ABSTRACT Mantle cell lymphoma of the ocular and periorbital regions is extremely rare but should be considered in the differential diagnosis of lesions affecting the periorbital tissues. In this study, we present a rare case of mantle cell lymphoma of the lacrimal sac in a 65-year-old male presenting with a mass in the lacrimal sac region and epiphora. After clinical examinations and imaging studies, the mucocele was misdiagnosed. Considering the unexpected findings during external dacryocystorhinostomy, a frozen biopsy was performed, which confirmed the diagnosis of lymphoma. tissues study 65yearold yearold 65 year old epiphora studies misdiagnosed dacryocystorhinostomy performed 6

Drug poisoning is an important area of study in South Africa as a treatable cause of mortality. While research has been conducted on poisoning, there is a paucity of literature on the availability of antidotes in South Africa. The objectives of this study were to assess the availability of antidotes in selected teaching hospitals in the Southern Gauteng City-Region and to explore doctors' experiences of antidote supply. The availability of antidotes in the emergency departments (EDs) and pharmacies was assessed and recorded using a data sheet that was completed in person at each of the teaching hospitals. A questionnaire exploring experiences of antidote supply was distributed to 126 doctors working in the EDs. Our results indicate that N-acetylcysteine, atropine, diazepam, clonazepam, sodium bicarbonate, vitamin K, calcium gluconate, naloxone, ethanol, and pyridoxine were present in all EDs; activated charcoal was present in 80%; lorazepam, glycopyrrolate, and calcium chloride in 60%; freeze-dried plasma in 40%; glucagon and desferrioxamine in 20%; and fresh frozen plasma, hydroxocobalamin, sodium nitrite, sodium thiosulfate, sodium calcium edetate, and intralipid were not present in any of the EDs. Doctors reported organophosphate poisoning and paracetamol overdose as the most common drug poisonings (81.7% and 14.3% of 126 respondents, respectively). Most doctors experienced no supply issues for N-acetylcysteine, calcium gluconate, sodium bicarbonate, or pyridoxine (85.7%, 83.3%, 87.3%, and 75.4% of 126 respondents, respectively). The antidotes to the most common poisonings reported by doctors were present in all EDs. However, concerns were raised about consistency of supply, which will be an important avenue for further research. SIGNIFICANCE: • These findings highlight the lack of uniform availability of antidotes to common and critical drug poisonings in emergency departments. • The experiences of doctors in Gauteng concerning the most commonly encountered poisonings contrast with existing literature from South Africa, suggesting regional differences within the country.

Abstract Introduction : Knee osteochondral lesions represent a frequent pathology within young active patients. One possible indication for severe lesions or in case of im possibility of harvesting an autograft is the use of fresh frozen allograft. The objective of this study was to ret rospectively analyze functional results and failure rate after osteochondral transplants using fresh frozen al lografts. Methods : We analyzed data from patients who under went knee osteochondral transplant using mosaicplasty technique with fresh frozen allografts at our institution between 2014 and 2019. We included those patients with at least two-year follow-up. Demographic characteristics such as age at the moment of intervention and size of the defect were included. Functional results were assessed using pre and postoperative Lysholm and IKDC scores. Patients who underwent a knee replacement were considered failures. Results : Twenty-five patients were included. The me dian age was 43.5 years (IQR 29-50), 45% were female and the mean follow-up was 83 months (SD 54.6). Mean osteochondral defect size was 4 cm2. Mean pre and post operative Lysholm scores were 39 (SD 19.3) and 82 (SD 15.4) respectively (p < 0.01). Mean pre and postoperative IKDC scores were 42 (SD 13.8) and 60 (SD 13.5) respec tively (p < 0.01). Five patients (20%) underwent a knee replacement afterwards and were considered failures. Discussion : Our results after a mean seven-year follow-up evidenced an overall improvement in func tional scores and a failure rate of 20%. Osteochondral transplant using fresh frozen allografts is a reliable and feasible treatment for patients with large osteochondral defects.

Resumen Introducción : Las lesiones osteocondrales de rodilla son una afección frecuente en jóvenes. Los trasplantes alogénicos usando injerto congelado se presentan como una opción de tratamiento en pacientes con lesiones grandes o sin zona dadora. Este trabajo buscó analizar retrospectivamente los resultados funcionales y la tasa de falla de los trasplantes osteocondrales con injerto cadavérico congelado. Métodos : Se incluyeron pacientes sometidos a tras plantes osteocondrales de rodilla con injerto cadavérico congelado en nuestra institución, entre 2014 y 2019, con dos años de seguimiento mínimo. Variables evaluadas: edad al momento de la intervención, escalas funciona les International Knee Documentation Committee (IKDC) y Lysholm pre y post operatorios, complicaciones y tasa de falla. Resultados : Incluimos 25 pacientes. La edad media fue de 43.5 años (RIQ 29-50), 45% fueron mujeres y el seguimiento promedio fue de 83 meses (DS 54.6). El ta maño promedio del defecto osteocondral fue de 4 cm2. La escala de Lysholm promedio pre y postoperatorio fue de 39 (DS 19.3) y 82 (DS 15.4) respectivamente (p < 0.01). El IKDC promedio pre y postoperatorio fue de 42 (DS 13.8) y 60 (DS 13.5) respectivamente (p < 0.01). La tasa de falla fue del 20% (n=5). Discusión : Los pacientes presentaron una mejoría postoperatoria evidenciada en los resultados funcio nales, y una tasa de falla del 20%. El uso de trasplante osteocondral congelado se presenta como un recurso útil para el tratamiento de lesiones condrales graves.

Abstract The present work is at the crossroads of territorial studies and a line of ethnographic research that analyses ethnographic research that analyses rubble as material traces of the failure of development projects. The policy of agricultural “diferimientos” in the province of San Juan provoked a series of territorial transformations that resulted in the arrival of numerous agricultural companies in the rural space of the province, but also the speculative use of these benefits, with the consequent abandonment of productive enterprises. These productive abandonments are understood in this paper in the light of the approach of Gastón Gordillo's to rubble as materiality or violence frozen in it. Beyond the interest that has been paid to the impacts of the “diferimientos” policy in the province of San Juan, the local effects they had have been neglected. This has led to a lack of understanding of the territorial dynamics that were awakened by the public policy. The presence of rubble symbolises the paradoxical nature of the “diferimientos” policy process. This performance reflects the disconnection between implemented policies and local realities, thus calling for a profound reconsideration of rural development strategies.

Resumen El presente trabajo se encuentra en el cruce de los estudios territoriales y de una línea de investigación etnográfica que analiza los escombros como huellas materiales del fracaso de los proyectos del desarrollo. La política de diferimientos agrícolas en la provincia de San Juan provocó una serie de transformaciones territoriales que tuvieron por resultado el arribo de numerosas empresas agrícolas al espacio rural de la provincia, pero también la utilización especulativa de estos beneficios con el consiguiente abandono de emprendimientos productivos. Estos abandonos productivos son entendidos en este trabajo a la luz del abordaje de Gastón Gordillo referido a los escombros en tanto materialidad o violencia congelada en ellos. Más allá del interés que se les ha prestado a los impactos de la política de diferimiento en la provincia de San Juan se han desatendido los efectos locales que ellos tuvieron. Lo cual ha ocasionado una desatención a las dinámicas territoriales que se despertaron a partir de la política pública. La presencia de los escombros simboliza lo paradojal del proceso de la política de diferimiento. Este desempeño refleja la desconexión entre las políticas implementadas y las realidades locales, por tanto, exige una reconsideración profunda de las estrategias de desarrollo rural.

ABSTRACT Dengue is a viral infection which is transmitted by the Aedes aegypti mosquito and has four serotypes (DENV-1 to DENV-4). The disease triggers a variety of clinical manifestations, ranging from mild forms without warning signs to severe lifethreatening forms. We present the case of a 5-year-old boy, from the province of Callao, whose first symptoms were fever, headache and general malaise. On the third day, the child had mild abdominal pain and little vomiting; subsequently, abdominal distension, jaundice and choluria. He was admitted to the pediatric intensive care unit being alert and with moderate dehydration, jaundice, edema, distended and tender abdomen, shifting dullness and liver 2 cm below the right costal margin. Complementary tests revealed liver failure, hepatosplenomegaly and pleural effusion in the bases. Using a reactive IgM ELISA, severe dengue was diagnosed, as well as a superinfection due to probable spontaneous bacterial peritonitis. He started treatment with antibiotics, furosemide, fresh frozen plasma, cryoprecipitate and metamizole. As the child did not get better, the diuretic was optimized, and human albumin was administered. Thereafter, he got better showing decreased ascites, edema, jaundice and pleural effusion; improvement of the liver and coagulation profile; and being afebrile. He unexpectedly presented respiratory distress due to congestive heart failure caused by dilated cardiomyopathy diagnosed by echocardiography; thus, he was treated with diuretics. The patient was discharged afebrile, without edema and with resolution of liver failure and coagulation disorder.

RESUMEN El dengue es una infección viral transmitida a través del mosquito Aedes aegypti y presenta cuatro serotipos (DENV-1 a DENV-4). La enfermedad desencadena una variedad de manifestaciones clínicas, desde formas leves sin signos de alarma hasta formas graves, potencialmente mortales. Se presenta el caso de un niño de cinco años, procedente de la provincia del Callao, cuyos síntomas iniciales fueron fiebre, cefalea y malestar general. Al tercer día, el niño manifiestó dolor abdominal leve y vómitos escasos; posteriormente, distensión abdominal, ictericia y coluria. Fue hospitalizado en la unidad de cuidados intensivos pediátricos con deshidratación moderada, ictericia, edemas, abdomen distendido y doloroso, matidez desplazable, hígado a 2 cm debajo del reborde costal derecho y lúcido. Por exámenes complementarios, se evidenció falla hepática, hepatoesplenomegalia y derrame pleural en bases. Se diagnosticó dengue grave a través de una prueba de ELISA Ig M reactivo más sobreinfección por probable peritonitis bacteriana espontánea. Se inició el tratamiento con antibióticos, furosemida, plasma fresco congelado, crioprecipitado y metamizol. Al no observarse mejoría, se optimizó el diurético y se administró albúmina humana. Mostró mejoría con disminución de ascitis, edemas, ictericia y efusión pleural; también mejora del perfil hepático y de la coagulación, además de encontrarse afebril. Presentó inesperadamente dificultad respiratoria por insuficiencia cardiaca congestiva debido a miocardiopatía dilatada según ecocardiografía; se manejó con diuréticos. Fue dado de alta en estado afebril, sin edemas y con resolución de falla hepática y trastorno de coagulación.

Abstract To solve the problem that the mechanical behavior of undisturbed loess in seasonally frozen soil area is affected by freeze-thaw action, triaxial shear tests of undisturbed loess under freeze-thaw condition were carried out. The results show that the mechanical properties of undisturbed loess are greatly affected by factors including freeze-thaw process, water content, natural density and confining pressure. Freeze-thaw action has a certain impact on the failure surface shape and stress-strain curve. Before and after freeze-thaw, the shape of the shear failure surface is complex, including single oblique failure surface, double oblique failure surface, vertical failure surface, X-shaped failure surface, bulging failure, etc. And under the conditions of low water content, low confining pressure and high dry density, the stress-strain curve tends to be softened. Conversely, the curve tends to harden. Freeze-thaw action can make the stress-strain curve transition from softening to hardening. In addition, the freeze-thaw action significantly weakens the failure strength, shear strength, cohesion, initial tangent modulus and failure ratio of undisturbed soil, but does not change the internal friction angle obviously. Also, the heterogeneity of natural soil is also an important factor affecting the mechanical parameters, failure surface shape and stress-strain curve of undisturbed loess. freezethaw freeze thaw out process content Freezethaw Freeze stressstrain stress strain freezethaw, thaw, complex Xshaped X shaped etc softened Conversely harden hardening addition strength cohesion obviously Also parameters

ABSTRACT In this article, we present the case of a 47-year-old man who underwent Bentall-Bono procedure and frozen elephant trunk prosthesis implantation due to severe aortic regurgitation and aortic dilatation with a second-time endovascular stent-graft repair in descending aorta. Over eight years, a subacute graft infection by Propionibacterium acnes was developed, culminating in cardiogenic shock secondary to severe aortic regurgitation due to a complete aortic root dehiscence because of multiple aortic pseudoaneurysms. The patient underwent emergency surgery in which the replacement of the graft by a biological valve tube was performed accompanied by a complete debranching of the three supra-aortic vessels. article 47yearold yearold 47 year old BentallBono Bentall Bono secondtime second time stentgraft stent aorta years developed pseudoaneurysms supraaortic supra vessels 4

Abstract This study aimed to analyze the characteristics of the HSP70 gene and protein in spermatozoa of Bali bulls of different age groups and to examine its potential as a biomarker determining bull fertility. This study used frozen semen produced from six Bali bulls divided into two groups based on age (≤ 9 years and ≥ 12 years). Parameters of frozen semen quality analyzed included sperm motility and kinetics using computer-assisted semen analysis, sperm morphological defects using Diff-Quick staining, acrosome integrity using FITC-PNA staining, and DNA fragmentation using acridine orange staining. HSP70 gene expression characterization was analyzed using qRT-PCR, and HSP70 protein abundance was analyzed using enzyme immunoassays. Fertility field data were obtained by analyzing the percentage conception rate for each bull based on the artificial insemination service data contained in the Indonesian-integrated system of the National Animal Health Information System (iSIKHNAS). The results showed significant differences (P<0.05) in total and progressive motility, morphological defects of the neck and midpiece, and tail of sperm, and acrosome integrity between the age groups of Bali bulls. HSP70 gene expression and protein abundance showed no significant differences (P>0.05) in different age groups. HSP70 gene expression correlated with fertility rate (P<0.05). Age affected several semen quality parameters but did not affect HSP70 gene expression and protein abundance. The HSP70 gene molecule could be a biomarker that determines the fertility of Bali bulls. HSP HSP7 ≤ ( 1 years. . years) computerassisted computer assisted analysis DiffQuick Diff Quick staining FITCPNA FITC PNA qRTPCR, qRTPCR qRT PCR, PCR qRT-PCR immunoassays Indonesianintegrated Indonesian integrated iSIKHNAS. iSIKHNAS (iSIKHNAS) P<0.05 P005 P 0 05 (P<0.05 midpiece P>0.05 (P>0.05 P<0.05. (iSIKHNAS P<0.0 P00 (P<0.0 P>0.0 (P>0.0 P<0. P0 (P<0. P>0. (P>0. P<0 (P<0 P>0 (P>0 P< (P< P> (P> (P

ABSTRACT Ligneous conjunctivitis is a rare chronic form of recurrent membranous inflammation and plasminogen deficiency. Ocular manifestations may be associated with sites other than mucous membranes, such as the oral cavity, internal ear, respiratory, genitals, and kidney. Treatment is extremely difficult because of the lack of topic plasminogen drops, and a high volume is required for systemic supplementation. This report aimed to present two patients with ligneous conjunctivitis treated with membrane excision, topical fresh-frozen plasma, and heparin intra-, and postoperatively. No recurrence was found in the ligneous membrane in the 12-month follow-up. The use of topical fresh-frozen plasma and heparin after membrane excision could be effective to avoid recurrence. deficiency membranes cavity ear respiratory genitals kidney drops supplementation freshfrozen fresh frozen intra, intra , intra- postoperatively 12month month 12 followup. followup follow up. up follow-up 1

ABSTRACT Ligneous conjunctivitis is a rare chronic form of recurrent membranous inflammation and plasminogen deficiency. Ocular manifestations may be associated with sites other than mucous membranes, such as the oral cavity, internal ear, respiratory, genitals, and kidney. Treatment is extremely difficult because of the lack of topic plasminogen drops, and a high volume is required for systemic supplementation. This report aimed to present two patients with ligneous conjunctivitis treated with membrane excision, topical fresh-frozen plasma, and heparin intra-, and postoperatively. No recurrence was found in the ligneous membrane in the 12-month follow-up. The use of topical fresh-frozen plasma and heparin after membrane excision could be effective to avoid recurrence. deficiency membranes cavity ear respiratory genitals kidney drops supplementation freshfrozen fresh frozen intra, intra , intra- postoperatively 12month month 12 followup. followup follow up. up follow-up 1

■ ABSTRACT Introduction: Fat grafting is an autologous graft of cells from subcutaneous tissue, which can be used as a complementary technique in breast reconstruction. Given this, the cryopreservation of adipose tissue-derived mesenchymal stem cells (ADMSCs) could be a way to collect them in one surgical procedure and after performing fractional fat grafting. Dimethyl sulfoxide (DMSO) is a cryopreservative used in cell research, but it is potentially toxic, which would make it impossible to use cryopreserved ADMSCs in clinical practice. New cellular cryopreservatives, without toxicity, have been described in the experimental scientific literature, such as the substances L-proline and trehalose. Therefore, this work aimed to evaluate the viability of ADMSCs cryopreserved with the combination of L-proline and trehalose over up to 90 days. Method: Experimental study in which lipoaspirate samples were obtained from 9 patients. The cellular fraction was processed and frozen with L-proline (1.5M) + trehalose (0.2M) or with DMSO + fetal bovine serum (FBS) as control. After 30 and 90 days, the samples were thawed, and cell viability was assessed using the MTT technique. Results: The analysis of ADMSCs, after 1 and 3 months of freezing, indicated that samples treated with L-proline + trehalose showed similar viability to those preserved with DMSO and SFB (p=0.444). Conclusion: The association of L-proline and trehalose kept ADMSC viable for 30 and 90 days of freezing, and could be an alternative as a cellular cryopreservative without toxicity and enabling the use of serial fat grafting. Introduction tissue reconstruction tissuederived derived (ADMSCs (DMSO research toxic practice cryopreservatives literature Lproline L proline Therefore Method patients 1.5M 15M M 5M (1.5M 0.2M 02M 0 2M (0.2M FBS (FBS control thawed Results freezing p=0.444. p0444 p p=0.444 . 444 (p=0.444) Conclusion p044 p=0.44 44 (p=0.444 p04 p=0.4 4 (p=0.44 p0 p=0. (p=0.4 p=0 (p=0. p= (p=0 (p= (p

■ RESUMO Introdução: A lipoenxertia é um enxerto autólogo de células do tecido celular subcutâneo, que pode ser utilizada como técnica complementar na reconstrução mamária. Diante disso, a criopreservação de células-tronco mesenquimais provenientes de tecido adiposo (CTDAs) poderia ser uma maneira de realizar a coleta em um tempo cirúrgico e após realizar a lipoenxertia de forma fracionada. O dimetilsulfóxido (DMSO) é um criopreservante utilizado em pesquisas com células, porém é potencialmente tóxico, o que impossibilitaria a utilização de CTDAs criopreservadas na prática clínica. Novos criopreservantes celulares, sem toxicidade, vêm sendo descritos na literatura científica experimental, como as substâncias L-prolina e trealose. Com isso, esse trabalho teve como objetivo avaliar a viabilidade de CTDAs criopreservadas com a combinação de L-prolina e trealose, em um período de até 90 dias. Método: Estudo experimental, no qual foram obtidas amostras de lipoaspirado provenientes de 9 pacientes. A fração celular foi processada e congelada com L-prolina (1,5M) + trealose (0,2M), ou com DMSO + soro fetal bovino (SFB), como controle. Após 30 e 90 dias, as amostras foram descongeladas e a viabilidade celular foi avaliada pela técnica de MTT. Resultados: A análise das CTDAs, após 1 e 3 meses de congelamento, indicou que as amostras tratadas com L-prolina + trealose apresentaram viabilidade semelhante àquelas preservadas com DMSO e SFB (p=0,444). Conclusão: A associação de L-prolina e trealose manteve CTDA viáveis por 30 e 90 dias de congelamento, podendo ser uma alternativa como criopreservante celular sem toxicidade e viabilizando o uso de lipoenxertia seriada. Introdução subcutâneo mamária disso célulastronco tronco (CTDAs fracionada (DMSO tóxico clínica celulares experimental Lprolina L prolina isso Método pacientes 1,5M 15M M 5M (1,5M 0,2M, 02M 0,2M , 0 2M (0,2M) SFB, (SFB) controle MTT Resultados congelamento p=0,444. p0444 p p=0,444 . 444 (p=0,444) Conclusão seriada (0,2M (SFB p044 p=0,44 44 (p=0,444 p04 p=0,4 4 (p=0,44 p0 p=0, (p=0,4 p=0 (p=0, p= (p=0 (p= (p

Abstract Roadkill is one of the most direct impacts on fauna, leading to numerous direct and indirect impacts. Road-killed animals could serve as a resource for research and teaching purposes, but first, they need to have their tissues preserved by some technique. Plastination is one of the most modern techniques for the conservation of biological tissues, its principle is the replacement of body fluids by a curable polymer, resulting in a non-toxic, odorless, and manipulable material. So the main aim of this work was to compose a collection of plastinated roadkill victims from the Biological Reserve Sooretama region, adapting the standard protocol to the different taxa worked here (Avialae, Reptilia, Amphibia, and Mammalia). For this, 42 animals were used, previously frozen and stored in the Sooretama Biological Reserve and Vale Natural Reserve until they were sent for processing. Upon arriving at the Plastination laboratory at the Federal University of Espírito Santo, the animals underwent the 4 stages of plastination: fixation, dehydration, forced impregnation, and chemical cure; some adaptations were made to improve the final specimens. The results proved that the plastination of these animals is extremely viable in generating satisfactory results. Given the results obtained, it becomes clear how plastination can become an alternative to implement in the composition of biological collections using roadkill animals. The animals prepared in this work comprise an unprecedented collection available for different applications, such as exhibitions, practical classes, and research. fauna Roadkilled Road killed purposes first technique polymer nontoxic, nontoxic non toxic, toxic non-toxic odorless material region Avialae, Avialae (Avialae Reptilia Amphibia Mammalia. Mammalia . Mammalia) used processing Santo fixation dehydration impregnation cure specimens obtained applications exhibitions classes

Abstract Based on the theory of solid porous media with pores, the dynamic response of layered saturated frozen soil under uniform moving load is studied. Firstly, the governing equation of saturated frozen soil is established. Then, the Fourier integral transform is used to decouple the governing equation of saturated frozen soil, and the general solution of the potential function is obtained, and the corresponding force and displacement of each layer of saturated frozen soil are derived. Finally, using the transfer matrix method, combined with the continuous condition, the semi-analytical solution of the layered saturated frozen soil medium in the frequency domain under the surface permeable condition is derived. After verifying the accuracy of the solution by comparing the model degradation with the existing literature, the effects of soil shear modulus, load moving speed, temperature, contact parameter and frequency on the dynamic response are analyzed in detail. The results show that the order of soft and hard soil layers is of great significance to accurately evaluate the dynamic response of saturated frozen soil. pores studied Firstly established Then obtained derived Finally method semianalytical semi analytical literature modulus speed temperature detail

Abstract This study aimed to evaluate the efficiency of two or three estrus synchronizations followed by artificial insemination (AI) by laparoscopy with frozen semen in sheep. Santa Inês sheep (n=147) were randomly distributed into two groups: Re-sync - 2FTAI (n=72) and Doppler - 3FTAI (n=75), synchronized with a short protocol associated with GnRH and AI by laparoscopy with frozen semen 50 h after removing the P4 device (D0). Two AIs were performed in the Re-sync group, with the start of the resynchronization protocol on D23 and gestational diagnosis on D30; the non-pregnant females were inseminated again. The sheep were resynchronized (D10) in the Doppler group and the gestation diagnosis was made early at 17 days using Doppler ultrasound. The open females were inseminated again, totaling three inseminations in 42 days. Early gestation diagnoses were confirmed at 30 days. Re-sync (29.16%) and Doppler (21.33%) were similar to each other in terms of cumulative gestation rate (P>0.05). The indicators of the non-gestation diagnosis technique at 17 days were sensitivity of 100%, specificity of 28%, positive predictive value of 21.3%, negative predictive value of 100%, and accuracy of 39.8%. Early diagnosis allowed sheep to be inseminated two to three times in the same period and was effective in diagnosing truly non-pregnant females. Therefore, estrus resynchronization associated with AI by laparoscopy using frozen semen can be applied in sheep, but a higher gestation rate could not be achieved with two or three inseminations at the end of the breeding season. (AI n=147 n147 n 147 (n=147 groups Resync Re sync FTAI n=72 n72 72 (n=72 n=75, n75 n=75 , 75 (n=75) 5 P D0. D0 D . (D0) D2 D30 nonpregnant non pregnant again D10 (D10 1 ultrasound 4 3 29.16% 2916 29 16 (29.16% 21.33% 2133 21 33 (21.33% P>0.05. P005 P>0.05 0 05 (P>0.05) nongestation 100 100% 28 28% 213 21.3% 398 39 8 39.8% Therefore season n=14 n14 14 (n=14 n=7 n7 7 (n=7 (n=75 (D0 D3 D1 (D1 29.16 291 2 (29.16 21.33 (21.33 P00 P>0.0 (P>0.05 10 21.3 39.8 n=1 n1 (n=1 n= (n= (D 29.1 (29.1 (21.3 P0 P>0. (P>0.0 21. 39. (n 29. (29. (21. P>0 (P>0. (29 (21 P> (P>0 (2 (P> ( (P

Resumo Objetivou-se avaliar a eficiência de duas ou três sincronizações de estro seguida da inseminação artificial (IA) por laparoscopia com sêmen congelado em ovinos. As ovelhas Santa Inês (n=147) foram distribuídas aleatoriamente em 2 grupos: Re-sync - 2IATF (n=72) e Doppler - 3IATF (n=75), sincronizadas com protocolo curto associado ao GnRH e IA por laparoscopia com sêmen congelado 50 horas após a retirada do dispositivo P4 (D0). No grupo Re-sync foram realizadas duas IA com início do protocolo de ressincronização em D23 e diagnóstico gestacional em D30, as fêmeas não gestantes eram inseminadas novamente. No grupo Doppler as ovelhas foram ressincronizadas (D10) e o diagnóstico de gestação realizado precocemente aos 17 dias, com auxílio da ultrassonografa Doppler. As fêmeas vazias eram inseminadas novamente, totalizando três inseminações em 42 dias. Os diagnósticos precoces de gestação foram confirmados com 30 dias. Re-sync (29,16%) e Doppler (21,33%), foram semelhantes entre si na taxa de prenhez acumulativa (P>0,05). Os indicadores da técnica de diagnóstico de não-gestação aos 17 dias foram: sensibilidade 100%, especificidade 28%, valor preditivo positivo 21,3%, valor preditivo negativo 100% e acurácia 39,8%. Com o auxílio do diagnóstico precoce as ovelhas puderam ser inseminadas de duas a três vezes no mesmo período e mostrou efetividade em diagnosticar as fêmeas verdadeiramente não gestante. Conclui-se que a ressincronização de estro associada a IA por laparoscopia utilizando sêmen congelado pode ser aplicada em ovinos, entretanto não foi possível alcançar maior taxa de prenhez com duas ou três inseminações ao final da estação de monta. Objetivouse Objetivou se (IA ovinos n=147 n147 n 147 (n=147 grupos Resync Re sync IATF n=72 n72 72 (n=72 n=75, n75 n=75 , 75 (n=75) 5 P D0. D0 D . (D0) D2 D30 novamente D10 (D10 1 4 3 29,16% 2916 29 16 (29,16% 21,33%, 2133 21,33% 21 33 (21,33%) P>0,05. P005 P>0,05 0 05 (P>0,05) nãogestação 100 28 28% 213 21,3% 398 39 8 39,8% gestante Concluise Conclui monta n=14 n14 14 (n=14 n=7 n7 7 (n=7 (n=75 (D0 D3 D1 (D1 29,16 291 (29,16 21,33 (21,33% P00 P>0,0 (P>0,05 10 21,3 39,8 n=1 n1 (n=1 n= (n= (D 29,1 (29,1 (21,33 P0 P>0, (P>0,0 21, 39, (n 29, (29, (21,3 P>0 (P>0, (29 (21, P> (P>0 (2 (21 (P> ( (P

Abstract Cryoprotectants are required to reduce damage caused to the cells due to low temperatures during the cryopreservation. Antifreeze proteins (AFP) have a well-known role in cell membrane protection, while resveratrol is a potent antioxidant. This study assessed the effect of the association of resveratrol concentrations and AFP I in a ram semen extender. Pooled semen of four rams was allocated into six treatments in a factorial arrangement: (CONT, only the semen extender); only AFP I (ANT: 0.1 µg/mL of AFP I), only resveratrol, one treatment with two levels (10 µM/mL or 50 µM/mL of resveratrol); and two treatments with the interactions, with one AFP I and one of the two levels of resveratrol (0.1 µg/mL of AFP I with 10 µM/mL resveratrol; 0.1 µg/mL of AFP I with 50 µM/mL resveratrol). No interaction between factors was observed on sperm kinetics, plasma membrane integrity, hypo-osmotic test, and mitochondrial activity parameters. There was a high probability (P = 0.06) of reducing sperm cells with functional membrane percentage in the hypo-osmotic test and increasing the percentage of sperm with high mitochondrial activity (P = 0.07) was observed in AFP presence. An interaction of AFP and resveratrol was observed in non-capacitated sperm (P = 0.009), acrosomal reaction (P = 0.034), and sperm binding (P = 0.04). In conclusion, the association of resveratrol and AFP did not improve the quality of frozen-thawed semen and even promoted deleterious effects compared to their single addition in the semen extender. The supplementation of 50 µM/mL of resveratrol improved the outcomes of frozen-thawed ram sperm, being a potential cryoprotectant. cryopreservation (AFP wellknown well known protection antioxidant extender arrangement CONT, CONT (CONT extender) ANT (ANT 01 0 1 0. µgmL µg mL I, , I) (1 µMmL µM 5 resveratrol) interactions (0. resveratrol. . kinetics integrity hypoosmotic hypo osmotic parameters P 0.06 006 06 0.07 007 07 presence noncapacitated non capacitated 0.009, 0009 0.009 009 0.009) 0.034, 0034 0.034 034 0.034) 0.04. 004 0.04 04 0.04) conclusion frozenthawed frozen thawed cryoprotectant ( (0 0.0 00 000 0.00 003 0.03 03