RESUMEN Colombia es el noveno productor de pitahaya amarilla en el mundo y el Departamento de Boyacá es el mayor productor con 440 hectáreas plantadas. Los fitopatógenos bacterianos pueden causar pérdidas de frutos de pitahaya y bajos rendimientos al producir la pudrición blanda de los tallos. El objetivo de esta investigación fue estudiar e identificar posibles agentes de enfermedades bacterianas de la pitahaya en Boyacá, Colombia. Trece (13) fincas de la región fueron seleccionadas para la toma de 20 muestras mediante un muestreo dirigido y al azar de tejidos de tallo y frutos con síntomas de pudrición blanda en estadios iniciales de la enfermedad. Se realizó un proceso de aislamiento microbiológico, identificación bioquímica y taxonómica molecular de las bacterias aisladas. Las secuencias del gen ARNr 16s de la región V2-V5 fueron editadas mediante la eliminación de los primers, ensamblaje y obtención de la secuencia consenso de los primers 1100R-337F y 800R -518F. El análisis filogenético fue hecho por BLAST en el NCBI, por las herramientas "Classifier" y "SeqMatch", del sitio Web del RDP y se realizaron árboles filogenéticos mediante alineamiento múltiple usando el algoritmo MUSCLE y el método de distancias de Tamura Nei. Se registraron signos como ampollas y fluidos mucilaginosos, síntomas como manchas cloróticas amarillas y marrones, pudrición blanda y licuefacción, y se reportaron posibles vectores como moscas y hormigas. Veinticinco (25) morfotipos de bacterias fueron clasificados en 4 filos, 8 familias y 13 géneros. El análisis de las secuencias del gen ARNr 16S de las cepas bacterianas mostró una identidad del 98 al 100% con Enterobacter cloacae, Pectobacterium carotovora y Paenibacillus glucanolyticus, reportados en otros estudios como causantes de la pudrición blanda del tallo y fruto de pitahaya. Se reportaron nuevas especies como posibles bacterias patógenas de pitahaya: Pantoea cypripedii, Kluyvera intermedia y Klebsiella oxytoca. Los ensayos de infectividad no arrojaron resultados positivos. La microbiota identificada en los estadios de la fase necrotrofa o final de la pudrición blanda del tallo podría estar conformada por los géneros Achromobacter, Sphingobacterium, Pseudomonas, Klebsiella, Paenibacillus, Bacillus, Stenotrophomonas y Microbacterium. En la mosca Leptoglossus zonatus asociada al cultivo se identificó Pseudomonas fulva y Lysinibacillus fusiformis. Este es el primer registro oficial de un complejo de posibles bacterias fitopatógenas del orden Enterobacterales (familia Enterobacteriaceae, Erwiniaceae y Pectobacteriaceae) en síntomas por enfermedad bacteriana en cultivos colombianos de pitahaya. La identificación de las bacterias de un pato-sistema puede orientar las prácticas de control químico y biológico con el fin de incrementar el potencial productivo y de exportación de los cultivos exóticos y huérfanos de las pequeñas fincas locales.

ABSTRACT Colombia is the ninth largest producer of dragon fruit in the world and the department of Boyacá is the largest with 440 hectares planted. Bacterial phytopathogens can cause pitahaya fruit losses and low yields by producing stem soft rot. This research aimed to study and identified possible agents of bacterial diseases of pitahaya in Boyacá, Colombia. Thirteen farms in the region were selected to take 20 samples by means of a targeted and random sampling of stem and fruit tissues with soft rot symptoms in the early stages of the disease. A process of microbiological isolation, biochemical and molecular taxonomic identification of the isolated bacteria. The 16s rRNA gene sequences of the V2-V5 region were edited by removing the primers, assembling and obtaining the consensus sequence of the primers 1100R-337F and 800R -518F. The phylogenetic analysis was performed by BLAST at NCBI, using the "Classifier" and "SeqMatch" tools from the RDP website, and phylogenetic trees were created by multiple alignment using the MUSCLE algorithm and the Tamura Nei distance method was performed. Signs like blisters and mucilaginous fluids, symptoms like yellow and brown chlorotic spots, soft rot, and liquefaction were identified, and possible vectors like flies and ants were report. Twenty-five bacterial morphotypes were identified classifieds in 4 phyla, 9 families and 13 genera. The analysis of the 16S rRNA gene sequences of the bacterial strains showed a 98 to 100% identity with Enterobacter cloacae, Pectobacterium carotovora and Paenibacillus glucanolyticus, reported in other studies as causing the soft rot of the pitahaya stem and fruit. New species were reported as possible pathogenic bacteria of pitahaya: Pantoea cypripedii, Kluyvera intermedia and Klebsiella oxytoca. Infectivity assays did not have positive results. The microbiota identified in the stages of the necrotrophic phase or final stage of soft rot of the stem belong to the genera Achromobacter, Sphingobacterium, Pseudomonas, Klebsiella, Paenibacillus, Bacillus, Stenotrophomonas and Microbacterium. In the fly Leptoglossus zonatus associated with the crop were identified Pseudomonas fulva and Lysinibacillus fusiformis were identified. This is also the first official report of a complex of possible phytopathogen bacteria of the order Enterobacterales (Enterobacteriacea, Erwiniaceae and Pectobacteriaceae family) in symptoms by bacterial disease on pitahaya Colombian crops. Identification of the bacteria in a pathogenic system can guide chemical and biological control practices in order to increase the productive and export potential of exotic and orphan crops from small local farmings.

Abstract Introduction: Hermetia illucens is a fly found worldwide in tropical and temperate regions that feeds on decaying organic matter in its larval stage, which makes them useful to accelerate composing processes. Bacterial component on the larval guts that helps to degrade organic matter is well studied, however fungal communities information is more scarse, specially in tropical regions. Objective: To determine fungal communities in the gut of H. illucens larvae naturally occurring during urban composting processes in a tropical region. Methods: ITS sequencing was employed to characterize fungal communities present in H. illucens larval gut. Results: The analysis of amplicon sequence variants (ASVs) unveiled a notable dominance of Saccharomycetales, being yeasts of the genus Pichia the most abundant. Other relatively abundant yeasts were Candida and Galactomyces and the genus Archaeospora from Archaeosporales. The last two groups not being reported in H. illucens before. Conclusions: Yeasts of the genus Pichia are the most abundant group, this result is in concordance with previous studies which suggest a stable insect-yeast relation. The description of previously unreported fungal groups highlights the importance of continuing to explore the mycobiome dynamics of this larva. This study offers insight into the mycobiome of naturally occurring H. illucens larvae in a tropical region.

Resumen Introducción: Hermetia illucens se distribuye mundialmente en regiones tropicales y templadas. En su estado larval se alimenta de materia orgánica en descomposición, lo que las hace útiles para acelerar los procesos de compostaje. Se han realizado diversos estudios sobre el componente bacteriano de los intestinos de las larvas que ayuda a degradar la materia orgánica, sin embargo, información sobre las comunidades de microhongos es más escasa, especialmente en regiones tropicales. Objetivo: Determinar el micobioma presente en los intestinos de las larvas de H. illucens que se desarrollaron naturalmente en compostajes urbanos en una región tropical. Métodos: Se realizó secuenciación ITS para caracterizar las comunidades de microhongos presentes en el intestino de las larvas. Resultados: El análisis de variantes de secuencia de amplicones (ASV) reveló una notable dominancia de Saccharomycetales, siendo las levaduras del género Pichia las más abundantes. Otras levaduras relativamente abundantes fueron Candida y Galactomyces y el género Archaeospora de Archaeosporales. Los dos últimos grupos no han sido reportados previamente en H. illucens. Conclusión: Determinamos que las levaduras del género Pichia son el grupo más abundante. Este resultado concuerda con estudios previos, lo que sugiere una relación estable entre estos dos organismos. La descripción de grupos de hongos no reportados previamente resalta la importancia de continuar explorando la dinámica del micobioma de esta larva. Este estudio proporciona información sobre el micobioma de las larvas de H. illucens que se presentan naturalmente en una región tropical.

Abstract Introduction: The West Indian fruit fly, Anastrepha obliqua (Macquart), is one of the most economically important pests of mangoes in Mexico and the Neotropics. Objective: To analyze adult population patterns of A. obliqua and their relation to local and landscape factors. Methods: We selected 11 “Manila” mango orchards along the middle part of La Antigua River, which were characterized to determine their biophysical structure and landscape configuration considering their distance to six different land uses/land covers. Anastrepha obliqua population was estimated by collecting adult flies in colorless polyethylene plastic bottle traps baited with a protein for three weeks in June 2022. Results: A total of 1 869 adults of A. obliqua were trapped, of which 75 % were females and 25 % were males. Abundance varied from 68 to 490 adult flies per orchard, while capture frequency from 0.36 to 1.8 flies/trap/day. Abundance increased in highly shaded orchards, and capture frequency decreased in orchards with highly soil compaction. Abundance and the frequency of capture increased in orchards near isolated trees of Spondias spp. and decreased in orchards near sugarcane plantations. Shade management in mango orchards may be used as an effective strategy to promote biotic interactions to naturally regulate A. obliqua populations, meanwhile, soil compaction represents a limiting ecological condition, which might severely impact fruit fly larvae survival. Conclusions: Adult population of A. obliqua depends on several local and landscape factors because they indicate resource availability and ecological conditions. These findings could be considered for control strategies in the integrated management of this pest to promote protection and improve the fruit quality of mango crop.

Resumen Introducción: La mosca de las indias occidentales, Anastrepha obliqua (Macquart), es una de las plagas más importantes económicamente en el cultivo de mango de México y el neotrópico. Objetivo: Analizamos los patrones de la población adulta de A. obliqua y su relación con factores locales y del paisaje. Métodos: Un total de 11 huertos de mango “Manila” fueron caracterizados en estructura biofísica y configuración del paisaje. Los adultos de A. obliqua fueron capturados con trampas (botellas de polietileno cebadas con una proteína) durante tres semanas en junio de 2022. Resultados: Se capturaron 1 869 adultos de A. obliqua, de los cuales 75 % fueron hembras y 25 % machos. La abundancia varió de 68 a 490 moscas adultas por huerto, mientras que la frecuencia de captura de 0.36 a 1.8 moscas/trampa/día. La abundancia incrementó en huertos altamente sombreados y decreció en huertos con suelos compactos. La abundancia y frecuencia incrementaron en huertos cercanos a árboles de Spondias spp. y decreció en huertos cercanos a plantaciones de caña de azúcar. El manejo de la sombra puede ser utilizado como una estrategia efectiva para promover las interacciones que regulan naturalmente la población de A. obliqua, mientras que la compactación del suelo representa una condición ecológica limitante que podría impactar severamente en la supervivencia de larvas de moscas de la fruta. Conclusión: La población adulta de A. obliqua depende de varios factores locales y del paisaje que indican disponibilidad de recursos y condiciones ecológicas. Estos hallazgos podrían considerarse en las estrategias del manejo integrado de esta plaga para promover la protección y el mejoramiento de la calidad de frutos en el cultivo de mango.

The leaching kinetics of cerium (Ce) and yttrium (Y) in sodium-hydroxide-digested non-magnetic coal fly ash using acetic acid were investigated. The variables analysed were pH, solid-to-liquid ratio (S/L), and temperature. The results show that cerium and yttrium's maximum recovery was 57.33% and 58.36%, respectively, under leaching temperature of 90°C for Ce and 60°C for Y at pH of 1,74 and 10 g/mL solid-liquid ratio in 240 minutes. Increasing the leaching time to more than 120 minutes did not significantly improve the results. After the magnetic separation process, the ICP-OES analysis shows a slight increase in the concentration of cerium and yttrium on nonmagnetic components. The XRD and ICP-OES analysis for the sample before and after silicate digestion shows that 40% silicate was dissolved. The calculation of apparent activation energies of leaching using the Arrhenius law for the leaching of cerium and yttrium gives 22.72 kJ/mol and 35.84 kJ/mol, respectively. The kinetic leaching model of cerium and yttrium all fit Dickinson's new shrinking core model. The calculation results show that the leaching reaction is controlled by combining interface transfer and diffusion across the product layer.

Hierdie sewe-jaar longitudinale opvoedingstudie evalueer drie akademiese afleweringsmodusse vir deeltydse MBA-studente in hul Bemarkingsbestuursmodule, naamlik vol kontak (20172019), vol aanlyn tydens COVID-19 se harde inperking (2020-2021 en die daaropvolgende gemengde modus (2022-2023). Die studie het ten doel om te bepaal of die afleweringsmodus studente se akademiese prestasie beduidend beïnvloed. Die eenrigting-variansie-analise (EVA) het beduidende verskille tussen die afleweringsmodusse vir individuele opdragte (normatiewe assessering) en groepgevallestudies (summatiewe assessering) geïdentifiseer. Daar was ook betekenisvolle verskille tussen die akademiese prestasie ten opsigte van die finale saamgestelde portefeulje van kennis (wat as eksamen dien). Hierdie portefeulje bestaan uit 'n prakties-toegepaste bemarkingsplan. Die post-hoc toetse het getoon dat studente aansienlik beter gevaar het tydens die gemengde afleweringsmodus in individuele opdragte, terwyl die volledige aanlyn modus laer punte vir die groepgevallestudies gehad het. Beide die gemengde en volledige kontakmodusse het beduidende opeenvolgende verbetering vir die groepgevallestudies getoon, wat in elke gevallestudie verbetering getoon het namate die semester gevorder het. Dit verskil van volledige aanlyn onderrig. Die gemengde modus het ook aansienlik beter gevaar in die eksamen- en finale modulepunte en het selfs beter gevaar as volkontakklasse.

The COVID-19 pandemic significantly transformed the tertiary education landscape. Traditional contact universities were forced abruptly to adopt a full-distance education mode, a situation neither the students nor lecturers were prepared for. Many lectures and teaching activities were developed on the fly as universities struggled to roll out the needed support in double-time for lecturers and students. This new normal for education required integrating technology, advanced communication software, revised assessment techniques, and recognising the need for flexible and adaptable approaches to teaching and learning. Students also evolved and gained technological proficiency, adaptability, time management, soft skills, social media proficiency, and an awareness of health and well-being. Albeit uncertain in redefining the new normal, universities continue to provide quality education. However, exactly how university education should be delivered to a new breed of students in a new business environment is a challenge. This is even more so in the case of executive MBA students. After the hard lockdown and restrictions were lifted on 21 September 2020, the North-West University Business School started to explore other modes of academic content delivery. Reinstating a full contact delivery mode was not an option because of remaining restrictions. High technological investment, online-educated lecturers and students, and benefits and developments during online learning now form a new normal for the Business School. Which teaching method, or rather, which mode of delivery, is the most relevant and adequate? Should the MBA lectures return to the historic full-contact delivery mode, stick with the newly established full online mode, or attempt to integrate the benefits of both into a hybrid mode of delivering business education to a new breed of technology-savvy students? This study, therefore, examines the academic performance of the different delivery modes by comparing data of Executive MBA Marketing management students at the NWU Business School in South Africa. The study examines the academic performance of these students from 2017 up to 2023. The panel database consists of Executive MBA students'marks for the module in Marketing Management from 2017 up to 2023. The database contains marks for individual assignments, groupwork case studies, final individual- and groupwork marks, exam marks and the final module mark. The final assessment is a portfolio of evidence comprising a detailed marketing plan for a product, service, or organisation of choice. The mode of delivery served as the dependent variable, while the independent variables comprised the marks for each assignment (groupwork, individual and portfolio of evidence). Three delivery modes were compared, namely full-contact (2017-2019), full-online (2020-2021), and the post-pandemic hybrid mode currently in effect (2022-2023). The data comprised 630 cases. However, the analysis of variance (ANOVA) used 532 cases to compare the different groupwork assignments (230 full-contact students, 159 full-online students, and 143 hybrid students). Cronbach's alpha coefficient measured the data's reliability, and one-way ANOVA determined significant differences (p<.05) between the academic performance of the three delivery modes. The analysis confirmed that significant differences exist between the modes of delivery for individual assignments, group case studies and exams (p<.05). The Tukey ab post-hoc tests showed that students performed significantly better during the hybrid delivery mode in individual assignments (69.2%) than full contact (63.8%). Regarding the individual assignment, the full online (63.8%) and full contact (63.7%) delivery modes perform significantly worse than the hybrid (69.2%) delivery mode. Regarding the first group case study (Group Assignment 1), full online performs (67.3%) significantly worse than hybrid (71.1%) and full contact (73.6%) delivery modes. There is no significant difference between the hybrid and full contact delivery modes. In the second, third, and fourth group case studies (Group Assignments 2, 3 & 4), the hybrid delivery mode (82.37%, 75.4%, 82.7%) outperforms the full contact (75.4, 80.1%, 86.6%) and full-online (71.4%, 71.4%, 67.8%) modes. The hybrid delivery mode, therefore, proves to be more conducive towards academic performance in group work. Finally, in considering the final module mark, the hybrid delivery mode (71.0%) is significantly higher than the other two delivery modes. However, there is no significant difference between full online (67.5%) and full-contact (67.2%) delivery modes regarding the final module mark. Nevertheless, it is noteworthy that the better performance in the final module marks is a function of the groupwork where the students analysed case studies together. There are no significant differences in the exam marks between the delivery modes, so better academic performance in the hybrid mode of delivery where groupwork is concerned culminates in the better module mark. Based on the findings, it is evident that the hybrid mode of delivery results in the best academic performance. This observation is based on three reasons, namely 1) students performed better in both the normative and summative assessments during hybrid tuition; 2) there is continuous progressive learning throughout the semester regarding the group case studies (summative assessment). They learn to improve and hone their skills to analyse better and apply business solutions to real-life problems experienced by organisations. This is based on students' improving marks in the group case studies as they become more proficient in analysing and answering the applied case studies because they learn from each case study; and 3) the students perform better academically in their normative assessments and earn higher final module marks (partially due to reason 2). It is concluded that the hybrid delivery mode facilitates the academic performance of post-pandemic executive MBA Marketing management students. JEL KODES: I23, I21, M30

Resumen: En la actualidad, existen materiales provenientes de la industria, que bajo ciertas condiciones físicas pueden aportar al mejoramiento de las propiedades de mecánicas de un suelo. Materiales como el polvo de ladrillo (BD) y las cenizas de carbón (FA) tienen contenidos altos de SiO2 y Al3O2, que denotan actividad puzolánica. Además, se ha comprobado que dichos materiales pueden activarse al combinarse con cal. Esto genera procesos internos de cementación cuando el tamaño de grano es de 75 micras. Las vías rurales en Colombia presentan uno de los porcentajes más altos de toda la red vial y solo un 7% aproximadamente se encuentra en buen estado. Las difíciles condiciones de acceso, la susceptibilidad de los suelos, la imposibilidad financiera de intervenir toda esta red y la necesidad de implementar procesos de economía circular hace que estos materiales sean atractivos en temas de estabilización para mejorar las condiciones de tránsito. El BD y FA fueron aplicadas en dosificaciones del 0%, 3%, 6%, 9%, 12% y 24% en suelos finogranulares (limo y arcilla) y suelos arenosos, se evaluó la compactación y se realizó un diseño experimental factorial para evaluar la influencia del material en la variable resistencia a la compresión inconfinada, por medio de un análisis ANOVA. Para evaluar el desempeño del BD y las FA, se realizó una pista de prueba sobre una vía de bajo volumen de tráfico en el norte de Colombia, la cual tenía un suelo arenoso. BD y FA fueron adicionados al 12% y activados con cal, en celdas de 30 m de longitud. Para establecer un patrón comparativo se hicieron otras celdas en las mismas condiciones geométricas con materiales que usualmente se usan en este tipo de aplicaciones como el cemento. Estas celdas fueron evaluadas en periodo de 16 meses. Características como el módulo resiliente, El índice de internacional de rugosidad IRI y el coeficiente de resistencia al deslizamiento fueron medidos en ese período. Los resultados indican que cuando se adicionan estos materiales en suelos finogranulares (limos y arcillas), la resistencia a la compresión inconfinada aumenta un 150% respecto al suelo sin estabilizar, mientras que para suelos arenosos la resistencia aumenta desde un 70% hasta un 125%. Durante el período de evaluación, el BD y las FA lograron presentar aumentos por encima del 50% en el módulo resiliente respecto al suelo sin estabilizar. Sin embargo, las FA presentaron resultados comparables respecto al tramo estabilizado con cemento. Adicionalmente, sin bien, los tramos se fueron deteriorando en el tiempo, mantuvieron su indicen de rugosidad dentro de los rangos admisibles indicativos de buen índice de serviciabilidad.

Abstract: Currently, there are materials from industry that, under certain physical conditions, can contribute to the improvement of soils mechanical properties. Materials such as brick dust (BD) and fly ash (FA) have high SiO2 and Al2O3 contents, which denote pozzolanic activity. In addition, it has been shown that these materials can be activated when combined with lime. This generates internal cementation processes when the particle size is 0.075 mm. Rural roads in Colombia have one of the highest percentages of the entire road infrastructure, and only about 7% are in good condition. Difficult access conditions, soil susceptibility, the financial impossibility of intervening in this entire network and the need to implement circular economy processes, make these materials attractive in terms of stabilization to improve traffic conditions. BD and FA were applied in dosages of 0%, 3%, 6%, 9%, 12% and 24% in finogranular soils (silt and clay) and sandy soils, compaction was evaluated, and a factorial experimental design was carried out to evaluate the influence of the material on the variable unconfined compressive strength (UCS), through an ANOVA analysis. To evaluate the performance of BD and FA, a test track was made on a low traffic volume road in northern Colombia, which had a sandy soil. BD and FA were added at 12% and activated with lime, in 30 m long cells. To establish a comparative pattern, other cells were made in the same geometric conditions with materials that are usually used in this type of application, such as cement. These cells were evaluated over a period of 16 months. Characteristics such as resilient modulus, international roughness index (IRI) and slip resistance coefficient were measured during this period. The results indicate that when these materials are added to finogranular soils (silts and clays), the UCS increases by 150% with respect to the unstabilized soil, while for sandy soils the strength increases from 70% to 125%. During the evaluation period, the BD and the FA were able to increases of over 50% in the resilient modulus with respect to the unstabilized soil. However, the FA showed comparable results with respect to the cement-stabilized cell. In addition, although the sections deteriorated over time, they maintained their roughness index within the admissible ranges indicative of a good serviceability index.

The decomposition of a body, and the associated gaseous and liquid discharges emanating from it, attract gravid female blow flies which lay their eggs in or on the body. After the eggs have hatched, the emerging larvae start feeding on the body. As decomposition progresses, the blow fly larvae often migrate away, typically in a random manner in search of favourable conditions for pupation. In this paper, we report on a rarely described phenomenon of unidirectional mass migration of blow fly larvae and postulate on the factors which may drive this process. A decomposition trial utilising a 60-kg pig carcass, deployed in the summer months in Table Mountain National Park, Cape Town, South Africa, was conducted in 2022. On the fifth day of the trial, simultaneous unidirectional mass dispersal of blow fly larvae was observed. The larvae moved downhill in a southeasterly direction, following the flow of decomposition fluids oozing out from the pig carcass. The 'larval migration stream' had a length of approximately 1.5 m with a width of 40 cm, tapering to 17 cm at the terminal point. The larval migration stream consisted of the larvae of Chrysomya albiceps and Chrysomya chloropyga. This study demonstrates the importance of understanding the timing and pattern of dispersal of post-feeding blow fly larvae in each geographical region. This is crucial as the minimum post-mortem interval can be miscalculated if older immature insects dispersing from the corpse are not considered and collected during crime scene investigations. SIGNIFICANCE: • Understanding the timing and pattern of dispersal of blow fly larvae from decomposing vertebrate remains in each geographical region is important in time-since-death estimations when using entomological evidence. • In the summer season of the Western Cape Province, Chrysomya albiceps and Chrysomya chloropyga are among the blow fly species of forensic importance as they deposit their eggs on vertebrate remains. • When a decomposing body is found within the Table Mountain region, we suggest that crime scene investigators should examine the downhill area of the scene for larval/pupal entomological evidence that can assist in time and season of death estimations.

Abstract The biological control in sugarcane in Venezuela has been established as a unique measure, having optimal results of the use of parasitoids and entomopathogens for the control of borers of the genus Diatraea, and later of the candelilla Aeneolamia spp., both key pests of the crop. For borers it was achieved after systematic releases of the Amazonian fly Lydella minense from the middle of the last century and then with the establishment of the braconid wasp Cotesia flavipes from 1987. For the biological control of Aenolamia varia, pest that involved the frequent application of insecticides, after the introduction of the use of the entomopathogenic fungus Metarhizium anisopliae since 1977, has been established as an efficient measure for the control of its adults, however, the nymphs were less controlled. This aspect was resolved through the introduction of the entomopathogenic nematode Heterorhabditis bacteriophora in 2004, which has been widely used as a biological measure to avoid the application of insecticides. As an example, we have the Azucarera Río Turbio, although in the last harvests it has had a reduction in its areas due to different factors. During the last 58 years, Diatraea infestation shows a reduction of 15 to 3.18 %, which saves 170 552.62 metric tons with a value of US$ 49 196 045 and a cost of biological control of US$ 1 187 189 which translates into a benefit-cost of US$ 41.44. Before the establishment of biological control, Aeneolamia was controlled exclusively with chemical insecticides applied to foliage for adults and soil for nymphs. Simultaneously since then, the entomopathogenic fungus M. anisopliae produced by national companies has been used and this entomopathogen was used on 87 000 hectares between 1986 and 1990. Also since 2004, the methodology of using entomopathogenic nematodes to control Aeneolamia nymphs began.

Resumen El control biológico en caña de azúcar en Venezuela se ha logrado establecer como una medida única, con resultados óptimos del uso de parasitoides y entomopatógenos para el control de los taladradores del género Diatraea, y, posteriormente, de la candelilla Aeneolamia spp., ambas plagas claves del cultivo. Para los taladradores, se logró después de liberaciones sistemáticas de la mosca amazónica Lydella minense desde un inicio, a mediados del siglo XX y luego con el establecimiento de la avispa bracónida Cotesia flavipes, a partir de 1987. Para el control biológico de Aeneolamia varia, plaga que implicaba la aplicación frecuente de insecticidas, después de la introducción del uso del hongo entomopatógeno Metarhizium anisopliae desde 1977, se ha establecido como medida eficiente para el control de sus adultos, aunque las ninfas eran menos controladas. Este aspecto se resolvió mediante la introducción del nematodo entomopatógeno Heterorhabditis bacteriophora en 2004, el cual se viene utilizando en forma general, y prácticamente ya se tienen las medidas biológicas para evitar la aplicación de insecticidas. Como ejemplo se tiene a la Azucarera Río Turbio, aunque en las últimas zafras ha tenido una reducción en sus áreas, por diferentes factores. Durante los últimos 58 años, la infestación de Diatraea muestra una reducción de 15 a 3.18 %, con lo cual se salvan 170 552.62 toneladas métricas (TM), con un valor de US$ 49 196 045 y un costo del control biológico de US$ 1 187 189 lo que se traduce en un beneficio-costo de US$ 41.44. Antes del establecimiento del control biológico, Aeneolamia se controlaba exclusivamente con insecticidas químicos, aplicados al follaje para los adultos y al suelo para las ninfas. Simultáneamente desde entonces, se ha utilizado el hongo M. anisopliae producido por empresas nacionales, hasta cubrir 87 000 ha, entre 1986 y 1990. También, desde 2004 se inició la utilización de nematodos entomopatógenos para el control de ninfas de Aeneolamia.

Abstract L-ascorbic acid is a natural antioxidant produced by plants and their fruits. The abundance of this acid during the development and postharvest ripening of mangoes (Mangifera indica L.) depends on genetic and climatic factors. The mangoes undergo a quarantine hydrothermal treatment before being exported to control the fruit fly. A quarantine hydrothermal treatment consists of immersing mangoes in water at 46.1 °C (118 °F) for 60 to 120 min depending on the size, affecting the L-ascorbic acid content and other quality parameters of the fruit. This research aimed to evaluate the effect of QHT on L-ascorbic acid content, firmness, color, and expression of L-ascorbic acid biosynthesis and recycling genes during mango postharvest. ‘Ataulfo’ mangoes were harvested at physiological ripeness in Escuinapa, Sinaloa in 2019, subjected to QHT (46.1 °C, 75 min), hydrocooled (25 °C, 30 min) and stored at 20 °C for nine days. Mangoes subjected to QHT showed a reduction in L-ascorbic acid content (p≤ 0.05). The firmness of the pulp behaved similarly in fruits with QHT and in control fruits, while the external color of the fruits with a quarantine hydrothermal treatment indicated an advanced ripening process compared to the control fruits (p≤ 0.05). The levels of MiGME1, MiGME2, MiGGP2, and MiMDHAR transcripts increased in response to treatment, suggesting activation of the synthesis and recycling pathway to counteract the abiotic stress caused by the heat to which the mangoes were subjected.

Resumen El ácido L-ascórbico, es un antioxidante natural producido por las plantas y sus frutos. La abundancia de este ácido durante el desarrollo y la maduración poscosecha del mango (Mangifera indica L.) depende de factores genéticos y climáticos. Los mangos se someten a un tratamiento hidrotérmico cuarentenario, antes de su exportación para control de la mosca de la fruta. El un tratamiento hidrotérmico cuarentenario, consiste en sumergir los mangos en agua a 46.1 °C (118 °F) durante 60 a 120 min dependiendo del tamaño, afectando el contenido de ácido L-ascórbico y otros parámetros de calidad del fruto. El objetivo de esta investigación fue evaluar el efecto del THC en el contenido de ácido L-ascórbico, la firmeza, el color y la expresión de genes de biosíntesis y reciclaje de ácido L-ascórbico durante la postcosecha del mango. Los mangos ‘Ataulfo’ se cosecharon en madurez fisiológica en Escuinapa, Sinaloa en el año 2019, se sometieron a THC (46.1 °C, 75 min), hidroenfriado (25 °C, 30 min) y se almacenaron a 20 °C por nueve días. Los mangos sometidos al THC mostraron reducción en el contenido de ácido L-ascórbico (p≤ 0.05). La firmeza de la pulpa se comportó similar en frutos con THC y en frutos testigo, mientras que el color externo de los frutos con un tratamiento hidrotérmico cuarentenario e indicó un proceso de maduración adelantado respecto a los frutos testigo (p≤ 0.05). Los niveles de transcritos MiGME1, MiGME2, MiGGP2 y MiMDHAR aumentaron en respuesta al tratamiento, lo que sugiere la activación de la ruta de síntesis y reciclaje para contrarrestar el estrés abiótico ocasionado por el calor al que se sometieron los mangos.

Abstract 20. The growth of the aquaculture industry has been driven by the increasing global demand for food and concerns about the overexploitation of natural resources and environmental impact. To address these challenges, aquaculture has sought sustainable and innovative alternatives. In this context, the inclusion of insects, such as the Black Soldier Fly (Hermetia illucens), in the diet of fish and crustaceans emerges as a promising solution due to their high protein, lipid, and mineral content. This review article discusses the role of Soldier Fly meal in aquaculture. It examines the economic, nutritional, and environmental benefits of meal in aquatic animal diets, as well as recent research results on proper dosage, growth quality, gut health, and nutrient availability in various aquaculture species. It is emphasized that Black Soldier Fly meal offers nutritional benefits comparable to fish meal, being a rich source of protein and lipids, and its inclusion in the diet of aquatic species such as fish and crustaceans has shown improvements in growth, health intestine and the quality of the final products. Despite these advantages, it is noted that complete replacement of fishmeal with insect meal is not the ultimate solution, as challenges related to regulation, mass insect rearing, and nutrient composition may arise. However, Black Soldier Fly meal stands out as a valuable alternative to diversify and improve feed sustainability in the aquaculture industry, contributing to food safety and care for the environment.

Resumen 16. El crecimiento de la industria acuícola ha sido impulsado por la creciente demanda global de alimentos y la preocupación por la sobreexplotación de recursos naturales y el impacto ambiental. Para abordar estos desafíos, la acuicultura ha buscado alternativas sostenibles e innovadoras. En este contexto, la inclusión de insectos, como la Mosca Soldado Negra (Hermetia illucens), en la dieta de peces y crustáceos surge como una solución prometedora debido a su alto contenido de proteínas, lípidos y minerales. Este artículo de revisión analiza el papel de la harina de larvas de Mosca Soldado Negra en la acuicultura. Examina los beneficios económicos, nutricionales y ambientales de la harina en la dieta de los animales acuáticos, así como los resultados de investigaciones recientes sobre dosificación adecuada, calidad de crecimiento, salud intestinal y disponibilidad de nutrientes en diversas especies acuícolas. Se enfatiza que la harina de larvas de la Mosca Soldado Negra ofrece beneficios nutricionales comparables a la harina de pescado, siendo una fuente rica en proteínas y lípidos, y su inclusión en la dieta de especies acuáticas como peces y crustáceos ha demostrado mejoras en el crecimiento, la salud intestinal y la calidad de los productos finales. A pesar de estas ventajas, se señala que la sustitución completa de la harina de pescado por la harina de insectos no es la solución definitiva, ya que pueden surgir desafíos relacionados con la regulación, la crianza masiva de insectos y la composición de nutrientes. Sin embargo, la harina de larvas de Mosca Soldado Negra se destaca como una alternativa valiosa para diversificar y mejorar la sostenibilidad de la alimentación en la industria acuícola, contribuyendo a la seguridad alimentaria y al cuidado del medio ambiente.

Resumo O presente estudo foi conduzido para investigar a variedade de hospedeiros, distribuição e avaliar a toxicidade de quatro inseticidas: imidaclopride, fipronil, cipermetrina e clorpirifós isoladamente e em combinação contra larva de 3º ínstar e estágio adulto da mosca-das-frutas Bactrocera diversa Coquillett, 1904 (Diptera: Tephritidae) durante 2021. Larvas de B. diversa foram encontradas se alimentando vigorosamente dentro dos hospedeiros de cucurbitáceas (abóbora, pepino, cabaça amarga, melancia, melão redondo, cabaça) coletados em diferentes localidades da divisão Poonch de Azad Jammu e Caxemira, Paquistão, e essa espécie é relatada pela primeira vez como novo registro para essa região. A suscetibilidade de B. diversa a inseticidas foi avaliada por método tópico. A mortalidade foi verificada após 3, 6, 8 e 24 horas de exposição. A cipermetrina foi mais eficaz para matar 50% do estágio larval e adulto com menos CL50 [7,2(1,040 ± 0,214), 17,4 (0,748±0,193)], respectivamente, seguido por imidaclopride. O imidaclopride matou mais efetivamente 90% da população larval e adulta com o menor valor de LC90 [73,2 (3,013 ± 0,708) 16,9 (1,886 ± 0,437)], respectivamente, após 24 horas. Cipermetrina com clorpirifós matou mais efetivamente 50% e 90% do estágio larval e adulto de B. diversa com valor mínimo de LC50 [11,3 (1,085 ± 0,245), 2,5 (0,759 ± 0,252)] e valor mínimo de LC90 [171,3 (1,085 ± 0,245), 121,9 (0,759 ± 0,252)], respectivamente, após 24 horas de exposição. A toxicidade de cada inseticida aumentou com a exposição por mais tempo e com o aumento da dose. A cipermetrina é sugerida como mais eficaz contra os estágios larval e adulto de B. diversa em combinação com clorpirifós seguido de imidaclopride.

Abstract The present study was conducted to investigate the array of hosts, distribution and to evaluate the toxicity of four insecticides: imidacloprid, fipronil, cypermethrin and chlorpyrifos alone and in combination against 3rd instar maggot and adult stage of fruit fly Bactrocera diversa Coquillett, 1904 (Diptera: Tephritidae) during 2021. B. diversa maggots were found vigorously feeding inside the cucurbit hosts (pumpkin, cucumber, bitter gourd, watermelon, round melon, bottle gourd) collected from different localities of Poonch division of Azad Jammu & Kashmir, Pakistan, and this species is reported for the first time as new record to this region. Susceptibilities of B. diversa to insecticides were evaluated using topical method. Mortality was checked after 3, 6, 8 and 24h of exposure. Cypermethrin was most effective to kill 50% of both larval and adult stage with least LC50 [7.2(1.040±0.214), 17.4(0.748±0.193)], respectively followed by imidacloprid. Imidacloprid most effectively killed 90% of both larval and adult population with least LC90 value [73.2 (3.013±0.708) 16.9 (1.886±0.437)] respectively after 24 hours. Cypermethrin with chlorpyrifos most effectively killed 50 and 90 percent of both larval and adult stage of B. diversa with least LC50 value [11.3 (1.085±0.245), 2.5 (0.759±0.252)] and least LC90 value [171.3 (1.085±0.245), 121.9 (0.759±0.252)], respectively after 24h of exposure. Toxicity of each insecticide increased with exposure for longer time and increased dose. Cypermethrin is suggested as most effective against both larval and adult stages of B. diversa in combination with chlorpyrifos followed by imidacloprid.

Resumo A cultura do milho é utilizada na alimentação humana, pecuária e avícola, bem como na panificação, flocos de milho, xarope de milho, amido de milho e óleos de milho. O estudo de campo consistiu em um ensaio experimental sobre a incidência desse complexo de insetos-praga na cultivar de milho Azam durante a temporada de Kharif 2020 na Estação de Pesquisa Agrícola, Baffa, Mansehra. O ensaio foi estabelecido no Randomized Complete Block Design (RCBD) e, em seguida, dividido em três repetições. O resultado obtido no ensaio mostrou que várias espécies de pragas foram registradas durante o período experimental; no entanto, foi observado um baixo nível da população. As espécies de insetos obtidas foram pulgão-da-folha-do-milho (6,90 ± 5,5) – por polegada quadrada –, cigarrinha-do-milho (1,32 ± 0,63), broca-do-caule-do-milho (0,63 ± 0,29), besouro-da-pulga-do-milho (0,43 ± 0,28), tripses (0,38 ± 0,22), lagarta-cabeluda (0,21 ± 0,22), gafanhoto (0,17 ± 0,11) e mosca (0,11 ± 0,08) ao longo da temporada

Abstract The maize crop is used as food for humans, livestock and poultries forms, it is also used in bread making, corn flakes, corn syrup, corn starch and corn oils. The field study consisted of one experimental trial, about the incidence of that insect pest complex on maize cultivar Azam during the Kharif season 2020 at the Agricultural, Research Station, Baffa, Mansehra. The trial was laid out in the Randomized Complete Block Design (RCBD), and then it was divided into three replications. The result obtained from the trial showed that a number of the pest species were recorded during the experimental period; however, the population was noticed at a low level. The obtained insect species were corn leaf aphid (6.90 ± 5.5) per square inch, corn leafhopper (1.32 ± 0.63), maize stem borer (0.63 ± 0.29), corn flea beetle (0.43 ± 0.28), Thrips (0.38 ± 0.22), Hairy caterpillar (0.21 ± 0.22), Grasshopper (0.17 ± 0.11) and shoot fly (0.11 ± 0.08) throughout the season

Resumen Las especies mexicanas de Belonuchus Nordmann se colectan con cierta frecuencia en sustratos con materia orgánica en descomposición o usando trampas con este tipo de cebos y se les considera depredadoras de otros insectos, como larvas de moscas. Se tienen contribuciones recientes sobre su taxonomía; sin embargo, aún hacen falta más estudios al respecto. En este trabajo se describen 2 especies nuevas de Belonuchus procedentes de varios estados de México, las cuales fueron localizadas en el material entomológico de la Colección Nacional de Insectos de la UNAM. Las descripciones se acompañan con imágenes que ayudan al reconocimiento de las especies y se presenta una clave modificada que facilita su identificación. Además, se analizaron ejemplares de algunas especies ya conocidas que representan varios primeros registros estatales. Todo lo anterior ha derivado en el incremento de la riqueza nacional de especies de Belonuchus y en un conocimiento más completo sobre su taxonomía y distribución geográfica.

Abstract The Mexican species of Belonuchus Nordmann are collected with some frequency in substrates with decomposing organic matter or using traps with this type of bait and are considered predators of other insects, such as fly larvae. There are recent contributions on their taxonomy; however, more studies are still needed in this regard. This paper describes 2 new species of Belonuchus from several states in Mexico, which were located in the entomological material of the National Insect Collection, UNAM. Descriptions are accompanied by images that help to recognize the species and a modified key is presented to facilitate their identification. In addition, several specimens of some already known species, which represent first state records, were analyzed. All of the above has resulted in an increase in the national richness of Belonuchus species and a more complete knowledge of its taxonomy and geographic distribution.

The use of protective netting is becoming an increasingly popular practice in the citrus industry in South Africa. However, data on its effects on biotic factors, particularly insect pests, are limited. This study focused on the effect nets have on key citrus pests in the Eastern Cape province. Orchards under nets and open orchards, of similar cultivars, ages and management practices, were monitored at several sites over two seasons for pest infestation and damage. Weekly monitoring was conducted for Thaumatotibia leucotreta infestation. Other pests were monitored either monthly or once a season. During the first season, T. leucotreta infestation was higher in orchards under nets, probably because, unlike the open orchards, the nets provided protection for the existing high levels of T. leucotreta. No T. leucotreta infestation was recorded in both orchard types in 2019. This was due to generally lower than usual pest abundance and dramatically improved area-wide management of T. leucotreta. Pheromone traps were used to monitor T. leucotreta males, including sterile moths used in a sterile insect technique programme. Although higher catches of wild T. leucotreta moths were recorded in orchards under nets, so too were there higher numbers of sterile moths and a higher ratio of sterile to wild moths, indicating the potential for better pest suppression for orchards under nets. Various other key pests were monitored during this time, with variable results for each species. Pests that were elevated under nets include Planococcus citri and Aonidiella aurantii. Pests that seemed to be lower under nets were Ceratitis capitata and Scirtothrips aurantii. Nets had no effect on Empoasca distinguenda, Penthimiola bella and Eriophyes sheldoni. These differences in pest levels in netted and open orchards trigger an important debate on whether nets are beneficial for or detrimental to the successful implementation of an integrated pest management programme.

Trypanosomosis is a disease complex which affects both humans and animals in sub-Saharan Africa, transmitted by the tsetse fly and distributed within the tsetse belt of Africa. But some trypanosome species, for example, Trypanosoma brucei evansi, T. vivax, T. theileri and T. b. equiperdum are endemic outside the tsetse belt of Africa transmitted by biting flies, for example, Tabanus and Stomoxys, or venereal transmission, respectively. Trypanocidal drugs remain the principal method of animal trypanosomosis control in most African countries. However, there is a growing concern that their effectiveness may be severely curtailed by widespread drug resistance. A minimum number of six male cattle calves were recruited for the study. They were randomly grouped into two (T. vivax and T. congolense groups) of three calves each. One calf per group served as a control while two calves were treatment group. They were inoculated with 10(5) cells/mL parasites in phosphate buffered solution (PBS) in 2 mL. When parasitaemia reached 1 × 10(7.8) cells/mL trypanosomes per mL in calves, treatment was instituted with 20 mL (25 mg/kg in 100 kg calf) ascofuranone (AF) for treatment calves, while the control ones were administered a placebo (20 mL PBS) intramuscularly. This study revealed that T. vivax was successfully cleared by AF but the T. congolense group was not cleared effectively. CONTRIBUTION: There is an urgent need to develop new drugs which this study sought to address. It is suggested that the AF compound can be developed further to be a sanative drug for T. vivax in non-tsetse infested areas like South Americas