Abstract Introduction. In prepubertal heifers, Kisspeptin-10 has stimulated the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), and in postpartum lactating cows, it has stimulated LH secretion without evidence of inducing ovulation. Objective. To induce ovulation using Kisspeptin-10 or gonadotropin-releasing hormone (GnRH) in beef-producing cows under subtropical conditions in Mexico. Materials and methods. The study was conducted in the northeastern region of the state of Puebla uring the years 2020 to 2021, with 252 Bos taurus x Bos indicus cows. The estrous synchronization protocol involved the insertion of an intravaginal device impregnated with progesterone (1.9 g) and intramuscular injection of 2 mg of estradiol benzoate (day 0). On day seven, the intravaginal device was removed, and cows were immediately intramuscularly injected with 0.150 mg of D-cloprostenol. On the following day (day eight), Kisspeptin-10 was as effective as GnRH in inducing ovulation. 56 hours after the removal of the intravaginal device (day nine), cows were intramuscularly injected with GnRH or Kisspeptin-10. Cows were assigned to four treatments: T1, 100 µg of GnRH (positive control), T2, T3, and T4 with 500 µg, 1000 µg, and 1500 µg of Kisspeptin-10, respectively. Ovulation was detected via ultrasonography four days post-treatment. The response variable evaluated was the ovulation rate. Data were analyzed using generalized linear models with a logit link function. Results. Ovulation rates obtained with T1 and T4 were statistically equal (99.9%; p>0.05) and higher (p<0.05) than those achieved with T2 (98.5%) and T3 (88.4%), with the latter two being statistically equal (p>0.05). Conclusion. Kisspeptin-10 1500 µg was as effective as GnRH in inducing ovulation.

Resumen Introducción. En becerras prepúberes, la Kisspeptina-10 ha estimulado la secreción de las hormonas luteinizante (LH) y folículo estimulante (FSH), y en vacas posparto amamantando ha estimulado la secreción de LH sin evidencia de inducir la ovulación. Objetivo. Inducir la ovulación con Kisspeptina-10 u hormona liberadora de gonadotropinas (GnRH), en vacas productoras de carne en condiciones subtropicales de México. Materiales y métodos. El estudio se realizó en la región nororiente del estado de Puebla durante los años 2020 al 2021, con 252 vacas cruzadas Bos taurus x Bos indicus. El protocolo de sincronización estral consistió en la aplicación de un dispositivo intravaginal impregnado con progesterona (1,9 g) y la inyección intramuscular de 2 mg de benzoato de estradiol (día 0). El día siete se removió el dispositivo intravaginal y de inmediato las vacas fueron inyectadas vía intramuscular con 0,150 mg de D-cloprostenol. Al día siguiente (día ocho), las vacas fueron inyectadas vía intramuscular con 1 mg de benzoato de estradiol. Entre 52 y 56 horas posteriores al retiro del dispositivo intravaginal (día nueve), las vacas se inyectaron vía intramuscular con GnRH o Kisspeptina-10. Las vacas se asignaron a cuatro tratamientos: T1, 100 µg de GnRH (control positivo), T2, T3 y T4 500 µg, 1000 µg y 1500 µg de Kisspeptina-10, respectivamente. La ovulación se detectó mediante ultrasonografía a los cuatro días post-tratamiento. La variable de respuesta evaluada fue la tasa de ovulación. Los datos se analizaron mediante modelos lineales generalizados y se aplicó una función liga logit. Resultados. Las tasas de ovulación obtenidas con T1 y T4 fueron iguales estadísticamente (99,9 %; p>0,05) y mayores (p<0,05) que las logradas con T2 (98,5 %) y T3 (88,4 %), estas dos últimas fueron iguales estadísticamente (p>0,05). Conclusión. La Kisspeptina-10 1500 µg fue tan efectiva como la GnRH para inducir la ovulación.

Abstract Chronic ulcers significantly affect the quality of life of patients and impose a high cost on the healthcare system. The therapeutic management should be comprehensive, taking into consideration the etiological diagnosis of the wound and the characteristics of the wound bed when deciding on a therapeutic proposal appropriate to the healing phase, correcting factors that delay healing. During the epithelialization phase, repair techniques with grafts are recommended to shorten re-epithelialization time, improve the quality of scar tissue, and achieve adequate pain management. Currently, due to the reported benefits of skin appendages, the technique of follicular unit auto-grafting obtained with a scalp punch is among the chosen strategies for wound repair. This is a minimally invasive, outpatient practice, whose technique has advantages over the donor site, patients recovery and well-being. system comprehensive phase reepithelialization re time tissue Currently appendages autografting auto grafting invasive practice site wellbeing. wellbeing well being. being well-being

Resumo O cisto dentígero pode ser classificado como cisto de desenvolvimento ou inflamatório, originando-se durante o processo de separação do folículo que fica aprisionado ao redor da coroa de um dente incluso. O objetivo do presente artigo é relatar um caso clínico de enucleação de cisto dentígero em corpo mandibular à esquerda associado ao primeiro molar inferior (36) incluso com envolvimento do nervo alveolar inferior através da técnica cirúrgica de janela óssea por meio da piezocirurgia. Paciente de 19 anos, sexo masculino, sem comorbidades, com lesão hipodensa, circunscrita, de aproximadamente 15,5 x 27,7 x 14,8 mm identificada em tomografia de feixe cónico. A técnica de janela óssea executada apresenta como vantagem o reposicionamento do segmento ósseo deslocado no transoperatório, favorecendo o processo de reparo, além do acesso direto à área contendo a lesão cística e o elemento incluso.

Abstract A dentigerous cyst can be classified as a developmental or inflammatory cyst. It originates during the process of separation from the follicle that becomes trapped around the crown of an impacted tooth. The purpose of this article is to report a clinical case of enucleation of a dentigerous cyst in the left mandibular body associated with the impacted lower first molar (tooth 36), with involvement of the inferior alveolar nerve, through the bone window surgical technique using piezosurgery. A 19-year-old male patient with no comorbidities presented with a well-defined hypodense lesion of approximately 15.51mm X 27.69mm X 14.79mm in diameter, as identified by cone-beam tomography. The bone window technique performed offers the advantage of repositioning the bone segment displaced during surgery, promoting the repair process, and providing direct access to the area containing the cystic lesion and the impacted tooth.

Resumen El desempeño reproductivo es crucial para la rentabilidad de la producción doble propósito en la Amazonía Ecuatoriana. Evaluar tres protocolos para Inseminación Artificial a tiempo fijo (IATF) en vacas en lactancia de doble propósito. Vacas Pardo Suizo en lactancia (n=301) recibieron 2 mg de benzoato de estradiol (EB) y un dispositivo intravaginal con 0,5 g de progesterona (P4) el Día 0 y fueron asignadas aleatoriamente en tres grupos. Las vacas del grupo EB recibieron 500 μg de cloprostenol (PGF2α) y se les retiró el dispositivo el Día 7, y recibieron 1 mg de EB el Día 8. Las vacas del grupo ECP fueron tratadas como las del grupo EB, excepto que recibieron 0,5 mg de cipionato de estradiol (ECP) en el día del retiro del dispositivo (Día 7) en lugar de EB el Día 8. En las vacas del grupo J-Synch, el retiro del dispositivo y la administración de PGF2α se realizaron el Día 6. Todas las vacas fueron IATF el Día 9 y las del grupo J-Synch recibieron 100 μg de gonadorelina en ese momento. Aunque el diámetro del folículo dominante y del CL fueron mayores (P<0,05) en las vacas del grupo J-Synch, las tasas de preñez a la IATF no difirieron entre los grupos (EB: 51,0%, ECP: 53,0% y J-Synch: 59,4%). Los tres protocolos probados se pueden aplicar con éxito en vacas de doble propósito amamantando.

Abstract Reproductive performance is crucial for profitability of dual-purpose cow-calf production in the Ecuadorian Amazon. To evaluate three different fixed-time artificial insemination (FTAI) protocols in suckled, dual-purpose cows in the Ecuadorian Amazon. Lactating, Brown Swiss cows (n=301) received 2 mg estradiol benzoate (EB) and an intravaginal device containing 0.5 g of progesterone (P4) on Day 0. They were allocated randomly into the following three treatment groups: Cows in the EB group received 500 μg cloprostenol (PGF2α), and P4 devices were removed on Day 7, and 1 mg EB was administered on Day 8. Cows in the ECP group were treated as those in the EB group, except that they received 0.5 mg estradiol cypionate (ECP) at the time of P4 device removal on Day 7 instead of EB on Day 8. Cows in the J-Synch group had the P4 device removed and PGF2α administered on Day 6. All cows were FTAI on Day 9; cows in the J-Synch group also received 100 μg gonadorelin at that time. Although the diameter of the dominant follicle and the resulting CL were greater (P<0.05) in cows in the J-Synch group, pregnancies per AI did not differ (P>0.2) among groups (EB: 51.0%, ECP: 53.0% and J-Synch: 59.4%). The three protocols tested were applied successfully in suckled, dual-purpose cows with no differences in pregnancies per AI.

Abstract Environmental pollution has the potential to have a significant impact on animal’s health especially on birds due to daily exposure and habitat. This experimental study was carried out for a 60 days period in which, a total of 24 pigeon birds with suitable weight (80-100 g) were kept in Animal house with suitable environmental conditions viz, controlled temperature, humidity & light source to minimize any other stress. Out of twenty-four, eighteen birds were divided into three treatment groups (6 birds in each group). Whole experiment was run in triplicate manner in breeding season. One served as Control (Group 1) and remaining three were experimental groups including Road traffic noise (Group 2), Military noise (Group 3) & Human activities noise (Group 4). Noise was applied as recorded high intensity music (1125 Hz/ 90 dB) through speakers for 5-6 hrs. daily. Blood sampling was done after 20, 40 and 60 days by sacrificing treatment birds. Noise stress significantly (p<0.05) increase the serum levels of corticosterone and thyroid stimulating hormone (TSH) in Group 2 while significantly (p<0.05) decrease the serum levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH) of Group 3 birds. Moreover, major fault bars formation was seen both in Group 2 and Group 3. It was concluded as that Noise stress caused rise in serum levels of Corticosterone and TSH but fall in LH and FSH. Along with fault bars formation was also prominent in all treatment groups due to stress hormone. animals animal s habitat 6 which 80100 80 100 (80-10 g viz temperature twentyfour, twentyfour twenty four, four twenty-four ( group. group . group) season 1 2, , 2) 4. 4 4) 1125 (112 Hz 9 dB 56 5 5- hrs 20 p<0.05 p005 p 0 05 (p<0.05 (TSH (LH FSH (FSH Moreover 8010 8 10 (80-1 112 (11 p<0.0 p00 (p<0.0 801 (80- 11 (1 p<0. p0 (p<0. (80 p<0 (p<0 (8 p< (p< (p

Resumo A poluição ambiental tem o potencial de impactar significativamente a saúde animal, especialmente das aves, devido à exposição diária e ao habitat. Este estudo experimental foi realizado por um período de 60 dias em que, um total de 24 pombos com peso adequado (80-100 g) foram mantidos em biotério com condições ambientais adequadas, ou seja, temperatura, umidade e fonte de luz controladas para minimizar qualquer outro estresse. De 24, 18 aves foram divididas em 3 grupos de tratamento (6 aves em cada grupo). Todo o experimento foi executado em triplicado na época de reprodução. Um deles serviu como controle (Grupo 1) e os 3 restantes foram grupos experimentais, incluindo ruído de tráfego rodoviário (Grupo 2), ruído militar (Grupo 3) e ruído de atividades humanas (Grupo 4). O ruído foi aplicado como música gravada de alta intensidade (1125 Hz/90 dB) através de alto-falantes por 5-6 horas diárias. A coleta de sangue foi feita após 20, 40 e 60 dias sacrificando as aves do tratamento. O estresse sonoro aumentou significativamente (p < 0,05) os níveis séricos de corticosterona e hormônio estimulante da tireoide (TSH) no Grupo 2 enquanto diminuiu significativamente (p < 0,05) os níveis séricos de hormônio luteinizante (LH) e hormônio folículo estimulante (FSH) do Grupo 3. Além disso, a maior formação de barras de falha foi observada tanto no Grupo 2 quanto no Grupo 3. Concluiu-se que o estresse por ruído causou aumento nos níveis séricos de corticosterona e TSH, mas queda em LH e FSH. Junto com a formação de barras de falha também foi proeminente em todos os grupos de tratamento devido ao hormônio do estresse. animal habitat 6 80100 80 100 (80-10 g adequadas seja temperatura 1 ( grupo. grupo . grupo) reprodução experimentais 2, , 2) 4. 4 4) 1125 (112 Hz90 Hz 90 Hz/9 dB altofalantes alto falantes 56 5 5- diárias 20 p 0,05 005 0 05 TSH (TSH (LH FSH (FSH disso Concluiuse Concluiu se 8010 8 10 (80-1 112 (11 Hz9 9 Hz/ 0,0 00 801 (80- 11 (1 0, (80 (8

ABSTRACT. The objective of this study was to evaluate the effect of palm kernel cake and ram effect on reproductive activity of postpartum and lactating ewes. Twenty multiparous ewes, five to eleven days postpartum, were separated into four different treatments: control, control-ram, palm and palm-ram. Body weight, difference in body weight recorded between days 0 and 42 of the experiment, diameter of the largest follicle at day zero of the study, number of ewes bearing a corpus luteum and pregnancy rate were measured in ewes. Average daily weight gain was measured in lambs. There was no effect (p ≥ 0.05) of palm kernel supplementation, ram stimulation, or their interactions on the weights of ewes and lambs. A greater number of ewes with corpus luteum (p < 0.05) were observed in ewes stimulated by rams than those not stimulated. The number of pregnant ewes was not affected by ram effect or palm kernel supplementation (p ≥ 0.05). In conclusion, the use of the ram effect increases the incidence of corpus luteum in postpartum and lactating ewes. In addition, palm kernel cake in the diet does not affect body weight of postpartum ewes and lambs. ABSTRACT treatments control controlram, controlram ram, control-ram palmram. palmram ram. palm-ram 4 experiment lambs p 0.05 005 05 stimulation 0.05. . conclusion addition 0.0 00 0.

Abstract In vitro cell culture is a well-established technique present in numerous laboratories in diverse areas. In reproduction, gametes, embryos, and reproductive tissues, such as the ovary and endometrium, can be cultured. These cultures are essential for embryo development studies, understanding signaling pathways, developing drugs for reproductive diseases, and in vitro embryo production (IVP). Although many culture systems are successful, they still have limitations to overcome. Three-dimensional (3D) culture systems can be close to physiological conditions, allowing greater interaction between cells and cells with the surrounding environment, maintenance of the cells' natural morphology, and expression of genes and proteins such as in vivo. Additionally, three-dimensional culture systems can stimulated extracellular matrix generating responses due to the mechanical force produced. Different techniques can be used to perform 3D culture systems, such as hydrogel matrix, hanging drop, low attachment surface, scaffold, levitation, liquid marble, and 3D printing. These systems demonstrate satisfactory results in follicle culture, allowing the culture from the pre-antral to antral phase, maintaining the follicular morphology, and increasing the development rates of embryos. Here, we review some of the different techniques of 3D culture systems and their applications to the culture of follicles and embryos, bringing new possibilities to the future of assisted reproduction. wellestablished well established areas reproduction gametes embryos tissues endometrium cultured studies pathways diseases IVP. IVP . (IVP) successful overcome Threedimensional Three dimensional D (3D conditions environment morphology vivo Additionally threedimensional three produced drop surface scaffold levitation marble printing preantral pre phase Here (IVP

Abstract This review aims to discuss the main factors involved in the development of early antral follicles until gonadotropin dependence. This follicular phase is characterized by intense proliferation of granulosa cells, formation of a fluid-filled cavity, morphological differentiation of cumulus cells, mural granulosa cells and recruitment of theca cells. The interaction between oocyte, granulosa and theca cells is crucial for follicular growth and hormone production. Growth factors produced by the oocyte, such as growth and differentiation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15), regulate granulosa cell proliferation and differentiation and antral cavity development, as well as stimulate the production of follicle-stimulating hormone (FSH) receptors in granulosa cells. In response to FSH, granulosa cells secrete C-type natriuretic peptide (CNP), which acts through its receptor to increase cyclic guanosine monophosphate (cGMP) production and consequently follicular development. Granulosa cells also produce insulin-like growth factor-1 (IGF-1) and increase aromatase enzyme activity, which results in greater sensitivity to gonadotropins and follicular steroidogenesis. The absence of IGF-1 signaling causes cessation of follicular growth at the early antral stage. Many other local factors are involved in the regulation of follicular development. Therefore, this review brings relevant data for a better understanding of the mechanisms involved in the control of early antral follicle growth, emphasizing the role of endocrine and paracrine factors, the oocyte-granulosa cell interaction and the processes of follicular atresia. The challenges for the establishment of efficient culture systems for in vitro growth of early antral follicles are also discussed. dependence fluidfilled fluid filled oocyte factor9 factor 9 factor- GDF9 GDF (GDF-9 protein15 protein 15 protein-1 BMP15, BMP15 BMP , (BMP-15) folliclestimulating stimulating FSH (FSH Ctype C type CNP, CNP (CNP) cGMP (cGMP insulinlike insulin like factor1 1 IGF1 IGF (IGF-1 activity steroidogenesis IGF- stage Therefore oocytegranulosa atresia discussed (GDF- protein1 protein- BMP1 (BMP-15 (CNP (IGF- (GDF (BMP-1 (IGF (BMP- (BMP

Resumo Esta revisão tem como objetivo discutir os principais fatores envolvidos no desenvolvimento de folículos antrais iniciais até a dependência de gonadotrofinas. Essa fase folicular é caracterizada por intensa proliferação de células da granulosa, formação de uma cavidade preenchida por líquido, diferenciação morfológica das células do cumulus, células da granulosa murais e recrutamento de células da teca. A interação entre oócito, células da granulosa e da teca é determinante para o crescimento folicular e produção hormonal. Fatores de crescimento produzidos pelo oócito, fator de crescimento e diferenciação-9 (GDF-9) e proteína morfogenética óssea-15 (BMP-15), regulam a proliferação e diferenciação de células da granulosa, e o desenvolvimento da cavidade antral, bem como estimulam a produção de receptores do hormônio folículo estimulante (FSH) nas células da granulosa. Em resposta ao FSH, as células da granulosa secretam o peptídeo natriurético tipo C (CNP), que atua através de seu receptor para aumentar a produção de monofosfato de guanosina cíclico (GMPc) e consequentemente o desenvolvimento folicular. As células da granulosa também produzem o fator de crescimento semelhante à insulina 1 (IGF-1) e aumentam a atividade da enzima aromatase, o que resulta em maior sensibilidade às gonadotrofinas e esteroidogênese folicular. A ausência de sinalização do IGF-1 causa cessação do crescimento folicular no início do estágio antral. Muitos outros fatores locais estão envolvidos na regulação do desenvolvimento folicular. Por tanto essa revisão traz dados relevantes para uma melhor compreensão dos mecanismos envolvidos no controle do crescimento de folículos antrais iniciais, enfatizando o papel dos fatores endócrinos e parácrinos, a interação oócito-células da granulosa e os processos de atresia folicular. Os desafios para o estabelecimento de sistemas de cultivo eficientes para o crescimento in vitro de folículos antrais iniciais também são discutidos. líquido cumulus oócito hormonal diferenciação9 9 diferenciação- GDF9 GDF (GDF-9 óssea15 óssea 15 óssea-1 BMP15, BMP15 BMP , (BMP-15) antral FSH (FSH CNP, CNP (CNP) GMPc (GMPc IGF1 IGF (IGF-1 aromatase IGF- parácrinos oócitocélulas discutidos (GDF- óssea1 óssea- BMP1 (BMP-15 (CNP (IGF- (GDF (BMP-1 (IGF (BMP- (BMP

Abstract The objective of this study was to evaluate the effect of three doses of equine chorionic gonadotropin (eCG) for ovarian superstimulation on ovarian response, follicular development and cumulus-oocyte complexes (COCs) collection in llamas. For this purpose, eighteen multiparous non-lactating adult (4–7 yo) female llamas with an average body condition of 2.8 (BCS 1–5) were submitted to a follicular ablation (FA) to induce a new follicular wave emergence. Two days after FA (Day 0), synchronized llamas were randomly allocated to three treatment groups (n = 6/group) and given 500, 750 and 1000 IU of eCG (Novormon®, Syntex, Buenos Aires, Argentina) per animal respectively to induce ovarian superstimulation. Transrectal ultrasonography were performed on Days 2, 4, and 6; and ovum pick up (OPU) was performed on Day 6. Data was evaluated by one-way analysis of variance (ANOVA), repeated measures ANOVA, and 2-tailed Chi-square. The average size (mm) of follicles was greater (p≤ 0.05) in the 1000 IU group compared to the other groups. There was a greater (p≤ 0.05) number of follicles ≥ 7 mm in the 1000 IU group compared to the 500 IU group. Number of COCs collected on Day 6 and the COC recovery rate were not different among groups. In conclusion, a single dose of 1000 IU of eCG induced the best ovarian response resulting in larger and greater number of follicles at the time of OPU. (eCG cumulusoocyte cumulus oocyte (COCs purpose nonlactating non lactating 4–7 47 4 (4– yo 28 2 8 2. BCS 1–5 15 1 5 (FA emergence 0, 0 , 0) n 6/group 6group 75 100 Novormon®, Novormon (Novormon® Syntex Aires Argentina OPU (OPU oneway one way ANOVA (ANOVA) 2tailed tailed Chisquare. Chisquare Chi square. square Chi-square (mm p≤ p (p 0.05 005 05 50 conclusion 4– (4 1– 10 Novormon® (Novormon (ANOVA 0.0 00 ( 0.

ABSTRACT Purpose: Osteoporosis is a bone disease which commonly occurred in postmenopausal women. Almost 10 percent of world population and approximately 30% of women (postmenopausal) suffer from this disease. Alternative medicine has great success in the treatment of osteoporosis disease. Bryodulcosigenin, a potent phytoconstituent, already displayed the anti-inflammatory and antioxidant effect. In this study, we made effort to analyze the antiosteoporosis effect of bryodulcosigenin against ovariectomy (OVX) induced osteoporosis in rats. Methods: Swiss albino Wistar rats were grouped into fIve groups and given an oral dose of bryodulcosigenin (10, 20 and 30 mg/kg) for eight weeks. Body weight, uterus, bone mineral density, cytokines, hormones parameters, transforming growth factor (TGF)-β, insulin-like growth factor (IGF), osteoprotegerin (OPG), receptor activator of nuclear factor kappa-Β ligand (RANKL), and its ratio were estimated. Results: Bryodulcosigenin significantly (p < 0.001) suppressed the body weight and enhanced the uterine weight and significantly (p < 0.001) increased the bone mineral density in whole femur, caput femoris, distal femur and proximal femur. Bryodulcosigenin significantly (P < 0.001) altered the level of biochemical parameters at dose dependent manner, significantly (P < 0.001) improved the level of estrogen and suppressed the level of follicle stimulating hormone and luteinizing hormone. Bryodulcosigenin significantly (P < 0.001) improved the level of OPG and suppressed the level of RANKL. Conclusions: Bryodulcosigenin reduced the cytokines level and suppressed the TGF-β and IGF. We concluded that bryodulcosigenin is an antiosteoporosis medication based on the findings. Purpose 1 (postmenopausal phytoconstituent antiinflammatory anti inflammatory study OVX (OVX Methods 10, (10 2 3 mg/kg mgkg mg kg weeks uterus TGFβ, TGFβ TGF β, β (TGF)-β insulinlike insulin like IGF, IGF , (IGF) OPG, (OPG) kappaΒ kappa Β RANKL, RANKL (RANKL) estimated Results p 0.001 0001 0 001 femoris P manner Conclusions findings (1 (IGF (OPG (RANKL 0.00 000 00 ( 0.0 0.

Abstract Steroids and gonadotrophins are essential for the regulation of late stages of preantral development and antral follicular development. Although the luteinizing hormone receptor (LHCGR) has been detected in the preantral follicles of rats, rabbits, and pigs, its expression, in bovine fetal ovary, has not been demonstrated. Based on this, we aimed to investigate the expression of the LHCGR and LHCGR mRNA binding protein (LRBP), as well as, to quantify bta-miR-222 (a regulatory microRNA of the LHCGR gene) during the development of bovine fetal ovary. In summary, LHCGR expression was observed in the preantral follicle in bovine fetal ovary, from oogonias to primordial, primary and secondary stages, and the mRNA abundance was lower on day 150 than day 60. However, the mRNA abundance of LRBP followed the opposite pattern. Similar to LRBP, the abundance of bta-miR-222 was higher on day 150 than day 60 or 90 of gestation. The LHCGR protein was detected in oogonia, primordial, primary, and secondary follicles. Moreover, both oocytes and granulosa cells showed positive immunostaining for LHCGR. In conclusion, we suggest the involvement of LHCGR/LRBP/bta-mir222 with mechanisms related to the development of preantral follicles in cattle. (LHCGR rats rabbits pigs ovary demonstrated this , (LRBP) btamiR222 btamiR bta miR 222 bta-miR-22 a gene summary primordial 15 However pattern 6 9 gestation oogonia Moreover conclusion LHCGR/LRBP/btamir222 LHCGRLRBPbtamir222 LHCGRLRBPbtamir LHCGR/LRBP/bta mir222 mir LHCGR/LRBP/bta-mir22 cattle (LRBP btamiR22 22 bta-miR-2 1 btamir222 btamir LHCGR/LRBP/btamir22 LHCGRLRBPbtamir22 LHCGRLRBPbta mir22 LHCGR/LRBP/bta-mir2 btamiR2 2 bta-miR- btamir22 LHCGR/LRBP/btamir2 LHCGRLRBPbtamir2 mir2 LHCGR/LRBP/bta-mir bta-miR btamir2 LHCGR/LRBP/btamir

Abstract Lipid metabolism is essential for ensuring oocyte maturation and embryo development. β-Oxidized fatty acids (FA) are a potent source of energy for cells, particularly for bovine somatic follicular cells. Superstimulatory protocols using follicle stimulating hormone (FSH) or FSH combined with equine chorionic gonadotropin (eCG) are capable of stimulating the follicular microenvironment and drive the expression of biomarker genes associated with lipid metabolism in the cumulus-oocyte complex (COC) for better embryo development. In this study, we assesed the effects of FSH and FSH/eCG protocols on the expression of genes related to lipid metabolism in bovine granulosa cells (GCs). Further, we measured triglyceride levels in follicular fluid (FF) obtained from both superstimulatd and non-superstimulated cows (synchronized cows). In summary, superstimulation with gonadotropins maintained the TG levels in bovine FF and ensured GCs mRNA abundance of ACSL1, ACSL3, ACSL6, SCD, ELOVL5, ELOVL6, FASN, FADS2, and SREBP1. We, however, found the abundance of CPTIB mRNA to be lower in GCs obtained from cows subjected to FSH/eCG protocols than synchronized cows. In conclusion, the findings of this study showed that ovarian superstimulation around the preovulatory phase has a mild impact on the lipid metabolism in GCs. development βOxidized β Oxidized FA (FA (FSH eCG (eCG cumulusoocyte cumulus COC (COC FSHeCG . (GCs) Further (FF nonsuperstimulated non superstimulated cows) summary ACSL1 ACSL ACSL3 ACSL6 SCD ELOVL5 ELOVL ELOVL6 FASN FADS2 FADS SREBP1 SREBP We however conclusion (GCs

ABSTRACT This exploratory study aimed to investigate the effects of breed and body mass index (BMI) on arrested-laying (AL) associated with polycystic ovarian syndrome (AL-PCOS) in geese. Moreover, detailed pathological assessments in geese with AL were also performed. Mast geese and Large gray geese were reared under intensive feeding conditions. Observations during the laying season showed that all AL cases (n:8) (7.84%) were in Mast geese (n:102) fed with energy and protein-dense feeds. Necropsy revealed that all Mast geese with AL also had PCOS (8/8). In Mast geese, a significant difference was observed in body weight (BW) (p<0.0001) and BMI (p<0.0001) between the AL-PCOS group and the regular laying group. Follicle classifications detected by necropsy in AL-PCOS geese showed that among the Mast geese with AL-PCOS (n=8), two had atretic and cystic follicles (25%), four had type-1 and type-2 follicles (50%), and two had necrotic type-3 follicles (25%). The research data revealed that AL-PCOS might be correlated with breed and BMI, and that overfeeding and high BMI might increase AL- PCOS in breeds with high egg production, such as Mast geese. Therefore, it is crucial for farms raising high-yielding breeds such as Mast geese to strictly follow the laying periods and feeding regimes for high profitability. No previous reports had ever investigated AL-PCOS in geese, making this study the first of its kind. Moreover, the current study is also among the few presenting data on geese reproductive physiology and pathology. (BMI arrestedlaying arrested (AL ALPCOS (AL-PCOS Moreover performed conditions n8 n 8 (n:8 7.84% 784 7 84 (7.84% n102 102 (n:102 proteindense protein dense feeds 8/8. 88 8/8 . (8/8) BW (BW p<0.0001 p00001 p 0 0001 (p<0.0001 n=8, n=8 , (n=8) 25%, 25 25% (25%) type1 type 1 type- type2 2 50%, 50 50% (50%) type3 3 25%. production Therefore highyielding yielding profitability kind pathology (n: 7.84 78 (7.84 n10 10 (n:10 8/ (8/8 p<0.000 p0000 000 (p<0.000 n= (n=8 (25% 5 (50% (n 7.8 (7.8 n1 (n:1 (8/ p<0.00 p000 00 (p<0.00 (n= (25 (50 7. (7. (8 p<0.0 p00 (p<0.0 (2 (5 (7 ( p<0. p0 (p<0. p<0 (p<0 p< (p< (p

ABSTRACT Purpose: XinJiaCongRongTuSiZiWan (XJCRTSZW) is a traditional Chinese medicine compound for invigorating the kidney, nourishing blood, and promoting blood circulation. This study aimed to explore the effect of XJCRTSZW on triptolide (TP)-induced oxidative stress injury. Methods: Adult female Sprague-Dawley rats and human ovarian granulosa cell lines were treated with TP and XJCRTSZW. Hematoxylin and eosin staining, enzyme-linked immunosorbent assay, flow cytometry, CCK-8, JC-1 staining, transmission electron microscopy, reverse transcription-quantitative polymerase chain reaction, and Western blotting were performed in this study. Results: XJCRTSZW treatment observably ameliorated the TP-induced pathological symptoms. Furthermore, XJCRTSZW treatment observably enhanced the TP-induced reduction of estradiol, anti-Mullerian hormone, progesterone, superoxide dismutase, ATP content, mitochondrial membrane potential, p62, and Hsp60 mRNA, and protein levels in vivo and in vitro (p < 0.05). However, TP-induced elevation of follicle stimulating hormone and luteinizing hormone concentrations, malondialdehyde levels, reactive oxygen species levels, apoptosis rate, mitophagy, and the mRNA and protein expressions of LC3-II/LC3-I, PTEN-induced kinase 1 (PINK1), and Parkin were decreased (p < 0.05). In addition, XJCRTSZW treatment markedly increased cell viability in vitro (p < 0.05). Conclusions: XJCRTSZW protects TP-induced rats from oxidative stress injury via the mitophagy-mediated PINK1/Parkin pathway. Purpose (XJCRTSZW kidney circulation TPinduced induced Methods SpragueDawley Sprague Dawley staining enzymelinked enzyme linked assay cytometry CCK8, CCK8 CCK 8, 8 CCK-8 JC1 JC JC- microscopy transcriptionquantitative transcription quantitative reaction Results symptoms Furthermore estradiol antiMullerian anti Mullerian progesterone dismutase content potential p62 p Hsp Hsp6 0.05. 005 0.05 . 0 05 0.05) However concentrations rate mitophagy LC3II/LC3I, LC3IILC3I LCIILCI LC3 II/LC3 I, II I LC LC3-II/LC3-I PTENinduced PTEN PINK1, PINK1 PINK , (PINK1) addition Conclusions mitophagymediated mediated PINK1Parkin PINKParkin pathway CCK- p6 00 0.0 LC3II LC3I LC3II/LC3I IILC IILC3 II/LC (PINK1 0. LCII LCI (PINK

Abstract The number of antral follicles is considered an important fertility trait because animals with a high follicle count (HFC) produce more oocytes and embryos per cycle. Identification of these animals by genetic markers such as single nucleotide polymorphisms (SNPs) can accelerate selection of future generations. The aim of this study was to perform a genome wide association study (GWAS) on Nelore and Angus heifers with HFC and low (LFC) antral follicle counts. The groups HFC and LFC for genotyping were formed based on the average of total follicles (≥ 3 mm) counted in each breed consistently ± standard deviation. A total of 72 Nelore heifers (32 HFC and 40 LFC) and 48 Angus heifers (21 HFC and 27 LFC) were selected and the DNA was extracted from blood and hair bulb. Genotyping was done using the Illumina Bovine HD 770K BeadChip. The GWAS analysis showed 181 and 201 SNPs with genotype/phenotype association (P ≤ 0.01) in Nelore and Angus heifers, respectively. Functional enrichment analysis was performed on candidate genes that were associated with SNPs. A total of 97 genes were associated to the 181 SNPs in the Nelore heifers and the functional analysis identified genes (ROBO1 and SLIT3) in the ROBO-SLIT pathway that can be involved in the control of germ cell migration in the ovary as it is involved in lutheal cell migration and fetal ovary development. In the Angus heifers, 57 genes were associated with the 201 SNPs, highlighting Fribilin 1 (FBN1) gene, involved in regulation of growth factors directly involved in follicle activation and development. In summary, GWAS for Nelore and Angus heifers showed SNPs associated with higher follicle count phenotype. Furthermore, these findings offer valuable insights for the further investigation of potential mechanism involved in follicle formation and development, important for breeding programs for both breeds. (HFC cycle (SNPs generations (GWAS (LFC counts ≥ ( mm deviation 7 32 (3 4 21 (2 2 bulb K BeadChip 18 20 genotypephenotype genotype phenotype P 0.01 001 0 01 respectively 9 ROBO1 ROBO (ROBO SLIT3 SLIT ROBOSLIT development 5 FBN1 FBN (FBN1 gene summary Furthermore breeds 0.0 00 (FBN 0.