The acetalization or transacetalization reactions produce acetals or ketals used in cosmetics, food, pharmaceuticals, and fuel additives. Besides synthesizing several products, the acetalization or transacetalization reactions can protect carbonyl groups, 1,2 and 1,3-diols in multi-step synthesis. This work studies the transacetalization of glycerol with 2,2-dimethoxypropane (2,2-DMP) to yield 2,2-dimethyl-4-hydroxymethyl-1,3-dioxolane (solketal) by catalyzing with USY, HBeta, and HZSM-5 zeolites under cleaner and mild reaction conditions (reaction temperature: 25 °C; 2,2-DMP/glycerol molar ratio: 1; reaction time: 1 h; and catalyst concentration: 0.05-5 wt.%). For catalyst concentration equal to or greater than 2,5 wt.%, glycerol conversion stabilized near 96-97% for the three zeolites tested. The product selectivities also assume a constant value, close to 97% for solketal. For the lowest concentration of catalyst, the influence of the zeolite properties on the glycerol conversion, such as acidity and the strong/weak acid site ratio, was observed. Concerning the selectivity of the products, the results suggested that it can be based on zeolite topology, active sites at the particle surface, and the relative amount of micro and mesoporous HBeta, the most selective catalyst for acetals production, was reused at least once without loss of activity and selectivity. cosmetics food pharmaceuticals additives products groups 12 2 1, 1,3diols 13diols diols 1,3 3 multistep multi step synthesis 2,2dimethoxypropane 22dimethoxypropane dimethoxypropane 2,2 2,2DMP 22DMP DMP (2,2-DMP 2,2dimethyl4hydroxymethyl1,3dioxolane 22dimethyl4hydroxymethyl13dioxolane dimethylhydroxymethyldioxolane dimethyl 4 hydroxymethyl dioxolane solketal (solketal USY HBeta HZSM5 HZSM 5 HZSM- temperature °C C 2,2DMP/glycerol 22DMPglycerol DMPglycerol DMP/glycerol ratio time h 0.055 0055 0.05 0 05 0.05- wt.%. wt wt.% . wt.%) 2, 9697% 9697 96 97 96-97 tested value strongweak strong weak observed topology surface production 3diols 13 2dimethoxypropane 22 2DMP 2dimethyl4hydroxymethyl1 3dioxolane 055 005 0.0 wt. 969 9 96-9 dimethylhydroxymethyl 2dimethyl4hydroxymethyl 00 0. 96-

Cupuaçu (Theobroma grandiflorum Schum.) is an Amazonian fruit rich in antioxidants potential due to its phenolic compounds. Its seeds produce cupulate, a chocolate-like product, and the residue remains rich in biocompounds. This study aimed to optimize and compare microwave-assisted (MAE) and high pressure/temperature extraction (HPTE) of polyphenolic and flavonoid compounds from cupuaçu seed by-products. A Box-Behnken 33 factorial design assessed the influence of extraction time, solid/liquid ratio, and ethanol concentration. Responses included total polyphenol content, total flavonoid content, and anti-radical power via the ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid) method. In optimum conditions, MAE had the highest total polyphenol content and HPTE for flavonoids. Optimal conditions for both methods were 65% (m/v) ethanol concentration, 45 min extraction time, and 0.03 g mL−1 solid/liquid ratio. MAE and HPTE achieved greater recovery of (−)-epicatechin and (−)-epigallocatechin-3-gallate compared to percolation. Both techniques proved viable for recovering polyphenols from cupuaçu seed by-products, in less time and with less solvent. Thus, cupuaçu by-products can be a sustainable and functional food source, with MAE and HPTE providing high yields and quality extracts rich in polyphenols. Theobroma Schum. Schum cupulate chocolatelike chocolate like product biocompounds microwaveassisted microwave assisted (MAE pressuretemperature pressure temperature (HPTE byproducts. byproducts by products. products BoxBehnken Box Behnken 3 solidliquid solid liquid ratio concentration antiradical anti radical 2,2azinobis3ethylbenzothiazoline6sulphonic 22azinobis3ethylbenzothiazoline6sulphonic azinobisethylbenzothiazolinesulphonic 2,2 azino bis ethylbenzothiazoline 6 sulphonic 2 acid method flavonoids 65 m/v mv m v (m/v 4 003 0 03 0.0 mL1 mL 1 mL− −epicatechin epicatechin − −epigallocatechin3gallate epigallocatechin3gallate epigallocatechingallate epigallocatechin gallate percolation byproducts, products, solvent Thus source 2azinobis3ethylbenzothiazoline6sulphonic azinobis 22 2, 00 0.

To discover new bisamide compounds with insecticidal and fungicidal activities, 19 novel cyproflanilide derivatives containing 1,2,4-oxadiazole group were designed and synthesized according to the principle of biologically active factor splicing. Preliminary biological assay data indicated that the majority of target compounds demonstrated significant insecticidal activity against Lepidopteran pests. Meanwhile, compounds 5p and 5q showed 97.22 and 100% lethality against Tetranychus cinnabarinus at 400 mg L-1, which were better than cyproflanilide (41.11% at 400 mg L-1). In addition, it is exciting that we have achieved our initial goals as some of the target compounds exhibited both insecticidal and fungicidal activities. For instance, compounds 5b and 5c showed 59.26 and 74.07% fungicidal activities against Cucumber downy mildew at 400 mg L-1, as well as 100% insecticidal activity against Plutella xylostella and armyworm at 100 mg L-1. Compound 5h showed 100% insecticidal activity against armyworm at 10 mg L-1, and also displayed 66.67% fungicidal activity against Cucumber downy mildew at 400 mg L-1. The inhibition rate of compounds 5l and 5s against Cucumber downy mildew reached 88.89 and 96.30% at 400 mg L-1. This work showed the effectively fungicidal application of 1,2,4-oxadiazole group in bisamide compounds and provides insights for optimal structural design. 1 1,2,4oxadiazole 124oxadiazole oxadiazole 1,2,4 2 4 splicing pests Meanwhile p q 9722 97 22 97.2 40 L1, L1 L 1, L-1 41.11% 4111 41 11 (41.11 L1. . L-1) addition instance b c 5926 59 26 59.2 7407 74 07 74.07 1. h 6667 66 67 66.67 l s 8889 88 89 88.8 9630 96 30 96.30 design 4oxadiazole 124 1,2, 972 9 97. L- 41.11 411 (41.1 592 5 59. 740 7 0 74.0 666 6 66.6 888 8 88. 963 3 96.3 12 1,2 41.1 (41. 74. 66. 96. 41. (41 (4 (

ABSTRACT: China is the biggest muskmelon producing country across the world. Extreme application of fertilizer during planting of muskmelon poses a serious threat to the environment and food safety, so how to promote farmers to use organic fertilizer has become an important topic. This study creatively introduced green awareness into the extended framework of theory of planned behavior to explore the decisive factors of muskmelon farmers’ behavior intention to apply green organic fertilizer. The structural equation model is used for investigating behavior intention of 543 muskmelon farmers in Leting County, China. The results suggested that both subjective norm and green awareness can affect muskmelon farmers’ behavior intention positively and significantly. When the subjective norm increases by 1 standard deviation, the farmers’ intention increases by 1.028 standard deviation; for every 1 standard deviation increase in green awareness, the farmers’ intention increased by 0.703 standard deviation. ABSTRACT world safety topic 54 County significantly 1028 028 1.02 0703 0 703 0.70 5 102 02 1.0 070 70 0.7 10 1. 07 7 0.

RESUMO: A China é o maior país produtor de melão em todo o mundo. A aplicação extrema de fertilizantes durante o plantio de melão representa uma séria ameaça ao meio ambiente e à segurança alimentar, portanto, como promover os agricultores a usar fertilizante orgânico verde tornou-se um tópico importante. Este estudo introduziu criativamente a consciência verde no quadro estendido da teoria do comportamento, planejado para explorar os fatores decisivos da intenção dos agricultores de melão de aplicar fertilizante orgânico verde. O modelo de equações estruturais é usado para investigar a intenção de comportamento de 543 agricultores de melão no Condado de Leting, China. Os resultados sugerem que tanto a norma subjetiva quanto a consciência verde podem afetar positiva e significativamente o comportamento dos produtores de melão. Quando a norma subjetiva aumenta em 1, o desvio padrão, a intenção do agricultor aumenta em 1,028 desvio padrão. Para cada aumento de 1 desvio padrão na consciência verde, a intenção dos agricultores aumentou em 0,703 desvio padrão. RESUMO mundo alimentar portanto tornouse tornou se importante 54 Leting 1028 028 1,02 0703 0 703 0,70 5 102 02 1,0 070 70 0,7 10 07 7 0,

ABSTRACT: Tomatoes (Solanum lycopersicum L.) are nutrient-rich fruits with a high glutamic acid content, making them a suitable source for producing γ-aminobutyric acid (GABA) through the action of lactic acid bacteria (LAB). LAB plays a pivotal role in lactic fermentation, enhancing the taste and nutritional value of food products, often contributing to their health benefits. This study isolated GABA-producing LAB from tomatoes and assessed their potential for producing GABA-enriched fermented tomato juice. The results indicated the isolation of 15 LAB strains from the samples of five different tomatoes. All of these strains demonstrated the capability to produce GABA, with levels ranging from 1.732 to 3.113 mg/mL, as determined using thin-layer chromatography (TLC). Through sequencing, the promising strain TO42, identified as Weissella cibaria, was selected for tomato juice fermentation. Furthermore, GABA-enriched tomato juice was successfully fermented at 15 °Brix for 72 h, resulting in the highest recorded GABA and lactic acid content of 9.185 ± 0.398 mg/mL and 13.05 ± 1.56 g/L, respectively. ABSTRACT Solanum L. L nutrientrich nutrient rich γaminobutyric γ aminobutyric (GABA LAB. . (LAB) fermentation products benefits GABAproducing GABAenriched enriched 1 1732 732 1.73 3113 3 113 3.11 mgmL mg mL thinlayer thin layer TLC. TLC (TLC) sequencing TO42 TO cibaria Furthermore Brix 7 h 9185 9 185 9.18 0398 0 398 0.39 1305 13 05 13.0 156 56 1.5 gL g g/L respectively (LAB 173 73 1.7 311 11 3.1 (TLC TO4 918 18 9.1 039 39 0.3 130 13. 5 1. 17 31 3. 91 9. 03 0.

RESUMO: O tomate (Solanum lycopersicum L.) é uma fruta rica em nutrientes e com alto teor de ácido glutâmico, tornando-o uma fonte adequada para a produção de ácido γ-aminobutírico (GABA) através da ação de bactérias ácido lácticas (BAL). As BAL desempenham um papel fundamental na fermentação láctica, melhorando o sabor e o valor nutricional dos produtos alimentares, contribuindo frequentemente para os seus benefícios para a saúde. Este estudo teve como objetivo isolar BAL produtoras de GABA de tomates e avaliar seu potencial para a produção de suco de tomate fermentado enriquecido com GABA. Os resultados indicam o isolamento de 15 cepas de BAL a partir de amostras de cinco tomates diferentes. Todas essas cepas demonstraram capacidade de produzir GABA, com níveis variando de 1,732 a 3,113 mg/mL, conforme determinado por cromatografia em camada delgada (CCD). Através de sequenciamento, a cepa promissora TO42, identificada como Weissella cibaria, foi selecionada para fermentação de suco de tomate. Além disso, o suco de tomate enriquecido com GABA foi fermentado com sucesso a 15 °Brix por 72 h, resultando no maior teor registrado de GABA e ácido láctico de 9.185 ± 0.398 mg/mL e 13.05 ± 1.56 g/L, respectivamente. RESUMO Solanum L. L glutâmico tornandoo tornando γaminobutírico γ aminobutírico (GABA BAL. . (BAL) láctica alimentares saúde 1 diferentes 1732 732 1,73 3113 3 113 3,11 mgmL mg mL CCD. CCD (CCD) sequenciamento TO42 TO cibaria disso Brix 7 h 9185 9 185 9.18 0398 0 398 0.39 1305 13 05 13.0 156 56 1.5 gL g g/L respectivamente (BAL 173 73 1,7 311 11 3,1 (CCD TO4 918 18 9.1 039 39 0.3 130 13. 5 1. 17 1, 31 3, 91 9. 03 0.

Abstract Purpose: To identify the main links between the Localized Agri-Food Systems (SYAL = Systemes Agroalimentaires Localisés) approach and agroecology and determine the elements of agroecology that could strengthen SYAL to promote territorial patrimonialization. Methodological design: Through the qualitative approach and based on the systematic review of documentary and multimedia sources in the period 2010-2023, a content analysis was carried out to propose a critical path for territorial patrimonialization. Results: Both approaches seek the reconnection of food with the territory and recognize a quality that results from processes and products according to bioregions; distinguishing the territory as an element of identity; valuating traditional know-how and promoting local, collective action. Research limitations: There is a gap of studies from the SYAL approach that delves into the application of agroecological practices as a strategy to strengthen the quality linked to the territory, address the environmental dimension, and promote territorial patrimonialization. Findings: The agroecological principles emphasize the quality linked to the territory, and the SYAL approach the bioregional patrimonialization.

Resumen Objetivo: Identificar los principales vínculos entre el enfoque de Sistemas Agroalimentarios Localizados (SIAL) y el agroecológico y determinar los elementos de la agroecología que podrían fortalecer al SIAL para promover la patrimonialización territorial. Diseño metodológico: A través del enfoque cualitativo y partiendo de la revisión sistemática de fuentes documentales y multimedia en el periodo 2010-2023, se llevó a cabo un análisis de contenido a fin de proponer una ruta crítica para la patrimonialización territorial. Resultados: Ambas aproximaciones buscan la reconexión de los alimentos con el territorio y reconocen una calidad que resulta de procesos y productos anclados a biorregiones; distinguen al territorio como elemento identitario; valorizan los saber-hacer tradicionales e impulsan la acción colectiva local. Limitaciones de la investigación: Existe un vacío de estudios desde el enfoque SIAL que profundicen en la aplicación de prácticas agroecológicas como estrategia para fortalecer la calidad ligada al territorio, atender la dimensión ambiental y promover la patrimonialización territorial. Hallazgos: Los principios agroecológicos enfatizan la calidad ligada al territorio y el enfoque SIAL la patrimonialización biorregional.

Abstract Introduction: Various anthropic pressures affect the aquatic ecosystems of the foothills of Colombia. The response to environmental stressors is still unknown in bioindicator organisms such as Leptohyphidae. Objective: To determine the diversity of Leptohyphidae nymphs of the Quenane-Quenanito river, in two contrasting hydrological periods and its relationship with some physicochemical variables. Methods: In December (2014) and February (2015), organisms were collected with a Surber net at six stations along the current. Alpha and beta diversity was analyzed and redundancy analysis and generalized linear model were applied to establish the relationship between taxa and environmental variables. Results: Were identified 369 organisms belonging to four genera (Amanahyphes, Traverhyphes, Tricorythopsis, and Tricorythodes), two species, and eight morphospecies. Amanahyphes saguassu is reported for the first time for the Meta department. High diversity of Leptohyphidae nymphs was recorded in the transition to drought season and greater abundance in drought. Beta diversity indicated that the configuration of the assemblage changes spatially and temporally. Conclusions: Leptohyphidae organisms prefer fast habitats, particularly in the dry period where they find food (leaf litter, detritus) and shelter to establish themselves successfully; anthropic activities such as urbanization notably affect diversity. The high diversity recorded in this small river in the foothills of the plains reflects the need to increase this type of works and collection efforts of study material in the region.

Resumen Introducción: Varias presiones antrópicas sufren los ecosistemas acuáticos del piedemonte llanero en Colombia. La respuesta a estresores ambientales aún se desconoce en organismos bioindicadores como Leptohyphidae. Objetivo: Determinar la diversidad de ninfas de Leptohyphidae del río Quenane-Quenanito, en dos periodos hidrológicos contrastantes y su relación con algunas variables fisicoquímicas. Métodos: En diciembre (2014) y febrero (2015) se recolectaron organismos con red Surber en seis estaciones a lo largo del río. Se analizó la diversidad alfa y beta y se aplicó análisis de redundancia y modelos lineales generalizados con el fin de establecer la relación entre los taxones y las variables ambientales. Resultados: Se identificaron 369 organismos pertenecientes a cuatro géneros (Amanahyphes, Traverhyphes, Tricorythopsis y Tricorythodes), dos especies y ocho morfoespecies. Se reporta por primera vez para el departamento del Meta Amanahyphes saguassu. Se registró la mayor diversidad de ninfas en la transición a la sequía y la mayor abundancia en sequía. La diversidad beta señaló que la configuración del ensamblaje cambia a nivel espacial y temporal. Conclusiones: Los organismos de Leptohyphidae prefieren hábitats de corrientes, particularmente en el periodo de sequía, donde hallan alimento (hojarasca, detritos) y refugio para establecerse exitosamente; actividades antrópicas como la urbanización afectan notablemente la diversidad. La alta diversidad registrada en este pequeño río de piedemonte llanero refleja la necesidad de incrementar este tipo de trabajos y esfuerzos de recolección de material de estudio en la región.

Abstract Introduction: Aquatic birds (AB) are usually associated with wetlands, which provide refuge, food, and/or nesting sites for resident and migratory species. Despite their ecological importance, there is little knowledge on AB in some tropical environments, such as those found on the Colima coast. Objective: To investigate the spatial and temporal composition of the AB community in Juluapan Lagoon, Colima, Central Mexican Pacific. Methods: Monthly counts were conducted between June 2017 and May 2018 during low-tide conditions to record habitat use by AB. Species richness and bird counts were obtained to compare sampling areas; mean richness and number of individuals were compared between seasons. Results: We detected 53 species and 5 750 individuals. The highest species richness and relative abundance values were obtained in winter at the lagoon area farthest from the connection with the marine system, where anthropogenic activity is lower. Diversity was greater in zones 2 and 3 in spring, summer, and fall. Muddy flats were the most used environment, and the most frequent activity was resting. Nesting activity was only recorded in the middle of the lagoon at the mangrove during spring. “Shorebirds” and “waders” were the most dominant groups in the bird community of the Juluapan lagoon. Conclusions: This coastal wetland is a site of great biological importance for aquatic birds; thus, conservation measures should be implemented, and there should be a continuous study of the effects of anthropogenic pressure.

Resumen Introducción: Las aves acuáticas (AA) son usualmente relacionadas a los humedales debido a que éstos funcionan como sitios de refugio, alimentación y anidación de diferentes especies residentes y migratorias. Sin embargo, el conocimiento sobre las aves acuáticas en algunos humedales es nulo. Objetivo: Investigar la composición espacio-temporal de la comunidad de AA en la laguna Juluapan, Colima, en el Pacífico Central Mexicano. Métodos: Entre junio de 2017 y mayo de 2018 se llevaron a cabo conteos mensuales en condiciones de marea baja para registrar el uso de hábitat de las AA. Se obtuvieron valores de riqueza de especies y número de individuos para realizar comparaciones entre zonas de muestreo, así como el promedio del número de especies y número de individuos para comparaciones entre temporadas. Resultados: Se registraron un total de 53 especies y 5 750 individuos. Los valores de riqueza de especies y densidad de individuos fueron más altos durante invierno, en la zona más alejada al ambiente marino, donde la actividad antropogénica es menor. La diversidad tuvo valores más altos en la zona 2 y 3, durante primavera, verano y otoño. El ambiente más explotado por las aves fueron las planicies lodosas; y el descanso fue la actividad más frecuente. Asimismo, la actividad de anidación sólo se registró en el manglar de la zona media durante primavera. Las “aves playeras” y “aves zancudas” fueron los grupos más predominantes en la comunidad de aves de la laguna Juluapan. Conclusiones: Este humedal costero es un sitio de gran importancia biológica para aves acuáticas, por lo que resulta necesario la implementación de medidas de conservación, así como el estudio de los efectos por la presión antropogénica.

Resumen Objetivo: este estudio estima un sistema de demanda de bienes alimenticios en México, incorporando dimensiones territoriales y de ingreso. Se evalúa el impacto diferenciado de choques en los precios en el bienestar económico y la tasa de pobreza, considerando variaciones entre hogares y entre cinco grandes regiones. Metodología: utilizando el modelo de valores unitarios de Deaton (1988) y la Encuesta Nacional de Ingresos y Gastos de los Hogares (ENIGH) de 2018, calculamos elasticidades-precios y elasticidades-ingreso y el impacto de las variaciones de precios en el ingreso y la pobreza. Resultados: revelan importantes diferencias regionales y se subraya la necesidad de considerar diferencias socioeconómicas y regionales y de adoptar un enfoque territorial en este tipo de análisis y para formular políticas efectivas. Limitaciones: existen limitaciones a este estudio que pueden afectar la comprensión completa de la dinámica de la pobreza. Hemos enfocado nuestra atención únicamente en los bienes alimenticios, excluyendo un grupo específico de productos, en este caso, los productos pesqueros. También, se considera que los hogares son únicamente consumidores de bienes, omitiendo así la dimensión productiva de autoconsumo. Originalidad: radica en la vinculación directa entre las estimaciones de pobreza y la desagregación territorial, integra patrones de consumo y elasticidades específicas a cada región. Conclusiones: se resalta la importancia de considerar las diferencias socioeconómicas y regionales al analizar la demanda de bienes alimenticios y su impacto en la pobreza. A pesar de las limitaciones, nuestros hallazgos subrayan la necesidad de un enfoque territorial en este tipo de análisis para formular políticas adaptadas y efectivas de reducción de la pobreza en diferentes regiones del país.

Abstract Objective: this study estimates a system of demand for food products in Mexico, incorporating territorial and income dimensions. The differentiated impact of shocks on prices on economic well-being and the poverty rate is evaluated, considering variations among households and across five major regions. Methodology: using Deaton’s (1988) unit values model and the 2018 National Income and Expenditure Survey (ENIGH), we calculate price and income elasticities, examining the impact of price variations on income and poverty. Results: our results reveal significant regional differences, emphasizing the need to consider socioeconomic and regional variations and to adopt a territorial approach in such analyses for effective policy formulation. Limitations: there are limitations to this study that may affect a complete understanding of poverty dynamics. We have focused our attention solely on food products, excluding a specific group of products, in this case, fishery products. We also assume that households are only consumers of goods, thus omitting the productive and self-consumption dimension. Originality: lies in the direct link between poverty estimates and territorial disaggregation, integrating consumption patterns and specific elasticities for each region. Conclusions: our study highlights the importance of considering socioeconomic and regional differences when analyzing the demand for food products and its impact on poverty. Despite the limitations, our findings underscore the need for a territorial approach in this type of analysis to formulate tailored and effective poverty reduction policies in different regions of the country.

Summary: The state of Chiapas presents degradation processes of dif ferent kinds, as a consequence of the current established agricultural model. It is estimated that, in the near future, the surface of the state of Chiapas will present more than 55% degradation, generating changes in the physical, chemical and biological properties of the soil, af fecting the quality of life of human beings, mainly due to scarcity of food. The objective of this research was to evaluate the quality of the soils in three municipalities of the central depression of Chiapas, Mexico. The analysis were interpreted taking NOM-021-SEMARNAT-2000 as a reference and were classified according to the world reference base (WRB), version 2014. For the evaluation of soil quality, the sustainable use of land subindex (SUSS). 144 soil samples corresponding to 21 profiles were analyzed. Of the 21 profiles studied, 17 present poor soil qualities, two a sensitive quality, one acceptable quality and one with good quality. Only two soil groups presented good fertility and are classified as Vertisols and Phaeozems, which present good quality and capacity to maintain sustainable agricultural production in this region.

Resumen: El estado de Chiapas presenta procesos de degradación de diferente índole, como consecuencia del actual modelo agrícola establecido. Se estima que, en el futuro cercano, la superficie del estado de Chiapas presente más de 55% de degradación, generando modificaciones en las propiedades físicas, químicas y biológicas del suelo; afectando la calidad de vida de los seres humanos, principalmente por escasez de alimentos. El objetivo de esta investigación fue evaluar la calidad de los suelos en tres municipios de la depresión central de Chiapas, México. Los análisis se interpretaron tomando como referencia la NOM-021-SEMARNAT-2000 y se clasificaron de acuerdo con la world reference base (WRB), versión 2014, para la evaluación de calidad del suelo se utilizó el subíndice de uso sustentable del suelo (SUSS). Se analizaron 144 muestras de suelo correspondientes a 21 perfiles. De los 21 perfiles estudiados, 17 presentan calidad de suelo pobre, dos calidades sensibles, uno calidad aceptable y uno con calidad buena. Solo dos Grupos de suelo presentaron buena fertilidad y son clasificados como Vertisols y Phaeozems, los cuales presentan buena calidad y capacidad para mantener producción agrícola sostenible en esta región.

Summary: Excessive use of chlorpyrifos affects the quality of agricultural soils causing negative effects on the safety of harvested food. The behavior of chlorpyrifos residues depends on soil properties, especially organic matter. Our aim in this research was to determine the effect of the application of vermicompost on the residual and dissipation of chlorpyrifos in an entisol soil of semi-arid climate with variations in its edaphic fertility. Four treatments were studied. T1: 7.5% vermicompost (V) and 92.5% soil (S); T2: 5% V and 95% S; T3: 2.5% V and 97.5% S; T4: 0% V and 100% S. The treatments were subjected to a seven-day incubation period. Subsequently, a dose of chlorpyrifos of 1.5 mg kg-1 as active ingredient was applied once for each treatment. A completely randomized design was used with three replicates for each treatment, forming 12 experimental units. The analytical determination of chlorpyrifos was carried out by high performance liquid chromatography (HPLC). The evaluations were carried out 1.7, 14, 28, 42, 70 and 98 days after the application of chlorpyrifos. The results showed that the incorporation of vermicompost to the soil at increasing levels promoted a decrease in the residual concentration of chlorpyrifos and facilitated its gradual dissipation. The chlorpyrifos dissipation curves correspond to a first order logarithmic equation where the effects of the treatments caused greater dissipation of chlorpyrifos as the evaluation period elapsed. At 98 days of evaluation, T1 registered lower residuality (0.786 m kg-1), higher daily dissipation rate (0.008) and lower half-life of chlorpyrifos (86.64 days). At the end of the research period, edaphic levels of P, K, EC and CEC increased while OM, N and pH decreased after vermicompost application.

Resumen: El uso excesivo de clorpirifos afecta la calidad de los suelos agrícolas provocando efectos negativos en la inocuidad de los alimentos cosechados. El comportamiento de los residuos de clorpirifos depende de las propiedades edáficas especialmente la materia orgánica. El objetivo de la investigación fue determinar el efecto de la aplicación de vermicompost en la residualidad y disipación de clorpirifos en un suelo entisol de clima semiárido con variaciones en su fertilidad edáfica. Se estudiaron cuatro tratamientos. T1: 7.5% vermicompost (V) y 92.5% suelo (S); T2: 5% V y 95% S; T3: 2.5% V y 97.5% S; T4: 0% V y 100% S. Los tratamientos fueron sometidos a un periodo de incubación de siete días. Posteriormente, se aplicó una dosis de clorpirifos de 1.5 mg kg-1 como ingrediente activo en una sola oportunidad por cada tratamiento. Se utilizó un diseño completamente al azar con tres repeticiones por cada tratamiento conformando 12 unidades experimentales. La determinación analítica de clorpirifos se efectuó por cromatografía líquida de alta resolución (HPLC). Las evaluaciones se realizaron a 1,7, 14, 28, 42, 70 y 98 días de la aplicación de clorpirifos. Los resultados demostraron que la incorporación de vermicompost al suelo en niveles crecientes promovió la disminución de la concentración residual de clorpirifos y facilitó su disipación de manera gradual. Las curvas de disipación de clorpirifos corresponden a una ecuación logarítmica de primer orden donde los efectos de los tratamientos ocasionaron mayor disipación de clorpirifos conforme transcurrió el periodo de evaluación. A 98 días de evaluación el T1 registró menor residualidad (0.786 m kg-1), mayor tasa de disipación diaria (0.008) y menor vida media de clorpirifos (86.64 días). Al final del periodo de investigación, los niveles edáficos de P, K, CE y CIC se incrementaron mientras que la MO, N y pH descendieron luego de la aplicación de vermicompost.

Resumen La bacteria Escherichia coli causa la colibacilosis en animales de granja que actúan como reservorios de cepas patógenas. La resistencia antimicrobiana de E. coli productor de betalactamasas de espectro extendido [BLEE] es un grave problema de salud pública y se puede atribuir a factores relacionados con el consumo de alimentos y el contacto con animales domésticos. El objetivo de este estudio fue determinar la presencia y patrones de resistencia antimicrobiana de E. coli BLEE aislado en muestras fecales provenientes de bovinos productores de leche de la provincia de Pichincha. Se analizaron un total 182 muestras de heces de bovinos: 112 muestras de bovinos faenados en el Camal Metropolitano de la provincia de Pichincha y 70 muestras de la colección de la Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencias a los Antimicrobianos [UNIETAR], se realizó el aislamiento de E. coli BLEE, la identificación bioquímica y pruebas de resistencia a los principales antibióticos utilizados. Se logró identificar 93 muestras positivas a E. coli BLEE (51 %), el análisis fenotípico reveló que los antibióticos amoxicilina más ácido clavulánico, cefepime, ceftazidima, ciprofloxacina, amikacina y tetraciclina presentaron porcentajes de resistencia mayores al 80 %. Además, se observó una baja resistencia a la nitrofurantoína, cefoxitin y ertapenem, mientras que ningún aislado fue resistente a la tigeciclina. El 100 % de los aislados presentaron fenotipos de multirresistencia y el patrón más frecuente estuvo compuesto por 7 familias de antibióticos. En conclusión, estos resultados sugieren que E. coli originaria de bovinos lecheros podría ser un reservorio de genes BLEE.

Abstract Escherichia coli causes colibacillosis in farm animals that act as reservoirs for pathogenic strains. Antimicrobial resistance of E. coli producing Extended Spectrum Beta-lactamases [ESBL] is a serious public health problem that can be attributed to factors related to food consumption and contact with domestic animals. This study aimed to determine the presence and patterns of antimicrobial resistance of ESBL E. coli isolated from fecal samples from dairy cattle in the Pichincha province. A total of 182 bovine feces samples were analyzed, 112 samples from cattle slaughtered at the official slaughterhouse in Quito-Pichincha province, and 70 samples from the collection of the Foodborne Diseases and Antimicrobial Resistance Research Unit [UNIETAR]. The isolation of ESBL E. coli, biochemical identification, and resistance tests using the main antibiotics were carried out at UNIETAR. It was possible to identify 93 positive samples for ESBL E. coli (51%), phenotypic analysis revealed that antibiotics such as amoxicillin plus clavulanic acid, cefepime, ceftazidime, ciprofloxacin, amikacin, tetracycline, presented resistance higher than 80%. Furthermore, low resistance to nitrofurantoin, cefoxitin, and ertapenem was observed, while no isolate was resistant to tigecycline. One hundred percent of the isolates presented multi-resistance phenotypes, with the most frequent pattern being composed of 7 families of antibiotics. In conclusion, these results suggest that E. coli from dairy cattle origin could be an important reservoir of ESBL genes.

Abstract Purpose: To identify the main links between the Localized Agri-Food Systems (SYAL = Systemes Agroalimentaires Localisés) approach and agroecology and determine the elements of agroecology that could strengthen SYAL to promote territorial patrimonialization. Methodological design: Through the qualitative approach and based on the systematic review of documentary and multimedia sources in the period 2010-2023, a content analysis was carried out to propose a critical path for territorial patrimonialization. Results: Both approaches seek the reconnection of food with the territory and recognize a quality that results from processes and products according to bioregions; distinguishing the territory as an element of identity; valuating traditional know-how and promoting local, collective action. Research limitations: There is a gap of studies from the SYAL approach that delves into the application of agroecological practices as a strategy to strengthen the quality linked to the territory, address the environmental dimension, and promote territorial patrimonialization. Findings: The agroecological principles emphasize the quality linked to the territory, and the SYAL approach the bioregional patrimonialization.

Resumen Objetivo: Identificar los principales vínculos entre el enfoque de Sistemas Agroalimentarios Localizados (SIAL) y el agroecológico y determinar los elementos de la agroecología que podrían fortalecer al SIAL para promover la patrimonialización territorial. Diseño metodológico: A través del enfoque cualitativo y partiendo de la revisión sistemática de fuentes documentales y multimedia en el periodo 2010-2023, se llevó a cabo un análisis de contenido a fin de proponer una ruta crítica para la patrimonialización territorial. Resultados: Ambas aproximaciones buscan la reconexión de los alimentos con el territorio y reconocen una calidad que resulta de procesos y productos anclados a biorregiones; distinguen al territorio como elemento identitario; valorizan los saber-hacer tradicionales e impulsan la acción colectiva local. Limitaciones de la investigación: Existe un vacío de estudios desde el enfoque SIAL que profundicen en la aplicación de prácticas agroecológicas como estrategia para fortalecer la calidad ligada al territorio, atender la dimensión ambiental y promover la patrimonialización territorial. Hallazgos: Los principios agroecológicos enfatizan la calidad ligada al territorio y el enfoque SIAL la patrimonialización biorregional.

Abstract Purpose: To analyze the contribution of the program "Sembrando Vida" in alleviating the poverty of male and female farmers using an Actor-Oriented Approach (AOA), to comprehend the program's impact on poverty reduction in Acayucan, Veracruz, Mexico. This analysis takes into consideration aspects such as employment, food self-sufficiency, the reconstruction of social fabric, forest recovery, the potential of territories, and overall well-being. Methodological design: This is an ethnographic study, using in-depth interviews and participant observation of ten farmers in the program over a period of seven months. The analysis of the information was carried out using NVivo software. Results: The program is partially fulfilling the objective to decrease poverty, because farmers still confront productive limitations, social vulnerability, higher investments, and technical-productive difficulties. Research limitations: The scope of the research only corresponds to the actors who participated in the research; therefore, the results cannot be generalized. Comparisons with more communities and with farmers not participating in the program would give a broader picture. Findings: This study contributes to the understanding of the impact of public interventions on development processes. The findings contribute to improving the implementation of the program and to developing future projects that consolidate the medium and long-term goals of the program.

Resumen Objetivo: analizar la contribución del programa Sembrando Vida en el alivio de la pobreza de campesinas y campesinos desde un Enfoque Orientado al Actor (EOA), para comprender el impacto del programa en la disminución de la pobreza en Acayucan, Veracruz, México; considerando aspectos como: el empleo, la autosuficiencia alimentaria, la reconstrucción del tejido social, la recuperación forestal, el potencial de territorios y el bienestar. Diseño metodológico: esta investigación cualitativa es de carácter etnográfico, realizando entrevistas a profundidad y observación participante a diez agricultores del programa durante un periodo de siete meses. El análisis de la información se realizó a través del software NVivo. Resultados: el programa está cumpliendo parcialmente con su objetivo de combate a la pobreza, puesto que los campesinos aún enfrentan limitaciones productivas, vulnerabilidad social, mayores inversiones y dificultad técnico-productiva. Limitaciones de la investigación: los alcances de la investigación solo corresponden a los actores que participaron en ella, por lo tanto, los resultados no pueden generalizarse. Comparar con más comunidades y con campesinos no participantes del programa daría una visión más amplia. Hallazgos: se aporta una mayor comprensión del impacto de las intervenciones públicas en los procesos de desarrollo. Lo encontrado suma para la mejora del programa en su aplicación y en el desarrollo de futuros proyectos que consoliden las metas a mediano y largo plazo del programa.

RESUMEN Este trabajo tiene por objetivo presentar un método de evaluación y análisis de la cadena de suministro como estructura organizativa cuya salida es un producto de calidad y alta demanda en el sector agropecuario. Se presentan definiciones, así como los elementos que definen a la cadena de suministro como estructura organizativa. Se brinda un método de evaluación y análisis, estableciendo sus dimensiones de trabajo y las etapas para su desarrollo. Su aplicación práctica permite realizar una síntesis de los factores que entorpecen y/o facilitan la efectividad de la cadena en el contexto de la economía cubana. Se expone el resultado del proyecto de investigación «Mejorar la cadena de suministro de productos cárnicos del sector agroalimentario para la exportación en fronteras. Código: PN131SS014-011», perteneciente a un Programa Nacional de Ciencia y Técnica.

ABSTRAC This work aims to present a method of evaluation and analysis of the supply chain as an organizational structure whose output is a quality product and high demand in the agricultural sector. Definitions are presented, as well as the elements that define the supply chain as an organizational structure. An evaluation and analysis method is provided, establishing its dimensions of work and the stages for its development. Its practical application allows a synthesis of the factors that hinder and/or facilitate the effectiveness of the chain in the context of the Cuban economy. The result of the research project «Improving the supply chain of meat products in the agri-food sector for export at borders Code PN131SS014-011», belonging to add files National Program of Science and Technique