Goldex’ e ‘McLaren’. Nov@® proporcionou maior assimilação de CO2, transpiração e condutância estomática, assim como ascorbato peroxidase em ‘Goldex’ e ‘McLaren’. Maior catalase foi obtida pela aplicação de Nov@®, enquanto que o uso de Folicist® resultou em maior teor de malondialdeído. A cultivar McLaren apresentou o melhor desempenho fisiológico e bioquímico e a ‘Goldex’ o melhor rendimento. Folicist® promove adaptações fisiológicas aos estresses sem prejudicar a atividade fotossintética. Os bioestimulantes aumentaram a produtividade e o número de frutos por planta e promoveram adaptações fisiológicas às condições semiáridas. plantas Cucumis L. L Brasil Norte climáticas meloeiro Acadian Acadian® Folicist Nov Nov@ bioestimulantes, , bioestimulantes) ‘Goldex Goldex ‘McLaren. ‘McLaren . ‘McLaren’ CO2 CO estomática malondialdeído rendimento fotossintética semiáridas

ABSTRACT Regions with semi-arid climates have environmental conditions that result in abiotic stress to plants. The largest melon (Cucumis melo L.) production area in Brazil, the state of Rio Grande do Norte, has these climatic characteristics. Use of biostimulants in these production systems can mitigate effects of abiotic stress and increase yield and fruit quality. The objective in this study was to evaluate the physiological and production characteristics of melon, under the application of biostimulants. The biostimulants Acadian®, Folicist® and Nov@® were applied and compared to the control treatment (no biostimulants), in ‘Goldex’ and ‘McLaren’ melons. Nov@® led to higher CO2 assimilation, transpiration and stomatal conductance, as well as ascorbate peroxidase, in ‘Goldex’ and ‘McLaren’. Greater catalase was obtained with the application of Nov@®, while the use of Folicist® resulted in higher malondialdehyde content. The cultivar ‘McLaren’ exhibited the best physiological and biochemical performance, and ‘Goldex’ the best yield. Folicist® promotes physiological adaptations to stress without impairing photosynthetic activity. The biostimulants increased yield and number of fruits per plant and promoted physiological adaptations to semi-arid conditions. semiarid semi arid plants Cucumis L. L Brazil Norte quality Acadian Acadian® Folicist Nov Nov@ no biostimulants, , biostimulants) ‘Goldex Goldex ‘McLaren McLaren melons CO assimilation conductance peroxidase ‘McLaren. . content performance activity

RESUMO A densidade de plantas e o espaçamento correto são fatores fundamentais para alcançar produtividade rentável e qualidade dos frutos em culturas agrícolas. Este estudo teve como objetivo avaliar a produtividade do tomateiro de crescimento determinado e as características físico-químicas dos frutos, em diferentes arranjos de plantio e densidades de plantas. Foram realizados dois experimentos, sendo que no primeiro experimento e no segundo experimento foram utilizados os híbridos CVR-2909 (CVR Plant Breeding) e HMX 7885 (Agristar), respectivamente. Os experimentos foram conduzidos em delineamento de blocos casualizados, com quatro repetições. Foram avaliados dez tratamentos em esquema fatorial duplo (2 × 5). No primeiro fator, foram avaliados dois arranjos de plantio: linhas simples e linhas duplas. No segundo fator, foram avaliadas cinco densidades de plantas: 15, 20, 25, 30 e 35 mil plantas ha-1. Para o híbrido CVR-2909, o cultivo em linhas simples proporcionou maior produtividade, já as maiores densidades de plantas resultaram em menor produção e número de frutos por planta. Para o híbrido HMX-7885, maiores densidades de plantas proporcionaram maior produtividade, porém com menor produção por planta, massa média e número de frutos por planta. Para esse híbrido o arranjo de plantio proporcionou maior taxa de frutos verdes. agrícolas físicoquímicas físico químicas CVR2909 CVR 2909 CVR-290 Breeding 788 Agristar, Agristar , (Agristar) respectivamente casualizados repetições 2 ( 5. 5 . 5) fator duplas 15 20 25 3 ha1. ha1 ha 1. 1 ha-1 CVR2909, 2909, planta HMX7885, HMX7885 7885, HMX-7885 verdes CVR290 290 CVR-29 78 (Agristar ha- HMX788 HMX-788 CVR29 29 CVR-2 7 HMX78 HMX-78 CVR2 CVR- HMX7 HMX-7 HMX-

ABSTRACT Plant density and correct row spacing are fundamental factors for achieving profitable yield and fruit quality in crops. This study aimed to evaluate the yield of tomato plants of determinate growth and the physicochemical characteristics of the fruits in different planting arrangements and plant densities. Two experiments were conducted using the hybrids CVR-2909 (CVR Plant Breeding) and HMX 7885 (Agristar) in the first and second experiments, respectively. The experiments were conducted in a randomized block design with four replicates. Ten treatments were evaluated in a double factorial scheme (2 × 5). Two planting arrangements were evaluated in the first factor: single and double rows. The second factor comprised five plant densities: 15, 20, 25, 30, and 35 thousand plants ha-1. For the CVR-2909 hybrid, single-row cultivation resulted in the highest yield, while higher planting densities gave the lowest yield and number of fruits per plant. For the HMX-7885 hybrid, it was found that higher densities gave higher yields but caused a decreasing effect on fruit production per plant, average fruit mass, and number of fruits per plant. For this hybrid, the planting arrangement provided a higher rate of green fruits. crops CVR2909 CVR 2909 CVR-290 Breeding 788 Agristar (Agristar respectively replicates 2 ( 5. 5 . 5) rows 15 20 25 30 3 ha1. ha1 ha 1. 1 ha-1 hybrid singlerow HMX7885 HMX-788 mass CVR290 290 CVR-29 78 ha- HMX788 HMX-78 CVR29 29 CVR-2 7 HMX78 HMX-7 CVR2 CVR- HMX7 HMX-

RESUMO: O tomate (Solanum lycopersicum L.) é uma fruta rica em nutrientes e com alto teor de ácido glutâmico, tornando-o uma fonte adequada para a produção de ácido γ-aminobutírico (GABA) através da ação de bactérias ácido lácticas (BAL). As BAL desempenham um papel fundamental na fermentação láctica, melhorando o sabor e o valor nutricional dos produtos alimentares, contribuindo frequentemente para os seus benefícios para a saúde. Este estudo teve como objetivo isolar BAL produtoras de GABA de tomates e avaliar seu potencial para a produção de suco de tomate fermentado enriquecido com GABA. Os resultados indicam o isolamento de 15 cepas de BAL a partir de amostras de cinco tomates diferentes. Todas essas cepas demonstraram capacidade de produzir GABA, com níveis variando de 1,732 a 3,113 mg/mL, conforme determinado por cromatografia em camada delgada (CCD). Através de sequenciamento, a cepa promissora TO42, identificada como Weissella cibaria, foi selecionada para fermentação de suco de tomate. Além disso, o suco de tomate enriquecido com GABA foi fermentado com sucesso a 15 °Brix por 72 h, resultando no maior teor registrado de GABA e ácido láctico de 9.185 ± 0.398 mg/mL e 13.05 ± 1.56 g/L, respectivamente. RESUMO Solanum L. L glutâmico tornandoo tornando γaminobutírico γ aminobutírico (GABA BAL. . (BAL) láctica alimentares saúde 1 diferentes 1732 732 1,73 3113 3 113 3,11 mgmL mg mL CCD. CCD (CCD) sequenciamento TO42 TO cibaria disso Brix 7 h 9185 9 185 9.18 0398 0 398 0.39 1305 13 05 13.0 156 56 1.5 gL g g/L respectivamente (BAL 173 73 1,7 311 11 3,1 (CCD TO4 918 18 9.1 039 39 0.3 130 13. 5 1. 17 1, 31 3, 91 9. 03 0.

ABSTRACT: Tomatoes (Solanum lycopersicum L.) are nutrient-rich fruits with a high glutamic acid content, making them a suitable source for producing γ-aminobutyric acid (GABA) through the action of lactic acid bacteria (LAB). LAB plays a pivotal role in lactic fermentation, enhancing the taste and nutritional value of food products, often contributing to their health benefits. This study isolated GABA-producing LAB from tomatoes and assessed their potential for producing GABA-enriched fermented tomato juice. The results indicated the isolation of 15 LAB strains from the samples of five different tomatoes. All of these strains demonstrated the capability to produce GABA, with levels ranging from 1.732 to 3.113 mg/mL, as determined using thin-layer chromatography (TLC). Through sequencing, the promising strain TO42, identified as Weissella cibaria, was selected for tomato juice fermentation. Furthermore, GABA-enriched tomato juice was successfully fermented at 15 °Brix for 72 h, resulting in the highest recorded GABA and lactic acid content of 9.185 ± 0.398 mg/mL and 13.05 ± 1.56 g/L, respectively. ABSTRACT Solanum L. L nutrientrich nutrient rich γaminobutyric γ aminobutyric (GABA LAB. . (LAB) fermentation products benefits GABAproducing GABAenriched enriched 1 1732 732 1.73 3113 3 113 3.11 mgmL mg mL thinlayer thin layer TLC. TLC (TLC) sequencing TO42 TO cibaria Furthermore Brix 7 h 9185 9 185 9.18 0398 0 398 0.39 1305 13 05 13.0 156 56 1.5 gL g g/L respectively (LAB 173 73 1.7 311 11 3.1 (TLC TO4 918 18 9.1 039 39 0.3 130 13. 5 1. 17 31 3. 91 9. 03 0.

Brazilian Savanna biodiversity is abundant in native fruits with nutritional and sensory properties with potential technological applications. However, the information available regarding the proprieties of Brazilian native fruits is still limited. This study aimed to analyze the metabolic profile of Brosimum gaudichaudii fruits (BGF) using metabolomics approaches, besides its physicochemical and nutritional proprieties. BGF exhibited protein content at least two times higher than other native fruits. Fifty-five metabolites were identified by the metabolomics approaches, mainly fatty acids (n = 22), carbohydrates (n = 13), organic acids (n = 6), and polyphenols (n = 5). The most abundant constituents were D-fructose (42.97%) for sugars, L-asparagine (6.47%) for amino acids, succinic acid (44.11%) for organic acids, and linolenic acid (3.04%) for fatty acids. Highlighted phytochemicals included chlorogenic acid, α-amyrin, and γ-tocopherol. Additionally, this study revealed that BGF is a good source of vitamin C and dietary fiber and an excellent source of vitamin A. Future studies providing nutritional information and suggesting consumption methods and potential industrial applications are essential to familiarize consumers with these foods. Additionally, clarifying the appropriate planting and harvesting seasons can revive traditional practices, especially for native fruits like BGF. However limited (BGF approaches Fiftyfive Fifty five n 22, 22 , 22) 13, 13 13) 6, 6 6) 5. 5 . 5) Dfructose D fructose 42.97% 4297 42 97 (42.97% sugars Lasparagine L asparagine 6.47% 647 47 (6.47% 44.11% 4411 44 11 (44.11% 3.04% 304 3 04 (3.04% αamyrin, αamyrin α amyrin, amyrin α-amyrin γtocopherol. γtocopherol γ tocopherol. tocopherol γ-tocopherol Additionally A foods practices 2 1 42.97 429 4 9 (42.97 6.47 64 (6.47 44.11 441 (44.11 3.04 30 0 (3.04 42.9 (42.9 6.4 (6.4 44.1 (44.1 3.0 (3.0 42. (42. 6. (6. 44. (44. 3. (3. (42 (6 (44 (3 (4 (

Fruits of Schinus terebinthifolia Raddi were investigated. A fast and efficient method for the simultaneous determination of 11 polyphenolic substances by high performance liquid chromatography-diode array detector (HPLC-DAD) was applied. Limits of quantification ranged between 0.78 and 3.14 mg L-1. High concentrations of kaempferol were found. An exploratory evaluation was performed using principal component analysis (PCA) for fruit origin differentiation. Total phenolic content (TPC) ranged from 274 to 517 mg GAE 100 g-1; total flavonoid content (TFC) ranged from 101 to 283 mg QE 100 g-1. In vitro analyses showed that antioxidant capacity with 2,2-diphenyl-1-picrylhydrazyl (DPPH) ranged from 1.7 to 7 µM DPPH 100 g-1. The bacteriostatic and bactericidal effect were also evaluated against: Bacillus subtilis, B. cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella choleraesuis, Candida albicans and C. glabrata, using broth microdilution assay. It can be inferred that S. terebinthifolia Raddi fruit has the potential to act as an ally in the search for new alternatives in the action against pathogens, and it can be used as a potential natural antioxidant and antimicrobial agent. Metals (Cu, Fe, Mg, Mn and Zn) were also determined by flame atomic absorption spectrometry (FAAS), with emphasis on high concentrations of Mg, Zn and Fe. investigated 1 chromatographydiode chromatography diode HPLCDAD HPLC DAD (HPLC-DAD applied 078 0 78 0.7 314 3 14 3.1 L1. L1 L 1. L-1 found PCA (PCA differentiation TPC (TPC 27 51 10 g1 g g-1 TFC (TFC 28 g1. 2,2diphenyl1picrylhydrazyl 22diphenyl1picrylhydrazyl diphenylpicrylhydrazyl 2,2 diphenyl picrylhydrazyl 2 (DPPH 17 subtilis B cereus aureus coli aeruginosa choleraesuis C glabrata assay S pathogens agent Cu, Cu (Cu Fe Mg FAAS, FAAS , (FAAS) 07 0. 31 3. L- 5 g- 2diphenyl1picrylhydrazyl 22 2, (FAAS

RESUMO: A mangueira apresenta elevado índice de abscisão de frutos, que é potencializado em regiões semiáridas devido ao estresse ambiental. A utilização de bioestimulantes e fitorreguladores tem demonstrado ser uma estratégia promissora para melhorar a fixação de frutos. Assim, o presente estudo foi realizado com objetivo de definir a melhor estratégia para a fixação e desenvolvimento dos frutos, com a aplicação de fitorreguladores e bioestimulante na mangueira ‘Keitt’, cultivada no Vale do São Francisco. Foram realizados dois experimentos. No experimento 1, foi utilizado o delineamento de blocos casualizados, com quatro tratamentos T1: controle, T2: ácido giberélico AG3 + ácido 2,4-diclorofenoxiacético (2,4-D), T3: AG3+2,4-D+Benziladenina, T4: Bioestimulante + CoMo® (fontes de cobalto e molibdênio). No experimento 2, o esquema fatorial (2 ×3 + 1), correspondendo a duas formas de fertilização de potássio (recomendada e ajustada), três concentrações de giberelina (0, 10 e 20 mg L-1) e um tratamento adicional (controle - fertilização de potássio recomendada e sem estratégia de fixação). As variáveis analisadas incluem o número relativo de frutos, o número de frutos para os mercados doméstico e estrangeiro, o potencial produtivo, a produção, a produtividade, o volume de frutos, a massa dos frutos e o rendimento da casca, polpa e semente. A aplicação de 10 mg L-1 AG3 + 10 mg L-1 2,4-D + 10 mg L-1 de Benziladenina em floração plena da mangueira ‘Keitt’, aumentou a fixação e a produção de frutos. Independentemente da fertilização, as aplicações sequenciais de 10 ou 20 mg L-1 de AG3, mostraram ser uma estratégia eficaz para a produção da mangueira ‘Keitt’. A fertilização potássica ajustada e a aplicação de 10 mg L-1 de AG3 favorece a produção de frutos de maior a massa e o volume. RESUMO ambiental Assim ‘Keitt, Keitt ‘Keitt , ‘Keitt’ Francisco experimentos 1 casualizados T1 T controle T2 AG 2,4diclorofenoxiacético 24diclorofenoxiacético diclorofenoxiacético 2,4 2 4 2,4D, 24D D (2,4-D) T3 AG3+2,4D+Benziladenina, AG324DBenziladenina AGDBenziladenina AG3+2,4 D+Benziladenina, AG3+2,4-D+Benziladenina T4 CoMo fontes molibdênio. molibdênio . molibdênio) ( 3 × 1) ajustada, ajustada) 0, 0 (0 L1 L fixação. fixação) estrangeiro produtivo produtividade casca semente L- 2,4D ‘Keitt. 4diclorofenoxiacético 24 4D (2,4-D AG3+2,4D+Benziladenina DBenziladenina AG324 AG3+2, D+Benziladenina AG32 AG3+2 AG3+

ABSTRACT: The mango tree presents a high level of fruit abscission, which is increased in semiarid regions due to environmental stress. The use of biostimulants and phytoregulators has shown to be a promising strategy to improve fruit setting. Thus, the present study defined the best strategy for setting and development of the fruit, with the application of phytoregulators and biostimulants on the ‘Keitt’ mango tree, cultivated in the São Francisco Valley. Two experiments were performed. In experiment 1, a randomized block design was used, with four treatments T1: control, T2: gibberellic acid (AG3) + 2,4-dichlorophenoxyacetic acid (2,4-D), T3: AG3 + 2,4-D+Benzyladenine, T4: Biostimulant + CoMo® (sources of cobalt and molybdenum). In experiment 2, the factorial scheme (2 ×3 + 1) distributed in randomized blocks was established, corresponding to two forms of potassium fertilization (recommended and adjusted), three gibberellin concentrations (0, 10 and 20 mg L-1) and additional treatment (control - recommended potassium fertilization and no setting strategy). The variables analyzed include the relative number of fruits, the number of fruits for domestic and foreign markets, productive potential, production, productivity, fruit volume, fruit mass, and the yield of peel, pulp and stone. The application of 10 mg L-1 AG3 + 10 mg L-1 2,4-D + 10 mg L-1 Benzyladenine in full flowering of the ‘Keitt’ mango tree, increased the setting and the production of the fruit. Regardless of fertilization, sequential applications at 10 or 20 mg L-1 of AG3 proved to be an effective strategy to produce ‘Keitt’ mango tree. Adjusted potassium fertilization and the application at 10 mg L-1 of AG3 promotes the production of fruits with greater mass and volume. ABSTRACT abscission stress Thus ‘Keitt Keitt Valley performed 1 used T1 T control T2 AG (AG3 2,4dichlorophenoxyacetic 24dichlorophenoxyacetic dichlorophenoxyacetic 2,4 2 4 2,4D, 24D D , (2,4-D) T3 2,4D+Benzyladenine, 24DBenzyladenine DBenzyladenine D+Benzyladenine, 2,4-D+Benzyladenine T4 CoMo sources molybdenum. molybdenum . molybdenum) ( 3 × established adjusted, adjusted adjusted) 0, 0 (0 L1 L strategy. strategy) markets potential productivity volume peel stone L- 2,4D (AG 4dichlorophenoxyacetic 24 4D (2,4-D 2,4D+Benzyladenine D+Benzyladenine

RESUMO: Visando a qualidade nutricional e funcional dos resíduos agroindustriais, o estudo teve como objetivo estabelecer a mistura ternária de farinhas elaboradas com resíduos de abacaxi, acerola e maracujá utilizando o delineamento simplex-centroide, assim como avaliar seus efeitos nas características físico-químicas e sensoriais em gelados comestíveis. Com o planejamento de misturas, nove componentes ternários das farinhas foram aplicados em formulações de gelados comestíveis para avaliação dos escores de aceitação para os atributos sabor, textura e impressão global, bem como os parâmetros físico-químicos (pH, acidez, sólidos solúveis e parâmetros de cor) e microbiológicos (coliformes termotolerantes a 45 ºC, Enterobacteriaceae e Salmonella). O delineamento simplex-centroide demonstrou que o modelo cúbico melhor prediz os resultados para os atributos sensoriais analisados. Os gráficos de superfície de resposta indicaram a área de maior aceitação nas formulações com o máximo percentual estabelecido para a farinha com os resíduos de acerola. O maior índice de aceitabilidade foi atribuído a mistura ternária constituída com 60%, 30% e 10% para as farinhas de abacaxi, maracujá e acerola, respectivamente. Os resultados indicaram que o sorbet enriquecido com a farinha composta pela mistura ternária é um produto com potencial para exploração, além de contribuir para a redução dos impactos ambientais gerados pelo grande e inadequado descarte de resíduos do processamento agroindustrial de frutas no ecossistema. RESUMO agroindustriais abacaxi simplexcentroide, simplexcentroide simplex centroide, centroide físicoquímicas físico químicas misturas sabor global físicoquímicos químicos pH, pH (pH acidez cor coliformes 4 ºC Salmonella. Salmonella . Salmonella) analisados 60 60% 30 10 respectivamente exploração ecossistema 6 3 1

ABSTRACT: To improve the nutritional and functional quality of agro-industrial waste, his study established a ternary mixture of flours made from pineapple, acerola, and passion fruit waste using the simplex-centroid design, as well as to evaluate their effects on the physicochemical and sensory characteristics of edible ice cream. Using mixture planning, nine ternary flour components were applied to edible ice cream formulations to evaluate acceptance scores for taste, texture, and overall impression attributes, as well as physicochemical parameters (pH, acidity, soluble solids, and color parameters) and microbiological parameters (thermotolerant coliforms at 45 ºC, Enterobacteriaceae and Salmonella). The simplex-centroid design demonstrated that the cubic model best predicts the results for the analyzed sensory attributes. The response surface graphs indicated the area of greatest acceptance in the formulations with the maximum percentage established for the flour with acerola waste. The highest level of acceptability was given to the ternary mixture of 60%, 30%, and 10% pineapple, passion fruit, and acerola flours, respectively. The results indicated that sorbet enriched with flour composed of the ternary mixture is a product with the potential for exploitation, as well as contributing to the reduction of environmental impacts generated by the large and improper disposal of agro-industrial fruit processing residues in the ecosystem. ABSTRACT agroindustrial agro industrial pineapple simplexcentroid simplex centroid planning taste texture attributes pH, pH (pH acidity solids thermotolerant 4 ºC Salmonella. Salmonella . Salmonella) 60 60% 30 30% 10 respectively exploitation ecosystem 6 3 1

RESUMO A ressonância magnética nuclear (RMN) é uma técnica de espectroscopia amplamente utilizada pelos químicos e físicos para determinar a estrutura química de moléculas que foi adaptada para gerar imagens, conhecidas como tomografia por imagem de ressonância magnética nuclear (IRM), amplamente utilizada em diagnóstico médico. A importância da RMN na química, física, medicina, materiais e agricultura tem sido reconhecida por vários Prémios Nobel de Física, 1952, Química, 1991 e 2002, e Medicina em 2003. Portanto, a RMN pode ser aplicada para obter: i) imagens do corpo humano, animais e materiais; ii) espectros de alta resolução para obtenção de informações estruturais e dinâmicas de produtos químicos, materiais etc.; e iii) obter informações quantitativas e qualitativas da composição química de alimentos, produtos agrícolas, utilizando relaxometria de baixa resolução. A IRM e a RMN de alta resolução tem sido aplicadas em produtos agroalimentares, principalmente na pesquisa, uma vez que normalmente dependem de instrumentos caros de grandes dimensões, o que restringe a sua utilização em aplicações rotineiras. Sensores de RMN que têm sido utilizados com mais frequência em produtos agroalimentares baseiam-se em instrumentos de baixa resolução ou de baixo campo ou domínio de tempo TD-NMR. Esses instrumentos de baixo custo tem sido utilizados para análises qualitativas e quantitativas de produtos agroalimentares, como sementes e grãos intactos, frutas intactas, carnes, óleos e alimentos processados. Neste artigo, é apresentada uma revisão das técnicas de RMN, seus principais aspectos da instrumentação, e discutidas algumas aplicações de TD-RMN e IRM na análise não invasiva de alimentos, sementes e outros produtos agrícolas. (RMN IRM, , (IRM) médico física medicina Física 1952 Química 199 2002 2003 Portanto i humano ii etc. etc iii agrícolas pesquisa dimensões rotineiras baseiamse baseiam se TDNMR. TDNMR TD NMR. NMR TD-NMR intactos intactas carnes processados artigo instrumentação TDRMN (IRM 195 19 200 1 20 2

ABSTRACT Nuclear magnetic resonance (NMR) is a spectroscopy technique widely used by chemists and physicists to determine the chemical structure of molecules that was adapted to generate imaging, known as nuclear magnetic resonance imaging (MRI), which is widely used in medical diagnosis. The importance of NMR in chemistry, physics, medicine, materials, and agriculture has been recognized with several Nobel Prizes in Physics, 1952, Chemistry, 1991 and 2002, and Medicine in 2003. Therefore, NMR can be applied to obtain: i) imaging of the human body, animal and materials; ii) high-resolution spectra to obtain structural and dynamical information of chemicals, materials etc.; and iii) quantitative and qualitative information of chemical composition of products such as food and agricultural products, using low-resolution relaxometry. High-resolution NMR and MRI have been applied in agri-food products, mostly as a research tool as they typically rely on expensive and bulk instruments, which restrict their uses in routine applications. The NMR sensors that have been more frequently used in agri-food products are based on low-resolution or low-field or time-domain NMR (TD-NMR) instruments. These low-cost instruments have been used for qualitative and quantitative analysis of agri-food products such as intact seeds and grains, intact fruits, meat, oils, and processed foods. In this paper, an overview of the NMR techniques and its main instrumentation aspects are presented, and some applications of TD-NMR and MRI in the non-invasive analysis of food, seeds, and others agricultural products are discussed. (NMR MRI, , (MRI) diagnosis chemistry physics medicine Physics 1952 Chemistry 199 2002 2003 Therefore i body ii highresolution high resolution chemicals etc. etc iii lowresolution low relaxometry Highresolution High agrifood agri lowfield field timedomain time domain TDNMR TD (TD-NMR lowcost cost grains fruits meat oils foods paper presented noninvasive non invasive discussed (MRI 195 19 200 1 20 2

Brazil is home to a rich biodiversity of native edible fruits renowned for their health-promoting compounds and flavors. Among these, Eugenia uniflora L. (Brazilian pitanga) is particularly notable. The fruit varies in color from yellow to purple and is prized for its intense aroma and versatile taste profile. However, information about the compounds responsible for their nutritional and sensory potential is still limited. This study analyzed volatile compounds and metabolites in yellow, red and purple pitanga fruits (Eugenia uniflora) at various ripening stages, exploiting a metabolomics approach. Yellow pitanga had 30 volatile compounds, with esters (40%), terpenes (26.67%) and aldehydes (16.67%) constituting 83.34% of identified volatile compounds. Red pitanga featured 26 volatile compounds, primarily terpenes and esters (each 30.77%) and aldehydes (15.38%), making up 76.92%. Purple pitanga contained 39 volatile compounds, with terpenes and esters (each 35.90%) and aldehydes (17.95%), totaling 89.75% of identified volatile compounds. Across all pitanga types, 124 metabolites were identified, including saturated fatty acids (17.74%), terpenes (17.74%) and sugars (16.93%), constituting 52.41% of identified metabolites. Future studies should provide nutritional information, consumption methods and industrial applications for pitanga fruits, focusing on sensory analysis, health benefits, preservation and breeding to enhance bioactive compounds and flavor. healthpromoting promoting flavors these L Brazilian notable profile However limited stages approach 3 40%, 40 40% , (40%) 26.67% 2667 67 (26.67% 16.67% 1667 16 (16.67% 8334 83 34 83.34 2 each 30.77% 3077 77 15.38%, 1538 15.38% 15 38 (15.38%) 7692 76 92 76.92% 35.90% 3590 35 90 17.95%, 1795 17.95% 17 95 (17.95%) 8975 89 75 89.75 types 12 17.74%, 1774 17.74% 74 (17.74% 16.93%, 1693 16.93% 93 (16.93%) 5241 52 41 52.41 analysis benefits flavor 4 (40% 26.67 266 6 (26.67 16.67 166 1 (16.67 833 8 83.3 30.77 307 7 153 15.38 (15.38% 769 9 76.92 35.90 359 179 17.95 (17.95% 897 89.7 177 17.74 (17.74 169 16.93 (16.93% 524 5 52.4 (40 26.6 (26.6 16.6 (16.6 83. 30.7 15.3 (15.38 76.9 35.9 17.9 (17.95 89. 17.7 (17.7 16.9 (16.93 52. (4 26. (26. 16. (16. 30. 15. (15.3 76. 35. 17. (17.9 (17. (16.9 ( (26 (16 (15. (17 (2 (1 (15

Abstract Introduction. In organic agriculture, green manures and biocontrols are used as methods for fertilization and pest control. However, their independent application of these inputs increases production costs. A pelletized formulation is proposed to allow simultaneous application of both inputs. Objective. To evaluate the effect of pelletized Mucuna pruriens (L.) DC. inoculated with Trichoderma harzianum Rifai on nutritional intake, agricultural yield, and regulation of Fusarium oxysporum f. sp. lycopersici in tomato (Solanum lycopersicum L.). Materials and methods. The experiment was carried out in 2022 in Tablón del Guarco, Cartago, Costa Rica, using a randomized complete block design with seven treatments and seven repetitions. Two formulations (A and B) of biopellet (M. pruriens + zeolite + T. harzianum) were applied at three dosages (15, 30, 50 g/plant). T. harzianum was added at a rate of 5 g/kg of biopellet. Applications of each treatment were conducted every 15 days for a total of eight applications. At the time of planting, F. oxysporum was inoculated (100 mL/plant with a concentration of 1 x 103 CFU). Results. The application of biopellet achieved a yield and weight of high-quality fruits similar to the commercial control. There were no significant differences among treatments for second and third-quality fruits. The technical efficiency in controlling F. oxysporum reached 97.6 % with biopellet. This amendment increased the levels of Carbon (C), Nitrogen (N), and organic matter in the soil and affected the nutritional content of the plant foliage in the first 60 days after sowing. Conclusions. The biopellet has the capacity to make chemical and organic contributions to the soil, which improves the nutrition of the tomato crop. It could be also a biocontrol alternative against F. oxysporum and compatible with T. harzianum.

Resumen Introducción. En agricultura orgánica, se utilizan abonos verdes y biocontroladores como métodos de fertilización y control de plagas. Sin embargo, la aplicación independiente de estos incrementa los costos de producción. Se propone una presentación peletizada que permita la aplicación conjunta de ambos insumos. Objetivo. Evaluar el efecto de Mucuna pruriens (L.) DC. peletizada e inoculada con Trichoderma harzianum Rifai en el aporte nutricional, el rendimiento agrícola y la regulación de Fusarium oxysporum f. sp. lycopersici en tomate (Solanum lycopersicum L.). Materiales y métodos. El experimento se ejecutó el año 2022 en Tablón del Guarco, Cartago, Costa Rica, bajo un diseño de bloques completos al azar con siete tratamientos y siete repeticiones. Se aplicaron dos formulaciones (A y B) de biopélet (M. pruriens + zeolita + T. harzianum) en tres dosificaciones (15, 30, 50 g/planta). La adición de T. harzianum se efectuó a razón de 5 g por cada kilo del biopélet. Se realizaron aplicaciones cada 15 días por tratamiento para un total de ocho aplicaciones. Se inoculó F. oxysporum (100 mL/planta con una concentración de 1 x 103 UFC) a la siembra. Resultados. La aplicación del biopélet mostró un rendimiento y peso de frutos de primera calidad similar al testigo comercial. En frutos de segunda y tercera calidad, no hubo diferencias significativas entre tratamientos. La eficiencia en el control de F. oxysporum alcanzó el 97,6 % con biopélet. Esta enmienda aumentó los niveles de Carbono, Nitrógeno y materia orgánica en el suelo e influenció el contenido nutricional en el follaje a los 60 días después de la siembra. Conclusiones. El biopélet tiene la capacidad de realizar aportes químicos y orgánicos al suelo, por lo cual mejora la nutrición del cultivo del tomate. Los resultados sugieren además que es una alternativa de biocontrol contra F. oxysporum y compatible con T. harzianum.

Abstract Introduction. “Green mold,” caused by Penicillium digitatum is one of the phytosanitary issues that limits citrus production. For its control, synthetic fungicides are used, which have lost effectiveness due to resistance developed by the fungus. An alternative is the use of endophytic fungi with biocontrol action. Objective. To evaluate the effect of secondary metabolites (SM) from endophytic fungi (EF) on the mycelial inhibition of Penicillium digitatum and the effectiveness of disease control in mandarin fruits (Citrus reticulata) post-harvest. Materials and methods. The study was conducted in 2022, using a completely randomized experimental design in the Plant Health Laboratory at the Universidad Nacional de Cañete (UNDC), Lima, Peru. For the antibiosis test, secondary metabolites were extracted from ten strains of EF from the genera Trichoderma sp., Fusarium sp., and Aspergillus sp., where mycelial inhibition of the pathogen (%) was evaluated. SMs were sprayed at 15 % (v/v) concentration on mandarin fruits that were disinfected and inoculated with the pathogen by spraying (1x 105 CFU/cc). Additionally, a commercial biocontrol, a vegetable oil, and a fungicide were used. After 6 and 9 days, disease incidence (%) and severity index (0, 1, 2, 3) were evaluated. Results. Secondary metabolites from strains the HEA-111 (Aspergillus), HEA-109 (Fusarium), and HSA-1 (Trichoderma) showed the highest mycelial inhibition with 95.05 %, 87.51 %, and 47.47 %, respectively. In mandarin fruits treated with SMs from strains HEA-109 and HSA-1, there was a control of 80 % and 55 %, respectively. Conclusions. SMs from EF were effective in inhibiting the mycelial growth of P. digitatum and reduces disease progression in mandarin fruits post-harvest.

Resumen Introducción. El “moho verde” causado por Penicillium digitatum es uno de los problemas fitosanitarios que limita la producción en los cítricos. Para su control se utilizan fungicidas sintéticos, que han perdido eficacia, debido a la resistencia generada por el hongo. Una alternativa, es el uso de hongos endófitos con acción biocontroladora. Objetivo. Evaluar el efecto de metabolitos secundarios (MS) de hongos endófitos (HE) en la inhibición micelial de Penicillium digitatum y la eficacia de control de la enfermedad, en frutos de mandarina (Citrus reticulata) después de la cosecha. Materiales y métodos. El estudio se realizó en 2022, bajo un diseño experimental completo al azar, en el laboratorio de Sanidad Vegetal, Universidad Nacional de Cañete (UNDC), Lima, Perú. Para la prueba de antibiosis se extrajeron los metabolitos secundarios de diez cepas de HE de los géneros Trichoderma sp., Fusarium sp. y Aspergillus sp., en donde se evaluó la inhibición micelial del patógeno (%). Se asperjaron MS a una concentración del 15 % (v/v) sobre frutos de mandarina que fueron desinfectados e inoculados con el patógeno por aspersión (1x105 UFC/cc). Adicionalmente, se utilizaron un biocontrol comercial, un aceite vegetal y un fungicida. Después de 6 y 9 días, se evaluaron la incidencia (%) y el índice de severidad (0,1,2,3) de la enfermedad. Resultados. Los metabolitos secundarios de las cepas HEA-111 (Aspergillus), HEA-109 (Fusarium) y HSA-1 (Trichoderma) presentaron la mayor inhibición micelial con 95,05 %, 87,51 % y 47,47 %, respectivamente. En los frutos de mandarina tratados con los MS de las cepas HEA-109 y HSA-1, hubo un control del 80 % y 55 %, respectivamente. Conclusiones. Los MS de HE tuvieron efecto en la inhibición micelial de P. digitatum y redujeron el avance de la enfermedad en frutos de mandarina después de la cosecha.

Abstract Introduction. The cultivation of dragon fruit (Hylocereus costaricensis and H. monocanthus) is increasing in Costa Rica, but technical information for its management, such as nutritional requirements, fertilization timing, and growth behavior, is lacking. Objective. To quantify and model the growth and nutrient uptake of dragon fruit (H. costaricensis and H. monocanthus) under dry tropics conditions in Costa Rica. Materials and methods. The research was conducted from 2017 to 2020 in Guanacaste, Costa Rica. Plants were sampled at 76, 165, 308, 450, 607, 903, 1249, and 1706 days after planting (DAP) and 205 fruits were analyzed. Dry matter was quantified, and the uptake of N, P, Ca, Mg, K, S, Fe, Cu, Zn, Mn, and B was calculated. Two logistic models were fitted to determine nutrient uptake, and linear regressions were calibrated for fresh fruit. Results. For a planting density of 3000 plants/ha, the nutritional requirements per hectare were: 137 kg N, 32 kg P, 327 kg K, 63 kg Mg, 32 kg S, 1,60 kg Zn, and 0,21 kg B. The best-fitting logistic model (RMSE = 0.09) accurately estimated the uptake of N, P, Ca, S, Zn, and B. The linear regression model demonstrated high predictive capacity (R2 > 0.85) for the nutrient requirements of N, P, K, Mg, and S in the fruit. Conclusion. Logistic models provided high precision in deterministically modeling nutrient uptake in dragon fruit plants. The study affectively quantified and modeled the nutritional behavior of the plants under Costa Rica’s dry tropical conditions, providing essential information for optimizing fertilization programs for the crop.

Resumen Introducción. El cultivo de pitahaya (Hylocereus costaricensis y H. monocanthus) está en aumento en Costa Rica, pero carece de información técnica para su manejo, como necesidades nutricionales, momentos de fertilización y comportamiento del crecimiento. Objetivo. Cuantificar y modelar el crecimiento y la absorción de nutrimentos del cultivo de pitahaya (H. costaricensis y H. monocanthus) en condiciones del trópico estacional seco de Costa Rica. Materiales y métodos. La investigación se realizó durante los años 2017-2020, en Guanacaste, Costa Rica. Se muestrearon plantas a los 76, 165, 308, 450, 607, 903, 1249 y 1706 días después de siembra (DDS) y 205 frutas. Se cuantificó la materia seca y se calculó la absorción de N, P, Ca, Mg, K, S, Fe, Cu, Zn, Mn y B. Se ajustaron dos modelos logísticos para determinar la absorción de nutrientes y se calibraron regresiones lineales en fruta fresca. Resultados. Para una densidad de siembra de 3000 planta/ha los requerimientos nutricionales del cultivo por hectárea fueron: 137 kg N, 32 kg P, 327 kg K, 63 kg Mg, 32 kg S, 1,60 kg Zn y 0,21 kg B. El modelo logístico de mejor ajuste (RMSE = 0,09) estimó de forma precisa la absorción de N, P, Ca, S, Zn y B. El modelo de regresión lineal tuvo alta capacidad predictora (R2 > 0,85) para los requerimientos de N, P, K, Mg y S en fruta. Conclusión. Los modelos logísticos tuvieron alta precisión para modelar de manera determinística la absorción de nutrientes en plantas de pitahaya. Además, se logró cuantificar y modelar de forma eficiente el comportamiento nutricional de las plantas en condiciones de trópico estacional seco de Costa Rica, lo que proporciona información esencial para los programas de fertilización del cultivo.